6 Kozak - SP - GFP G A A T T C A C C A T G A A T C T T C G T T G T A T C C T T C C G T T T C T C C T C G C A A G C T T C T C G G C T G G G G C TAGCATGGTGAGCAAGGGCGAGGAG EcoRI - NheI Long primer for SP amplification ; PCR