SEQUENCE LISTING < 110 > Matthiessen , et al . < 120 > Recombinant VWF Formulations < 130 > 31315 / 43550 < 150 > 61 / 017 , 881 < 151 > 2007 - 12 - 31 < 160 > 3 < 170 > PatentIn version 3 . 5 < 210 > 1 < 211 > 8833 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Synthetic Polynucleotide < 220 > < 221 > misc _ feature < 223 > prepro - vWF < 400 > 1 agctcacagc tattgtggtg ggaaagggag ggtggttggt ggatgtcaca gcttgggctt 60 tatctccccc agcagtgggg actccacagc ccctgggcta cataacagca agacagtccg 120 gagctgtagc agacctgatt gagcctttgc agcagctgag agcatggcct agggtgggcg 180 gcaccattgt ccagcagctg agtttcccag ggaccttgga gatagccgca gccctcattt 240 gcaggggaag atgattcctg ccagatttgc cggggtgctg cttgctctgg ccctcatttt 300 gccagggacc ctttgtgcag aaggaactcg cggcaggtca tccacggccc gatgcagcct 360 tttcggaagt gacttcgtca acacctttga tgggagcatg tacagctttg cgggatactg 420 cagttacctc ctggcagggg gctgccagaa acgctccttc tcgattattg gggacttcca 480 gaatggcaag agagtgagcc tctccgtgta tcttggggaa ttttttgaca tccatttgtt 540 tgtcaatggt accgtgacac agggggacca aagagtctcc atgccctatg cctccaaagg 600 gctgtatcta gaaactgagg ctgggtacta caagctgtcc ggtgaggcct atggctttgt 660 ggccaggatc gatggcagcg gcaactttca agtcctgctg tcagacagat acttcaacaa 720 gacctgcggg ctgtgtggca actttaacat ctttgctgaa gatgacttta tgacccaaga 780 agggaccttg acctcggacc cttatgactt tgccaactca tgggctctga gcagtggaga 840 acagtggtgt gaacgggcat ctcctcccag cagctcatgc aacatctcct ctggggaaat 900 gcagaagggc ctgtgggagc agtgccagct tctgaagagc acctcggtgt ttgcccgctg 960 ccaccctctg gtggaccccg agccttttgt ggccctgtgt gagaagactt tgtgtgagtg 1020 tgctgggggg ctggagtgcg cctgccctgc cctcctggag tacgcccgga cctgtgccca 1080 ggagggaatg gtgctgtacg gctggaccga ccacagcgcg tgcagcccag tgtgccctgc 1140 tggtatggag tataggcagt gtgtgtcccc ttgcgccagg acctgccaga gcctgcacat 1200 caatgaaatg tgtcaggagc gatgcgtgga tggctgcagc tgccctgagg gacagctcct 1260 ggatgaaggc ctctgcgtgg agagcaccga gtgtccctgc gtgcattccg gaaagcgcta 1320 ccctcccggc acctccctct ctcgagactg caacacctgc atttgccgaa acagccagtg 1380 gatctgcagc aatgaagaat gtccagggga gtgccttgtc acaggtcaat cacacttcaa 1440 gagctttgac aacagatact tcaccttcag tgggatctgc cagtacctgc tggcccggga 1500 ttgccaggac cactccttct ccattgtcat tgagactgtc cagtgtgctg atgaccgcga 1560 cgctgtgtgc acccgctccg tcaccgtccg gctgcctggc ctgcacaaca gccttgtgaa 1620 actgaagcat ggggcaggag ttgccatgga tggccaggac gtccagctcc ccctcctgaa 1680 aggtgacctc cgcatccagc atacagtgac ggcctccgtg cgcctcagct acggggagga 1740 cctgcagatg gactgggatg gccgcgggag gctgctggtg aagctgtccc ccgtctatgc 1800 cgggaagacc tgcggcctgt gtgggaatta caatggcaac cagggcgacg acttccttac 1860 cccctctggg ctggcggagc cccgggtgga ggacttcggg aacgcctgga agctgcacgg 1920 ggactgccag gacctgcaga agcagcacag cgatccctgc gccctcaacc cgcgcatgac 1980 caggttctcc gaggaggcgt gcgcggtcct gacgtccccc acattcgagg cctgccatcg 2040 tgccgtcagc ccgctgccct acctgcggaa ctgccgctac gacgtgtgct cctgctcgga 2100 cggccgcgag tgcctgtgcg gcgccctggc cagctatgcc gcggcctgcg cggggagagg 2160 cgtgcgcgtc gcgtggcgcg agccaggccg ctgtgagctg aactgcccga aaggccaggt 2220 gtacctgcag tgcgggaccc cctgcaacct gacctgccgc tctctctctt acccggatga 2280 ggaatgcaat gaggcctgcc tggagggctg cttctgcccc ccagggctct acatggatga 2340 gaggggggac tgcgtgccca aggcccagtg cccctgttac tatgacggtg agatcttcca 2400 gccagaagac atcttctcag accatcacac catgtgctac tgtgaggatg gcttcatgca 2460 ctgtaccatg agtggagtcc ccggaagctt gctgcctgac gctgtcctca gcagtcccct 2520 gtctcatcgc agcaaaagga gcctatcctg tcggcccccc atggtcaagc tggtgtgtcc 2580 cgctgacaac ctgcgggctg aagggctcga gtgtaccaaa acgtgccaga actatgacct 2640 ggagtgcatg agcatgggct gtgtctctgg ctgcctctgc cccccgggca tggtccggca 2700 tgagaacaga tgtgtggccc tggaaaggtg tccctgcttc catcagggca aggagtatgc 2760 ccctggagaa acagtgaaga ttggctgcaa cacttgtgtc tgtcgggacc ggaagtggaa 2820 ctgcacagac catgtgtgtg atgccacgtg ctccacgatc ggcatggccc actacctcac 2880 cttcgacggg ctcaaatacc tgttccccgg ggagtgccag tacgttctgg tgcaggatta 2940 ctgcggcagt aaccctggga cctttcggat cctagtgggg aataagggat gcagccaccc 3000 ctcagtgaaa tgcaagaaac gggtcaccat cctggtggag ggaggagaga ttgagctgtt 3060 tgacggggag gtgaatgtga agaggcccat gaaggatgag actcactttg aggtggtgga 3120 gtctggccgg tacatcattc tgctgctggg caaagccctc tccgtggtct gggaccgcca 3180 cctgagcatc tccgtggtcc tgaagcagac ataccaggag aaagtgtgtg gcctgtgtgg 3240 gaattttgat ggcatccaga acaatgacct caccagcagc aacctccaag tggaggaaga 3300 ccctgtggac tttgggaact cctggaaagt gagctcgcag tgtgctgaca ccagaaaagt 3360 gcctctggac tcatcccctg ccacctgcca taacaacatc atgaagcaga cgatggtgga 3420 ttcctcctgt agaatcctta ccagtgacgt cttccaggac tgcaacaagc tggtggaccc 3480 cgagccatat ctggatgtct gcatttacga cacctgctcc tgtgagtcca ttggggactg 3540 cgcctgcttc tgcgacacca ttgctgccta tgcccacgtg tgtgcccagc atggcaaggt 3600 ggtgacctgg aggacggcca cattgtgccc ccagagctgc gaggagagga atctccggga 3660 gaacgggtat gagtgtgagt ggcgctataa cagctgtgca cctgcctgtc aagtcacgtg 3720 tcagcaccct gagccactgg cctgccctgt gcagtgtgtg gagggctgcc atgcccactg 3780 ccctccaggg aaaatcctgg atgagctttt gcagacctgc gttgaccctg aagactgtcc 3840 agtgtgtgag gtggctggcc ggcgttttgc ctcaggaaag aaagtcacct tgaatcccag 3900 tgaccctgag cactgccaga tttgccactg tgatgttgtc aacctcacct gtgaagcctg 3960 ccaggagccg ggaggcctgg tggtgcctcc cacagatgcc ccggtgagcc ccaccactct 4020 gtatgtggag gacatctcgg aaccgccgtt gcacgatttc tactgcagca ggctactgga 4080 cctggtcttc ctgctggatg gctcctccag gctgtccgag gctgagtttg aagtgctgaa 4140 ggcctttgtg gtggacatga tggagcggct gcgcatctcc cagaagtggg tccgcgtggc 4200 cgtggtggag taccacgacg gctcccacgc ctacatcggg ctcaaggacc ggaagcgacc 4260 gtcagagctg cggcgcattg ccagccaggt gaagtatgcg ggcagccagg tggcctccac 4320 cagcgaggtc ttgaaataca cactgttcca aatcttcagc aagatcgacc gccctgaagc 4380 ctcccgcatc accctgctcc tgatggccag ccaggagccc caacggatgt cccggaactt 4440 tgtccgctac gtccagggcc tgaagaagaa gaaggtcatt gtgatcccgg tgggcattgg 4500 gccccatgcc aacctcaagc agatccgcct catcgagaag caggcccctg agaacaaggc 4560 cttcgtgctg agcagtgtgg atgagctgga gcagcaaagg gacgagatcg ttagctacct 4620 ctgtgacctt gcccctgaag cccctcctcc tactctgccc cccgacatgg cacaagtcac 4680 tgtgggcccg gggctcttgg gggtttcgac cctggggccc aagaggaact ccatggttct 4740 ggatgtggcg ttcgtcctgg aaggatcgga caaaattggt gaagccgact tcaacaggag 4800 caaggagttc atggaggagg tgattcagcg gatggatgtg ggccaggaca gcatccacgt 4860 cacggtgctg cagtactcct acatggtgac tgtggagtac cccttcagcg aggcacagtc 4920 caaaggggac atcctgcagc gggtgcgaga gatccgctac cagggcggca acaggaccaa 4980 cactgggctg gccctgcggt acctctctga ccacagcttc ttggtcagcc agggtgaccg 5040 ggagcaggcg cccaacctgg tctacatggt caccggaaat cctgcctctg atgagatcaa 5100 gaggctgcct ggagacatcc aggtggtgcc cattggagtg ggccctaatg ccaacgtgca 5160 ggagctggag aggattggct ggcccaatgc ccctatcctc atccaggact ttgagacgct 5220 cccccgagag gctcctgacc tggtgctgca gaggtgctgc tccggagagg ggctgcagat 5280 ccccaccctc tcccctgcac ctgactgcag ccagcccctg gacgtgatcc ttctcctgga 5340 tggctcctcc agtttcccag cttcttattt tgatgaaatg aagagtttcg ccaaggcttt 5400 catttcaaaa gccaatatag ggcctcgtct cactcaggtg tcagtgctgc agtatggaag 5460 catcaccacc attgacgtgc catggaacgt ggtcccggag aaagcccatt tgctgagcct 5520 tgtggacgtc atgcagcggg agggaggccc cagccaaatc ggggatgcct tgggctttgc 5580 tgtgcgatac ttgacttcag aaatgcatgg tgccaggccg ggagcctcaa aggcggtggt 5640 catcctggtc acggacgtct ctgtggattc agtggatgca gcagctgatg ccgccaggtc 5700 caacagagtg acagtgttcc ctattggaat tggagatcgc tacgatgcag cccagctacg 5760 gatcttggca ggcccagcag gcgactccaa cgtggtgaag ctccagcgaa tcgaagacct 5820 ccctaccatg gtcaccttgg gcaattcctt cctccacaaa ctgtgctctg gatttgttag 5880 gatttgcatg gatgaggatg ggaatgagaa gaggcccggg gacgtctgga ccttgccaga 5940 ccagtgccac accgtgactt gccagccaga tggccagacc ttgctgaaga gtcatcgggt 6000 caactgtgac cgggggctga ggccttcgtg ccctaacagc cagtcccctg ttaaagtgga 6060 agagacctgt ggctgccgct ggacctgccc ctgcgtgtgc acaggcagct ccactcggca 6120 catcgtgacc tttgatgggc agaatttcaa gctgactggc agctgttctt atgtcctatt 6180 tcaaaacaag gagcaggacc tggaggtgat tctccataat ggtgcctgca gccctggagc 6240 aaggcagggc tgcatgaaat ccatcgaggt gaagcacagt gccctctccg tcgagctgca 6300 cagtgacatg gaggtgacgg tgaatgggag actggtctct gttccttacg tgggtgggaa 6360 catggaagtc aacgtttatg gtgccatcat gcatgaggtc agattcaatc accttggtca 6420 catcttcaca ttcactccac aaaacaatga gttccaactg cagctcagcc ccaagacttt 6480 tgcttcaaag acgtatggtc tgtgtgggat ctgtgatgag aacggagcca atgacttcat 6540 gctgagggat ggcacagtca ccacagactg gaaaacactt gttcaggaat ggactgtgca 6600 gcggccaggg cagacgtgcc agcccatcct ggaggagcag tgtcttgtcc ccgacagctc 6660 ccactgccag gtcctcctct taccactgtt tgctgaatgc cacaaggtcc tggctccagc 6720 cacattctat gccatctgcc agcaggacag ttgccaccag gagcaagtgt gtgaggtgat 6780 cgcctcttat gcccacctct gtcggaccaa cggggtctgc gttgactgga ggacacctga 6840 tttctgtgct atgtcatgcc caccatctct ggtctacaac cactgtgagc atggctgtcc 6900 ccggcactgt gatggcaacg tgagctcctg tggggaccat ccctccgaag gctgtttctg 6960 ccctccagat aaagtcatgt tggaaggcag ctgtgtccct gaagaggcct gcactcagtg 7020 cattggtgag gatggagtcc agcaccagtt cctggaagcc tgggtcccgg accaccagcc 7080 ctgtcagatc tgcacatgcc tcagcgggcg gaaggtcaac tgcacaacgc agccctgccc 7140 cacggccaaa gctcccacgt gtggcctgtg tgaagtagcc cgcctccgcc agaatgcaga 7200 ccagtgctgc cccgagtatg agtgtgtgtg tgacccagtg agctgtgacc tgcccccagt 7260 gcctcactgt gaacgtggcc tccagcccac actgaccaac cctggcgagt gcagacccaa 7320 cttcacctgc gcctgcagga aggaggagtg caaaagagtg tccccaccct cctgcccccc 7380 gcaccgtttg cccacccttc ggaagaccca gtgctgtgat gagtatgagt gtgcctgcaa 7440 ctgtgtcaac tccacagtga gctgtcccct tgggtacttg gcctcaactg ccaccaatga 7500 ctgtggctgt accacaacca cctgccttcc cgacaaggtg tgtgtccacc gaagcaccat 7560 ctaccctgtg ggccagttct gggaggaggg ctgcgatgtg tgcacctgca ccgacatgga 7620 ggatgccgtg atgggcctcc gcgtggccca gtgctcccag aagccctgtg aggacagctg 7680 tcggtcgggc ttcacttacg ttctgcatga aggcgagtgc tgtggaaggt gcctgccatc 7740 tgcctgtgag gtggtgactg gctcaccgcg gggggactcc cagtcttcct ggaagagtgt 7800 cggctcccag tgggcctccc cggagaaccc ctgcctcatc aatgagtgtg tccgagtgaa 7860 ggaggaggtc tttatacaac aaaggaacgt ctcctgcccc cagctggagg tccctgtctg 7920 cccctcgggc tttcagctga gctgtaagac ctcagcgtgc tgcccaagct gtcgctgtga 7980 gcgcatggag gcctgcatgc tcaatggcac tgtcattggg cccgggaaga ctgtgatgat 8040 cgatgtgtgc acgacctgcc gctgcatggt gcaggtgggg gtcatctctg gattcaagct 8100 ggagtgcagg aagaccacct gcaacccctg ccccctgggt tacaaggaag aaaataacac 8160 aggtgaatgt tgtgggagat gtttgcctac ggcttgcacc attcagctaa gaggaggaca 8220 gatcatgaca ctgaagcgtg atgagacgct ccaggatggc tgtgatactc acttctgcaa 8280 ggtcaatgag agaggagagt acttctggga gaagagggtc acaggctgcc caccctttga 8340 tgaacacaag tgtctggctg agggaggtaa aattatgaaa attccaggca cctgctgtga 8400 cacatgtgag gagcctgagt gcaacgacat cactgccagg ctgcagtatg tcaaggtggg 8460 aagctgtaag tctgaagtag aggtggatat ccactactgc cagggcaaat gtgccagcaa 8520 agccatgtac tccattgaca tcaacgatgt gcaggaccag tgctcctgct gctctccgac 8580 acggacggag cccatgcagg tggccctgca ctgcaccaat ggctctgttg tgtaccatga 8640 ggttctcaat gccatggagt gcaaatgctc ccccaggaag tgcagcaagt gaggctgctg 8700 cagctgcatg ggtgcctgct gctgcctgcc ttggcctgat ggccaggcca gagtgctgcc 8760 agtcctctgc atgttctgct cttgtgccct tctgagccca caataaaggc tgagctctta 8820 tcttgcaaaa ggc 8833 < 210 > 2 < 211 > 2783 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic Polypeptide < 220 > < 221 > MISC _ FEATURE < 223 > prepro - vWF < 400 > 2 Met Ile Pro Ala Arg Phe Ala Gly Val Leu Leu Leu Ile Leu Pro Gly 1 5 10 15 Thr Leu Cys Ala Glu Gly Thr Arg Gly Arg Ser Ser Thr Ala Arg Cys 20 25 30 Ser Leu Phe Gly Ser Asp Phe Val Asn Thr Phe Asp Gly Ser Met Tyr 35 40 45 Ser Phe Ala Gly Tyr Cys Ser Tyr Leu Leu Ala Gly Gly Cys Gln Lys 50 55 60 Arg Ser Phe Ser Ile Ile Gly Asp Phe Gln Asn Gly Lys Arg Val Ser 65 70 75 80 Leu Ser Val Tyr Leu Gly Glu Phe Phe Asp Ile His Leu Phe Val Asn 85 90 95 Gly Thr Val Thr Gln Gly Asp Gln Arg Val Ser Met Pro Tyr Ala Ser 100 105 110 Lys Leu Glu Thr Glu Ala Gly Tyr Tyr Lys Leu Ser Gly Glu Ala Tyr 115 120 125 Gly Phe Val Ala Arg Ile Asp Gly Ser Gly Asn Phe Gln Val Leu Leu 130 135 140 Ser Asp Arg Tyr Phe Asn Lys Thr Cys Gly Leu Cys Gly Asn Phe Asn 145 150 155 160 Ile Phe Ala Glu Asp Asp Phe Met Thr Gln Glu Gly Thr Leu Thr Ser 165 170 175 Asp Pro Tyr Asp Phe Ala Asn Ser Trp Ala Leu Ser Ser Gly Glu Gln 180 185 190 Trp Cys Glu Arg Pro Ser Ser Ser Cys Asn Ile Ser Ser Gly Glu Met 195 200 205 Gln Lys Gly Leu Trp Glu Gln Cys Gln Leu Leu Lys Ser Thr Ser Val 210 215 220 Phe Ala Arg Cys His Pro Leu Val Asp Pro Glu Pro Phe Cys Glu Lys 225 230 235 240 Thr Leu Cys Glu Cys Ala Gly Gly Leu Glu Cys Ala Cys Pro Ala Leu 245 250 255 Leu Glu Tyr Ala Arg Thr Cys Ala Gln Glu Gly Met Val Leu Tyr Gly 260 265 270 Trp Thr Asp His Ser Ala Cys Ser Pro Val Cys Pro Ala Gly Met Glu 275 280 285 Tyr Arg Gln Cys Val Ser Pro Cys Ala Arg Thr Cys Gln Ser Leu His 290 295 300 Ile Asn Glu Met Cys Gln Glu Arg Cys Val Asp Gly Cys Ser Cys Pro 305 310 315 320 Glu Gly Gln Leu Leu Asp Glu Gly Leu Cys Val Glu Ser Thr Glu Cys 325 330 335 Pro Cys Val His Ser Gly Lys Arg Tyr Pro Pro Gly Thr Ser Leu Ser 340 345 350 Arg Asp Cys Asn Thr Cys Ile Cys Arg Asn Ser Gln Trp Ile Cys Ser 355 360 365 Asn Glu Glu Cys Pro Gly Glu Cys Leu Val Thr Gly Gln Ser His Phe 370 375 380 Lys Ser Phe Asp Asn Arg Tyr Phe Thr Phe Ser Gly Ile Cys Gln Tyr 385 390 395 400 Leu Leu Ala Arg Asp Cys Gln Asp His Ser Phe Ser Ile Val Ile Glu 405 410 415 Thr Val Gln Cys Ala Asp Asp Arg Asp Ala Val Cys Thr Arg Ser Val 420 425 430 Thr Val Arg Leu Pro Gly Leu His Asn Ser Leu Val Lys Leu Lys His 435 440 445 Gly Ala Gly Val Ala Met Asp Gly Gln Asp Val Gln Leu Pro Leu Leu 450 455 460 Lys Gly Asp Leu Arg Ile Gln His Thr Val Thr Ala Ser Val Arg Leu 465 470 475 480 Ser Tyr Gly Glu Asp Leu Gln Met Asp Trp Asp Gly Arg Gly Arg Leu 485 490 495 Leu Val Lys Leu Ser Pro Val Tyr Ala Gly Lys Thr Cys Gly Leu Cys 500 505 510 Gly Asn Tyr Asn Gly Asn Gln Gly Asp Asp Phe Leu Thr Pro Ser Gly 515 520 525 Leu Ala Glu Pro Arg Val Glu Asp Phe Gly Asn Ala Trp Lys Leu His 530 535 540 Gly Asp Cys Gln Asp Leu Gln Lys Gln His Ser Asp Pro Cys Ala Leu 545 550 555 560 Asn Pro Arg Met Thr Arg Phe Ser Glu Glu Ala Cys Ala Val Leu Thr 565 570 575 Ser Pro Thr Phe Glu Ala Cys His Arg Ala Val Ser Pro Leu Pro Tyr 580 585 590 Leu Arg Asn Cys Arg Tyr Asp Val Cys Ser Cys Ser Asp Gly Arg Glu 595 600 605 Cys Leu Cys Gly Ser Tyr Ala Ala Ala Cys Ala Gly Arg Gly Val Arg 610 615 620 Val Ala Trp Arg Glu Pro Gly Arg Cys Glu Leu Asn Cys Pro Lys Gly 625 630 635 640 Gln Val Tyr Leu Gln Cys Gly Thr Pro Cys Asn Leu Thr Cys Arg Ser 645 650 655 Leu Ser Tyr Pro Asp Glu Glu Cys Asn Glu Ala Cys Leu Glu Gly Cys 660 665 670 Phe Cys Pro Pro Met Asp Glu Arg Gly Asp Cys Val Pro Lys Ala Gln 675 680 685 Cys Pro Cys Tyr Tyr Asp Gly Glu Ile Phe Gln Pro Glu Asp Ile Phe 690 695 700 Ser Asp His His Thr Met Cys Tyr Cys Glu Asp Gly Phe Met His Cys 705 710 715 720 Thr Met Ser Gly Val Pro Gly Ser Leu Leu Pro Asp Ala Val Leu Ser 725 730 735 Ser Pro Leu Ser His Arg Ser Lys Arg Ser Leu Ser Cys Arg Pro Pro 740 745 750 Met Val Lys Leu Val Cys Pro Ala Asp Asn Leu Arg Ala Glu Gly Leu 755 760 765 Glu Cys Thr Lys Thr Cys Gln Asn Tyr Asp Leu Glu Cys Met Ser Met 770 775 780 Gly Cys Val Ser Gly Cys Leu Cys Pro Pro Gly Met Val Arg His Glu 785 790 795 800 Asn Arg Cys Glu Arg Cys Pro Cys Phe His Gln Gly Lys Glu Tyr Ala 805 810 815 Pro Gly Glu Thr Val Lys Ile Gly Cys Asn Thr Cys Val Cys Arg Asp 820 825 830 Arg Lys Trp Asn Cys Thr Asp His Val Cys Asp Ala Thr Cys Ser Thr 835 840 845 Ile Gly Met Ala His Tyr Leu Thr Phe Asp Gly Leu Lys Tyr Leu Phe 850 855 860 Pro Gly Glu Cys Gln Tyr Val Leu Val Gln Asp Tyr Cys Gly Ser Asn 865 870 875 880 Pro Gly Thr Phe Arg Ile Leu Val Gly Asn Lys Gly Cys Ser His Pro 885 890 895 Ser Val Lys Cys Lys Lys Arg Val Thr Ile Leu Val Glu Gly Gly Glu 900 905 910 Ile Glu Leu Phe Asp Gly Glu Val Asn Val Lys Arg Pro Met Lys Asp 915 920 925 Glu Thr His Phe Glu Val Val Glu Ser Gly Arg Tyr Ile Ile Leu Leu 930 935 940 Leu Gly Lys Ala Leu Ser Val Val Trp Asp Arg His Leu Ser Ile Ser 945 950 955 960 Val Val Leu Lys Gln Thr Tyr Gln Glu Lys Val Cys Gly Leu Cys Gly 965 970 975 Asn Phe Asp Gly Ile Gln Asn Asn Asp Leu Thr Ser Ser Asn Leu Gln 980 985 990 Val Glu Glu Asp Pro Val Asp Phe Gly Asn Ser Trp Lys Val Ser Ser 995 1000 1005 Gln Cys Ala Asp Thr Arg Lys Val Pro Leu Asp Ser Ser Pro Ala 1010 1015 1020 Thr Cys His Asn Asn Ile Met Lys Gln Thr Met Val Asp Ser Ser 1025 1030 1035 Cys Arg Ile Leu Thr Ser Asp Val Phe Gln Asp Cys Asn Lys Leu 1040 1045 1050 Val Asp Pro Glu Pro Tyr Leu Asp Val Cys Ile Tyr Asp Thr Cys 1055 1060 1065 Ser Cys Glu Ser Ile Gly Asp Cys Ala Cys Phe Cys Asp Thr Ile 1070 1075 1080 Ala Ala Tyr Ala His Val Cys Ala Gln His Gly Lys Val Val Thr 1085 1090 1095 Trp Arg Thr Ala Thr Leu Cys Pro Gln Ser Cys Glu Glu Arg Asn 1100 1105 1110 Leu Arg Glu Asn Gly Tyr Glu Cys Glu Trp Arg Tyr Asn Ser Cys 1115 1120 1125 Ala Pro Ala Cys Gln Val Thr Cys Gln His Pro Glu Pro Leu Ala 1130 1135 1140 Cys Pro Val Gln Cys Val Glu Gly Cys His Ala His Cys Pro Pro 1145 1150 1155 Gly Lys Ile Leu Asp Glu Leu Leu Gln Thr Cys Val Asp Pro Glu 1160 1165 1170 Asp Cys Pro Val Cys Glu Val Ala Gly Arg Arg Phe Ala Ser Gly 1175 1180 1185 Lys Lys Val Thr Leu Asn Pro Ser Asp Pro Glu His Cys Gln Ile 1190 1195 1200 Cys His Cys Asp Val Val Asn Leu Thr Cys Glu Ala Cys Gln Glu 1205 1210 1215 Pro Gly Gly Leu Val Val Pro Pro Thr Asp Ala Pro Val Ser Pro 1220 1225 1230 Thr Thr Leu Tyr Val Glu Asp Ile Ser Glu Pro Pro Leu His Asp 1235 1240 1245 Phe Tyr Cys Ser Arg Leu Leu Asp Leu Val Phe Leu Leu Asp Gly 1250 1255 1260 Ser Ser Arg Leu Ser Glu Ala Glu Phe Glu Val Leu Lys Ala Phe 1265 1270 1275 Val Val Asp Met Met Glu Arg Leu Arg Ile Ser Gln Lys Trp Val 1280 1285 1290 Arg Val Ala Val Val Glu Tyr His Asp Gly Ser His Ala Tyr Ile 1295 1300 1305 Gly Leu Lys Asp Arg Lys Arg Pro Ser Glu Leu Arg Arg Ile Ala 1310 1315 1320 Ser Gln Val Lys Tyr Ala Gly Ser Gln Val Ala Ser Thr Ser Glu 1325 1330 1335 Val Leu Lys Tyr Thr Leu Phe Gln Ile Phe Ser Lys Ile Asp Arg 1340 1345 1350 Pro Glu Ala Ser Arg Ile Thr Leu Leu Leu Met Ala Ser Gln Glu 1355 1360 1365 Pro Gln Arg Met Ser Arg Asn Phe Val Arg Tyr Val Gln Gly Leu 1370 1375 1380 Lys Lys Lys Lys Val Ile Val Ile Pro Val Gly Ile Gly Pro His 1385 1390 1395 Ala Asn Leu Lys Gln Ile Arg Leu Ile Glu Lys Gln Ala Pro Glu 1400 1405 1410 Asn Lys Ala Phe Val Leu Ser Ser Val Asp Glu Leu Glu Gln Gln 1415 1420 1425 Arg Asp Glu Ile Val Ser Tyr Leu Cys Asp Leu Ala Pro Glu Ala 1430 1435 1440 Pro Pro Pro Thr Leu Pro Pro Asp Met Ala Gln Val Thr Val Gly 1445 1450 1455 Pro Gly Leu Leu Gly Val Ser Thr Leu Gly Pro Lys Arg Asn Ser 1460 1465 1470 Met Val Leu Asp Val Ala Phe Val Leu Glu Gly Ser Asp Lys Ile 1475 1480 1485 Gly Glu Ala Asp Phe Asn Arg Ser Lys Glu Phe Met Glu Glu Val 1490 1495 1500 Ile Gln Arg Met Asp Val Gly Gln Asp Ser Ile His Val Thr Val 1505 1510 1515 Leu Gln Tyr Ser Tyr Met Val Thr Val Glu Tyr Pro Phe Ser Glu 1520 1525 1530 Ala Gln Ser Lys Gly Asp Ile Leu Gln Arg Val Arg Glu Ile Arg 1535 1540 1545 Tyr Gln Gly Gly Asn Arg Thr Asn Thr Gly Leu Ala Leu Arg Tyr 1550 1555 1560 Leu Ser Asp His Ser Phe Leu Val Ser Gln Gly Asp Arg Glu Gln 1565 1570 1575 Ala Pro Asn Leu Val Tyr Met Val Thr Gly Asn Pro Ala Ser Asp 1580 1585 1590 Glu Ile Lys Arg Leu Pro Gly Asp Ile Gln Val Val Pro Ile Gly 1595 1600 1605 Val Gly Pro Asn Ala Asn Val Gln Glu Leu Glu Arg Ile Gly Trp 1610 1615 1620 Pro Asn Ala Pro Ile Leu Ile Gln Asp Phe Glu Thr Leu Pro Arg 1625 1630 1635 Glu Ala Pro Asp Leu Val Leu Gln Arg Cys Cys Ser Gly Glu Gly 1640 1645 1650 Leu Gln Ile Pro Thr Leu Ser Pro Ala Pro Asp Cys Ser Gln Pro 1655 1660 1665 Leu Asp Val Ile Leu Leu Leu Asp Gly Ser Ser Ser Phe Pro Ala 1670 1675 1680 Ser Tyr Phe Asp Glu Met Lys Ser Phe Ala Lys Ala Phe Ile Ser 1685 1690 1695 Lys Ala Asn Ile Gly Pro Arg Leu Thr Gln Val Ser Val Leu Gln 1700 1705 1710 Tyr Gly Ser Ile Thr Thr Ile Asp Val Pro Trp Asn Val Val Pro 1715 1720 1725 Glu Lys Ala His Leu Leu Ser Leu Val Asp Val Met Gln Arg Glu 1730 1735 1740 Gly Gly Pro Ser Gln Ile Gly Asp Ala Leu Gly Phe Ala Val Arg 1745 1750 1755 Tyr Leu Thr Ser Glu Met His Gly Ala Arg Pro Gly Ala Ser Lys 1760 1765 1770 Ala Val Val Ile Leu Val Thr Asp Val Ser Val Asp Ser Val Asp 1775 1780 1785 Ala Ala Ala Asp Ala Ala Arg Ser Asn Arg Val Thr Val Phe Pro 1790 1795 1800 Ile Gly Ile Gly Asp Arg Tyr Asp Ala Ala Gln Leu Arg Ile Leu 1805 1810 1815 Ala Gly Pro Ala Gly Asp Ser Asn Val Val Lys Leu Gln Arg Ile 1820 1825 1830 Glu Asp Leu Pro Thr Met Val Thr Leu Gly Asn Ser Phe Leu His 1835 1840 1845 Lys Leu Cys Ser Gly Phe Val Arg Ile Cys Met Asp Glu Asp Gly 1850 1855 1860 Asn Glu Lys Arg Pro Gly Asp Val Trp Thr Leu Pro Asp Gln Cys 1865 1870 1875 His Thr Val Thr Cys Gln Pro Asp Gly Gln Thr Leu Leu Lys Ser 1880 1885 1890 His Arg Val Asn Cys Asp Arg Gly Leu Arg Pro Ser Cys Pro Asn 1895 1900 1905 Ser Gln Ser Pro Val Lys Val Glu Glu Thr Cys Gly Cys Arg Trp 1910 1915 1920 Thr Cys Pro Cys Val Cys Thr Gly Ser Ser Thr Arg His Ile Val 1925 1930 1935 Thr Phe Asp Gly Gln Asn Phe Lys Leu Thr Gly Ser Cys Ser Tyr 1940 1945 1950 Val Leu Phe Gln Asn Lys Glu Gln Asp Leu Glu Val Ile Leu His 1955 1960 1965 Asn Gly Ala Cys Ser Pro Gly Ala Arg Gln Gly Cys Met Lys Ser 1970 1975 1980 Ile Glu Val Lys His Ser Ala Leu Ser Val Glu Leu His Ser Asp 1985 1990 1995 Met Glu Val Thr Val Asn Gly Arg Leu Val Ser Val Pro Tyr Val 2000 2005 2010 Gly Gly Asn Met Glu Val Asn Val Tyr Gly Ala Ile Met His Glu 2015 2020 2025 Val Arg Phe Asn His Leu Gly His Ile Phe Thr Phe Thr Pro Gln 2030 2035 2040 Asn Asn Glu Phe Gln Leu Gln Leu Ser Pro Lys Thr Phe Ala Ser 2045 2050 2055 Lys Thr Tyr Gly Leu Cys Gly Ile Cys Asp Glu Asn Gly Ala Asn 2060 2065 2070 Asp Phe Met Leu Arg Asp Gly Thr Val Thr Thr Asp Trp Lys Thr 2075 2080 2085 Leu Val Gln Glu Trp Thr Val Gln Arg Pro Gly Gln Thr Cys Gln 2090 2095 2100 Pro Glu Gln Cys Leu Val Pro Asp Ser Ser His Cys Gln Val Leu 2105 2110 2115 Leu Leu Pro Leu Phe Ala Glu Cys His Lys Val Leu Ala Pro Ala 2120 2125 2130 Thr Phe Tyr Ala Ile Cys Gln Gln Asp Ser Cys His Gln Glu Gln 2135 2140 2145 Val Cys Glu Val Ile Ala Ser Tyr Ala His Leu Cys Arg Thr Asn 2150 2155 2160 Gly Val Cys Val Asp Trp Arg Thr Pro Asp Phe Cys Ala Met Ser 2165 2170 2175 Cys Pro Pro Ser Leu Val Tyr Asn His Cys Glu His Gly Cys Pro 2180 2185 2190 Arg His Cys Asp Gly Asn Val Ser Ser Cys Gly Asp His Pro Ser 2195 2200 2205 Glu Gly Cys Phe Cys Pro Pro Asp Lys Val Met Leu Glu Gly Ser 2210 2215 2220 Cys Val Pro Glu Glu Ala Cys Thr Gln Cys Ile Gly Glu Asp Gly 2225 2230 2235 Val Gln His Gln Phe Leu Glu Ala Trp Val Pro Asp His Gln Pro 2240 2245 2250 Cys Gln Ile Cys Thr Cys Leu Ser Gly Arg Lys Val Asn Cys Thr 2255 2260 2265 Thr Gln Pro Cys Pro Thr Ala Lys Ala Pro Thr Cys Gly Leu Cys 2270 2275 2280 Glu Val Ala Arg Leu Arg Gln Asn Ala Asp Gln Cys Cys Pro Glu 2285 2290 2295 Tyr Glu Cys Val Cys Asp Pro Val Ser Cys Asp Leu Pro Pro Val 2300 2305 2310 Pro His Cys Glu Arg Gly Leu Gln Pro Thr Leu Thr Asn Pro Gly 2315 2320 2325 Glu Cys Arg Pro Asn Phe Thr Cys Ala Cys Arg Lys Glu Glu Cys 2330 2335 2340 Lys Arg Val Ser Pro Pro Ser Cys Pro Pro His Arg Leu Pro Thr 2345 2350 2355 Leu Arg Lys Thr Gln Cys Cys Asp Glu Tyr Glu Cys Ala Cys Asn 2360 2365 2370 Cys Val Asn Ser Thr Val Ser Cys Pro Leu Gly Tyr Leu Ala Ser 2375 2380 2385 Thr Ala Thr Asn Asp Cys Gly Cys Thr Thr Thr Thr Cys Leu Pro 2390 2395 2400 Asp Lys Val Cys Val His Arg Ser Thr Ile Tyr Pro Val Gly Gln 2405 2410 2415 Phe Trp Glu Glu Gly Cys Asp Val Cys Thr Cys Thr Asp Met Glu 2420 2425 2430 Asp Ala Val Met Gly Leu Arg Val Ala Gln Cys Ser Gln Lys Pro 2435 2440 2445 Cys Glu Asp Ser Cys Arg Ser Gly Phe Thr Tyr Val Leu His Glu 2450 2455 2460 Gly Glu Cys Cys Gly Arg Cys Leu Pro Ser Ala Cys Glu Val Val 2465 2470 2475 Thr Gly Ser Pro Arg Gly Asp Ser Gln Ser Ser Trp Lys Ser Val 2480 2485 2490 Gly Ser Gln Trp Glu Asn Pro Cys Leu Ile Asn Glu Cys Val Arg 2495 2500 2505 Val Lys Glu Glu Val Phe Ile Gln Gln Arg Asn Val Ser Cys Pro 2510 2515 2520 Gln Leu Glu Val Pro Val Cys Pro Ser Gly Phe Gln Leu Ser Cys 2525 2530 2535 Lys Thr Ser Ala Cys Cys Pro Ser Cys Arg Cys Glu Arg Met Glu 2540 2545 2550 Ala Cys Met Leu Asn Gly Thr Val Ile Gly Pro Gly Lys Thr Val 2555 2560 2565 Met Ile Asp Val Cys Thr Thr Cys Arg Cys Met Val Gln Val Gly 2570 2575 2580 Val Ile Ser Gly Phe Lys Leu Glu Cys Arg Lys Thr Thr Cys Asn 2585 2590 2595 Pro Cys Pro Leu Gly Tyr Lys Glu Glu Asn Asn Thr Gly Glu Cys 2600 2605 2610 Cys Gly Arg Cys Leu Pro Thr Ala Cys Thr Ile Gln Leu Arg Gly 2615 2620 2625 Gly Gln Ile Met Thr Leu Lys Arg Asp Glu Thr Leu Gln Asp Gly 2630 2635 2640 Cys Asp Thr His Phe Cys Lys Val Asn Glu Arg Gly Glu Tyr Phe 2645 2650 2655 Trp Glu Lys Arg Val Thr Gly Cys Pro Pro Phe Asp Glu His Lys 2660 2665 2670 Cys Leu Ala Glu Gly Gly Lys Ile Met Lys Ile Pro Gly Thr Cys 2675 2680 2685 Cys Asp Thr Cys Glu Glu Pro Glu Cys Asn Asp Ile Thr Ala Arg 2690 2695 2700 Leu Gln Tyr Val Lys Val Gly Ser Cys Lys Ser Glu Val Glu Val 2705 2710 2715 Asp Ile His Tyr Cys Gln Gly Lys Cys Ala Ser Lys Ala Met Tyr 2720 2725 2730 Ser Ile Asp Ile Asn Asp Val Gln Asp Gln Cys Ser Cys Cys Ser 2735 2740 2745 Pro Thr Arg Thr Glu Pro Met Gln His Cys Thr Asn Gly Ser Val 2750 2755 2760 Val Tyr His Glu Val Leu Asn Ala Met Glu Cys Lys Cys Ser Pro 2765 2770 2775 Arg Lys Cys Ser Lys 2780 < 210 > 3 < 211 > 2050 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Synthetic Polypeptide < 220 > < 221 > MISC _ FEATURE < 223 > mature vWF < 400 > 3 Ser Leu Ser Cys Arg Pro Pro Met Val Lys Leu Val Cys Pro Ala Asp 1 5 10 15 Asn Leu Arg Ala Glu Gly Leu Glu Cys Thr Lys Thr Cys Gln Asn Tyr 20 25 30 Asp Leu Glu Cys Met Ser Met Gly Cys Val Ser Gly Cys Leu Cys Pro 35 40 45 Pro Gly Met Val Arg His Glu Asn Arg Cys Val Ala Leu Glu Arg Cys 50 55 60 Pro Cys Phe His Gln Gly Lys Glu Tyr Ala Pro Gly Glu Thr Val Lys 65 70 75 80 Ile Gly Cys Asn Thr Cys Val Cys Arg Asp Arg Lys Trp Asn Cys Thr 85 90 95 Asp His Val Cys Asp Ala Thr Cys Ser Thr Ile Gly Met Ala His Tyr 100 105 110 Leu Thr Phe Asp Gly Leu Lys Tyr Leu Phe Pro Gly Glu Cys Gln Tyr 115 120 125 Val Leu Val Gln Asp Tyr Cys Gly Ser Asn Pro Gly Thr Phe Arg Ile 130 135 140 Leu Val Gly Asn Lys Gly Cys Ser His Pro Ser Val Lys Cys Lys Lys 145 150 155 160 Arg Val Thr Ile Leu Val Glu Gly Gly Glu Ile Glu Leu Phe Asp Gly 165 170 175 Glu Val Asn Val Lys Arg Pro Met Lys Asp Glu Thr His Phe Glu Val 180 185 190 Val Glu Ser Gly Arg Tyr Ile Ile Leu Leu Leu Gly Lys Ala Leu Ser 195 200 205 Val Val Trp Asp Arg His Leu Ser Ile Ser Val Val Leu Lys Gln Thr 210 215 220 Tyr Gln Glu Lys Val Cys Gly Leu Cys Gly Asn Phe Asp Gly Ile Gln 225 230 235 240 Asn Asn Asp Leu Thr Ser Ser Asn Leu Gln Val Glu Glu Asp Pro Val 245 250 255 Asp Phe Gly Asn Ser Trp Lys Val Ser Ser Gln Cys Ala Asp Thr Arg 260 265 270 Lys Val Pro Leu Asp Ser Ser Pro Ala Thr Cys His Asn Asn Ile Met 275 280 285 Lys Gln Thr Met Val Asp Ser Ser Cys Arg Ile Leu Thr Ser Asp Val 290 295 300 Phe Gln Asp Cys Asn Lys Leu Val Asp Pro Glu Pro Tyr Leu Asp Val 305 310 315 320 Cys Ile Tyr Asp Thr Cys Ser Cys Glu Ser Ile Gly Asp Cys Ala Cys 325 330 335 Phe Cys Asp Thr Ile Ala Ala Tyr Ala His Val Cys Ala Gln His Gly 340 345 350 Lys Val Val Thr Trp Arg Thr Ala Thr Leu Cys Pro Gln Ser Cys Glu 355 360 365 Glu Arg Asn Leu Arg Glu Asn Gly Tyr Glu Cys Glu Trp Arg Tyr Asn 370 375 380 Ser Cys Ala Pro Ala Cys Gln Val Thr Cys Gln His Pro Glu Pro Leu 385 390 395 400 Ala Cys Pro Val Gln Cys Val Glu Gly Cys His Ala His Cys Pro Pro 405 410 415 Gly Lys Ile Leu Asp Glu Leu Leu Gln Thr Cys Val Asp Pro Glu Asp 420 425 430 Cys Pro Val Cys Glu Val Ala Gly Arg Arg Phe Ala Ser Gly Lys Lys 435 440 445 Val Thr Leu Asn Pro Ser Asp Pro Glu His Cys Gln Ile Cys His Cys 450 455 460 Asp Val Val Asn Leu Thr Cys Glu Ala Cys Gln Glu Pro Gly Gly Leu 465 470 475 480 Val Val Pro Pro Thr Asp Ala Pro Val Ser Pro Thr Thr Leu Tyr Val 485 490 495 Glu Asp Ile Ser Glu Pro Pro Leu His Asp Phe Tyr Cys Ser Arg Leu 500 505 510 Leu Asp Leu Val Phe Leu Leu Asp Gly Ser Ser Arg Leu Ser Glu Ala 515 520 525 Glu Phe Glu Val Leu Lys Ala Phe Val Val Asp Met Met Glu Arg Leu 530 535 540 Arg Ile Ser Gln Lys Trp Val Arg Val Ala Val Val Glu Tyr His Asp 545 550 555 560 Gly Ser His Ala Tyr Ile Gly Leu Lys Asp Arg Lys Arg Pro Ser Glu 565 570 575 Leu Arg Arg Ile Ala Ser Gln Val Lys Tyr Ala Gly Ser Gln Val Ala 580 585 590 Ser Thr Ser Glu Val Leu Lys Tyr Thr Leu Phe Gln Ile Phe Ser Lys 595 600 605 Ile Asp Arg Pro Glu Ala Ser Arg Ile Thr Leu Leu Leu Met Ala Ser 610 615 620 Gln Glu Pro Gln Arg Met Ser Arg Asn Phe Val Arg Tyr Val Gln Gly 625 630 635 640 Leu Lys Lys Lys Lys Val Ile Val Ile Pro Val Gly Ile Gly Pro His 645 650 655 Ala Asn Leu Lys Gln Ile Arg Leu Ile Glu Lys Gln Ala Pro Glu Asn 660 665 670 Lys Ala Phe Val Leu Ser Ser Val Asp Glu Leu Glu Gln Gln Arg Asp 675 680 685 Glu Ile Val Ser Tyr Leu Cys Asp Leu Ala Pro Glu Ala Pro Pro Pro 690 695 700 Thr Leu Pro Pro Asp Met Ala Gln Val Thr Val Gly Pro Gly Leu Leu 705 710 715 720 Gly Val Ser Thr Leu Gly Pro Lys Arg Asn Ser Met Val Leu Asp Val 725 730 735 Ala Phe Val Leu Glu Gly Ser Asp Lys Ile Gly Glu Ala Asp Phe Asn 740 745 750 Arg Ser Lys Glu Phe Met Glu Glu Val Ile Gln Arg Met Asp Val Gly 755 760 765 Gln Asp Ser Ile His Val Thr Val Leu Gln Tyr Ser Tyr Met Val Thr 770 775 780 Val Glu Tyr Pro Phe Ser Glu Ala Gln Ser Lys Gly Asp Ile Leu Gln 785 790 795 800 Arg Val Arg Glu Ile Arg Tyr Gln Gly Gly Asn Arg Thr Asn Thr Gly 805 810 815 Leu Ala Leu Arg Tyr Leu Ser Asp His Ser Phe Leu Val Ser Gln Gly 820 825 830 Asp Arg Glu Gln Ala Pro Asn Leu Val Tyr Met Val Thr Gly Asn Pro 835 840 845 Ala Ser Asp Glu Ile Lys Arg Leu Pro Gly Asp Ile Gln Val Val Pro 850 855 860 Ile Gly Val Gly Pro Asn Ala Asn Val Gln Glu Leu Glu Arg Ile Gly 865 870 875 880 Trp Pro Asn Ala Pro Ile Leu Ile Gln Asp Phe Glu Thr Leu Pro Arg 885 890 895 Glu Ala Pro Asp Leu Val Leu Gln Arg Cys Cys Ser Gly Glu Gly Leu 900 905 910 Gln Ile Pro Thr Leu Ser Pro Ala Pro Asp Cys Ser Gln Pro Leu Asp 915 920 925 Val Ile Leu Leu Leu Asp Gly Ser Ser Ser Phe Pro Ala Ser Tyr Phe 930 935 940 Asp Glu Met Lys Ser Phe Ala Lys Ala Phe Ile Ser Lys Ala Asn Ile 945 950 955 960 Gly Pro Arg Leu Thr Gln Val Ser Val Leu Gln Tyr Gly Ser Ile Thr 965 970 975 Thr Ile Asp Val Pro Trp Asn Val Val Pro Glu Lys Ala His Leu Leu 980 985 990 Ser Leu Val Asp Val Met Gln Arg Glu Gly Gly Pro Ser Gln Ile Gly 995 1000 1005 Asp Ala Leu Gly Phe Ala Val Arg Tyr Leu Thr Ser Glu Met His 1010 1015 1020 Gly Ala Arg Pro Gly Ala Ser Lys Ala Val Val Ile Leu Val Thr 1025 1030 1035 Asp Val Ser Val Asp Ser Val Asp Ala Ala Ala Asp Ala Ala Arg 1040 1045 1050 Ser Asn Arg Val Thr Val Phe Pro Ile Gly Ile Gly Asp Arg Tyr 1055 1060 1065 Asp Ala Ala Gln Leu Arg Ile Leu Ala Gly Pro Ala Gly Asp Ser 1070 1075 1080 Asn Val Val Lys Leu Gln Arg Ile Glu Asp Leu Pro Thr Met Val 1085 1090 1095 Thr Leu Gly Asn Ser Phe Leu His Lys Leu Cys Ser Gly Phe Val 1100 1105 1110 Arg Ile Cys Met Asp Glu Asp Gly Asn Glu Lys Arg Pro Gly Asp 1115 1120 1125 Val Trp Thr Leu Pro Asp Gln Cys His Thr Val Thr Cys Gln Pro 1130 1135 1140 Asp Gly Gln Thr Leu Leu Lys Ser His Arg Val Asn Cys Asp Arg 1145 1150 1155 Gly Leu Arg Pro Ser Cys Pro Asn Ser Gln Ser Pro Val Lys Val 1160 1165 1170 Glu Glu Thr Cys Gly Cys Arg Trp Thr Cys Pro Cys Val Cys Thr 1175 1180 1185 Gly Ser Ser Thr Arg His Ile Val Thr Phe Asp Gly Gln Asn Phe 1190 1195 1200 Lys Leu Thr Gly Ser Cys Ser Tyr Val Leu Phe Gln Asn Lys Glu 1205 1210 1215 Gln Asp Leu Glu Val Ile Leu His Asn Gly Ala Cys Ser Pro Gly 1220 1225 1230 Ala Arg Gln Gly Cys Met Lys Ser Ile Glu Val Lys His Ser Ala 1235 1240 1245 Leu Ser Val Glu Leu His Ser Asp Met Glu Val Thr Val Asn Gly 1250 1255 1260 Arg Leu Val Ser Val Pro Tyr Val Gly Gly Asn Met Glu Val Asn 1265 1270 1275 Val Tyr Gly Ala Ile Met His Glu Val Arg Phe Asn His Leu Gly 1280 1285 1290 His Ile Phe Thr Phe Thr Pro Gln Asn Asn Glu Phe Gln Leu Gln 1295 1300 1305 Leu Ser Pro Lys Thr Phe Ala Ser Lys Thr Tyr Gly Leu Cys Gly 1310 1315 1320 Ile Cys Asp Glu Asn Gly Ala Asn Asp Phe Met Leu Arg Asp Gly 1325 1330 1335 Thr Val Thr Thr Asp Trp Lys Thr Leu Val Gln Glu Trp Thr Val 1340 1345 1350 Gln Arg Pro Gly Gln Thr Cys Gln Pro Ile Leu Glu Glu Gln Cys 1355 1360 1365 Leu Val Pro Asp Ser Ser His Cys Gln Val Leu Leu Leu Pro Leu 1370 1375 1380 Phe Ala Glu Cys His Lys Val Leu Ala Pro Ala Thr Phe Tyr Ala 1385 1390 1395 Ile Cys Gln Gln Asp Ser Cys His Gln Glu Gln Val Cys Glu Val 1400 1405 1410 Ile Ala Ser Tyr Ala His Leu Cys Arg Thr Asn Gly Val Cys Val 1415 1420 1425 Asp Trp Arg Thr Pro Asp Phe Cys Ala Met Ser Cys Pro Pro Ser 1430 1435 1440 Leu Val Tyr Asn His Cys Glu His Gly Cys Pro Arg His Cys Asp 1445 1450 1455 Gly Asn Val Ser Ser Cys Gly Asp His Pro Ser Glu Gly Cys Phe 1460 1465 1470 Cys Pro Pro Asp Lys Val Met Leu Glu Gly Ser Cys Val Pro Glu 1475 1480 1485 Glu Ala Cys Thr Gln Cys Ile Gly Glu Asp Gly Val Gln His Gln 1490 1495 1500 Phe Leu Glu Ala Trp Val Pro Asp His Gln Pro Cys Gln Ile Cys 1505 1510 1515 Thr Cys Leu Ser Gly Arg Lys Val Asn Cys Thr Thr Gln Pro Cys 1520 1525 1530 Pro Thr Ala Lys Ala Pro Thr Cys Gly Leu Cys Glu Val Ala Arg 1535 1540 1545 Leu Arg Gln Asn Ala Asp Gln Cys Cys Pro Glu Tyr Glu Cys Val 1550 1555 1560 Cys Asp Pro Val Ser Cys Asp Leu Pro Pro Val Pro His Cys Glu 1565 1570 1575 Arg Gly Leu Gln Pro Thr Leu Thr Asn Pro Gly Glu Cys Arg Pro 1580 1585 1590 Asn Phe Thr Cys Ala Cys Arg Lys Glu Glu Cys Lys Arg Val Ser 1595 1600 1605 Pro Pro Ser Cys Pro Pro His Arg Leu Pro Thr Leu Arg Lys Thr 1610 1615 1620 Gln Cys Cys Asp Glu Tyr Glu Cys Ala Cys Asn Cys Val Asn Ser 1625 1630 1635 Thr Val Ser Cys Pro Leu Gly Tyr Leu Ala Ser Thr Ala Thr Asn 1640 1645 1650 Asp Cys Gly Cys Thr Thr Thr Thr Cys Leu Pro Asp Lys Val Cys 1655 1660 1665 Val His Arg Ser Thr Ile Tyr Pro Val Gly Gln Phe Trp Glu Glu 1670 1675 1680 Gly Cys Asp Val Cys Thr Cys Thr Asp Met Glu Asp Ala Val Met 1685 1690 1695 Gly Leu Arg Val Ala Gln Cys Ser Gln Lys Pro Cys Glu Asp Ser 1700 1705 1710 Cys Arg Ser Gly Phe Thr Tyr Val Leu His Glu Gly Glu Cys Cys 1715 1720 1725 Gly Arg Cys Leu Pro Ser Ala Cys Glu Val Val Thr Gly Ser Pro 1730 1735 1740 Arg Gly Asp Ser Gln Ser Ser Trp Lys Ser Val Gly Ser Gln Trp 1745 1750 1755 Ala Ser Pro Glu Asn Pro Cys Leu Ile Asn Glu Cys Val Arg Val 1760 1765 1770 Lys Glu Glu Val Phe Ile Gln Gln Arg Asn Val Ser Cys Pro Gln 1775 1780 1785 Leu Glu Val Pro Val Cys Pro Ser Gly Phe Gln Leu Ser Cys Lys 1790 1795 1800 Thr Ser Ala Cys Cys Pro Ser Cys Arg Cys Glu Arg Met Glu Ala 1805 1810 1815 Cys Met Leu Asn Gly Thr Val Ile Gly Pro Gly Lys Thr Val Met 1820 1825 1830 Ile Asp Val Cys Thr Thr Cys Arg Cys Met Val Gln Val Gly Val 1835 1840 1845 Ile Ser Gly Phe Lys Leu Glu Cys Arg Lys Thr Thr Cys Asn Pro 1850 1855 1860 Cys Pro Leu Gly Tyr Lys Glu Glu Asn Asn Thr Gly Glu Cys Cys 1865 1870 1875 Gly Arg Cys Leu Pro Thr Ala Cys Thr Ile Gln Leu Arg Gly Gly 1880 1885 1890 Gln Ile Met Thr Leu Lys Arg Asp Glu Thr Leu Gln Asp Gly Cys 1895 1900 1905 Asp Thr His Phe Cys Lys Val Asn Glu Arg Gly Glu Tyr Phe Trp 1910 1915 1920 Glu Lys Arg Val Thr Gly Cys Pro Pro Phe Asp Glu His Lys Cys 1925 1930 1935 Leu Ala Glu Gly Gly Lys Ile Met Lys Ile Pro Gly Thr Cys Cys 1940 1945 1950 Asp Thr Cys Glu Glu Pro Glu Cys Asn Asp Ile Thr Ala Arg Leu 1955 1960 1965 Gln Tyr Val Lys Val Gly Ser Cys Lys Ser Glu Val Glu Val Asp 1970 1975 1980 Ile His Tyr Cys Gln Gly Lys Cys Ala Ser Lys Ala Met Tyr Ser 1985 1990 1995 Ile Asp Ile Asn Asp Val Gln Asp Gln Cys Ser Cys Cys Ser Pro 2000 2005 2010 Thr Arg Thr Glu Pro Met Gln Val Ala Leu His Cys Thr Asn Gly 2015 2020 2025 Ser Val Val Tyr His Glu Val Leu Asn Ala Met Glu Cys Lys Cys 2030 2035 2040 Ser Pro Arg Lys Cys Ser Lys 2045 2050