신생아 중 환자실 에서 의 아데노바이러스 8 형 에 의한 유행 성 각 결막염 의 발생 
 1 Department of Pediatrics , Seoul National University , College of Medicine , Seoul , Korea . kimhans @ snu . ac . kr 
 2 Department of Ophthalmology , Seoul National University , College of Medicine , Seoul , Korea . 
 3 Infection Control Service , Seoul National University Hospital , Seoul , Korea . 
 Epidemic keratoconjunctivitis ( EKC ) caused by adenovirus is a highly contagious disease , which has been reported as outbreaks involving adults in the community . However , there has been no report on EKC outbreak by adenovirus in a neonatal intensive care unit ( NICU ) in Korea . Aims of this study were to investigate the EKC outbreak by adenovirus type 8 in NICU and to confirm an effectiveness of polymerase chain reaction ( PCR ) for diagnosis . 
 Conjunctival swab or nasopharyngeal aspirate specimens were taken from all patients and tested by viral culture and PCR . Adenovirus serotype was determined by sequencing of PCR product of selected region of hexon gene using the virus isolates or specimens . 
 An outbreak of EKC occurred which was involving 12 preterm infants in the NICU of the Seoul National University Children ' s Hospital between July 12 th and August 1 st , 2005 . Three hospital staffs and one family member of the neonate were also affected . Adenovirus was detected in 12 / 12 ( 100 %), 6 / 11 ( 54 . 5 %) by PCR and virus culture , respectively . Eleven PCR - positive neonates were identified as serotype 8 by sequencing . The first affected 4 babies have had routine ROP ( retinopathy of prematurity ) examinations one week ago . While previous outbreaks were sustained for a few months , the event in our unit was controlled without complications in 3 weeks . 
 We analyzed the EKC outbreak by adenovirus type 8 in NICU . Adenovirus serotype was identified by PCR and sequencing with high sensitivity for the first time in Korea , so we suggest this method can be very useful for rapid diagnosis and infection control . 
 Key Words : Epidemic keratoconjunctivitis , Adenovirus , Neonatal intensive care units , Premature infant