SEQUENCE LISTING < 110 > DSM IP Assets B . V < 120 > Mutant Type II Beta - Lactam Acylases < 130 > 24988 WO < 160 > 3 < 170 > PatentIn version 3 . 3 < 210 > 1 < 211 > 3141 < 212 > DNA < 213 > Pseudomonas sp . SE 83 < 220 > < 221 > CDS < 222 > ( 562 ) . .( 2886 ) < 400 > 1 aagcttgcga tcgcggacgc cgctgccgcc ctgaagaaga ccgcctacaa gggcgagccg 60 gtggtcttcc tgacggtcgc cgggtcgatc tcgcagacgg ccggcgcggt tctggccagc 120 aatatgcgca aggccggctt caccgtggac gagcaggtga tggactgggg cacggtgctc 180 gcacgccggg ccaagaagga cggttggagc gtcttcccgg tctacgccaa cggcatcgac 240 atgatgtcgc cgctgacgca tttctacatc ggcaacaact gcgcgaacta tgccggctgg 300 agctgcgacg ccgtcatcac ccaaaagctc gccgcctatg ccaaggcgcc tgatccggcc 360 acccgcaagc gcatcgcggc cgagatcagg tcgaggccta taaggacacg ccctccgtga 420 tgtggggcca gttcagccgg ccggccggat accgcctgcg cctcaaagac atcgtccagt 480 ccagcttccg atcttctggc agctcacgct cgacgcgtga gctcgtccag atcccgataa 540 gcaacgaggt ccagacagag a atg acg atg gcg gcc aag acc gat cgc gag 591 Met Thr Met Ala Ala Lys Thr Asp Arg Glu 1 5 10 gcc ctg cag gcg gcg ctg ccg ccg ctt tcc ggc agc ctc tcc att ccc 639 Ala Leu Gln Ala Ala Leu Pro Pro Leu Ser Gly Ser Leu Ser Ile Pro 15 20 25 gga ttg agc gcg ccg gtc cgt gtc cag cgc gat ggc tgg ggc atc ccg 687 Gly Leu Ser Ala Pro Val Arg Val Gln Arg Asp Gly Trp Gly Ile Pro 30 35 40 cat atc aag gcc tcg ggc gag gcc gat gcc tat cgc gcg ctg ggc ttc 735 His Ile Lys Ala Ser Gly Glu Ala Asp Ala Tyr Arg Ala Leu Gly Phe 45 50 55 gtc cat gcg cag gac cgc ctt ttc cag atg gaa ctg acg cgc cgc aag 783 Val His Ala Gln Asp Arg Leu Phe Gln Met Glu Leu Thr Arg Arg Lys 60 65 70 gcg ctg ggt cgc gcg gcc gaa tgg ctg ggc gcc gag gca gcc gag gcc 831 Ala Leu Gly Arg Ala Ala Glu Trp Leu Gly Ala Glu Ala Ala Glu Ala 75 80 85 90 gat atc ttg gtg cgc cgg ctc ggc atg gaa aaa gtc tgc cgg cgc gat 879 Asp Ile Leu Val Arg Arg Leu Gly Met Glu Lys Val Cys Arg Arg Asp 95 100 105 ttc gag gcc ctg ggt gcc gag gcg aag gac atg ctg cgg gcc tat gtc 927 Phe Glu Ala Leu Gly Ala Glu Ala Lys Asp Met Leu Arg Ala Tyr Val 110 115 120 gcc ggc gtg aac gcg ttc ctg gct tcc ggt gct cct ttg ccc atc gaa 975 Ala Gly Val Asn Ala Phe Leu Ala Ser Gly Ala Pro Leu Pro Ile Glu 125 130 135 tat ggc ctg ctc ggc gcc gaa ccg gag ccc tgg gaa ccc tgg cac agc 1023 Tyr Gly Leu Leu Gly Ala Glu Pro Glu Pro Trp Glu Pro Trp His Ser 140 145 150 atc gcc gtg atg cgg cgg ctg ggg ctc ctg atg ggc tcc gtc tgg ttc 1071 Ile Ala Val Met Arg Arg Leu Gly Leu Leu Met Gly Ser Val Trp Phe 155 160 165 170 aag ctc tgg cgg atg ctg gcg ctg ccg gtg gtc gga gcc gcg aat gcg 1119 Lys Leu Trp Arg Met Leu Ala Leu Pro Val Val Gly Ala Ala Asn Ala 175 180 185 ctg aag ctg cgc tat gac gat ggc ggc caa gac ctg ctc tgc atc ccg 1167 Leu Lys Leu Arg Tyr Asp Asp Gly Gly Gln Asp Leu Leu Cys Ile Pro 190 195 200 ccg ggt gtc gag gcc gag cgg ctc gaa gcg gat ctc gcg gcg ctg agg 1215 Pro Gly Val Glu Ala Glu Arg Leu Glu Ala Asp Leu Ala Ala Leu Arg 205 210 215 ccc gcg gtt gat gcc ctg ctg aaa gcg atg ggc ggc gac gcc tcc gat 1263 Pro Ala Val Asp Ala Leu Leu Lys Ala Met Gly Gly Asp Ala Ser Asp 220 225 230 gcg gcc ggc ggc ggc agc aac aac tgg gcg gtc gcg ccg ggc cgc acg 1311 Ala Ala Gly Gly Gly Ser Asn Asn Trp Ala Val Ala Pro Gly Arg Thr 235 240 245 250 gcg acg ggc cgg ccc atc ctc gcg ggc gat ccg cat cgc gtc ttc gaa 1359 Ala Thr Gly Arg Pro Ile Leu Ala Gly Asp Pro His Arg Val Phe Glu 255 260 265 atc ccc ggc atg tat gcg cag cat cac ctg gcc tgc gat cgg ttc gac 1407 Ile Pro Gly Met Tyr Ala Gln His His Leu Ala Cys Asp Arg Phe Asp 270 275 280 atg atc ggt ctg acc gtg ccg ggt gtg ccg ggc ttc ccg cat ttc gcg 1455 Met Ile Gly Leu Thr Val Pro Gly Val Pro Gly Phe Pro His Phe Ala 285 290 295 cat aac ggc aag gtc gcc tac tgc gtc acc cat gcc ttc atg gac att 1503 His Asn Gly Lys Val Ala Tyr Cys Val Thr His Ala Phe Met Asp Ile 300 305 310 cac gat ctc tat ctc gag caa ttc gcg gag gac ggg cgc acg gcg cgg 1551 His Asp Leu Tyr Leu Glu Gln Phe Ala Glu Asp Gly Arg Thr Ala Arg 315 320 325 330 ttc ggc aac gag ttc gag ccc gta gcc tgg cgc cga gac cgt atc gcg 1599 Phe Gly Asn Glu Phe Glu Pro Val Ala Trp Arg Arg Asp Arg Ile Ala 335 340 345 gtc cgg ggt ggc gcc gat cgc gaa ttc gat atc gtc gag acg cgc cat 1647 Val Arg Gly Gly Ala Asp Arg Glu Phe Asp Ile Val Glu Thr Arg His 350 355 360 ggc ccc gtc atc gcg ggc gat ccg ctc gag gga gca gcg ctc acg ctg 1695 Gly Pro Val Ile Ala Gly Asp Pro Leu Glu Gly Ala Ala Leu Thr Leu 365 370 375 cgc tcg gtc cag ttc gcc gag acc gac ctt tcc ttc gat tgc ctg acg 1743 Arg Ser Val Gln Phe Ala Glu Thr Asp Leu Ser Phe Asp Cys Leu Thr 380 385 390 cgg atg ccg ggc gca tcg acc gtg gcg cag ctt tac gac gcg acg cgc 1791 Arg Met Pro Gly Ala Ser Thr Val Ala Gln Leu Tyr Asp Ala Thr Arg 395 400 405 410 ggc tgg ggc ctg atc gac cat aat ctc gtc gcc ggg gat gtc gcg ggc 1839 Gly Trp Gly Leu Ile Asp His Asn Leu Val Ala Gly Asp Val Ala Gly 415 420 425 tcg atc ggc cat ctg gtc cgc gcc cgc gtc ccg tcc cgc ccg cgc gag 1887 Ser Ile Gly His Leu Val Arg Ala Arg Val Pro Ser Arg Pro Arg Glu 430 435 440 aac ggc tgg ctg ccg gtg ccg ggc tgg tcc ggc gag cat gaa tgg cgc 1935 Asn Gly Trp Leu Pro Val Pro Gly Trp Ser Gly Glu His Glu Trp Arg 445 450 455 ggc tgg att ccg cac gag gcg atg ccg cgc gtc atc gat ccg ccg ggc 1983 Gly Trp Ile Pro His Glu Ala Met Pro Arg Val Ile Asp Pro Pro Gly 460 465 470 ggc ctc atc gtc acg gcg aac aac cgc gtc gtg gcc gat gat cat ccc 2031 Gly Leu Ile Val Thr Ala Asn Asn Arg Val Val Ala Asp Asp His Pro 475 480 485 490 gat tat ctc tgt acc gat tgc cat ccg ccc tac cgc gcc gaa cgg atc 2079 Asp Tyr Leu Cys Thr Asp Cys His Pro Pro Tyr Arg Ala Glu Arg Ile 495 500 505 atg gag cgc ctg gtc gcc agt ccg gct ttc gcc gtc gac gat gcg gcc 2127 Met Glu Arg Leu Val Ala Ser Pro Ala Phe Ala Val Asp Asp Ala Ala 510 515 520 gcg atc cac gcc gat acg ctg tcc ccc cat gtc ggc ttg ctg cgc gcg 2175 Ala Ile His Ala Asp Thr Leu Ser Pro His Val Gly Leu Leu Arg Ala 525 530 535 agg ctc gaa gcg ctc gga atc cag ggc agt ctc cct gcc gaa gag ttg 2223 Arg Leu Glu Ala Leu Gly Ile Gln Gly Ser Leu Pro Ala Glu Glu Leu 540 545 550 agg cag acc ctc atc gcc tgg gac ggc cgc atg gat gct ggc tcg cag 2271 Arg Gln Thr Leu Ile Ala Trp Asp Gly Arg Met Asp Ala Gly Ser Gln 555 560 565 570 gcg gct tcc gct tat aat gcg ttc cgc agg gcg ctg acg cgg ctg gta 2319 Ala Ala Ser Ala Tyr Asn Ala Phe Arg Arg Ala Leu Thr Arg Leu Val 575 580 585 acg gcc cgc agc ggg ctg gag caa gcg ata gcg cat ccc ttc gcg gcc 2367 Thr Ala Arg Ser Gly Leu Glu Gln Ala Ile Ala His Pro Phe Ala Ala 590 595 600 gtc ccg ccc ggc gtc tcg ccg cag ggg cag gtc tgg tgg gcc gtg ccg 2415 Val Pro Pro Gly Val Ser Pro Gln Gly Gln Val Trp Trp Ala Val Pro 605 610 615 acc ctg ctg cgc aac gac gat gcc ggg atg ctg aaa ggc tgg agc tgg 2463 Thr Leu Leu Arg Asn Asp Asp Ala Gly Met Leu Lys Gly Trp Ser Trp 620 625 630 gac gag gcc ttg tcg gag gcc ctg tcc gtc gcg acg cag aac ctg acc 2511 Asp Glu Ala Leu Ser Glu Ala Leu Ser Val Ala Thr Gln Asn Leu Thr 635 640 645 650 ggg cgc ggc tgg ggc gag gag cat cgg ccg cgt ttc acg cac ccg ctc 2559 Gly Arg Gly Trp Gly Glu Glu His Arg Pro Arg Phe Thr His Pro Leu 655 660 665 tcc gcg cag ttc ccg gcc tgg gcc gcg ctg ctg aac ccg gtt tcg cgc 2607 Ser Ala Gln Phe Pro Ala Trp Ala Ala Leu Leu Asn Pro Val Ser Arg 670 675 680 ccg atc ggc ggc gat ggc gac acc gtg ctg gcg aac ggg ctc gtc cca 2655 Pro Ile Gly Gly Asp Gly Asp Thr Val Leu Ala Asn Gly Leu Val Pro 685 690 695 tcg gcc gga cct gag gcg acc tat ggc gcc ctg tcg cgc tac gtc ttc 2703 Ser Ala Gly Pro Glu Ala Thr Tyr Gly Ala Leu Ser Arg Tyr Val Phe 700 705 710 gat gtc ggc aat tgg gac aat agc cgc tgg gtc gtc ttc cac ggc gcc 2751 Asp Val Gly Asn Trp Asp Asn Ser Arg Trp Val Val Phe His Gly Ala 715 720 725 730 tcg ggg cat ccg gcc agc ccc cac tat gcc gac cag aat gcg cca tgg 2799 Ser Gly His Pro Ala Ser Pro His Tyr Ala Asp Gln Asn Ala Pro Trp 735 740 745 agc gac tgc gcg atg gtg ccg atg ctc tat agc tgg gac agg atc gcc 2847 Ser Asp Cys Ala Met Val Pro Met Leu Tyr Ser Trp Asp Arg Ile Ala 750 755 760 gcg gag gcc gtg acc tcg cag gaa ctc gtc ccg gcc tga ggggcaaggc 2896 Ala Glu Ala Val Thr Ser Gln Glu Leu Val Pro Ala 765 770 tgcggtcagc ctgccgcagc attcttgcgg caggcgcggg tgcgtaagcc cgctgtttcg 2956 ccgccgtcga cggtcaggac ggcgccgttc acatagctcg acgcgtcgga caggagccag 3016 gccgcgagat cggcgacctc gtccggcgtg ccgagccggc ctgccggaat gcgcagctca 3076 agctgctcca gccgcagcgg gtcggccagc accttgtcca tcatccgcgt cgcgatctgc 3136 ccggg 3141 < 210 > 2 < 211 > 2847 < 212 > DNA < 213 > Brevundimonas diminuta N - 176 < 220 > < 221 > CDS < 222 > ( 483 ) . .( 2801 ) < 400 > 2 cccggggatc tcgcagacgg ctggcgcggt cctggccagc aatatgcgca aggccggctt 60 cacggtggaa cagcaggtga tggattgggg cacggtgctc gcccgccggg ccaagaagga 120 cggctggagc gttttcccgg tctacgccaa cggcatcgac atgatgtcgc cgctgacgca 180 tttctacatc ggcaacaact gcgtgaacta tgcgggctgg agctgcgacg ccgtcatcac 240 cgaaaagctc gccgcctatg ccaaggcgcc cgatccggct acccgcaaac gcatcgcggc 300 cgaaatccag gtcgaggcct acaaggacac gccctccgtg atgtggggcc agttcagccg 360 gccggcgggc taccgcctgc gcctcaagaa catcgtccag tccagcttcc cgatcttctg 420 gcagctcacg ctcgacgcgt gagcttgccc agattccgac aagcaatgag gtcccgacgc 480 ga atg act atg gcg gcc aac acc gat cgc gcg gtc ttg cag gcg gcg 527 Met Thr Met Ala Ala Asn Thr Asp Arg Ala Val Leu Gln Ala Ala 1 5 10 15 ctg ccg ccg ctt tcc ggc agc ctc ccc att ccc gga ttg agc gcg tcg 575 Leu Pro Pro Leu Ser Gly Ser Leu Pro Ile Pro Gly Leu Ser Ala Ser 20 25 30 gtc cgc gtc cgg cgc gat gcc tgg ggc atc ccg cat atc aag gcc tcg 623 Val Arg Val Arg Arg Asp Ala Trp Gly Ile Pro His Ile Lys Ala Ser 35 40 45 ggc gag gcc gat gcc tat cgg gcg ctg ggc ttc gtc cat tcg cag gac 671 Gly Glu Ala Asp Ala Tyr Arg Ala Leu Gly Phe Val His Ser Gln Asp 50 55 60 cgt ctt ttc cag atg gag ctg acg cgt cgc aag gcg ctg gga cgc gcg 719 Arg Leu Phe Gln Met Glu Leu Thr Arg Arg Lys Ala Leu Gly Arg Ala 65 70 75 gcc gaa tgg ctg ggc gcc gag gcc gcc gag gcc gat atc ctc gtg cgc 767 Ala Glu Trp Leu Gly Ala Glu Ala Ala Glu Ala Asp Ile Leu Val Arg 80 85 90 95 cgg ctc gga atg gaa aaa gtc tgc cgg cgc gac ttc gag gcc ttg ggc 815 Arg Leu Gly Met Glu Lys Val Cys Arg Arg Asp Phe Glu Ala Leu Gly 100 105 110 gtc gag gcg aag gac atg ctg cgg gct tat gtc gcc ggc gtg aac gca 863 Val Glu Ala Lys Asp Met Leu Arg Ala Tyr Val Ala Gly Val Asn Ala 115 120 125 ttc ctg gct tcc ggt gct ccc ctg cct gtc gaa tac gga ttg ctc gga 911 Phe Leu Ala Ser Gly Ala Pro Leu Pro Val Glu Tyr Gly Leu Leu Gly 130 135 140 gca gag ccg gag ccc tgg gag cct tgg cac agc atc gcg gtg atg cgc 959 Ala Glu Pro Glu Pro Trp Glu Pro Trp His Ser Ile Ala Val Met Arg 145 150 155 cgg ctg ggc ctg ctt atg ggt tcg gtg tgg ttc aag ctc tgg cgg atc 1007 Arg Leu Gly Leu Leu Met Gly Ser Val Trp Phe Lys Leu Trp Arg Ile 160 165 170 175 ctg gcg ctg ccg gtg gtc gga gcc gcc aat gcg ctg aag ctg cgc tat 1055 Leu Ala Leu Pro Val Val Gly Ala Ala Asn Ala Leu Lys Leu Arg Tyr 180 185 190 gac gat ggc ggc cgg gat ttg ctc tgc atc ccg ccg ggc gcc gaa gcc 1103 Asp Asp Gly Gly Arg Asp Leu Leu Cys Ile Pro Pro Gly Ala Glu Ala 195 200 205 gat cgg ctc gag gcg gat ctc gcg acc ctg cgg ccc gcg gtc gat gcg 1151 Asp Arg Leu Glu Ala Asp Leu Ala Thr Leu Arg Pro Ala Val Asp Ala 210 215 220 ctg ctg aag gcg atg ggc ggc gat gcc tcc gat gct gcc ggc ggc ggc 1199 Leu Leu Lys Ala Met Gly Gly Asp Ala Ser Asp Ala Ala Gly Gly Gly 225 230 235 agc aac aac tgg gcg gtc gct ccg ggc cgc acg gcg acc ggc agg ccg 1247 Ser Asn Asn Trp Ala Val Ala Pro Gly Arg Thr Ala Thr Gly Arg Pro 240 245 250 255 atc ctc gcg ggc gat ccg cat cgc gtc ttc gaa atc ccg ggc atg tat 1295 Ile Leu Ala Gly Asp Pro His Arg Val Phe Glu Ile Pro Gly Met Tyr 260 265 270 gcg cag cat cat ctg gcc tgc gac cgg ttc gac atg atc ggc ctg acc 1343 Ala Gln His His Leu Ala Cys Asp Arg Phe Asp Met Ile Gly Leu Thr 275 280 285 gtg ccg ggc gtg ccg ggc ttc ccg cac ttc gcg cat aac ggc aag gtc 1391 Val Pro Gly Val Pro Gly Phe Pro His Phe Ala His Asn Gly Lys Val 290 295 300 gcc tat tgc gtc acc cat gcc ttc atg gac atc cac gat ctc tat ctc 1439 Ala Tyr Cys Val Thr His Ala Phe Met Asp Ile His Asp Leu Tyr Leu 305 310 315 gag cag ttc gcg ggg gag ggc cgc act gcg cgg ttc ggc aac gat ttc 1487 Glu Gln Phe Ala Gly Glu Gly Arg Thr Ala Arg Phe Gly Asn Asp Phe 320 325 330 335 gag ccc gtc gcc tgg agc cgg gac cgt atc gcg gtc cgg ggt ggc gcc 1535 Glu Pro Val Ala Trp Ser Arg Asp Arg Ile Ala Val Arg Gly Gly Ala 340 345 350 gat cgc gag ttc gat atc gtc gag acg cgc cat ggc ccg gtt atc gcg 1583 Asp Arg Glu Phe Asp Ile Val Glu Thr Arg His Gly Pro Val Ile Ala 355 360 365 ggc gat ccg cgc gat ggc gca gcg ctc acg ctg cgt tcg gtc cag ttc 1631 Gly Asp Pro Arg Asp Gly Ala Ala Leu Thr Leu Arg Ser Val Gln Phe 370 375 380 gcc gag acc gat ctg tcc ttc gac tgc ctg acg cgg atg ccg ggc gca 1679 Ala Glu Thr Asp Leu Ser Phe Asp Cys Leu Thr Arg Met Pro Gly Ala 385 390 395 tcg acc gtg gcc cag ctc tac gac gcg acg cgc ggc tgg ggc ctg atc 1727 Ser Thr Val Ala Gln Leu Tyr Asp Ala Thr Arg Gly Trp Gly Leu Ile 400 405 410 415 gac cat aac ctc gtc gcc ggg gat gtc gcg ggc tcg atc ggc cat ctg 1775 Asp His Asn Leu Val Ala Gly Asp Val Ala Gly Ser Ile Gly His Leu 420 425 430 gtc cgc gcc cgc gtt ccg tcc cgt ccg cgc gaa aac ggc tgg ctg ccg 1823 Val Arg Ala Arg Val Pro Ser Arg Pro Arg Glu Asn Gly Trp Leu Pro 435 440 445 gtg ccg ggc tgg tcc ggc gag cat gaa tgg cgg ggc tgg att ccg cac 1871 Val Pro Gly Trp Ser Gly Glu His Glu Trp Arg Gly Trp Ile Pro His 450 455 460 gag gcg atg ccg cgc gtg atc gat ccg ccg ggc ggc atc atc gtc acg 1919 Glu Ala Met Pro Arg Val Ile Asp Pro Pro Gly Gly Ile Ile Val Thr 465 470 475 gcg aat aat cgc gtc gtg gcc gat gac cat ccc gat tat ctc tgc acc 1967 Ala Asn Asn Arg Val Val Ala Asp Asp His Pro Asp Tyr Leu Cys Thr 480 485 490 495 gat tgc cat ccg ccc tac cgc gcc gag cgc atc atg aag cgc ctg gtc 2015 Asp Cys His Pro Pro Tyr Arg Ala Glu Arg Ile Met Lys Arg Leu Val 500 505 510 gcc aat ccg gct ttc gcc gtc gac gat gcc gcc gcg atc cat gcc gat 2063 Ala Asn Pro Ala Phe Ala Val Asp Asp Ala Ala Ala Ile His Ala Asp 515 520 525 acg ctg tcg ccc cat gtc ggg ttg ctg cgc cgg agg ctc gag gcg ctt 2111 Thr Leu Ser Pro His Val Gly Leu Leu Arg Arg Arg Leu Glu Ala Leu 530 535 540 gga gcc cgc gac gac tcc gcg gcc gaa ggg ctg agg cag atg ctc gtc 2159 Gly Ala Arg Asp Asp Ser Ala Ala Glu Gly Leu Arg Gln Met Leu Val 545 550 555 gcc tgg gac ggc cgc atg gat gcg gct tcg gag gtc gcg tct gcc tac 2207 Ala Trp Asp Gly Arg Met Asp Ala Ala Ser Glu Val Ala Ser Ala Tyr 560 565 570 575 aat gcg ttc cgc agg gcg ctg acg cgg ctg gtg acg gac cgc agc ggg 2255 Asn Ala Phe Arg Arg Ala Leu Thr Arg Leu Val Thr Asp Arg Ser Gly 580 585 590 ctg gag cag gcg ata tcg cat ccc ttc gcg gct gtc gcg ccg ggc gtc 2303 Leu Glu Gln Ala Ile Ser His Pro Phe Ala Ala Val Ala Pro Gly Val 595 600 605 tca ccg caa ggc cag gtc tgg tgg gcc gtg ccg acc ctg ctg cgc gac 2351 Ser Pro Gln Gly Gln Val Trp Trp Ala Val Pro Thr Leu Leu Arg Asp 610 615 620 gac gat gcc gga atg ctg aag ggc tgg agc tgg gac cag gcc ttg tct 2399 Asp Asp Ala Gly Met Leu Lys Gly Trp Ser Trp Asp Gln Ala Leu Ser 625 630 635 gag gcc ctc tcg gtc gcg tcg cag aac ctg acc ggg cga agc tgg ggc 2447 Glu Ala Leu Ser Val Ala Ser Gln Asn Leu Thr Gly Arg Ser Trp Gly 640 645 650 655 gaa gag cat cgg ccg cgc ttc acg cat ccg ctt gcc acg caa ttc ccg 2495 Glu Glu His Arg Pro Arg Phe Thr His Pro Leu Ala Thr Gln Phe Pro 660 665 670 gcc tgg gcg ggg ctg ctg aat ccg gct tcc cgt ccg atc ggt ggc gat 2543 Ala Trp Ala Gly Leu Leu Asn Pro Ala Ser Arg Pro Ile Gly Gly Asp 675 680 685 ggc gat acc gtg ctg gcg aac ggg ctc gtc ccg tca gcc ggg ccg cag 2591 Gly Asp Thr Val Leu Ala Asn Gly Leu Val Pro Ser Ala Gly Pro Gln 690 695 700 gcg acc tat ggt gcc ctg tcg cgc tac gtc ttc gat gtc ggc aat tgg 2639 Ala Thr Tyr Gly Ala Leu Ser Arg Tyr Val Phe Asp Val Gly Asn Trp 705 710 715 gac aat agc cgc tgg gtc gtc ttc cac ggc gcc tcc ggg cat ccg gcc 2687 Asp Asn Ser Arg Trp Val Val Phe His Gly Ala Ser Gly His Pro Ala 720 725 730 735 agc gcc cat tat gcc gat cag aat gcg ccc tgg agc gac tgt gcg atg 2735 Ser Ala His Tyr Ala Asp Gln Asn Ala Pro Trp Ser Asp Cys Ala Met 740 745 750 gtg ccg atg ctc tat agc tgg gac agg atc gcg gca gag gcc gtg acg 2783 Val Pro Met Leu Tyr Ser Trp Asp Arg Ile Ala Ala Glu Ala Val Thr 755 760 765 tcg cag gaa ctc gtc ccg gcctgagggc cgggcctgtt gtcagcctgc 2831 Ser Gln Glu Leu Val Pro 770 cgcagctctc ttcggc 2847 < 210 > 3 < 211 > 2325 < 212 > DNA < 213 > Brevundimonas diminuta V 22 < 220 > < 221 > CDS < 222 > ( 1 ) . .( 2322 ) < 400 > 3 atg act atg gct gcc aac acc gat cgc gcc gtc ttg cag gcg gcg ctg 48 Met Thr Met Ala Ala Asn Thr Asp Arg Ala Val Leu Gln Ala Ala Leu 1 5 10 15 ccg ccg ctt tcc ggc agc ctc ccc att ccc gga ttg agc gcg tcg gtc 96 Pro Pro Leu Ser Gly Ser Leu Pro Ile Pro Gly Leu Ser Ala Ser Val 20 25 30 cct atc cag cgc gat gcc tgg ggc atc ccg cat atc aag gcc tcc ggc 144 Pro Ile Gln Arg Asp Ala Trp Gly Ile Pro His Ile Lys Ala Ser Gly 35 40 45 gag gcc gat gcc tat cgc gcg ctg ggc ttc gtc cat gcg cag gac cgc 192 Glu Ala Asp Ala Tyr Arg Ala Leu Gly Phe Val His Ala Gln Asp Arg 50 55 60 ctt ttc cag atg gag ctg acg cgt cgc aag gcg ctg gga cgc gcg gcc 240 Leu Phe Gln Met Glu Leu Thr Arg Arg Lys Ala Leu Gly Arg Ala Ala 65 70 75 80 gaa tgg ctg ggt gcc gag gcc gcc gag gcc gat atc ctc gtg cgc cgg 288 Glu Trp Leu Gly Ala Glu Ala Ala Glu Ala Asp Ile Leu Val Arg Arg 85 90 95 ctc ggt atg gaa aaa gtc tgc cga cgc gat ttc gag gcc ctg ggc gcc 336 Leu Gly Met Glu Lys Val Cys Arg Arg Asp Phe Glu Ala Leu Gly Ala 100 105 110 gag gcg aag gac atg ctc cgg gcc tac gtc gcc ggc gtg aac gca ttc 384 Glu Ala Lys Asp Met Leu Arg Ala Tyr Val Ala Gly Val Asn Ala Phe 115 120 125 ctg gct tcc ggt gtt ccc ctg cct gtc gaa tac gga ttg ctc gga gca 432 Leu Ala Ser Gly Val Pro Leu Pro Val Glu Tyr Gly Leu Leu Gly Ala 130 135 140 gag ccg gag ccc tgg gag cct tgg cac agc atc gcg gtg atg cgc cgg 480 Glu Pro Glu Pro Trp Glu Pro Trp His Ser Ile Ala Val Met Arg Arg 145 150 155 160 ctg ggc ctg ctg atg ggt tcg gtc tgg ttc aag ctc tgg cgg atg ctg 528 Leu Gly Leu Leu Met Gly Ser Val Trp Phe Lys Leu Trp Arg Met Leu 165 170 175 gcg ctg ccg gtg gtc gga gcc gcg aat gcg ctg aag ctg cgc tat gac 576 Ala Leu Pro Val Val Gly Ala Ala Asn Ala Leu Lys Leu Arg Tyr Asp 180 185 190 gat ggc ggc cgc gat ttg ctc tgc atc ccg ccg cgc gcc gaa gcg gat 624 Asp Gly Gly Arg Asp Leu Leu Cys Ile Pro Pro Arg Ala Glu Ala Asp 195 200 205 cgg ctc gag gcg gat ctc gcg acc ctg cgg ccc gcg gtc gat gcg ctg 672 Arg Leu Glu Ala Asp Leu Ala Thr Leu Arg Pro Ala Val Asp Ala Leu 210 215 220 ctg aag gcg atg ggc ggg gat gcc tca gat gcc gcc ggt ggc ggc agc 720 Leu Lys Ala Met Gly Gly Asp Ala Ser Asp Ala Ala Gly Gly Gly Ser 225 230 235 240 aac aac tgg gcg gtc gcg ccg ggc cgt acg gcg acc ggc cgg ccg atc 768 Asn Asn Trp Ala Val Ala Pro Gly Arg Thr Ala Thr Gly Arg Pro Ile 245 250 255 ctc gcg ggc gat ccg cat cgc gtc ttc cag atc ccc ggc atg tat gcc 816 Leu Ala Gly Asp Pro His Arg Val Phe Gln Ile Pro Gly Met Tyr Ala 260 265 270 cag cat cat ctg gcc tgc gat cgc ttc gac atg atc ggc ctg acc gtg 864 Gln His His Leu Ala Cys Asp Arg Phe Asp Met Ile Gly Leu Thr Val 275 280 285 ccg ggc gtg ccg ggt ttt ccg cat ttc gcg cat aac ggc aag gtc gcc 912 Pro Gly Val Pro Gly Phe Pro His Phe Ala His Asn Gly Lys Val Ala 290 295 300 tac tgc gtc acc cat gcc ttc atg gac att cac gat ctc tac ctt gag 960 Tyr Cys Val Thr His Ala Phe Met Asp Ile His Asp Leu Tyr Leu Glu 305 310 315 320 cag ttc gcg gag gag ggc cgc agg gcg cgg ttc ggc aac gat ttc gag 1008 Gln Phe Ala Glu Glu Gly Arg Arg Ala Arg Phe Gly Asn Asp Phe Glu 325 330 335 ccc gcc gcc tgg agc cgg gac cgt atc gcg gtc cgg ggt ggt gcc gac 1056 Pro Ala Ala Trp Ser Arg Asp Arg Ile Ala Val Arg Gly Gly Ala Asp 340 345 350 cgc gaa ttc gat atc atc gag acg cgc cat ggt ccc gtc ata gca ggc 1104 Arg Glu Phe Asp Ile Ile Glu Thr Arg His Gly Pro Val Ile Ala Gly 355 360 365 gat ccg cgc gat ggc gca gcg ctc acg ctg cgc tcg gtc cag ttc gcc 1152 Asp Pro Arg Asp Gly Ala Ala Leu Thr Leu Arg Ser Val Gln Phe Ala 370 375 380 gag acc gat ctg tcc ttc gat tgc ctg acg cgg atg ccg ggc gca tcg 1200 Glu Thr Asp Leu Ser Phe Asp Cys Leu Thr Arg Met Pro Gly Ala Ser 385 390 395 400 acc gtg gcg cag ctc tac gac gcg acg cgc ggc tgg ggc ctg atc gac 1248 Thr Val Ala Gln Leu Tyr Asp Ala Thr Arg Gly Trp Gly Leu Ile Asp 405 410 415 cat aat ctc gtc gcc ggg gat gtc ggg ggc tcg atc ggc cat ctg gtc 1296 His Asn Leu Val Ala Gly Asp Val Gly Gly Ser Ile Gly His Leu Val 420 425 430 cgc gcc cgt gtc ccg tcc cgc tcg cgc gaa aac ggc tgg ctg ccg gtg 1344 Arg Ala Arg Val Pro Ser Arg Ser Arg Glu Asn Gly Trp Leu Pro Val 435 440 445 ccg ggc tgg tcc ggc gag cat gaa tgg cgg ggt tgg att ccg cac gag 1392 Pro Gly Trp Ser Gly Glu His Glu Trp Arg Gly Trp Ile Pro His Glu 450 455 460 gcg atg ccg cgc gtg atc gat ccg ccg ggc ggc atc atc gtc acg gcg 1440 Ala Met Pro Arg Val Ile Asp Pro Pro Gly Gly Ile Ile Val Thr Ala 465 470 475 480 aat aat cgc gtc gtg gcc gat gac cat ccc gat tat ctc tgc acc gat 1488 Asn Asn Arg Val Val Ala Asp Asp His Pro Asp Tyr Leu Cys Thr Asp 485 490 495 tgc cat ccg ccc tac cgc gcc gag ccc atc atg aag cgc ctg gtc gcc 1536 Cys His Pro Pro Tyr Arg Ala Glu Pro Ile Met Lys Arg Leu Val Ala 500 505 510 aat ccg gct ttc gcc gtc gac gat gcc gcc gcg atc cat gcc gat acg 1584 Asn Pro Ala Phe Ala Val Asp Asp Ala Ala Ala Ile His Ala Asp Thr 515 520 525 ctg tcg ccc cat gtc ggg ttg ctg cgc cgg agg ctc gag gcg ctt gga 1632 Leu Ser Pro His Val Gly Leu Leu Arg Arg Arg Leu Glu Ala Leu Gly 530 535 540 gcc cgc gac gac tcc gcg gcc gaa ggg ctg agg cag atg ctc gtc gcc 1680 Ala Arg Asp Asp Ser Ala Ala Glu Gly Leu Arg Gln Met Leu Val Ala 545 550 555 560 tgg gac ggc cgc atg gat gcg gct tcg gag gtc gcg tct gcc tac aat 1728 Trp Asp Gly Arg Met Asp Ala Ala Ser Glu Val Ala Ser Ala Tyr Asn 565 570 575 gcg ttc cgc agg gcg ctg acg cgg ctg gtg acg gac cgc agc ggg ctg 1776 Ala Phe Arg Arg Ala Leu Thr Arg Leu Val Thr Asp Arg Ser Gly Leu 580 585 590 gag cag gcg ata tcg cat ccc ttc gcg gct gtc gcg ccg ggc gtc tca 1824 Glu Gln Ala Ile Ser His Pro Phe Ala Ala Val Ala Pro Gly Val Ser 595 600 605 ccg caa ggc cag gtc tgg tgg gcc gtg ccg acc ctg ctg cgc gac gac 1872 Pro Gln Gly Gln Val Trp Trp Ala Val Pro Thr Leu Leu Arg Asp Asp 610 615 620 gat gcc gga atg ctg aag ggc tgg agc tgg gac cag gcc ttg tct gag 1920 Asp Ala Gly Met Leu Lys Gly Trp Ser Trp Asp Gln Ala Leu Ser Glu 625 630 635 640 gcc ctc tcg gtc gcg tcg cag aac ctg agc cgg cga agc tgg ggc gaa 1968 Ala Leu Ser Val Ala Ser Gln Asn Leu Ser Arg Arg Ser Trp Gly Glu 645 650 655 gag cat cgg ccg cgc ttc acg cat ccg ctt gcc acg caa ttc ccg gcc 2016 Glu His Arg Pro Arg Phe Thr His Pro Leu Ala Thr Gln Phe Pro Ala 660 665 670 tgg gcg ggg ctg ctg aat ccg gct tcc cgt ccg atc ggc ggc gat ggc 2064 Trp Ala Gly Leu Leu Asn Pro Ala Ser Arg Pro Ile Gly Gly Asp Gly 675 680 685 gac acc gtg ctg gcg aac ggg ctc gtc ccg tca gcc ggg ccg cag gcg 2112 Asp Thr Val Leu Ala Asn Gly Leu Val Pro Ser Ala Gly Pro Gln Ala 690 695 700 acc tat ggc gcc ctg tcg cgc tac gtc ttt gat gtc ggc aat tgg gac 2160 Thr Tyr Gly Ala Leu Ser Arg Tyr Val Phe Asp Val Gly Asn Trp Asp 705 710 715 720 aat agc cgc tgg gtc gtc ttc cac ggc gcc tcc ggg cat ccg gcc agc 2208 Asn Ser Arg Trp Val Val Phe His Gly Ala Ser Gly His Pro Ala Ser 725 730 735 gcc cat tat gcc gat cag aat gcg ccc tgg agc gac tgt gcg atg gtg 2256 Ala His Tyr Ala Asp Gln Asn Ala Pro Trp Ser Asp Cys Ala Met Val 740 745 750 ccg atg ctc tat agc tgg gac agg atc gcg gca gag gcc gtg acg tcg 2304 Pro Met Leu Tyr Ser Trp Asp Arg Ile Ala Ala Glu Ala Val Thr Ser 755 760 765 cag gaa ctc gtc ccg gcc tga 2325 Gln Glu Leu Val Pro Ala 770