SEQUENCE LISTING < 110 > MODERNATX , INC < 120 > IMMUNOMODULATORY THERAPEUTIC MRNA COMPOSITIONS ENCODING ACTIVATING ONCOGENE MUTATION PEPTIDES < 130 > MDN - 015 PC < 140 > PCT / US 2018 / 016510 < 141 > 2018 - 02 - 01 < 150 > US 62 / 453 , 465 < 151 > 2017 - 02 - 01 < 150 > US 62 / 467 , 063 < 151 > 2017 - 03 - 03 < 150 > US 62 / 490 , 523 < 151 > 2017 - 04 - 26 < 150 > US 62 / 541 , 571 < 151 > 2017 - 08 - 04 < 160 > 232 < 170 > PatentIn version 3 . 5 < 210 > 1 < 211 > 379 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : huSTING ( V 155 M ) ; no epitope tag < 400 > 1 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 370 375 < 210 > 2 < 211 > 379 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 284 T ) ; no epitope tag < 400 > 2 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Val Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Thr Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 370 375 < 210 > 3 < 211 > 379 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : hu STING ( R 284 M ) ; no epitope tag < 400 > 3 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Val Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Met Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 370 375 < 210 > 4 < 211 > 379 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 284 K ) ; no epitope tag < 400 > 4 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Val Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Lys Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 370 375 < 210 > 5 < 211 > 379 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( N 154 S ) ; no epitope tag < 400 > 5 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Ser Val Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 370 375 < 210 > 6 < 211 > 379 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( V 147 L ) ; no epitope tag < 400 > 6 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Leu Cys Glu Lys Gly Asn Phe Asn Val Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 370 375 < 210 > 7 < 211 > 379 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( E 315 Q ) ; no epitope tag < 400 > 7 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Val Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Gln Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 370 375 < 210 > 8 < 211 > 379 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 375 A ) ; no epitope tag < 400 > 8 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Val Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Ala Thr Asp Phe Ser 370 375 < 210 > 9 < 211 > 379 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( V 147 L / N 154 S / V 155 M ) ; no epitope tag < 400 > 9 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Leu Cys Glu Lys Gly Asn Phe Ser Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 370 375 < 210 > 10 < 211 > 379 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 284 M / V 147 L / N 154 S / V 155 M ) ; no epitope tag < 400 > 10 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Leu Cys Glu Lys Gly Asn Phe Ser Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Met Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 370 375 < 210 > 11 < 211 > 419 < 212 > PRT < 213 > Mus musculus < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 419 ) < 223 > super mouse IRF 3 S 396 D ; no epitope tag < 400 > 11 Met Glu Thr Pro Lys Pro Arg Ile Leu Pro Trp Leu Val Ser Gln Leu 1 5 10 15 Asp Leu Gly Gln Leu Glu Gly Val Ala Trp Leu Asp Glu Ser Arg Thr 20 25 30 Arg Phe Arg Ile Pro Trp Lys His Gly Leu Arg Gln Asp Ala Gln Met 35 40 45 Ala Asp Phe Gly Ile Phe Gln Ala Trp Ala Glu Ala Ser Gly Ala Tyr 50 55 60 Thr Pro Gly Lys Asp Lys Pro Asp Val Ser Thr Trp Lys Arg Asn Phe 65 70 75 80 Arg Ser Ala Leu Asn Arg Lys Glu Val Leu Arg Leu Ala Ala Asp Asn 85 90 95 Ser Lys Asp Pro Tyr Asp Pro His Lys Val Tyr Glu Phe Val Thr Pro 100 105 110 Gly Ala Arg Asp Phe Val His Leu Gly Ala Ser Pro Asp Thr Asn Gly 115 120 125 Lys Ser Ser Leu Pro His Ser Gln Glu Asn Leu Pro Lys Leu Phe Asp 130 135 140 Gly Leu Ile Leu Gly Pro Leu Lys Asp Glu Gly Ser Ser Asp Leu Ala 145 150 155 160 Ile Val Ser Asp Pro Ser Gln Gln Leu Pro Ser Pro Asn Val Asn Asn 165 170 175 Phe Leu Asn Pro Ala Pro Gln Glu Asn Pro Leu Lys Gln Leu Leu Ala 180 185 190 Glu Glu Gln Trp Glu Phe Glu Val Thr Ala Phe Tyr Arg Gly Arg Gln 195 200 205 Val Phe Gln Gln Thr Leu Phe Cys Pro Gly Gly Leu Arg Leu Val Gly 210 215 220 Ser Thr Ala Asp Met Thr Leu Pro Trp Gln Pro Val Thr Leu Pro Asp 225 230 235 240 Pro Glu Gly Phe Leu Thr Asp Lys Leu Val Lys Glu Tyr Val Gly Gln 245 250 255 Val Leu Lys Gly Leu Gly Asn Gly Leu Ala Leu Trp Gln Ala Gly Gln 260 265 270 Cys Leu Trp Ala Gln Arg Leu Gly His Ser His Ala Phe Trp Ala Leu 275 280 285 Gly Glu Glu Leu Leu Pro Asp Ser Gly Arg Gly Pro Asp Gly Glu Val 290 295 300 His Lys Asp Lys Asp Gly Ala Val Phe Asp Leu Arg Pro Phe Val Ala 305 310 315 320 Asp Leu Ile Ala Phe Met Glu Gly Ser Gly His Ser Pro Arg Tyr Thr 325 330 335 Leu Trp Phe Cys Met Gly Glu Met Trp Pro Gln Asp Gln Pro Trp Val 340 345 350 Lys Arg Leu Val Met Val Lys Val Val Pro Thr Cys Leu Lys Glu Leu 355 360 365 Leu Glu Met Ala Arg Glu Gly Gly Ala Ser Ser Leu Lys Thr Val Asp 370 375 380 Leu His Ile Asp Asn Ser Gln Pro Ile Ser Leu Thr Ser Asp Gln Tyr 385 390 395 400 Lys Ala Tyr Leu Gln Asp Leu Val Glu Asp Met Asp Phe Gln Ala Thr 405 410 415 Gly Asn Ile < 210 > 12 < 211 > 427 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 427 ) < 223 > super human IRF 3 S 396 D ; no epitope tag < 400 > 12 Met Gly Thr Pro Lys Pro Arg Ile Leu Pro Trp Leu Val Ser Gln Leu 1 5 10 15 Asp Leu Gly Gln Leu Glu Gly Val Ala Trp Val Asn Lys Ser Arg Thr 20 25 30 Arg Phe Arg Ile Pro Trp Lys His Gly Leu Arg Gln Asp Ala Gln Gln 35 40 45 Glu Asp Phe Gly Ile Phe Gln Ala Trp Ala Glu Ala Thr Gly Ala Tyr 50 55 60 Val Pro Gly Arg Asp Lys Pro Asp Leu Pro Thr Trp Lys Arg Asn Phe 65 70 75 80 Arg Ser Ala Leu Asn Arg Lys Glu Gly Leu Arg Leu Ala Glu Asp Arg 85 90 95 Ser Lys Asp Pro His Asp Pro His Lys Ile Tyr Glu Phe Val Asn Ser 100 105 110 Gly Val Gly Asp Phe Ser Gln Pro Asp Thr Ser Pro Asp Thr Asn Gly 115 120 125 Gly Gly Ser Thr Ser Asp Thr Gln Glu Asp Ile Leu Asp Glu Leu Leu 130 135 140 Gly Asn Met Val Leu Ala Pro Leu Pro Asp Pro Gly Pro Pro Ser Leu 145 150 155 160 Ala Val Ala Pro Glu Pro Cys Pro Gln Pro Leu Arg Ser Pro Ser Leu 165 170 175 Asp Asn Pro Thr Pro Phe Pro Asn Leu Gly Pro Ser Glu Asn Pro Leu 180 185 190 Lys Arg Leu Leu Val Pro Gly Glu Glu Trp Glu Phe Glu Val Thr Ala 195 200 205 Phe Tyr Arg Gly Arg Gln Val Phe Gln Gln Thr Ile Ser Cys Pro Glu 210 215 220 Gly Leu Arg Leu Val Gly Ser Glu Val Gly Asp Arg Thr Leu Pro Gly 225 230 235 240 Trp Pro Val Thr Leu Pro Asp Pro Gly Met Ser Leu Thr Asp Arg Gly 245 250 255 Val Met Ser Tyr Val Arg His Val Leu Ser Cys Leu Gly Gly Gly Leu 260 265 270 Ala Leu Trp Arg Ala Gly Gln Trp Leu Trp Ala Gln Arg Leu Gly His 275 280 285 Cys His Thr Tyr Trp Ala Val Ser Glu Glu Leu Leu Pro Asn Ser Gly 290 295 300 His Gly Pro Asp Gly Glu Val Pro Lys Asp Lys Glu Gly Gly Val Phe 305 310 315 320 Asp Leu Gly Pro Phe Ile Val Asp Leu Ile Thr Phe Thr Glu Gly Ser 325 330 335 Gly Arg Ser Pro Arg Tyr Ala Leu Trp Phe Cys Val Gly Glu Ser Trp 340 345 350 Pro Gln Asp Gln Pro Trp Thr Lys Arg Leu Val Met Val Lys Val Val 355 360 365 Pro Thr Cys Leu Arg Ala Leu Val Glu Met Ala Arg Val Gly Gly Ala 370 375 380 Ser Ser Leu Glu Asn Thr Val Asp Leu His Ile Asp Asn Ser His Pro 385 390 395 400 Leu Ser Leu Thr Ser Asp Gln Tyr Lys Ala Tyr Leu Gln Asp Leu Val 405 410 415 Glu Gly Met Asp Phe Gln Gly Pro Gly Glu Thr 420 425 < 210 > 13 < 211 > 503 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Wild - type Hu IRF 7 isoform A ; P 037 without epitope tag < 400 > 13 Met Ala Leu Ala Pro Glu Arg Ala Ala Pro Arg Val Leu Phe Gly Glu 1 5 10 15 Trp Leu Leu Gly Glu Ile Ser Ser Gly Cys Tyr Glu Gly Leu Gln Trp 20 25 30 Leu Asp Glu Ala Arg Thr Cys Phe Arg Val Pro Trp Lys His Phe Ala 35 40 45 Arg Lys Asp Leu Ser Glu Ala Asp Ala Arg Ile Phe Lys Ala Trp Ala 50 55 60 Val Ala Arg Gly Arg Trp Pro Pro Ser Ser Arg Gly Gly Gly Pro Pro 65 70 75 80 Pro Glu Ala Glu Thr Ala Glu Arg Ala Gly Trp Lys Thr Asn Phe Arg 85 90 95 Cys Ala Leu Arg Ser Thr Arg Arg Phe Val Met Leu Arg Asp Asn Ser 100 105 110 Gly Asp Pro Ala Asp Pro His Lys Val Tyr Ala Leu Ser Arg Glu Leu 115 120 125 Cys Trp Arg Glu Gly Pro Gly Thr Asp Gln Thr Glu Ala Glu Ala Pro 130 135 140 Ala Ala Val Pro Pro Pro Gln Gly Gly Pro Pro Gly Pro Phe Leu Ala 145 150 155 160 His Thr His Ala Gly Leu Gln Ala Pro Gly Pro Leu Pro Ala Pro Ala 165 170 175 Gly Asp Lys Gly Asp Leu Leu Leu Gln Ala Val Gln Gln Ser Cys Leu 180 185 190 Ala Asp His Leu Leu Thr Ala Ser Trp Gly Ala Asp Pro Val Pro Thr 195 200 205 Lys Ala Pro Gly Glu Gly Gln Glu Gly Leu Pro Leu Thr Gly Ala Cys 210 215 220 Ala Gly Gly Pro Gly Leu Pro Ala Gly Glu Leu Tyr Gly Trp Ala Val 225 230 235 240 Glu Thr Thr Pro Ser Pro Gly Pro Gln Pro Ala Ala Leu Thr Thr Gly 245 250 255 Glu Ala Ala Ala Pro Glu Ser Pro His Gln Ala Glu Pro Tyr Leu Ser 260 265 270 Pro Ser Pro Ser Ala Cys Thr Ala Val Gln Glu Pro Ser Pro Gly Ala 275 280 285 Leu Asp Val Thr Ile Met Tyr Lys Gly Arg Thr Val Leu Gln Lys Val 290 295 300 Val Gly His Pro Ser Cys Thr Phe Leu Tyr Gly Pro Pro Asp Pro Ala 305 310 315 320 Val Arg Ala Thr Asp Pro Gln Gln Val Ala Phe Pro Ser Pro Ala Glu 325 330 335 Leu Pro Asp Gln Lys Gln Leu Arg Tyr Thr Glu Glu Leu Leu Arg His 340 345 350 Val Ala Pro Gly Leu His Leu Glu Leu Arg Gly Pro Gln Leu Trp Ala 355 360 365 Arg Arg Met Gly Lys Cys Lys Val Tyr Trp Glu Val Gly Gly Pro Pro 370 375 380 Gly Ser Ala Ser Pro Ser Thr Pro Ala Cys Leu Leu Pro Arg Asn Cys 385 390 395 400 Asp Thr Pro Ile Phe Asp Phe Arg Val Phe Phe Gln Glu Leu Val Glu 405 410 415 Phe Arg Ala Arg Gln Arg Arg Gly Ser Pro Arg Tyr Thr Ile Tyr Leu 420 425 430 Gly Phe Gly Gln Asp Leu Ser Ala Gly Arg Pro Lys Glu Lys Ser Leu 435 440 445 Val Leu Val Lys Leu Glu Pro Trp Leu Cys Arg Val His Leu Glu Gly 450 455 460 Thr Gln Arg Glu Gly Val Ser Ser Leu Asp Ser Ser Ser Leu Ser Leu 465 470 475 480 Cys Leu Ser Ser Ala Asn Ser Leu Tyr Asp Asp Ile Glu Cys Phe Leu 485 490 495 Met Glu Leu Glu Gln Pro Ala 500 < 210 > 14 < 211 > 503 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active Hu IRF 7 S 477 D / S 479 D ; P 033 without epitope tag < 400 > 14 Met Ala Leu Ala Pro Glu Arg Ala Ala Pro Arg Val Leu Phe Gly Glu 1 5 10 15 Trp Leu Leu Gly Glu Ile Ser Ser Gly Cys Tyr Glu Gly Leu Gln Trp 20 25 30 Leu Asp Glu Ala Arg Thr Cys Phe Arg Val Pro Trp Lys His Phe Ala 35 40 45 Arg Lys Asp Leu Ser Glu Ala Asp Ala Arg Ile Phe Lys Ala Trp Ala 50 55 60 Val Ala Arg Gly Arg Trp Pro Pro Ser Ser Arg Gly Gly Gly Pro Pro 65 70 75 80 Pro Glu Ala Glu Thr Ala Glu Arg Ala Gly Trp Lys Thr Asn Phe Arg 85 90 95 Cys Ala Leu Arg Ser Thr Arg Arg Phe Val Met Leu Arg Asp Asn Ser 100 105 110 Gly Asp Pro Ala Asp Pro His Lys Val Tyr Ala Leu Ser Arg Glu Leu 115 120 125 Cys Trp Arg Glu Gly Pro Gly Thr Asp Gln Thr Glu Ala Glu Ala Pro 130 135 140 Ala Ala Val Pro Pro Pro Gln Gly Gly Pro Pro Gly Pro Phe Leu Ala 145 150 155 160 His Thr His Ala Gly Leu Gln Ala Pro Gly Pro Leu Pro Ala Pro Ala 165 170 175 Gly Asp Lys Gly Asp Leu Leu Leu Gln Ala Val Gln Gln Ser Cys Leu 180 185 190 Ala Asp His Leu Leu Thr Ala Ser Trp Gly Ala Asp Pro Val Pro Thr 195 200 205 Lys Ala Pro Gly Glu Gly Gln Glu Gly Leu Pro Leu Thr Gly Ala Cys 210 215 220 Ala Gly Gly Pro Gly Leu Pro Ala Gly Glu Leu Tyr Gly Trp Ala Val 225 230 235 240 Glu Thr Thr Pro Ser Pro Gly Pro Gln Pro Ala Ala Leu Thr Thr Gly 245 250 255 Glu Ala Ala Ala Pro Glu Ser Pro His Gln Ala Glu Pro Tyr Leu Ser 260 265 270 Pro Ser Pro Ser Ala Cys Thr Ala Val Gln Glu Pro Ser Pro Gly Ala 275 280 285 Leu Asp Val Thr Ile Met Tyr Lys Gly Arg Thr Val Leu Gln Lys Val 290 295 300 Val Gly His Pro Ser Cys Thr Phe Leu Tyr Gly Pro Pro Asp Pro Ala 305 310 315 320 Val Arg Ala Thr Asp Pro Gln Gln Val Ala Phe Pro Ser Pro Ala Glu 325 330 335 Leu Pro Asp Gln Lys Gln Leu Arg Tyr Thr Glu Glu Leu Leu Arg His 340 345 350 Val Ala Pro Gly Leu His Leu Glu Leu Arg Gly Pro Gln Leu Trp Ala 355 360 365 Arg Arg Met Gly Lys Cys Lys Val Tyr Trp Glu Val Gly Gly Pro Pro 370 375 380 Gly Ser Ala Ser Pro Ser Thr Pro Ala Cys Leu Leu Pro Arg Asn Cys 385 390 395 400 Asp Thr Pro Ile Phe Asp Phe Arg Val Phe Phe Gln Glu Leu Val Glu 405 410 415 Phe Arg Ala Arg Gln Arg Arg Gly Ser Pro Arg Tyr Thr Ile Tyr Leu 420 425 430 Gly Phe Gly Gln Asp Leu Ser Ala Gly Arg Pro Lys Glu Lys Ser Leu 435 440 445 Val Leu Val Lys Leu Glu Pro Trp Leu Cys Arg Val His Leu Glu Gly 450 455 460 Thr Gln Arg Glu Gly Val Ser Ser Leu Asp Ser Ser Asp Leu Asp Leu 465 470 475 480 Cys Leu Ser Ser Ala Asn Ser Leu Tyr Asp Asp Ile Glu Cys Phe Leu 485 490 495 Met Glu Leu Glu Gln Pro Ala 500 < 210 > 15 < 211 > 503 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active Hu IRF 7 S 475 D / S 477 D / L 480 D ; P 034 without epitope tag < 400 > 15 Met Ala Leu Ala Pro Glu Arg Ala Ala Pro Arg Val Leu Phe Gly Glu 1 5 10 15 Trp Leu Leu Gly Glu Ile Ser Ser Gly Cys Tyr Glu Gly Leu Gln Trp 20 25 30 Leu Asp Glu Ala Arg Thr Cys Phe Arg Val Pro Trp Lys His Phe Ala 35 40 45 Arg Lys Asp Leu Ser Glu Ala Asp Ala Arg Ile Phe Lys Ala Trp Ala 50 55 60 Val Ala Arg Gly Arg Trp Pro Pro Ser Ser Arg Gly Gly Gly Pro Pro 65 70 75 80 Pro Glu Ala Glu Thr Ala Glu Arg Ala Gly Trp Lys Thr Asn Phe Arg 85 90 95 Cys Ala Leu Arg Ser Thr Arg Arg Phe Val Met Leu Arg Asp Asn Ser 100 105 110 Gly Asp Pro Ala Asp Pro His Lys Val Tyr Ala Leu Ser Arg Glu Leu 115 120 125 Cys Trp Arg Glu Gly Pro Gly Thr Asp Gln Thr Glu Ala Glu Ala Pro 130 135 140 Ala Ala Val Pro Pro Pro Gln Gly Gly Pro Pro Gly Pro Phe Leu Ala 145 150 155 160 His Thr His Ala Gly Leu Gln Ala Pro Gly Pro Leu Pro Ala Pro Ala 165 170 175 Gly Asp Lys Gly Asp Leu Leu Leu Gln Ala Val Gln Gln Ser Cys Leu 180 185 190 Ala Asp His Leu Leu Thr Ala Ser Trp Gly Ala Asp Pro Val Pro Thr 195 200 205 Lys Ala Pro Gly Glu Gly Gln Glu Gly Leu Pro Leu Thr Gly Ala Cys 210 215 220 Ala Gly Gly Pro Gly Leu Pro Ala Gly Glu Leu Tyr Gly Trp Ala Val 225 230 235 240 Glu Thr Thr Pro Ser Pro Gly Pro Gln Pro Ala Ala Leu Thr Thr Gly 245 250 255 Glu Ala Ala Ala Pro Glu Ser Pro His Gln Ala Glu Pro Tyr Leu Ser 260 265 270 Pro Ser Pro Ser Ala Cys Thr Ala Val Gln Glu Pro Ser Pro Gly Ala 275 280 285 Leu Asp Val Thr Ile Met Tyr Lys Gly Arg Thr Val Leu Gln Lys Val 290 295 300 Val Gly His Pro Ser Cys Thr Phe Leu Tyr Gly Pro Pro Asp Pro Ala 305 310 315 320 Val Arg Ala Thr Asp Pro Gln Gln Val Ala Phe Pro Ser Pro Ala Glu 325 330 335 Leu Pro Asp Gln Lys Gln Leu Arg Tyr Thr Glu Glu Leu Leu Arg His 340 345 350 Val Ala Pro Gly Leu His Leu Glu Leu Arg Gly Pro Gln Leu Trp Ala 355 360 365 Arg Arg Met Gly Lys Cys Lys Val Tyr Trp Glu Val Gly Gly Pro Pro 370 375 380 Gly Ser Ala Ser Pro Ser Thr Pro Ala Cys Leu Leu Pro Arg Asn Cys 385 390 395 400 Asp Thr Pro Ile Phe Asp Phe Arg Val Phe Phe Gln Glu Leu Val Glu 405 410 415 Phe Arg Ala Arg Gln Arg Arg Gly Ser Pro Arg Tyr Thr Ile Tyr Leu 420 425 430 Gly Phe Gly Gln Asp Leu Ser Ala Gly Arg Pro Lys Glu Lys Ser Leu 435 440 445 Val Leu Val Lys Leu Glu Pro Trp Leu Cys Arg Val His Leu Glu Gly 450 455 460 Thr Gln Arg Glu Gly Val Ser Ser Leu Asp Asp Ser Asp Leu Ser Asp 465 470 475 480 Cys Leu Ser Ser Ala Asn Ser Leu Tyr Asp Asp Ile Glu Cys Phe Leu 485 490 495 Met Glu Leu Glu Gln Pro Ala 500 < 210 > 16 < 211 > 503 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active Hu IRF 7 S 475 D / S 476 D / S 477 D / S 479 D / S 483 D / S 487 D ; P 035 without epitope tag < 400 > 16 Met Ala Leu Ala Pro Glu Arg Ala Ala Pro Arg Val Leu Phe Gly Glu 1 5 10 15 Trp Leu Leu Gly Glu Ile Ser Ser Gly Cys Tyr Glu Gly Leu Gln Trp 20 25 30 Leu Asp Glu Ala Arg Thr Cys Phe Arg Val Pro Trp Lys His Phe Ala 35 40 45 Arg Lys Asp Leu Ser Glu Ala Asp Ala Arg Ile Phe Lys Ala Trp Ala 50 55 60 Val Ala Arg Gly Arg Trp Pro Pro Ser Ser Arg Gly Gly Gly Pro Pro 65 70 75 80 Pro Glu Ala Glu Thr Ala Glu Arg Ala Gly Trp Lys Thr Asn Phe Arg 85 90 95 Cys Ala Leu Arg Ser Thr Arg Arg Phe Val Met Leu Arg Asp Asn Ser 100 105 110 Gly Asp Pro Ala Asp Pro His Lys Val Tyr Ala Leu Ser Arg Glu Leu 115 120 125 Cys Trp Arg Glu Gly Pro Gly Thr Asp Gln Thr Glu Ala Glu Ala Pro 130 135 140 Ala Ala Val Pro Pro Pro Gln Gly Gly Pro Pro Gly Pro Phe Leu Ala 145 150 155 160 His Thr His Ala Gly Leu Gln Ala Pro Gly Pro Leu Pro Ala Pro Ala 165 170 175 Gly Asp Lys Gly Asp Leu Leu Leu Gln Ala Val Gln Gln Ser Cys Leu 180 185 190 Ala Asp His Leu Leu Thr Ala Ser Trp Gly Ala Asp Pro Val Pro Thr 195 200 205 Lys Ala Pro Gly Glu Gly Gln Glu Gly Leu Pro Leu Thr Gly Ala Cys 210 215 220 Ala Gly Gly Pro Gly Leu Pro Ala Gly Glu Leu Tyr Gly Trp Ala Val 225 230 235 240 Glu Thr Thr Pro Ser Pro Gly Pro Gln Pro Ala Ala Leu Thr Thr Gly 245 250 255 Glu Ala Ala Ala Pro Glu Ser Pro His Gln Ala Glu Pro Tyr Leu Ser 260 265 270 Pro Ser Pro Ser Ala Cys Thr Ala Val Gln Glu Pro Ser Pro Gly Ala 275 280 285 Leu Asp Val Thr Ile Met Tyr Lys Gly Arg Thr Val Leu Gln Lys Val 290 295 300 Val Gly His Pro Ser Cys Thr Phe Leu Tyr Gly Pro Pro Asp Pro Ala 305 310 315 320 Val Arg Ala Thr Asp Pro Gln Gln Val Ala Phe Pro Ser Pro Ala Glu 325 330 335 Leu Pro Asp Gln Lys Gln Leu Arg Tyr Thr Glu Glu Leu Leu Arg His 340 345 350 Val Ala Pro Gly Leu His Leu Glu Leu Arg Gly Pro Gln Leu Trp Ala 355 360 365 Arg Arg Met Gly Lys Cys Lys Val Tyr Trp Glu Val Gly Gly Pro Pro 370 375 380 Gly Ser Ala Ser Pro Ser Thr Pro Ala Cys Leu Leu Pro Arg Asn Cys 385 390 395 400 Asp Thr Pro Ile Phe Asp Phe Arg Val Phe Phe Gln Glu Leu Val Glu 405 410 415 Phe Arg Ala Arg Gln Arg Arg Gly Ser Pro Arg Tyr Thr Ile Tyr Leu 420 425 430 Gly Phe Gly Gln Asp Leu Ser Ala Gly Arg Pro Lys Glu Lys Ser Leu 435 440 445 Val Leu Val Lys Leu Glu Pro Trp Leu Cys Arg Val His Leu Glu Gly 450 455 460 Thr Gln Arg Glu Gly Val Ser Ser Leu Asp Asp Asp Asp Leu Asp Leu 465 470 475 480 Cys Leu Asp Ser Ala Asn Asp Leu Tyr Asp Asp Ile Glu Cys Phe Leu 485 490 495 Met Glu Leu Glu Gln Pro Ala 500 < 210 > 17 < 211 > 282 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active truncated Hu IRF 7 1 - 246 + 468 - 503 ; P 032 without epitope tag < 400 > 17 Met Ala Leu Ala Pro Glu Arg Ala Ala Pro Arg Val Leu Phe Gly Glu 1 5 10 15 Trp Leu Leu Gly Glu Ile Ser Ser Gly Cys Tyr Glu Gly Leu Gln Trp 20 25 30 Leu Asp Glu Ala Arg Thr Cys Phe Arg Val Pro Trp Lys His Phe Ala 35 40 45 Arg Lys Asp Leu Ser Glu Ala Asp Ala Arg Ile Phe Lys Ala Trp Ala 50 55 60 Val Ala Arg Gly Arg Trp Pro Pro Ser Ser Arg Gly Gly Gly Pro Pro 65 70 75 80 Pro Glu Ala Glu Thr Ala Glu Arg Ala Gly Trp Lys Thr Asn Phe Arg 85 90 95 Cys Ala Leu Arg Ser Thr Arg Arg Phe Val Met Leu Arg Asp Asn Ser 100 105 110 Gly Asp Pro Ala Asp Pro His Lys Val Tyr Ala Leu Ser Arg Glu Leu 115 120 125 Cys Trp Arg Glu Gly Pro Gly Thr Asp Gln Thr Glu Ala Glu Ala Pro 130 135 140 Ala Ala Val Pro Pro Pro Gln Gly Gly Pro Pro Gly Pro Phe Leu Ala 145 150 155 160 His Thr His Ala Gly Leu Gln Ala Pro Gly Pro Leu Pro Ala Pro Ala 165 170 175 Gly Asp Lys Gly Asp Leu Leu Leu Gln Ala Val Gln Gln Ser Cys Leu 180 185 190 Ala Asp His Leu Leu Thr Ala Ser Trp Gly Ala Asp Pro Val Pro Thr 195 200 205 Lys Ala Pro Gly Glu Gly Gln Glu Gly Leu Pro Leu Thr Gly Ala Cys 210 215 220 Ala Gly Gly Pro Gly Leu Pro Ala Gly Glu Leu Tyr Gly Trp Ala Val 225 230 235 240 Glu Thr Thr Pro Ser Pro Glu Gly Val Ser Ser Leu Asp Ser Ser Ser 245 250 255 Leu Ser Leu Cys Leu Ser Ser Ala Asn Ser Leu Tyr Asp Asp Ile Glu 260 265 270 Cys Phe Leu Met Glu Leu Glu Gln Pro Ala 275 280 < 210 > 18 < 211 > 282 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active truncated Hu IRF 7 1 - 246 + 468 - 503 plus S 475 D / S 476 D / S 477 D / S 479 D / S 483 D / S 487 D ; P 036 without epitope tag < 400 > 18 Met Ala Leu Ala Pro Glu Arg Ala Ala Pro Arg Val Leu Phe Gly Glu 1 5 10 15 Trp Leu Leu Gly Glu Ile Ser Ser Gly Cys Tyr Glu Gly Leu Gln Trp 20 25 30 Leu Asp Glu Ala Arg Thr Cys Phe Arg Val Pro Trp Lys His Phe Ala 35 40 45 Arg Lys Asp Leu Ser Glu Ala Asp Ala Arg Ile Phe Lys Ala Trp Ala 50 55 60 Val Ala Arg Gly Arg Trp Pro Pro Ser Ser Arg Gly Gly Gly Pro Pro 65 70 75 80 Pro Glu Ala Glu Thr Ala Glu Arg Ala Gly Trp Lys Thr Asn Phe Arg 85 90 95 Cys Ala Leu Arg Ser Thr Arg Arg Phe Val Met Leu Arg Asp Asn Ser 100 105 110 Gly Asp Pro Ala Asp Pro His Lys Val Tyr Ala Leu Ser Arg Glu Leu 115 120 125 Cys Trp Arg Glu Gly Pro Gly Thr Asp Gln Thr Glu Ala Glu Ala Pro 130 135 140 Ala Ala Val Pro Pro Pro Gln Gly Gly Pro Pro Gly Pro Phe Leu Ala 145 150 155 160 His Thr His Ala Gly Leu Gln Ala Pro Gly Pro Leu Pro Ala Pro Ala 165 170 175 Gly Asp Lys Gly Asp Leu Leu Leu Gln Ala Val Gln Gln Ser Cys Leu 180 185 190 Ala Asp His Leu Leu Thr Ala Ser Trp Gly Ala Asp Pro Val Pro Thr 195 200 205 Lys Ala Pro Gly Glu Gly Gln Glu Gly Leu Pro Leu Thr Gly Ala Cys 210 215 220 Ala Gly Gly Pro Gly Leu Pro Ala Gly Glu Leu Tyr Gly Trp Ala Val 225 230 235 240 Glu Thr Thr Pro Ser Pro Glu Gly Val Ser Ser Leu Asp Asp Asp Asp 245 250 255 Leu Asp Leu Cys Leu Asp Ser Ala Asn Asp Leu Tyr Asp Asp Ile Glu 260 265 270 Cys Phe Leu Met Glu Leu Glu Gln Pro Ala 275 280 < 210 > 19 < 211 > 408 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : truncated Hu IRF 7 1 - 151 + 247 - 503 ; P 038 without epitope tag ; null mutation < 400 > 19 Met Ala Leu Ala Pro Glu Arg Ala Ala Pro Arg Val Leu Phe Gly Glu 1 5 10 15 Trp Leu Leu Gly Glu Ile Ser Ser Gly Cys Tyr Glu Gly Leu Gln Trp 20 25 30 Leu Asp Glu Ala Arg Thr Cys Phe Arg Val Pro Trp Lys His Phe Ala 35 40 45 Arg Lys Asp Leu Ser Glu Ala Asp Ala Arg Ile Phe Lys Ala Trp Ala 50 55 60 Val Ala Arg Gly Arg Trp Pro Pro Ser Ser Arg Gly Gly Gly Pro Pro 65 70 75 80 Pro Glu Ala Glu Thr Ala Glu Arg Ala Gly Trp Lys Thr Asn Phe Arg 85 90 95 Cys Ala Leu Arg Ser Thr Arg Arg Phe Val Met Leu Arg Asp Asn Ser 100 105 110 Gly Asp Pro Ala Asp Pro His Lys Val Tyr Ala Leu Ser Arg Glu Leu 115 120 125 Cys Trp Arg Glu Gly Pro Gly Thr Asp Gln Thr Glu Ala Glu Ala Pro 130 135 140 Ala Ala Val Pro Pro Pro Gln Gly Pro Gln Pro Ala Ala Leu Thr Thr 145 150 155 160 Gly Glu Ala Ala Ala Pro Glu Ser Pro His Gln Ala Glu Pro Tyr Leu 165 170 175 Ser Pro Ser Pro Ser Ala Cys Thr Ala Val Gln Glu Pro Ser Pro Gly 180 185 190 Ala Leu Asp Val Thr Ile Met Tyr Lys Gly Arg Thr Val Leu Gln Lys 195 200 205 Val Val Gly His Pro Ser Cys Thr Phe Leu Tyr Gly Pro Pro Asp Pro 210 215 220 Ala Val Arg Ala Thr Asp Pro Gln Gln Val Ala Phe Pro Ser Pro Ala 225 230 235 240 Glu Leu Pro Asp Gln Lys Gln Leu Arg Tyr Thr Glu Glu Leu Leu Arg 245 250 255 His Val Ala Pro Gly Leu His Leu Glu Leu Arg Gly Pro Gln Leu Trp 260 265 270 Ala Arg Arg Met Gly Lys Cys Lys Val Tyr Trp Glu Val Gly Gly Pro 275 280 285 Pro Gly Ser Ala Ser Pro Ser Thr Pro Ala Cys Leu Leu Pro Arg Asn 290 295 300 Cys Asp Thr Pro Ile Phe Asp Phe Arg Val Phe Phe Gln Glu Leu Val 305 310 315 320 Glu Phe Arg Ala Arg Gln Arg Arg Gly Ser Pro Arg Tyr Thr Ile Tyr 325 330 335 Leu Gly Phe Gly Gln Asp Leu Ser Ala Gly Arg Pro Lys Glu Lys Ser 340 345 350 Leu Val Leu Val Lys Leu Glu Pro Trp Leu Cys Arg Val His Leu Glu 355 360 365 Gly Thr Gln Arg Glu Gly Val Ser Ser Leu Asp Ser Ser Ser Leu Ser 370 375 380 Leu Cys Leu Ser Ser Ala Asn Ser Leu Tyr Asp Asp Ile Glu Cys Phe 385 390 395 400 Leu Met Glu Leu Glu Gln Pro Ala 405 < 210 > 20 < 211 > 353 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : truncated Hu IRF 7 152 - 503 ; P 039 without epitope tag ; null mutation < 400 > 20 Met Gly Gly Pro Pro Gly Pro Phe Leu Ala His Thr His Ala Gly Leu 1 5 10 15 Gln Ala Pro Gly Pro Leu Pro Ala Pro Ala Gly Asp Lys Gly Asp Leu 20 25 30 Leu Leu Gln Ala Val Gln Gln Ser Cys Leu Ala Asp His Leu Leu Thr 35 40 45 Ala Ser Trp Gly Ala Asp Pro Val Pro Thr Lys Ala Pro Gly Glu Gly 50 55 60 Gln Glu Gly Leu Pro Leu Thr Gly Ala Cys Ala Gly Gly Pro Gly Leu 65 70 75 80 Pro Ala Gly Glu Leu Tyr Gly Trp Ala Val Glu Thr Thr Pro Ser Pro 85 90 95 Gly Pro Gln Pro Ala Ala Leu Thr Thr Gly Glu Ala Ala Ala Pro Glu 100 105 110 Ser Pro His Gln Ala Glu Pro Tyr Leu Ser Pro Ser Pro Ser Ala Cys 115 120 125 Thr Ala Val Gln Glu Pro Ser Pro Gly Ala Leu Asp Val Thr Ile Met 130 135 140 Tyr Lys Gly Arg Thr Val Leu Gln Lys Val Val Gly His Pro Ser Cys 145 150 155 160 Thr Phe Leu Tyr Gly Pro Pro Asp Pro Ala Val Arg Ala Thr Asp Pro 165 170 175 Gln Gln Val Ala Phe Pro Ser Pro Ala Glu Leu Pro Asp Gln Lys Gln 180 185 190 Leu Arg Tyr Thr Glu Glu Leu Leu Arg His Val Ala Pro Gly Leu His 195 200 205 Leu Glu Leu Arg Gly Pro Gln Leu Trp Ala Arg Arg Met Gly Lys Cys 210 215 220 Lys Val Tyr Trp Glu Val Gly Gly Pro Pro Gly Ser Ala Ser Pro Ser 225 230 235 240 Thr Pro Ala Cys Leu Leu Pro Arg Asn Cys Asp Thr Pro Ile Phe Asp 245 250 255 Phe Arg Val Phe Phe Gln Glu Leu Val Glu Phe Arg Ala Arg Gln Arg 260 265 270 Arg Gly Ser Pro Arg Tyr Thr Ile Tyr Leu Gly Phe Gly Gln Asp Leu 275 280 285 Ser Ala Gly Arg Pro Lys Glu Lys Ser Leu Val Leu Val Lys Leu Glu 290 295 300 Pro Trp Leu Cys Arg Val His Leu Glu Gly Thr Gln Arg Glu Gly Val 305 310 315 320 Ser Ser Leu Asp Ser Ser Ser Leu Ser Leu Cys Leu Ser Ser Ala Asn 325 330 335 Ser Leu Tyr Asp Asp Ile Glu Cys Phe Leu Met Glu Leu Glu Gln Pro 340 345 350 Ala < 210 > 21 < 211 > 92 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : 5 UTR < 400 > 21 tcaagctttt ggaccctcgt acagaagcta atacgactca ctatagggaa ataagagaga 60 aaagaagagt aagaagaaat ataagagcca cc 92 < 210 > 22 < 211 > 119 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : 3 UTR < 400 > 22 tgataatagg ctggagcctc ggtggccatg cttcttgccc cttgggcctc cccccagccc 60 ctcctcccct tcctgcaccc gtacccccgt ggtctttgaa taaagtctga gtgggcggc 119 < 210 > 23 < 211 > 164 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : 3 UTR with mi - 122 and mi - 142 . 3 p sites < 400 > 23 tgataatagg ctggagcctc ggtggccatg cttcttgccc cttgggccca aacaccattg 60 tcacactcca tccccccagc ccctcctccc cttcctccat aaagtaggaa acactacatg 120 cacccgtacc cccgtggtct ttgaataaag tctgagtggg cggc 164 < 210 > 24 < 211 > 22 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : 2 A peptide amino acid sequence < 400 > 24 Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 1 5 10 15 Glu Glu Asn Pro Gly Pro 20 < 210 > 25 < 211 > 66 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Nucleotide sequence encoding 2 A peptide < 400 > 25 ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct 60 ggacct 66 < 210 > 26 < 211 > 108 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Nucleotide sequence encoding 2 A peptide < 400 > 26 tccggactca gatccgggga tctcaaaatt gtcgctcctg tcaaacaaac tcttaacttt 60 gatttactca aactggctgg ggatgtagaa agcaatccag gtccactc 108 < 210 > 27 < 211 > 87 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : miR - 142 < 400 > 27 gacagugcag ucacccauaa aguagaaagc acuacuaaca gcacuggagg guguaguguu 60 uccuacuuua uggaugagug uacugug 87 < 210 > 28 < 211 > 23 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : miR - 142 - 3 p < 400 > 28 uguaguguuu ccuacuuuau gga 23 < 210 > 29 < 211 > 23 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : miR - 142 - 3 p binding site < 400 > 29 uccauaaagu aggaaacacu aca 23 < 210 > 30 < 211 > 21 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : miR - 142 - 5 p < 400 > 30 cauaaaguag aaagcacuac u 21 < 210 > 31 < 211 > 21 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : miR - 142 - 5 p binding site < 400 > 31 aguagugcuu ucuacuuuau g 21 < 210 > 32 < 211 > 22 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : miR - 122 - 3 p < 400 > 32 aacgccauua ucacacuaaa ua 22 < 210 > 33 < 211 > 22 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : miR - 122 - 5 p < 400 > 33 uggaguguga caaugguguu ug 22 < 210 > 34 < 211 > 22 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : miR - 21 - 5 p < 400 > 34 uagcuuauca gacugauguu ga 22 < 210 > 35 < 211 > 21 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : miR - 21 - 3 p < 400 > 35 caacaccagu cgaugggcug u 21 < 210 > 36 < 211 > 16 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 15 mer < 400 > 36 Met Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu 1 5 10 15 < 210 > 37 < 211 > 16 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 V ) 15 mer < 400 > 37 Met Lys Leu Val Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu 1 5 10 15 < 210 > 38 < 211 > 16 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 13 D ) 15 mer < 400 > 38 Met Leu Val Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr 1 5 10 15 < 210 > 39 < 211 > 25 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer < 400 > 39 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln 20 25 < 210 > 40 < 211 > 25 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 V ) 25 mer < 400 > 40 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Val Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln 20 25 < 210 > 41 < 211 > 25 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 13 D ) 25 mer < 400 > 41 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Asp Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln 20 25 < 210 > 42 < 211 > 46 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 15 mer ^ 3 < 400 > 42 Met Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu 1 5 10 15 Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu Lys 20 25 30 Leu Val Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu 35 40 45 < 210 > 43 < 211 > 46 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 V ) 15 mer ^ 3 < 400 > 43 Met Lys Leu Val Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu 1 5 10 15 Lys Leu Val Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu Lys 20 25 30 Leu Val Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu 35 40 45 < 210 > 44 < 211 > 46 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 13 D ) 15 mer ^ 3 < 400 > 44 Met Leu Val Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr 1 5 10 15 Leu Val Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr Leu 20 25 30 Val Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr 35 40 45 < 210 > 45 < 211 > 75 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer ^ 3 < 400 > 45 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln Met Thr Glu Tyr Lys Leu Val 20 25 30 Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu Thr Ile Gln Leu 35 40 45 Ile Gln Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Asp Gly Val 50 55 60 Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln 65 70 75 < 210 > 46 < 211 > 75 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 V ) 25 mer ^ 3 < 400 > 46 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Val Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln Met Thr Glu Tyr Lys Leu Val 20 25 30 Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu Thr Ile Gln Leu 35 40 45 Ile Gln Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Val Gly Val 50 55 60 Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln 65 70 75 < 210 > 47 < 211 > 75 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 13 D ) 25 mer ^ 3 < 400 > 47 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Asp Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln Met Thr Glu Tyr Lys Leu Val 20 25 30 Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr Ile Gln Leu 35 40 45 Ile Gln Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Asp Val 50 55 60 Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln 65 70 75 < 210 > 48 < 211 > 414 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 15 mer _ nt . STING ( V 155 M ) < 400 > 48 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser Ala Thr Asn Phe Ser 370 375 380 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Lys 385 390 395 400 Leu Val Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu 405 410 < 210 > 49 < 211 > 414 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 V ) 15 mer _ nt . STING ( V 155 M < 400 > 49 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser Ala Thr Asn Phe Ser 370 375 380 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Lys 385 390 395 400 Leu Val Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu 405 410 < 210 > 50 < 211 > 414 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 13 D ) 15 mer _ nt . STING ( V 155 M < 400 > 50 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser Ala Thr Asn Phe Ser 370 375 380 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Leu 385 390 395 400 Val Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr 405 410 < 210 > 51 < 211 > 423 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer _ nt . STING ( V 155 M ) < 400 > 51 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser Ala Thr Asn Phe Ser 370 375 380 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Thr 385 390 395 400 Glu Tyr Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala 405 410 415 Leu Thr Ile Gln Leu Ile Gln 420 < 210 > 52 < 211 > 423 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 V ) 25 mer _ nt . STING ( V 155 M < 400 > 52 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser Ala Thr Asn Phe Ser 370 375 380 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Thr 385 390 395 400 Glu Tyr Lys Leu Val Val Val Gly Ala Val Gly Val Gly Lys Ser Ala 405 410 415 Leu Thr Ile Gln Leu Ile Gln 420 < 210 > 53 < 211 > 423 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 13 D ) 25 mer _ nt . STING ( V 155 M < 400 > 53 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser Ala Thr Asn Phe Ser 370 375 380 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Thr 385 390 395 400 Glu Tyr Lys Leu Val Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala 405 410 415 Leu Thr Ile Gln Leu Ile Gln 420 < 210 > 54 < 211 > 444 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 15 mer ^ 3 _ nt . STING ( V 155 M ) < 400 > 54 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser Ala Thr Asn Phe Ser 370 375 380 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Lys 385 390 395 400 Leu Val Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu Lys Leu 405 410 415 Val Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu Lys Leu Val 420 425 430 Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu 435 440 < 210 > 55 < 211 > 444 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 V ) 15 mer ^ 3 _ nt . STING ( V 155 M < 400 > 55 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser Ala Thr Asn Phe Ser 370 375 380 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Lys 385 390 395 400 Leu Val Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu Lys Leu 405 410 415 Val Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu Lys Leu Val 420 425 430 Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu 435 440 < 210 > 56 < 211 > 444 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 13 D ) 15 mer ^ 3 _ nt . STING ( V 155 M < 400 > 56 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser Ala Thr Asn Phe Ser 370 375 380 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Leu 385 390 395 400 Val Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr Leu Val 405 410 415 Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr Leu Val Val 420 425 430 Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr 435 440 < 210 > 57 < 211 > 473 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer ^ 3 _ nt . STING ( V 155 M ) < 400 > 57 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser Ala Thr Asn Phe Ser 370 375 380 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Thr 385 390 395 400 Glu Tyr Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala 405 410 415 Leu Thr Ile Gln Leu Ile Gln Met Thr Glu Tyr Lys Leu Val Val Val 420 425 430 Gly Ala Asp Gly Val Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln 435 440 445 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys 450 455 460 Ser Ala Leu Thr Ile Gln Leu Ile Gln 465 470 < 210 > 58 < 211 > 473 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 V ) 25 mer ^ 3 _ nt . STING ( V 155 M < 400 > 58 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser Ala Thr Asn Phe Ser 370 375 380 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Thr 385 390 395 400 Glu Tyr Lys Leu Val Val Val Gly Ala Val Gly Val Gly Lys Ser Ala 405 410 415 Leu Thr Ile Gln Leu Ile Gln Met Thr Glu Tyr Lys Leu Val Val Val 420 425 430 Gly Ala Val Gly Val Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln 435 440 445 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Val Gly Val Gly Lys 450 455 460 Ser Ala Leu Thr Ile Gln Leu Ile Gln 465 470 < 210 > 59 < 211 > 473 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 13 D ) 25 mer ^ 3 _ nt . STING ( V 155 M < 400 > 59 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser Ala Thr Asn Phe Ser 370 375 380 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Thr 385 390 395 400 Glu Tyr Lys Leu Val Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala 405 410 415 Leu Thr Ile Gln Leu Ile Gln Met Thr Glu Tyr Lys Leu Val Val Val 420 425 430 Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln 435 440 445 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Asp Val Gly Lys 450 455 460 Ser Ala Leu Thr Ile Gln Leu Ile Gln 465 470 < 210 > 60 < 211 > 414 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 15 mer _ ct . STING ( V 155 M ) < 400 > 60 Met Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu 1 5 10 15 Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 20 25 30 Pro Gly Pro Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro 35 40 45 Arg Gly His Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys 50 55 60 Leu Val Thr Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg 65 70 75 80 Tyr Leu Val Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn 85 90 95 Gly Val Cys Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr 100 105 110 Arg Gly Ser Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu 115 120 125 Arg Arg Gly Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu 130 135 140 Pro Asn Ala Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly 145 150 155 160 Leu Ser Gln Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro 165 170 175 Ala Glu Ile Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His 180 185 190 Gly Leu Ala Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro 195 200 205 Glu Leu Gln Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu 210 215 220 Leu Arg Gly Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp 225 230 235 240 Cys Gly Val Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe 245 250 255 Leu Asp Lys Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp 260 265 270 Arg Val Tyr Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg 275 280 285 Ala Gly Thr Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe 290 295 300 Ala Met Ser Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu 305 310 315 320 Glu Gln Ala Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp 325 330 335 Ala Pro Glu Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro 340 345 350 Ala Asp Asp Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu 355 360 365 Arg Gln Glu Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser 370 375 380 Ala Val Pro Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile 385 390 395 400 Ser Gly Met Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 405 410 < 210 > 61 < 211 > 414 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 V ) 15 mer _ ct . STING ( V 155 M < 400 > 61 Met Lys Leu Val Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu 1 5 10 15 Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 20 25 30 Pro Gly Pro Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro 35 40 45 Arg Gly His Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys 50 55 60 Leu Val Thr Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg 65 70 75 80 Tyr Leu Val Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn 85 90 95 Gly Val Cys Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr 100 105 110 Arg Gly Ser Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu 115 120 125 Arg Arg Gly Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu 130 135 140 Pro Asn Ala Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly 145 150 155 160 Leu Ser Gln Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro 165 170 175 Ala Glu Ile Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His 180 185 190 Gly Leu Ala Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro 195 200 205 Glu Leu Gln Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu 210 215 220 Leu Arg Gly Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp 225 230 235 240 Cys Gly Val Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe 245 250 255 Leu Asp Lys Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp 260 265 270 Arg Val Tyr Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg 275 280 285 Ala Gly Thr Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe 290 295 300 Ala Met Ser Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu 305 310 315 320 Glu Gln Ala Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp 325 330 335 Ala Pro Glu Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro 340 345 350 Ala Asp Asp Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu 355 360 365 Arg Gln Glu Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser 370 375 380 Ala Val Pro Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile 385 390 395 400 Ser Gly Met Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 405 410 < 210 > 62 < 211 > 414 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 13 D ) 15 mer _ ct . STING ( V 155 M < 400 > 62 Met Leu Val Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr 1 5 10 15 Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 20 25 30 Pro Gly Pro Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro 35 40 45 Arg Gly His Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys 50 55 60 Leu Val Thr Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg 65 70 75 80 Tyr Leu Val Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn 85 90 95 Gly Val Cys Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr 100 105 110 Arg Gly Ser Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu 115 120 125 Arg Arg Gly Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu 130 135 140 Pro Asn Ala Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly 145 150 155 160 Leu Ser Gln Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro 165 170 175 Ala Glu Ile Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His 180 185 190 Gly Leu Ala Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro 195 200 205 Glu Leu Gln Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu 210 215 220 Leu Arg Gly Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp 225 230 235 240 Cys Gly Val Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe 245 250 255 Leu Asp Lys Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp 260 265 270 Arg Val Tyr Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg 275 280 285 Ala Gly Thr Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe 290 295 300 Ala Met Ser Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu 305 310 315 320 Glu Gln Ala Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp 325 330 335 Ala Pro Glu Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro 340 345 350 Ala Asp Asp Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu 355 360 365 Arg Gln Glu Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser 370 375 380 Ala Val Pro Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile 385 390 395 400 Ser Gly Met Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 405 410 < 210 > 63 < 211 > 423 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer _ ct . STING ( V 155 M ) < 400 > 63 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln Ala Thr Asn Phe Ser Leu Leu 20 25 30 Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Pro His Ser 35 40 45 Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His Gly Ala Gln Lys 50 55 60 Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr Leu Trp Gly Leu 65 70 75 80 Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val Leu His Leu Ala 85 90 95 Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys Ser Leu Ala Glu 100 105 110 Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser Tyr Trp Arg Thr 115 120 125 Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly Ala Leu Leu Leu 130 135 140 Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala Val Gly Pro Pro 145 150 155 160 Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln Ala Leu Asn Ile 165 170 175 Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile Ser Ala Val Cys 180 185 190 Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala Trp Ser Tyr Tyr 195 200 205 Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln Ala Arg Ile Arg 210 215 220 Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly Ala Val Ser Gln 225 230 235 240 Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val Pro Asp Asn Leu 245 250 255 Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys Leu Pro Gln Gln 260 265 270 Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr Ser Asn Ser Ile 275 280 285 Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr Cys Val Leu Glu 290 295 300 Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser Gln Tyr Ser Gln 305 310 315 320 Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala Lys Leu Phe Cys 325 330 335 Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu Ser Gln Asn Asn 340 345 350 Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp Ser Ser Phe Ser 355 360 365 Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu Glu Lys Glu Glu 370 375 380 Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro Ser Thr Ser Thr 385 390 395 400 Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met Glu Lys Pro Leu 405 410 415 Pro Leu Arg Thr Asp Phe Ser 420 < 210 > 64 < 211 > 423 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 V ) 25 mer _ ct . STING ( V 155 M < 400 > 64 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Val Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln Ala Thr Asn Phe Ser Leu Leu 20 25 30 Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Pro His Ser 35 40 45 Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His Gly Ala Gln Lys 50 55 60 Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr Leu Trp Gly Leu 65 70 75 80 Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val Leu His Leu Ala 85 90 95 Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys Ser Leu Ala Glu 100 105 110 Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser Tyr Trp Arg Thr 115 120 125 Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly Ala Leu Leu Leu 130 135 140 Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala Val Gly Pro Pro 145 150 155 160 Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln Ala Leu Asn Ile 165 170 175 Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile Ser Ala Val Cys 180 185 190 Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala Trp Ser Tyr Tyr 195 200 205 Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln Ala Arg Ile Arg 210 215 220 Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly Ala Val Ser Gln 225 230 235 240 Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val Pro Asp Asn Leu 245 250 255 Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys Leu Pro Gln Gln 260 265 270 Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr Ser Asn Ser Ile 275 280 285 Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr Cys Val Leu Glu 290 295 300 Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser Gln Tyr Ser Gln 305 310 315 320 Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala Lys Leu Phe Cys 325 330 335 Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu Ser Gln Asn Asn 340 345 350 Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp Ser Ser Phe Ser 355 360 365 Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu Glu Lys Glu Glu 370 375 380 Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro Ser Thr Ser Thr 385 390 395 400 Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met Glu Lys Pro Leu 405 410 415 Pro Leu Arg Thr Asp Phe Ser 420 < 210 > 65 < 211 > 423 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 13 D ) 25 mer _ ct . STING ( V 155 M < 400 > 65 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Asp Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln Ala Thr Asn Phe Ser Leu Leu 20 25 30 Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Pro His Ser 35 40 45 Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His Gly Ala Gln Lys 50 55 60 Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr Leu Trp Gly Leu 65 70 75 80 Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val Leu His Leu Ala 85 90 95 Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys Ser Leu Ala Glu 100 105 110 Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser Tyr Trp Arg Thr 115 120 125 Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly Ala Leu Leu Leu 130 135 140 Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala Val Gly Pro Pro 145 150 155 160 Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln Ala Leu Asn Ile 165 170 175 Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile Ser Ala Val Cys 180 185 190 Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala Trp Ser Tyr Tyr 195 200 205 Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln Ala Arg Ile Arg 210 215 220 Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly Ala Val Ser Gln 225 230 235 240 Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val Pro Asp Asn Leu 245 250 255 Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys Leu Pro Gln Gln 260 265 270 Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr Ser Asn Ser Ile 275 280 285 Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr Cys Val Leu Glu 290 295 300 Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser Gln Tyr Ser Gln 305 310 315 320 Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala Lys Leu Phe Cys 325 330 335 Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu Ser Gln Asn Asn 340 345 350 Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp Ser Ser Phe Ser 355 360 365 Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu Glu Lys Glu Glu 370 375 380 Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro Ser Thr Ser Thr 385 390 395 400 Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met Glu Lys Pro Leu 405 410 415 Pro Leu Arg Thr Asp Phe Ser 420 < 210 > 66 < 211 > 444 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 15 mer ^ 3 _ ct . STING ( V 155 M ) < 400 > 66 Met Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu 1 5 10 15 Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu Lys 20 25 30 Leu Val Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu Ala Thr 35 40 45 Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 50 55 60 Pro Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly 65 70 75 80 His Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val 85 90 95 Thr Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu 100 105 110 Val Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val 115 120 125 Cys Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly 130 135 140 Ser Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg 145 150 155 160 Gly Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn 165 170 175 Ala Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser 180 185 190 Gln Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu 195 200 205 Ile Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu 210 215 220 Ala Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu 225 230 235 240 Gln Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg 245 250 255 Gly Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly 260 265 270 Val Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp 275 280 285 Lys Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val 290 295 300 Tyr Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly 305 310 315 320 Thr Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met 325 330 335 Ser Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln 340 345 350 Ala Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro 355 360 365 Glu Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp 370 375 380 Asp Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln 385 390 395 400 Glu Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val 405 410 415 Pro Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly 420 425 430 Met Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 435 440 < 210 > 67 < 211 > 444 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 V ) 15 mer ^ 3 _ ct . STING ( V 155 M < 400 > 67 Met Lys Leu Val Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu 1 5 10 15 Lys Leu Val Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu Lys 20 25 30 Leu Val Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu Ala Thr 35 40 45 Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 50 55 60 Pro Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly 65 70 75 80 His Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val 85 90 95 Thr Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu 100 105 110 Val Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val 115 120 125 Cys Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly 130 135 140 Ser Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg 145 150 155 160 Gly Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn 165 170 175 Ala Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser 180 185 190 Gln Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu 195 200 205 Ile Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu 210 215 220 Ala Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu 225 230 235 240 Gln Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg 245 250 255 Gly Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly 260 265 270 Val Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp 275 280 285 Lys Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val 290 295 300 Tyr Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly 305 310 315 320 Thr Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met 325 330 335 Ser Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln 340 345 350 Ala Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro 355 360 365 Glu Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp 370 375 380 Asp Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln 385 390 395 400 Glu Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val 405 410 415 Pro Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly 420 425 430 Met Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 435 440 < 210 > 68 < 211 > 444 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 13 D ) 15 mer ^ 3 _ ct . STING ( V 155 M < 400 > 68 Met Leu Val Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr 1 5 10 15 Leu Val Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr Leu 20 25 30 Val Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr Ala Thr 35 40 45 Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 50 55 60 Pro Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly 65 70 75 80 His Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val 85 90 95 Thr Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu 100 105 110 Val Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val 115 120 125 Cys Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly 130 135 140 Ser Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg 145 150 155 160 Gly Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn 165 170 175 Ala Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser 180 185 190 Gln Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu 195 200 205 Ile Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu 210 215 220 Ala Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu 225 230 235 240 Gln Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg 245 250 255 Gly Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly 260 265 270 Val Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp 275 280 285 Lys Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val 290 295 300 Tyr Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly 305 310 315 320 Thr Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met 325 330 335 Ser Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln 340 345 350 Ala Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro 355 360 365 Glu Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp 370 375 380 Asp Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln 385 390 395 400 Glu Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val 405 410 415 Pro Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly 420 425 430 Met Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser 435 440 < 210 > 69 < 211 > 473 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer ^ 3 _ ct . STING ( V 155 M ) < 400 > 69 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln Met Thr Glu Tyr Lys Leu Val 20 25 30 Val Val Gly Ala Asp Gly Val Gly Lys Ser Ala Leu Thr Ile Gln Leu 35 40 45 Ile Gln Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Asp Gly Val 50 55 60 Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln Ala Thr Asn Phe Ser 65 70 75 80 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Pro 85 90 95 His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His Gly Ala 100 105 110 Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr Leu Trp 115 120 125 Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val Leu His 130 135 140 Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys Ser Leu 145 150 155 160 Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser Tyr Trp 165 170 175 Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly Ala Leu 180 185 190 Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala Val Gly 195 200 205 Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln Ala Leu 210 215 220 Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile Ser Ala 225 230 235 240 Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala Trp Ser 245 250 255 Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln Ala Arg 260 265 270 Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly Ala Val 275 280 285 Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val Pro Asp 290 295 300 Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys Leu Pro 305 310 315 320 Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr Ser Asn 325 330 335 Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr Cys Val 340 345 350 Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser Gln Tyr 355 360 365 Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala Lys Leu 370 375 380 Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu Ser Gln 385 390 395 400 Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp Ser Ser 405 410 415 Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu Glu Lys 420 425 430 Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro Ser Thr 435 440 445 Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met Glu Lys 450 455 460 Pro Leu Pro Leu Arg Thr Asp Phe Ser 465 470 < 210 > 70 < 211 > 473 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 V ) 25 mer ^ 3 _ ct . STING ( V 155 M < 400 > 70 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Val Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln Met Thr Glu Tyr Lys Leu Val 20 25 30 Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu Thr Ile Gln Leu 35 40 45 Ile Gln Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Val Gly Val 50 55 60 Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln Ala Thr Asn Phe Ser 65 70 75 80 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Pro 85 90 95 His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His Gly Ala 100 105 110 Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr Leu Trp 115 120 125 Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val Leu His 130 135 140 Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys Ser Leu 145 150 155 160 Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser Tyr Trp 165 170 175 Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly Ala Leu 180 185 190 Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala Val Gly 195 200 205 Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln Ala Leu 210 215 220 Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile Ser Ala 225 230 235 240 Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala Trp Ser 245 250 255 Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln Ala Arg 260 265 270 Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly Ala Val 275 280 285 Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val Pro Asp 290 295 300 Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys Leu Pro 305 310 315 320 Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr Ser Asn 325 330 335 Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr Cys Val 340 345 350 Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser Gln Tyr 355 360 365 Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala Lys Leu 370 375 380 Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu Ser Gln 385 390 395 400 Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp Ser Ser 405 410 415 Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu Glu Lys 420 425 430 Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro Ser Thr 435 440 445 Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met Glu Lys 450 455 460 Pro Leu Pro Leu Arg Thr Asp Phe Ser 465 470 < 210 > 71 < 211 > 473 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 13 D ) 25 mer ^ 3 _ ct . STING ( V 155 M < 400 > 71 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Asp Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln Met Thr Glu Tyr Lys Leu Val 20 25 30 Val Val Gly Ala Gly Asp Val Gly Lys Ser Ala Leu Thr Ile Gln Leu 35 40 45 Ile Gln Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Asp Val 50 55 60 Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln Ala Thr Asn Phe Ser 65 70 75 80 Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Pro 85 90 95 His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His Gly Ala 100 105 110 Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr Leu Trp 115 120 125 Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val Leu His 130 135 140 Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys Ser Leu 145 150 155 160 Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser Tyr Trp 165 170 175 Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly Ala Leu 180 185 190 Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala Val Gly 195 200 205 Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln Ala Leu 210 215 220 Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile Ser Ala 225 230 235 240 Val Cys Glu Lys Gly Asn Phe Asn Met Ala His Gly Leu Ala Trp Ser 245 250 255 Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln Ala Arg 260 265 270 Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly Ala Val 275 280 285 Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val Pro Asp 290 295 300 Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys Leu Pro 305 310 315 320 Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr Ser Asn 325 330 335 Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr Cys Val 340 345 350 Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser Gln Tyr 355 360 365 Ser Gln Ala Gly Phe Ser Arg Glu Asp Arg Leu Glu Gln Ala Lys Leu 370 375 380 Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu Ser Gln 385 390 395 400 Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp Ser Ser 405 410 415 Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu Glu Lys 420 425 430 Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro Ser Thr 435 440 445 Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met Glu Lys 450 455 460 Pro Leu Pro Leu Arg Thr Asp Phe Ser 465 470 < 210 > 72 < 211 > 25 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 C ) 25 mer < 400 > 72 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Cys Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln 20 25 < 210 > 73 < 211 > 75 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 C ) 25 mer ^ 3 < 400 > 73 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Cys Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln Met Thr Glu Tyr Lys Leu Val 20 25 30 Val Val Gly Ala Cys Gly Val Gly Lys Ser Ala Leu Thr Ile Gln Leu 35 40 45 Ile Gln Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Cys Gly Val 50 55 60 Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln 65 70 75 < 210 > 74 < 211 > 25 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( WT ) 25 mer < 400 > 74 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln 20 25 < 210 > 75 < 211 > 296 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 296 ) < 223 > human myd 88 ( L 265 P ) ; P 4027 without epitope tag < 400 > 75 Met Ser Ala Gly Asp Pro Arg Val Gly Ser Gly Ser Leu Asp Ser Phe 1 5 10 15 Met Phe Ser Ile Pro Leu Val Ala Leu Asn Val Gly Val Arg Arg Arg 20 25 30 Leu Ser Leu Phe Leu Asn Pro Arg Thr Pro Val Ala Ala Asp Trp Thr 35 40 45 Leu Leu Ala Glu Glu Met Gly Phe Glu Tyr Leu Glu Ile Arg Glu Leu 50 55 60 Glu Thr Arg Pro Asp Pro Thr Arg Ser Leu Leu Asp Ala Trp Gln Gly 65 70 75 80 Arg Ser Gly Ala Ser Val Gly Arg Leu Leu Glu Leu Leu Ala Leu Leu 85 90 95 Asp Arg Glu Asp Ile Leu Lys Glu Leu Lys Ser Arg Ile Glu Glu Asp 100 105 110 Cys Gln Lys Tyr Leu Gly Lys Gln Gln Asn Gln Glu Ser Glu Lys Pro 115 120 125 Leu Gln Val Ala Arg Val Glu Ser Ser Val Pro Gln Thr Lys Glu Leu 130 135 140 Gly Gly Ile Thr Thr Leu Asp Asp Pro Leu Gly Gln Thr Pro Glu Leu 145 150 155 160 Phe Asp Ala Phe Ile Cys Tyr Cys Pro Asn Asp Ile Glu Phe Val Gln 165 170 175 Glu Met Ile Arg Gln Leu Glu Gln Thr Asp Tyr Arg Leu Lys Leu Cys 180 185 190 Val Ser Asp Arg Asp Val Leu Pro Gly Thr Cys Val Trp Ser Ile Ala 195 200 205 Ser Glu Leu Ile Glu Lys Arg Cys Arg Arg Met Val Val Val Val Ser 210 215 220 Asp Asp Tyr Leu Gln Ser Lys Glu Cys Asp Phe Gln Thr Lys Phe Ala 225 230 235 240 Leu Ser Leu Ser Pro Gly Val Gln Gln Lys Arg Pro Ile Pro Ile Lys 245 250 255 Tyr Lys Ala Met Lys Lys Asp Phe Pro Ser Ile Leu Arg Phe Ile Thr 260 265 270 Ile Cys Asp Tyr Thr Asn Pro Cys Thr Lys Ser Trp Phe Trp Thr Arg 275 280 285 Leu Ala Lys Ala Leu Ser Leu Pro 290 295 < 210 > 76 < 211 > 296 < 212 > PRT < 213 > Mus musculus < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 296 ) < 223 > mouse myd 88 ( L 265 P ) ; P 4028 without epitope tag < 400 > 76 Met Ala Ala Gly Gly Pro Gly Ala Gly Ser Ala Ala Pro Val Ser Ser 1 5 10 15 Thr Ser Ser Leu Pro Leu Ala Ala Leu Asn Met Arg Val Arg Arg Arg 20 25 30 Leu Ser Leu Phe Leu Asn Val Arg Thr Gln Val Ala Ala Asp Trp Thr 35 40 45 Ala Leu Ala Glu Glu Met Asp Phe Glu Tyr Leu Glu Ile Arg Gln Leu 50 55 60 Glu Thr Gln Ala Asp Pro Thr Gly Arg Leu Leu Asp Ala Trp Gln Gly 65 70 75 80 Arg Pro Gly Ala Ser Val Gly Arg Leu Leu Glu Leu Leu Thr Lys Leu 85 90 95 Gly Arg Asp Asp Val Leu Leu Glu Leu Gly Pro Ser Ile Glu Glu Asp 100 105 110 Cys Gln Lys Tyr Ile Leu Lys Gln Gln Gln Glu Glu Ala Glu Lys Pro 115 120 125 Leu Gln Val Ala Ala Val Asp Ser Ser Val Pro Arg Thr Ala Glu Leu 130 135 140 Ala Gly Ile Thr Thr Leu Asp Asp Pro Leu Gly His Met Pro Glu Arg 145 150 155 160 Phe Asp Ala Phe Ile Cys Tyr Cys Pro Ser Asp Ile Gln Phe Val Gln 165 170 175 Glu Met Ile Arg Gln Leu Glu Gln Thr Asn Tyr Arg Leu Lys Leu Cys 180 185 190 Val Ser Asp Arg Asp Val Leu Pro Gly Thr Cys Val Trp Ser Ile Ala 195 200 205 Ser Glu Leu Ile Glu Lys Arg Cys Arg Arg Met Val Val Val Val Ser 210 215 220 Asp Asp Tyr Leu Gln Ser Lys Glu Cys Asp Phe Gln Thr Lys Phe Ala 225 230 235 240 Leu Ser Leu Ser Pro Gly Ala His Gln Lys Arg Pro Ile Pro Ile Lys 245 250 255 Tyr Lys Ala Met Lys Lys Glu Phe Pro Ser Ile Leu Arg Phe Ile Thr 260 265 270 Val Cys Asp Tyr Thr Asn Pro Cys Thr Lys Ser Trp Phe Trp Thr Arg 275 280 285 Leu Ala Lys Ala Leu Ser Leu Pro 290 295 < 210 > 77 < 211 > 232 < 212 > PRT < 213 > Mus musculus < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 232 ) < 223 > Mouse TRAM ( TICAM 2 ) ; P 4033 without epitope tag < 400 > 77 Met Gly Val Gly Lys Ser Lys Leu Asp Lys Cys Pro Leu Ser Trp His 1 5 10 15 Lys Lys Asp Ser Val Asp Ala Asp Gln Asp Gly His Glu Ser Asp Ser 20 25 30 Lys Asn Ser Glu Glu Ala Cys Leu Arg Gly Phe Val Glu Gln Ser Ser 35 40 45 Gly Ser Glu Pro Pro Thr Gly Glu Gln Asp Gln Pro Glu Ala Lys Gly 50 55 60 Ala Gly Pro Glu Glu Gln Asp Glu Glu Glu Phe Leu Lys Phe Val Ile 65 70 75 80 Leu His Ala Glu Asp Asp Thr Asp Glu Ala Leu Arg Val Gln Asp Leu 85 90 95 Leu Gln Asn Asp Phe Gly Ile Arg Pro Gly Ile Val Phe Ala Glu Met 100 105 110 Pro Cys Gly Arg Leu His Leu Gln Asn Leu Asp Asp Ala Val Asn Gly 115 120 125 Ser Ala Trp Thr Ile Leu Leu Leu Thr Glu Asn Phe Leu Arg Asp Thr 130 135 140 Trp Cys Asn Phe Gln Phe Tyr Thr Ser Leu Met Asn Ser Val Ser Arg 145 150 155 160 Gln His Lys Tyr Asn Ser Val Ile Pro Met Arg Pro Leu Asn Ser Pro 165 170 175 Leu Pro Arg Glu Arg Thr Pro Leu Ala Leu Gln Thr Ile Asn Ala Leu 180 185 190 Glu Glu Glu Ser Gln Gly Phe Ser Thr Gln Val Glu Arg Ile Phe Arg 195 200 205 Glu Ser Val Phe Glu Arg Gln Gln Ser Ile Trp Lys Glu Thr Arg Ser 210 215 220 Val Ser Gln Lys Gln Phe Ile Ala 225 230 < 210 > 78 < 211 > 847 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : STAT 6 V 547 A / T 548 A ) ; P 008 with no epitope tag < 400 > 78 Met Ser Leu Trp Gly Leu Val Ser Lys Met Pro Pro Glu Lys Val Gln 1 5 10 15 Arg Leu Tyr Val Asp Phe Pro Gln His Leu Arg His Leu Leu Gly Asp 20 25 30 Trp Leu Glu Ser Gln Pro Trp Glu Phe Leu Val Gly Ser Asp Ala Phe 35 40 45 Cys Cys Asn Leu Ala Ser Ala Leu Leu Ser Asp Thr Val Gln His Leu 50 55 60 Gln Ala Ser Val Gly Glu Gln Gly Glu Gly Ser Thr Ile Leu Gln His 65 70 75 80 Ile Ser Thr Leu Glu Ser Ile Tyr Gln Arg Asp Pro Leu Lys Leu Val 85 90 95 Ala Thr Phe Arg Gln Ile Leu Gln Gly Glu Lys Lys Ala Val Met Glu 100 105 110 Gln Phe Arg His Leu Pro Met Pro Phe His Trp Lys Gln Glu Glu Leu 115 120 125 Lys Phe Lys Thr Gly Leu Arg Arg Leu Gln His Arg Val Gly Glu Ile 130 135 140 His Leu Leu Arg Glu Ala Leu Gln Lys Gly Ala Glu Ala Gly Gln Val 145 150 155 160 Ser Leu His Ser Leu Ile Glu Thr Pro Ala Asn Gly Thr Gly Pro Ser 165 170 175 Glu Ala Leu Ala Met Leu Leu Gln Glu Thr Thr Gly Glu Leu Glu Ala 180 185 190 Ala Lys Ala Leu Val Leu Lys Arg Ile Gln Ile Trp Lys Arg Gln Gln 195 200 205 Gln Leu Ala Gly Asn Gly Ala Pro Phe Glu Glu Ser Leu Ala Pro Leu 210 215 220 Gln Glu Arg Cys Glu Ser Leu Val Asp Ile Tyr Ser Gln Leu Gln Gln 225 230 235 240 Glu Val Gly Ala Ala Gly Gly Glu Leu Glu Pro Lys Thr Arg Ala Ser 245 250 255 Leu Thr Gly Arg Leu Asp Glu Val Leu Arg Thr Leu Val Thr Ser Cys 260 265 270 Phe Leu Val Glu Lys Gln Pro Pro Gln Val Leu Lys Thr Gln Thr Lys 275 280 285 Phe Gln Ala Gly Val Arg Phe Leu Leu Gly Leu Arg Phe Leu Gly Ala 290 295 300 Pro Ala Lys Pro Pro Leu Val Arg Ala Asp Met Val Thr Glu Lys Gln 305 310 315 320 Ala Arg Glu Leu Ser Val Pro Gln Gly Pro Gly Ala Gly Ala Glu Ser 325 330 335 Thr Gly Glu Ile Ile Asn Asn Thr Val Pro Leu Glu Asn Ser Ile Pro 340 345 350 Gly Asn Cys Cys Ser Ala Leu Phe Lys Asn Leu Leu Leu Lys Lys Ile 355 360 365 Lys Arg Cys Glu Arg Lys Gly Thr Glu Ser Val Thr Glu Glu Lys Cys 370 375 380 Ala Val Leu Phe Ser Ala Ser Phe Thr Leu Gly Pro Gly Lys Leu Pro 385 390 395 400 Ile Gln Leu Gln Ala Leu Ser Leu Pro Leu Val Val Ile Val His Gly 405 410 415 Asn Gln Asp Asn Asn Ala Lys Ala Thr Ile Leu Trp Asp Asn Ala Phe 420 425 430 Ser Glu Met Asp Arg Val Pro Phe Val Val Ala Glu Arg Val Pro Trp 435 440 445 Glu Lys Met Cys Glu Thr Leu Asn Leu Lys Phe Met Ala Glu Val Gly 450 455 460 Thr Asn Arg Gly Leu Leu Pro Glu His Phe Leu Phe Leu Ala Gln Lys 465 470 475 480 Ile Phe Asn Asp Asn Ser Leu Ser Met Glu Ala Phe Gln His Arg Ser 485 490 495 Val Ser Trp Ser Gln Phe Asn Lys Glu Ile Leu Leu Gly Arg Gly Phe 500 505 510 Thr Phe Trp Gln Trp Phe Asp Gly Val Leu Asp Leu Thr Lys Arg Cys 515 520 525 Leu Arg Ser Tyr Trp Ser Asp Arg Leu Ile Ile Gly Phe Ile Ser Lys 530 535 540 Gln Tyr Ala Ala Ser Leu Leu Leu Asn Glu Pro Asp Gly Thr Phe Leu 545 550 555 560 Leu Arg Phe Ser Asp Ser Glu Ile Gly Gly Ile Thr Ile Ala His Val 565 570 575 Ile Arg Gly Gln Asp Gly Ser Pro Gln Ile Glu Asn Ile Gln Pro Phe 580 585 590 Ser Ala Lys Asp Leu Ser Ile Arg Ser Leu Gly Asp Arg Ile Arg Asp 595 600 605 Leu Ala Gln Leu Lys Asn Leu Tyr Pro Lys Lys Pro Lys Asp Glu Ala 610 615 620 Phe Arg Ser His Tyr Lys Pro Glu Gln Met Gly Lys Asp Gly Arg Gly 625 630 635 640 Tyr Val Pro Ala Thr Ile Lys Met Thr Val Glu Arg Asp Gln Pro Leu 645 650 655 Pro Thr Pro Glu Leu Gln Met Pro Thr Met Val Pro Ser Tyr Asp Leu 660 665 670 Gly Met Ala Pro Asp Ser Ser Met Ser Met Gln Leu Gly Pro Asp Met 675 680 685 Val Pro Gln Val Tyr Pro Pro His Ser His Ser Ile Pro Pro Tyr Gln 690 695 700 Gly Leu Ser Pro Glu Glu Ser Val Asn Val Leu Ser Ala Phe Gln Glu 705 710 715 720 Pro His Leu Gln Met Pro Pro Ser Leu Gly Gln Met Ser Leu Pro Phe 725 730 735 Asp Gln Pro His Pro Gln Gly Leu Leu Pro Cys Gln Pro Gln Glu His 740 745 750 Ala Val Ser Ser Pro Asp Pro Leu Leu Cys Ser Asp Val Thr Met Val 755 760 765 Glu Asp Ser Cys Leu Ser Gln Pro Val Thr Ala Phe Pro Gln Gly Thr 770 775 780 Trp Ile Gly Glu Asp Ile Phe Pro Pro Leu Leu Pro Pro Thr Glu Gln 785 790 795 800 Asp Leu Thr Lys Leu Leu Leu Glu Gly Gln Gly Glu Ser Gly Gly Gly 805 810 815 Ser Leu Gly Ala Gln Pro Leu Leu Gln Pro Ser His Tyr Gly Gln Ser 820 825 830 Gly Ile Ser Met Ser His Met Asp Leu Arg Ala Asn Pro Ser Trp 835 840 845 < 210 > 79 < 211 > 847 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : STAT 6 ( S 407 D ) ; P 009 with no epitope tag < 400 > 79 Met Ser Leu Trp Gly Leu Val Ser Lys Met Pro Pro Glu Lys Val Gln 1 5 10 15 Arg Leu Tyr Val Asp Phe Pro Gln His Leu Arg His Leu Leu Gly Asp 20 25 30 Trp Leu Glu Ser Gln Pro Trp Glu Phe Leu Val Gly Ser Asp Ala Phe 35 40 45 Cys Cys Asn Leu Ala Ser Ala Leu Leu Ser Asp Thr Val Gln His Leu 50 55 60 Gln Ala Ser Val Gly Glu Gln Gly Glu Gly Ser Thr Ile Leu Gln His 65 70 75 80 Ile Ser Thr Leu Glu Ser Ile Tyr Gln Arg Asp Pro Leu Lys Leu Val 85 90 95 Ala Thr Phe Arg Gln Ile Leu Gln Gly Glu Lys Lys Ala Val Met Glu 100 105 110 Gln Phe Arg His Leu Pro Met Pro Phe His Trp Lys Gln Glu Glu Leu 115 120 125 Lys Phe Lys Thr Gly Leu Arg Arg Leu Gln His Arg Val Gly Glu Ile 130 135 140 His Leu Leu Arg Glu Ala Leu Gln Lys Gly Ala Glu Ala Gly Gln Val 145 150 155 160 Ser Leu His Ser Leu Ile Glu Thr Pro Ala Asn Gly Thr Gly Pro Ser 165 170 175 Glu Ala Leu Ala Met Leu Leu Gln Glu Thr Thr Gly Glu Leu Glu Ala 180 185 190 Ala Lys Ala Leu Val Leu Lys Arg Ile Gln Ile Trp Lys Arg Gln Gln 195 200 205 Gln Leu Ala Gly Asn Gly Ala Pro Phe Glu Glu Ser Leu Ala Pro Leu 210 215 220 Gln Glu Arg Cys Glu Ser Leu Val Asp Ile Tyr Ser Gln Leu Gln Gln 225 230 235 240 Glu Val Gly Ala Ala Gly Gly Glu Leu Glu Pro Lys Thr Arg Ala Ser 245 250 255 Leu Thr Gly Arg Leu Asp Glu Val Leu Arg Thr Leu Val Thr Ser Cys 260 265 270 Phe Leu Val Glu Lys Gln Pro Pro Gln Val Leu Lys Thr Gln Thr Lys 275 280 285 Phe Gln Ala Gly Val Arg Phe Leu Leu Gly Leu Arg Phe Leu Gly Ala 290 295 300 Pro Ala Lys Pro Pro Leu Val Arg Ala Asp Met Val Thr Glu Lys Gln 305 310 315 320 Ala Arg Glu Leu Ser Val Pro Gln Gly Pro Gly Ala Gly Ala Glu Ser 325 330 335 Thr Gly Glu Ile Ile Asn Asn Thr Val Pro Leu Glu Asn Ser Ile Pro 340 345 350 Gly Asn Cys Cys Ser Ala Leu Phe Lys Asn Leu Leu Leu Lys Lys Ile 355 360 365 Lys Arg Cys Glu Arg Lys Gly Thr Glu Ser Val Thr Glu Glu Lys Cys 370 375 380 Ala Val Leu Phe Ser Ala Ser Phe Thr Leu Gly Pro Gly Lys Leu Pro 385 390 395 400 Ile Gln Leu Gln Ala Leu Asp Leu Pro Leu Val Val Ile Val His Gly 405 410 415 Asn Gln Asp Asn Asn Ala Lys Ala Thr Ile Leu Trp Asp Asn Ala Phe 420 425 430 Ser Glu Met Asp Arg Val Pro Phe Val Val Ala Glu Arg Val Pro Trp 435 440 445 Glu Lys Met Cys Glu Thr Leu Asn Leu Lys Phe Met Ala Glu Val Gly 450 455 460 Thr Asn Arg Gly Leu Leu Pro Glu His Phe Leu Phe Leu Ala Gln Lys 465 470 475 480 Ile Phe Asn Asp Asn Ser Leu Ser Met Glu Ala Phe Gln His Arg Ser 485 490 495 Val Ser Trp Ser Gln Phe Asn Lys Glu Ile Leu Leu Gly Arg Gly Phe 500 505 510 Thr Phe Trp Gln Trp Phe Asp Gly Val Leu Asp Leu Thr Lys Arg Cys 515 520 525 Leu Arg Ser Tyr Trp Ser Asp Arg Leu Ile Ile Gly Phe Ile Ser Lys 530 535 540 Gln Tyr Val Thr Ser Leu Leu Leu Asn Glu Pro Asp Gly Thr Phe Leu 545 550 555 560 Leu Arg Phe Ser Asp Ser Glu Ile Gly Gly Ile Thr Ile Ala His Val 565 570 575 Ile Arg Gly Gln Asp Gly Ser Pro Gln Ile Glu Asn Ile Gln Pro Phe 580 585 590 Ser Ala Lys Asp Leu Ser Ile Arg Ser Leu Gly Asp Arg Ile Arg Asp 595 600 605 Leu Ala Gln Leu Lys Asn Leu Tyr Pro Lys Lys Pro Lys Asp Glu Ala 610 615 620 Phe Arg Ser His Tyr Lys Pro Glu Gln Met Gly Lys Asp Gly Arg Gly 625 630 635 640 Tyr Val Pro Ala Thr Ile Lys Met Thr Val Glu Arg Asp Gln Pro Leu 645 650 655 Pro Thr Pro Glu Leu Gln Met Pro Thr Met Val Pro Ser Tyr Asp Leu 660 665 670 Gly Met Ala Pro Asp Ser Ser Met Ser Met Gln Leu Gly Pro Asp Met 675 680 685 Val Pro Gln Val Tyr Pro Pro His Ser His Ser Ile Pro Pro Tyr Gln 690 695 700 Gly Leu Ser Pro Glu Glu Ser Val Asn Val Leu Ser Ala Phe Gln Glu 705 710 715 720 Pro His Leu Gln Met Pro Pro Ser Leu Gly Gln Met Ser Leu Pro Phe 725 730 735 Asp Gln Pro His Pro Gln Gly Leu Leu Pro Cys Gln Pro Gln Glu His 740 745 750 Ala Val Ser Ser Pro Asp Pro Leu Leu Cys Ser Asp Val Thr Met Val 755 760 765 Glu Asp Ser Cys Leu Ser Gln Pro Val Thr Ala Phe Pro Gln Gly Thr 770 775 780 Trp Ile Gly Glu Asp Ile Phe Pro Pro Leu Leu Pro Pro Thr Glu Gln 785 790 795 800 Asp Leu Thr Lys Leu Leu Leu Glu Gly Gln Gly Glu Ser Gly Gly Gly 805 810 815 Ser Leu Gly Ala Gln Pro Leu Leu Gln Pro Ser His Tyr Gly Gln Ser 820 825 830 Gly Ile Ser Met Ser His Met Asp Leu Arg Ala Asn Pro Ser Trp 835 840 845 < 210 > 80 < 211 > 847 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : STAT 6 ( S 407 D / V 547 A / T 548 A ) ; P 010 with no epitope tag < 400 > 80 Met Ser Leu Trp Gly Leu Val Ser Lys Met Pro Pro Glu Lys Val Gln 1 5 10 15 Arg Leu Tyr Val Asp Phe Pro Gln His Leu Arg His Leu Leu Gly Asp 20 25 30 Trp Leu Glu Ser Gln Pro Trp Glu Phe Leu Val Gly Ser Asp Ala Phe 35 40 45 Cys Cys Asn Leu Ala Ser Ala Leu Leu Ser Asp Thr Val Gln His Leu 50 55 60 Gln Ala Ser Val Gly Glu Gln Gly Glu Gly Ser Thr Ile Leu Gln His 65 70 75 80 Ile Ser Thr Leu Glu Ser Ile Tyr Gln Arg Asp Pro Leu Lys Leu Val 85 90 95 Ala Thr Phe Arg Gln Ile Leu Gln Gly Glu Lys Lys Ala Val Met Glu 100 105 110 Gln Phe Arg His Leu Pro Met Pro Phe His Trp Lys Gln Glu Glu Leu 115 120 125 Lys Phe Lys Thr Gly Leu Arg Arg Leu Gln His Arg Val Gly Glu Ile 130 135 140 His Leu Leu Arg Glu Ala Leu Gln Lys Gly Ala Glu Ala Gly Gln Val 145 150 155 160 Ser Leu His Ser Leu Ile Glu Thr Pro Ala Asn Gly Thr Gly Pro Ser 165 170 175 Glu Ala Leu Ala Met Leu Leu Gln Glu Thr Thr Gly Glu Leu Glu Ala 180 185 190 Ala Lys Ala Leu Val Leu Lys Arg Ile Gln Ile Trp Lys Arg Gln Gln 195 200 205 Gln Leu Ala Gly Asn Gly Ala Pro Phe Glu Glu Ser Leu Ala Pro Leu 210 215 220 Gln Glu Arg Cys Glu Ser Leu Val Asp Ile Tyr Ser Gln Leu Gln Gln 225 230 235 240 Glu Val Gly Ala Ala Gly Gly Glu Leu Glu Pro Lys Thr Arg Ala Ser 245 250 255 Leu Thr Gly Arg Leu Asp Glu Val Leu Arg Thr Leu Val Thr Ser Cys 260 265 270 Phe Leu Val Glu Lys Gln Pro Pro Gln Val Leu Lys Thr Gln Thr Lys 275 280 285 Phe Gln Ala Gly Val Arg Phe Leu Leu Gly Leu Arg Phe Leu Gly Ala 290 295 300 Pro Ala Lys Pro Pro Leu Val Arg Ala Asp Met Val Thr Glu Lys Gln 305 310 315 320 Ala Arg Glu Leu Ser Val Pro Gln Gly Pro Gly Ala Gly Ala Glu Ser 325 330 335 Thr Gly Glu Ile Ile Asn Asn Thr Val Pro Leu Glu Asn Ser Ile Pro 340 345 350 Gly Asn Cys Cys Ser Ala Leu Phe Lys Asn Leu Leu Leu Lys Lys Ile 355 360 365 Lys Arg Cys Glu Arg Lys Gly Thr Glu Ser Val Thr Glu Glu Lys Cys 370 375 380 Ala Val Leu Phe Ser Ala Ser Phe Thr Leu Gly Pro Gly Lys Leu Pro 385 390 395 400 Ile Gln Leu Gln Ala Leu Asp Leu Pro Leu Val Val Ile Val His Gly 405 410 415 Asn Gln Asp Asn Asn Ala Lys Ala Thr Ile Leu Trp Asp Asn Ala Phe 420 425 430 Ser Glu Met Asp Arg Val Pro Phe Val Val Ala Glu Arg Val Pro Trp 435 440 445 Glu Lys Met Cys Glu Thr Leu Asn Leu Lys Phe Met Ala Glu Val Gly 450 455 460 Thr Asn Arg Gly Leu Leu Pro Glu His Phe Leu Phe Leu Ala Gln Lys 465 470 475 480 Ile Phe Asn Asp Asn Ser Leu Ser Met Glu Ala Phe Gln His Arg Ser 485 490 495 Val Ser Trp Ser Gln Phe Asn Lys Glu Ile Leu Leu Gly Arg Gly Phe 500 505 510 Thr Phe Trp Gln Trp Phe Asp Gly Val Leu Asp Leu Thr Lys Arg Cys 515 520 525 Leu Arg Ser Tyr Trp Ser Asp Arg Leu Ile Ile Gly Phe Ile Ser Lys 530 535 540 Gln Tyr Ala Ala Ser Leu Leu Leu Asn Glu Pro Asp Gly Thr Phe Leu 545 550 555 560 Leu Arg Phe Ser Asp Ser Glu Ile Gly Gly Ile Thr Ile Ala His Val 565 570 575 Ile Arg Gly Gln Asp Gly Ser Pro Gln Ile Glu Asn Ile Gln Pro Phe 580 585 590 Ser Ala Lys Asp Leu Ser Ile Arg Ser Leu Gly Asp Arg Ile Arg Asp 595 600 605 Leu Ala Gln Leu Lys Asn Leu Tyr Pro Lys Lys Pro Lys Asp Glu Ala 610 615 620 Phe Arg Ser His Tyr Lys Pro Glu Gln Met Gly Lys Asp Gly Arg Gly 625 630 635 640 Tyr Val Pro Ala Thr Ile Lys Met Thr Val Glu Arg Asp Gln Pro Leu 645 650 655 Pro Thr Pro Glu Leu Gln Met Pro Thr Met Val Pro Ser Tyr Asp Leu 660 665 670 Gly Met Ala Pro Asp Ser Ser Met Ser Met Gln Leu Gly Pro Asp Met 675 680 685 Val Pro Gln Val Tyr Pro Pro His Ser His Ser Ile Pro Pro Tyr Gln 690 695 700 Gly Leu Ser Pro Glu Glu Ser Val Asn Val Leu Ser Ala Phe Gln Glu 705 710 715 720 Pro His Leu Gln Met Pro Pro Ser Leu Gly Gln Met Ser Leu Pro Phe 725 730 735 Asp Gln Pro His Pro Gln Gly Leu Leu Pro Cys Gln Pro Gln Glu His 740 745 750 Ala Val Ser Ser Pro Asp Pro Leu Leu Cys Ser Asp Val Thr Met Val 755 760 765 Glu Asp Ser Cys Leu Ser Gln Pro Val Thr Ala Phe Pro Gln Gly Thr 770 775 780 Trp Ile Gly Glu Asp Ile Phe Pro Pro Leu Leu Pro Pro Thr Glu Gln 785 790 795 800 Asp Leu Thr Lys Leu Leu Leu Glu Gly Gln Gly Glu Ser Gly Gly Gly 805 810 815 Ser Leu Gly Ala Gln Pro Leu Leu Gln Pro Ser His Tyr Gly Gln Ser 820 825 830 Gly Ile Ser Met Ser His Met Asp Leu Arg Ala Asn Pro Ser Trp 835 840 845 < 210 > 81 < 211 > 847 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : STAT 6 ( V 547 A / T 548 A / Y 641 F ) ; P 011 with no epitope tag < 400 > 81 Met Ser Leu Trp Gly Leu Val Ser Lys Met Pro Pro Glu Lys Val Gln 1 5 10 15 Arg Leu Tyr Val Asp Phe Pro Gln His Leu Arg His Leu Leu Gly Asp 20 25 30 Trp Leu Glu Ser Gln Pro Trp Glu Phe Leu Val Gly Ser Asp Ala Phe 35 40 45 Cys Cys Asn Leu Ala Ser Ala Leu Leu Ser Asp Thr Val Gln His Leu 50 55 60 Gln Ala Ser Val Gly Glu Gln Gly Glu Gly Ser Thr Ile Leu Gln His 65 70 75 80 Ile Ser Thr Leu Glu Ser Ile Tyr Gln Arg Asp Pro Leu Lys Leu Val 85 90 95 Ala Thr Phe Arg Gln Ile Leu Gln Gly Glu Lys Lys Ala Val Met Glu 100 105 110 Gln Phe Arg His Leu Pro Met Pro Phe His Trp Lys Gln Glu Glu Leu 115 120 125 Lys Phe Lys Thr Gly Leu Arg Arg Leu Gln His Arg Val Gly Glu Ile 130 135 140 His Leu Leu Arg Glu Ala Leu Gln Lys Gly Ala Glu Ala Gly Gln Val 145 150 155 160 Ser Leu His Ser Leu Ile Glu Thr Pro Ala Asn Gly Thr Gly Pro Ser 165 170 175 Glu Ala Leu Ala Met Leu Leu Gln Glu Thr Thr Gly Glu Leu Glu Ala 180 185 190 Ala Lys Ala Leu Val Leu Lys Arg Ile Gln Ile Trp Lys Arg Gln Gln 195 200 205 Gln Leu Ala Gly Asn Gly Ala Pro Phe Glu Glu Ser Leu Ala Pro Leu 210 215 220 Gln Glu Arg Cys Glu Ser Leu Val Asp Ile Tyr Ser Gln Leu Gln Gln 225 230 235 240 Glu Val Gly Ala Ala Gly Gly Glu Leu Glu Pro Lys Thr Arg Ala Ser 245 250 255 Leu Thr Gly Arg Leu Asp Glu Val Leu Arg Thr Leu Val Thr Ser Cys 260 265 270 Phe Leu Val Glu Lys Gln Pro Pro Gln Val Leu Lys Thr Gln Thr Lys 275 280 285 Phe Gln Ala Gly Val Arg Phe Leu Leu Gly Leu Arg Phe Leu Gly Ala 290 295 300 Pro Ala Lys Pro Pro Leu Val Arg Ala Asp Met Val Thr Glu Lys Gln 305 310 315 320 Ala Arg Glu Leu Ser Val Pro Gln Gly Pro Gly Ala Gly Ala Glu Ser 325 330 335 Thr Gly Glu Ile Ile Asn Asn Thr Val Pro Leu Glu Asn Ser Ile Pro 340 345 350 Gly Asn Cys Cys Ser Ala Leu Phe Lys Asn Leu Leu Leu Lys Lys Ile 355 360 365 Lys Arg Cys Glu Arg Lys Gly Thr Glu Ser Val Thr Glu Glu Lys Cys 370 375 380 Ala Val Leu Phe Ser Ala Ser Phe Thr Leu Gly Pro Gly Lys Leu Pro 385 390 395 400 Ile Gln Leu Gln Ala Leu Ser Leu Pro Leu Val Val Ile Val His Gly 405 410 415 Asn Gln Asp Asn Asn Ala Lys Ala Thr Ile Leu Trp Asp Asn Ala Phe 420 425 430 Ser Glu Met Asp Arg Val Pro Phe Val Val Ala Glu Arg Val Pro Trp 435 440 445 Glu Lys Met Cys Glu Thr Leu Asn Leu Lys Phe Met Ala Glu Val Gly 450 455 460 Thr Asn Arg Gly Leu Leu Pro Glu His Phe Leu Phe Leu Ala Gln Lys 465 470 475 480 Ile Phe Asn Asp Asn Ser Leu Ser Met Glu Ala Phe Gln His Arg Ser 485 490 495 Val Ser Trp Ser Gln Phe Asn Lys Glu Ile Leu Leu Gly Arg Gly Phe 500 505 510 Thr Phe Trp Gln Trp Phe Asp Gly Val Leu Asp Leu Thr Lys Arg Cys 515 520 525 Leu Arg Ser Tyr Trp Ser Asp Arg Leu Ile Ile Gly Phe Ile Ser Lys 530 535 540 Gln Tyr Ala Ala Ser Leu Leu Leu Asn Glu Pro Asp Gly Thr Phe Leu 545 550 555 560 Leu Arg Phe Ser Asp Ser Glu Ile Gly Gly Ile Thr Ile Ala His Val 565 570 575 Ile Arg Gly Gln Asp Gly Ser Pro Gln Ile Glu Asn Ile Gln Pro Phe 580 585 590 Ser Ala Lys Asp Leu Ser Ile Arg Ser Leu Gly Asp Arg Ile Arg Asp 595 600 605 Leu Ala Gln Leu Lys Asn Leu Tyr Pro Lys Lys Pro Lys Asp Glu Ala 610 615 620 Phe Arg Ser His Tyr Lys Pro Glu Gln Met Gly Lys Asp Gly Arg Gly 625 630 635 640 Phe Val Pro Ala Thr Ile Lys Met Thr Val Glu Arg Asp Gln Pro Leu 645 650 655 Pro Thr Pro Glu Leu Gln Met Pro Thr Met Val Pro Ser Tyr Asp Leu 660 665 670 Gly Met Ala Pro Asp Ser Ser Met Ser Met Gln Leu Gly Pro Asp Met 675 680 685 Val Pro Gln Val Tyr Pro Pro His Ser His Ser Ile Pro Pro Tyr Gln 690 695 700 Gly Leu Ser Pro Glu Glu Ser Val Asn Val Leu Ser Ala Phe Gln Glu 705 710 715 720 Pro His Leu Gln Met Pro Pro Ser Leu Gly Gln Met Ser Leu Pro Phe 725 730 735 Asp Gln Pro His Pro Gln Gly Leu Leu Pro Cys Gln Pro Gln Glu His 740 745 750 Ala Val Ser Ser Pro Asp Pro Leu Leu Cys Ser Asp Val Thr Met Val 755 760 765 Glu Asp Ser Cys Leu Ser Gln Pro Val Thr Ala Phe Pro Gln Gly Thr 770 775 780 Trp Ile Gly Glu Asp Ile Phe Pro Pro Leu Leu Pro Pro Thr Glu Gln 785 790 795 800 Asp Leu Thr Lys Leu Leu Leu Glu Gly Gln Gly Glu Ser Gly Gly Gly 805 810 815 Ser Leu Gly Ala Gln Pro Leu Leu Gln Pro Ser His Tyr Gly Gln Ser 820 825 830 Gly Ile Ser Met Ser His Met Asp Leu Arg Ala Asn Pro Ser Trp 835 840 845 < 210 > 82 < 211 > 479 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : hu - cFLIP - L ; P 1006 without epitope tag < 400 > 82 Ser Ala Glu Val Ile His Gln Val Glu Glu Ala Leu Asp Thr Asp Glu 1 5 10 15 Lys Glu Met Leu Leu Phe Leu Cys Arg Asp Val Ala Ile Asp Val Val 20 25 30 Pro Pro Asn Val Arg Asp Leu Leu Asp Ile Leu Arg Glu Arg Gly Lys 35 40 45 Leu Ser Val Gly Asp Leu Ala Glu Leu Leu Tyr Arg Val Arg Arg Phe 50 55 60 Asp Leu Leu Lys Arg Ile Leu Lys Met Asp Arg Lys Ala Val Glu Thr 65 70 75 80 His Leu Leu Arg Asn Pro His Leu Val Ser Asp Tyr Arg Val Leu Met 85 90 95 Ala Glu Ile Gly Glu Asp Leu Asp Lys Ser Asp Val Ser Ser Leu Ile 100 105 110 Phe Leu Met Lys Asp Tyr Met Gly Arg Gly Lys Ile Ser Lys Glu Lys 115 120 125 Ser Phe Leu Asp Leu Val Val Glu Leu Glu Lys Leu Asn Leu Val Ala 130 135 140 Pro Asp Gln Leu Asp Leu Leu Glu Lys Cys Leu Lys Asn Ile His Arg 145 150 155 160 Ile Asp Leu Lys Thr Lys Ile Gln Lys Tyr Lys Gln Ser Val Gln Gly 165 170 175 Ala Gly Thr Ser Tyr Arg Asn Val Leu Gln Ala Ala Ile Gln Lys Ser 180 185 190 Leu Lys Asp Pro Ser Asn Asn Phe Arg Leu His Asn Gly Arg Ser Lys 195 200 205 Glu Gln Arg Leu Lys Glu Gln Leu Gly Ala Gln Gln Glu Pro Val Lys 210 215 220 Lys Ser Ile Gln Glu Ser Glu Ala Phe Leu Pro Gln Ser Ile Pro Glu 225 230 235 240 Glu Arg Tyr Lys Met Lys Ser Lys Pro Leu Gly Ile Cys Leu Ile Ile 245 250 255 Asp Cys Ile Gly Asn Glu Thr Glu Leu Leu Arg Asp Thr Phe Thr Ser 260 265 270 Leu Gly Tyr Glu Val Gln Lys Phe Leu His Leu Ser Met His Gly Ile 275 280 285 Ser Gln Ile Leu Gly Gln Phe Ala Cys Met Pro Glu His Arg Asp Tyr 290 295 300 Asp Ser Phe Val Cys Val Leu Val Ser Arg Gly Gly Ser Gln Ser Val 305 310 315 320 Tyr Gly Val Asp Gln Thr His Ser Gly Leu Pro Leu His His Ile Arg 325 330 335 Arg Met Phe Met Gly Asp Ser Cys Pro Tyr Leu Ala Gly Lys Pro Lys 340 345 350 Met Phe Phe Ile Gln Asn Tyr Val Val Ser Glu Gly Gln Leu Glu Asp 355 360 365 Ser Ser Leu Leu Glu Val Asp Gly Pro Ala Met Lys Asn Val Glu Phe 370 375 380 Lys Ala Gln Lys Arg Gly Leu Cys Thr Val His Arg Glu Ala Asp Phe 385 390 395 400 Phe Trp Ser Leu Cys Thr Ala Asp Met Ser Leu Leu Glu Gln Ser His 405 410 415 Ser Ser Pro Ser Leu Tyr Leu Gln Cys Leu Ser Gln Lys Leu Arg Gln 420 425 430 Glu Arg Lys Arg Pro Leu Leu Asp Leu His Ile Glu Leu Asn Gly Tyr 435 440 445 Met Tyr Asp Trp Asn Ser Arg Val Ser Ala Lys Glu Lys Tyr Tyr Val 450 455 460 Trp Leu Gln His Thr Leu Arg Lys Lys Leu Ile Leu Ser Tyr Thr 465 470 475 < 210 > 83 < 211 > 226 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : hu - cFLIP - S ( 1 - 227 ) ; P 1007 without epitope tag < 400 > 83 Ser Ala Glu Val Ile His Gln Val Glu Glu Ala Leu Asp Thr Asp Glu 1 5 10 15 Lys Glu Met Leu Leu Phe Leu Cys Arg Asp Val Ala Ile Asp Val Val 20 25 30 Pro Pro Asn Val Arg Asp Leu Leu Asp Ile Leu Arg Glu Arg Gly Lys 35 40 45 Leu Ser Val Gly Asp Leu Ala Glu Leu Leu Tyr Arg Val Arg Arg Phe 50 55 60 Asp Leu Leu Lys Arg Ile Leu Lys Met Asp Arg Lys Ala Val Glu Thr 65 70 75 80 His Leu Leu Arg Asn Pro His Leu Val Ser Asp Tyr Arg Val Leu Met 85 90 95 Ala Glu Ile Gly Glu Asp Leu Asp Lys Ser Asp Val Ser Ser Leu Ile 100 105 110 Phe Leu Met Lys Asp Tyr Met Gly Arg Gly Lys Ile Ser Lys Glu Lys 115 120 125 Ser Phe Leu Asp Leu Val Val Glu Leu Glu Lys Leu Asn Leu Val Ala 130 135 140 Pro Asp Gln Leu Asp Leu Leu Glu Lys Cys Leu Lys Asn Ile His Arg 145 150 155 160 Ile Asp Leu Lys Thr Lys Ile Gln Lys Tyr Lys Gln Ser Val Gln Gly 165 170 175 Ala Gly Thr Ser Tyr Arg Asn Val Leu Gln Ala Ala Ile Gln Lys Ser 180 185 190 Leu Lys Asp Pro Ser Asn Asn Phe Arg Leu His Asn Gly Arg Ser Lys 195 200 205 Glu Gln Arg Leu Lys Glu Gln Leu Gly Ala Gln Gln Glu Pro Val Lys 210 215 220 Lys Ser 225 < 210 > 84 < 211 > 195 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : hu - cFLIP - p 22 ( 1 - 198 ) ; P 1008 without epitope tag < 400 > 84 Ser Ala Glu Val Ile His Gln Val Glu Glu Ala Leu Asp Thr Asp Glu 1 5 10 15 Lys Glu Met Leu Leu Phe Leu Cys Arg Asp Val Ala Ile Asp Val Val 20 25 30 Pro Pro Asn Val Arg Asp Leu Leu Asp Ile Leu Arg Glu Arg Gly Lys 35 40 45 Leu Ser Val Gly Asp Leu Ala Glu Leu Leu Tyr Arg Val Arg Arg Phe 50 55 60 Asp Leu Leu Lys Arg Ile Leu Lys Met Asp Arg Lys Ala Val Glu Thr 65 70 75 80 His Leu Leu Arg Asn Pro His Leu Val Ser Asp Tyr Arg Val Leu Met 85 90 95 Ala Glu Ile Gly Glu Asp Leu Asp Lys Ser Asp Val Ser Ser Leu Ile 100 105 110 Phe Leu Met Lys Asp Tyr Met Gly Arg Gly Lys Ile Ser Lys Glu Lys 115 120 125 Ser Phe Leu Asp Leu Val Val Glu Leu Glu Lys Leu Asn Leu Val Ala 130 135 140 Pro Asp Gln Leu Asp Leu Leu Glu Lys Cys Leu Lys Asn Ile His Arg 145 150 155 160 Ile Asp Leu Lys Thr Lys Ile Gln Lys Tyr Lys Gln Ser Val Gln Gly 165 170 175 Ala Gly Thr Ser Tyr Arg Asn Val Leu Gln Ala Ala Ile Gln Lys Ser 180 185 190 Leu Lys Asp 195 < 210 > 85 < 211 > 375 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : hu - cFLIP - p 43 ( 1 - 376 ) ; P 1009 without epitope tag < 400 > 85 Ser Ala Glu Val Ile His Gln Val Glu Glu Ala Leu Asp Thr Asp Glu 1 5 10 15 Lys Glu Met Leu Leu Phe Leu Cys Arg Asp Val Ala Ile Asp Val Val 20 25 30 Pro Pro Asn Val Arg Asp Leu Leu Asp Ile Leu Arg Glu Arg Gly Lys 35 40 45 Leu Ser Val Gly Asp Leu Ala Glu Leu Leu Tyr Arg Val Arg Arg Phe 50 55 60 Asp Leu Leu Lys Arg Ile Leu Lys Met Asp Arg Lys Ala Val Glu Thr 65 70 75 80 His Leu Leu Arg Asn Pro His Leu Val Ser Asp Tyr Arg Val Leu Met 85 90 95 Ala Glu Ile Gly Glu Asp Leu Asp Lys Ser Asp Val Ser Ser Leu Ile 100 105 110 Phe Leu Met Lys Asp Tyr Met Gly Arg Gly Lys Ile Ser Lys Glu Lys 115 120 125 Ser Phe Leu Asp Leu Val Val Glu Leu Glu Lys Leu Asn Leu Val Ala 130 135 140 Pro Asp Gln Leu Asp Leu Leu Glu Lys Cys Leu Lys Asn Ile His Arg 145 150 155 160 Ile Asp Leu Lys Thr Lys Ile Gln Lys Tyr Lys Gln Ser Val Gln Gly 165 170 175 Ala Gly Thr Ser Tyr Arg Asn Val Leu Gln Ala Ala Ile Gln Lys Ser 180 185 190 Leu Lys Asp Pro Ser Asn Asn Phe Arg Leu His Asn Gly Arg Ser Lys 195 200 205 Glu Gln Arg Leu Lys Glu Gln Leu Gly Ala Gln Gln Glu Pro Val Lys 210 215 220 Lys Ser Ile Gln Glu Ser Glu Ala Phe Leu Pro Gln Ser Ile Pro Glu 225 230 235 240 Glu Arg Tyr Lys Met Lys Ser Lys Pro Leu Gly Ile Cys Leu Ile Ile 245 250 255 Asp Cys Ile Gly Asn Glu Thr Glu Leu Leu Arg Asp Thr Phe Thr Ser 260 265 270 Leu Gly Tyr Glu Val Gln Lys Phe Leu His Leu Ser Met His Gly Ile 275 280 285 Ser Gln Ile Leu Gly Gln Phe Ala Cys Met Pro Glu His Arg Asp Tyr 290 295 300 Asp Ser Phe Val Cys Val Leu Val Ser Arg Gly Gly Ser Gln Ser Val 305 310 315 320 Tyr Gly Val Asp Gln Thr His Ser Gly Leu Pro Leu His His Ile Arg 325 330 335 Arg Met Phe Met Gly Asp Ser Cys Pro Tyr Leu Ala Gly Lys Pro Lys 340 345 350 Met Phe Phe Ile Gln Asn Tyr Val Val Ser Glu Gly Gln Leu Glu Asp 355 360 365 Ser Ser Leu Leu Glu Val Asp 370 375 < 210 > 86 < 211 > 104 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : hu - cFLIP - p 12 ( 377 - 480 ) ; P 1010 without epitope tag < 400 > 86 Gly Pro Ala Met Lys Asn Val Glu Phe Lys Ala Gln Lys Arg Gly Leu 1 5 10 15 Cys Thr Val His Arg Glu Ala Asp Phe Phe Trp Ser Leu Cys Thr Ala 20 25 30 Asp Met Ser Leu Leu Glu Gln Ser His Ser Ser Pro Ser Leu Tyr Leu 35 40 45 Gln Cys Leu Ser Gln Lys Leu Arg Gln Glu Arg Lys Arg Pro Leu Leu 50 55 60 Asp Leu His Ile Glu Leu Asn Gly Tyr Met Tyr Asp Trp Asn Ser Arg 65 70 75 80 Val Ser Ala Lys Glu Lys Tyr Tyr Val Trp Leu Gln His Thr Leu Arg 85 90 95 Lys Lys Leu Ile Leu Ser Tyr Thr 100 < 210 > 87 < 211 > 756 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : huIKK 2 ca ( S 177 E / S 181 E ) ; P 4005 without epitope tag < 400 > 87 Met Ser Trp Ser Pro Ser Leu Thr Thr Gln Thr Cys Gly Ala Trp Glu 1 5 10 15 Met Lys Glu Arg Leu Gly Thr Gly Gly Phe Gly Asn Val Ile Arg Trp 20 25 30 His Asn Gln Glu Thr Gly Glu Gln Ile Ala Ile Lys Gln Cys Arg Gln 35 40 45 Glu Leu Ser Pro Arg Asn Arg Glu Arg Trp Cys Leu Glu Ile Gln Ile 50 55 60 Met Arg Arg Leu Thr His Pro Asn Val Val Ala Ala Arg Asp Val Pro 65 70 75 80 Glu Gly Met Gln Asn Leu Ala Pro Asn Asp Leu Pro Leu Leu Ala Met 85 90 95 Glu Tyr Cys Gln Gly Gly Asp Leu Arg Lys Tyr Leu Asn Gln Phe Glu 100 105 110 Asn Cys Cys Gly Leu Arg Glu Gly Ala Ile Leu Thr Leu Leu Ser Asp 115 120 125 Ile Ala Ser Ala Leu Arg Tyr Leu His Glu Asn Arg Ile Ile His Arg 130 135 140 Asp Leu Lys Pro Glu Asn Ile Val Leu Gln Gln Gly Glu Gln Arg Leu 145 150 155 160 Ile His Lys Ile Ile Asp Leu Gly Tyr Ala Lys Glu Leu Asp Gln Gly 165 170 175 Glu Leu Cys Thr Glu Phe Val Gly Thr Leu Gln Tyr Leu Ala Pro Glu 180 185 190 Leu Leu Glu Gln Gln Lys Tyr Thr Val Thr Val Asp Tyr Trp Ser Phe 195 200 205 Gly Thr Leu Ala Phe Glu Cys Ile Thr Gly Phe Arg Pro Phe Leu Pro 210 215 220 Asn Trp Gln Pro Val Gln Trp His Ser Lys Val Arg Gln Lys Ser Glu 225 230 235 240 Val Asp Ile Val Val Ser Glu Asp Leu Asn Gly Thr Val Lys Phe Ser 245 250 255 Ser Ser Leu Pro Tyr Pro Asn Asn Leu Asn Ser Val Leu Ala Glu Arg 260 265 270 Leu Glu Lys Trp Leu Gln Leu Met Leu Met Trp His Pro Arg Gln Arg 275 280 285 Gly Thr Asp Pro Thr Tyr Gly Pro Asn Gly Cys Phe Lys Ala Leu Asp 290 295 300 Asp Ile Leu Asn Leu Lys Leu Val His Ile Leu Asn Met Val Thr Gly 305 310 315 320 Thr Ile His Thr Tyr Pro Val Thr Glu Asp Glu Ser Leu Gln Ser Leu 325 330 335 Lys Ala Arg Ile Gln Gln Asp Thr Gly Ile Pro Glu Glu Asp Gln Glu 340 345 350 Leu Leu Gln Glu Ala Gly Leu Ala Leu Ile Pro Asp Lys Pro Ala Thr 355 360 365 Gln Cys Ile Ser Asp Gly Lys Leu Asn Glu Gly His Thr Leu Asp Met 370 375 380 Asp Leu Val Phe Leu Phe Asp Asn Ser Lys Ile Thr Tyr Glu Thr Gln 385 390 395 400 Ile Ser Pro Arg Pro Gln Pro Glu Ser Val Ser Cys Ile Leu Gln Glu 405 410 415 Pro Lys Arg Asn Leu Ala Phe Phe Gln Leu Arg Lys Val Trp Gly Gln 420 425 430 Val Trp His Ser Ile Gln Thr Leu Lys Glu Asp Cys Asn Arg Leu Gln 435 440 445 Gln Gly Gln Arg Ala Ala Met Met Asn Leu Leu Arg Asn Asn Ser Cys 450 455 460 Leu Ser Lys Met Lys Asn Ser Met Ala Ser Met Ser Gln Gln Leu Lys 465 470 475 480 Ala Lys Leu Asp Phe Phe Lys Thr Ser Ile Gln Ile Asp Leu Glu Lys 485 490 495 Tyr Ser Glu Gln Thr Glu Phe Gly Ile Thr Ser Asp Lys Leu Leu Leu 500 505 510 Ala Trp Arg Glu Met Glu Gln Ala Val Glu Leu Cys Gly Arg Glu Asn 515 520 525 Glu Val Lys Leu Leu Val Glu Arg Met Met Ala Leu Gln Thr Asp Ile 530 535 540 Val Asp Leu Gln Arg Ser Pro Met Gly Arg Lys Gln Gly Gly Thr Leu 545 550 555 560 Asp Asp Leu Glu Glu Gln Ala Arg Glu Leu Tyr Arg Arg Leu Arg Glu 565 570 575 Lys Pro Arg Asp Gln Arg Thr Glu Gly Asp Ser Gln Glu Met Val Arg 580 585 590 Leu Leu Leu Gln Ala Ile Gln Ser Phe Glu Lys Lys Val Arg Val Ile 595 600 605 Tyr Thr Gln Leu Ser Lys Thr Val Val Cys Lys Gln Lys Ala Leu Glu 610 615 620 Leu Leu Pro Lys Val Glu Glu Val Val Ser Leu Met Asn Glu Asp Glu 625 630 635 640 Lys Thr Val Val Arg Leu Gln Glu Lys Arg Gln Lys Glu Leu Trp Asn 645 650 655 Leu Leu Lys Ile Ala Cys Ser Lys Val Arg Gly Pro Val Ser Gly Ser 660 665 670 Pro Asp Ser Met Asn Ala Ser Arg Leu Ser Gln Pro Gly Gln Leu Met 675 680 685 Ser Gln Pro Ser Thr Ala Ser Asn Ser Leu Pro Glu Pro Ala Lys Lys 690 695 700 Ser Glu Glu Leu Val Ala Glu Ala His Asn Leu Cys Thr Leu Leu Glu 705 710 715 720 Asn Ala Ile Gln Asp Thr Val Arg Glu Gln Asp Gln Ser Phe Thr Ala 725 730 735 Leu Asp Trp Ser Trp Leu Gln Thr Glu Glu Glu Glu His Ser Cys Leu 740 745 750 Glu Gln Ala Ser 755 < 210 > 88 < 211 > 756 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : huIKK 2 null ( S 177 A / S 181 A ) ; P 4006 without epitope tag < 400 > 88 Met Ser Trp Ser Pro Ser Leu Thr Thr Gln Thr Cys Gly Ala Trp Glu 1 5 10 15 Met Lys Glu Arg Leu Gly Thr Gly Gly Phe Gly Asn Val Ile Arg Trp 20 25 30 His Asn Gln Glu Thr Gly Glu Gln Ile Ala Ile Lys Gln Cys Arg Gln 35 40 45 Glu Leu Ser Pro Arg Asn Arg Glu Arg Trp Cys Leu Glu Ile Gln Ile 50 55 60 Met Arg Arg Leu Thr His Pro Asn Val Val Ala Ala Arg Asp Val Pro 65 70 75 80 Glu Gly Met Gln Asn Leu Ala Pro Asn Asp Leu Pro Leu Leu Ala Met 85 90 95 Glu Tyr Cys Gln Gly Gly Asp Leu Arg Lys Tyr Leu Asn Gln Phe Glu 100 105 110 Asn Cys Cys Gly Leu Arg Glu Gly Ala Ile Leu Thr Leu Leu Ser Asp 115 120 125 Ile Ala Ser Ala Leu Arg Tyr Leu His Glu Asn Arg Ile Ile His Arg 130 135 140 Asp Leu Lys Pro Glu Asn Ile Val Leu Gln Gln Gly Glu Gln Arg Leu 145 150 155 160 Ile His Lys Ile Ile Asp Leu Gly Tyr Ala Lys Glu Leu Asp Gln Gly 165 170 175 Ala Leu Cys Thr Ala Phe Val Gly Thr Leu Gln Tyr Leu Ala Pro Glu 180 185 190 Leu Leu Glu Gln Gln Lys Tyr Thr Val Thr Val Asp Tyr Trp Ser Phe 195 200 205 Gly Thr Leu Ala Phe Glu Cys Ile Thr Gly Phe Arg Pro Phe Leu Pro 210 215 220 Asn Trp Gln Pro Val Gln Trp His Ser Lys Val Arg Gln Lys Ser Glu 225 230 235 240 Val Asp Ile Val Val Ser Glu Asp Leu Asn Gly Thr Val Lys Phe Ser 245 250 255 Ser Ser Leu Pro Tyr Pro Asn Asn Leu Asn Ser Val Leu Ala Glu Arg 260 265 270 Leu Glu Lys Trp Leu Gln Leu Met Leu Met Trp His Pro Arg Gln Arg 275 280 285 Gly Thr Asp Pro Thr Tyr Gly Pro Asn Gly Cys Phe Lys Ala Leu Asp 290 295 300 Asp Ile Leu Asn Leu Lys Leu Val His Ile Leu Asn Met Val Thr Gly 305 310 315 320 Thr Ile His Thr Tyr Pro Val Thr Glu Asp Glu Ser Leu Gln Ser Leu 325 330 335 Lys Ala Arg Ile Gln Gln Asp Thr Gly Ile Pro Glu Glu Asp Gln Glu 340 345 350 Leu Leu Gln Glu Ala Gly Leu Ala Leu Ile Pro Asp Lys Pro Ala Thr 355 360 365 Gln Cys Ile Ser Asp Gly Lys Leu Asn Glu Gly His Thr Leu Asp Met 370 375 380 Asp Leu Val Phe Leu Phe Asp Asn Ser Lys Ile Thr Tyr Glu Thr Gln 385 390 395 400 Ile Ser Pro Arg Pro Gln Pro Glu Ser Val Ser Cys Ile Leu Gln Glu 405 410 415 Pro Lys Arg Asn Leu Ala Phe Phe Gln Leu Arg Lys Val Trp Gly Gln 420 425 430 Val Trp His Ser Ile Gln Thr Leu Lys Glu Asp Cys Asn Arg Leu Gln 435 440 445 Gln Gly Gln Arg Ala Ala Met Met Asn Leu Leu Arg Asn Asn Ser Cys 450 455 460 Leu Ser Lys Met Lys Asn Ser Met Ala Ser Met Ser Gln Gln Leu Lys 465 470 475 480 Ala Lys Leu Asp Phe Phe Lys Thr Ser Ile Gln Ile Asp Leu Glu Lys 485 490 495 Tyr Ser Glu Gln Thr Glu Phe Gly Ile Thr Ser Asp Lys Leu Leu Leu 500 505 510 Ala Trp Arg Glu Met Glu Gln Ala Val Glu Leu Cys Gly Arg Glu Asn 515 520 525 Glu Val Lys Leu Leu Val Glu Arg Met Met Ala Leu Gln Thr Asp Ile 530 535 540 Val Asp Leu Gln Arg Ser Pro Met Gly Arg Lys Gln Gly Gly Thr Leu 545 550 555 560 Asp Asp Leu Glu Glu Gln Ala Arg Glu Leu Tyr Arg Arg Leu Arg Glu 565 570 575 Lys Pro Arg Asp Gln Arg Thr Glu Gly Asp Ser Gln Glu Met Val Arg 580 585 590 Leu Leu Leu Gln Ala Ile Gln Ser Phe Glu Lys Lys Val Arg Val Ile 595 600 605 Tyr Thr Gln Leu Ser Lys Thr Val Val Cys Lys Gln Lys Ala Leu Glu 610 615 620 Leu Leu Pro Lys Val Glu Glu Val Val Ser Leu Met Asn Glu Asp Glu 625 630 635 640 Lys Thr Val Val Arg Leu Gln Glu Lys Arg Gln Lys Glu Leu Trp Asn 645 650 655 Leu Leu Lys Ile Ala Cys Ser Lys Val Arg Gly Pro Val Ser Gly Ser 660 665 670 Pro Asp Ser Met Asn Ala Ser Arg Leu Ser Gln Pro Gly Gln Leu Met 675 680 685 Ser Gln Pro Ser Thr Ala Ser Asn Ser Leu Pro Glu Pro Ala Lys Lys 690 695 700 Ser Glu Glu Leu Val Ala Glu Ala His Asn Leu Cys Thr Leu Leu Glu 705 710 715 720 Asn Ala Ile Gln Asp Thr Val Arg Glu Gln Asp Gln Ser Phe Thr Ala 725 730 735 Leu Asp Trp Ser Trp Leu Gln Thr Glu Glu Glu Glu His Ser Cys Leu 740 745 750 Glu Gln Ala Ser 755 < 210 > 89 < 211 > 757 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : muIKK 2 ca ( S 177 E / S 181 E ) ; P 4002 without epitope tag < 400 > 89 Met Ser Trp Ser Pro Ser Leu Pro Thr Gln Thr Cys Gly Ala Trp Glu 1 5 10 15 Met Lys Glu Arg Leu Gly Thr Gly Gly Phe Gly Asn Val Ile Arg Trp 20 25 30 His Asn Gln Ala Thr Gly Glu Gln Ile Ala Ile Lys Gln Cys Arg Gln 35 40 45 Glu Leu Ser Pro Lys Asn Arg Asn Arg Trp Cys Leu Glu Ile Gln Ile 50 55 60 Met Arg Arg Leu Asn His Pro Asn Val Val Ala Ala Arg Asp Val Pro 65 70 75 80 Glu Gly Met Gln Asn Leu Ala Pro Asn Asp Leu Pro Leu Leu Ala Met 85 90 95 Glu Tyr Cys Gln Gly Gly Asp Leu Arg Arg Tyr Leu Asn Gln Phe Glu 100 105 110 Asn Cys Cys Gly Leu Arg Glu Gly Ala Val Leu Thr Leu Leu Ser Asp 115 120 125 Ile Ala Ser Ala Leu Arg Tyr Leu His Glu Asn Arg Ile Ile His Arg 130 135 140 Asp Leu Lys Pro Glu Asn Ile Val Leu Gln Gln Gly Glu Lys Arg Leu 145 150 155 160 Ile His Lys Ile Ile Asp Leu Gly Tyr Ala Lys Glu Leu Asp Gln Gly 165 170 175 Glu Leu Cys Thr Glu Phe Val Gly Thr Leu Gln Tyr Leu Ala Pro Glu 180 185 190 Leu Leu Glu Gln Gln Lys Tyr Thr Val Thr Val Asp Tyr Trp Ser Phe 195 200 205 Gly Thr Leu Ala Phe Glu Cys Ile Thr Gly Phe Arg Pro Phe Leu Pro 210 215 220 Asn Trp Gln Pro Val Gln Trp His Ser Lys Val Arg Gln Lys Ser Glu 225 230 235 240 Val Asp Ile Val Val Ser Glu Asp Leu Asn Gly Ala Val Lys Phe Ser 245 250 255 Ser Ser Leu Pro Phe Pro Asn Asn Leu Asn Ser Val Leu Ala Glu Arg 260 265 270 Leu Glu Lys Trp Leu Gln Leu Met Leu Met Trp His Pro Arg Gln Arg 275 280 285 Gly Thr Asp Pro Gln Tyr Gly Pro Asn Gly Cys Phe Arg Ala Leu Asp 290 295 300 Asp Ile Leu Asn Leu Lys Leu Val His Val Leu Asn Met Val Thr Gly 305 310 315 320 Thr Val His Thr Tyr Pro Val Thr Glu Asp Glu Ser Leu Gln Ser Leu 325 330 335 Lys Thr Arg Ile Gln Glu Asn Thr Gly Ile Leu Glu Thr Asp Gln Glu 340 345 350 Leu Leu Gln Lys Ala Gly Leu Val Leu Leu Pro Asp Lys Pro Ala Thr 355 360 365 Gln Cys Ile Ser Asp Ser Lys Thr Asn Glu Gly Leu Thr Leu Asp Met 370 375 380 Asp Leu Val Phe Leu Leu Asp Asn Ser Lys Ile Asn Tyr Glu Thr Gln 385 390 395 400 Ile Thr Pro Arg Pro Pro Pro Glu Ser Val Ser Cys Ile Leu Gln Glu 405 410 415 Pro Lys Arg Asn Leu Ser Phe Phe Gln Leu Arg Lys Val Trp Gly Gln 420 425 430 Val Trp His Ser Ile Gln Thr Leu Lys Glu Asp Cys Asn Arg Leu Gln 435 440 445 Gln Gly Gln Arg Ala Ala Met Met Ser Leu Leu Arg Asn Asn Ser Cys 450 455 460 Leu Ser Lys Met Lys Asn Ala Met Ala Ser Thr Ala Gln Gln Leu Lys 465 470 475 480 Ala Lys Leu Asp Phe Phe Lys Thr Ser Ile Gln Ile Asp Leu Glu Lys 485 490 495 Tyr Lys Glu Gln Thr Glu Phe Gly Ile Thr Ser Asp Lys Leu Leu Leu 500 505 510 Ala Trp Arg Glu Met Glu Gln Ala Val Glu Gln Cys Gly Arg Glu Asn 515 520 525 Asp Val Lys His Leu Val Glu Arg Met Met Ala Leu Gln Thr Asp Ile 530 535 540 Val Asp Leu Gln Arg Ser Pro Met Gly Arg Lys Gln Gly Gly Thr Leu 545 550 555 560 Asp Asp Leu Glu Glu Gln Ala Arg Glu Leu Tyr Arg Lys Leu Arg Glu 565 570 575 Lys Pro Arg Asp Gln Arg Thr Glu Gly Asp Ser Gln Glu Met Val Arg 580 585 590 Leu Leu Leu Gln Ala Ile Gln Ser Phe Glu Lys Lys Val Arg Val Ile 595 600 605 Tyr Thr Gln Leu Ser Lys Thr Val Val Cys Lys Gln Lys Ala Leu Glu 610 615 620 Leu Leu Pro Lys Val Glu Glu Val Val Ser Leu Met Asn Glu Asp Glu 625 630 635 640 Arg Thr Val Val Arg Leu Gln Glu Lys Arg Gln Lys Glu Leu Trp Asn 645 650 655 Leu Leu Lys Ile Ala Cys Ser Lys Val Arg Gly Pro Val Ser Gly Ser 660 665 670 Pro Asp Ser Met Asn Val Ser Arg Leu Ser His Pro Gly Gln Leu Met 675 680 685 Ser Gln Pro Ser Ser Ala Cys Asp Ser Leu Pro Glu Ser Asp Lys Lys 690 695 700 Ser Glu Glu Leu Val Ala Glu Ala His Ala Leu Cys Ser Arg Leu Glu 705 710 715 720 Ser Ala Leu Gln Asp Thr Val Lys Glu Gln Asp Arg Ser Phe Thr Thr 725 730 735 Leu Asp Trp Ser Trp Leu Gln Met Glu Asp Glu Glu Arg Cys Ser Leu 740 745 750 Glu Gln Ala Cys Asp 755 < 210 > 90 < 211 > 757 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : muIKK 2 null ( S 177 A / S 181 A ) ; P 4003 without epitope tag < 400 > 90 Met Ser Trp Ser Pro Ser Leu Pro Thr Gln Thr Cys Gly Ala Trp Glu 1 5 10 15 Met Lys Glu Arg Leu Gly Thr Gly Gly Phe Gly Asn Val Ile Arg Trp 20 25 30 His Asn Gln Ala Thr Gly Glu Gln Ile Ala Ile Lys Gln Cys Arg Gln 35 40 45 Glu Leu Ser Pro Lys Asn Arg Asn Arg Trp Cys Leu Glu Ile Gln Ile 50 55 60 Met Arg Arg Leu Asn His Pro Asn Val Val Ala Ala Arg Asp Val Pro 65 70 75 80 Glu Gly Met Gln Asn Leu Ala Pro Asn Asp Leu Pro Leu Leu Ala Met 85 90 95 Glu Tyr Cys Gln Gly Gly Asp Leu Arg Arg Tyr Leu Asn Gln Phe Glu 100 105 110 Asn Cys Cys Gly Leu Arg Glu Gly Ala Val Leu Thr Leu Leu Ser Asp 115 120 125 Ile Ala Ser Ala Leu Arg Tyr Leu His Glu Asn Arg Ile Ile His Arg 130 135 140 Asp Leu Lys Pro Glu Asn Ile Val Leu Gln Gln Gly Glu Lys Arg Leu 145 150 155 160 Ile His Lys Ile Ile Asp Leu Gly Tyr Ala Lys Glu Leu Asp Gln Gly 165 170 175 Ala Leu Cys Thr Ala Phe Val Gly Thr Leu Gln Tyr Leu Ala Pro Glu 180 185 190 Leu Leu Glu Gln Gln Lys Tyr Thr Val Thr Val Asp Tyr Trp Ser Phe 195 200 205 Gly Thr Leu Ala Phe Glu Cys Ile Thr Gly Phe Arg Pro Phe Leu Pro 210 215 220 Asn Trp Gln Pro Val Gln Trp His Ser Lys Val Arg Gln Lys Ser Glu 225 230 235 240 Val Asp Ile Val Val Ser Glu Asp Leu Asn Gly Ala Val Lys Phe Ser 245 250 255 Ser Ser Leu Pro Phe Pro Asn Asn Leu Asn Ser Val Leu Ala Glu Arg 260 265 270 Leu Glu Lys Trp Leu Gln Leu Met Leu Met Trp His Pro Arg Gln Arg 275 280 285 Gly Thr Asp Pro Gln Tyr Gly Pro Asn Gly Cys Phe Arg Ala Leu Asp 290 295 300 Asp Ile Leu Asn Leu Lys Leu Val His Val Leu Asn Met Val Thr Gly 305 310 315 320 Thr Val His Thr Tyr Pro Val Thr Glu Asp Glu Ser Leu Gln Ser Leu 325 330 335 Lys Thr Arg Ile Gln Glu Asn Thr Gly Ile Leu Glu Thr Asp Gln Glu 340 345 350 Leu Leu Gln Lys Ala Gly Leu Val Leu Leu Pro Asp Lys Pro Ala Thr 355 360 365 Gln Cys Ile Ser Asp Ser Lys Thr Asn Glu Gly Leu Thr Leu Asp Met 370 375 380 Asp Leu Val Phe Leu Leu Asp Asn Ser Lys Ile Asn Tyr Glu Thr Gln 385 390 395 400 Ile Thr Pro Arg Pro Pro Pro Glu Ser Val Ser Cys Ile Leu Gln Glu 405 410 415 Pro Lys Arg Asn Leu Ser Phe Phe Gln Leu Arg Lys Val Trp Gly Gln 420 425 430 Val Trp His Ser Ile Gln Thr Leu Lys Glu Asp Cys Asn Arg Leu Gln 435 440 445 Gln Gly Gln Arg Ala Ala Met Met Ser Leu Leu Arg Asn Asn Ser Cys 450 455 460 Leu Ser Lys Met Lys Asn Ala Met Ala Ser Thr Ala Gln Gln Leu Lys 465 470 475 480 Ala Lys Leu Asp Phe Phe Lys Thr Ser Ile Gln Ile Asp Leu Glu Lys 485 490 495 Tyr Lys Glu Gln Thr Glu Phe Gly Ile Thr Ser Asp Lys Leu Leu Leu 500 505 510 Ala Trp Arg Glu Met Glu Gln Ala Val Glu Gln Cys Gly Arg Glu Asn 515 520 525 Asp Val Lys His Leu Val Glu Arg Met Met Ala Leu Gln Thr Asp Ile 530 535 540 Val Asp Leu Gln Arg Ser Pro Met Gly Arg Lys Gln Gly Gly Thr Leu 545 550 555 560 Asp Asp Leu Glu Glu Gln Ala Arg Glu Leu Tyr Arg Lys Leu Arg Glu 565 570 575 Lys Pro Arg Asp Gln Arg Thr Glu Gly Asp Ser Gln Glu Met Val Arg 580 585 590 Leu Leu Leu Gln Ala Ile Gln Ser Phe Glu Lys Lys Val Arg Val Ile 595 600 605 Tyr Thr Gln Leu Ser Lys Thr Val Val Cys Lys Gln Lys Ala Leu Glu 610 615 620 Leu Leu Pro Lys Val Glu Glu Val Val Ser Leu Met Asn Glu Asp Glu 625 630 635 640 Arg Thr Val Val Arg Leu Gln Glu Lys Arg Gln Lys Glu Leu Trp Asn 645 650 655 Leu Leu Lys Ile Ala Cys Ser Lys Val Arg Gly Pro Val Ser Gly Ser 660 665 670 Pro Asp Ser Met Asn Val Ser Arg Leu Ser His Pro Gly Gln Leu Met 675 680 685 Ser Gln Pro Ser Ser Ala Cys Asp Ser Leu Pro Glu Ser Asp Lys Lys 690 695 700 Ser Glu Glu Leu Val Ala Glu Ala His Ala Leu Cys Ser Arg Leu Glu 705 710 715 720 Ser Ala Leu Gln Asp Thr Val Lys Glu Gln Asp Arg Ser Phe Thr Thr 725 730 735 Leu Asp Trp Ser Trp Leu Gln Met Glu Asp Glu Glu Arg Cys Ser Leu 740 745 750 Glu Gln Ala Cys Asp 755 < 210 > 91 < 211 > 744 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 744 ) < 223 > Human constitutively active IKK alpha ( PEST mutation ) P . 4013 without epitope tag < 400 > 91 Met Glu Arg Pro Pro Gly Leu Arg Pro Gly Ala Gly Gly Pro Trp Glu 1 5 10 15 Met Arg Glu Arg Leu Gly Thr Gly Gly Phe Gly Asn Val Cys Leu Tyr 20 25 30 Gln His Arg Glu Leu Asp Leu Lys Ile Ala Ile Lys Ser Cys Arg Leu 35 40 45 Glu Leu Ser Thr Lys Asn Arg Glu Arg Trp Cys His Glu Ile Gln Ile 50 55 60 Met Lys Lys Leu Asn His Ala Asn Val Val Lys Ala Cys Asp Val Pro 65 70 75 80 Glu Glu Leu Asn Ile Leu Ile His Asp Val Pro Leu Leu Ala Met Glu 85 90 95 Tyr Cys Ser Gly Gly Asp Leu Arg Lys Leu Leu Asn Lys Pro Glu Asn 100 105 110 Cys Cys Gly Leu Lys Glu Ser Gln Ile Leu Ser Leu Leu Ser Asp Ile 115 120 125 Gly Ser Gly Ile Arg Tyr Leu His Glu Asn Lys Ile Ile His Arg Asp 130 135 140 Leu Lys Pro Glu Asn Ile Val Leu Gln Asp Val Gly Gly Lys Ile Ile 145 150 155 160 His Lys Ile Ile Asp Leu Gly Tyr Ala Lys Asp Val Asp Gln Gly Glu 165 170 175 Leu Cys Thr Glu Phe Val Gly Thr Leu Gln Tyr Leu Ala Pro Glu Leu 180 185 190 Phe Glu Asn Lys Pro Tyr Thr Ala Thr Val Asp Tyr Trp Ser Phe Gly 195 200 205 Thr Met Val Phe Glu Cys Ile Ala Gly Tyr Arg Pro Phe Leu His His 210 215 220 Leu Gln Pro Phe Thr Trp His Glu Lys Ile Lys Lys Lys Asp Pro Lys 225 230 235 240 Cys Ile Phe Ala Cys Glu Glu Met Ser Gly Glu Val Arg Phe Ser Ser 245 250 255 His Leu Pro Gln Pro Asn Ser Leu Cys Ser Leu Val Val Glu Pro Met 260 265 270 Glu Asn Trp Leu Gln Leu Met Leu Asn Trp Asp Pro Gln Gln Arg Gly 275 280 285 Gly Pro Val Asp Leu Thr Leu Lys Gln Pro Arg Cys Phe Val Leu Met 290 295 300 Asp His Ile Leu Asn Leu Lys Ile Val His Ile Leu Asn Met Thr Ser 305 310 315 320 Ala Lys Ile Ile Ser Phe Leu Leu Pro Pro Asp Glu Ser Leu His Ser 325 330 335 Leu Gln Ser Arg Ile Glu Arg Glu Thr Gly Ile Asn Thr Gly Ser Gln 340 345 350 Glu Leu Leu Ser Glu Thr Gly Ile Ser Leu Asp Pro Arg Lys Pro Ala 355 360 365 Ser Gln Cys Val Leu Asp Gly Val Arg Gly Cys Asp Ser Tyr Met Val 370 375 380 Tyr Leu Phe Asp Lys Ser Lys Thr Val Tyr Glu Gly Pro Phe Ala Ser 385 390 395 400 Arg Ser Leu Ser Asp Cys Val Asn Tyr Ile Val Gln Asp Ser Lys Ile 405 410 415 Gln Leu Pro Ile Ile Gln Leu Arg Lys Val Trp Ala Glu Ala Val His 420 425 430 Tyr Val Ser Gly Leu Lys Glu Asp Tyr Ser Arg Leu Phe Gln Gly Gln 435 440 445 Arg Ala Ala Met Leu Ser Leu Leu Arg Tyr Asn Ala Asn Leu Thr Lys 450 455 460 Met Lys Asn Thr Leu Ile Ser Ala Ser Gln Gln Leu Lys Ala Lys Leu 465 470 475 480 Glu Phe Phe His Lys Ser Ile Gln Leu Asp Leu Glu Arg Tyr Ser Glu 485 490 495 Gln Met Thr Tyr Gly Ile Ser Ser Glu Lys Met Leu Lys Ala Trp Lys 500 505 510 Glu Met Glu Glu Lys Ala Ile His Tyr Ala Glu Val Gly Val Ile Gly 515 520 525 Tyr Leu Glu Asp Gln Ile Met Ser Leu His Ala Glu Ile Met Glu Leu 530 535 540 Gln Lys Ser Pro Tyr Arg Arg Gln Gly Asp Leu Met Glu Ser Leu Glu 545 550 555 560 Gln Arg Ala Ile Asp Leu Tyr Lys Gln Leu Lys His Arg Pro Ser Asp 565 570 575 His Ser Tyr Ser Asp Ser Thr Glu Met Val Lys Ile Ile Val His Thr 580 585 590 Val Gln Ser Gln Asp Arg Val Leu Lys Glu Leu Phe Gly His Leu Ser 595 600 605 Lys Leu Leu Gly Cys Lys Gln Lys Ile Ile Asp Leu Leu Pro Lys Val 610 615 620 Glu Val Ala Leu Ser Asn Ile Lys Glu Ala Asp Asn Thr Val Met Phe 625 630 635 640 Met Gln Gly Lys Arg Gln Lys Glu Ile Trp His Leu Leu Lys Ile Ala 645 650 655 Cys Thr Gln Ala Ala Ala Arg Ala Leu Val Gly Ala Ala Leu Glu Gly 660 665 670 Ala Val Ala Pro Gln Ala Ala Ala Trp Leu Pro Pro Ala Ala Ala Glu 675 680 685 His Asp His Ala Leu Ala Cys Val Val Ala Pro Gln Asp Gly Glu Ala 690 695 700 Ala Ala Gln Met Ile Glu Glu Asn Leu Asn Cys Leu Gly His Leu Ala 705 710 715 720 Ala Ile Ile His Glu Ala Asn Glu Glu Gln Gly Asn Ser Met Met Asn 725 730 735 Leu Asp Trp Ser Trp Leu Thr Glu 740 < 210 > 92 < 211 > 744 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 744 ) < 223 > Human constitutively active IKK alpha ( PEST mutation ) P . 4014 without epitope tag < 400 > 92 Met Glu Arg Pro Pro Gly Leu Arg Pro Gly Ala Gly Gly Pro Trp Glu 1 5 10 15 Met Arg Glu Arg Leu Gly Thr Gly Gly Phe Gly Asn Val Cys Leu Tyr 20 25 30 Gln His Arg Glu Leu Asp Leu Lys Ile Ala Ile Lys Ser Cys Arg Leu 35 40 45 Glu Leu Ser Thr Lys Asn Arg Glu Arg Trp Cys His Glu Ile Gln Ile 50 55 60 Met Lys Lys Leu Asn His Ala Asn Val Val Lys Ala Cys Asp Val Pro 65 70 75 80 Glu Glu Leu Asn Ile Leu Ile His Asp Val Pro Leu Leu Ala Met Glu 85 90 95 Tyr Cys Ser Gly Gly Asp Leu Arg Lys Leu Leu Asn Lys Pro Glu Asn 100 105 110 Cys Cys Gly Leu Lys Glu Ser Gln Ile Leu Ser Leu Leu Ser Asp Ile 115 120 125 Gly Ser Gly Ile Arg Tyr Leu His Glu Asn Lys Ile Ile His Arg Asp 130 135 140 Leu Lys Pro Glu Asn Ile Val Leu Gln Asp Val Gly Gly Lys Ile Ile 145 150 155 160 His Lys Ile Ile Asp Leu Gly Tyr Ala Lys Asp Val Asp Gln Gly Glu 165 170 175 Leu Cys Thr Glu Phe Val Gly Thr Leu Gln Tyr Leu Ala Pro Glu Leu 180 185 190 Phe Glu Asn Lys Pro Tyr Thr Ala Thr Val Asp Tyr Trp Ser Phe Gly 195 200 205 Thr Met Val Phe Glu Cys Ile Ala Gly Tyr Arg Pro Phe Leu His His 210 215 220 Leu Gln Pro Phe Thr Trp His Glu Lys Ile Lys Lys Lys Asp Pro Lys 225 230 235 240 Cys Ile Phe Ala Cys Glu Glu Met Ser Gly Glu Val Arg Phe Ser Ser 245 250 255 His Leu Pro Gln Pro Asn Ser Leu Cys Ser Leu Val Val Glu Pro Met 260 265 270 Glu Asn Trp Leu Gln Leu Met Leu Asn Trp Asp Pro Gln Gln Arg Gly 275 280 285 Gly Pro Val Asp Leu Thr Leu Lys Gln Pro Arg Cys Phe Val Leu Met 290 295 300 Asp His Ile Leu Asn Leu Lys Ile Val His Ile Leu Asn Met Thr Ser 305 310 315 320 Ala Lys Ile Ile Ser Phe Leu Leu Pro Pro Asp Glu Ser Leu His Ser 325 330 335 Leu Gln Ser Arg Ile Glu Arg Glu Thr Gly Ile Asn Thr Gly Ser Gln 340 345 350 Glu Leu Leu Ser Glu Thr Gly Ile Ser Leu Asp Pro Arg Lys Pro Ala 355 360 365 Ser Gln Cys Val Leu Asp Gly Val Arg Gly Cys Asp Ser Tyr Met Val 370 375 380 Tyr Leu Phe Asp Lys Ser Lys Thr Val Tyr Glu Gly Pro Phe Ala Ser 385 390 395 400 Arg Ser Leu Ser Asp Cys Val Asn Tyr Ile Val Gln Asp Ser Lys Ile 405 410 415 Gln Leu Pro Ile Ile Gln Leu Arg Lys Val Trp Ala Glu Ala Val His 420 425 430 Tyr Val Ser Gly Leu Lys Glu Asp Tyr Ser Arg Leu Phe Gln Gly Gln 435 440 445 Arg Ala Ala Met Leu Ser Leu Leu Arg Tyr Asn Ala Asn Leu Thr Lys 450 455 460 Met Lys Asn Thr Leu Ile Ser Ala Ser Gln Gln Leu Lys Ala Lys Leu 465 470 475 480 Glu Phe Phe His Lys Ser Ile Gln Leu Asp Leu Glu Arg Tyr Ser Glu 485 490 495 Gln Met Thr Tyr Gly Ile Ser Ser Glu Lys Met Leu Lys Ala Trp Lys 500 505 510 Glu Met Glu Glu Lys Ala Ile His Tyr Ala Glu Val Gly Val Ile Gly 515 520 525 Tyr Leu Glu Asp Gln Ile Met Ser Leu His Ala Glu Ile Met Glu Leu 530 535 540 Gln Lys Ser Pro Tyr Arg Arg Gln Gly Asp Leu Met Glu Ser Leu Glu 545 550 555 560 Gln Arg Ala Ile Asp Leu Tyr Lys Gln Leu Lys His Arg Pro Ser Asp 565 570 575 His Ser Tyr Ser Asp Ser Thr Glu Met Val Lys Ile Ile Val His Thr 580 585 590 Val Gln Ser Gln Asp Arg Val Leu Lys Glu Leu Phe Gly His Leu Ser 595 600 605 Lys Leu Leu Gly Cys Lys Gln Lys Ile Ile Asp Leu Leu Pro Lys Val 610 615 620 Glu Val Ala Leu Ser Asn Ile Lys Glu Ala Asp Asn Thr Val Met Phe 625 630 635 640 Met Gln Gly Lys Arg Gln Lys Glu Ile Trp His Leu Leu Lys Ile Ala 645 650 655 Cys Thr Gln Ala Ala Ala Arg Ala Leu Val Gly Ala Ala Leu Glu Gly 660 665 670 Ala Val Ala Pro Gln Ala Ala Ala Trp Leu Pro Pro Ala Ala Ala Glu 675 680 685 His Asp His Ala Leu Ala Cys Val Val Ala Pro Gln Asp Gly Glu Ala 690 695 700 Ala Ala Gln Met Ile Glu Glu Asn Leu Asn Cys Leu Gly His Leu Ala 705 710 715 720 Ala Ile Ile His Glu Ala Asn Glu Glu Gln Gly Asn Ser Met Met Asn 725 730 735 Leu Asp Trp Ser Trp Leu Thr Glu 740 < 210 > 93 < 211 > 756 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 756 ) < 223 > Human constitutively active IKK beta ( PEST mutation ) P . 4015 without epitope tag < 400 > 93 Met Ser Trp Ser Pro Ser Leu Thr Thr Gln Thr Cys Gly Ala Trp Glu 1 5 10 15 Met Lys Glu Arg Leu Gly Thr Gly Gly Phe Gly Asn Val Ile Arg Trp 20 25 30 His Asn Gln Glu Thr Gly Glu Gln Ile Ala Ile Lys Gln Cys Arg Gln 35 40 45 Glu Leu Ser Pro Arg Asn Arg Glu Arg Trp Cys Leu Glu Ile Gln Ile 50 55 60 Met Arg Arg Leu Thr His Pro Asn Val Val Ala Ala Arg Asp Val Pro 65 70 75 80 Glu Gly Met Gln Asn Leu Ala Pro Asn Asp Leu Pro Leu Leu Ala Met 85 90 95 Glu Tyr Cys Gln Gly Gly Asp Leu Arg Lys Tyr Leu Asn Gln Phe Glu 100 105 110 Asn Cys Cys Gly Leu Arg Glu Gly Ala Ile Leu Thr Leu Leu Ser Asp 115 120 125 Ile Ala Ser Ala Leu Arg Tyr Leu His Glu Asn Arg Ile Ile His Arg 130 135 140 Asp Leu Lys Pro Glu Asn Ile Val Leu Gln Gln Gly Glu Gln Arg Leu 145 150 155 160 Ile His Lys Ile Ile Asp Leu Gly Tyr Ala Lys Glu Leu Asp Gln Gly 165 170 175 Glu Leu Cys Thr Glu Phe Val Gly Thr Leu Gln Tyr Leu Ala Pro Glu 180 185 190 Leu Leu Glu Gln Gln Lys Tyr Thr Val Thr Val Asp Tyr Trp Ser Phe 195 200 205 Gly Thr Leu Ala Phe Glu Cys Ile Thr Gly Phe Arg Pro Phe Leu Pro 210 215 220 Asn Trp Gln Pro Val Gln Trp His Ser Lys Val Arg Gln Lys Ser Glu 225 230 235 240 Val Asp Ile Val Val Ser Glu Asp Leu Asn Gly Thr Val Lys Phe Ser 245 250 255 Ser Ser Leu Pro Tyr Pro Asn Asn Leu Asn Ser Val Leu Ala Glu Arg 260 265 270 Leu Glu Lys Trp Leu Gln Leu Met Leu Met Trp His Pro Arg Gln Arg 275 280 285 Gly Thr Asp Pro Thr Tyr Gly Pro Asn Gly Cys Phe Lys Ala Leu Asp 290 295 300 Asp Ile Leu Asn Leu Lys Leu Val His Ile Leu Asn Met Val Thr Gly 305 310 315 320 Thr Ile His Thr Tyr Pro Val Thr Glu Asp Glu Ser Leu Gln Ser Leu 325 330 335 Lys Ala Arg Ile Gln Gln Asp Thr Gly Ile Pro Glu Glu Asp Gln Glu 340 345 350 Leu Leu Gln Glu Ala Gly Leu Ala Leu Ile Pro Asp Lys Pro Ala Thr 355 360 365 Gln Cys Ile Ser Asp Gly Lys Leu Asn Glu Gly His Thr Leu Asp Met 370 375 380 Asp Leu Val Phe Leu Phe Asp Asn Ser Lys Ile Thr Tyr Glu Thr Gln 385 390 395 400 Ile Ser Pro Arg Pro Gln Pro Glu Ser Val Ser Cys Ile Leu Gln Glu 405 410 415 Pro Lys Arg Asn Leu Ala Phe Phe Gln Leu Arg Lys Val Trp Gly Gln 420 425 430 Val Trp His Ser Ile Gln Thr Leu Lys Glu Asp Cys Asn Arg Leu Gln 435 440 445 Gln Gly Gln Arg Ala Ala Met Met Asn Leu Leu Arg Asn Asn Ser Cys 450 455 460 Leu Ser Lys Met Lys Asn Ser Met Ala Ser Met Ser Gln Gln Leu Lys 465 470 475 480 Ala Lys Leu Asp Phe Phe Lys Thr Ser Ile Gln Ile Asp Leu Glu Lys 485 490 495 Tyr Ser Glu Gln Thr Glu Phe Gly Ile Thr Ser Asp Lys Leu Leu Leu 500 505 510 Ala Trp Arg Glu Met Glu Gln Ala Val Glu Leu Cys Gly Arg Glu Asn 515 520 525 Glu Val Lys Leu Leu Val Glu Arg Met Met Ala Leu Gln Thr Asp Ile 530 535 540 Val Asp Leu Gln Arg Ser Pro Met Gly Arg Lys Gln Gly Gly Thr Leu 545 550 555 560 Asp Asp Leu Glu Glu Gln Ala Arg Glu Leu Tyr Arg Arg Leu Arg Glu 565 570 575 Lys Pro Arg Asp Gln Arg Thr Glu Gly Asp Ser Gln Glu Met Val Arg 580 585 590 Leu Leu Leu Gln Ala Ile Gln Ser Phe Glu Lys Lys Val Arg Val Ile 595 600 605 Tyr Thr Gln Leu Ser Lys Thr Val Val Cys Lys Gln Lys Ala Leu Glu 610 615 620 Leu Leu Pro Lys Val Glu Glu Val Val Ser Leu Met Asn Glu Asp Glu 625 630 635 640 Lys Thr Val Val Arg Leu Gln Glu Lys Arg Gln Lys Glu Leu Trp Asn 645 650 655 Leu Leu Lys Ile Ala Cys Ser Lys Val Arg Gly Pro Val Ala Gly Ala 660 665 670 Pro Asp Ala Met Asn Ala Ala Arg Leu Ala Gln Pro Gly Gln Leu Met 675 680 685 Ala Gln Pro Ala Thr Ala Ala Asn Ala Leu Pro Glu Pro Ala Lys Lys 690 695 700 Ala Glu Glu Leu Val Ala Glu Ala His Asn Leu Cys Thr Leu Leu Glu 705 710 715 720 Asn Ala Ile Gln Asp Thr Val Arg Glu Gln Asp Gln Ser Phe Thr Ala 725 730 735 Leu Asp Trp Ser Trp Leu Gln Thr Glu Glu Glu Glu His Ser Cys Leu 740 745 750 Glu Gln Ala Ser 755 < 210 > 94 < 211 > 756 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 756 ) < 223 > Human constitutively active IKK beta ( PEST mutation ) P . 4016 without epitope tag < 400 > 94 Met Ser Trp Ser Pro Ser Leu Thr Thr Gln Thr Cys Gly Ala Trp Glu 1 5 10 15 Met Lys Glu Arg Leu Gly Thr Gly Gly Phe Gly Asn Val Ile Arg Trp 20 25 30 His Asn Gln Glu Thr Gly Glu Gln Ile Ala Ile Lys Gln Cys Arg Gln 35 40 45 Glu Leu Ser Pro Arg Asn Arg Glu Arg Trp Cys Leu Glu Ile Gln Ile 50 55 60 Met Arg Arg Leu Thr His Pro Asn Val Val Ala Ala Arg Asp Val Pro 65 70 75 80 Glu Gly Met Gln Asn Leu Ala Pro Asn Asp Leu Pro Leu Leu Ala Met 85 90 95 Glu Tyr Cys Gln Gly Gly Asp Leu Arg Lys Tyr Leu Asn Gln Phe Glu 100 105 110 Asn Cys Cys Gly Leu Arg Glu Gly Ala Ile Leu Thr Leu Leu Ser Asp 115 120 125 Ile Ala Ser Ala Leu Arg Tyr Leu His Glu Asn Arg Ile Ile His Arg 130 135 140 Asp Leu Lys Pro Glu Asn Ile Val Leu Gln Gln Gly Glu Gln Arg Leu 145 150 155 160 Ile His Lys Ile Ile Asp Leu Gly Tyr Ala Lys Glu Leu Asp Gln Gly 165 170 175 Glu Leu Cys Thr Glu Phe Val Gly Thr Leu Gln Tyr Leu Ala Pro Glu 180 185 190 Leu Leu Glu Gln Gln Lys Tyr Thr Val Thr Val Asp Tyr Trp Ser Phe 195 200 205 Gly Thr Leu Ala Phe Glu Cys Ile Thr Gly Phe Arg Pro Phe Leu Pro 210 215 220 Asn Trp Gln Pro Val Gln Trp His Ser Lys Val Arg Gln Lys Ser Glu 225 230 235 240 Val Asp Ile Val Val Ser Glu Asp Leu Asn Gly Thr Val Lys Phe Ser 245 250 255 Ser Ser Leu Pro Tyr Pro Asn Asn Leu Asn Ser Val Leu Ala Glu Arg 260 265 270 Leu Glu Lys Trp Leu Gln Leu Met Leu Met Trp His Pro Arg Gln Arg 275 280 285 Gly Thr Asp Pro Thr Tyr Gly Pro Asn Gly Cys Phe Lys Ala Leu Asp 290 295 300 Asp Ile Leu Asn Leu Lys Leu Val His Ile Leu Asn Met Val Thr Gly 305 310 315 320 Thr Ile His Thr Tyr Pro Val Thr Glu Asp Glu Ser Leu Gln Ser Leu 325 330 335 Lys Ala Arg Ile Gln Gln Asp Thr Gly Ile Pro Glu Glu Asp Gln Glu 340 345 350 Leu Leu Gln Glu Ala Gly Leu Ala Leu Ile Pro Asp Lys Pro Ala Thr 355 360 365 Gln Cys Ile Ser Asp Gly Lys Leu Asn Glu Gly His Thr Leu Asp Met 370 375 380 Asp Leu Val Phe Leu Phe Asp Asn Ser Lys Ile Thr Tyr Glu Thr Gln 385 390 395 400 Ile Ser Pro Arg Pro Gln Pro Glu Ser Val Ser Cys Ile Leu Gln Glu 405 410 415 Pro Lys Arg Asn Leu Ala Phe Phe Gln Leu Arg Lys Val Trp Gly Gln 420 425 430 Val Trp His Ser Ile Gln Thr Leu Lys Glu Asp Cys Asn Arg Leu Gln 435 440 445 Gln Gly Gln Arg Ala Ala Met Met Asn Leu Leu Arg Asn Asn Ser Cys 450 455 460 Leu Ser Lys Met Lys Asn Ser Met Ala Ser Met Ser Gln Gln Leu Lys 465 470 475 480 Ala Lys Leu Asp Phe Phe Lys Thr Ser Ile Gln Ile Asp Leu Glu Lys 485 490 495 Tyr Ser Glu Gln Thr Glu Phe Gly Ile Thr Ser Asp Lys Leu Leu Leu 500 505 510 Ala Trp Arg Glu Met Glu Gln Ala Val Glu Leu Cys Gly Arg Glu Asn 515 520 525 Glu Val Lys Leu Leu Val Glu Arg Met Met Ala Leu Gln Thr Asp Ile 530 535 540 Val Asp Leu Gln Arg Ser Pro Met Gly Arg Lys Gln Gly Gly Thr Leu 545 550 555 560 Asp Asp Leu Glu Glu Gln Ala Arg Glu Leu Tyr Arg Arg Leu Arg Glu 565 570 575 Lys Pro Arg Asp Gln Arg Thr Glu Gly Asp Ser Gln Glu Met Val Arg 580 585 590 Leu Leu Leu Gln Ala Ile Gln Ser Phe Glu Lys Lys Val Arg Val Ile 595 600 605 Tyr Thr Gln Leu Ser Lys Thr Val Val Cys Lys Gln Lys Ala Leu Glu 610 615 620 Leu Leu Pro Lys Val Glu Glu Val Val Ser Leu Met Asn Glu Asp Glu 625 630 635 640 Lys Thr Val Val Arg Leu Gln Glu Lys Arg Gln Lys Glu Leu Trp Asn 645 650 655 Leu Leu Lys Ile Ala Cys Ser Lys Val Arg Gly Pro Val Ala Gly Ala 660 665 670 Pro Asp Ala Met Asn Ala Ala Arg Leu Ala Gln Pro Gly Gln Leu Met 675 680 685 Ala Gln Pro Ala Thr Ala Ala Asn Ala Leu Pro Glu Pro Ala Lys Lys 690 695 700 Ala Glu Glu Leu Val Ala Glu Ala His Asn Leu Cys Thr Leu Leu Glu 705 710 715 720 Asn Ala Ile Gln Asp Thr Val Arg Glu Gln Asp Gln Ser Phe Thr Ala 725 730 735 Leu Asp Trp Ser Trp Leu Gln Thr Glu Glu Glu Glu His Ser Cys Leu 740 745 750 Glu Gln Ala Ser 755 < 210 > 95 < 211 > 745 < 212 > PRT < 213 > Mus musculus < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 745 ) < 223 > Mouse constitutively active IKK alpha ( PEST mutation ) P . 4017 without epitope tag < 400 > 95 Met Glu Arg Pro Pro Gly Leu Arg Pro Gly Ala Gly Gly Pro Trp Glu 1 5 10 15 Met Arg Glu Arg Leu Gly Thr Gly Gly Phe Gly Asn Val Ser Leu Tyr 20 25 30 Gln His Arg Glu Leu Asp Leu Lys Ile Ala Ile Lys Ser Cys Arg Leu 35 40 45 Glu Leu Ser Ser Lys Asn Arg Glu Arg Trp Cys His Glu Ile Gln Ile 50 55 60 Met Lys Lys Leu Asp His Ala Asn Val Val Lys Ala Cys Asp Val Pro 65 70 75 80 Glu Glu Leu Asn Phe Leu Ile Asn Asp Val Pro Leu Leu Ala Met Glu 85 90 95 Tyr Cys Ser Gly Gly Asp Leu Arg Lys Leu Leu Asn Lys Pro Glu Asn 100 105 110 Cys Cys Gly Leu Lys Glu Ser Gln Ile Leu Ser Leu Leu Ser Asp Ile 115 120 125 Gly Ser Gly Ile Arg Tyr Leu His Glu Asn Lys Ile Ile His Arg Asp 130 135 140 Leu Lys Pro Glu Asn Ile Val Leu Gln Asp Val Gly Gly Lys Thr Ile 145 150 155 160 His Lys Ile Ile Asp Leu Gly Tyr Ala Lys Asp Val Asp Gln Gly Glu 165 170 175 Leu Cys Thr Glu Phe Val Gly Thr Leu Gln Tyr Leu Ala Pro Glu Leu 180 185 190 Phe Glu Asn Lys Pro Tyr Thr Ala Thr Val Asp Tyr Trp Ser Phe Gly 195 200 205 Thr Met Val Phe Glu Cys Ile Ala Gly Tyr Arg Pro Phe Leu His His 210 215 220 Leu Gln Pro Phe Thr Trp His Glu Lys Ile Lys Lys Lys Asp Pro Lys 225 230 235 240 Cys Ile Phe Ala Cys Glu Glu Met Thr Gly Glu Val Arg Phe Ser Ser 245 250 255 His Leu Pro Gln Pro Asn Ser Leu Cys Ser Leu Ile Val Glu Pro Met 260 265 270 Glu Ser Trp Leu Gln Leu Met Leu Asn Trp Asp Pro Gln Gln Arg Gly 275 280 285 Gly Pro Ile Asp Leu Thr Leu Lys Gln Pro Arg Cys Phe Ala Leu Met 290 295 300 Asp His Ile Leu Asn Leu Lys Ile Val His Ile Leu Asn Met Thr Ser 305 310 315 320 Ala Lys Ile Ile Ser Phe Leu Leu Pro Cys Asp Glu Ser Leu His Ser 325 330 335 Leu Gln Ser Arg Ile Glu Arg Glu Thr Gly Ile Asn Thr Gly Ser Gln 340 345 350 Glu Leu Leu Ser Glu Thr Gly Ile Ser Leu Asp Pro Arg Lys Pro Ala 355 360 365 Ser Gln Cys Val Leu Asp Gly Val Arg Gly Cys Asp Ser Tyr Met Val 370 375 380 Tyr Leu Phe Asp Lys Ser Lys Thr Val Tyr Glu Gly Pro Phe Ala Ser 385 390 395 400 Arg Ser Leu Ser Asp Cys Val Asn Tyr Ile Val Gln Asp Ser Lys Ile 405 410 415 Gln Leu Pro Ile Ile Gln Leu Arg Lys Val Trp Ala Glu Ala Val His 420 425 430 Tyr Val Ser Gly Leu Lys Glu Asp Tyr Ser Arg Leu Phe Gln Gly Gln 435 440 445 Arg Ala Ala Met Leu Ser Leu Leu Arg Tyr Asn Ala Asn Leu Thr Lys 450 455 460 Met Lys Asn Thr Leu Ile Ser Ala Ser Gln Gln Leu Lys Ala Lys Leu 465 470 475 480 Glu Phe Phe Arg Lys Ser Ile Gln Leu Asp Leu Glu Arg Tyr Ser Glu 485 490 495 Gln Met Thr Tyr Gly Ile Ser Ser Glu Lys Met Leu Lys Ala Trp Lys 500 505 510 Glu Met Glu Glu Lys Ala Ile His Tyr Ser Glu Val Gly Val Ile Gly 515 520 525 Tyr Leu Glu Asp Gln Ile Met Ser Leu His Thr Glu Ile Met Glu Leu 530 535 540 Gln Lys Ser Pro Tyr Gly Arg Arg Gln Gly Asp Leu Met Glu Ser Leu 545 550 555 560 Glu Gln Arg Ala Ile Asp Leu Tyr Lys Gln Leu Lys His Arg Pro Pro 565 570 575 Asp His Leu Tyr Ser Asp Ser Thr Glu Met Val Lys Ile Ile Val His 580 585 590 Thr Val Gln Ser Gln Asp Arg Val Leu Lys Glu Leu Phe Gly His Leu 595 600 605 Ser Lys Leu Leu Gly Cys Lys Gln Lys Ile Ile Asp Leu Leu Pro Lys 610 615 620 Val Glu Val Ala Leu Ser Asn Ile Lys Glu Ala Asp Asn Thr Val Met 625 630 635 640 Phe Met Gln Gly Lys Arg Gln Lys Glu Ile Trp His Leu Leu Lys Ile 645 650 655 Ala Cys Thr Gln Ala Ala Ala Arg Ala Leu Val Gly Ala Ala Leu Glu 660 665 670 Gly Ala Val Ala Pro Pro Val Ala Ala Trp Leu Pro Pro Ala Leu Ala 675 680 685 Asp Arg Glu His Pro Leu Thr Cys Val Val Ala Pro Gln Asp Gly Glu 690 695 700 Ala Leu Ala Gln Met Ile Glu Glu Asn Leu Asn Cys Leu Gly His Leu 705 710 715 720 Ala Ala Ile Ile Arg Glu Ala Asn Glu Asp Gln Ser Ser Ser Leu Met 725 730 735 Ser Leu Asp Trp Ser Trp Leu Ala Glu 740 745 < 210 > 96 < 211 > 745 < 212 > PRT < 213 > Mus musculus < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 745 ) < 223 > Mouse constitutively active IKK alpha ( PEST mutation ) P . 4018 without epitope tag < 400 > 96 Met Glu Arg Pro Pro Gly Leu Arg Pro Gly Ala Gly Gly Pro Trp Glu 1 5 10 15 Met Arg Glu Arg Leu Gly Thr Gly Gly Phe Gly Asn Val Ser Leu Tyr 20 25 30 Gln His Arg Glu Leu Asp Leu Lys Ile Ala Ile Lys Ser Cys Arg Leu 35 40 45 Glu Leu Ser Ser Lys Asn Arg Glu Arg Trp Cys His Glu Ile Gln Ile 50 55 60 Met Lys Lys Leu Asp His Ala Asn Val Val Lys Ala Cys Asp Val Pro 65 70 75 80 Glu Glu Leu Asn Phe Leu Ile Asn Asp Val Pro Leu Leu Ala Met Glu 85 90 95 Tyr Cys Ser Gly Gly Asp Leu Arg Lys Leu Leu Asn Lys Pro Glu Asn 100 105 110 Cys Cys Gly Leu Lys Glu Ser Gln Ile Leu Ser Leu Leu Ser Asp Ile 115 120 125 Gly Ser Gly Ile Arg Tyr Leu His Glu Asn Lys Ile Ile His Arg Asp 130 135 140 Leu Lys Pro Glu Asn Ile Val Leu Gln Asp Val Gly Gly Lys Thr Ile 145 150 155 160 His Lys Ile Ile Asp Leu Gly Tyr Ala Lys Asp Val Asp Gln Gly Glu 165 170 175 Leu Cys Thr Glu Phe Val Gly Thr Leu Gln Tyr Leu Ala Pro Glu Leu 180 185 190 Phe Glu Asn Lys Pro Tyr Thr Ala Thr Val Asp Tyr Trp Ser Phe Gly 195 200 205 Thr Met Val Phe Glu Cys Ile Ala Gly Tyr Arg Pro Phe Leu His His 210 215 220 Leu Gln Pro Phe Thr Trp His Glu Lys Ile Lys Lys Lys Asp Pro Lys 225 230 235 240 Cys Ile Phe Ala Cys Glu Glu Met Thr Gly Glu Val Arg Phe Ser Ser 245 250 255 His Leu Pro Gln Pro Asn Ser Leu Cys Ser Leu Ile Val Glu Pro Met 260 265 270 Glu Ser Trp Leu Gln Leu Met Leu Asn Trp Asp Pro Gln Gln Arg Gly 275 280 285 Gly Pro Ile Asp Leu Thr Leu Lys Gln Pro Arg Cys Phe Ala Leu Met 290 295 300 Asp His Ile Leu Asn Leu Lys Ile Val His Ile Leu Asn Met Thr Ser 305 310 315 320 Ala Lys Ile Ile Ser Phe Leu Leu Pro Cys Asp Glu Ser Leu His Ser 325 330 335 Leu Gln Ser Arg Ile Glu Arg Glu Thr Gly Ile Asn Thr Gly Ser Gln 340 345 350 Glu Leu Leu Ser Glu Thr Gly Ile Ser Leu Asp Pro Arg Lys Pro Ala 355 360 365 Ser Gln Cys Val Leu Asp Gly Val Arg Gly Cys Asp Ser Tyr Met Val 370 375 380 Tyr Leu Phe Asp Lys Ser Lys Thr Val Tyr Glu Gly Pro Phe Ala Ser 385 390 395 400 Arg Ser Leu Ser Asp Cys Val Asn Tyr Ile Val Gln Asp Ser Lys Ile 405 410 415 Gln Leu Pro Ile Ile Gln Leu Arg Lys Val Trp Ala Glu Ala Val His 420 425 430 Tyr Val Ser Gly Leu Lys Glu Asp Tyr Ser Arg Leu Phe Gln Gly Gln 435 440 445 Arg Ala Ala Met Leu Ser Leu Leu Arg Tyr Asn Ala Asn Leu Thr Lys 450 455 460 Met Lys Asn Thr Leu Ile Ser Ala Ser Gln Gln Leu Lys Ala Lys Leu 465 470 475 480 Glu Phe Phe Arg Lys Ser Ile Gln Leu Asp Leu Glu Arg Tyr Ser Glu 485 490 495 Gln Met Thr Tyr Gly Ile Ser Ser Glu Lys Met Leu Lys Ala Trp Lys 500 505 510 Glu Met Glu Glu Lys Ala Ile His Tyr Ser Glu Val Gly Val Ile Gly 515 520 525 Tyr Leu Glu Asp Gln Ile Met Ser Leu His Thr Glu Ile Met Glu Leu 530 535 540 Gln Lys Ser Pro Tyr Gly Arg Arg Gln Gly Asp Leu Met Glu Ser Leu 545 550 555 560 Glu Gln Arg Ala Ile Asp Leu Tyr Lys Gln Leu Lys His Arg Pro Pro 565 570 575 Asp His Leu Tyr Ser Asp Ser Thr Glu Met Val Lys Ile Ile Val His 580 585 590 Thr Val Gln Ser Gln Asp Arg Val Leu Lys Glu Leu Phe Gly His Leu 595 600 605 Ser Lys Leu Leu Gly Cys Lys Gln Lys Ile Ile Asp Leu Leu Pro Lys 610 615 620 Val Glu Val Ala Leu Ser Asn Ile Lys Glu Ala Asp Asn Thr Val Met 625 630 635 640 Phe Met Gln Gly Lys Arg Gln Lys Glu Ile Trp His Leu Leu Lys Ile 645 650 655 Ala Cys Thr Gln Ala Ala Ala Arg Ala Leu Val Gly Ala Ala Leu Glu 660 665 670 Gly Ala Val Ala Pro Pro Val Ala Ala Trp Leu Pro Pro Ala Leu Ala 675 680 685 Asp Arg Glu His Pro Leu Thr Cys Val Val Ala Pro Gln Asp Gly Glu 690 695 700 Ala Leu Ala Gln Met Ile Glu Glu Asn Leu Asn Cys Leu Gly His Leu 705 710 715 720 Ala Ala Ile Ile Arg Glu Ala Asn Glu Asp Gln Ser Ser Ser Leu Met 725 730 735 Ser Leu Asp Trp Ser Trp Leu Ala Glu 740 745 < 210 > 97 < 211 > 757 < 212 > PRT < 213 > Mus musculus < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 757 ) < 223 > Mouse constitutively active IKK beta ( PEST mutation ) P . 4019 without epitope tag < 400 > 97 Met Ser Trp Ser Pro Ser Leu Pro Thr Gln Thr Cys Gly Ala Trp Glu 1 5 10 15 Met Lys Glu Arg Leu Gly Thr Gly Gly Phe Gly Asn Val Ile Arg Trp 20 25 30 His Asn Gln Ala Thr Gly Glu Gln Ile Ala Ile Lys Gln Cys Arg Gln 35 40 45 Glu Leu Ser Pro Lys Asn Arg Asn Arg Trp Cys Leu Glu Ile Gln Ile 50 55 60 Met Arg Arg Leu Asn His Pro Asn Val Val Ala Ala Arg Asp Val Pro 65 70 75 80 Glu Gly Met Gln Asn Leu Ala Pro Asn Asp Leu Pro Leu Leu Ala Met 85 90 95 Glu Tyr Cys Gln Gly Gly Asp Leu Arg Arg Tyr Leu Asn Gln Phe Glu 100 105 110 Asn Cys Cys Gly Leu Arg Glu Gly Ala Val Leu Thr Leu Leu Ser Asp 115 120 125 Ile Ala Ser Ala Leu Arg Tyr Leu His Glu Asn Arg Ile Ile His Arg 130 135 140 Asp Leu Lys Pro Glu Asn Ile Val Leu Gln Gln Gly Glu Lys Arg Leu 145 150 155 160 Ile His Lys Ile Ile Asp Leu Gly Tyr Ala Lys Glu Leu Asp Gln Gly 165 170 175 Glu Leu Cys Thr Glu Phe Val Gly Thr Leu Gln Tyr Leu Ala Pro Glu 180 185 190 Leu Leu Glu Gln Gln Lys Tyr Thr Val Thr Val Asp Tyr Trp Ser Phe 195 200 205 Gly Thr Leu Ala Phe Glu Cys Ile Thr Gly Phe Arg Pro Phe Leu Pro 210 215 220 Asn Trp Gln Pro Val Gln Trp His Ser Lys Val Arg Gln Lys Ser Glu 225 230 235 240 Val Asp Ile Val Val Ser Glu Asp Leu Asn Gly Ala Val Lys Phe Ser 245 250 255 Ser Ser Leu Pro Phe Pro Asn Asn Leu Asn Ser Val Leu Ala Glu Arg 260 265 270 Leu Glu Lys Trp Leu Gln Leu Met Leu Met Trp His Pro Arg Gln Arg 275 280 285 Gly Thr Asp Pro Gln Tyr Gly Pro Asn Gly Cys Phe Arg Ala Leu Asp 290 295 300 Asp Ile Leu Asn Leu Lys Leu Val His Val Leu Asn Met Val Thr Gly 305 310 315 320 Thr Val His Thr Tyr Pro Val Thr Glu Asp Glu Ser Leu Gln Ser Leu 325 330 335 Lys Thr Arg Ile Gln Glu Asn Thr Gly Ile Leu Glu Thr Asp Gln Glu 340 345 350 Leu Leu Gln Lys Ala Gly Leu Val Leu Leu Pro Asp Lys Pro Ala Thr 355 360 365 Gln Cys Ile Ser Asp Ser Lys Thr Asn Glu Gly Leu Thr Leu Asp Met 370 375 380 Asp Leu Val Phe Leu Leu Asp Asn Ser Lys Ile Asn Tyr Glu Thr Gln 385 390 395 400 Ile Thr Pro Arg Pro Pro Pro Glu Ser Val Ser Cys Ile Leu Gln Glu 405 410 415 Pro Lys Arg Asn Leu Ser Phe Phe Gln Leu Arg Lys Val Trp Gly Gln 420 425 430 Val Trp His Ser Ile Gln Thr Leu Lys Glu Asp Cys Asn Arg Leu Gln 435 440 445 Gln Gly Gln Arg Ala Ala Met Met Ser Leu Leu Arg Asn Asn Ser Cys 450 455 460 Leu Ser Lys Met Lys Asn Ala Met Ala Ser Thr Ala Gln Gln Leu Lys 465 470 475 480 Ala Lys Leu Asp Phe Phe Lys Thr Ser Ile Gln Ile Asp Leu Glu Lys 485 490 495 Tyr Lys Glu Gln Thr Glu Phe Gly Ile Thr Ser Asp Lys Leu Leu Leu 500 505 510 Ala Trp Arg Glu Met Glu Gln Ala Val Glu Gln Cys Gly Arg Glu Asn 515 520 525 Asp Val Lys His Leu Val Glu Arg Met Met Ala Leu Gln Thr Asp Ile 530 535 540 Val Asp Leu Gln Arg Ser Pro Met Gly Arg Lys Gln Gly Gly Thr Leu 545 550 555 560 Asp Asp Leu Glu Glu Gln Ala Arg Glu Leu Tyr Arg Lys Leu Arg Glu 565 570 575 Lys Pro Arg Asp Gln Arg Thr Glu Gly Asp Ser Gln Glu Met Val Arg 580 585 590 Leu Leu Leu Gln Ala Ile Gln Ser Phe Glu Lys Lys Val Arg Val Ile 595 600 605 Tyr Thr Gln Leu Ser Lys Thr Val Val Cys Lys Gln Lys Ala Leu Glu 610 615 620 Leu Leu Pro Lys Val Glu Glu Val Val Ser Leu Met Asn Glu Asp Glu 625 630 635 640 Arg Thr Val Val Arg Leu Gln Glu Lys Arg Gln Lys Glu Leu Trp Asn 645 650 655 Leu Leu Lys Ile Ala Cys Ser Lys Val Arg Gly Pro Val Ala Gly Ala 660 665 670 Pro Asp Ala Met Asn Val Ala Arg Leu Ala His Pro Gly Gln Leu Met 675 680 685 Ala Gln Pro Ala Ser Ala Cys Asp Ala Leu Pro Glu Ser Asp Lys Lys 690 695 700 Ala Glu Glu Leu Val Ala Glu Ala His Ala Leu Cys Ser Arg Leu Glu 705 710 715 720 Ser Ala Leu Gln Asp Thr Val Lys Glu Gln Asp Arg Ser Phe Thr Thr 725 730 735 Leu Asp Trp Ser Trp Leu Gln Met Glu Asp Glu Glu Arg Cys Ser Leu 740 745 750 Glu Gln Ala Cys Asp 755 < 210 > 98 < 211 > 757 < 212 > PRT < 213 > Mus musculus < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 757 ) < 223 > Mouse constitutively active IKK beta ( PEST mutation ) P . 4020 without epitope tag < 400 > 98 Met Ser Trp Ser Pro Ser Leu Pro Thr Gln Thr Cys Gly Ala Trp Glu 1 5 10 15 Met Lys Glu Arg Leu Gly Thr Gly Gly Phe Gly Asn Val Ile Arg Trp 20 25 30 His Asn Gln Ala Thr Gly Glu Gln Ile Ala Ile Lys Gln Cys Arg Gln 35 40 45 Glu Leu Ser Pro Lys Asn Arg Asn Arg Trp Cys Leu Glu Ile Gln Ile 50 55 60 Met Arg Arg Leu Asn His Pro Asn Val Val Ala Ala Arg Asp Val Pro 65 70 75 80 Glu Gly Met Gln Asn Leu Ala Pro Asn Asp Leu Pro Leu Leu Ala Met 85 90 95 Glu Tyr Cys Gln Gly Gly Asp Leu Arg Arg Tyr Leu Asn Gln Phe Glu 100 105 110 Asn Cys Cys Gly Leu Arg Glu Gly Ala Val Leu Thr Leu Leu Ser Asp 115 120 125 Ile Ala Ser Ala Leu Arg Tyr Leu His Glu Asn Arg Ile Ile His Arg 130 135 140 Asp Leu Lys Pro Glu Asn Ile Val Leu Gln Gln Gly Glu Lys Arg Leu 145 150 155 160 Ile His Lys Ile Ile Asp Leu Gly Tyr Ala Lys Glu Leu Asp Gln Gly 165 170 175 Glu Leu Cys Thr Glu Phe Val Gly Thr Leu Gln Tyr Leu Ala Pro Glu 180 185 190 Leu Leu Glu Gln Gln Lys Tyr Thr Val Thr Val Asp Tyr Trp Ser Phe 195 200 205 Gly Thr Leu Ala Phe Glu Cys Ile Thr Gly Phe Arg Pro Phe Leu Pro 210 215 220 Asn Trp Gln Pro Val Gln Trp His Ser Lys Val Arg Gln Lys Ser Glu 225 230 235 240 Val Asp Ile Val Val Ser Glu Asp Leu Asn Gly Ala Val Lys Phe Ser 245 250 255 Ser Ser Leu Pro Phe Pro Asn Asn Leu Asn Ser Val Leu Ala Glu Arg 260 265 270 Leu Glu Lys Trp Leu Gln Leu Met Leu Met Trp His Pro Arg Gln Arg 275 280 285 Gly Thr Asp Pro Gln Tyr Gly Pro Asn Gly Cys Phe Arg Ala Leu Asp 290 295 300 Asp Ile Leu Asn Leu Lys Leu Val His Val Leu Asn Met Val Thr Gly 305 310 315 320 Thr Val His Thr Tyr Pro Val Thr Glu Asp Glu Ser Leu Gln Ser Leu 325 330 335 Lys Thr Arg Ile Gln Glu Asn Thr Gly Ile Leu Glu Thr Asp Gln Glu 340 345 350 Leu Leu Gln Lys Ala Gly Leu Val Leu Leu Pro Asp Lys Pro Ala Thr 355 360 365 Gln Cys Ile Ser Asp Ser Lys Thr Asn Glu Gly Leu Thr Leu Asp Met 370 375 380 Asp Leu Val Phe Leu Leu Asp Asn Ser Lys Ile Asn Tyr Glu Thr Gln 385 390 395 400 Ile Thr Pro Arg Pro Pro Pro Glu Ser Val Ser Cys Ile Leu Gln Glu 405 410 415 Pro Lys Arg Asn Leu Ser Phe Phe Gln Leu Arg Lys Val Trp Gly Gln 420 425 430 Val Trp His Ser Ile Gln Thr Leu Lys Glu Asp Cys Asn Arg Leu Gln 435 440 445 Gln Gly Gln Arg Ala Ala Met Met Ser Leu Leu Arg Asn Asn Ser Cys 450 455 460 Leu Ser Lys Met Lys Asn Ala Met Ala Ser Thr Ala Gln Gln Leu Lys 465 470 475 480 Ala Lys Leu Asp Phe Phe Lys Thr Ser Ile Gln Ile Asp Leu Glu Lys 485 490 495 Tyr Lys Glu Gln Thr Glu Phe Gly Ile Thr Ser Asp Lys Leu Leu Leu 500 505 510 Ala Trp Arg Glu Met Glu Gln Ala Val Glu Gln Cys Gly Arg Glu Asn 515 520 525 Asp Val Lys His Leu Val Glu Arg Met Met Ala Leu Gln Thr Asp Ile 530 535 540 Val Asp Leu Gln Arg Ser Pro Met Gly Arg Lys Gln Gly Gly Thr Leu 545 550 555 560 Asp Asp Leu Glu Glu Gln Ala Arg Glu Leu Tyr Arg Lys Leu Arg Glu 565 570 575 Lys Pro Arg Asp Gln Arg Thr Glu Gly Asp Ser Gln Glu Met Val Arg 580 585 590 Leu Leu Leu Gln Ala Ile Gln Ser Phe Glu Lys Lys Val Arg Val Ile 595 600 605 Tyr Thr Gln Leu Ser Lys Thr Val Val Cys Lys Gln Lys Ala Leu Glu 610 615 620 Leu Leu Pro Lys Val Glu Glu Val Val Ser Leu Met Asn Glu Asp Glu 625 630 635 640 Arg Thr Val Val Arg Leu Gln Glu Lys Arg Gln Lys Glu Leu Trp Asn 645 650 655 Leu Leu Lys Ile Ala Cys Ser Lys Val Arg Gly Pro Val Ala Gly Ala 660 665 670 Pro Asp Ala Met Asn Val Ala Arg Leu Ala His Pro Gly Gln Leu Met 675 680 685 Ala Gln Pro Ala Ser Ala Cys Asp Ala Leu Pro Glu Ser Asp Lys Lys 690 695 700 Ala Glu Glu Leu Val Ala Glu Ala His Ala Leu Cys Ser Arg Leu Glu 705 710 715 720 Ser Ala Leu Gln Asp Thr Val Lys Glu Gln Asp Arg Ser Phe Thr Thr 725 730 735 Leu Asp Trp Ser Trp Leu Gln Met Glu Asp Glu Glu Arg Cys Ser Leu 740 745 750 Glu Gln Ala Cys Asp 755 < 210 > 99 < 211 > 585 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : huRIPK 1 ( 1 - 555 ) . IZ . TM ; TH 1021 without epitope tag < 400 > 99 Met Gln Pro Asp Met Ser Leu Asn Val Ile Lys Met Lys Ser Ser Asp 1 5 10 15 Phe Leu Glu Ser Ala Glu Leu Asp Ser Gly Gly Phe Gly Lys Val Ser 20 25 30 Leu Cys Phe His Arg Thr Gln Gly Leu Met Ile Met Lys Thr Val Tyr 35 40 45 Lys Gly Pro Asn Cys Ile Glu His Asn Glu Ala Leu Leu Glu Glu Ala 50 55 60 Lys Met Met Asn Arg Leu Arg His Ser Arg Val Val Lys Leu Leu Gly 65 70 75 80 Val Ile Ile Glu Glu Gly Lys Tyr Ser Leu Val Met Glu Tyr Met Glu 85 90 95 Lys Gly Asn Leu Met His Val Leu Lys Ala Glu Met Ser Thr Pro Leu 100 105 110 Ser Val Lys Gly Arg Ile Ile Leu Glu Ile Ile Glu Gly Met Cys Tyr 115 120 125 Leu His Gly Lys Gly Val Ile His Lys Asp Leu Lys Pro Glu Asn Ile 130 135 140 Leu Val Asp Asn Asp Phe His Ile Lys Ile Ala Asp Leu Gly Leu Ala 145 150 155 160 Ser Phe Lys Met Trp Ser Lys Leu Asn Asn Glu Glu His Asn Glu Leu 165 170 175 Arg Glu Val Asp Gly Thr Ala Lys Lys Asn Gly Gly Thr Leu Tyr Tyr 180 185 190 Met Ala Pro Glu His Leu Asn Asp Val Asn Ala Lys Pro Thr Glu Lys 195 200 205 Ser Asp Val Tyr Ser Phe Ala Val Val Leu Trp Ala Ile Phe Ala Asn 210 215 220 Lys Glu Pro Tyr Glu Asn Ala Ile Cys Glu Gln Gln Leu Ile Met Cys 225 230 235 240 Ile Lys Ser Gly Asn Arg Pro Asp Val Asp Asp Ile Thr Glu Tyr Cys 245 250 255 Pro Arg Glu Ile Ile Ser Leu Met Lys Leu Cys Trp Glu Ala Asn Pro 260 265 270 Glu Ala Arg Pro Thr Phe Pro Gly Ile Glu Glu Lys Phe Arg Pro Phe 275 280 285 Tyr Leu Ser Gln Leu Glu Glu Ser Val Glu Glu Asp Val Lys Ser Leu 290 295 300 Lys Lys Glu Tyr Ser Asn Glu Asn Ala Val Val Lys Arg Met Gln Ser 305 310 315 320 Leu Gln Leu Asp Cys Val Ala Val Pro Ser Ser Arg Ser Asn Ser Ala 325 330 335 Thr Glu Gln Pro Gly Ser Leu His Ser Ser Gln Gly Leu Gly Met Gly 340 345 350 Pro Val Glu Glu Ser Trp Phe Ala Pro Ser Leu Glu His Pro Gln Glu 355 360 365 Glu Asn Glu Pro Ser Leu Gln Ser Lys Leu Gln Asp Glu Ala Asn Tyr 370 375 380 His Leu Tyr Gly Ser Arg Met Asp Arg Gln Thr Lys Gln Gln Pro Arg 385 390 395 400 Gln Asn Val Ala Tyr Asn Arg Glu Glu Glu Arg Arg Arg Arg Val Ser 405 410 415 His Asp Pro Phe Ala Gln Gln Arg Pro Tyr Glu Asn Phe Gln Asn Thr 420 425 430 Glu Gly Lys Gly Thr Ala Tyr Ser Ser Ala Ala Ser His Gly Asn Ala 435 440 445 Val His Gln Pro Ser Gly Leu Thr Ser Gln Pro Gln Val Leu Tyr Gln 450 455 460 Asn Asn Gly Leu Tyr Ser Ser His Gly Phe Gly Thr Arg Pro Leu Asp 465 470 475 480 Pro Gly Thr Ala Gly Pro Arg Val Trp Tyr Arg Pro Ile Pro Ser His 485 490 495 Met Pro Ser Leu His Asn Ile Pro Val Pro Glu Thr Asn Tyr Leu Gly 500 505 510 Asn Thr Pro Thr Met Pro Phe Ser Ser Leu Pro Pro Thr Asp Glu Ser 515 520 525 Ile Lys Tyr Thr Ile Tyr Asn Ser Thr Gly Ile Gln Ile Gly Ala Tyr 530 535 540 Asn Tyr Met Glu Ile Gly Gly Thr Ser Ser Ser Gly Gly Ile Lys Lys 545 550 555 560 Glu Ile Glu Ala Ile Lys Lys Glu Gln Glu Ala Ile Lys Lys Lys Ile 565 570 575 Glu Ala Ile Glu Lys Glu Ile Glu Ala 580 585 < 210 > 100 < 211 > 609 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : huRIPK 1 ( 1 - 555 ) . EE . DM ; TH 1022 without epitope tag < 400 > 100 Met Gln Pro Asp Met Ser Leu Asn Val Ile Lys Met Lys Ser Ser Asp 1 5 10 15 Phe Leu Glu Ser Ala Glu Leu Asp Ser Gly Gly Phe Gly Lys Val Ser 20 25 30 Leu Cys Phe His Arg Thr Gln Gly Leu Met Ile Met Lys Thr Val Tyr 35 40 45 Lys Gly Pro Asn Cys Ile Glu His Asn Glu Ala Leu Leu Glu Glu Ala 50 55 60 Lys Met Met Asn Arg Leu Arg His Ser Arg Val Val Lys Leu Leu Gly 65 70 75 80 Val Ile Ile Glu Glu Gly Lys Tyr Ser Leu Val Met Glu Tyr Met Glu 85 90 95 Lys Gly Asn Leu Met His Val Leu Lys Ala Glu Met Ser Thr Pro Leu 100 105 110 Ser Val Lys Gly Arg Ile Ile Leu Glu Ile Ile Glu Gly Met Cys Tyr 115 120 125 Leu His Gly Lys Gly Val Ile His Lys Asp Leu Lys Pro Glu Asn Ile 130 135 140 Leu Val Asp Asn Asp Phe His Ile Lys Ile Ala Asp Leu Gly Leu Ala 145 150 155 160 Ser Phe Lys Met Trp Ser Lys Leu Asn Asn Glu Glu His Asn Glu Leu 165 170 175 Arg Glu Val Asp Gly Thr Ala Lys Lys Asn Gly Gly Thr Leu Tyr Tyr 180 185 190 Met Ala Pro Glu His Leu Asn Asp Val Asn Ala Lys Pro Thr Glu Lys 195 200 205 Ser Asp Val Tyr Ser Phe Ala Val Val Leu Trp Ala Ile Phe Ala Asn 210 215 220 Lys Glu Pro Tyr Glu Asn Ala Ile Cys Glu Gln Gln Leu Ile Met Cys 225 230 235 240 Ile Lys Ser Gly Asn Arg Pro Asp Val Asp Asp Ile Thr Glu Tyr Cys 245 250 255 Pro Arg Glu Ile Ile Ser Leu Met Lys Leu Cys Trp Glu Ala Asn Pro 260 265 270 Glu Ala Arg Pro Thr Phe Pro Gly Ile Glu Glu Lys Phe Arg Pro Phe 275 280 285 Tyr Leu Ser Gln Leu Glu Glu Ser Val Glu Glu Asp Val Lys Ser Leu 290 295 300 Lys Lys Glu Tyr Ser Asn Glu Asn Ala Val Val Lys Arg Met Gln Ser 305 310 315 320 Leu Gln Leu Asp Cys Val Ala Val Pro Ser Ser Arg Ser Asn Ser Ala 325 330 335 Thr Glu Gln Pro Gly Ser Leu His Ser Ser Gln Gly Leu Gly Met Gly 340 345 350 Pro Val Glu Glu Ser Trp Phe Ala Pro Ser Leu Glu His Pro Gln Glu 355 360 365 Glu Asn Glu Pro Ser Leu Gln Ser Lys Leu Gln Asp Glu Ala Asn Tyr 370 375 380 His Leu Tyr Gly Ser Arg Met Asp Arg Gln Thr Lys Gln Gln Pro Arg 385 390 395 400 Gln Asn Val Ala Tyr Asn Arg Glu Glu Glu Arg Arg Arg Arg Val Ser 405 410 415 His Asp Pro Phe Ala Gln Gln Arg Pro Tyr Glu Asn Phe Gln Asn Thr 420 425 430 Glu Gly Lys Gly Thr Ala Tyr Ser Ser Ala Ala Ser His Gly Asn Ala 435 440 445 Val His Gln Pro Ser Gly Leu Thr Ser Gln Pro Gln Val Leu Tyr Gln 450 455 460 Asn Asn Gly Leu Tyr Ser Ser His Gly Phe Gly Thr Arg Pro Leu Asp 465 470 475 480 Pro Gly Thr Ala Gly Pro Arg Val Trp Tyr Arg Pro Ile Pro Ser His 485 490 495 Met Pro Ser Leu His Asn Ile Pro Val Pro Glu Thr Asn Tyr Leu Gly 500 505 510 Asn Thr Pro Thr Met Pro Phe Ser Ser Leu Pro Pro Thr Asp Glu Ser 515 520 525 Ile Lys Tyr Thr Ile Tyr Asn Ser Thr Gly Ile Gln Ile Gly Ala Tyr 530 535 540 Asn Tyr Met Glu Ile Gly Gly Thr Ser Ser Ser Gly Ser Asp Gly Ser 545 550 555 560 Gly Ser Gly Ser Gly Ser Ile Thr Ile Arg Ala Ala Phe Leu Glu Lys 565 570 575 Glu Asn Thr Ala Leu Arg Thr Glu Ile Ala Glu Leu Glu Lys Glu Val 580 585 590 Gly Arg Cys Glu Asn Ile Val Ser Lys Tyr Glu Thr Arg Tyr Gly Pro 595 600 605 Leu < 210 > 101 < 211 > 609 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : huRIPK 1 ( 1 - 555 ) . RR . DM ; TH 1023 without epitope tag < 400 > 101 Met Gln Pro Asp Met Ser Leu Asn Val Ile Lys Met Lys Ser Ser Asp 1 5 10 15 Phe Leu Glu Ser Ala Glu Leu Asp Ser Gly Gly Phe Gly Lys Val Ser 20 25 30 Leu Cys Phe His Arg Thr Gln Gly Leu Met Ile Met Lys Thr Val Tyr 35 40 45 Lys Gly Pro Asn Cys Ile Glu His Asn Glu Ala Leu Leu Glu Glu Ala 50 55 60 Lys Met Met Asn Arg Leu Arg His Ser Arg Val Val Lys Leu Leu Gly 65 70 75 80 Val Ile Ile Glu Glu Gly Lys Tyr Ser Leu Val Met Glu Tyr Met Glu 85 90 95 Lys Gly Asn Leu Met His Val Leu Lys Ala Glu Met Ser Thr Pro Leu 100 105 110 Ser Val Lys Gly Arg Ile Ile Leu Glu Ile Ile Glu Gly Met Cys Tyr 115 120 125 Leu His Gly Lys Gly Val Ile His Lys Asp Leu Lys Pro Glu Asn Ile 130 135 140 Leu Val Asp Asn Asp Phe His Ile Lys Ile Ala Asp Leu Gly Leu Ala 145 150 155 160 Ser Phe Lys Met Trp Ser Lys Leu Asn Asn Glu Glu His Asn Glu Leu 165 170 175 Arg Glu Val Asp Gly Thr Ala Lys Lys Asn Gly Gly Thr Leu Tyr Tyr 180 185 190 Met Ala Pro Glu His Leu Asn Asp Val Asn Ala Lys Pro Thr Glu Lys 195 200 205 Ser Asp Val Tyr Ser Phe Ala Val Val Leu Trp Ala Ile Phe Ala Asn 210 215 220 Lys Glu Pro Tyr Glu Asn Ala Ile Cys Glu Gln Gln Leu Ile Met Cys 225 230 235 240 Ile Lys Ser Gly Asn Arg Pro Asp Val Asp Asp Ile Thr Glu Tyr Cys 245 250 255 Pro Arg Glu Ile Ile Ser Leu Met Lys Leu Cys Trp Glu Ala Asn Pro 260 265 270 Glu Ala Arg Pro Thr Phe Pro Gly Ile Glu Glu Lys Phe Arg Pro Phe 275 280 285 Tyr Leu Ser Gln Leu Glu Glu Ser Val Glu Glu Asp Val Lys Ser Leu 290 295 300 Lys Lys Glu Tyr Ser Asn Glu Asn Ala Val Val Lys Arg Met Gln Ser 305 310 315 320 Leu Gln Leu Asp Cys Val Ala Val Pro Ser Ser Arg Ser Asn Ser Ala 325 330 335 Thr Glu Gln Pro Gly Ser Leu His Ser Ser Gln Gly Leu Gly Met Gly 340 345 350 Pro Val Glu Glu Ser Trp Phe Ala Pro Ser Leu Glu His Pro Gln Glu 355 360 365 Glu Asn Glu Pro Ser Leu Gln Ser Lys Leu Gln Asp Glu Ala Asn Tyr 370 375 380 His Leu Tyr Gly Ser Arg Met Asp Arg Gln Thr Lys Gln Gln Pro Arg 385 390 395 400 Gln Asn Val Ala Tyr Asn Arg Glu Glu Glu Arg Arg Arg Arg Val Ser 405 410 415 His Asp Pro Phe Ala Gln Gln Arg Pro Tyr Glu Asn Phe Gln Asn Thr 420 425 430 Glu Gly Lys Gly Thr Ala Tyr Ser Ser Ala Ala Ser His Gly Asn Ala 435 440 445 Val His Gln Pro Ser Gly Leu Thr Ser Gln Pro Gln Val Leu Tyr Gln 450 455 460 Asn Asn Gly Leu Tyr Ser Ser His Gly Phe Gly Thr Arg Pro Leu Asp 465 470 475 480 Pro Gly Thr Ala Gly Pro Arg Val Trp Tyr Arg Pro Ile Pro Ser His 485 490 495 Met Pro Ser Leu His Asn Ile Pro Val Pro Glu Thr Asn Tyr Leu Gly 500 505 510 Asn Thr Pro Thr Met Pro Phe Ser Ser Leu Pro Pro Thr Asp Glu Ser 515 520 525 Ile Lys Tyr Thr Ile Tyr Asn Ser Thr Gly Ile Gln Ile Gly Ala Tyr 530 535 540 Asn Tyr Met Glu Ile Gly Gly Thr Ser Ser Ser Gly Ser Asp Gly Ser 545 550 555 560 Gly Ser Gly Ser Gly Ser Leu Glu Ile Arg Ala Ala Phe Leu Glu Lys 565 570 575 Glu Asn Thr Ala Leu Arg Thr Arg Ala Ala Glu Leu Arg Lys Arg Val 580 585 590 Gly Arg Cys Arg Asn Ile Val Ser Lys Tyr Glu Thr Arg Tyr Gly Pro 595 600 605 Leu < 210 > 102 < 211 > 585 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : msRIPK 1 ( 1 - 555 ) . IZ . TM ; TH 1024 without epitope tag < 400 > 102 Met Gln Pro Asp Met Ser Leu Asp Asn Ile Lys Met Ala Ser Ser Asp 1 5 10 15 Leu Leu Glu Lys Thr Asp Leu Asp Ser Gly Gly Phe Gly Lys Val Ser 20 25 30 Leu Cys Tyr His Arg Ser His Gly Phe Val Ile Leu Lys Lys Val Tyr 35 40 45 Thr Gly Pro Asn Arg Ala Glu Tyr Asn Glu Val Leu Leu Glu Glu Gly 50 55 60 Lys Met Met His Arg Leu Arg His Ser Arg Val Val Lys Leu Leu Gly 65 70 75 80 Ile Ile Ile Glu Glu Gly Asn Tyr Ser Leu Val Met Glu Tyr Met Glu 85 90 95 Lys Gly Asn Leu Met His Val Leu Lys Thr Gln Ile Asp Val Pro Leu 100 105 110 Ser Leu Lys Gly Arg Ile Ile Val Glu Ala Ile Glu Gly Met Cys Tyr 115 120 125 Leu His Asp Lys Gly Val Ile His Lys Asp Leu Lys Pro Glu Asn Ile 130 135 140 Leu Val Asp Arg Asp Phe His Ile Lys Ile Ala Asp Leu Gly Val Ala 145 150 155 160 Ser Phe Lys Thr Trp Ser Lys Leu Thr Lys Glu Lys Asp Asn Lys Gln 165 170 175 Lys Glu Val Ser Ser Thr Thr Lys Lys Asn Asn Gly Gly Thr Leu Tyr 180 185 190 Tyr Met Ala Pro Glu His Leu Asn Asp Ile Asn Ala Lys Pro Thr Glu 195 200 205 Lys Ser Asp Val Tyr Ser Phe Gly Ile Val Leu Trp Ala Ile Phe Ala 210 215 220 Lys Lys Glu Pro Tyr Glu Asn Val Ile Cys Thr Glu Gln Phe Val Ile 225 230 235 240 Cys Ile Lys Ser Gly Asn Arg Pro Asn Val Glu Glu Ile Leu Glu Tyr 245 250 255 Cys Pro Arg Glu Ile Ile Ser Leu Met Glu Arg Cys Trp Gln Ala Ile 260 265 270 Pro Glu Asp Arg Pro Thr Phe Leu Gly Ile Glu Glu Glu Phe Arg Pro 275 280 285 Phe Tyr Leu Ser His Phe Glu Glu Tyr Val Glu Glu Asp Val Ala Ser 290 295 300 Leu Lys Lys Glu Tyr Pro Asp Gln Ser Pro Val Leu Gln Arg Met Phe 305 310 315 320 Ser Leu Gln His Asp Cys Val Pro Leu Pro Pro Ser Arg Ser Asn Ser 325 330 335 Glu Gln Pro Gly Ser Leu His Ser Ser Gln Gly Leu Gln Met Gly Pro 340 345 350 Val Glu Glu Ser Trp Phe Ser Ser Ser Pro Glu Tyr Pro Gln Asp Glu 355 360 365 Asn Asp Arg Ser Val Gln Ala Lys Leu Gln Glu Glu Ala Ser Tyr His 370 375 380 Ala Phe Gly Ile Phe Ala Glu Lys Gln Thr Lys Pro Gln Pro Arg Gln 385 390 395 400 Asn Glu Ala Tyr Asn Arg Glu Glu Glu Arg Lys Arg Arg Val Ser His 405 410 415 Asp Pro Phe Ala Gln Gln Arg Ala Arg Glu Asn Ile Lys Ser Ala Gly 420 425 430 Ala Arg Gly His Ser Asp Pro Ser Thr Thr Ser Arg Gly Ile Ala Val 435 440 445 Gln Gln Leu Ser Trp Pro Ala Thr Gln Thr Val Trp Asn Asn Gly Leu 450 455 460 Tyr Asn Gln His Gly Phe Gly Thr Thr Gly Thr Gly Val Trp Tyr Pro 465 470 475 480 Pro Asn Leu Ser Gln Met Tyr Ser Thr Tyr Lys Thr Pro Val Pro Glu 485 490 495 Thr Asn Ile Pro Gly Ser Thr Pro Thr Met Pro Tyr Phe Ser Gly Pro 500 505 510 Val Ala Asp Asp Leu Ile Lys Tyr Thr Ile Phe Asn Ser Ser Gly Ile 515 520 525 Gln Ile Gly Asn His Asn Tyr Met Asp Val Gly Leu Asn Ser Gln Pro 530 535 540 Pro Asn Asn Thr Cys Lys Glu Glu Ser Thr Ser Gly Gly Ile Lys Lys 545 550 555 560 Glu Ile Glu Ala Ile Lys Lys Glu Gln Glu Ala Ile Lys Lys Lys Ile 565 570 575 Glu Ala Ile Glu Lys Glu Ile Glu Ala 580 585 < 210 > 103 < 211 > 609 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : msRIPK 1 ( 1 - 555 ) . EE . DM ; TH 1025 without epitope tag < 400 > 103 Met Gln Pro Asp Met Ser Leu Asp Asn Ile Lys Met Ala Ser Ser Asp 1 5 10 15 Leu Leu Glu Lys Thr Asp Leu Asp Ser Gly Gly Phe Gly Lys Val Ser 20 25 30 Leu Cys Tyr His Arg Ser His Gly Phe Val Ile Leu Lys Lys Val Tyr 35 40 45 Thr Gly Pro Asn Arg Ala Glu Tyr Asn Glu Val Leu Leu Glu Glu Gly 50 55 60 Lys Met Met His Arg Leu Arg His Ser Arg Val Val Lys Leu Leu Gly 65 70 75 80 Ile Ile Ile Glu Glu Gly Asn Tyr Ser Leu Val Met Glu Tyr Met Glu 85 90 95 Lys Gly Asn Leu Met His Val Leu Lys Thr Gln Ile Asp Val Pro Leu 100 105 110 Ser Leu Lys Gly Arg Ile Ile Val Glu Ala Ile Glu Gly Met Cys Tyr 115 120 125 Leu His Asp Lys Gly Val Ile His Lys Asp Leu Lys Pro Glu Asn Ile 130 135 140 Leu Val Asp Arg Asp Phe His Ile Lys Ile Ala Asp Leu Gly Val Ala 145 150 155 160 Ser Phe Lys Thr Trp Ser Lys Leu Thr Lys Glu Lys Asp Asn Lys Gln 165 170 175 Lys Glu Val Ser Ser Thr Thr Lys Lys Asn Asn Gly Gly Thr Leu Tyr 180 185 190 Tyr Met Ala Pro Glu His Leu Asn Asp Ile Asn Ala Lys Pro Thr Glu 195 200 205 Lys Ser Asp Val Tyr Ser Phe Gly Ile Val Leu Trp Ala Ile Phe Ala 210 215 220 Lys Lys Glu Pro Tyr Glu Asn Val Ile Cys Thr Glu Gln Phe Val Ile 225 230 235 240 Cys Ile Lys Ser Gly Asn Arg Pro Asn Val Glu Glu Ile Leu Glu Tyr 245 250 255 Cys Pro Arg Glu Ile Ile Ser Leu Met Glu Arg Cys Trp Gln Ala Ile 260 265 270 Pro Glu Asp Arg Pro Thr Phe Leu Gly Ile Glu Glu Glu Phe Arg Pro 275 280 285 Phe Tyr Leu Ser His Phe Glu Glu Tyr Val Glu Glu Asp Val Ala Ser 290 295 300 Leu Lys Lys Glu Tyr Pro Asp Gln Ser Pro Val Leu Gln Arg Met Phe 305 310 315 320 Ser Leu Gln His Asp Cys Val Pro Leu Pro Pro Ser Arg Ser Asn Ser 325 330 335 Glu Gln Pro Gly Ser Leu His Ser Ser Gln Gly Leu Gln Met Gly Pro 340 345 350 Val Glu Glu Ser Trp Phe Ser Ser Ser Pro Glu Tyr Pro Gln Asp Glu 355 360 365 Asn Asp Arg Ser Val Gln Ala Lys Leu Gln Glu Glu Ala Ser Tyr His 370 375 380 Ala Phe Gly Ile Phe Ala Glu Lys Gln Thr Lys Pro Gln Pro Arg Gln 385 390 395 400 Asn Glu Ala Tyr Asn Arg Glu Glu Glu Arg Lys Arg Arg Val Ser His 405 410 415 Asp Pro Phe Ala Gln Gln Arg Ala Arg Glu Asn Ile Lys Ser Ala Gly 420 425 430 Ala Arg Gly His Ser Asp Pro Ser Thr Thr Ser Arg Gly Ile Ala Val 435 440 445 Gln Gln Leu Ser Trp Pro Ala Thr Gln Thr Val Trp Asn Asn Gly Leu 450 455 460 Tyr Asn Gln His Gly Phe Gly Thr Thr Gly Thr Gly Val Trp Tyr Pro 465 470 475 480 Pro Asn Leu Ser Gln Met Tyr Ser Thr Tyr Lys Thr Pro Val Pro Glu 485 490 495 Thr Asn Ile Pro Gly Ser Thr Pro Thr Met Pro Tyr Phe Ser Gly Pro 500 505 510 Val Ala Asp Asp Leu Ile Lys Tyr Thr Ile Phe Asn Ser Ser Gly Ile 515 520 525 Gln Ile Gly Asn His Asn Tyr Met Asp Val Gly Leu Asn Ser Gln Pro 530 535 540 Pro Asn Asn Thr Cys Lys Glu Glu Ser Thr Ser Gly Ser Asp Gly Ser 545 550 555 560 Gly Ser Gly Ser Gly Ser Ile Thr Ile Arg Ala Ala Phe Leu Glu Lys 565 570 575 Glu Asn Thr Ala Leu Arg Thr Glu Ile Ala Glu Leu Glu Lys Glu Val 580 585 590 Gly Arg Cys Glu Asn Ile Val Ser Lys Tyr Glu Thr Arg Tyr Gly Pro 595 600 605 Leu < 210 > 104 < 211 > 609 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : msRIPK 1 ( 1 - 555 ) . RR . DM ; TH 1026 without epitope tag < 400 > 104 Met Gln Pro Asp Met Ser Leu Asp Asn Ile Lys Met Ala Ser Ser Asp 1 5 10 15 Leu Leu Glu Lys Thr Asp Leu Asp Ser Gly Gly Phe Gly Lys Val Ser 20 25 30 Leu Cys Tyr His Arg Ser His Gly Phe Val Ile Leu Lys Lys Val Tyr 35 40 45 Thr Gly Pro Asn Arg Ala Glu Tyr Asn Glu Val Leu Leu Glu Glu Gly 50 55 60 Lys Met Met His Arg Leu Arg His Ser Arg Val Val Lys Leu Leu Gly 65 70 75 80 Ile Ile Ile Glu Glu Gly Asn Tyr Ser Leu Val Met Glu Tyr Met Glu 85 90 95 Lys Gly Asn Leu Met His Val Leu Lys Thr Gln Ile Asp Val Pro Leu 100 105 110 Ser Leu Lys Gly Arg Ile Ile Val Glu Ala Ile Glu Gly Met Cys Tyr 115 120 125 Leu His Asp Lys Gly Val Ile His Lys Asp Leu Lys Pro Glu Asn Ile 130 135 140 Leu Val Asp Arg Asp Phe His Ile Lys Ile Ala Asp Leu Gly Val Ala 145 150 155 160 Ser Phe Lys Thr Trp Ser Lys Leu Thr Lys Glu Lys Asp Asn Lys Gln 165 170 175 Lys Glu Val Ser Ser Thr Thr Lys Lys Asn Asn Gly Gly Thr Leu Tyr 180 185 190 Tyr Met Ala Pro Glu His Leu Asn Asp Ile Asn Ala Lys Pro Thr Glu 195 200 205 Lys Ser Asp Val Tyr Ser Phe Gly Ile Val Leu Trp Ala Ile Phe Ala 210 215 220 Lys Lys Glu Pro Tyr Glu Asn Val Ile Cys Thr Glu Gln Phe Val Ile 225 230 235 240 Cys Ile Lys Ser Gly Asn Arg Pro Asn Val Glu Glu Ile Leu Glu Tyr 245 250 255 Cys Pro Arg Glu Ile Ile Ser Leu Met Glu Arg Cys Trp Gln Ala Ile 260 265 270 Pro Glu Asp Arg Pro Thr Phe Leu Gly Ile Glu Glu Glu Phe Arg Pro 275 280 285 Phe Tyr Leu Ser His Phe Glu Glu Tyr Val Glu Glu Asp Val Ala Ser 290 295 300 Leu Lys Lys Glu Tyr Pro Asp Gln Ser Pro Val Leu Gln Arg Met Phe 305 310 315 320 Ser Leu Gln His Asp Cys Val Pro Leu Pro Pro Ser Arg Ser Asn Ser 325 330 335 Glu Gln Pro Gly Ser Leu His Ser Ser Gln Gly Leu Gln Met Gly Pro 340 345 350 Val Glu Glu Ser Trp Phe Ser Ser Ser Pro Glu Tyr Pro Gln Asp Glu 355 360 365 Asn Asp Arg Ser Val Gln Ala Lys Leu Gln Glu Glu Ala Ser Tyr His 370 375 380 Ala Phe Gly Ile Phe Ala Glu Lys Gln Thr Lys Pro Gln Pro Arg Gln 385 390 395 400 Asn Glu Ala Tyr Asn Arg Glu Glu Glu Arg Lys Arg Arg Val Ser His 405 410 415 Asp Pro Phe Ala Gln Gln Arg Ala Arg Glu Asn Ile Lys Ser Ala Gly 420 425 430 Ala Arg Gly His Ser Asp Pro Ser Thr Thr Ser Arg Gly Ile Ala Val 435 440 445 Gln Gln Leu Ser Trp Pro Ala Thr Gln Thr Val Trp Asn Asn Gly Leu 450 455 460 Tyr Asn Gln His Gly Phe Gly Thr Thr Gly Thr Gly Val Trp Tyr Pro 465 470 475 480 Pro Asn Leu Ser Gln Met Tyr Ser Thr Tyr Lys Thr Pro Val Pro Glu 485 490 495 Thr Asn Ile Pro Gly Ser Thr Pro Thr Met Pro Tyr Phe Ser Gly Pro 500 505 510 Val Ala Asp Asp Leu Ile Lys Tyr Thr Ile Phe Asn Ser Ser Gly Ile 515 520 525 Gln Ile Gly Asn His Asn Tyr Met Asp Val Gly Leu Asn Ser Gln Pro 530 535 540 Pro Asn Asn Thr Cys Lys Glu Glu Ser Thr Ser Gly Ser Asp Gly Ser 545 550 555 560 Gly Ser Gly Ser Gly Ser Leu Glu Ile Arg Ala Ala Phe Leu Glu Lys 565 570 575 Glu Asn Thr Ala Leu Arg Thr Arg Ala Ala Glu Leu Arg Lys Arg Val 580 585 590 Gly Arg Cys Arg Asn Ile Val Ser Lys Tyr Glu Thr Arg Tyr Gly Pro 595 600 605 Leu < 210 > 105 < 211 > 378 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 378 ) < 223 > human TAK 1 - TAB 1 ; P 4031 without epitope tag < 400 > 105 Met Ser Thr Ala Ser Ala Ala Ser Ser Ser Ser Ser Ser Ser Ala Gly 1 5 10 15 Glu Met Ile Glu Ala Pro Ser Gln Val Leu Asn Phe Glu Glu Ile Asp 20 25 30 Tyr Lys Glu Ile Glu Val Glu Glu Val Val Gly Arg Gly Ala Phe Gly 35 40 45 Val Val Cys Lys Ala Lys Trp Arg Ala Lys Asp Val Ala Ile Lys Gln 50 55 60 Ile Glu Ser Glu Ser Glu Arg Lys Ala Phe Ile Val Glu Leu Arg Gln 65 70 75 80 Leu Ser Arg Val Asn His Pro Asn Ile Val Lys Leu Tyr Gly Ala Cys 85 90 95 Leu Asn Pro Val Cys Leu Val Met Glu Tyr Ala Glu Gly Gly Ser Leu 100 105 110 Tyr Asn Val Leu His Gly Ala Glu Pro Leu Pro Tyr Tyr Thr Ala Ala 115 120 125 His Ala Met Ser Trp Cys Leu Gln Cys Ser Gln Gly Val Ala Tyr Leu 130 135 140 His Ser Met Gln Pro Lys Ala Leu Ile His Arg Asp Leu Lys Pro Pro 145 150 155 160 Asn Leu Leu Leu Val Ala Gly Gly Thr Val Leu Lys Ile Cys Asp Phe 165 170 175 Gly Thr Ala Cys Asp Ile Gln Thr His Met Thr Asn Asn Lys Gly Ser 180 185 190 Ala Ala Trp Met Ala Pro Glu Val Phe Glu Gly Ser Asn Tyr Ser Glu 195 200 205 Lys Cys Asp Val Phe Ser Trp Gly Ile Ile Leu Trp Glu Val Ile Thr 210 215 220 Arg Arg Lys Pro Phe Asp Glu Ile Gly Gly Pro Ala Phe Arg Ile Met 225 230 235 240 Trp Ala Val His Asn Gly Thr Arg Pro Pro Leu Ile Lys Asn Leu Pro 245 250 255 Lys Pro Ile Glu Ser Leu Met Thr Arg Cys Trp Ser Lys Asp Pro Ser 260 265 270 Gln Arg Pro Ser Met Glu Glu Ile Val Lys Ile Met Thr His Leu Met 275 280 285 Arg Tyr Phe Pro Gly Ala Asp Glu Pro Leu Gln Tyr Pro Cys Gln Glu 290 295 300 Phe Gly Gly Gly Gly Gly Gln Ser Pro Thr Leu Thr Leu Gln Ser Thr 305 310 315 320 Asn Thr His Thr Gln Ser Ser Ser Ser Ser Ser Asp Gly Gly Leu Phe 325 330 335 Arg Ser Arg Pro Ala His Ser Leu Pro Pro Gly Glu Asp Gly Arg Val 340 345 350 Glu Pro Tyr Val Asp Phe Ala Glu Phe Tyr Arg Leu Trp Ser Val Asp 355 360 365 His Gly Glu Gln Ser Val Val Thr Ala Pro 370 375 < 210 > 106 < 211 > 239 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Diablo . 1 ; without epitope tag < 400 > 106 Met Ala Ala Leu Lys Ser Trp Leu Ser Arg Ser Val Thr Ser Phe Phe 1 5 10 15 Arg Tyr Arg Gln Cys Leu Cys Val Pro Val Val Ala Asn Phe Lys Lys 20 25 30 Arg Cys Phe Ser Glu Leu Ile Arg Pro Trp His Lys Thr Val Thr Ile 35 40 45 Gly Phe Gly Val Thr Leu Cys Ala Val Pro Ile Ala Gln Lys Ser Glu 50 55 60 Pro His Ser Leu Ser Ser Glu Ala Leu Met Arg Arg Ala Val Ser Leu 65 70 75 80 Val Thr Asp Ser Thr Ser Thr Phe Leu Ser Gln Thr Thr Tyr Ala Leu 85 90 95 Ile Glu Ala Ile Thr Glu Tyr Thr Lys Ala Val Tyr Thr Leu Thr Ser 100 105 110 Leu Tyr Arg Gln Tyr Thr Ser Leu Leu Gly Lys Met Asn Ser Glu Glu 115 120 125 Glu Asp Glu Val Trp Gln Val Ile Ile Gly Ala Arg Ala Glu Met Thr 130 135 140 Ser Lys His Gln Glu Tyr Leu Lys Leu Glu Thr Thr Trp Met Thr Ala 145 150 155 160 Val Gly Leu Ser Glu Met Ala Ala Glu Ala Ala Tyr Gln Thr Gly Ala 165 170 175 Asp Gln Ala Ser Ile Thr Ala Arg Asn His Ile Gln Leu Val Lys Leu 180 185 190 Gln Val Glu Glu Val His Gln Leu Ser Arg Lys Ala Glu Thr Lys Leu 195 200 205 Ala Glu Ala Gln Ile Glu Glu Leu Arg Gln Lys Thr Gln Glu Glu Gly 210 215 220 Glu Glu Arg Ala Glu Ser Glu Gln Glu Ala Tyr Leu Arg Glu Asp 225 230 235 < 210 > 107 < 211 > 239 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Diablo . 1 ( S 126 L ) ; without epitope tag < 400 > 107 Met Ala Ala Leu Lys Ser Trp Leu Ser Arg Ser Val Thr Ser Phe Phe 1 5 10 15 Arg Tyr Arg Gln Cys Leu Cys Val Pro Val Val Ala Asn Phe Lys Lys 20 25 30 Arg Cys Phe Ser Glu Leu Ile Arg Pro Trp His Lys Thr Val Thr Ile 35 40 45 Gly Phe Gly Val Thr Leu Cys Ala Val Pro Ile Ala Gln Lys Ser Glu 50 55 60 Pro His Ser Leu Ser Ser Glu Ala Leu Met Arg Arg Ala Val Ser Leu 65 70 75 80 Val Thr Asp Ser Thr Ser Thr Phe Leu Ser Gln Thr Thr Tyr Ala Leu 85 90 95 Ile Glu Ala Ile Thr Glu Tyr Thr Lys Ala Val Tyr Thr Leu Thr Ser 100 105 110 Leu Tyr Arg Gln Tyr Thr Ser Leu Leu Gly Lys Met Asn Leu Glu Glu 115 120 125 Glu Asp Glu Val Trp Gln Val Ile Ile Gly Ala Arg Ala Glu Met Thr 130 135 140 Ser Lys His Gln Glu Tyr Leu Lys Leu Glu Thr Thr Trp Met Thr Ala 145 150 155 160 Val Gly Leu Ser Glu Met Ala Ala Glu Ala Ala Tyr Gln Thr Gly Ala 165 170 175 Asp Gln Ala Ser Ile Thr Ala Arg Asn His Ile Gln Leu Val Lys Leu 180 185 190 Gln Val Glu Glu Val His Gln Leu Ser Arg Lys Ala Glu Thr Lys Leu 195 200 205 Ala Glu Ala Gln Ile Glu Glu Leu Arg Gln Lys Thr Gln Glu Glu Gly 210 215 220 Glu Glu Arg Ala Glu Ser Glu Gln Glu Ala Tyr Leu Arg Glu Asp 225 230 235 < 210 > 108 < 211 > 185 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Diablo . 1 ( 56 - 239 ) ; without epitope tag < 400 > 108 Met Ala Val Pro Ile Ala Gln Lys Ser Glu Pro His Ser Leu Ser Ser 1 5 10 15 Glu Ala Leu Met Arg Arg Ala Val Ser Leu Val Thr Asp Ser Thr Ser 20 25 30 Thr Phe Leu Ser Gln Thr Thr Tyr Ala Leu Ile Glu Ala Ile Thr Glu 35 40 45 Tyr Thr Lys Ala Val Tyr Thr Leu Thr Ser Leu Tyr Arg Gln Tyr Thr 50 55 60 Ser Leu Leu Gly Lys Met Asn Ser Glu Glu Glu Asp Glu Val Trp Gln 65 70 75 80 Val Ile Ile Gly Ala Arg Ala Glu Met Thr Ser Lys His Gln Glu Tyr 85 90 95 Leu Lys Leu Glu Thr Thr Trp Met Thr Ala Val Gly Leu Ser Glu Met 100 105 110 Ala Ala Glu Ala Ala Tyr Gln Thr Gly Ala Asp Gln Ala Ser Ile Thr 115 120 125 Ala Arg Asn His Ile Gln Leu Val Lys Leu Gln Val Glu Glu Val His 130 135 140 Gln Leu Ser Arg Lys Ala Glu Thr Lys Leu Ala Glu Ala Gln Ile Glu 145 150 155 160 Glu Leu Arg Gln Lys Thr Gln Glu Glu Gly Glu Glu Arg Ala Glu Ser 165 170 175 Glu Gln Glu Ala Tyr Leu Arg Glu Asp 180 185 < 210 > 109 < 211 > 185 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Diablo . 1 ( 56 - 239 / S 126 L ) ; without epitope tag < 400 > 109 Met Ala Val Pro Ile Ala Gln Lys Ser Glu Pro His Ser Leu Ser Ser 1 5 10 15 Glu Ala Leu Met Arg Arg Ala Val Ser Leu Val Thr Asp Ser Thr Ser 20 25 30 Thr Phe Leu Ser Gln Thr Thr Tyr Ala Leu Ile Glu Ala Ile Thr Glu 35 40 45 Tyr Thr Lys Ala Val Tyr Thr Leu Thr Ser Leu Tyr Arg Gln Tyr Thr 50 55 60 Ser Leu Leu Gly Lys Met Asn Leu Glu Glu Glu Asp Glu Val Trp Gln 65 70 75 80 Val Ile Ile Gly Ala Arg Ala Glu Met Thr Ser Lys His Gln Glu Tyr 85 90 95 Leu Lys Leu Glu Thr Thr Trp Met Thr Ala Val Gly Leu Ser Glu Met 100 105 110 Ala Ala Glu Ala Ala Tyr Gln Thr Gly Ala Asp Gln Ala Ser Ile Thr 115 120 125 Ala Arg Asn His Ile Gln Leu Val Lys Leu Gln Val Glu Glu Val His 130 135 140 Gln Leu Ser Arg Lys Ala Glu Thr Lys Leu Ala Glu Ala Gln Ile Glu 145 150 155 160 Glu Leu Arg Gln Lys Thr Gln Glu Glu Gly Glu Glu Arg Ala Glu Ser 165 170 175 Glu Gln Glu Ala Tyr Leu Arg Glu Asp 180 185 < 210 > 110 < 211 > 195 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Diablo . 3 ; TH 2003 without epitope tag < 400 > 110 Met Ala Ala Leu Lys Ser Trp Leu Ser Arg Ser Val Thr Ser Phe Phe 1 5 10 15 Arg Tyr Arg Gln Cys Leu Cys Val Pro Val Val Ala Asn Phe Lys Lys 20 25 30 Arg Cys Phe Ser Glu Leu Ile Arg Pro Trp His Lys Thr Val Thr Ile 35 40 45 Gly Phe Gly Val Thr Leu Cys Ala Val Pro Ile Ala Gln Ala Val Tyr 50 55 60 Thr Leu Thr Ser Leu Tyr Arg Gln Tyr Thr Ser Leu Leu Gly Lys Met 65 70 75 80 Asn Ser Glu Glu Glu Asp Glu Val Trp Gln Val Ile Ile Gly Ala Arg 85 90 95 Ala Glu Met Thr Ser Lys His Gln Glu Tyr Leu Lys Leu Glu Thr Thr 100 105 110 Trp Met Thr Ala Val Gly Leu Ser Glu Met Ala Ala Glu Ala Ala Tyr 115 120 125 Gln Thr Gly Ala Asp Gln Ala Ser Ile Thr Ala Arg Asn His Ile Gln 130 135 140 Leu Val Lys Leu Gln Val Glu Glu Val His Gln Leu Ser Arg Lys Ala 145 150 155 160 Glu Thr Lys Leu Ala Glu Ala Gln Ile Glu Glu Leu Arg Gln Lys Thr 165 170 175 Gln Glu Glu Gly Glu Glu Arg Ala Glu Ser Glu Gln Glu Ala Tyr Leu 180 185 190 Arg Glu Asp 195 < 210 > 111 < 211 > 195 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Diablo . 3 ( S 82 L ) ; TH 2001 without epitope tag < 400 > 111 Met Ala Ala Leu Lys Ser Trp Leu Ser Arg Ser Val Thr Ser Phe Phe 1 5 10 15 Arg Tyr Arg Gln Cys Leu Cys Val Pro Val Val Ala Asn Phe Lys Lys 20 25 30 Arg Cys Phe Ser Glu Leu Ile Arg Pro Trp His Lys Thr Val Thr Ile 35 40 45 Gly Phe Gly Val Thr Leu Cys Ala Val Pro Ile Ala Gln Ala Val Tyr 50 55 60 Thr Leu Thr Ser Leu Tyr Arg Gln Tyr Thr Ser Leu Leu Gly Lys Met 65 70 75 80 Asn Leu Glu Glu Glu Asp Glu Val Trp Gln Val Ile Ile Gly Ala Arg 85 90 95 Ala Glu Met Thr Ser Lys His Gln Glu Tyr Leu Lys Leu Glu Thr Thr 100 105 110 Trp Met Thr Ala Val Gly Leu Ser Glu Met Ala Ala Glu Ala Ala Tyr 115 120 125 Gln Thr Gly Ala Asp Gln Ala Ser Ile Thr Ala Arg Asn His Ile Gln 130 135 140 Leu Val Lys Leu Gln Val Glu Glu Val His Gln Leu Ser Arg Lys Ala 145 150 155 160 Glu Thr Lys Leu Ala Glu Ala Gln Ile Glu Glu Leu Arg Gln Lys Thr 165 170 175 Gln Glu Glu Gly Glu Glu Arg Ala Glu Ser Glu Gln Glu Ala Tyr Leu 180 185 190 Arg Glu Asp 195 < 210 > 112 < 211 > 141 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Diablo . 3 ( 56 - 195 ) ; TH 2002 without epitope tag < 400 > 112 Met Ala Val Pro Ile Ala Gln Ala Val Tyr Thr Leu Thr Ser Leu Tyr 1 5 10 15 Arg Gln Tyr Thr Ser Leu Leu Gly Lys Met Asn Ser Glu Glu Glu Asp 20 25 30 Glu Val Trp Gln Val Ile Ile Gly Ala Arg Ala Glu Met Thr Ser Lys 35 40 45 His Gln Glu Tyr Leu Lys Leu Glu Thr Thr Trp Met Thr Ala Val Gly 50 55 60 Leu Ser Glu Met Ala Ala Glu Ala Ala Tyr Gln Thr Gly Ala Asp Gln 65 70 75 80 Ala Ser Ile Thr Ala Arg Asn His Ile Gln Leu Val Lys Leu Gln Val 85 90 95 Glu Glu Val His Gln Leu Ser Arg Lys Ala Glu Thr Lys Leu Ala Glu 100 105 110 Ala Gln Ile Glu Glu Leu Arg Gln Lys Thr Gln Glu Glu Gly Glu Glu 115 120 125 Arg Ala Glu Ser Glu Gln Glu Ala Tyr Leu Arg Glu Asp 130 135 140 < 210 > 113 < 211 > 141 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Diablo . 3 ( 56 - 195 / S 82 L ) ; without epitope tag < 400 > 113 Met Ala Val Pro Ile Ala Gln Ala Val Tyr Thr Leu Thr Ser Leu Tyr 1 5 10 15 Arg Gln Tyr Thr Ser Leu Leu Gly Lys Met Asn Leu Glu Glu Glu Asp 20 25 30 Glu Val Trp Gln Val Ile Ile Gly Ala Arg Ala Glu Met Thr Ser Lys 35 40 45 His Gln Glu Tyr Leu Lys Leu Glu Thr Thr Trp Met Thr Ala Val Gly 50 55 60 Leu Ser Glu Met Ala Ala Glu Ala Ala Tyr Gln Thr Gly Ala Asp Gln 65 70 75 80 Ala Ser Ile Thr Ala Arg Asn His Ile Gln Leu Val Lys Leu Gln Val 85 90 95 Glu Glu Val His Gln Leu Ser Arg Lys Ala Glu Thr Lys Leu Ala Glu 100 105 110 Ala Gln Ile Glu Glu Leu Arg Gln Lys Thr Gln Glu Glu Gly Glu Glu 115 120 125 Arg Ala Glu Ser Glu Gln Glu Ala Tyr Leu Arg Glu Asp 130 135 140 < 210 > 114 < 211 > 659 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Btk ( E 41 K ) ; P 4029 without epitope tag < 400 > 114 Met Ala Ala Val Ile Leu Glu Ser Ile Phe Leu Lys Arg Ser Gln Gln 1 5 10 15 Lys Lys Lys Thr Ser Pro Leu Asn Phe Lys Lys Arg Leu Phe Leu Leu 20 25 30 Thr Val His Lys Leu Ser Tyr Tyr Lys Tyr Asp Phe Glu Arg Gly Arg 35 40 45 Arg Gly Ser Lys Lys Gly Ser Ile Asp Val Glu Lys Ile Thr Cys Val 50 55 60 Glu Thr Val Val Pro Glu Lys Asn Pro Pro Pro Glu Arg Gln Ile Pro 65 70 75 80 Arg Arg Gly Glu Glu Ser Ser Glu Met Glu Gln Ile Ser Ile Ile Glu 85 90 95 Arg Phe Pro Tyr Pro Phe Gln Val Val Tyr Asp Glu Gly Pro Leu Tyr 100 105 110 Val Phe Ser Pro Thr Glu Glu Leu Arg Lys Arg Trp Ile His Gln Leu 115 120 125 Lys Asn Val Ile Arg Tyr Asn Ser Asp Leu Val Gln Lys Tyr His Pro 130 135 140 Cys Phe Trp Ile Asp Gly Gln Tyr Leu Cys Cys Ser Gln Thr Ala Lys 145 150 155 160 Asn Ala Met Gly Cys Gln Ile Leu Glu Asn Arg Asn Gly Ser Leu Lys 165 170 175 Pro Gly Ser Ser His Arg Lys Thr Lys Lys Pro Leu Pro Pro Thr Pro 180 185 190 Glu Glu Asp Gln Ile Leu Lys Lys Pro Leu Pro Pro Glu Pro Ala Ala 195 200 205 Ala Pro Val Ser Thr Ser Glu Leu Lys Lys Val Val Ala Leu Tyr Asp 210 215 220 Tyr Met Pro Met Asn Ala Asn Asp Leu Gln Leu Arg Lys Gly Asp Glu 225 230 235 240 Tyr Phe Ile Leu Glu Glu Ser Asn Leu Pro Trp Trp Arg Ala Arg Asp 245 250 255 Lys Asn Gly Gln Glu Gly Tyr Ile Pro Ser Asn Tyr Val Thr Glu Ala 260 265 270 Glu Asp Ser Ile Glu Met Tyr Glu Trp Tyr Ser Lys His Met Thr Arg 275 280 285 Ser Gln Ala Glu Gln Leu Leu Lys Gln Glu Gly Lys Glu Gly Gly Phe 290 295 300 Ile Val Arg Asp Ser Ser Lys Ala Gly Lys Tyr Thr Val Ser Val Phe 305 310 315 320 Ala Lys Ser Thr Gly Asp Pro Gln Gly Val Ile Arg His Tyr Val Val 325 330 335 Cys Ser Thr Pro Gln Ser Gln Tyr Tyr Leu Ala Glu Lys His Leu Phe 340 345 350 Ser Thr Ile Pro Glu Leu Ile Asn Tyr His Gln His Asn Ser Ala Gly 355 360 365 Leu Ile Ser Arg Leu Lys Tyr Pro Val Ser Gln Gln Asn Lys Asn Ala 370 375 380 Pro Ser Thr Ala Gly Leu Gly Tyr Gly Ser Trp Glu Ile Asp Pro Lys 385 390 395 400 Asp Leu Thr Phe Leu Lys Glu Leu Gly Thr Gly Gln Phe Gly Val Val 405 410 415 Lys Tyr Gly Lys Trp Arg Gly Gln Tyr Asp Val Ala Ile Lys Met Ile 420 425 430 Lys Glu Gly Ser Met Ser Glu Asp Glu Phe Ile Glu Glu Ala Lys Val 435 440 445 Met Met Asn Leu Ser His Glu Lys Leu Val Gln Leu Tyr Gly Val Cys 450 455 460 Thr Lys Gln Arg Pro Ile Phe Ile Ile Thr Glu Tyr Met Ala Asn Gly 465 470 475 480 Cys Leu Leu Asn Tyr Leu Arg Glu Met Arg His Arg Phe Gln Thr Gln 485 490 495 Gln Leu Leu Glu Met Cys Lys Asp Val Cys Glu Ala Met Glu Tyr Leu 500 505 510 Glu Ser Lys Gln Phe Leu His Arg Asp Leu Ala Ala Arg Asn Cys Leu 515 520 525 Val Asn Asp Gln Gly Val Val Lys Val Ser Asp Phe Gly Leu Ser Arg 530 535 540 Tyr Val Leu Asp Asp Glu Tyr Thr Ser Ser Val Gly Ser Lys Phe Pro 545 550 555 560 Val Arg Trp Ser Pro Pro Glu Val Leu Met Tyr Ser Lys Phe Ser Ser 565 570 575 Lys Ser Asp Ile Trp Ala Phe Gly Val Leu Met Trp Glu Ile Tyr Ser 580 585 590 Leu Gly Lys Met Pro Tyr Glu Arg Phe Thr Asn Ser Glu Thr Ala Glu 595 600 605 His Ile Ala Gln Gly Leu Arg Leu Tyr Arg Pro His Leu Ala Ser Glu 610 615 620 Lys Val Tyr Thr Ile Met Tyr Ser Cys Trp His Glu Lys Ala Asp Glu 625 630 635 640 Arg Pro Thr Phe Lys Ile Leu Leu Ser Asn Ile Leu Asp Val Met Asp 645 650 655 Glu Glu Ser < 210 > 115 < 211 > 225 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : SOCS 3 ; P 4030 without epitope tag < 400 > 115 Met Val Thr His Ser Lys Phe Pro Ala Ala Gly Met Ser Arg Pro Leu 1 5 10 15 Asp Thr Ser Leu Arg Leu Lys Thr Phe Ser Ser Lys Ser Glu Tyr Gln 20 25 30 Leu Val Val Asn Ala Val Arg Lys Leu Gln Glu Ser Gly Phe Tyr Trp 35 40 45 Ser Ala Val Thr Gly Gly Glu Ala Asn Leu Leu Leu Ser Ala Glu Pro 50 55 60 Ala Gly Thr Phe Leu Ile Arg Asp Ser Ser Asp Gln Arg His Phe Phe 65 70 75 80 Thr Leu Ser Val Lys Thr Gln Ser Gly Thr Lys Asn Leu Arg Ile Gln 85 90 95 Cys Glu Gly Gly Ser Phe Ser Leu Gln Ser Asp Pro Arg Ser Thr Gln 100 105 110 Pro Val Pro Arg Phe Asp Cys Val Leu Lys Leu Val His His Tyr Met 115 120 125 Pro Pro Pro Gly Ala Pro Ser Phe Pro Ser Pro Pro Thr Glu Pro Ser 130 135 140 Ser Glu Val Pro Glu Gln Pro Ser Ala Gln Pro Leu Pro Gly Ser Pro 145 150 155 160 Pro Arg Arg Ala Tyr Tyr Ile Tyr Ser Gly Gly Glu Lys Ile Pro Leu 165 170 175 Val Leu Ser Arg Pro Leu Ser Ser Asn Val Ala Thr Leu Gln His Leu 180 185 190 Cys Arg Lys Thr Val Asn Gly His Leu Asp Ser Tyr Glu Lys Val Thr 195 200 205 Gln Leu Pro Gly Pro Ile Arg Glu Phe Leu Asp Gln Tyr Asp Ala Pro 210 215 220 Leu 225 < 210 > 116 < 211 > 347 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 347 ) < 223 > IZ _ hsCASP 1 ( self - activating human Caspase 1 ) ; P 2024 without epitope tag < 400 > 116 Met Arg Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Leu Ser Lys 1 5 10 15 Ile Tyr His Ile Glu Asn Glu Ile Ala Arg Ile Lys Lys Leu Ile Gly 20 25 30 Glu Ala Asp Gln Thr Ser Gly Asn Tyr Leu Asn Met Gln Asp Ser Gln 35 40 45 Gly Val Leu Ser Ser Phe Pro Ala Pro Gln Ala Val Gln Asp Asn Pro 50 55 60 Ala Met Pro Thr Ser Ser Gly Ser Glu Gly Asn Val Lys Leu Cys Ser 65 70 75 80 Leu Glu Glu Ala Gln Arg Ile Trp Lys Gln Lys Ser Ala Glu Ile Tyr 85 90 95 Pro Ile Met Asp Lys Ser Ser Arg Thr Arg Leu Ala Leu Ile Ile Cys 100 105 110 Asn Glu Glu Phe Asp Ser Ile Pro Arg Arg Thr Gly Ala Glu Val Asp 115 120 125 Ile Thr Gly Met Thr Met Leu Leu Gln Asn Leu Gly Tyr Ser Val Asp 130 135 140 Val Lys Lys Asn Leu Thr Ala Ser Asp Met Thr Thr Glu Leu Glu Ala 145 150 155 160 Phe Ala His Arg Pro Glu His Lys Thr Ser Asp Ser Thr Phe Leu Val 165 170 175 Phe Met Ser His Gly Ile Arg Glu Gly Ile Cys Gly Lys Lys His Ser 180 185 190 Glu Gln Val Pro Asp Ile Leu Gln Leu Asn Ala Ile Phe Asn Met Leu 195 200 205 Asn Thr Lys Asn Cys Pro Ser Leu Lys Asp Lys Pro Lys Val Ile Ile 210 215 220 Ile Gln Ala Cys Arg Gly Asp Ser Pro Gly Val Val Trp Phe Lys Asp 225 230 235 240 Ser Val Gly Val Ser Gly Asn Leu Ser Leu Pro Thr Thr Glu Glu Phe 245 250 255 Glu Asp Asp Ala Ile Lys Lys Ala His Ile Glu Lys Asp Phe Ile Ala 260 265 270 Phe Cys Ser Ser Thr Pro Asp Asn Val Ser Trp Arg His Pro Thr Met 275 280 285 Gly Ser Val Phe Ile Gly Arg Leu Ile Glu His Met Gln Glu Tyr Ala 290 295 300 Cys Ser Cys Asp Val Glu Glu Ile Phe Arg Lys Val Arg Phe Ser Phe 305 310 315 320 Glu Gln Pro Asp Gly Arg Ala Gln Met Pro Thr Thr Glu Arg Val Thr 325 330 335 Leu Thr Arg Cys Phe Tyr Leu Phe Pro Gly His 340 345 < 210 > 117 < 211 > 347 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 347 ) < 223 > DM _ hsCASP 1 ( self - activating human Caspase 1 ) ; P 2025 without epitope tag < 400 > 117 Met Arg Met Lys Gln Leu Glu Asp Lys Ile Glu Glu Leu Leu Ser Lys 1 5 10 15 Ile Tyr His Leu Glu Asn Glu Ile Ala Arg Leu Lys Lys Leu Ile Gly 20 25 30 Glu Ala Asp Gln Thr Ser Gly Asn Tyr Leu Asn Met Gln Asp Ser Gln 35 40 45 Gly Val Leu Ser Ser Phe Pro Ala Pro Gln Ala Val Gln Asp Asn Pro 50 55 60 Ala Met Pro Thr Ser Ser Gly Ser Glu Gly Asn Val Lys Leu Cys Ser 65 70 75 80 Leu Glu Glu Ala Gln Arg Ile Trp Lys Gln Lys Ser Ala Glu Ile Tyr 85 90 95 Pro Ile Met Asp Lys Ser Ser Arg Thr Arg Leu Ala Leu Ile Ile Cys 100 105 110 Asn Glu Glu Phe Asp Ser Ile Pro Arg Arg Thr Gly Ala Glu Val Asp 115 120 125 Ile Thr Gly Met Thr Met Leu Leu Gln Asn Leu Gly Tyr Ser Val Asp 130 135 140 Val Lys Lys Asn Leu Thr Ala Ser Asp Met Thr Thr Glu Leu Glu Ala 145 150 155 160 Phe Ala His Arg Pro Glu His Lys Thr Ser Asp Ser Thr Phe Leu Val 165 170 175 Phe Met Ser His Gly Ile Arg Glu Gly Ile Cys Gly Lys Lys His Ser 180 185 190 Glu Gln Val Pro Asp Ile Leu Gln Leu Asn Ala Ile Phe Asn Met Leu 195 200 205 Asn Thr Lys Asn Cys Pro Ser Leu Lys Asp Lys Pro Lys Val Ile Ile 210 215 220 Ile Gln Ala Cys Arg Gly Asp Ser Pro Gly Val Val Trp Phe Lys Asp 225 230 235 240 Ser Val Gly Val Ser Gly Asn Leu Ser Leu Pro Thr Thr Glu Glu Phe 245 250 255 Glu Asp Asp Ala Ile Lys Lys Ala His Ile Glu Lys Asp Phe Ile Ala 260 265 270 Phe Cys Ser Ser Thr Pro Asp Asn Val Ser Trp Arg His Pro Thr Met 275 280 285 Gly Ser Val Phe Ile Gly Arg Leu Ile Glu His Met Gln Glu Tyr Ala 290 295 300 Cys Ser Cys Asp Val Glu Glu Ile Phe Arg Lys Val Arg Phe Ser Phe 305 310 315 320 Glu Gln Pro Asp Gly Arg Ala Gln Met Pro Thr Thr Glu Arg Val Thr 325 330 335 Leu Thr Arg Cys Phe Tyr Leu Phe Pro Gly His 340 345 < 210 > 118 < 211 > 346 < 212 > PRT < 213 > Mus musculus < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 346 ) < 223 > IZ _ mmCASP 1 ( self - activating mouse Caspase 1 ) ; P 2026 without epitope tag < 400 > 118 Met Arg Met Lys Gln Ile Glu Asp Lys Ile Glu Glu Ile Leu Ser Lys 1 5 10 15 Ile Tyr His Ile Glu Asn Glu Ile Ala Arg Ile Lys Lys Leu Ile Gly 20 25 30 Glu Arg Ser Ala Pro Ser Ala Glu Thr Phe Val Ala Thr Glu Asp Ser 35 40 45 Lys Gly Gly His Pro Ser Ser Ser Glu Thr Lys Glu Glu Gln Asn Lys 50 55 60 Glu Asp Gly Thr Phe Pro Gly Leu Thr Gly Thr Leu Lys Phe Cys Pro 65 70 75 80 Leu Glu Lys Ala Gln Lys Leu Trp Lys Glu Asn Pro Ser Glu Ile Tyr 85 90 95 Pro Ile Met Asn Thr Thr Thr Arg Thr Arg Leu Ala Leu Ile Ile Cys 100 105 110 Asn Thr Glu Phe Gln His Leu Ser Pro Arg Val Gly Ala Gln Val Asp 115 120 125 Leu Arg Glu Met Lys Leu Leu Leu Glu Asp Leu Gly Tyr Thr Val Lys 130 135 140 Val Lys Glu Asn Leu Thr Ala Leu Glu Met Val Lys Glu Val Lys Glu 145 150 155 160 Phe Ala Ala Cys Pro Glu His Lys Thr Ser Asp Ser Thr Phe Leu Val 165 170 175 Phe Met Ser His Gly Ile Gln Glu Gly Ile Cys Gly Thr Thr Tyr Ser 180 185 190 Asn Glu Val Ser Asp Ile Leu Lys Val Asp Thr Ile Phe Gln Met Met 195 200 205 Asn Thr Leu Lys Cys Pro Ser Leu Lys Asp Lys Pro Lys Val Ile Ile 210 215 220 Ile Gln Ala Cys Arg Gly Glu Lys Gln Gly Val Val Leu Leu Lys Asp 225 230 235 240 Ser Val Arg Asp Ser Glu Glu Asp Phe Leu Thr Asp Ala Ile Phe Glu 245 250 255 Asp Asp Gly Ile Lys Lys Ala His Ile Glu Lys Asp Phe Ile Ala Phe 260 265 270 Cys Ser Ser Thr Pro Asp Asn Val Ser Trp Arg His Pro Val Arg Gly 275 280 285 Ser Leu Phe Ile Glu Ser Leu Ile Lys His Met Lys Glu Tyr Ala Trp 290 295 300 Ser Cys Asp Leu Glu Asp Ile Phe Arg Lys Val Arg Phe Ser Phe Glu 305 310 315 320 Gln Pro Glu Phe Arg Leu Gln Met Pro Thr Ala Asp Arg Val Thr Leu 325 330 335 Thr Lys Arg Phe Tyr Leu Phe Pro Gly His 340 345 < 210 > 119 < 211 > 346 < 212 > PRT < 213 > Mus musculus < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 346 ) < 223 > DM _ mmCASP 1 ( self - activating mouse Caspase 1 ) ; P 2027 without epitope tag < 400 > 119 Met Arg Met Lys Gln Leu Glu Asp Lys Ile Glu Glu Leu Leu Ser Lys 1 5 10 15 Ile Tyr His Leu Glu Asn Glu Ile Ala Arg Leu Lys Lys Leu Ile Gly 20 25 30 Glu Arg Ser Ala Pro Ser Ala Glu Thr Phe Val Ala Thr Glu Asp Ser 35 40 45 Lys Gly Gly His Pro Ser Ser Ser Glu Thr Lys Glu Glu Gln Asn Lys 50 55 60 Glu Asp Gly Thr Phe Pro Gly Leu Thr Gly Thr Leu Lys Phe Cys Pro 65 70 75 80 Leu Glu Lys Ala Gln Lys Leu Trp Lys Glu Asn Pro Ser Glu Ile Tyr 85 90 95 Pro Ile Met Asn Thr Thr Thr Arg Thr Arg Leu Ala Leu Ile Ile Cys 100 105 110 Asn Thr Glu Phe Gln His Leu Ser Pro Arg Val Gly Ala Gln Val Asp 115 120 125 Leu Arg Glu Met Lys Leu Leu Leu Glu Asp Leu Gly Tyr Thr Val Lys 130 135 140 Val Lys Glu Asn Leu Thr Ala Leu Glu Met Val Lys Glu Val Lys Glu 145 150 155 160 Phe Ala Ala Cys Pro Glu His Lys Thr Ser Asp Ser Thr Phe Leu Val 165 170 175 Phe Met Ser His Gly Ile Gln Glu Gly Ile Cys Gly Thr Thr Tyr Ser 180 185 190 Asn Glu Val Ser Asp Ile Leu Lys Val Asp Thr Ile Phe Gln Met Met 195 200 205 Asn Thr Leu Lys Cys Pro Ser Leu Lys Asp Lys Pro Lys Val Ile Ile 210 215 220 Ile Gln Ala Cys Arg Gly Glu Lys Gln Gly Val Val Leu Leu Lys Asp 225 230 235 240 Ser Val Arg Asp Ser Glu Glu Asp Phe Leu Thr Asp Ala Ile Phe Glu 245 250 255 Asp Asp Gly Ile Lys Lys Ala His Ile Glu Lys Asp Phe Ile Ala Phe 260 265 270 Cys Ser Ser Thr Pro Asp Asn Val Ser Trp Arg His Pro Val Arg Gly 275 280 285 Ser Leu Phe Ile Glu Ser Leu Ile Lys His Met Lys Glu Tyr Ala Trp 290 295 300 Ser Cys Asp Leu Glu Asp Ile Phe Arg Lys Val Arg Phe Ser Phe Glu 305 310 315 320 Gln Pro Glu Phe Arg Leu Gln Met Pro Thr Ala Asp Arg Val Thr Leu 325 330 335 Thr Lys Arg Phe Tyr Leu Phe Pro Gly His 340 345 < 210 > 120 < 211 > 106 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : ADR concatemer with HIS tag < 400 > 120 Met His His His His His His His His His His Gly Lys Pro Ile Pro 1 5 10 15 Asn Pro Leu Leu Gly Leu Asp Ser Thr Gly Ile Pro Val His Leu Glu 20 25 30 Leu Ala Ser Met Thr Asn Met Glu Leu Met Ser Ser Ile Val His Gln 35 40 45 Gln Val Phe Pro Thr Glu Ala Gly Gln Ser Leu Val Ile Ser Ala Ser 50 55 60 Ile Ile Val Phe Asn Leu Leu Glu Leu Glu Gly Asp Tyr Arg Gly Arg 65 70 75 80 Val Leu Glu Leu Phe Arg Ala Ala Gln Leu Ala Asn Asp Val Val Leu 85 90 95 Gln Ile Met Glu Leu Cys Gly Ala Thr Arg 100 105 < 210 > 121 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 D 9 mer < 400 > 121 Val Val Gly Ala Asp Gly Val Gly Lys 1 5 < 210 > 122 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 V 9 mer < 400 > 122 Val Val Gly Ala Val Gly Val Gly Lys 1 5 < 210 > 123 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 13 D 9 mer < 400 > 123 Val Gly Ala Gly Asp Val Gly Lys Ser 1 5 < 210 > 124 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 C 9 mer < 400 > 124 Val Val Gly Ala Cys Gly Val Gly Lys 1 5 < 210 > 125 < 211 > 15 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 C 15 mer < 400 > 125 Met Lys Leu Val Val Val Gly Ala Cys Gly Val Gly Lys Ser Ala 1 5 10 15 < 210 > 126 < 211 > 75 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 D 25 mer nucleotide sequence < 400 > 126 atgaccgagt acaagctggt ggtggtgggc gccgacggcg tgggcaagag cgccctgacc 60 atccagctga tccag 75 < 210 > 127 < 211 > 75 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 V 25 mer nucleotide sequence < 400 > 127 atgaccgagt acaagctggt ggtggtgggc gccgtgggcg tgggcaagag cgccctgacc 60 atccagctga tccag 75 < 210 > 128 < 211 > 75 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 13 D 25 mer nucleotide sequence < 400 > 128 atgaccgagt acaagctggt ggtggtgggc gccggcgacg tgggcaagag cgccctgacc 60 atccagctga tccag 75 < 210 > 129 < 211 > 225 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 D 25 mer ^ 3 nucleotide sequence < 400 > 129 atgaccgagt acaagttagt ggttgtgggc gccgacggcg tgggcaagag cgccctcacc 60 atccagctta tccagatgac ggaatataag ttagtagtag tgggagccga cggtgtcggc 120 aagtccgctt tgaccattca acttattcag atgacagagt ataagctggt cgttgtaggc 180 gcagacggcg ttggaaagtc ggcactgacg atccagttga tccag 225 < 210 > 130 < 211 > 225 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 V 25 mer ^ 3 nucleotide sequence < 400 > 130 atgaccgagt acaagctcgt cgtggtgggc gccgtgggcg tgggcaagag cgccctaacc 60 atccagttga tccagatgac cgaatataag ctcgtggtag tcggagcggt gggcgttggc 120 aagtcagcgc taacaataca actaatccaa atgaccgaat acaagctagt tgtagtcggt 180 gccgtcggcg ttggaaagtc agcccttaca attcagctca ttcag 225 < 210 > 131 < 211 > 225 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 13 D 25 mer ^ 3 nucleotide sequence < 400 > 131 atgaccgagt acaagctcgt agtggttggc gccggcgacg tgggcaagag cgccctaacc 60 atccagctca tccagatgac agaatataag cttgtggttg tgggagcagg agacgtggga 120 aagagtgcgt tgacgattca actcatacag atgaccgaat acaagttggt ggtggtcggc 180 gcaggtgacg ttggtaagtc tgcactaact atacaactga tccag 225 < 210 > 132 < 211 > 75 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 C 25 mer nucleotide sequence < 400 > 132 atgaccgagt acaagctggt ggtggtgggc gcctgcggcg tgggcaagag cgccctgacc 60 atccagctga tccag 75 < 210 > 133 < 211 > 75 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS WT 25 mer nucleotide sequence < 400 > 133 atgaccgagt acaagctggt ggtggtgggc gccggcggcg tgggcaagag cgccctgacc 60 atccagctga tccag 75 < 210 > 134 < 211 > 47 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : 5 UTR sequence ; no promoter < 400 > 134 gggaaataag agagaaaaga agagtaagaa gaaatataag agccacc 47 < 210 > 135 < 211 > 75 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D G 12 V G 13 D ) 75 mer 3 MUT aa . seq < 400 > 135 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln Met Thr Glu Tyr Lys Leu Val 20 25 30 Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu Thr Ile Gln Leu 35 40 45 Ile Gln Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Asp Val 50 55 60 Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln 65 70 75 < 210 > 136 < 211 > 225 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D G 12 V G 13 D ) 75 mer 3 MUT nt . seq < 400 > 136 atgaccgagt acaagctcgt tgtagtcggc gccgacggcg tgggcaagag cgccttgacc 60 atccagttga tccagatgac cgaatataag ttggtggtgg taggcgcagt gggagttggc 120 aagtcagcac tcacaattca gctcattcaa atgacagaat acaagttagt cgttgtagga 180 gcaggcgacg tcggcaagag tgccttaacc attcaactaa tccag 225 < 210 > 137 < 211 > 100 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D G 12 V G 13 D G 12 C ) 100 mer 4 MUT aa . seq < 400 > 137 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Asp Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln Met Thr Glu Tyr Lys Leu Val 20 25 30 Val Val Gly Ala Val Gly Val Gly Lys Ser Ala Leu Thr Ile Gln Leu 35 40 45 Ile Gln Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Asp Val 50 55 60 Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln Met Thr Glu Tyr Lys 65 70 75 80 Leu Val Val Val Gly Ala Cys Gly Val Gly Lys Ser Ala Leu Thr Ile 85 90 95 Gln Leu Ile Gln 100 < 210 > 138 < 211 > 300 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D G 12 V G 13 D G 12 C ) 100 mer 4 MUT nt . seq < 400 > 138 atgaccgagt acaagctcgt ggtcgtcggc gccgacgggg taggcaagtc cgctctgacc 60 attcagctca tccagatgac ggagtacaaa ctcgtggtag tgggagccgt gggtgtgggc 120 aagagcgcgc tcaccatcca actcatccaa atgaccgaat ataaactcgt cgtggtggga 180 gccggcgacg tgggaaagag cgcccttacc atccagttaa tccagatgac agaatacaag 240 ctggtggtgg tcggtgcctg cggcgtgggt aagtccgccc tgacaatcca gctgatccag 300 < 210 > 139 < 211 > 1137 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : huSTING ( V 155 M ) ; no epitope tag ; nucleotide sequence < 400 > 139 atgccccaca gtagcctcca ccccagcatc ccctgcccca gaggccacgg cgcacagaag 60 gccgccctgg tgctgctgag cgcctgtctg gtgaccctgt ggggtctggg cgagcccccc 120 gagcacaccc tgcggtacct cgtgctgcat ctggccagcc tgcagctggg cctgctgctg 180 aacggcgtgt gcagcctggc cgaagagctg agacacatcc acagcagata cagaggctcc 240 tactggagaa ccgtcagagc ctgcctcggc tgtcccctga gaagaggcgc cctgctgctc 300 ctgagcatct acttctacta cagcctgccc aacgccgtgg gccccccctt cacctggatg 360 ctggccctgc tgggcctgag ccaggccctg aacatcctgc tgggcctgaa gggcttggcc 420 cccgccgaga tctccgccgt gtgcgagaag ggcaacttca acatggccca tggccttgcc 480 tggtcctact acatcggcta cctgagactg atcctgcccg agctgcaggc cagaatcaga 540 acctacaacc agcactacaa caacctgctg agaggcgccg tgagccaaag actgtacatc 600 ctgctgcccc tggactgcgg cgtgcccgac aaccttagca tggccgaccc caacatcaga 660 ttcctggaca agctgcccca gcagaccggc gaccacgccg gcatcaagga cagagtgtac 720 agcaacagca tctacgagct gctggagaac ggccagagag ccggcacctg cgtgctggag 780 tacgccaccc ccctgcagac cctgttcgcc atgagccagt acagccaggc cggcttcagc 840 agagaggaca gactggagca agccaagctg ttctgcagaa ccctggagga catcctggcg 900 gacgcccccg agagccaaaa caactgcaga ctgatcgcct accaggagcc cgccgacgac 960 agcagcttca gcctgagcca ggaagtgctg agacacctga gacaggaaga gaaggaggag 1020 gtgaccgtgg gaagcctgaa gaccagcgcc gtgcccagca ccagcaccat gagccaggag 1080 cccgagctgc tgatcagcgg catggagaag cccctgcccc tgagaaccga cttcagc 1137 < 210 > 140 < 211 > 1137 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 284 T ) ; no epitope tag ; nucleotide sequence < 400 > 140 atgcctcaca gcagcctgca ccctagcatc ccttgcccta gaggccacgg cgcccagaag 60 gccgccctgg tgctgctgag cgcctgcctg gtgaccctgt ggggcctggg cgagcctcct 120 gagcacaccc tgagatacct ggtgctgcac ctggccagcc tgcagctggg cctgctgctg 180 aacggcgtgt gcagcctggc cgaggagctg agacacatcc acagcagata cagaggcagc 240 tactggagaa ccgtgagagc ctgcctgggc tgccctctga gaagaggcgc cctgctgctg 300 ctgagcatct acttctacta cagcctgcct aacgccgtgg gccctccttt cacctggatg 360 ctggccctgc tgggcctgag ccaggccctg aacatcctgc tgggcctgaa gggcctggcc 420 cctgccgaga tcagcgccgt gtgcgagaag ggcaacttca acgtggccca cggcctggcc 480 tggagctact acatcggcta cctgagactg atcctgcctg agctgcaggc cagaatcaga 540 acctacaacc agcactacaa caacctgctg agaggcgccg tgagccagag actgtacatc 600 ctgctgcctc tggactgcgg cgtgcctgac aacctgagca tggccgaccc taacatcaga 660 ttcctggaca agctgcctca gcagaccggc gaccacgccg gcatcaagga cagagtgtac 720 agcaacagca tctacgagct gctggagaac ggccagagag ccggcacctg cgtgctggag 780 tacgccaccc ctctgcagac cctgttcgcc atgagccagt acagccaggc cggcttcagc 840 agagaggaca ccctggagca ggccaagctg ttctgcagaa ccctggagga catcctggcc 900 gacgcccctg agagccagaa caactgcaga ctgatcgcct accaggagcc tgccgacgac 960 agcagcttca gcctgagcca ggaggtgctg agacacctga gacaggagga gaaggaggag 1020 gtgaccgtgg gcagcctgaa gaccagcgcc gtgcctagca ccagcaccat gagccaggag 1080 cctgagctgc tgatcagcgg catggagaag cctctgcctc tgagaaccga cttcagc 1137 < 210 > 141 < 211 > 1137 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : hu STING ( R 284 M ) ; no epitope tag ; nucleotide sequence < 400 > 141 atgccccaca gcagcctgca cccctccatc ccctgtccca gaggccacgg cgcccagaag 60 gccgccctgg tgctgctgag cgcctgcctg gtgaccttat ggggcctggg cgagcccccc 120 gagcacaccc tgagatacct ggtcctgcac ctggccagcc tccagctggg cctgctgctc 180 aacggcgtgt gtagcctggc cgaggagctg agacacatcc acagcagata cagaggcagc 240 tactggagaa ccgtgagagc ctgcctgggt tgcccactga gaagaggagc tctgctgctg 300 ctgagcatct acttctacta ctcgctgccc aacgctgtgg gccccccctt cacctggatg 360 ctggccctgc tgggtctgag ccaggccctg aacatcctcc tgggcctgaa gggcctggcc 420 cccgccgaga taagcgccgt ttgcgagaag ggcaacttca acgtggccca tggcctggcc 480 tggagctact acatcggcta cttacgcctg atcctgcccg agctgcaggc cagaatcaga 540 acctacaacc agcattacaa caacctgctg agaggcgccg tgagccagag actgtatatc 600 ctgctgcccc tggactgcgg cgtgcccgac aacctgagca tggccgaccc caacatcaga 660 ttcctggaca agctccccca gcagaccggc gaccacgccg gaatcaaaga cagagtgtat 720 agcaacagca tctacgagct gctggagaac ggccagagag ccggcacctg cgtactggag 780 tacgccaccc ccttgcagac cctgtttgcc atgagccagt acagccaggc cggcttcagc 840 agagaggaca tgctggagca ggccaagctg ttctgcagaa ccctggagga catcctggcc 900 gacgcccccg agagccagaa caactgcaga ctgatcgcct accaagagcc cgccgacgac 960 agcagcttca gcttaagcca ggaggtgctg agacatctga gacaggagga gaaggaggag 1020 gtgaccgtgg gcagcctcaa gaccagcgct gtgccctcta ccagcaccat gagccaggag 1080 cccgagctgc tgatcagcgg catggagaag cccctgcccc tgagaacaga cttcagc 1137 < 210 > 142 < 211 > 1137 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 284 K ) ; no epitope tag ; nucleotide sequence < 400 > 142 atgccccata gcagcctgca ccccagcatc ccctgcccca gaggccacgg cgcccagaag 60 gccgccctgg tcctgctgag cgcatgcctg gtcaccctgt ggggcctggg cgagcccccc 120 gagcacaccc tgagatacct ggtgctgcac ctcgccagcc tgcagctggg cctgctgctg 180 aacggcgtgt gcagcctggc cgaggagctg agacacatcc acagcagata tagaggcagc 240 tactggagaa ccgtgagagc ttgcctcggc tgccccctga gaagaggcgc cctgctgctg 300 ctgagcatct acttttacta cagcctgccc aacgctgtgg gccccccttt cacgtggatg 360 ctcgccctgc tgggactgag ccaggccctg aacatcctgc tgggccttaa gggcctagcc 420 cccgccgaga tcagcgccgt gtgcgagaag ggcaacttca atgtggccca cggcctggcc 480 tggagctact acatcggcta cctgagactg atcctgcccg agctgcaggc cagaatcaga 540 acctacaatc agcactacaa caacctgctg agaggcgccg tgagccagag actgtacatc 600 ctgctgcccc tggactgcgg cgtgcccgac aacctcagca tggccgaccc caacatcaga 660 ttcctggaca agctgcccca gcagaccggc gaccacgccg gcatcaagga tcgcgtgtac 720 agcaacagca tctacgagct gctggaaaac ggccagagag ccggaacctg cgtgctggag 780 tacgccacac ccctgcagac cctgttcgcc atgagccagt acagccaggc cggcttcagc 840 agagaggaca agctggagca ggccaagctg ttctgcagaa ccctggagga tatcctcgcc 900 gacgcccccg agagccagaa caactgcagg ctgatcgcgt accaggagcc cgctgacgac 960 agcagcttta gcctgagcca ggaggtgctg agacatctgc gtcaagagga aaaggaggag 1020 gtgaccgtgg gctccctgaa gaccagcgcc gtgcccagca ccagcaccat gagccaggag 1080 cccgagctgc tgatcagcgg catggagaag ccactgcccc tcagaaccga cttcagc 1137 < 210 > 143 < 211 > 1137 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( N 154 S ) ; no epitope tag ; nucleotide sequence < 400 > 143 atgcctcaca gcagcctgca ccctagcatc ccttgcccta gaggccacgg cgcccagaag 60 gccgccctgg tgctgctgag cgcctgcctg gtgaccctgt ggggcctggg cgagcctcct 120 gagcacaccc tgagatacct ggtgctgcac ctggccagcc tgcagctggg cctgctgctg 180 aacggcgtgt gcagcctggc cgaggagctg agacacatcc acagcagata cagaggcagc 240 tactggagaa ccgtgagagc ctgcctgggc tgccctctga gaagaggcgc cctgctgctg 300 ctgagcatct acttctacta cagcctgcct aacgccgtgg gccctccttt cacctggatg 360 ctggccctgc tgggcctgag ccaggccctg aacatcctgc tgggcctgaa gggcctggcc 420 cctgccgaga tcagcgccgt gtgcgagaag ggcaacttca gcgtggccca cggcctggcc 480 tggagctact acatcggcta cctgagactg atcctgcctg agctgcaggc cagaatcaga 540 acctacaacc agcactacaa caacctgctg agaggcgccg tgagccagag actgtacatc 600 ctgctgcctc tggactgcgg cgtgcctgac aacctgagca tggccgaccc taacatcaga 660 ttcctggaca agctgcctca gcagaccggc gaccacgccg gcatcaagga cagagtgtac 720 agcaacagca tctacgagct gctggagaac ggccagagag ccggcacctg cgtgctggag 780 tacgccaccc ctctgcagac cctgttcgcc atgagccagt acagccaggc cggcttcagc 840 agagaggaca gactggagca ggccaagctg ttctgcagaa ccctggagga catcctggcc 900 gacgcccctg agagccagaa caactgcaga ctgatcgcct accaggagcc tgccgacgac 960 agcagcttca gcctgagcca ggaggtgctg agacacctga gacaggagga gaaggaggag 1020 gtgaccgtgg gcagcctgaa gaccagcgcc gtgcctagca ccagcaccat gagccaggag 1080 cctgagctgc tgatcagcgg catggagaag cctctgcctc tgagaaccga cttcagc 1137 < 210 > 144 < 211 > 1137 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( V 147 L ) ; no epitope tag ; nucleotide sequence < 400 > 144 atgcctcaca gcagcctgca ccctagcatc ccttgcccta gaggccacgg cgcccagaag 60 gccgccctgg tgctgctgag cgcctgcctg gtgaccctgt ggggcctggg cgagcctcct 120 gagcacaccc tgagatacct ggtgctgcac ctggccagcc tgcagctggg cctgctgctg 180 aacggcgtgt gcagcctggc cgaggagctg agacacatcc acagcagata cagaggcagc 240 tactggagaa ccgtgagagc ctgcctgggc tgccctctga gaagaggcgc cctgctgctg 300 ctgagcatct acttctacta cagcctgcct aacgccgtgg gccctccttt cacctggatg 360 ctggccctgc tgggcctgag ccaggccctg aacatcctgc tgggcctgaa gggcctggcc 420 cctgccgaga tcagcgccct gtgcgagaag ggcaacttca acgtggccca cggcctggcc 480 tggagctact acatcggcta cctgagactg atcctgcctg agctgcaggc cagaatcaga 540 acctacaacc agcactacaa caacctgctg agaggcgccg tgagccagag actgtacatc 600 ctgctgcctc tggactgcgg cgtgcctgac aacctgagca tggccgaccc taacatcaga 660 ttcctggaca agctgcctca gcagaccggc gaccacgccg gcatcaagga cagagtgtac 720 agcaacagca tctacgagct gctggagaac ggccagagag ccggcacctg cgtgctggag 780 tacgccaccc ctctgcagac cctgttcgcc atgagccagt acagccaggc cggcttcagc 840 agagaggaca gactggagca ggccaagctg ttctgcagaa ccctggagga catcctggcc 900 gacgcccctg agagccagaa caactgcaga ctgatcgcct accaggagcc tgccgacgac 960 agcagcttca gcctgagcca ggaggtgctg agacacctga gacaggagga gaaggaggag 1020 gtgaccgtgg gcagcctgaa gaccagcgcc gtgcctagca ccagcaccat gagccaggag 1080 cctgagctgc tgatcagcgg catggagaag cctctgcctc tgagaaccga cttcagc 1137 < 210 > 145 < 211 > 1137 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( E 315 Q ) ; no epitope tag ; nucleotide sequence < 400 > 145 atgcctcaca gcagcctgca ccctagcatc ccttgcccta gaggccacgg cgcccagaag 60 gccgccctgg tgctgctgag cgcctgcctg gtgaccctgt ggggcctggg cgagcctcct 120 gagcacaccc tgagatacct ggtgctgcac ctggccagcc tgcagctggg cctgctgctg 180 aacggcgtgt gcagcctggc cgaggagctg agacacatcc acagcagata cagaggcagc 240 tactggagaa ccgtgagagc ctgcctgggc tgccctctga gaagaggcgc cctgctgctg 300 ctgagcatct acttctacta cagcctgcct aacgccgtgg gccctccttt cacctggatg 360 ctggccctgc tgggcctgag ccaggccctg aacatcctgc tgggcctgaa gggcctggcc 420 cctgccgaga tcagcgccgt gtgcgagaag ggcaacttca acgtggccca cggcctggcc 480 tggagctact acatcggcta cctgagactg atcctgcctg agctgcaggc cagaatcaga 540 acctacaacc agcactacaa caacctgctg agaggcgccg tgagccagag actgtacatc 600 ctgctgcctc tggactgcgg cgtgcctgac aacctgagca tggccgaccc taacatcaga 660 ttcctggaca agctgcctca gcagaccggc gaccacgccg gcatcaagga cagagtgtac 720 agcaacagca tctacgagct gctggagaac ggccagagag ccggcacctg cgtgctggag 780 tacgccaccc ctctgcagac cctgttcgcc atgagccagt acagccaggc cggcttcagc 840 agagaggaca gactggagca ggccaagctg ttctgcagaa ccctggagga catcctggcc 900 gacgcccctg agagccagaa caactgcaga ctgatcgcct accagcagcc tgccgacgac 960 agcagcttca gcctgagcca ggaggtgctg agacacctga gacaggagga gaaggaggag 1020 gtgaccgtgg gcagcctgaa gaccagcgcc gtgcctagca ccagcaccat gagccaggag 1080 cctgagctgc tgatcagcgg catggagaag cctctgcctc tgagaaccga cttcagc 1137 < 210 > 146 < 211 > 1137 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 375 A ) ; no epitope tag ; nucleotide sequence < 400 > 146 atgcctcaca gcagcctgca ccctagcatc ccttgcccta gaggccacgg cgcccagaag 60 gccgccctgg tgctgctgag cgcctgcctg gtgaccctgt ggggcctggg cgagcctcct 120 gagcacaccc tgagatacct ggtgctgcac ctggccagcc tgcagctggg cctgctgctg 180 aacggcgtgt gcagcctggc cgaggagctg agacacatcc acagcagata cagaggcagc 240 tactggagaa ccgtgagagc ctgcctgggc tgccctctga gaagaggcgc cctgctgctg 300 ctgagcatct acttctacta cagcctgcct aacgccgtgg gccctccttt cacctggatg 360 ctggccctgc tgggcctgag ccaggccctg aacatcctgc tgggcctgaa gggcctggcc 420 cctgccgaga tcagcgccgt gtgcgagaag ggcaacttca acgtggccca cggcctggcc 480 tggagctact acatcggcta cctgagactg atcctgcctg agctgcaggc cagaatcaga 540 acctacaacc agcactacaa caacctgctg agaggcgccg tgagccagag actgtacatc 600 ctgctgcctc tggactgcgg cgtgcctgac aacctgagca tggccgaccc taacatcaga 660 ttcctggaca agctgcctca gcagaccggc gaccacgccg gcatcaagga cagagtgtac 720 agcaacagca tctacgagct gctggagaac ggccagagag ccggcacctg cgtgctggag 780 tacgccaccc ctctgcagac cctgttcgcc atgagccagt acagccaggc cggcttcagc 840 agagaggaca gactggagca ggccaagctg ttctgcagaa ccctggagga catcctggcc 900 gacgcccctg agagccagaa caactgcaga ctgatcgcct accaggagcc tgccgacgac 960 agcagcttca gcctgagcca ggaggtgctg agacacctga gacaggagga gaaggaggag 1020 gtgaccgtgg gcagcctgaa gaccagcgcc gtgcctagca ccagcaccat gagccaggag 1080 cctgagctgc tgatcagcgg catggagaag cctctgcctc tggccaccga cttcagc 1137 < 210 > 147 < 211 > 1137 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( V 147 L / N 154 S / V 155 M ) ; no epitope tag ; nucleotide sequence < 400 > 147 atgcctcaca gcagcctgca ccctagcatc ccttgcccta gaggccacgg cgcccagaag 60 gccgccctgg tgctgctgag cgcctgcctg gtgaccctgt ggggcctggg cgagcctcct 120 gagcacaccc tgagatacct ggtgctgcac ctggccagcc tgcagctggg cctgctgctg 180 aacggcgtgt gcagcctggc cgaggagctg agacacatcc acagcagata cagaggcagc 240 tactggagaa ccgtgagagc ctgcctgggc tgccctctga gaagaggcgc cctgctgctg 300 ctgagcatct acttctacta cagcctgcct aacgccgtgg gccctccttt cacctggatg 360 ctggccctgc tgggcctgag ccaggccctg aacatcctgc tgggcctgaa gggcctggcc 420 cctgccgaga tcagcgccct gtgcgagaag ggcaacttca gcatggccca cggcctggcc 480 tggagctact acatcggcta cctgagactg atcctgcctg agctgcaggc cagaatcaga 540 acctacaacc agcactacaa caacctgctg agaggcgccg tgagccagag actgtacatc 600 ctgctgcctc tggactgcgg cgtgcctgac aacctgagca tggccgaccc taacatcaga 660 ttcctggaca agctgcctca gcagaccggc gaccacgccg gcatcaagga cagagtgtac 720 agcaacagca tctacgagct gctggagaac ggccagagag ccggcacctg cgtgctggag 780 tacgccaccc ctctgcagac cctgttcgcc atgagccagt acagccaggc cggcttcagc 840 agagaggaca gactggagca ggccaagctg ttctgcagaa ccctggagga catcctggcc 900 gacgcccctg agagccagaa caactgcaga ctgatcgcct accaggagcc tgccgacgac 960 agcagcttca gcctgagcca ggaggtgctg agacacctga gacaggagga gaaggaggag 1020 gtgaccgtgg gcagcctgaa gaccagcgcc gtgcctagca ccagcaccat gagccaggag 1080 cctgagctgc tgatcagcgg catggagaag cctctgcctc tgagaaccga cttcagc 1137 < 210 > 148 < 211 > 1137 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 284 M / V 147 L / N 154 S / V 155 M ) ; no epitope tag ; nucleotide sequence < 400 > 148 atgcctcaca gcagcctgca ccctagcatc ccttgcccta gaggccacgg cgcccagaag 60 gccgccctgg tgctgctgag cgcctgcctg gtgaccctgt ggggcctggg cgagcctcct 120 gagcacaccc tgagatacct ggtgctgcac ctggccagcc tgcagctggg cctgctgctg 180 aacggcgtgt gcagcctggc cgaggagctg agacacatcc acagcagata cagaggcagc 240 tactggagaa ccgtgagagc ctgcctgggc tgccctctga gaagaggcgc cctgctgctg 300 ctgagcatct acttctacta cagcctgcct aacgccgtgg gccctccttt cacctggatg 360 ctggccctgc tgggcctgag ccaggccctg aacatcctgc tgggcctgaa gggcctggcc 420 cctgccgaga tcagcgccct gtgcgagaag ggcaacttca gcatggccca cggcctggcc 480 tggagctact acatcggcta cctgagactg atcctgcctg agctgcaggc cagaatcaga 540 acctacaacc agcactacaa caacctgctg agaggcgccg tgagccagag actgtacatc 600 ctgctgcctc tggactgcgg cgtgcctgac aacctgagca tggccgaccc taacatcaga 660 ttcctggaca agctgcctca gcagaccggc gaccacgccg gcatcaagga cagagtgtac 720 agcaacagca tctacgagct gctggagaac ggccagagag ccggcacctg cgtgctggag 780 tacgccaccc ctctgcagac cctgttcgcc atgagccagt acagccaggc cggcttcagc 840 agagaggaca tgctggagca ggccaagctg ttctgcagaa ccctggagga catcctggcc 900 gacgcccctg agagccagaa caactgcaga ctgatcgcct accaggagcc tgccgacgac 960 agcagcttca gcctgagcca ggaggtgctg agacacctga gacaggagga gaaggaggag 1020 gtgaccgtgg gcagcctgaa gaccagcgcc gtgcctagca ccagcaccat gagccaggag 1080 cctgagctgc tgatcagcgg catggagaag cctctgcctc tgagaaccga cttcagc 1137 < 210 > 149 < 211 > 141 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : 3 UTR used in STING V 155 M construct , containing miR 122 binding site < 400 > 149 tgataatagg ctggagcctc ggtggcctag cttcttgccc cttgggcctc cccccagccc 60 ctcctcccct tcctgcaccc gtacccccca aacaccattg tcacactcca gtggtctttg 120 aataaagtct gagtgggcgg c 141 < 210 > 150 < 211 > 1257 < 212 > DNA < 213 > Mus musculus < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1257 ) < 223 > super mouse IRF 3 S 396 D ; no epitope tag < 400 > 150 atggagaccc ccaagcctag aatcctgccc tggctggtga gccagctgga cctgggccag 60 ctggagggcg tagcctggct ggacgagagc agaaccagat tcagaatccc ctggaagcac 120 ggcctgagac aagacgccca gatggccgac ttcggcatct tccaggcctg ggccgaggcc 180 agcggcgcct acacccctgg caaggataag cccgatgtga gcacctggaa gagaaacttc 240 agaagcgccc tgaacagaaa ggaggtgctg agactggccg ccgacaatag caaggacccc 300 tacgaccccc acaaggtgta cgagttcgtt acccccggcg ccagggactt cgtgcacctg 360 ggcgccagcc ccgacaccaa cggcaagagc agcctgcccc acagccagga gaacctgccc 420 aagctgttcg atggcctgat cctgggcccc ctgaaggacg agggcagcag cgacctggcc 480 atcgtgagcg accctagcca gcagctgccc tcccccaacg tgaacaactt cctgaacccc 540 gccccccagg agaaccccct gaagcaactg ctggccgagg agcagtggga gttcgaggtg 600 accgccttct acagaggcag acaggtgttc cagcagaccc tgttctgccc cggcggcctg 660 agactggtag gcagcaccgc tgacatgacc ctgccctggc agcccgtgac cctgcccgac 720 cccgaaggct ttctgaccga caagctggtg aaggagtacg tcggccaagt gctgaagggc 780 ctgggcaacg gcctggccct gtggcaggcc ggccagtgcc tgtgggccca gagactcggc 840 cacagccacg ccttctgggc cctgggcgag gaactcctgc ccgatagcgg cagaggcccc 900 gacggcgagg tgcacaagga caaggacggc gccgtgttcg acctgcgccc cttcgtggcc 960 gacctgatcg ccttcatgga gggcagcggc cacagcccca gatataccct gtggttctgc 1020 atgggcgaga tgtggcccca ggaccagccc tgggtgaaga gactggtgat ggtgaaggtg 1080 gtgcccacct gcctgaaaga gctgctggag atggccagag agggcggcgc cagctccctg 1140 aaaaccgtgg acctgcacat tgacaacagc cagcccatca gcctgaccag cgaccagtac 1200 aaggcctacc tgcaggacct ggtggaggac atggacttcc aggccaccgg caacatc 1257 < 210 > 151 < 211 > 1281 < 212 > DNA < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1281 ) < 223 > super human IRF 3 S 396 D ; no epitope tag < 400 > 151 atgggcaccc ccaagcccag aatcctgccc tggctggtga gccagctgga cctgggccag 60 ctggagggag tggcctgggt gaacaagagc agaaccagat tcagaatccc ctggaagcac 120 ggcctcagac aggacgccca gcaggaggac ttcggcattt ttcaggcttg ggccgaggcc 180 accggcgcct acgtgcccgg cagagacaag cccgacctgc ccacctggaa aagaaacttc 240 agaagcgcct tgaatagaaa ggagggcctg agactggccg aggacagaag caaggacccc 300 cacgaccctc acaagatcta cgagttcgtg aatagcggcg tgggcgactt tagccagccc 360 gacaccagcc ccgacaccaa cggcggcggc agcaccagcg acacgcagga ggacatcctg 420 gatgaactgc tgggcaacat ggtgctggcc cccctgcccg atcccggccc cccttcgctt 480 gccgtggccc ccgagccctg cccccagccc ctgagaagcc cctctctgga taaccccacc 540 cccttcccca acctgggccc cagcgagaat ccactgaaga gacttctggt ccccggcgag 600 gagtgggagt tcgaggtgac cgccttctac agaggcagac aggtgttcca gcagaccatc 660 agctgccccg aaggcctgag attagtgggc agcgaagtgg gcgacaggac cctgcccggg 720 tggcccgtga ccctgcccga tcccggcatg agcctgaccg acagaggtgt gatgagctac 780 gtgagacacg tgctgagctg cctgggcggc ggcctggcac tgtggagagc cggccagtgg 840 ctgtgggccc agagactggg ccactgccac acctactggg ccgtgagcga ggagctgctg 900 cccaacagcg gccacggccc cgacggcgag gtgcccaagg acaaggaagg gggcgtgttc 960 gacctgggcc ccttcatcgt agacctgatc acctttaccg agggcagcgg caggagcccc 1020 agatacgccc tgtggttctg cgtgggcgaa agctggcccc aggaccagcc ctggaccaag 1080 agactggtga tggtgaaggt agtgcccacc tgcctgagag ccttagtgga gatggccaga 1140 gtgggcgggg ccagcagcct ggagaacacc gtggatcttc acatcgacaa cagccacccc 1200 ctgagcctga ccagcgacca gtacaaggcc tacctgcagg acctggtgga gggcatggac 1260 ttccagggcc ccggcgagac c 1281 < 210 > 152 < 211 > 1509 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Wild - type Hu IRF 7 isoform A ; P 037 without epitope tag < 400 > 152 atggcgctgg cccccgaaag agccgccccc agagtcctct tcggcgaatg gctccttggc 60 gaaatttcgt cgggctgcta cgagggctta caatggctgg atgaggcgag aacctgtttc 120 agggtgccct ggaaacactt cgccagaaag gatctaagcg aagcagatgc tagaattttt 180 aaggcttggg ccgtggccag gggaagatgg cccccctcga gcagaggcgg cggccctccc 240 cccgaggcag aaacggccga gagagccgga tggaaaacca atttcagatg cgccctgaga 300 tctacaagaa gattcgtgat gcttagagac aacagcggag atcccgccga tccccataag 360 gtgtatgccc tgtcccggga gctgtgctgg agggaagggc ctggcactga ccagaccgaa 420 gccgaagccc ccgcggccgt gccgccgccc caaggaggcc caccaggccc tttcctcgct 480 cacacccacg ccggtctgca agccccggga cctctacctg cccctgccgg cgataaaggc 540 gacctgttgc tgcaggccgt ccaacagagc tgcctggccg atcatctgct cacagccagc 600 tggggcgctg accccgtccc aacaaaggcc cccggtgagg gccaagaagg cctgcctctg 660 accggcgcct gtgccggcgg ccctggcctg cctgctggcg agctgtacgg atgggctgtc 720 gaaaccactc cctcccccgg cccccaacct gcggccctga caaccggcga ggcagccgca 780 cccgaaagcc cccaccaggc cgaaccctac ctcagtccca gcccctccgc ctgcaccgct 840 gtgcaggagc ccagccccgg tgctctggac gtaacaatca tgtacaaagg cagaaccgtg 900 cttcagaagg tggttggaca cccctcctgt acttttctct acggcccccc cgaccctgcc 960 gtgagagcta ccgacccgca acaggtggcc tttccctcgc ccgccgaact gcccgatcaa 1020 aaacagctga gatacaccga ggagctgctg agacacgtgg cgccgggctt acacctagag 1080 ttgagaggcc cccaactctg ggccagacgc atgggcaagt gtaaggtgta ctgggaggtc 1140 gggggccctc ccggctctgc cagccccagc acccctgctt gtctcttgcc cagaaactgt 1200 gataccccca tcttcgactt ccgtgtattt ttccaggaac tggtcgagtt tagagccaga 1260 cagagacgag gcagccccag atatacaatc tacctcggct tcggccagga cctgagtgcc 1320 ggcagaccta aggagaagtc gctggtccta gtgaagttag agccctggct atgtagagtg 1380 cacctggagg gcacccagag agaaggagtg agcagcctgg acagcagcag cctgagtctg 1440 tgcctgagct ccgccaactc gctgtatgat gacatcgagt gtttcctcat ggagctggag 1500 cagcccgcc 1509 < 210 > 153 < 211 > 1509 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active Hu IRF 7 S 477 D / S 479 D ; P 033 without epitope tag < 400 > 153 atggcccttg cccctgagcg ggccgccccc agagtgttat tcggcgagtg gctgctgggc 60 gagatcagca gcggctgcta cgagggactg cagtggctgg acgaggctag aacctgcttc 120 agagtgccct ggaagcattt cgccagaaaa gacctgagcg aggctgatgc tagaatcttc 180 aaagcctggg ctgtggcccg aggaagatgg ccccccagca gcagaggagg cggccctcct 240 cccgaggccg aaaccgcaga gcgtgctggc tggaaaacca actttaggtg tgccctgagg 300 agcaccagaa gattcgttat gctcagagac aacagcgggg accccgccga cccgcacaag 360 gtgtacgcct taagtaggga gctgtgctgg agagagggac cggggaccga ccaaaccgag 420 gctgaggcgc ccgccgccgt tccacctccc cagggtggtc ccccagggcc ctttctggca 480 cacacccacg ccggattaca ggcgccaggg cccttacccg cccccgccgg agacaaaggc 540 gacctcctgc tgcaagccgt gcaacaaagc tgcctggccg atcacttact aaccgctagc 600 tggggcgccg atcctgttcc caccaaggcc cccggtgaag ggcaagaagg actgccctta 660 accggcgcct gtgccggagg ccctggtctg ccagccggcg agctgtacgg ttgggctgtc 720 gaaacaacac ccagtccggg cccacagcct gccgctctga ccaccggcga agccgccgcc 780 cccgagagcc cacaccaggc tgaaccctac ctgagcccca gccccagcgc ctgcaccgct 840 gtgcaggagc ctagccccgg cgctcttgat gtgacaataa tgtacaaggg caggaccgtg 900 ctgcaaaagg tcgtgggcca tccgtcgtgt acctttctgt acggccctcc agaccccgcg 960 gttagagcca ccgaccccca gcaagtcgcc ttcccctccc ccgccgaact gcccgaccaa 1020 aagcagctgc ggtacacaga agaactactt agacacgtgg cccccggtct gcacttggag 1080 ctgagaggcc cccagctctg ggccagaaga atgggcaagt gcaaagtgta ctgggaggtg 1140 ggcggcccac ccggctcagc ttcgccctcc acacccgcat gcctgctgcc cagaaattgc 1200 gacacgccca tcttcgattt tagagtgttc tttcaggagt tggtggagtt cagagccaga 1260 caaagacgcg gcagccccag atacaccatt tacctcggct tcggccagga cctcagcgct 1320 ggcagaccca aggagaagag tctggtcctc gtgaagctgg agccctggct gtgcagagtg 1380 cacctggagg gcacccagcg tgaaggcgtg agcagcctgg attcaagcga cctggaccta 1440 tgcctaagca gcgctaactc actgtacgac gatatcgaat gcttcctgat ggaactggag 1500 cagcctgcc 1509 < 210 > 154 < 211 > 1509 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active Hu IRF 7 S 475 D / S 477 D / L 480 D ; P 034 without epitope tag < 400 > 154 atggccctgg cacccgagag ggccgccccc agggtgctct tcggcgagtg gttactaggc 60 gaaattagca gcggctgcta tgaaggcctt cagtggctgg acgaggccag aacctgcttt 120 agagttccct ggaagcactt cgcccggaaa gatctctctg aagccgacgc cagaatattc 180 aaggcctggg ctgtcgccag gggcaggtgg ccaccctcca gccgaggtgg cggccctccc 240 cctgaggctg agactgcgga aagggcgggc tggaagacca atttcagatg cgctctgaga 300 agcaccagac gttttgtgat gctaagagac aatagcggcg atcccgccga cccccataag 360 gtatacgcac tgagccgaga gctctgttgg agagaaggcc ccggcaccga ccagaccgag 420 gctgaagccc ctgcagccgt gcccccccct caaggcgggc cccccggccc cttcctggcc 480 catacccatg cagggttaca agcacccggg cccttgcccg ccccagcggg agacaagggc 540 gacctcttac tgcaggccgt gcaacaaagt tgtctggcgg accacctgct gaccgcatca 600 tggggcgcgg atcctgtgcc caccaaggca cccggcgaag gccaggaggg cctgcccttg 660 accggcgcct gcgctggcgg acccggccta cctgctggcg aactgtatgg ctgggccgta 720 gagacgactc ccagccctgg cccacaaccc gcggctttga ccaccggcga agccgccgcc 780 cccgagtctc cgcaccaggc cgagccttac ctcagcccaa gccctagcgc ctgcaccgcc 840 gtgcaagaac ctagccccgg agccctggat gtgacaatca tgtacaaggg tagaaccgta 900 ctgcaaaagg tggtgggtca tcccagctgc acctttcttt acggcccacc cgaccctgcc 960 gtgcgagcca cagacccaca acaggtcgcc ttcccaagcc ccgccgaact gcccgatcag 1020 aaacagctga gatatacaga ggagcttctg cggcacgtag ctcccggcct acatctcgag 1080 ctgaggggcc cacaactgtg ggccagacgc atgggcaaat gcaaggtcta ctgggaagtg 1140 ggaggccccc ccggcagcgc atctcccagc acgcccgcgt gcctgctgcc tagaaattgc 1200 gacaccccca tctttgactt ccgggtattc tttcaggagc tggtagagtt cagagccagg 1260 cagcggaggg gctcccccag atacacaatc tacctgggct tcggacagga cctgtccgcc 1320 ggccgcccca aggaaaagag cctggtgctg gtgaagctgg agccctggct gtgtagggta 1380 cacctcgaag gcacccagag agaaggagtg agctcgcttg atgacagcga tctgtcggat 1440 tgccttagca gcgccaacag cctgtatgat gatatcgagt gcttccttat ggaactggag 1500 cagcccgcc 1509 < 210 > 155 < 211 > 1509 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active Hu IRF 7 S 475 D / S 476 D / S 477 D / S 479 D / S 483 D / S 487 D ; P 035 without epitope tag < 400 > 155 atggccctag cccccgaaag agcagctccc agagtgctgt tcggcgaatg gctgcttggc 60 gagatcagca gcggctgcta cgaaggcctg cagtggctgg acgaagcccg cacctgtttc 120 agagtgccct ggaagcactt cgctagaaag gatttgagcg aggctgatgc tagaatcttt 180 aaggcttggg ctgtggcaag aggcagatgg ccgcctagta gcagaggggg cggacctccc 240 cccgaggctg agaccgctga gagagcaggg tggaaaacca acttcagatg cgcgctgaga 300 agcacccgaa gattcgtgat gctacgtgac aatagcggcg accccgccga cccccacaaa 360 gtgtacgccc tgtcccgaga actttgctgg agagagggac ccggcaccga tcaaacagag 420 gctgaggccc cggccgctgt acccccgccc caaggaggcc ccccaggccc ctttctggct 480 catacacatg ccggcctgca ggcacccggg cccctcccgg ctcctgccgg cgacaagggc 540 gatctccttc tccaggccgt gcagcagagc tgcctggccg atcacctgct gaccgcctcg 600 tggggcgccg accccgtgcc caccaaagcc ccgggtgaag gccaagaggg gctcccttta 660 accggagcat gcgccggagg ccccggcctg ccagccggcg agttatatgg ctgggctgtg 720 gagaccacac cctcccccgg ccctcaaccc gctgccctga ccaccggtga ggccgccgcc 780 cccgagagcc cacaccaggc cgaaccctac ctgagcccta gccctagcgc ctgcaccgcc 840 gtgcaagaac ccagccccgg agccctggat gtgaccatta tgtacaaggg ccggacagtg 900 ctgcaaaagg ttgtgggaca cccgagctgc acctttctgt acggtccgcc tgaccccgcc 960 gtgagagcca cggacccgca gcaggtggcc ttcccctcac ccgcggagct gcccgaccaa 1020 aagcaactca gatacacaga agaactattg cgtcacgtcg cgcccggcct gcatctggag 1080 ctgagaggcc cccagctctg ggccagaagg atgggcaaat gcaaggtgta ctgggaggtg 1140 ggaggccccc ccggcagcgc cagccccagc actcccgcgt gcctgctgcc cagaaattgc 1200 gacactccca tcttcgattt cagggtgttc ttccaggagc tggtggagtt cagagccagg 1260 cagagaaggg gtagccccag atacacaatc tatctaggct ttggacaaga tctgagcgcc 1320 ggccggccta aggaaaaaag cctggtgctg gtaaagctgg agccgtggct ttgtagagtg 1380 cacctggagg ggacgcagcg agagggcgtg agcagcttag acgacgatga cttggatctg 1440 tgtctcgaca gcgccaacga cttgtacgac gacatcgagt gcttcctgat ggaactggag 1500 cagcccgcc 1509 < 210 > 156 < 211 > 846 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active truncated Hu IRF 7 1 - 246 + 468 - 503 ; P 032 without epitope tag < 400 > 156 atggccctgg cccccgagag agccgccccc agagtgctct tcggcgagtg gctgctgggc 60 gagataagca gcggctgcta cgaaggtctg cagtggctag acgaggccag aacctgcttt 120 agagtgccct ggaagcactt cgctcgaaag gacctgtccg aggccgatgc tagaattttt 180 aaggcttggg ccgtcgctag gggaagatgg ccccctagca gtagaggcgg cggcccccct 240 cccgaagccg agacggccga gagggccggc tggaaaacca atttcagatg cgccctgagg 300 agcacccgca ggttcgtaat gctgcgagac aatagcggcg atcctgcgga tcctcacaag 360 gtttacgcct tgagtagaga actgtgctgg cgggagggcc ccggaaccga ccagacggag 420 gcagaggcac ccgctgccgt gcccccccct caaggaggac cccctggacc ctttctggcc 480 cacacccacg ctggtctgca ggccccaggc ccactgcccg ccccagcggg cgataagggt 540 gacctgctcc tacaggcggt gcaacagagc tgtctggccg accacctgtt gaccgccagc 600 tggggggccg acccggtgcc caccaaagct cccggagagg gccaagaagg cctcccacta 660 actggcgcct gcgccggggg cccgggatta cccgccggcg agctgtatgg ctgggccgtg 720 gagaccacgc ccagccccga gggcgtgtcg tccctggaca gcagcagcct gagcctgtgc 780 ctgagctccg ccaacagcct gtatgacgac atcgagtgct tcctgatgga gctggaacaa 840 cccgcc 846 < 210 > 157 < 211 > 846 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active truncated Hu IRF 7 1 - 246 + 468 - 503 plus S 475 D / S 476 D / S 477 D / S 479 D / S 483 D / S 487 D ; P 036 without epitope tag < 400 > 157 atggcactgg cgcctgaaag agccgctccg cgtgtgctct tcggcgagtg gctgctgggc 60 gagatcagct ccggctgcta cgagggtcta cagtggctgg acgaggccag aacctgtttt 120 agagtgccct ggaagcactt cgcgagaaag gacctgagcg aggccgacgc cagaatcttc 180 aaagcctggg cagtggctag gggcagatgg cctcccagca gccggggcgg cggcccaccc 240 cccgaggccg aaaccgccga aagagctggc tggaagacca acttcagatg cgccctgaga 300 agcaccagaa gatttgtcat gctgagagat aattcaggag accccgccga ccctcacaag 360 gtgtacgccc tgtccagaga gctgtgttgg agagagggcc ccggaaccga ccagaccgag 420 gccgaggctc cagctgccgt gccacccccc caaggcggac cacccggccc cttcttggca 480 catacgcacg ccggcctcca ggctcccggc cctctgcccg cccctgctgg tgacaaaggc 540 gatctgctgc tgcaagccgt ccagcaatcc tgcttggctg accacctgct gaccgctagc 600 tggggagccg accccgttcc caccaaggct cccggagaag gacaggaggg cctgcccctt 660 accggcgctt gcgcgggggg ccctggcttg cctgccggcg aactgtacgg ctgggccgtg 720 gagaccacgc cttcccccga gggcgtgtcc agcctggacg atgatgacct ggatctgtgc 780 ctggacagcg ccaacgacct gtacgatgac atcgagtgct ttttgatgga gctggagcag 840 cccgcc 846 < 210 > 158 < 211 > 1224 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : truncated Hu IRF 7 1 - 151 + 247 - 503 ; P 038 without epitope tag ; null mutation < 400 > 158 atggccctgg cccccgagag agccgcgccc agagtgctgt tcggcgaatg gctgctgggc 60 gagatcagca gcggctgcta tgagggcctg cagtggctcg acgaagccag gacgtgcttc 120 agagtcccct ggaagcactt cgccagaaag gatctgagcg aggctgacgc cagaatcttc 180 aaggcctggg cagttgcgcg tgggagatgg ccccccagct cgcggggcgg cggtcccccc 240 cctgaggccg agaccgccga aagagccgga tggaaaacca actttcgatg cgccctcaga 300 agcaccagac ggtttgtgat gctgagagat aacagcggcg accctgcaga cccccataaa 360 gtgtatgccc tgagcagaga gctgtgttgg cgagagggcc ccggaaccga ccaaaccgag 420 gccgaggccc ccgccgccgt acccccccct caaggccccc agcctgctgc tctgaccacg 480 ggagaagccg ccgctcctga gagcccccac caagccgagc cctatctgag ccctagcccc 540 agcgcctgca ccgccgtgca ggagccctca ccgggcgccc tagacgtgac catcatgtac 600 aaggggcgca cggtgctgca aaaggtggtg ggccacccca gctgcacctt cctgtacggc 660 ccccccgacc ctgccgtgag agccaccgac ccccagcaag tcgccttccc cagccccgcc 720 gagctgcccg accagaagca gctgaggtac accgaggagt tgctgagaca tgtggccccc 780 ggcttgcacc tcgagctgag aggcccgcag ctctgggcca gaagaatggg caagtgcaag 840 gtgtactggg aggtgggcgg cccccccggc agcgcgagcc caagcacccc ggcctgcctg 900 ctgcctagaa actgcgacac ccctatcttc gacttcagag tatttttcca ggagctggtc 960 gagttcaggg ccagacagcg tagaggcagc cccagataca ccatctacct tggattcggc 1020 caggacctga gcgccggcag acccaaagag aagtccctgg tactggtgaa gctagagccc 1080 tggctgtgta gggtgcatct ggaaggcacc caaagagagg gcgtaagctc gcttgacagc 1140 agcagcctca gcctgtgcct gagcagcgct aacagcttat acgacgacat cgagtgcttc 1200 ctgatggagc tggaacaacc cgcc 1224 < 210 > 159 < 211 > 1059 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : truncated Hu IRF 7 152 - 503 ; P 039 without epitope tag ; null mutation < 400 > 159 atgggcggcc ctcccgggcc tttcctggcc catacacacg ccggcctaca ggctcctggc 60 cctctgcccg ccccggccgg cgacaagggc gacctcctgc tgcaggccgt gcagcagtcc 120 tgtctggccg accacctgct gactgctagc tggggcgccg atcccgtgcc caccaaggcc 180 ccaggagagg ggcaagaggg cctgcctcta accggcgcat gcgcaggtgg accaggcctc 240 cccgccggcg agctgtatgg ttgggccgtg gagacaaccc ccagccccgg cccgcagcct 300 gctgcgctga ccacaggcga ggccgctgcc cctgagagcc cccaccaagc tgaaccctac 360 ctgagcccca gcccctctgc ctgcacagcg gtgcaggagc ccagtcccgg cgccttggac 420 gtgaccatca tgtataaggg caggactgtg ttacaaaagg tagtgggcca cccaagttgt 480 acctttctgt acgggccccc cgacccagcc gtgcgcgcca ccgaccccca gcaggtggcc 540 ttccccagcc ccgctgagtt gcccgatcag aaacaactcc ggtacaccga ggaattactt 600 agacatgtgg ctcccggcct gcatctggag cttagaggtc cacagttgtg ggccagaaga 660 atgggcaagt gcaaggttta ttgggaggtc ggaggccccc cgggcagcgc cagccccagc 720 acccccgcct gtcttctgcc cagaaactgc gacaccccaa tcttcgattt cagagtgttt 780 ttccaggaac tggtggagtt cagagcaagg caaagaagag gcagccctag atacaccatc 840 tacctgggct ttggccaaga cctgagcgcc ggcagaccca aggaaaaatc cctggtcctg 900 gtgaaactgg agccctggct gtgcagagtc cacctggagg gcacccagag agagggcgtg 960 agcagcctgg actcgagcag cctgtccctg tgtctgagca gcgcgaattc gctatatgac 1020 gacatcgaat gctttctgat ggagctggaa cagcccgcc 1059 < 210 > 160 < 211 > 1269 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer _ nt . STING ( V 155 M ) < 400 > 160 atgcctcaca gcagcctcca ccctagcatc ccttgcccta gaggccacgg cgcccagaag 60 gccgccctcg tgcttttaag cgcctgcttg gtgacccttt ggggcttggg cgagcctcca 120 gagcacacct tgagatattt ggtgctccac ctggccagcc ttcagctggg cttgttactc 180 aacggcgtgt gcagcctggc cgaggagctg agacacatcc acagcagata cagaggcagc 240 tactggagaa ccgtgagagc gtgtctgggc tgccctctga gaagaggcgc cttgcttctt 300 ctcagtatct acttctacta ctccctgcct aacgccgtgg gccctccttt cacctggatg 360 ctggcactgc tcggcctcag ccaggccctg aacatcttgt tgggcttgaa gggcctggcc 420 cctgccgaga tcagcgccgt gtgcgagaag ggcaacttca acatggccca cggattggct 480 tggagctact acatcggcta cctgagactg atcctgcctg agctgcaggc cagaatcaga 540 acctacaacc agcactacaa caacctgctg cgcggcgcag tgagccagag actgtatatt 600 ctgctgcctc tggactgcgg cgtgcctgac aacctgagca tggccgaccc taacatcaga 660 ttcctggaca agctgcctca gcagaccggc gaccacgccg gcatcaagga cagagtgtac 720 agcaacagca tctatgagct gctcgagaat ggccagagag ccggcacctg cgtgctggag 780 tacgccaccc ctctgcagac cctgttcgcc atgagccagt atagtcaagc tggcttcagc 840 agagaggaca gactggagca ggccaagctg ttctgcagaa ccctggagga cattctggct 900 gacgcccctg agagccagaa caactgccga ctgatcgcct accaggaacc agccgacgac 960 agcagcttca gtctttctca ggaggttctt cgccacttgc gccaggagga gaaggaggag 1020 gtgaccgtgg gcagcctgaa gacctccgca gtccctagca ccagcaccat gagtcaggag 1080 ccggagctat taatcagcgg catggagaag cctcttccac tccgaaccga cttcagcgcc 1140 accaacttca gcctgctgaa gcaggcaggt gacgttgagg agaatccggg acctatgacc 1200 gagtacaagc tggtggttgt gggcgccgac ggcgtgggca agagcgccct gaccatccag 1260 ctgatccag 1269 < 210 > 161 < 211 > 1269 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer _ ct . STING ( V 155 M ) < 400 > 161 atgaccgagt acaagctagt agtcgtgggc gccgacggcg tgggcaagag cgccctcacc 60 atccagctaa tccaggccac caacttcagc ttgctcaagc aggccggcga cgtggaggag 120 aacccaggcc ctatgcctca cagcagcctt caccctagca tcccttgccc tagaggccac 180 ggcgcccaga aggccgccct ggtgctgctg agcgcctgcc tggtgaccct gtggggcctg 240 ggcgagcctc ctgagcacac cctgagatat ctggtgcttc acctggccag tttacagctg 300 ggcctgcttc ttaacggcgt gtgcagcctg gccgaggagc tgagacacat ccacagcaga 360 tacagaggca gctactggag aaccgtgaga gcctgcctag gctgccctct gagaagaggc 420 gctctgttgc tactttccat ctacttctac tactccctgc ctaacgccgt gggccctcct 480 ttcacttgga tgctggcgtt gctgggtctg agccaggccc tgaacatcct tctcggtctg 540 aagggcctgg cccctgccga gatcagcgcc gtgtgcgaga agggcaactt caacatggcc 600 cacggactcg cctggagcta ctacatcggc tacctgagac tgatcctgcc tgagctgcag 660 gccagaatca gaacctacaa ccagcactac aacaacctgc tgcggggcgc cgtgagccag 720 agactgtata tacttcttcc tctggactgc ggcgtgcctg acaacctgag catggccgac 780 cctaacatca gattcctgga caagctgcct cagcagaccg gcgaccacgc cggcatcaag 840 gacagagtgt acagcaactc catttatgag ctgctcgaga atggccagag agccggcacc 900 tgcgtgctgg agtacgccac ccctctgcag accctgttcg ccatgagcca gtacagtcag 960 gctggattca gcagagagga cagactggag caggccaagc tgttctgcag gacactggag 1020 gacatactag cagacgcccc tgagagccag aacaactgca gactgattgc ctaccaggag 1080 cctgcggacg acagctcctt cagtctgagt caggaggtgt tgcggcactt acgccaagaa 1140 gagaaggagg aggtgaccgt gggcagcctg aagactagcg ctgtgcctag caccagcaca 1200 atgtcacagg agccggaatt gctaatcagc ggcatggaga agcctctccc attacgtacc 1260 gacttcagc 1269 < 210 > 162 < 211 > 1419 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer ^ 3 _ nt . STING ( V 155 M ) < 400 > 162 atgcctcaca gcagccttca ccctagcatc ccttgcccta gaggccacgg cgcccagaag 60 gccgccctag tgctccttag cgcctgcctc gtgaccctat ggggcttagg cgagcctcca 120 gagcacacct tgagatacct cgtcctccac ctggctagtc tacagctggg ccttctcctc 180 aacggcgtgt gcagcctggc cgaggagctg agacacatcc acagcagata cagaggcagc 240 tactggagaa ccgtgagagc gtgcctgggc tgccctctga gaagaggcgc actgctgtta 300 ctcagcatct acttctacta ctcactgcca aacgccgtgg gccctccttt cacctggatg 360 ctggccttgc tcggattgag ccaggccctg aacattttac tgggattgaa gggcctggcc 420 cctgccgaga tcagcgccgt gtgcgagaag ggcaacttca acatggccca cggcctagct 480 tggagctact acatcggcta cctgagactg atcctgcctg agctgcaggc cagaatcaga 540 acctacaacc agcactacaa caacctgctg cgtggagcgg tgagccagag actgtatatc 600 ctcctgcctc tggactgcgg agtgcctgac aacctgagca tggccgaccc taacatcaga 660 ttcctggaca agctgcctca gcagaccggc gaccacgccg gcatcaagga cagagtgtac 720 agcaactcaa tctacgagct gttggagaat ggccagagag ccggcacctg cgtgctggag 780 tacgccaccc ctctgcagac cctgttcgcc atgagccagt actctcaggc aggcttcagc 840 agagaggaca gactggagca ggccaagctg ttctgcagaa ccctggagga catcctggcg 900 gacgcccctg agagccagaa caactgccgg cttatcgcct accaggagcc agcagacgac 960 agcagcttct ctctctcaca agaggtactg cgccatcttc gccaggagga gaaggaggag 1020 gtgaccgtgg gcagcctgaa gacatccgcc gtacctagca ccagcaccat gtctcaggaa 1080 ccggaactgt tgatcagcgg catggagaag cctctgccac tgcgcaccga cttcagcgcc 1140 accaacttct ccctactgaa gcaagccggt gacgttgaag agaaccctgg ccctatgacc 1200 gagtacaagc tggtagtagt aggcgccgac ggcgtgggca agagcgccct gaccatccag 1260 ctgatccaga tgactgaata taagcttgtc gtcgtgggcg cagatggcgt tggtaagagc 1320 gcacttacaa ttcaactcat tcagatgacg gagtataagc tggtggtggt cggagctgac 1380 ggcgtaggca agagtgccct tactattcag ctaattcag 1419 < 210 > 163 < 211 > 1419 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer ^ 3 _ ct . STING ( V 155 M ) < 400 > 163 atgaccgagt acaagcttgt ggtggttggc gccgacggcg tgggcaagag cgccttaacc 60 atccagctta tccagatgac agagtataag ctagtggtgg tcggcgcaga cggagtggga 120 aagagtgcat taactattca actcatccaa atgaccgaat acaagctagt agttgtgggt 180 gcagatggcg tcggcaagtc tgcactgaca attcagctca tccaggccac caacttcagc 240 ctgctgaagc aggccggcga cgtggaggag aaccctggcc ctatgcctca cagcagcctg 300 caccctagca tcccttgccc tagaggccac ggcgcccaga aggccgccct ggtgctgctg 360 agcgcctgcc tggtgaccct gtggggcctg ggcgagcctc ctgagcacac cctgagatac 420 ctagttttgc acctggcttc tctgcagctg ggcctactgc tcaacggcgt gtgcagcctg 480 gccgaggagc tgagacacat ccacagcaga tacagaggca gctactggag aaccgtgaga 540 gcatgcttag gctgccctct gagaagaggc gctctgctcc tcttgtccat ctacttctac 600 tactcgctac ctaacgccgt gggccctcct ttcacctgga tgctggccct cttgggatta 660 agccaggccc tgaacatctt gctgggactg aagggcctgg cccctgccga gatcagcgcc 720 gtgtgcgaga agggcaactt caacatggcc cacggactcg cttggagcta ctacatcggc 780 tacctgagac tgatcctgcc tgagctgcag gccagaatca gaacctacaa ccagcactac 840 aacaacctgc tgcggggagc agtgagccag agactgtata ttctgctccc tctggactgc 900 ggcgtgcctg acaacctgag catggccgac cctaacatca gattcctgga caagctgcct 960 cagcagaccg gcgaccacgc cggcatcaag gacagagtgt acagcaacag catttacgag 1020 ctgctggaga acggccagag agccggcacc tgcgtgctgg agtacgccac ccctctgcag 1080 accctgttcg ccatgagcca gtactcccag gcaggattca gcagagagga cagactggag 1140 caggccaagc tgttctgccg tactcttgag gacatccttg cagacgcccc tgagagccag 1200 aacaactgcc ggttgattgc ctaccaggaa ccggcagacg acagctcatt ctccttgtct 1260 caggaggtcc ttagacacct gcggcaggag gagaaggagg aggtgaccgt gggcagcctg 1320 aagacatccg ccgtgcctag cacgtctacc atgtcccagg agccggaact gctaatcagc 1380 ggcatggaga agcctctgcc tctcaggacc gacttcagc 1419 < 210 > 164 < 211 > 380 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 284 K ) var ; no epitope tag < 400 > 164 Met Pro His Ser Ser Leu His Pro Ser Ile Pro Cys Pro Arg Gly His 1 5 10 15 Gly Ala Gln Lys Ala Ala Leu Val Leu Leu Ser Ala Cys Leu Val Thr 20 25 30 Leu Trp Gly Leu Gly Glu Pro Pro Glu His Thr Leu Arg Tyr Leu Val 35 40 45 Leu His Leu Ala Ser Leu Gln Leu Gly Leu Leu Leu Asn Gly Val Cys 50 55 60 Ser Leu Ala Glu Glu Leu Arg His Ile His Ser Arg Tyr Arg Gly Ser 65 70 75 80 Tyr Trp Arg Thr Val Arg Ala Cys Leu Gly Cys Pro Leu Arg Arg Gly 85 90 95 Ala Leu Leu Leu Leu Ser Ile Tyr Phe Tyr Tyr Ser Leu Pro Asn Ala 100 105 110 Val Gly Pro Pro Phe Thr Trp Met Leu Ala Leu Leu Gly Leu Ser Gln 115 120 125 Ala Leu Asn Ile Leu Leu Gly Leu Lys Gly Leu Ala Pro Ala Glu Ile 130 135 140 Ser Ala Val Cys Glu Lys Gly Asn Phe Asn Val Ala His Gly Leu Ala 145 150 155 160 Trp Ser Tyr Tyr Ile Gly Tyr Leu Arg Leu Ile Leu Pro Glu Leu Gln 165 170 175 Ala Arg Ile Arg Thr Tyr Asn Gln His Tyr Asn Asn Leu Leu Arg Gly 180 185 190 Ala Val Ser Gln Arg Leu Tyr Ile Leu Leu Pro Leu Asp Cys Gly Val 195 200 205 Pro Asp Asn Leu Ser Met Ala Asp Pro Asn Ile Arg Phe Leu Asp Lys 210 215 220 Leu Pro Gln Gln Thr Gly Asp His Ala Gly Ile Lys Asp Arg Val Tyr 225 230 235 240 Ser Asn Ser Ile Tyr Glu Leu Leu Glu Asn Gly Gln Arg Ala Gly Thr 245 250 255 Cys Val Leu Glu Tyr Ala Thr Pro Leu Gln Thr Leu Phe Ala Met Ser 260 265 270 Gln Tyr Ser Gln Ala Gly Phe Ser Arg Glu Asp Lys Leu Glu Gln Ala 275 280 285 Lys Leu Phe Cys Arg Thr Leu Glu Asp Ile Leu Ala Asp Ala Pro Glu 290 295 300 Ser Gln Asn Asn Cys Arg Leu Ile Ala Tyr Gln Glu Pro Ala Asp Asp 305 310 315 320 Ser Ser Phe Ser Leu Ser Gln Glu Val Leu Arg His Leu Arg Gln Glu 325 330 335 Glu Lys Glu Glu Val Thr Val Gly Ser Leu Lys Thr Ser Ala Val Pro 340 345 350 Ser Thr Ser Thr Met Ser Gln Glu Pro Glu Leu Leu Ile Ser Gly Met 355 360 365 Glu Lys Pro Leu Pro Leu Arg Thr Asp Phe Ser Thr 370 375 380 < 210 > 165 < 211 > 1140 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 284 K ) var ; no epitope tag < 400 > 165 atgccccata gcagcctgca ccccagcatc ccctgcccca gaggccacgg cgcccagaag 60 gccgccctgg tcctgctgag cgcatgcctg gtcaccctgt ggggcctggg cgagcccccc 120 gagcacaccc tgagatacct ggtgctgcac ctcgccagcc tgcagctggg cctgctgctg 180 aacggcgtgt gcagcctggc cgaggagctg agacacatcc acagcagata tagaggcagc 240 tactggagaa ccgtgagagc ttgcctcggc tgccccctga gaagaggcgc cctgctgctg 300 ctgagcatct acttttacta cagcctgccc aacgctgtgg gccccccttt cacgtggatg 360 ctcgccctgc tgggactgag ccaggccctg aacatcctgc tgggccttaa gggcctagcc 420 cccgccgaga tcagcgccgt gtgcgagaag ggcaacttca atgtggccca cggcctggcc 480 tggagctact acatcggcta cctgagactg atcctgcccg agctgcaggc cagaatcaga 540 acctacaatc agcactacaa caacctgctg agaggcgccg tgagccagag actgtacatc 600 ctgctgcccc tggactgcgg cgtgcccgac aacctcagca tggccgaccc caacatcaga 660 ttcctggaca agctgcccca gcagaccggc gaccacgccg gcatcaagga tcgcgtgtac 720 agcaacagca tctacgagct gctggaaaac ggccagagag ccggaacctg cgtgctggag 780 tacgccacac ccctgcagac cctgttcgcc atgagccagt acagccaggc cggcttcagc 840 agagaggaca agctggagca ggccaagctg ttctgcagaa ccctggagga tatcctcgcc 900 gacgcccccg agagccagaa caactgcagg ctgatcgcgt accaggagcc cgctgacgac 960 agcagcttta gcctgagcca ggaggtgctg agacatctgc gtcaagagga aaaggaggag 1020 gtgaccgtgg gctccctgaa gaccagcgcc gtgcccagca ccagcaccat gagccaggag 1080 cccgagctgc tgatcagcgg catggagaag ccactgcccc tcagaaccga cttcagcacc 1140 < 210 > 166 < 211 > 186 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 186 ) < 223 > Human KRAS sp / P 01116 [ 1 - 186 ] < 400 > 166 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Gly Val Gly Lys 1 5 10 15 Ser Ala Leu Thr Ile Gln Leu Ile Gln Asn His Phe Val Asp Glu Tyr 20 25 30 Asp Pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln Val Val Ile Asp Gly 35 40 45 Glu Thr Cys Leu Leu Asp Ile Leu Asp Thr Ala Gly Gln Glu Glu Tyr 50 55 60 Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu Cys 65 70 75 80 Val Phe Ala Ile Asn Asn Thr Lys Ser Phe Glu Asp Ile His His Tyr 85 90 95 Arg Glu Gln Ile Lys Arg Val Lys Asp Ser Glu Asp Val Pro Met Val 100 105 110 Leu Val Gly Asn Lys Cys Asp Leu Pro Ser Arg Thr Val Asp Thr Lys 115 120 125 Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly Ile Pro Phe Ile Glu Thr 130 135 140 Ser Ala Lys Thr Arg Gln Arg Val Glu Asp Ala Phe Tyr Thr Leu Val 145 150 155 160 Arg Glu Ile Arg Gln Tyr Arg Leu Lys Lys Ile Ser Lys Glu Glu Lys 165 170 175 Thr Pro Gly Cys Val Lys Ile Lys Lys Cys 180 185 < 210 > 167 < 211 > 570 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic < 220 > < 221 > misc _ feature < 223 > 5 ' 7 MeGpppG 2 ' Ome - OH 3 '; Where : A , C G & U = AMP , CMP , GMP & N 1 - psi - UMP , respectively ; Me = methyl ; p = inorganic phosphate ( KRAS concatemer mRNA sequence ; CX - 012908 ) < 400 > 167 ggaaauaaga gagaaaagaa gaguaagaag aaauauaaga gccaccauga ccgaguacaa 60 gcucgugguc gucggcgccg acgggguagg caaguccgcu cugaccauuc agcucaucca 120 gaugacggag uacaaacucg ugguaguggg agccgugggu gugggcaaga gcgcgcucac 180 cauccaacuc auccaaauga ccgaauauaa acucgucgug gugggagccg gcgacguggg 240 aaagagcgcc cuuaccaucc aguuaaucca gaugacagaa uacaagcugg ugguggucgg 300 ugccugcggc guggguaagu ccgcccugac aauccagcug auccagugau aauaggcugg 360 agccucggug gccaugcuuc uugccccuug ggccuccccc cagccccucc uccccuuccu 420 gcacccguac ccccgugguc uuugaauaaa gucugagugg gcggcaaaaa aaaaaaaaaa 480 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 540 aaaaaaaaaa aaaaaaaaaa aaaaaucuag 570 < 210 > 168 < 211 > 1429 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic < 220 > < 221 > misc _ feature < 223 > 5 ' 7 MeGpppG 2 ' Ome - OH 3 '; Where : A , C G & U = AMP , CMP , GMP & N 1 - psi - UMP , respectively ; Me = methyl ; p = inorganic phosphate ; underline = miR - 122 binding site ( STING mRNA sequence ; CX - 012871 ) < 400 > 168 ggaaauaaga gagaaaagaa gaguaagaag aaauauaaga gccaccaugc cccacaguag 60 ccuccacccc agcauccccu gccccagagg ccacggcgca cagaaggccg cccuggugcu 120 gcugagcgcc ugucugguga cccugugggg ucugggcgag ccccccgagc acacccugcg 180 guaccucgug cugcaucugg ccagccugca gcugggccug cugcugaacg gcgugugcag 240 ccuggccgaa gagcugagac acauccacag cagauacaga ggcuccuacu ggagaaccgu 300 cagagccugc cucggcuguc cccugagaag aggcgcccug cugcuccuga gcaucuacuu 360 cuacuacagc cugcccaacg ccgugggccc ccccuucacc uggaugcugg cccugcuggg 420 ccugagccag gcccugaaca uccugcuggg ccugaagggc uuggcccccg ccgagaucuc 480 cgccgugugc gagaagggca acuucaacau ggcccauggc cuugccuggu ccuacuacau 540 cggcuaccug agacugaucc ugcccgagcu gcaggccaga aucagaaccu acaaccagca 600 cuacaacaac cugcugagag gcgccgugag ccaaagacug uacauccugc ugccccugga 660 cugcggcgug cccgacaacc uuagcauggc cgaccccaac aucagauucc uggacaagcu 720 gccccagcag accggcgacc acgccggcau caaggacaga guguacagca acagcaucua 780 cgagcugcug gagaacggcc agagagccgg caccugcgug cuggaguacg ccaccccccu 840 gcagacccug uucgccauga gccaguacag ccaggccggc uucagcagag aggacagacu 900 ggagcaagcc aagcuguucu gcagaacccu ggaggacauc cuggcggacg cccccgagag 960 ccaaaacaac ugcagacuga ucgccuacca ggagcccgcc gacgacagca gcuucagccu 1020 gagccaggaa gugcugagac accugagaca ggaagagaag gaggagguga ccgugggaag 1080 ccugaagacc agcgccgugc ccagcaccag caccaugagc caggagcccg agcugcugau 1140 cagcggcaug gagaagcccc ugccccugag aaccgacuuc agcugauaau aggcuggagc 1200 cucgguggcc uagcuucuug ccccuugggc cuccccccag ccccuccucc ccuuccugca 1260 cccguacccc ccaaacacca uugucacacu ccaguggucu uugaauaaag ucugaguggg 1320 cggcaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1380 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaucuag 1429 < 210 > 169 < 211 > 300 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D G 12 V G 13 D G 12 C ) 100 mer 4 MUT nt . seq < 400 > 169 augaccgagu acaagcucgu ggucgucggc gccgacgggg uaggcaaguc cgcucugacc 60 auucagcuca uccagaugac ggaguacaaa cucgugguag ugggagccgu gggugugggc 120 aagagcgcgc ucaccaucca acucauccaa augaccgaau auaaacucgu cgugguggga 180 gccggcgacg ugggaaagag cgcccuuacc auccaguuaa uccagaugac agaauacaag 240 cugguggugg ucggugccug cggcgugggu aaguccgccc ugacaaucca gcugauccag 300 < 210 > 170 < 211 > 1137 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : huSTING ( V 155 M ) ; no epitope tag ; nucleotide sequence < 400 > 170 augccccaca guagccucca ccccagcauc cccugcccca gaggccacgg cgcacagaag 60 gccgcccugg ugcugcugag cgccugucug gugacccugu ggggucuggg cgagcccccc 120 gagcacaccc ugcgguaccu cgugcugcau cuggccagcc ugcagcuggg ccugcugcug 180 aacggcgugu gcagccuggc cgaagagcug agacacaucc acagcagaua cagaggcucc 240 uacuggagaa ccgucagagc cugccucggc uguccccuga gaagaggcgc ccugcugcuc 300 cugagcaucu acuucuacua cagccugccc aacgccgugg gcccccccuu caccuggaug 360 cuggcccugc ugggccugag ccaggcccug aacauccugc ugggccugaa gggcuuggcc 420 cccgccgaga ucuccgccgu gugcgagaag ggcaacuuca acauggccca uggccuugcc 480 ugguccuacu acaucggcua ccugagacug auccugcccg agcugcaggc cagaaucaga 540 accuacaacc agcacuacaa caaccugcug agaggcgccg ugagccaaag acuguacauc 600 cugcugcccc uggacugcgg cgugcccgac aaccuuagca uggccgaccc caacaucaga 660 uuccuggaca agcugcccca gcagaccggc gaccacgccg gcaucaagga cagaguguac 720 agcaacagca ucuacgagcu gcuggagaac ggccagagag ccggcaccug cgugcuggag 780 uacgccaccc cccugcagac ccuguucgcc augagccagu acagccaggc cggcuucagc 840 agagaggaca gacuggagca agccaagcug uucugcagaa cccuggagga cauccuggcg 900 gacgcccccg agagccaaaa caacugcaga cugaucgccu accaggagcc cgccgacgac 960 agcagcuuca gccugagcca ggaagugcug agacaccuga gacaggaaga gaaggaggag 1020 gugaccgugg gaagccugaa gaccagcgcc gugcccagca ccagcaccau gagccaggag 1080 cccgagcugc ugaucagcgg cauggagaag ccccugcccc ugagaaccga cuucagc 1137 < 210 > 171 < 211 > 85 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : mir - 122 < 400 > 171 ccuuagcaga gcuguggagu gugacaaugg uguuuguguc uaaacuauca aacgccauua 60 ucacacuaaa uagcuacugc uaggc 85 < 210 > 172 < 211 > 22 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : mir - 122 - 3 p _ < 400 > 172 aacgccauua ucacacuaaa ua 22 < 210 > 173 < 211 > 22 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : mir - 122 - 3 p binding site < 400 > 173 uauuuagugu gauaauggcg uu 22 < 210 > 174 < 211 > 22 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : mir - 122 - 5 p < 400 > 174 uggaguguga caaugguguu ug 22 < 210 > 175 < 211 > 22 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : mir - 122 - 5 p binding site < 400 > 175 caaacaccau ugucacacuc ca 22 < 210 > 176 < 211 > 46 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : 5 UTR < 400 > 176 ggaaauaaga gagaaaagaa gaguaagaag aaauauaaga gccacc 46 < 210 > 177 < 211 > 12 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : GC - Rich RNA Element < 400 > 177 ccgccgccgc cg 12 < 210 > 178 < 211 > 15 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : GC - Rich RNA Element < 400 > 178 ccgccgccgc cgccg 15 < 210 > 179 < 211 > 10 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : V 1 - GC - Rich RNA Element < 400 > 179 ccccggcgcc 10 < 210 > 180 < 211 > 41 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : 5 UTR < 400 > 180 gggaaataag agagaaaaga agagtaagaa gaaatataag a 41 < 210 > 181 < 211 > 57 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : V 1 - UTR < 400 > 181 gggaaataag agagaaaaga agagtaagaa gaaatataag accccggcgc cgccacc 57 < 210 > 182 < 211 > 54 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : V 2 - UTR < 400 > 182 gggaaataag agagaaaaga agagtaagaa gaaatataag accccggcgc cacc 54 < 210 > 183 < 211 > 45 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 C 15 mer ^ 3 < 400 > 183 Met Lys Leu Val Val Val Gly Ala Cys Gly Val Gly Lys Ser Ala Met 1 5 10 15 Lys Leu Val Val Val Gly Ala Cys Gly Val Gly Lys Ser Ala Met Lys 20 25 30 Leu Val Val Val Gly Ala Cys Gly Val Gly Lys Ser Ala 35 40 45 < 210 > 184 < 211 > 225 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 C 25 mer ^ 3 nucleotide sequence < 400 > 184 atgaccgagt acaagctcgt ggttgttggc gcctgcggcg tgggcaagag cgccctcacc 60 atccagctca tccagatgac agagtataag ttagtcgttg tcggagcttg cggagttgga 120 aagtcggcgc tcaccattca actcatacaa atgacagaat ataagttagt ggtggtgggt 180 gcgtgtggcg ttggcaagag tgcgcttact atccagctca ttcag 225 < 210 > 185 < 211 > 92 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : 5 UTR < 400 > 185 ucaagcuuuu ggacccucgu acagaagcua auacgacuca cuauagggaa auaagagaga 60 aaagaagagu aagaagaaau auaagagcca cc 92 < 210 > 186 < 211 > 119 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : 3 UTR < 400 > 186 ugauaauagg cuggagccuc gguggccaug cuucuugccc cuugggccuc cccccagccc 60 cuccuccccu uccugcaccc guacccccgu ggucuuugaa uaaagucuga gugggcggc 119 < 210 > 187 < 211 > 164 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : 3 UTR with mi - 122 and mi - 142 . 3 p sites < 400 > 187 ugauaauagg cuggagccuc gguggccaug cuucuugccc cuugggccca aacaccauug 60 ucacacucca uccccccagc cccuccuccc cuuccuccau aaaguaggaa acacuacaug 120 cacccguacc cccguggucu uugaauaaag ucugaguggg cggc 164 < 210 > 188 < 211 > 66 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Nucleotide sequence encoding 2 A peptide < 400 > 188 ggaagcggag cuacuaacuu cagccugcug aagcaggcug gagacgugga ggagaacccu 60 ggaccu 66 < 210 > 189 < 211 > 108 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Nucleotide sequence encoding 2 A peptide < 400 > 189 uccggacuca gauccgggga ucucaaaauu gucgcuccug ucaaacaaac ucuuaacuuu 60 gauuuacuca aacuggcugg ggauguagaa agcaauccag guccacuc 108 < 210 > 190 < 211 > 75 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 D 25 mer nucleotide sequence < 400 > 190 augaccgagu acaagcuggu gguggugggc gccgacggcg ugggcaagag cgcccugacc 60 auccagcuga uccag 75 < 210 > 191 < 211 > 75 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 V 25 mer nucleotide sequence < 400 > 191 augaccgagu acaagcuggu gguggugggc gccgugggcg ugggcaagag cgcccugacc 60 auccagcuga uccag 75 < 210 > 192 < 211 > 75 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 13 D 25 mer nucleotide sequence < 400 > 192 augaccgagu acaagcuggu gguggugggc gccggcgacg ugggcaagag cgcccugacc 60 auccagcuga uccag 75 < 210 > 193 < 211 > 225 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 D 25 mer ^ 3 nucleotide sequence < 400 > 193 augaccgagu acaaguuagu gguugugggc gccgacggcg ugggcaagag cgcccucacc 60 auccagcuua uccagaugac ggaauauaag uuaguaguag ugggagccga cggugucggc 120 aaguccgcuu ugaccauuca acuuauucag augacagagu auaagcuggu cguuguaggc 180 gcagacggcg uuggaaaguc ggcacugacg auccaguuga uccag 225 < 210 > 194 < 211 > 225 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 V 25 mer ^ 3 nucleotide sequence < 400 > 194 augaccgagu acaagcucgu cguggugggc gccgugggcg ugggcaagag cgcccuaacc 60 auccaguuga uccagaugac cgaauauaag cucgugguag ucggagcggu gggcguuggc 120 aagucagcgc uaacaauaca acuaauccaa augaccgaau acaagcuagu uguagucggu 180 gccgucggcg uuggaaaguc agcccuuaca auucagcuca uucag 225 < 210 > 195 < 211 > 225 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 13 D 25 mer ^ 3 nucleotide sequence < 400 > 195 augaccgagu acaagcucgu agugguuggc gccggcgacg ugggcaagag cgcccuaacc 60 auccagcuca uccagaugac agaauauaag cuugugguug ugggagcagg agacguggga 120 aagagugcgu ugacgauuca acucauacag augaccgaau acaaguuggu gguggucggc 180 gcaggugacg uugguaaguc ugcacuaacu auacaacuga uccag 225 < 210 > 196 < 211 > 75 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 C 25 mer nucleotide sequence < 400 > 196 augaccgagu acaagcuggu gguggugggc gccugcggcg ugggcaagag cgcccugacc 60 auccagcuga uccag 75 < 210 > 197 < 211 > 225 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS G 12 C 25 mer ^ 3 nucleotide sequence < 400 > 197 augaccgagu acaagcucgu gguuguuggc gccugcggcg ugggcaagag cgcccucacc 60 auccagcuca uccagaugac agaguauaag uuagucguug ucggagcuug cggaguugga 120 aagucggcgc ucaccauuca acucauacaa augacagaau auaaguuagu gguggugggu 180 gcguguggcg uuggcaagag ugcgcuuacu auccagcuca uucag 225 < 210 > 198 < 211 > 75 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS WT 25 mer nucleotide sequence < 400 > 198 augaccgagu acaagcuggu gguggugggc gccggcggcg ugggcaagag cgcccugacc 60 auccagcuga uccag 75 < 210 > 199 < 211 > 47 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : 5 UTR sequence ; no promoter < 400 > 199 gggaaauaag agagaaaaga agaguaagaa gaaauauaag agccacc 47 < 210 > 200 < 211 > 225 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D G 12 V G 13 D ) 75 mer 3 MUT nt . seq < 400 > 200 augaccgagu acaagcucgu uguagucggc gccgacggcg ugggcaagag cgccuugacc 60 auccaguuga uccagaugac cgaauauaag uugguggugg uaggcgcagu gggaguuggc 120 aagucagcac ucacaauuca gcucauucaa augacagaau acaaguuagu cguuguagga 180 gcaggcgacg ucggcaagag ugccuuaacc auucaacuaa uccag 225 < 210 > 201 < 211 > 1137 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 284 T ) ; no epitope tag ; nucleotide sequence < 400 > 201 augccucaca gcagccugca cccuagcauc ccuugcccua gaggccacgg cgcccagaag 60 gccgcccugg ugcugcugag cgccugccug gugacccugu ggggccuggg cgagccuccu 120 gagcacaccc ugagauaccu ggugcugcac cuggccagcc ugcagcuggg ccugcugcug 180 aacggcgugu gcagccuggc cgaggagcug agacacaucc acagcagaua cagaggcagc 240 uacuggagaa ccgugagagc cugccugggc ugcccucuga gaagaggcgc ccugcugcug 300 cugagcaucu acuucuacua cagccugccu aacgccgugg gcccuccuuu caccuggaug 360 cuggcccugc ugggccugag ccaggcccug aacauccugc ugggccugaa gggccuggcc 420 ccugccgaga ucagcgccgu gugcgagaag ggcaacuuca acguggccca cggccuggcc 480 uggagcuacu acaucggcua ccugagacug auccugccug agcugcaggc cagaaucaga 540 accuacaacc agcacuacaa caaccugcug agaggcgccg ugagccagag acuguacauc 600 cugcugccuc uggacugcgg cgugccugac aaccugagca uggccgaccc uaacaucaga 660 uuccuggaca agcugccuca gcagaccggc gaccacgccg gcaucaagga cagaguguac 720 agcaacagca ucuacgagcu gcuggagaac ggccagagag ccggcaccug cgugcuggag 780 uacgccaccc cucugcagac ccuguucgcc augagccagu acagccaggc cggcuucagc 840 agagaggaca cccuggagca ggccaagcug uucugcagaa cccuggagga cauccuggcc 900 gacgccccug agagccagaa caacugcaga cugaucgccu accaggagcc ugccgacgac 960 agcagcuuca gccugagcca ggaggugcug agacaccuga gacaggagga gaaggaggag 1020 gugaccgugg gcagccugaa gaccagcgcc gugccuagca ccagcaccau gagccaggag 1080 ccugagcugc ugaucagcgg cauggagaag ccucugccuc ugagaaccga cuucagc 1137 < 210 > 202 < 211 > 1137 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : hu STING ( R 284 M ) ; no epitope tag ; nucleotide sequence < 400 > 202 augccccaca gcagccugca ccccuccauc cccuguccca gaggccacgg cgcccagaag 60 gccgcccugg ugcugcugag cgccugccug gugaccuuau ggggccuggg cgagcccccc 120 gagcacaccc ugagauaccu gguccugcac cuggccagcc uccagcuggg ccugcugcuc 180 aacggcgugu guagccuggc cgaggagcug agacacaucc acagcagaua cagaggcagc 240 uacuggagaa ccgugagagc cugccugggu ugcccacuga gaagaggagc ucugcugcug 300 cugagcaucu acuucuacua cucgcugccc aacgcugugg gcccccccuu caccuggaug 360 cuggcccugc ugggucugag ccaggcccug aacauccucc ugggccugaa gggccuggcc 420 cccgccgaga uaagcgccgu uugcgagaag ggcaacuuca acguggccca uggccuggcc 480 uggagcuacu acaucggcua cuuacgccug auccugcccg agcugcaggc cagaaucaga 540 accuacaacc agcauuacaa caaccugcug agaggcgccg ugagccagag acuguauauc 600 cugcugcccc uggacugcgg cgugcccgac aaccugagca uggccgaccc caacaucaga 660 uuccuggaca agcuccccca gcagaccggc gaccacgccg gaaucaaaga cagaguguau 720 agcaacagca ucuacgagcu gcuggagaac ggccagagag ccggcaccug cguacuggag 780 uacgccaccc ccuugcagac ccuguuugcc augagccagu acagccaggc cggcuucagc 840 agagaggaca ugcuggagca ggccaagcug uucugcagaa cccuggagga cauccuggcc 900 gacgcccccg agagccagaa caacugcaga cugaucgccu accaagagcc cgccgacgac 960 agcagcuuca gcuuaagcca ggaggugcug agacaucuga gacaggagga gaaggaggag 1020 gugaccgugg gcagccucaa gaccagcgcu gugcccucua ccagcaccau gagccaggag 1080 cccgagcugc ugaucagcgg cauggagaag ccccugcccc ugagaacaga cuucagc 1137 < 210 > 203 < 211 > 1137 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 284 K ) ; no epitope tag ; nucleotide sequence < 400 > 203 augccccaua gcagccugca ccccagcauc cccugcccca gaggccacgg cgcccagaag 60 gccgcccugg uccugcugag cgcaugccug gucacccugu ggggccuggg cgagcccccc 120 gagcacaccc ugagauaccu ggugcugcac cucgccagcc ugcagcuggg ccugcugcug 180 aacggcgugu gcagccuggc cgaggagcug agacacaucc acagcagaua uagaggcagc 240 uacuggagaa ccgugagagc uugccucggc ugcccccuga gaagaggcgc ccugcugcug 300 cugagcaucu acuuuuacua cagccugccc aacgcugugg gccccccuuu cacguggaug 360 cucgcccugc ugggacugag ccaggcccug aacauccugc ugggccuuaa gggccuagcc 420 cccgccgaga ucagcgccgu gugcgagaag ggcaacuuca auguggccca cggccuggcc 480 uggagcuacu acaucggcua ccugagacug auccugcccg agcugcaggc cagaaucaga 540 accuacaauc agcacuacaa caaccugcug agaggcgccg ugagccagag acuguacauc 600 cugcugcccc uggacugcgg cgugcccgac aaccucagca uggccgaccc caacaucaga 660 uuccuggaca agcugcccca gcagaccggc gaccacgccg gcaucaagga ucgcguguac 720 agcaacagca ucuacgagcu gcuggaaaac ggccagagag ccggaaccug cgugcuggag 780 uacgccacac cccugcagac ccuguucgcc augagccagu acagccaggc cggcuucagc 840 agagaggaca agcuggagca ggccaagcug uucugcagaa cccuggagga uauccucgcc 900 gacgcccccg agagccagaa caacugcagg cugaucgcgu accaggagcc cgcugacgac 960 agcagcuuua gccugagcca ggaggugcug agacaucugc gucaagagga aaaggaggag 1020 gugaccgugg gcucccugaa gaccagcgcc gugcccagca ccagcaccau gagccaggag 1080 cccgagcugc ugaucagcgg cauggagaag ccacugcccc ucagaaccga cuucagc 1137 < 210 > 204 < 211 > 1137 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( N 154 S ) ; no epitope tag ; nucleotide sequence < 400 > 204 augccucaca gcagccugca cccuagcauc ccuugcccua gaggccacgg cgcccagaag 60 gccgcccugg ugcugcugag cgccugccug gugacccugu ggggccuggg cgagccuccu 120 gagcacaccc ugagauaccu ggugcugcac cuggccagcc ugcagcuggg ccugcugcug 180 aacggcgugu gcagccuggc cgaggagcug agacacaucc acagcagaua cagaggcagc 240 uacuggagaa ccgugagagc cugccugggc ugcccucuga gaagaggcgc ccugcugcug 300 cugagcaucu acuucuacua cagccugccu aacgccgugg gcccuccuuu caccuggaug 360 cuggcccugc ugggccugag ccaggcccug aacauccugc ugggccugaa gggccuggcc 420 ccugccgaga ucagcgccgu gugcgagaag ggcaacuuca gcguggccca cggccuggcc 480 uggagcuacu acaucggcua ccugagacug auccugccug agcugcaggc cagaaucaga 540 accuacaacc agcacuacaa caaccugcug agaggcgccg ugagccagag acuguacauc 600 cugcugccuc uggacugcgg cgugccugac aaccugagca uggccgaccc uaacaucaga 660 uuccuggaca agcugccuca gcagaccggc gaccacgccg gcaucaagga cagaguguac 720 agcaacagca ucuacgagcu gcuggagaac ggccagagag ccggcaccug cgugcuggag 780 uacgccaccc cucugcagac ccuguucgcc augagccagu acagccaggc cggcuucagc 840 agagaggaca gacuggagca ggccaagcug uucugcagaa cccuggagga cauccuggcc 900 gacgccccug agagccagaa caacugcaga cugaucgccu accaggagcc ugccgacgac 960 agcagcuuca gccugagcca ggaggugcug agacaccuga gacaggagga gaaggaggag 1020 gugaccgugg gcagccugaa gaccagcgcc gugccuagca ccagcaccau gagccaggag 1080 ccugagcugc ugaucagcgg cauggagaag ccucugccuc ugagaaccga cuucagc 1137 < 210 > 205 < 211 > 1137 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( V 147 L ) ; no epitope tag ; nucleotide sequence < 400 > 205 augccucaca gcagccugca cccuagcauc ccuugcccua gaggccacgg cgcccagaag 60 gccgcccugg ugcugcugag cgccugccug gugacccugu ggggccuggg cgagccuccu 120 gagcacaccc ugagauaccu ggugcugcac cuggccagcc ugcagcuggg ccugcugcug 180 aacggcgugu gcagccuggc cgaggagcug agacacaucc acagcagaua cagaggcagc 240 uacuggagaa ccgugagagc cugccugggc ugcccucuga gaagaggcgc ccugcugcug 300 cugagcaucu acuucuacua cagccugccu aacgccgugg gcccuccuuu caccuggaug 360 cuggcccugc ugggccugag ccaggcccug aacauccugc ugggccugaa gggccuggcc 420 ccugccgaga ucagcgcccu gugcgagaag ggcaacuuca acguggccca cggccuggcc 480 uggagcuacu acaucggcua ccugagacug auccugccug agcugcaggc cagaaucaga 540 accuacaacc agcacuacaa caaccugcug agaggcgccg ugagccagag acuguacauc 600 cugcugccuc uggacugcgg cgugccugac aaccugagca uggccgaccc uaacaucaga 660 uuccuggaca agcugccuca gcagaccggc gaccacgccg gcaucaagga cagaguguac 720 agcaacagca ucuacgagcu gcuggagaac ggccagagag ccggcaccug cgugcuggag 780 uacgccaccc cucugcagac ccuguucgcc augagccagu acagccaggc cggcuucagc 840 agagaggaca gacuggagca ggccaagcug uucugcagaa cccuggagga cauccuggcc 900 gacgccccug agagccagaa caacugcaga cugaucgccu accaggagcc ugccgacgac 960 agcagcuuca gccugagcca ggaggugcug agacaccuga gacaggagga gaaggaggag 1020 gugaccgugg gcagccugaa gaccagcgcc gugccuagca ccagcaccau gagccaggag 1080 ccugagcugc ugaucagcgg cauggagaag ccucugccuc ugagaaccga cuucagc 1137 < 210 > 206 < 211 > 1137 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( E 315 Q ) ; no epitope tag ; nucleotide sequence < 400 > 206 augccucaca gcagccugca cccuagcauc ccuugcccua gaggccacgg cgcccagaag 60 gccgcccugg ugcugcugag cgccugccug gugacccugu ggggccuggg cgagccuccu 120 gagcacaccc ugagauaccu ggugcugcac cuggccagcc ugcagcuggg ccugcugcug 180 aacggcgugu gcagccuggc cgaggagcug agacacaucc acagcagaua cagaggcagc 240 uacuggagaa ccgugagagc cugccugggc ugcccucuga gaagaggcgc ccugcugcug 300 cugagcaucu acuucuacua cagccugccu aacgccgugg gcccuccuuu caccuggaug 360 cuggcccugc ugggccugag ccaggcccug aacauccugc ugggccugaa gggccuggcc 420 ccugccgaga ucagcgccgu gugcgagaag ggcaacuuca acguggccca cggccuggcc 480 uggagcuacu acaucggcua ccugagacug auccugccug agcugcaggc cagaaucaga 540 accuacaacc agcacuacaa caaccugcug agaggcgccg ugagccagag acuguacauc 600 cugcugccuc uggacugcgg cgugccugac aaccugagca uggccgaccc uaacaucaga 660 uuccuggaca agcugccuca gcagaccggc gaccacgccg gcaucaagga cagaguguac 720 agcaacagca ucuacgagcu gcuggagaac ggccagagag ccggcaccug cgugcuggag 780 uacgccaccc cucugcagac ccuguucgcc augagccagu acagccaggc cggcuucagc 840 agagaggaca gacuggagca ggccaagcug uucugcagaa cccuggagga cauccuggcc 900 gacgccccug agagccagaa caacugcaga cugaucgccu accagcagcc ugccgacgac 960 agcagcuuca gccugagcca ggaggugcug agacaccuga gacaggagga gaaggaggag 1020 gugaccgugg gcagccugaa gaccagcgcc gugccuagca ccagcaccau gagccaggag 1080 ccugagcugc ugaucagcgg cauggagaag ccucugccuc ugagaaccga cuucagc 1137 < 210 > 207 < 211 > 1137 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 375 A ) ; no epitope tag ; nucleotide sequence < 400 > 207 augccucaca gcagccugca cccuagcauc ccuugcccua gaggccacgg cgcccagaag 60 gccgcccugg ugcugcugag cgccugccug gugacccugu ggggccuggg cgagccuccu 120 gagcacaccc ugagauaccu ggugcugcac cuggccagcc ugcagcuggg ccugcugcug 180 aacggcgugu gcagccuggc cgaggagcug agacacaucc acagcagaua cagaggcagc 240 uacuggagaa ccgugagagc cugccugggc ugcccucuga gaagaggcgc ccugcugcug 300 cugagcaucu acuucuacua cagccugccu aacgccgugg gcccuccuuu caccuggaug 360 cuggcccugc ugggccugag ccaggcccug aacauccugc ugggccugaa gggccuggcc 420 ccugccgaga ucagcgccgu gugcgagaag ggcaacuuca acguggccca cggccuggcc 480 uggagcuacu acaucggcua ccugagacug auccugccug agcugcaggc cagaaucaga 540 accuacaacc agcacuacaa caaccugcug agaggcgccg ugagccagag acuguacauc 600 cugcugccuc uggacugcgg cgugccugac aaccugagca uggccgaccc uaacaucaga 660 uuccuggaca agcugccuca gcagaccggc gaccacgccg gcaucaagga cagaguguac 720 agcaacagca ucuacgagcu gcuggagaac ggccagagag ccggcaccug cgugcuggag 780 uacgccaccc cucugcagac ccuguucgcc augagccagu acagccaggc cggcuucagc 840 agagaggaca gacuggagca ggccaagcug uucugcagaa cccuggagga cauccuggcc 900 gacgccccug agagccagaa caacugcaga cugaucgccu accaggagcc ugccgacgac 960 agcagcuuca gccugagcca ggaggugcug agacaccuga gacaggagga gaaggaggag 1020 gugaccgugg gcagccugaa gaccagcgcc gugccuagca ccagcaccau gagccaggag 1080 ccugagcugc ugaucagcgg cauggagaag ccucugccuc uggccaccga cuucagc 1137 < 210 > 208 < 211 > 1137 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( V 147 L / N 154 S / V 155 M ) ; no epitope tag ; nucleotide sequence < 400 > 208 augccucaca gcagccugca cccuagcauc ccuugcccua gaggccacgg cgcccagaag 60 gccgcccugg ugcugcugag cgccugccug gugacccugu ggggccuggg cgagccuccu 120 gagcacaccc ugagauaccu ggugcugcac cuggccagcc ugcagcuggg ccugcugcug 180 aacggcgugu gcagccuggc cgaggagcug agacacaucc acagcagaua cagaggcagc 240 uacuggagaa ccgugagagc cugccugggc ugcccucuga gaagaggcgc ccugcugcug 300 cugagcaucu acuucuacua cagccugccu aacgccgugg gcccuccuuu caccuggaug 360 cuggcccugc ugggccugag ccaggcccug aacauccugc ugggccugaa gggccuggcc 420 ccugccgaga ucagcgcccu gugcgagaag ggcaacuuca gcauggccca cggccuggcc 480 uggagcuacu acaucggcua ccugagacug auccugccug agcugcaggc cagaaucaga 540 accuacaacc agcacuacaa caaccugcug agaggcgccg ugagccagag acuguacauc 600 cugcugccuc uggacugcgg cgugccugac aaccugagca uggccgaccc uaacaucaga 660 uuccuggaca agcugccuca gcagaccggc gaccacgccg gcaucaagga cagaguguac 720 agcaacagca ucuacgagcu gcuggagaac ggccagagag ccggcaccug cgugcuggag 780 uacgccaccc cucugcagac ccuguucgcc augagccagu acagccaggc cggcuucagc 840 agagaggaca gacuggagca ggccaagcug uucugcagaa cccuggagga cauccuggcc 900 gacgccccug agagccagaa caacugcaga cugaucgccu accaggagcc ugccgacgac 960 agcagcuuca gccugagcca ggaggugcug agacaccuga gacaggagga gaaggaggag 1020 gugaccgugg gcagccugaa gaccagcgcc gugccuagca ccagcaccau gagccaggag 1080 ccugagcugc ugaucagcgg cauggagaag ccucugccuc ugagaaccga cuucagc 1137 < 210 > 209 < 211 > 1137 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 284 M / V 147 L / N 154 S / V 155 M ) ; no epitope tag ; nucleotide sequence < 400 > 209 augccucaca gcagccugca cccuagcauc ccuugcccua gaggccacgg cgcccagaag 60 gccgcccugg ugcugcugag cgccugccug gugacccugu ggggccuggg cgagccuccu 120 gagcacaccc ugagauaccu ggugcugcac cuggccagcc ugcagcuggg ccugcugcug 180 aacggcgugu gcagccuggc cgaggagcug agacacaucc acagcagaua cagaggcagc 240 uacuggagaa ccgugagagc cugccugggc ugcccucuga gaagaggcgc ccugcugcug 300 cugagcaucu acuucuacua cagccugccu aacgccgugg gcccuccuuu caccuggaug 360 cuggcccugc ugggccugag ccaggcccug aacauccugc ugggccugaa gggccuggcc 420 ccugccgaga ucagcgcccu gugcgagaag ggcaacuuca gcauggccca cggccuggcc 480 uggagcuacu acaucggcua ccugagacug auccugccug agcugcaggc cagaaucaga 540 accuacaacc agcacuacaa caaccugcug agaggcgccg ugagccagag acuguacauc 600 cugcugccuc uggacugcgg cgugccugac aaccugagca uggccgaccc uaacaucaga 660 uuccuggaca agcugccuca gcagaccggc gaccacgccg gcaucaagga cagaguguac 720 agcaacagca ucuacgagcu gcuggagaac ggccagagag ccggcaccug cgugcuggag 780 uacgccaccc cucugcagac ccuguucgcc augagccagu acagccaggc cggcuucagc 840 agagaggaca ugcuggagca ggccaagcug uucugcagaa cccuggagga cauccuggcc 900 gacgccccug agagccagaa caacugcaga cugaucgccu accaggagcc ugccgacgac 960 agcagcuuca gccugagcca ggaggugcug agacaccuga gacaggagga gaaggaggag 1020 gugaccgugg gcagccugaa gaccagcgcc gugccuagca ccagcaccau gagccaggag 1080 ccugagcugc ugaucagcgg cauggagaag ccucugccuc ugagaaccga cuucagc 1137 < 210 > 210 < 211 > 141 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : 3 UTR used in STING V 155 M construct , containing miR 122 binding site < 400 > 210 ugauaauagg cuggagccuc gguggccuag cuucuugccc cuugggccuc cccccagccc 60 cuccuccccu uccugcaccc guacccccca aacaccauug ucacacucca guggucuuug 120 aauaaagucu gagugggcgg c 141 < 210 > 211 < 211 > 1257 < 212 > RNA < 213 > Mus musculus < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1257 ) < 223 > super mouse IRF 3 S 396 D ; no epitope tag < 400 > 211 auggagaccc ccaagccuag aauccugccc uggcugguga gccagcugga ccugggccag 60 cuggagggcg uagccuggcu ggacgagagc agaaccagau ucagaauccc cuggaagcac 120 ggccugagac aagacgccca gauggccgac uucggcaucu uccaggccug ggccgaggcc 180 agcggcgccu acaccccugg caaggauaag cccgauguga gcaccuggaa gagaaacuuc 240 agaagcgccc ugaacagaaa ggaggugcug agacuggccg ccgacaauag caaggacccc 300 uacgaccccc acaaggugua cgaguucguu acccccggcg ccagggacuu cgugcaccug 360 ggcgccagcc ccgacaccaa cggcaagagc agccugcccc acagccagga gaaccugccc 420 aagcuguucg auggccugau ccugggcccc cugaaggacg agggcagcag cgaccuggcc 480 aucgugagcg acccuagcca gcagcugccc ucccccaacg ugaacaacuu ccugaacccc 540 gccccccagg agaacccccu gaagcaacug cuggccgagg agcaguggga guucgaggug 600 accgccuucu acagaggcag acagguguuc cagcagaccc uguucugccc cggcggccug 660 agacugguag gcagcaccgc ugacaugacc cugcccuggc agcccgugac ccugcccgac 720 cccgaaggcu uucugaccga caagcuggug aaggaguacg ucggccaagu gcugaagggc 780 cugggcaacg gccuggcccu guggcaggcc ggccagugcc ugugggccca gagacucggc 840 cacagccacg ccuucugggc ccugggcgag gaacuccugc ccgauagcgg cagaggcccc 900 gacggcgagg ugcacaagga caaggacggc gccguguucg accugcgccc cuucguggcc 960 gaccugaucg ccuucaugga gggcagcggc cacagcccca gauauacccu gugguucugc 1020 augggcgaga uguggcccca ggaccagccc ugggugaaga gacuggugau ggugaaggug 1080 gugcccaccu gccugaaaga gcugcuggag auggccagag agggcggcgc cagcucccug 1140 aaaaccgugg accugcacau ugacaacagc cagcccauca gccugaccag cgaccaguac 1200 aaggccuacc ugcaggaccu gguggaggac auggacuucc aggccaccgg caacauc 1257 < 210 > 212 < 211 > 1281 < 212 > RNA < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1281 ) < 223 > super human IRF 3 S 396 D ; no epitope tag < 400 > 212 augggcaccc ccaagcccag aauccugccc uggcugguga gccagcugga ccugggccag 60 cuggagggag uggccugggu gaacaagagc agaaccagau ucagaauccc cuggaagcac 120 ggccucagac aggacgccca gcaggaggac uucggcauuu uucaggcuug ggccgaggcc 180 accggcgccu acgugcccgg cagagacaag cccgaccugc ccaccuggaa aagaaacuuc 240 agaagcgccu ugaauagaaa ggagggccug agacuggccg aggacagaag caaggacccc 300 cacgacccuc acaagaucua cgaguucgug aauagcggcg ugggcgacuu uagccagccc 360 gacaccagcc ccgacaccaa cggcggcggc agcaccagcg acacgcagga ggacauccug 420 gaugaacugc ugggcaacau ggugcuggcc ccccugcccg aucccggccc cccuucgcuu 480 gccguggccc ccgagcccug cccccagccc cugagaagcc ccucucugga uaaccccacc 540 cccuucccca accugggccc cagcgagaau ccacugaaga gacuucuggu ccccggcgag 600 gagugggagu ucgaggugac cgccuucuac agaggcagac agguguucca gcagaccauc 660 agcugccccg aaggccugag auuagugggc agcgaagugg gcgacaggac ccugcccggg 720 uggcccguga cccugcccga ucccggcaug agccugaccg acagaggugu gaugagcuac 780 gugagacacg ugcugagcug ccugggcggc ggccuggcac uguggagagc cggccagugg 840 cugugggccc agagacuggg ccacugccac accuacuggg ccgugagcga ggagcugcug 900 cccaacagcg gccacggccc cgacggcgag gugcccaagg acaaggaagg gggcguguuc 960 gaccugggcc ccuucaucgu agaccugauc accuuuaccg agggcagcgg caggagcccc 1020 agauacgccc ugugguucug cgugggcgaa agcuggcccc aggaccagcc cuggaccaag 1080 agacugguga uggugaaggu agugcccacc ugccugagag ccuuagugga gauggccaga 1140 gugggcgggg ccagcagccu ggagaacacc guggaucuuc acaucgacaa cagccacccc 1200 cugagccuga ccagcgacca guacaaggcc uaccugcagg accuggugga gggcauggac 1260 uuccagggcc ccggcgagac c 1281 < 210 > 213 < 211 > 1509 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Wild - type Hu IRF 7 isoform A ; P 037 without epitope tag < 400 > 213 auggcgcugg cccccgaaag agccgccccc agaguccucu ucggcgaaug gcuccuuggc 60 gaaauuucgu cgggcugcua cgagggcuua caauggcugg augaggcgag aaccuguuuc 120 agggugcccu ggaaacacuu cgccagaaag gaucuaagcg aagcagaugc uagaauuuuu 180 aaggcuuggg ccguggccag gggaagaugg ccccccucga gcagaggcgg cggcccuccc 240 cccgaggcag aaacggccga gagagccgga uggaaaacca auuucagaug cgcccugaga 300 ucuacaagaa gauucgugau gcuuagagac aacagcggag aucccgccga uccccauaag 360 guguaugccc ugucccggga gcugugcugg agggaagggc cuggcacuga ccagaccgaa 420 gccgaagccc ccgcggccgu gccgccgccc caaggaggcc caccaggccc uuuccucgcu 480 cacacccacg ccggucugca agccccggga ccucuaccug ccccugccgg cgauaaaggc 540 gaccuguugc ugcaggccgu ccaacagagc ugccuggccg aucaucugcu cacagccagc 600 uggggcgcug accccguccc aacaaaggcc cccggugagg gccaagaagg ccugccucug 660 accggcgccu gugccggcgg cccuggccug ccugcuggcg agcuguacgg augggcuguc 720 gaaaccacuc ccucccccgg cccccaaccu gcggcccuga caaccggcga ggcagccgca 780 cccgaaagcc cccaccaggc cgaacccuac cucaguccca gccccuccgc cugcaccgcu 840 gugcaggagc ccagccccgg ugcucuggac guaacaauca uguacaaagg cagaaccgug 900 cuucagaagg ugguuggaca ccccuccugu acuuuucucu acggcccccc cgacccugcc 960 gugagagcua ccgacccgca acagguggcc uuucccucgc ccgccgaacu gcccgaucaa 1020 aaacagcuga gauacaccga ggagcugcug agacacgugg cgccgggcuu acaccuagag 1080 uugagaggcc cccaacucug ggccagacgc augggcaagu guaaggugua cugggagguc 1140 gggggcccuc ccggcucugc cagccccagc accccugcuu gucucuugcc cagaaacugu 1200 gauaccccca ucuucgacuu ccguguauuu uuccaggaac uggucgaguu uagagccaga 1260 cagagacgag gcagccccag auauacaauc uaccucggcu ucggccagga ccugagugcc 1320 ggcagaccua aggagaaguc gcugguccua gugaaguuag agcccuggcu auguagagug 1380 caccuggagg gcacccagag agaaggagug agcagccugg acagcagcag ccugagucug 1440 ugccugagcu ccgccaacuc gcuguaugau gacaucgagu guuuccucau ggagcuggag 1500 cagcccgcc 1509 < 210 > 214 < 211 > 1509 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active Hu IRF 7 S 477 D / S 479 D ; P 033 without epitope tag < 400 > 214 auggcccuug ccccugagcg ggccgccccc agaguguuau ucggcgagug gcugcugggc 60 gagaucagca gcggcugcua cgagggacug caguggcugg acgaggcuag aaccugcuuc 120 agagugcccu ggaagcauuu cgccagaaaa gaccugagcg aggcugaugc uagaaucuuc 180 aaagccuggg cuguggcccg aggaagaugg ccccccagca gcagaggagg cggcccuccu 240 cccgaggccg aaaccgcaga gcgugcuggc uggaaaacca acuuuaggug ugcccugagg 300 agcaccagaa gauucguuau gcucagagac aacagcgggg accccgccga cccgcacaag 360 guguacgccu uaaguaggga gcugugcugg agagagggac cggggaccga ccaaaccgag 420 gcugaggcgc ccgccgccgu uccaccuccc cagggugguc ccccagggcc cuuucuggca 480 cacacccacg ccggauuaca ggcgccaggg cccuuacccg cccccgccgg agacaaaggc 540 gaccuccugc ugcaagccgu gcaacaaagc ugccuggccg aucacuuacu aaccgcuagc 600 uggggcgccg auccuguucc caccaaggcc cccggugaag ggcaagaagg acugcccuua 660 accggcgccu gugccggagg cccuggucug ccagccggcg agcuguacgg uugggcuguc 720 gaaacaacac ccaguccggg cccacagccu gccgcucuga ccaccggcga agccgccgcc 780 cccgagagcc cacaccaggc ugaacccuac cugagcccca gccccagcgc cugcaccgcu 840 gugcaggagc cuagccccgg cgcucuugau gugacaauaa uguacaaggg caggaccgug 900 cugcaaaagg ucgugggcca uccgucgugu accuuucugu acggcccucc agaccccgcg 960 guuagagcca ccgaccccca gcaagucgcc uuccccuccc ccgccgaacu gcccgaccaa 1020 aagcagcugc gguacacaga agaacuacuu agacacgugg cccccggucu gcacuuggag 1080 cugagaggcc cccagcucug ggccagaaga augggcaagu gcaaagugua cugggaggug 1140 ggcggcccac ccggcucagc uucgcccucc acacccgcau gccugcugcc cagaaauugc 1200 gacacgccca ucuucgauuu uagaguguuc uuucaggagu ugguggaguu cagagccaga 1260 caaagacgcg gcagccccag auacaccauu uaccucggcu ucggccagga ccucagcgcu 1320 ggcagaccca aggagaagag ucugguccuc gugaagcugg agcccuggcu gugcagagug 1380 caccuggagg gcacccagcg ugaaggcgug agcagccugg auucaagcga ccuggaccua 1440 ugccuaagca gcgcuaacuc acuguacgac gauaucgaau gcuuccugau ggaacuggag 1500 cagccugcc 1509 < 210 > 215 < 211 > 1509 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active Hu IRF 7 S 475 D / S 477 D / L 480 D ; P 034 without epitope tag < 400 > 215 auggcccugg cacccgagag ggccgccccc agggugcucu ucggcgagug guuacuaggc 60 gaaauuagca gcggcugcua ugaaggccuu caguggcugg acgaggccag aaccugcuuu 120 agaguucccu ggaagcacuu cgcccggaaa gaucucucug aagccgacgc cagaauauuc 180 aaggccuggg cugucgccag gggcaggugg ccacccucca gccgaggugg cggcccuccc 240 ccugaggcug agacugcgga aagggcgggc uggaagacca auuucagaug cgcucugaga 300 agcaccagac guuuugugau gcuaagagac aauagcggcg aucccgccga cccccauaag 360 guauacgcac ugagccgaga gcucuguugg agagaaggcc ccggcaccga ccagaccgag 420 gcugaagccc cugcagccgu gccccccccu caaggcgggc cccccggccc cuuccuggcc 480 cauacccaug caggguuaca agcacccggg cccuugcccg ccccagcggg agacaagggc 540 gaccucuuac ugcaggccgu gcaacaaagu ugucuggcgg accaccugcu gaccgcauca 600 uggggcgcgg auccugugcc caccaaggca cccggcgaag gccaggaggg ccugcccuug 660 accggcgccu gcgcuggcgg acccggccua ccugcuggcg aacuguaugg cugggccgua 720 gagacgacuc ccagcccugg cccacaaccc gcggcuuuga ccaccggcga agccgccgcc 780 cccgagucuc cgcaccaggc cgagccuuac cucagcccaa gcccuagcgc cugcaccgcc 840 gugcaagaac cuagccccgg agcccuggau gugacaauca uguacaaggg uagaaccgua 900 cugcaaaagg ugguggguca ucccagcugc accuuucuuu acggcccacc cgacccugcc 960 gugcgagcca cagacccaca acaggucgcc uucccaagcc ccgccgaacu gcccgaucag 1020 aaacagcuga gauauacaga ggagcuucug cggcacguag cucccggccu acaucucgag 1080 cugaggggcc cacaacugug ggccagacgc augggcaaau gcaaggucua cugggaagug 1140 ggaggccccc ccggcagcgc aucucccagc acgcccgcgu gccugcugcc uagaaauugc 1200 gacaccccca ucuuugacuu ccggguauuc uuucaggagc ugguagaguu cagagccagg 1260 cagcggaggg gcucccccag auacacaauc uaccugggcu ucggacagga ccuguccgcc 1320 ggccgcccca aggaaaagag ccuggugcug gugaagcugg agcccuggcu guguagggua 1380 caccucgaag gcacccagag agaaggagug agcucgcuug augacagcga ucugucggau 1440 ugccuuagca gcgccaacag ccuguaugau gauaucgagu gcuuccuuau ggaacuggag 1500 cagcccgcc 1509 < 210 > 216 < 211 > 1509 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active Hu IRF 7 S 475 D / S 476 D / S 477 D / S 479 D / S 483 D / S 487 D ; P 035 without epitope tag < 400 > 216 auggcccuag cccccgaaag agcagcuccc agagugcugu ucggcgaaug gcugcuuggc 60 gagaucagca gcggcugcua cgaaggccug caguggcugg acgaagcccg caccuguuuc 120 agagugcccu ggaagcacuu cgcuagaaag gauuugagcg aggcugaugc uagaaucuuu 180 aaggcuuggg cuguggcaag aggcagaugg ccgccuagua gcagaggggg cggaccuccc 240 cccgaggcug agaccgcuga gagagcaggg uggaaaacca acuucagaug cgcgcugaga 300 agcacccgaa gauucgugau gcuacgugac aauagcggcg accccgccga cccccacaaa 360 guguacgccc ugucccgaga acuuugcugg agagagggac ccggcaccga ucaaacagag 420 gcugaggccc cggccgcugu acccccgccc caaggaggcc ccccaggccc cuuucuggcu 480 cauacacaug ccggccugca ggcacccggg ccccucccgg cuccugccgg cgacaagggc 540 gaucuccuuc uccaggccgu gcagcagagc ugccuggccg aucaccugcu gaccgccucg 600 uggggcgccg accccgugcc caccaaagcc ccgggugaag gccaagaggg gcucccuuua 660 accggagcau gcgccggagg ccccggccug ccagccggcg aguuauaugg cugggcugug 720 gagaccacac ccucccccgg cccucaaccc gcugcccuga ccaccgguga ggccgccgcc 780 cccgagagcc cacaccaggc cgaacccuac cugagcccua gcccuagcgc cugcaccgcc 840 gugcaagaac ccagccccgg agcccuggau gugaccauua uguacaaggg ccggacagug 900 cugcaaaagg uugugggaca cccgagcugc accuuucugu acgguccgcc ugaccccgcc 960 gugagagcca cggacccgca gcagguggcc uuccccucac ccgcggagcu gcccgaccaa 1020 aagcaacuca gauacacaga agaacuauug cgucacgucg cgcccggccu gcaucuggag 1080 cugagaggcc cccagcucug ggccagaagg augggcaaau gcaaggugua cugggaggug 1140 ggaggccccc ccggcagcgc cagccccagc acucccgcgu gccugcugcc cagaaauugc 1200 gacacuccca ucuucgauuu caggguguuc uuccaggagc ugguggaguu cagagccagg 1260 cagagaaggg guagccccag auacacaauc uaucuaggcu uuggacaaga ucugagcgcc 1320 ggccggccua aggaaaaaag ccuggugcug guaaagcugg agccguggcu uuguagagug 1380 caccuggagg ggacgcagcg agagggcgug agcagcuuag acgacgauga cuuggaucug 1440 ugucucgaca gcgccaacga cuuguacgac gacaucgagu gcuuccugau ggaacuggag 1500 cagcccgcc 1509 < 210 > 217 < 211 > 846 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active truncated Hu IRF 7 1 - 246 + 468 - 503 ; P 032 without epitope tag < 400 > 217 auggcccugg cccccgagag agccgccccc agagugcucu ucggcgagug gcugcugggc 60 gagauaagca gcggcugcua cgaaggucug caguggcuag acgaggccag aaccugcuuu 120 agagugcccu ggaagcacuu cgcucgaaag gaccuguccg aggccgaugc uagaauuuuu 180 aaggcuuggg ccgucgcuag gggaagaugg cccccuagca guagaggcgg cggccccccu 240 cccgaagccg agacggccga gagggccggc uggaaaacca auuucagaug cgcccugagg 300 agcacccgca gguucguaau gcugcgagac aauagcggcg auccugcgga uccucacaag 360 guuuacgccu ugaguagaga acugugcugg cgggagggcc ccggaaccga ccagacggag 420 gcagaggcac ccgcugccgu gccccccccu caaggaggac ccccuggacc cuuucuggcc 480 cacacccacg cuggucugca ggccccaggc ccacugcccg ccccagcggg cgauaagggu 540 gaccugcucc uacaggcggu gcaacagagc ugucuggccg accaccuguu gaccgccagc 600 uggggggccg acccggugcc caccaaagcu cccggagagg gccaagaagg ccucccacua 660 acuggcgccu gcgccggggg cccgggauua cccgccggcg agcuguaugg cugggccgug 720 gagaccacgc ccagccccga gggcgugucg ucccuggaca gcagcagccu gagccugugc 780 cugagcuccg ccaacagccu guaugacgac aucgagugcu uccugaugga gcuggaacaa 840 cccgcc 846 < 210 > 218 < 211 > 846 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : constitutively active truncated Hu IRF 7 1 - 246 + 468 - 503 plus S 475 D / S 476 D / S 477 D / S 479 D / S 483 D / S 487 D ; P 036 without epitope tag < 400 > 218 auggcacugg cgccugaaag agccgcuccg cgugugcucu ucggcgagug gcugcugggc 60 gagaucagcu ccggcugcua cgagggucua caguggcugg acgaggccag aaccuguuuu 120 agagugcccu ggaagcacuu cgcgagaaag gaccugagcg aggccgacgc cagaaucuuc 180 aaagccuggg caguggcuag gggcagaugg ccucccagca gccggggcgg cggcccaccc 240 cccgaggccg aaaccgccga aagagcuggc uggaagacca acuucagaug cgcccugaga 300 agcaccagaa gauuugucau gcugagagau aauucaggag accccgccga cccucacaag 360 guguacgccc uguccagaga gcuguguugg agagagggcc ccggaaccga ccagaccgag 420 gccgaggcuc cagcugccgu gccacccccc caaggcggac cacccggccc cuucuuggca 480 cauacgcacg ccggccucca ggcucccggc ccucugcccg ccccugcugg ugacaaaggc 540 gaucugcugc ugcaagccgu ccagcaaucc ugcuuggcug accaccugcu gaccgcuagc 600 uggggagccg accccguucc caccaaggcu cccggagaag gacaggaggg ccugccccuu 660 accggcgcuu gcgcgggggg cccuggcuug ccugccggcg aacuguacgg cugggccgug 720 gagaccacgc cuucccccga gggcgugucc agccuggacg augaugaccu ggaucugugc 780 cuggacagcg ccaacgaccu guacgaugac aucgagugcu uuuugaugga gcuggagcag 840 cccgcc 846 < 210 > 219 < 211 > 1224 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : truncated Hu IRF 7 1 - 151 + 247 - 503 ; P 038 without epitope tag ; null mutation < 400 > 219 auggcccugg cccccgagag agccgcgccc agagugcugu ucggcgaaug gcugcugggc 60 gagaucagca gcggcugcua ugagggccug caguggcucg acgaagccag gacgugcuuc 120 agaguccccu ggaagcacuu cgccagaaag gaucugagcg aggcugacgc cagaaucuuc 180 aaggccuggg caguugcgcg ugggagaugg ccccccagcu cgcggggcgg cggucccccc 240 ccugaggccg agaccgccga aagagccgga uggaaaacca acuuucgaug cgcccucaga 300 agcaccagac gguuugugau gcugagagau aacagcggcg acccugcaga cccccauaaa 360 guguaugccc ugagcagaga gcuguguugg cgagagggcc ccggaaccga ccaaaccgag 420 gccgaggccc ccgccgccgu accccccccu caaggccccc agccugcugc ucugaccacg 480 ggagaagccg ccgcuccuga gagcccccac caagccgagc ccuaucugag cccuagcccc 540 agcgccugca ccgccgugca ggagcccuca ccgggcgccc uagacgugac caucauguac 600 aaggggcgca cggugcugca aaagguggug ggccacccca gcugcaccuu ccuguacggc 660 ccccccgacc cugccgugag agccaccgac ccccagcaag ucgccuuccc cagccccgcc 720 gagcugcccg accagaagca gcugagguac accgaggagu ugcugagaca uguggccccc 780 ggcuugcacc ucgagcugag aggcccgcag cucugggcca gaagaauggg caagugcaag 840 guguacuggg aggugggcgg cccccccggc agcgcgagcc caagcacccc ggccugccug 900 cugccuagaa acugcgacac cccuaucuuc gacuucagag uauuuuucca ggagcugguc 960 gaguucaggg ccagacagcg uagaggcagc cccagauaca ccaucuaccu uggauucggc 1020 caggaccuga gcgccggcag acccaaagag aagucccugg uacuggugaa gcuagagccc 1080 uggcugugua gggugcaucu ggaaggcacc caaagagagg gcguaagcuc gcuugacagc 1140 agcagccuca gccugugccu gagcagcgcu aacagcuuau acgacgacau cgagugcuuc 1200 cugauggagc uggaacaacc cgcc 1224 < 210 > 220 < 211 > 1059 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : truncated Hu IRF 7 152 - 503 ; P 039 without epitope tag ; null mutation < 400 > 220 augggcggcc cucccgggcc uuuccuggcc cauacacacg ccggccuaca ggcuccuggc 60 ccucugcccg ccccggccgg cgacaagggc gaccuccugc ugcaggccgu gcagcagucc 120 ugucuggccg accaccugcu gacugcuagc uggggcgccg aucccgugcc caccaaggcc 180 ccaggagagg ggcaagaggg ccugccucua accggcgcau gcgcaggugg accaggccuc 240 cccgccggcg agcuguaugg uugggccgug gagacaaccc ccagccccgg cccgcagccu 300 gcugcgcuga ccacaggcga ggccgcugcc ccugagagcc cccaccaagc ugaacccuac 360 cugagcccca gccccucugc cugcacagcg gugcaggagc ccagucccgg cgccuuggac 420 gugaccauca uguauaaggg caggacugug uuacaaaagg uagugggcca cccaaguugu 480 accuuucugu acgggccccc cgacccagcc gugcgcgcca ccgaccccca gcagguggcc 540 uuccccagcc ccgcugaguu gcccgaucag aaacaacucc gguacaccga ggaauuacuu 600 agacaugugg cucccggccu gcaucuggag cuuagagguc cacaguugug ggccagaaga 660 augggcaagu gcaagguuua uugggagguc ggaggccccc cgggcagcgc cagccccagc 720 acccccgccu gucuucugcc cagaaacugc gacaccccaa ucuucgauuu cagaguguuu 780 uuccaggaac ugguggaguu cagagcaagg caaagaagag gcagcccuag auacaccauc 840 uaccugggcu uuggccaaga ccugagcgcc ggcagaccca aggaaaaauc ccugguccug 900 gugaaacugg agcccuggcu gugcagaguc caccuggagg gcacccagag agagggcgug 960 agcagccugg acucgagcag ccugucccug ugucugagca gcgcgaauuc gcuauaugac 1020 gacaucgaau gcuuucugau ggagcuggaa cagcccgcc 1059 < 210 > 221 < 211 > 1269 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer _ nt . STING ( V 155 M ) < 400 > 221 augccucaca gcagccucca cccuagcauc ccuugcccua gaggccacgg cgcccagaag 60 gccgcccucg ugcuuuuaag cgccugcuug gugacccuuu ggggcuuggg cgagccucca 120 gagcacaccu ugagauauuu ggugcuccac cuggccagcc uucagcuggg cuuguuacuc 180 aacggcgugu gcagccuggc cgaggagcug agacacaucc acagcagaua cagaggcagc 240 uacuggagaa ccgugagagc gugucugggc ugcccucuga gaagaggcgc cuugcuucuu 300 cucaguaucu acuucuacua cucccugccu aacgccgugg gcccuccuuu caccuggaug 360 cuggcacugc ucggccucag ccaggcccug aacaucuugu ugggcuugaa gggccuggcc 420 ccugccgaga ucagcgccgu gugcgagaag ggcaacuuca acauggccca cggauuggcu 480 uggagcuacu acaucggcua ccugagacug auccugccug agcugcaggc cagaaucaga 540 accuacaacc agcacuacaa caaccugcug cgcggcgcag ugagccagag acuguauauu 600 cugcugccuc uggacugcgg cgugccugac aaccugagca uggccgaccc uaacaucaga 660 uuccuggaca agcugccuca gcagaccggc gaccacgccg gcaucaagga cagaguguac 720 agcaacagca ucuaugagcu gcucgagaau ggccagagag ccggcaccug cgugcuggag 780 uacgccaccc cucugcagac ccuguucgcc augagccagu auagucaagc uggcuucagc 840 agagaggaca gacuggagca ggccaagcug uucugcagaa cccuggagga cauucuggcu 900 gacgccccug agagccagaa caacugccga cugaucgccu accaggaacc agccgacgac 960 agcagcuuca gucuuucuca ggagguucuu cgccacuugc gccaggagga gaaggaggag 1020 gugaccgugg gcagccugaa gaccuccgca gucccuagca ccagcaccau gagucaggag 1080 ccggagcuau uaaucagcgg cauggagaag ccucuuccac uccgaaccga cuucagcgcc 1140 accaacuuca gccugcugaa gcaggcaggu gacguugagg agaauccggg accuaugacc 1200 gaguacaagc uggugguugu gggcgccgac ggcgugggca agagcgcccu gaccauccag 1260 cugauccag 1269 < 210 > 222 < 211 > 1269 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer _ ct . STING ( V 155 M ) < 400 > 222 augaccgagu acaagcuagu agucgugggc gccgacggcg ugggcaagag cgcccucacc 60 auccagcuaa uccaggccac caacuucagc uugcucaagc aggccggcga cguggaggag 120 aacccaggcc cuaugccuca cagcagccuu cacccuagca ucccuugccc uagaggccac 180 ggcgcccaga aggccgcccu ggugcugcug agcgccugcc uggugacccu guggggccug 240 ggcgagccuc cugagcacac ccugagauau cuggugcuuc accuggccag uuuacagcug 300 ggccugcuuc uuaacggcgu gugcagccug gccgaggagc ugagacacau ccacagcaga 360 uacagaggca gcuacuggag aaccgugaga gccugccuag gcugcccucu gagaagaggc 420 gcucuguugc uacuuuccau cuacuucuac uacucccugc cuaacgccgu gggcccuccu 480 uucacuugga ugcuggcguu gcugggucug agccaggccc ugaacauccu ucucggucug 540 aagggccugg ccccugccga gaucagcgcc gugugcgaga agggcaacuu caacauggcc 600 cacggacucg ccuggagcua cuacaucggc uaccugagac ugauccugcc ugagcugcag 660 gccagaauca gaaccuacaa ccagcacuac aacaaccugc ugcggggcgc cgugagccag 720 agacuguaua uacuucuucc ucuggacugc ggcgugccug acaaccugag cauggccgac 780 ccuaacauca gauuccugga caagcugccu cagcagaccg gcgaccacgc cggcaucaag 840 gacagagugu acagcaacuc cauuuaugag cugcucgaga auggccagag agccggcacc 900 ugcgugcugg aguacgccac cccucugcag acccuguucg ccaugagcca guacagucag 960 gcuggauuca gcagagagga cagacuggag caggccaagc uguucugcag gacacuggag 1020 gacauacuag cagacgcccc ugagagccag aacaacugca gacugauugc cuaccaggag 1080 ccugcggacg acagcuccuu cagucugagu caggaggugu ugcggcacuu acgccaagaa 1140 gagaaggagg aggugaccgu gggcagccug aagacuagcg cugugccuag caccagcaca 1200 augucacagg agccggaauu gcuaaucagc ggcauggaga agccucuccc auuacguacc 1260 gacuucagc 1269 < 210 > 223 < 211 > 1419 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer ^ 3 _ nt . STING ( V 155 M ) < 400 > 223 augccucaca gcagccuuca cccuagcauc ccuugcccua gaggccacgg cgcccagaag 60 gccgcccuag ugcuccuuag cgccugccuc gugacccuau ggggcuuagg cgagccucca 120 gagcacaccu ugagauaccu cguccuccac cuggcuaguc uacagcuggg ccuucuccuc 180 aacggcgugu gcagccuggc cgaggagcug agacacaucc acagcagaua cagaggcagc 240 uacuggagaa ccgugagagc gugccugggc ugcccucuga gaagaggcgc acugcuguua 300 cucagcaucu acuucuacua cucacugcca aacgccgugg gcccuccuuu caccuggaug 360 cuggccuugc ucggauugag ccaggcccug aacauuuuac ugggauugaa gggccuggcc 420 ccugccgaga ucagcgccgu gugcgagaag ggcaacuuca acauggccca cggccuagcu 480 uggagcuacu acaucggcua ccugagacug auccugccug agcugcaggc cagaaucaga 540 accuacaacc agcacuacaa caaccugcug cguggagcgg ugagccagag acuguauauc 600 cuccugccuc uggacugcgg agugccugac aaccugagca uggccgaccc uaacaucaga 660 uuccuggaca agcugccuca gcagaccggc gaccacgccg gcaucaagga cagaguguac 720 agcaacucaa ucuacgagcu guuggagaau ggccagagag ccggcaccug cgugcuggag 780 uacgccaccc cucugcagac ccuguucgcc augagccagu acucucaggc aggcuucagc 840 agagaggaca gacuggagca ggccaagcug uucugcagaa cccuggagga cauccuggcg 900 gacgccccug agagccagaa caacugccgg cuuaucgccu accaggagcc agcagacgac 960 agcagcuucu cucucucaca agagguacug cgccaucuuc gccaggagga gaaggaggag 1020 gugaccgugg gcagccugaa gacauccgcc guaccuagca ccagcaccau gucucaggaa 1080 ccggaacugu ugaucagcgg cauggagaag ccucugccac ugcgcaccga cuucagcgcc 1140 accaacuucu cccuacugaa gcaagccggu gacguugaag agaacccugg cccuaugacc 1200 gaguacaagc ugguaguagu aggcgccgac ggcgugggca agagcgcccu gaccauccag 1260 cugauccaga ugacugaaua uaagcuuguc gucgugggcg cagauggcgu ugguaagagc 1320 gcacuuacaa uucaacucau ucagaugacg gaguauaagc uggugguggu cggagcugac 1380 ggcguaggca agagugcccu uacuauucag cuaauucag 1419 < 210 > 224 < 211 > 1419 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : KRAS ( G 12 D ) 25 mer ^ 3 _ ct . STING ( V 155 M ) < 400 > 224 augaccgagu acaagcuugu ggugguuggc gccgacggcg ugggcaagag cgccuuaacc 60 auccagcuua uccagaugac agaguauaag cuaguggugg ucggcgcaga cggaguggga 120 aagagugcau uaacuauuca acucauccaa augaccgaau acaagcuagu aguugugggu 180 gcagauggcg ucggcaaguc ugcacugaca auucagcuca uccaggccac caacuucagc 240 cugcugaagc aggccggcga cguggaggag aacccuggcc cuaugccuca cagcagccug 300 cacccuagca ucccuugccc uagaggccac ggcgcccaga aggccgcccu ggugcugcug 360 agcgccugcc uggugacccu guggggccug ggcgagccuc cugagcacac ccugagauac 420 cuaguuuugc accuggcuuc ucugcagcug ggccuacugc ucaacggcgu gugcagccug 480 gccgaggagc ugagacacau ccacagcaga uacagaggca gcuacuggag aaccgugaga 540 gcaugcuuag gcugcccucu gagaagaggc gcucugcucc ucuuguccau cuacuucuac 600 uacucgcuac cuaacgccgu gggcccuccu uucaccugga ugcuggcccu cuugggauua 660 agccaggccc ugaacaucuu gcugggacug aagggccugg ccccugccga gaucagcgcc 720 gugugcgaga agggcaacuu caacauggcc cacggacucg cuuggagcua cuacaucggc 780 uaccugagac ugauccugcc ugagcugcag gccagaauca gaaccuacaa ccagcacuac 840 aacaaccugc ugcggggagc agugagccag agacuguaua uucugcuccc ucuggacugc 900 ggcgugccug acaaccugag cauggccgac ccuaacauca gauuccugga caagcugccu 960 cagcagaccg gcgaccacgc cggcaucaag gacagagugu acagcaacag cauuuacgag 1020 cugcuggaga acggccagag agccggcacc ugcgugcugg aguacgccac cccucugcag 1080 acccuguucg ccaugagcca guacucccag gcaggauuca gcagagagga cagacuggag 1140 caggccaagc uguucugccg uacucuugag gacauccuug cagacgcccc ugagagccag 1200 aacaacugcc gguugauugc cuaccaggaa ccggcagacg acagcucauu cuccuugucu 1260 caggaggucc uuagacaccu gcggcaggag gagaaggagg aggugaccgu gggcagccug 1320 aagacauccg ccgugccuag cacgucuacc augucccagg agccggaacu gcuaaucagc 1380 ggcauggaga agccucugcc ucucaggacc gacuucagc 1419 < 210 > 225 < 211 > 1140 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic : Hu STING ( R 284 K ) var ; no epitope tag < 400 > 225 augccccaua gcagccugca ccccagcauc cccugcccca gaggccacgg cgcccagaag 60 gccgcccugg uccugcugag cgcaugccug gucacccugu ggggccuggg cgagcccccc 120 gagcacaccc ugagauaccu ggugcugcac cucgccagcc ugcagcuggg ccugcugcug 180 aacggcgugu gcagccuggc cgaggagcug agacacaucc acagcagaua uagaggcagc 240 uacuggagaa ccgugagagc uugccucggc ugcccccuga gaagaggcgc ccugcugcug 300 cugagcaucu acuuuuacua cagccugccc aacgcugugg gccccccuuu cacguggaug 360 cucgcccugc ugggacugag ccaggcccug aacauccugc ugggccuuaa gggccuagcc 420 cccgccgaga ucagcgccgu gugcgagaag ggcaacuuca auguggccca cggccuggcc 480 uggagcuacu acaucggcua ccugagacug auccugcccg agcugcaggc cagaaucaga 540 accuacaauc agcacuacaa caaccugcug agaggcgccg ugagccagag acuguacauc 600 cugcugcccc uggacugcgg cgugcccgac aaccucagca uggccgaccc caacaucaga 660 uuccuggaca agcugcccca gcagaccggc gaccacgccg gcaucaagga ucgcguguac 720 agcaacagca ucuacgagcu gcuggaaaac ggccagagag ccggaaccug cgugcuggag 780 uacgccacac cccugcagac ccuguucgcc augagccagu acagccaggc cggcuucagc 840 agagaggaca agcuggagca ggccaagcug uucugcagaa cccuggagga uauccucgcc 900 gacgcccccg agagccagaa caacugcagg cugaucgcgu accaggagcc cgcugacgac 960 agcagcuuua gccugagcca ggaggugcug agacaucugc gucaagagga aaaggaggag 1020 gugaccgugg gcucccugaa gaccagcgcc gugcccagca ccagcaccau gagccaggag 1080 cccgagcugc ugaucagcgg cauggagaag ccacugcccc ucagaaccga cuucagcacc 1140 < 210 > 226 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : EBV BRLF 1 peptide < 400 > 226 Ala Thr Ile Gly Thr Ala Met Tyr Lys 1 5 < 210 > 227 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : FLU peptide < 400 > 227 Ser Ile Ile Pro Ser Gly Pro Leu Lys 1 5 < 210 > 228 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : HIV NEF peptide < 400 > 228 Ala Val Asp Leu Ser His Phe Leu Lys 1 5 < 210 > 229 < 211 > 10 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : EBV peptide < 400 > 229 Ala Val Phe Asp Arg Lys Ser Asp Ala Lys 1 5 10 < 210 > 230 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : HBV core antigen peptide < 400 > 230 Tyr Val Asn Val Asn Met Gly Leu Lys 1 5 < 210 > 231 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : HC peptide < 400 > 231 Arg Val Cys Glu Lys Met Ala Leu Tyr 1 5 < 210 > 232 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic : CMV peptide < 400 > 232 Lys Leu Gly Gly Ala Leu Gln Ala Lys 1 5