SEQUENCE LISTING < 110 > GILEAD BIOLOGICS , INC . MCCAULEY , Scott Alan KOVALENKO , Maria < 120 > ANTIBODIES TO MATRIX METALLOPROTEINASE 9 < 130 > 246102008040 < 140 > PCT / US 2011 / 049448 < 141 > 2011 - 08 - 26 < 150 > US 61 / 377 , 886 < 151 > 2010 - 08 - 27 < 160 > 29 < 170 > FastSEQ for Windows Version 4 . 0 < 210 > 1 < 211 > 470 < 212 > PRT < 213 > Mus musculus < 220 > < 221 > CHAIN < 222 > ( 1 ) . ..( 470 ) < 223 > AB 0041 heavy chain < 220 > < 221 > PEPTIDE < 222 > ( 1 ) . ..( 19 ) < 223 > signal peptide < 220 > < 221 > misc _ feature < 222 > ( 135 ) . ..( 470 ) < 223 > IgG 2 b constant region < 400 > 1 Met Ala Val Leu Val Leu Phe Leu Cys Leu Val Ala Phe Pro Ser Cys 1 5 10 15 Val Leu Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Ala 20 25 30 Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu 35 40 45 Leu Ser Tyr Gly Val His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu 50 55 60 Glu Trp Leu Gly Val Ile Trp Thr Gly Gly Thr Thr Asn Tyr Asn Ser 65 70 75 80 Ala Leu Met Ser Arg Leu Ser Ile Ser Lys Asp Asp Ser Lys Ser Gln 85 90 95 Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr 100 105 110 Tyr Cys Ala Arg Tyr Tyr Tyr Gly Met Asp Tyr Trp Gly Gln Gly Thr 115 120 125 Ser Val Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro 130 135 140 Leu Ala Pro Gly Cys Gly Asp Thr Thr Gly Ser Ser Val Thr Leu Gly 145 150 155 160 Cys Leu Val Lys Gly Tyr Phe Pro Glu Ser Val Thr Val Thr Trp Asn 165 170 175 Ser Gly Ser Leu Ser Ser Ser Val His Thr Phe Pro Ala Leu Leu Gln 180 185 190 Ser Gly Leu Tyr Thr Met Ser Ser Ser Val Thr Val Pro Ser Ser Thr 195 200 205 Trp Pro Ser Gln Thr Val Thr Cys Ser Val Ala His Pro Ala Ser Ser 210 215 220 Thr Thr Val Asp Lys Lys Leu Glu Pro Ser Gly Pro Ile Ser Thr Ile 225 230 235 240 Asn Pro Cys Pro Pro Cys Lys Glu Cys His Lys Cys Pro Ala Pro Asn 245 250 255 Leu Glu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Asn Ile Lys Asp 260 265 270 Val Leu Met Ile Ser Leu Thr Pro Lys Val Thr Cys Val Val Val Asp 275 280 285 Val Ser Glu Asp Asp Pro Asp Val Arg Ile Ser Trp Phe Val Asn Asn 290 295 300 Val Glu Val His Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn 305 310 315 320 Ser Thr Ile Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp 325 330 335 Met Ser Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro 340 345 350 Ser Pro Ile Glu Arg Thr Ile Ser Lys Ile Lys Gly Leu Val Arg Ala 355 360 365 Pro Gln Val Tyr Ile Leu Pro Pro Pro Ala Glu Gln Leu Ser Arg Lys 370 375 380 Asp Val Ser Leu Thr Cys Leu Val Val Gly Phe Asn Pro Gly Asp Ile 385 390 395 400 Ser Val Glu Trp Thr Ser Asn Gly His Thr Glu Glu Asn Tyr Lys Asp 405 410 415 Thr Ala Pro Val Leu Asp Ser Asp Gly Ser Tyr Phe Ile Tyr Ser Lys 420 425 430 Leu Asp Ile Lys Thr Ser Lys Trp Glu Lys Thr Asp Ser Phe Ser Cys 435 440 445 Asn Val Arg His Glu Gly Leu Lys Asn Tyr Tyr Leu Lys Lys Thr Ile 450 455 460 Ser Arg Ser Pro Gly Lys 465 470 < 210 > 2 < 211 > 234 < 212 > PRT < 213 > Mus musculus < 220 > < 221 > CHAIN < 222 > ( 1 ) . ..( 234 ) < 223 > AB 0041 light chain < 220 > < 221 > PEPTIDE < 222 > ( 1 ) . ..( 20 ) < 223 > signal peptide < 220 > < 221 > misc _ feature < 222 > ( 128 ) . ..( 234 ) < 223 > kappa constant region < 400 > 2 Met Glu Ser Gln Ile Gln Val Phe Val Phe Val Phe Leu Trp Leu Ser 1 5 10 15 Gly Val Asp Gly Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser 20 25 30 Thr Ser Val Gly Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp 35 40 45 Val Arg Asn Thr Val Ala Trp Tyr Gln Gln Lys Thr Gly Gln Ser Pro 50 55 60 Lys Leu Leu Ile Tyr Ser Ser Ser Tyr Arg Asn Thr Gly Val Pro Asp 65 70 75 80 Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser 85 90 95 Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln His Tyr 100 105 110 Ile Thr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 115 120 125 Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln 130 135 140 Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr 145 150 155 160 Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln 165 170 175 Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr 180 185 190 Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg 195 200 205 His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro 210 215 220 Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 225 230 < 210 > 3 < 211 > 115 < 212 > PRT < 213 > Mus musculus < 220 > < 221 > CHAIN < 222 > ( 1 ) . ..( 115 ) < 223 > variable region of the IgG 2 b heavy chain of AB 0041 < 220 > < 221 > misc _ feature < 222 > ( 26 ) . ..( 35 ) < 223 > complementarity - determining region ( CDR ) < 220 > < 221 > misc _ feature < 222 > ( 50 ) . ..( 65 ) < 223 > complementarity - determining region ( CDR ) < 220 > < 221 > misc _ feature < 222 > ( 98 ) . ..( 104 ) < 223 > complementarity - determining region ( CDR ) < 400 > 3 Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 1 5 10 15 Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Leu Ser Tyr 20 25 30 Gly Val His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 35 40 45 Gly Val Ile Trp Thr Gly Gly Thr Thr Asn Tyr Asn Ser Ala Leu Met 50 55 60 Ser Arg Leu Ser Ile Ser Lys Asp Asp Ser Lys Ser Gln Val Phe Leu 65 70 75 80 Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 85 90 95 Arg Tyr Tyr Tyr Gly Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr 100 105 110 Val Ser Ser 115 < 210 > 4 < 211 > 107 < 212 > PRT < 213 > Mus musculus < 220 > < 221 > CHAIN < 222 > ( 1 ) . ..( 107 ) < 223 > variable region of the kappa light chain of AB 0041 < 220 > < 221 > misc _ feature < 222 > ( 24 ) . ..( 34 ) < 223 > complementarity - determining region ( CDR ) < 220 > < 221 > misc _ feature < 222 > ( 50 ) . ..( 56 ) < 223 > complementarity - determining region ( CDR ) < 220 > < 221 > misc _ feature < 222 > ( 89 ) . ..( 97 ) < 223 > complementarity - determining region ( CDR ) < 400 > 4 Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly 1 5 10 15 Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Arg Asn Thr 20 25 30 Val Ala Trp Tyr Gln Gln Lys Thr Gly Gln Ser Pro Lys Leu Leu Ile 35 40 45 Tyr Ser Ser Ser Tyr Arg Asn Thr Gly Val Pro Asp Arg Phe Thr Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Val Gln Ala 65 70 75 80 Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln His Tyr Ile Thr Pro Tyr 85 90 95 Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 100 105 < 210 > 5 < 211 > 115 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > VARIANT < 222 > ( 1 ) . ..( 115 ) < 223 > VH 1 heavy chain variant < 400 > 5 Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 1 5 10 15 Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Leu Ser Tyr 20 25 30 Gly Val His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 35 40 45 Gly Val Ile Trp Thr Gly Gly Thr Thr Asn Tyr Asn Ser Ala Leu Met 50 55 60 Ser Arg Leu Thr Ile Ser Lys Asp Asp Ser Lys Ser Thr Val Tyr Leu 65 70 75 80 Lys Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Ile Tyr Tyr Cys Ala 85 90 95 Arg Tyr Tyr Tyr Gly Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr 100 105 110 Val Ser Ser 115 < 210 > 6 < 211 > 115 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > VARIANT < 222 > ( 1 ) . ..( 115 ) < 223 > VH 2 heavy chain variant < 400 > 6 Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 1 5 10 15 Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Leu Ser Tyr 20 25 30 Gly Val His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 35 40 45 Gly Val Ile Trp Thr Gly Gly Thr Thr Asn Tyr Asn Ser Ala Leu Met 50 55 60 Ser Arg Leu Thr Ile Ser Lys Asp Asp Ser Lys Asn Thr Val Tyr Leu 65 70 75 80 Lys Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Ile Tyr Tyr Cys Ala 85 90 95 Arg Tyr Tyr Tyr Gly Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 100 105 110 Val Ser Ser 115 < 210 > 7 < 211 > 115 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > VARIANT < 222 > ( 1 ) . ..( 115 ) < 223 > VH 3 heavy chain variant < 400 > 7 Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 1 5 10 15 Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Leu Ser Tyr 20 25 30 Gly Val His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 35 40 45 Gly Val Ile Trp Thr Gly Gly Thr Thr Asn Tyr Asn Ser Ala Leu Met 50 55 60 Ser Arg Phe Thr Ile Ser Lys Asp Asp Ser Lys Asn Thr Val Tyr Leu 65 70 75 80 Lys Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Ile Tyr Tyr Cys Ala 85 90 95 Arg Tyr Tyr Tyr Gly Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 100 105 110 Val Ser Ser 115 < 210 > 8 < 211 > 115 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > VARIANT < 222 > ( 1 ) . ..( 115 ) < 223 > VH 4 heavy chain variant < 400 > 8 Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 1 5 10 15 Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Leu Ser Tyr 20 25 30 Gly Val His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 35 40 45 Gly Val Ile Trp Thr Gly Gly Thr Thr Asn Tyr Asn Ser Ala Leu Met 50 55 60 Ser Arg Phe Thr Ile Ser Lys Asp Asp Ser Lys Asn Thr Leu Tyr Leu 65 70 75 80 Lys Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Ile Tyr Tyr Cys Ala 85 90 95 Arg Tyr Tyr Tyr Gly Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 100 105 110 Val Ser Ser 115 < 210 > 9 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > VARIANT < 222 > ( 1 ) . ..( 107 ) < 223 > Vk 1 light chain variant < 400 > 9 Asp Ile Val Met Thr Gln Ser Pro Ser Phe Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Arg Asn Thr 20 25 30 Val Ala Trp Tyr Gln Gln Lys Thr Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Ser Ser Ser Tyr Arg Asn Thr Gly Val Pro Asp Arg Phe Thr Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala 65 70 75 80 Glu Asp Val Ala Val Tyr Phe Cys Gln Gln His Tyr Ile Thr Pro Tyr 85 90 95 Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 10 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > VARIANT < 222 > ( 1 ) . ..( 107 ) < 223 > Vk 2 light chain variant < 400 > 10 Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Arg Asn Thr 20 25 30 Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Ser Ser Ser Tyr Arg Asn Thr Gly Val Pro Asp Arg Phe Thr Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala 65 70 75 80 Glu Asp Val Ala Val Tyr Phe Cys Gln Gln His Tyr Ile Thr Pro Tyr 85 90 95 Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 11 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > VARIANT < 222 > ( 1 ) . ..( 107 ) < 223 > Vk 3 light chain variant < 400 > 11 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Arg Asn Thr 20 25 30 Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Ser Ser Ser Tyr Arg Asn Thr Gly Val Pro Asp Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala 65 70 75 80 Glu Asp Val Ala Val Tyr Phe Cys Gln Gln His Tyr Ile Thr Pro Tyr 85 90 95 Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 12 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > VARIANT < 222 > ( 1 ) . ..( 107 ) < 223 > Vk 4 light chain variant < 400 > 12 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Arg Asn Thr 20 25 30 Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Ser Ser Ser Tyr Arg Asn Thr Gly Val Pro Asp Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala 65 70 75 80 Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln His Tyr Ile Thr Pro Tyr 85 90 95 Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 13 < 211 > 10 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 10 ) < 223 > complementarity - determining region ( CDR 1 ) of heavy chain of anti - MMP 9 antibody < 400 > 13 Gly Phe Ser Leu Leu Ser Tyr Gly Val His 1 5 10 < 210 > 14 < 211 > 16 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 16 ) < 223 > complementarity - determining region ( CDR 2 ) of heavy chain of anti - MMP 9 antibody < 400 > 14 Val Ile Trp Thr Gly Gly Thr Thr Asn Tyr Asn Ser Ala Leu Met Ser 1 5 10 15 < 210 > 15 < 211 > 7 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 7 ) < 223 > complementarity - determining region ( CDR 3 ) of heavy chain of anti - MMP 9 antibody < 400 > 15 Tyr Tyr Tyr Gly Met Asp Tyr 1 5 < 210 > 16 < 211 > 11 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 11 ) < 223 > complementarity - determining region ( CDR 1 ) of light chain of anti - MMP 9 antibody < 400 > 16 Lys Ala Ser Gln Asp Val Arg Asn Thr Val Ala 1 5 10 < 210 > 17 < 211 > 7 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 7 ) < 223 > complementarity - determining region ( CDR 2 ) of light chain of anti - MMP 9 antibody < 400 > 17 Ser Ser Ser Tyr Arg Asn Thr 1 5 < 210 > 18 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 9 ) < 223 > complementarity - determining region ( CDR 3 ) of light chain of anti - MMP 9 antibody < 400 > 18 Gln Gln His Tyr Ile Thr Pro Tyr Thr 1 5 < 210 > 19 < 211 > 345 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 345 ) < 223 > nucleotide sequence encoding VH 1 heavy chain amino acid sequence < 400 > 19 caggtgcagc tgcaggaatc cggccctggc ctggtcaagc cctccgagac actgtccctg 60 acctgcaccg tgtccggctt ctccctgctg tcctacggcg tgcactgggt ccgacagcct 120 ccagggaagg gcctggaatg gctgggcgtg atctggaccg gcggcaccac caactacaac 180 tccgccctga tgtcccggct gaccatctcc aaggacgact ccaagtccac cgtgtacctg 240 aagatgaact ccctgaaaac cgaggacacc gccatctact actgcgcccg gtactactac 300 ggcatggact actggggcca gggcacctcc gtgaccgtgt cctca 345 < 210 > 20 < 211 > 345 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 345 ) < 223 > nucleotide sequence encoding VH 2 heavy chain amino acid sequence < 400 > 20 caggtgcagc tgcaggaatc cggccctggc ctggtcaagc cctccgagac actgtccctg 60 acctgcaccg tgtccggctt ctccctgctg tcctacggcg tgcactgggt ccgacagcct 120 ccaggcaaag gcctggaatg gctgggcgtg atctggaccg gcggcaccac caactacaac 180 tccgccctga tgtcccggct gaccatctcc aaggacgact ccaagaacac cgtgtacctg 240 aagatgaact ccctgaaaac cgaggacacc gccatctact actgcgcccg gtactactac 300 ggcatggact actggggcca gggcaccctg gtcaccgtgt cctca 345 < 210 > 21 < 211 > 345 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 345 ) < 223 > nucleotide sequence encoding VH 3 heavy chain amino acid sequence < 400 > 21 caggtgcagc tgcaggaatc cggccctggc ctggtcaagc cctccgagac actgtccctg 60 acctgcaccg tgtccggctt ctccctgctg tcctacggcg tgcactgggt ccgacagcct 120 ccaggcaaag gcctggaatg gctgggcgtg atctggaccg gcggcaccac caactacaac 180 tccgccctga tgtcccggtt caccatctcc aaggacgact ccaagaacac cgtgtacctg 240 aagatgaact ccctgaaaac cgaggacacc gccatctact actgcgcccg gtactactac 300 ggcatggact actggggcca gggcaccctg gtcaccgtgt cctca 345 < 210 > 22 < 211 > 345 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 345 ) < 223 > nucleotide sequence encoding VH 4 heavy chain amino acid sequence < 400 > 22 caggtgcagc tgcaggaatc cggccctggc ctggtcaagc cctccgagac actgtccctg 60 acctgcaccg tgtccggctt ctccctgctg tcctacggcg tgcactgggt ccgacagcct 120 ccaggcaaag gcctggaatg gctgggcgtg atctggaccg gcggcaccac caactacaac 180 tccgccctga tgtcccggtt caccatctcc aaggacgact ccaagaacac cctgtacctg 240 aagatgaact ccctgaaaac cgaggacacc gccatctact actgcgcccg gtactactac 300 ggcatggact actggggcca gggcaccctg gtcaccgtgt cctca 345 < 210 > 23 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 321 ) < 223 > nucleotide sequence encoding Vk 1 light chain amino acid sequence < 400 > 23 gacatcgtga tgacccagtc ccccagcttc ctgtccgcct ccgtgggcga cagagtgacc 60 atcacatgca aggcctctca ggacgtgcgg aacaccgtgg cctggtatca gcagaaaacc 120 ggcaaggccc ccaagctgct gatctactcc tcctcctacc ggaacaccgg cgtgcccgac 180 cggtttaccg gctctggctc cggcaccgac tttaccctga ccatcagctc cctgcaggcc 240 gaggacgtgg ccgtgtactt ctgccagcag cactacatca ccccctacac cttcggcgga 300 ggcaccaagg tggaaataaa a 321 < 210 > 24 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 321 ) < 223 > nucleotide sequence encoding Vk 2 light chain amino acid sequence < 400 > 24 gacatcgtga tgacccagtc cccctccagc ctgtccgcct ctgtgggcga cagagtgacc 60 atcacatgca aggcctctca ggacgtgcgg aacaccgtgg cctggtatca gcagaagccc 120 ggcaaggccc ccaagctgct gatctactcc tcctcctacc ggaacaccgg cgtgcccgac 180 cggtttaccg gctctggctc cggcaccgac tttaccctga ccatcagctc cctgcaggcc 240 gaggacgtgg ccgtgtactt ctgccagcag cactacatca ccccctacac cttcggcgga 300 ggcaccaagg tggaaataaa a 321 < 210 > 25 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 321 ) < 223 > nucleotide sequence encoding Vk 3 light chain amino acid sequence < 400 > 25 gacatccaga tgacccagtc cccctccagc ctgtccgcct ctgtgggcga cagagtgacc 60 atcacatgca aggcctccca ggacgtgcgg aacaccgtgg cctggtatca gcagaagccc 120 ggcaaggccc ccaagctgct gatctactcc tcctcctacc ggaacaccgg cgtgcccgac 180 cggttctctg gctctggaag cggcaccgac tttaccctga ccatcagctc cctgcaggcc 240 gaggacgtgg ccgtgtactt ctgccagcag cactacatca ccccctacac cttcggcgga 300 ggcaccaagg tggaaataaa a 321 < 210 > 26 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > synthetic construct < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 321 ) < 223 > nucleotide sequence encoding Vk 4 light chain amino acid sequence < 400 > 26 gacatccaga tgacccagtc cccctccagc ctgtccgcct ctgtgggcga cagagtgacc 60 atcacatgca aggcctctca ggacgtgcgg aacaccgtgg cctggtatca gcagaagccc 120 ggcaaggccc ccaagctgct gatctactcc tcctcctacc ggaacaccgg cgtgcccgac 180 cggttctctg gctctggaag cggcaccgac tttaccctga ccatcagctc cctgcaggcc 240 gaggacgtgg ccgtgtacta ctgccagcag cactacatca ccccctacac cttcggcgga 300 ggcaccaagg tggaaataaa a 321 < 210 > 27 < 211 > 707 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 707 ) < 223 > matrix metalloproteinase 9 ( MMP 9 ) < 220 > < 221 > PEPTIDE < 222 > ( 1 ) . ..( 19 ) < 223 > signal peptide < 220 > < 221 > DOMAIN < 222 > ( 38 ) . ..( 98 ) < 223 > peptidoglycan binding domain < 220 > < 221 > SITE < 222 > ( 98 ) . ..( 99 ) < 223 > propeptide cleavage site < 220 > < 221 > DOMAIN < 222 > ( 112 ) . ..( 445 ) < 223 > Zn dependent metalloproteinase domain < 220 > < 221 > DOMAIN < 222 > ( 223 ) . ..( 271 ) < 223 > fibronectin type II domain ( gelatin binding domain ) < 220 > < 221 > DOMAIN < 222 > ( 281 ) . ..( 329 ) < 223 > fibronectin type II domain ( gelatin binding domain ) < 220 > < 221 > DOMAIN < 222 > ( 340 ) . ..( 388 ) < 223 > fibronectin type II domain ( gelatin binding domain ) < 220 > < 221 > misc _ feature < 222 > ( 400 ) . ..( 411 ) < 223 > Zn binding region < 220 > < 221 > DOMAIN < 222 > ( 521 ) . ..( 565 ) < 223 > hemopexin - like domain < 220 > < 221 > DOMAIN < 222 > ( 567 ) . ..( 608 ) < 223 > hemopexin - like domain < 220 > < 221 > DOMAIN < 222 > ( 613 ) . ..( 659 ) < 223 > hemopexin - like domain < 220 > < 221 > DOMAIN < 222 > ( 661 ) . ..( 704 ) < 223 > hemopexin - like domain < 400 > 27 Met Ser Leu Trp Gln Pro Leu Val Leu Val Leu Leu Val Leu Gly Cys 1 5 10 15 Cys Phe Ala Ala Pro Arg Gln Arg Gln Ser Thr Leu Val Leu Phe Pro 20 25 30 Gly Asp Leu Arg Thr Asn Leu Thr Asp Arg Gln Leu Ala Glu Glu Tyr 35 40 45 Leu Tyr Arg Tyr Gly Tyr Thr Arg Val Ala Glu Met Arg Gly Glu Ser 50 55 60 Lys Ser Leu Gly Pro Ala Leu Leu Leu Leu Gln Lys Gln Leu Ser Leu 65 70 75 80 Pro Glu Thr Gly Glu Leu Asp Ser Ala Thr Leu Lys Ala Met Arg Thr 85 90 95 Pro Arg Cys Gly Val Pro Asp Leu Gly Arg Phe Gln Thr Phe Glu Gly 100 105 110 Asp Leu Lys Trp His His His Asn Ile Thr Tyr Trp Ile Gln Asn Tyr 115 120 125 Ser Glu Asp Leu Pro Arg Ala Val Ile Asp Asp Ala Phe Ala Arg Ala 130 135 140 Phe Ala Leu Trp Ser Ala Val Thr Pro Leu Thr Phe Thr Arg Val Tyr 145 150 155 160 Ser Arg Asp Ala Asp Ile Val Ile Gln Phe Gly Val Ala Glu His Gly 165 170 175 Asp Gly Tyr Pro Phe Asp Gly Lys Asp Gly Leu Leu Ala His Ala Phe 180 185 190 Pro Pro Gly Pro Gly Ile Gln Gly Asp Ala His Phe Asp Asp Asp Glu 195 200 205 Leu Trp Ser Leu Gly Lys Gly Val Val Val Pro Thr Arg Phe Gly Asn 210 215 220 Ala Asp Gly Ala Ala Cys His Phe Pro Phe Ile Phe Glu Gly Arg Ser 225 230 235 240 Tyr Ser Ala Cys Thr Thr Asp Gly Arg Ser Asp Gly Leu Pro Trp Cys 245 250 255 Ser Thr Thr Ala Asn Tyr Asp Thr Asp Asp Arg Phe Gly Phe Cys Pro 260 265 270 Ser Glu Arg Leu Tyr Thr Arg Asp Gly Asn Ala Asp Gly Lys Pro Cys 275 280 285 Gln Phe Pro Phe Ile Phe Gln Gly Gln Ser Tyr Ser Ala Cys Thr Thr 290 295 300 Asp Gly Arg Ser Asp Gly Tyr Arg Trp Cys Ala Thr Thr Ala Asn Tyr 305 310 315 320 Asp Arg Asp Lys Leu Phe Gly Phe Cys Pro Thr Arg Ala Asp Ser Thr 325 330 335 Val Met Gly Gly Asn Ser Ala Gly Glu Leu Cys Val Phe Pro Phe Thr 340 345 350 Phe Leu Gly Lys Glu Tyr Ser Thr Cys Thr Ser Glu Gly Arg Gly Asp 355 360 365 Gly Arg Leu Trp Cys Ala Thr Thr Ser Asn Phe Asp Ser Asp Lys Lys 370 375 380 Trp Gly Phe Cys Pro Asp Gln Gly Tyr Ser Leu Phe Leu Val Ala Ala 385 390 395 400 His Glu Phe Gly His Ala Leu Gly Leu Asp His Ser Ser Val Pro Glu 405 410 415 Ala Leu Met Tyr Pro Met Tyr Arg Phe Thr Glu Gly Pro Pro Leu His 420 425 430 Lys Asp Asp Val Asn Gly Ile Arg His Leu Tyr Gly Pro Arg Pro Glu 435 440 445 Pro Glu Pro Arg Pro Pro Thr Thr Thr Thr Pro Gln Pro Thr Ala Pro 450 455 460 Pro Thr Val Cys Pro Thr Gly Pro Pro Thr Val His Pro Ser Glu Arg 465 470 475 480 Pro Thr Ala Gly Pro Thr Gly Pro Pro Ser Ala Gly Pro Thr Gly Pro 485 490 495 Pro Thr Ala Gly Pro Ser Thr Ala Thr Thr Val Pro Leu Ser Pro Val 500 505 510 Asp Asp Ala Cys Asn Val Asn Ile Phe Asp Ala Ile Ala Glu Ile Gly 515 520 525 Asn Gln Leu Tyr Leu Phe Lys Asp Gly Lys Tyr Trp Arg Phe Ser Glu 530 535 540 Gly Arg Gly Ser Arg Pro Gln Gly Pro Phe Leu Ile Ala Asp Lys Trp 545 550 555 560 Pro Ala Leu Pro Arg Lys Leu Asp Ser Val Phe Glu Glu Pro Leu Ser 565 570 575 Lys Lys Leu Phe Phe Phe Ser Gly Arg Gln Val Trp Val Tyr Thr Gly 580 585 590 Ala Ser Val Leu Gly Pro Arg Arg Leu Asp Lys Leu Gly Leu Gly Ala 595 600 605 Asp Val Ala Gln Val Thr Gly Ala Leu Arg Ser Gly Arg Gly Lys Met 610 615 620 Leu Leu Phe Ser Gly Arg Arg Leu Trp Arg Phe Asp Val Lys Ala Gln 625 630 635 640 Met Val Asp Pro Arg Ser Ala Ser Glu Val Asp Arg Met Phe Pro Gly 645 650 655 Val Pro Leu Asp Thr His Asp Val Phe Gln Tyr Arg Glu Lys Ala Tyr 660 665 670 Phe Cys Gln Asp Arg Phe Tyr Trp Arg Val Ser Ser Arg Ser Glu Leu 675 680 685 Asn Gln Val Asp Gln Val Gly Tyr Val Thr Tyr Asp Ile Leu Gln Cys 690 695 700 Pro Glu Asp 705 < 210 > 28 < 211 > 687 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 222 > ( 1 ) . ..( 687 ) < 223 > mature full - length matrix metalloproteinase 9 ( MMP 9 ) < 400 > 28 Pro Arg Gln Arg Gln Ser Thr Leu Val Leu Phe Pro Gly Asp Leu Arg 1 5 10 15 Thr Asn Leu Thr Asp Arg Gln Leu Ala Glu Glu Tyr Leu Tyr Arg Tyr 20 25 30 Gly Tyr Thr Arg Val Ala Glu Met Arg Gly Glu Ser Lys Ser Leu Gly 35 40 45 Pro Ala Leu Leu Leu Leu Gln Lys Gln Leu Ser Leu Pro Glu Thr Gly 50 55 60 Glu Leu Asp Ser Ala Thr Leu Lys Ala Met Arg Thr Pro Arg Cys Gly 65 70 75 80 Val Pro Asp Leu Gly Arg Phe Gln Thr Phe Glu Gly Asp Leu Lys Trp 85 90 95 His His His Asn Ile Thr Tyr Trp Ile Gln Asn Tyr Ser Glu Asp Leu 100 105 110 Pro Arg Ala Val Ile Asp Asp Ala Phe Ala Arg Ala Phe Ala Leu Trp 115 120 125 Ser Ala Val Thr Pro Leu Thr Phe Thr Arg Val Tyr Ser Arg Asp Ala 130 135 140 Asp Ile Val Ile Gln Phe Gly Val Ala Glu His Gly Asp Gly Tyr Pro 145 150 155 160 Phe Asp Gly Lys Asp Gly Leu Leu Ala His Ala Phe Pro Pro Gly Pro 165 170 175 Gly Ile Gln Gly Asp Ala His Phe Asp Asp Asp Glu Leu Trp Ser Leu 180 185 190 Gly Lys Gly Val Val Val Pro Thr Arg Phe Gly Asn Ala Asp Gly Ala 195 200 205 Ala Cys His Phe Pro Phe Ile Phe Glu Gly Arg Ser Tyr Ser Ala Cys 210 215 220 Thr Thr Asp Gly Arg Ser Asp Gly Leu Pro Trp Cys Ser Thr Thr Ala 225 230 235 240 Asn Tyr Asp Thr Asp Asp Arg Phe Gly Phe Cys Pro Ser Glu Arg Leu 245 250 255 Tyr Thr Arg Asp Gly Asn Ala Asp Gly Lys Pro Cys Gln Phe Pro Phe 260 265 270 Ile Phe Gln Gly Gln Ser Tyr Ser Ala Cys Thr Thr Asp Gly Arg Ser 275 280 285 Asp Gly Tyr Arg Trp Cys Ala Thr Thr Ala Asn Tyr Asp Arg Asp Lys 290 295 300 Leu Phe Gly Phe Cys Pro Thr Arg Ala Asp Ser Thr Val Met Gly Gly 305 310 315 320 Asn Ser Ala Gly Glu Leu Cys Val Phe Pro Phe Thr Phe Leu Gly Lys 325 330 335 Glu Tyr Ser Thr Cys Thr Ser Glu Gly Arg Gly Asp Gly Arg Leu Trp 340 345 350 Cys Ala Thr Thr Ser Asn Phe Asp Ser Asp Lys Lys Trp Gly Phe Cys 355 360 365 Pro Asp Gln Gly Tyr Ser Leu Phe Leu Val Ala Ala His Glu Phe Gly 370 375 380 His Ala Leu Gly Leu Asp His Ser Ser Val Pro Glu Ala Leu Met Tyr 385 390 395 400 Pro Met Tyr Arg Phe Thr Glu Gly Pro Pro Leu His Lys Asp Asp Val 405 410 415 Asn Gly Ile Arg His Leu Tyr Gly Pro Arg Pro Glu Pro Glu Pro Arg 420 425 430 Pro Pro Thr Thr Thr Thr Pro Gln Pro Thr Ala Pro Pro Thr Val Cys 435 440 445 Pro Thr Gly Pro Pro Thr Val His Pro Ser Glu Arg Pro Thr Ala Gly 450 455 460 Pro Thr Gly Pro Pro Ser Ala Gly Pro Thr Gly Pro Pro Thr Ala Gly 465 470 475 480 Pro Ser Thr Ala Thr Thr Val Pro Leu Ser Pro Val Asp Asp Ala Cys 485 490 495 Asn Val Asn Ile Phe Asp Ala Ile Ala Glu Ile Gly Asn Gln Leu Tyr 500 505 510 Leu Phe Lys Asp Gly Lys Tyr Trp Arg Phe Ser Glu Gly Arg Gly Ser 515 520 525 Arg Pro Gln Gly Pro Phe Leu Ile Ala Asp Lys Trp Pro Ala Leu Pro 530 535 540 Arg Lys Leu Asp Ser Val Phe Glu Glu Pro Leu Ser Lys Lys Leu Phe 545 550 555 560 Phe Phe Ser Gly Arg Gln Val Trp Val Tyr Thr Gly Ala Ser Val Leu 565 570 575 Gly Pro Arg Arg Leu Asp Lys Leu Gly Leu Gly Ala Asp Val Ala Gln 580 585 590 Val Thr Gly Ala Leu Arg Ser Gly Arg Gly Lys Met Leu Leu Phe Ser 595 600 605 Gly Arg Arg Leu Trp Arg Phe Asp Val Lys Ala Gln Met Val Asp Pro 610 615 620 Arg Ser Ala Ser Glu Val Asp Arg Met Phe Pro Gly Val Pro Leu Asp 625 630 635 640 Thr His Asp Val Phe Gln Tyr Arg Glu Lys Ala Tyr Phe Cys Gln Asp 645 650 655 Arg Phe Tyr Trp Arg Val Ser Ser Arg Ser Glu Leu Asn Gln Val Asp 660 665 670 Gln Val Gly Tyr Val Thr Tyr Asp Ile Leu Gln Cys Pro Glu Asp 675 680 685 < 210 > 29 < 211 > 20 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > PEPTIDE < 222 > ( 1 ) . ..( 20 ) < 223 > matrix metalloproteinase 9 ( MMP 9 ) signal peptide < 400 > 29 Met Ser Leu Trp Gln Pro Leu Val Leu Val Leu Leu Val Leu Gly Cys 1 5 10 15 Cys Phe Ala Ala 20