< 110 > PEPGENE INC . < 120 > METHOD FOR PRODUCTION OF GLUCAGON - LIKE PEPTIDE - 1 OR ANALOGUES WITH GROES PUSION < 130 > YP - 21 - 104 < 150 > KR 10 - 2019 - 0087637 < 151 > 2019 - 07 - 19 < 160 > 89 < 170 > KoPatentIn 3 . 0 < 210 > 1 < 211 > 43 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 43 < 400 > 1 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val 35 40 < 210 > 2 < 211 > 57 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 57 < 400 > 2 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu 50 55 < 210 > 3 < 211 > 68 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 68 < 400 > 3 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn 65 < 210 > 4 < 211 > 79 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 79 < 400 > 4 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp 65 70 75 < 210 > 5 < 211 > 88 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 88 < 400 > 5 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu 85 < 210 > 6 < 211 > 97 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 97 < 400 > 6 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu 85 90 95 Ala < 210 > 7 < 211 > 98 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for SUMO < 400 > 7 Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro 1 5 10 15 Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 20 25 30 Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 35 40 45 Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 50 55 60 Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp 65 70 75 80 Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile 85 90 95 Gly Gly < 210 > 8 < 211 > 6 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for H 6 < 400 > 8 His His His His His His 1 5 < 210 > 9 < 211 > 6 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Tobacco etch virus ( TEV ) protease recognition site < 400 > 9 Glu Asn Leu Tyr Phe Gln 1 5 < 210 > 10 < 211 > 13 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for H 6 TEV < 400 > 10 Met His His His His His His Glu Asn Leu Tyr Phe Gln 1 5 10 < 210 > 11 < 211 > 57 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 43 - H 6 TEV < 400 > 11 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Pro Trp His His His 35 40 45 His His His Glu Asn Leu Tyr Phe Gln 50 55 < 210 > 12 < 211 > 71 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 57 - H 6 TEV < 400 > 12 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Pro Trp His His His His His 50 55 60 His Glu Asn Leu Tyr Phe Gln 65 70 < 210 > 13 < 211 > 82 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 68 - H 6 TEV < 400 > 13 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Pro Trp His His His His His His Glu Asn Leu Tyr 65 70 75 80 Phe Gln < 210 > 14 < 211 > 93 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 79 - H 6 TEV < 400 > 14 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Pro 65 70 75 80 Trp His His His His His His Glu Asn Leu Tyr Phe Gln 85 90 < 210 > 15 < 211 > 102 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 88 - H 6 TEV < 400 > 15 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu Pro Trp His His His His His His 85 90 95 Glu Asn Leu Tyr Phe Gln 100 < 210 > 16 < 211 > 109 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 97 - H 6 TEV < 400 > 16 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu 85 90 95 Ala His His His His His His Glu Asn Leu Tyr Phe Gln 100 105 < 210 > 17 < 211 > 31 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for Liraglutide precursor peptide ( GLP - 1 K 28 R ) < 400 > 17 His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 1 5 10 15 Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 20 25 30 < 210 > 18 < 211 > 44 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for H 6 TEV - GLP - 1 K 28 R < 400 > 18 Met His His His His His His Glu Asn Leu Tyr Phe Gln His Ala Glu 1 5 10 15 Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala Ala 20 25 30 Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 35 40 < 210 > 19 < 211 > 88 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 43 - H 6 TEV - GLP - 1 K 28 R < 400 > 19 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Pro Trp His His His 35 40 45 His His His Glu Asn Leu Tyr Phe Gln His Ala Glu Gly Thr Phe Thr 50 55 60 Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile 65 70 75 80 Ala Trp Leu Val Arg Gly Arg Gly 85 < 210 > 20 < 211 > 102 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 57 - H 6 TEV - GLP - 1 K 28 R < 400 > 20 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Pro Trp His His His His His 50 55 60 His Glu Asn Leu Tyr Phe Gln His Ala Glu Gly Thr Phe Thr Ser Asp 65 70 75 80 Val Ser Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp 85 90 95 Leu Val Arg Gly Arg Gly 100 < 210 > 21 < 211 > 113 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 68 - H 6 TEV - GLP - 1 K 28 R < 400 > 21 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Pro Trp His His His His His His Glu Asn Leu Tyr 65 70 75 80 Phe Gln His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu 85 90 95 Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg 100 105 110 Gly < 210 > 22 < 211 > 124 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 79 - H 6 TEV - GLP - 1 K 28 R < 400 > 22 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Pro 65 70 75 80 Trp His His His His His His Glu Asn Leu Tyr Phe Gln His Ala Glu 85 90 95 Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala Ala 100 105 110 Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 115 120 < 210 > 23 < 211 > 133 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 88 - H 6 TEV - GLP - 1 K 28 R < 400 > 23 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu Pro Trp His His His His His His 85 90 95 Glu Asn Leu Tyr Phe Gln His Ala Glu Gly Thr Phe Thr Ser Asp Val 100 105 110 Ser Ser Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu 115 120 125 Val Arg Gly Arg Gly 130 < 210 > 24 < 211 > 140 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 97 - H 6 TEV - GLP - 1 K 28 R < 400 > 24 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu 85 90 95 Ala His His His His His His Glu Asn Leu Tyr Phe Gln His Ala Glu 100 105 110 Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala Ala 115 120 125 Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 130 135 140 < 210 > 25 < 211 > 141 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for SUMO - H 6 TEV - GLP - 1 K 28 R < 400 > 25 Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro 1 5 10 15 Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 20 25 30 Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 35 40 45 Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 50 55 60 Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp 65 70 75 80 Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile 85 90 95 Gly Gly His His His His His His Glu Asn Leu Tyr Phe Gln His Ala 100 105 110 Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala 115 120 125 Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 130 135 140 < 210 > 26 < 211 > 34 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for hPTH 1 - 34 ( Teriparatide ) < 400 > 26 Ser Val Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His Leu Asn 1 5 10 15 Ser Met Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp Val His 20 25 30 Asn Phe < 210 > 27 < 211 > 47 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for H 6 TEV - hPTH 1 - 34 < 400 > 27 Met His His His His His His Glu Asn Leu Tyr Phe Gln Ser Val Ser 1 5 10 15 Glu Ile Gln Leu Met His Asn Leu Gly Lys His Leu Asn Ser Met Glu 20 25 30 Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp Val His Asn Phe 35 40 45 < 210 > 28 < 211 > 143 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 97 - H 6 TEV - hPTH 1 - 34 < 400 > 28 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu 85 90 95 Ala His His His His His His Glu Asn Leu Tyr Phe Gln Ser Val Ser 100 105 110 Glu Ile Gln Leu Met His Asn Leu Gly Lys His Leu Asn Ser Met Glu 115 120 125 Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp Val His Asn Phe 130 135 140 < 210 > 29 < 211 > 144 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for SUMO - H 6 TEV - hPTH 1 - 34 < 400 > 29 Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro 1 5 10 15 Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 20 25 30 Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 35 40 45 Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 50 55 60 Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp 65 70 75 80 Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile 85 90 95 Gly Gly His His His His His His Glu Asn Leu Tyr Phe Gln Ser Val 100 105 110 Ser Glu Ile Gln Leu Met His Asn Leu Gly Lys His Leu Asn Ser Met 115 120 125 Glu Arg Val Glu Trp Leu Arg Lys Lys Leu Gln Asp Val His Asn Phe 130 135 140 < 210 > 30 < 211 > 33 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for Teduglutide ( GLP - 2 A 2 G ) < 400 > 30 His Gly Asp Gly Ser Phe Ser Asp Glu Met Asn Thr Ile Leu Asp Asn 1 5 10 15 Leu Ala Ala Arg Asp Phe Ile Asn Trp Leu Ile Gln Thr Lys Ile Thr 20 25 30 Asp < 210 > 31 < 211 > 46 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for H 6 TEV - GLP - 2 A 2 G < 400 > 31 Met His His His His His His Glu Asn Leu Tyr Phe Gln His Gly Asp 1 5 10 15 Gly Ser Phe Ser Asp Glu Met Asn Thr Ile Leu Asp Asn Leu Ala Ala 20 25 30 Arg Asp Phe Ile Asn Trp Leu Ile Gln Thr Lys Ile Thr Asp 35 40 45 < 210 > 32 < 211 > 142 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 97 - H 6 TEV - GLP - 2 A 2 G < 400 > 32 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu 85 90 95 Ala His His His His His His Glu Asn Leu Tyr Phe Gln His Gly Asp 100 105 110 Gly Ser Phe Ser Asp Glu Met Asn Thr Ile Leu Asp Asn Leu Ala Ala 115 120 125 Arg Asp Phe Ile Asn Trp Leu Ile Gln Thr Lys Ile Thr Asp 130 135 140 < 210 > 33 < 211 > 143 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for SUMO - H 6 TEV - GLP - 2 A 2 G < 400 > 33 Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro 1 5 10 15 Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 20 25 30 Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 35 40 45 Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 50 55 60 Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp 65 70 75 80 Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile 85 90 95 Gly Gly His His His His His His Glu Asn Leu Tyr Phe Gln His Gly 100 105 110 Asp Gly Ser Phe Ser Asp Glu Met Asn Thr Ile Leu Asp Asn Leu Ala 115 120 125 Ala Arg Asp Phe Ile Asn Trp Leu Ile Gln Thr Lys Ile Thr Asp 130 135 140 < 210 > 34 < 211 > 60 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for Ecallantide < 400 > 34 Glu Ala Met His Ser Phe Cys Ala Phe Lys Ala Asp Asp Gly Pro Cys 1 5 10 15 Arg Ala Ala His Pro Arg Trp Phe Phe Asn Ile Phe Thr Arg Gln Cys 20 25 30 Glu Glu Phe Ile Tyr Gly Gly Cys Glu Gly Asn Gln Asn Arg Phe Glu 35 40 45 Ser Leu Glu Glu Cys Lys Lys Met Cys Thr Arg Asp 50 55 60 < 210 > 35 < 211 > 73 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for H 6 TEV - Ecallantide < 400 > 35 Met His His His His His His Glu Asn Leu Tyr Phe Gln Glu Ala Met 1 5 10 15 His Ser Phe Cys Ala Phe Lys Ala Asp Asp Gly Pro Cys Arg Ala Ala 20 25 30 His Pro Arg Trp Phe Phe Asn Ile Phe Thr Arg Gln Cys Glu Glu Phe 35 40 45 Ile Tyr Gly Gly Cys Glu Gly Asn Gln Asn Arg Phe Glu Ser Leu Glu 50 55 60 Glu Cys Lys Lys Met Cys Thr Arg Asp 65 70 < 210 > 36 < 211 > 169 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 97 - H 6 TEV - Ecallantide < 400 > 36 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu 85 90 95 Ala His His His His His His Glu Asn Leu Tyr Phe Gln Glu Ala Met 100 105 110 His Ser Phe Cys Ala Phe Lys Ala Asp Asp Gly Pro Cys Arg Ala Ala 115 120 125 His Pro Arg Trp Phe Phe Asn Ile Phe Thr Arg Gln Cys Glu Glu Phe 130 135 140 Ile Tyr Gly Gly Cys Glu Gly Asn Gln Asn Arg Phe Glu Ser Leu Glu 145 150 155 160 Glu Cys Lys Lys Met Cys Thr Arg Asp 165 < 210 > 37 < 211 > 170 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for SUMO - H 6 TEV - Ecallantide < 400 > 37 Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro 1 5 10 15 Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 20 25 30 Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 35 40 45 Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 50 55 60 Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp 65 70 75 80 Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile 85 90 95 Gly Gly His His His His His His Glu Asn Leu Tyr Phe Gln Glu Ala 100 105 110 Met His Ser Phe Cys Ala Phe Lys Ala Asp Asp Gly Pro Cys Arg Ala 115 120 125 Ala His Pro Arg Trp Phe Phe Asn Ile Phe Thr Arg Gln Cys Glu Glu 130 135 140 Phe Ile Tyr Gly Gly Cys Glu Gly Asn Gln Asn Arg Phe Glu Ser Leu 145 150 155 160 Glu Glu Cys Lys Lys Met Cys Thr Arg Asp 165 170 < 210 > 38 < 211 > 32 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for Nesiritide < 400 > 38 Ser Pro Lys Met Val Gln Gly Ser Gly Cys Phe Gly Arg Lys Met Asp 1 5 10 15 Arg Ile Ser Ser Ser Ser Gly Leu Gly Cys Lys Val Leu Arg Arg His 20 25 30 < 210 > 39 < 211 > 45 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for H 6 TEV - Nesiritide < 400 > 39 Met His His His His His His Glu Asn Leu Tyr Phe Gln Ser Pro Lys 1 5 10 15 Met Val Gln Gly Ser Gly Cys Phe Gly Arg Lys Met Asp Arg Ile Ser 20 25 30 Ser Ser Ser Gly Leu Gly Cys Lys Val Leu Arg Arg His 35 40 45 < 210 > 40 < 211 > 141 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 97 - H 6 TEV - Nesiritide < 400 > 40 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu 85 90 95 Ala His His His His His His Glu Asn Leu Tyr Phe Gln Ser Pro Lys 100 105 110 Met Val Gln Gly Ser Gly Cys Phe Gly Arg Lys Met Asp Arg Ile Ser 115 120 125 Ser Ser Ser Gly Leu Gly Cys Lys Val Leu Arg Arg His 130 135 140 < 210 > 41 < 211 > 142 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for SUMO - H 6 TEV - Nesiritide < 400 > 41 Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro 1 5 10 15 Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 20 25 30 Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 35 40 45 Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 50 55 60 Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp 65 70 75 80 Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile 85 90 95 Gly Gly His His His His His His Glu Asn Leu Tyr Phe Gln Ser Pro 100 105 110 Lys Met Val Gln Gly Ser Gly Cys Phe Gly Arg Lys Met Asp Arg Ile 115 120 125 Ser Ser Ser Ser Gly Leu Gly Cys Lys Val Leu Arg Arg His 130 135 140 < 210 > 42 < 211 > 53 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for Human Epidermal growth factor ( hEGF ) < 400 > 42 Asn Ser Asp Ser Glu Cys Pro Leu Ser His Asp Gly Tyr Cys Leu His 1 5 10 15 Asp Gly Val Cys Met Tyr Ile Glu Ala Leu Asp Lys Tyr Ala Cys Asn 20 25 30 Cys Val Val Gly Tyr Ile Gly Glu Arg Cys Gln Tyr Arg Asp Leu Lys 35 40 45 Trp Trp Glu Leu Arg 50 < 210 > 43 < 211 > 66 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for H 6 TEV - hEGF < 400 > 43 Met His His His His His His Glu Asn Leu Tyr Phe Gln Asn Ser Asp 1 5 10 15 Ser Glu Cys Pro Leu Ser His Asp Gly Tyr Cys Leu His Asp Gly Val 20 25 30 Cys Met Tyr Ile Glu Ala Leu Asp Lys Tyr Ala Cys Asn Cys Val Val 35 40 45 Gly Tyr Ile Gly Glu Arg Cys Gln Tyr Arg Asp Leu Lys Trp Trp Glu 50 55 60 Leu Arg 65 < 210 > 44 < 211 > 162 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 97 - H 6 TEV - hEGF < 400 > 44 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu 85 90 95 Ala His His His His His His Glu Asn Leu Tyr Phe Gln Asn Ser Asp 100 105 110 Ser Glu Cys Pro Leu Ser His Asp Gly Tyr Cys Leu His Asp Gly Val 115 120 125 Cys Met Tyr Ile Glu Ala Leu Asp Lys Tyr Ala Cys Asn Cys Val Val 130 135 140 Gly Tyr Ile Gly Glu Arg Cys Gln Tyr Arg Asp Leu Lys Trp Trp Glu 145 150 155 160 Leu Arg < 210 > 45 < 211 > 163 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for SUMO - H 6 TEV - hEGF < 400 > 45 Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro 1 5 10 15 Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 20 25 30 Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 35 40 45 Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 50 55 60 Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp 65 70 75 80 Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile 85 90 95 Gly Gly His His His His His His Glu Asn Leu Tyr Phe Gln Asn Ser 100 105 110 Asp Ser Glu Cys Pro Leu Ser His Asp Gly Tyr Cys Leu His Asp Gly 115 120 125 Val Cys Met Tyr Ile Glu Ala Leu Asp Lys Tyr Ala Cys Asn Cys Val 130 135 140 Val Gly Tyr Ile Gly Glu Arg Cys Gln Tyr Arg Asp Leu Lys Trp Trp 145 150 155 160 Glu Leu Arg < 210 > 46 < 211 > 165 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for Interferon beta 1 b ( INF - beta 1 b ) < 400 > 46 Ser Tyr Asn Leu Leu Gly Phe Leu Gln Arg Ser Ser Asn Phe Gln Ser 1 5 10 15 Gln Lys Leu Leu Trp Gln Leu Asn Gly Arg Leu Glu Tyr Cys Leu Lys 20 25 30 Asp Arg Met Asn Phe Asp Ile Pro Glu Glu Ile Lys Gln Leu Gln Gln 35 40 45 Phe Gln Lys Glu Asp Ala Ala Leu Thr Ile Tyr Glu Met Leu Gln Asn 50 55 60 Ile Phe Ala Ile Phe Arg Gln Asp Ser Ser Ser Thr Gly Trp Asn Glu 65 70 75 80 Thr Ile Val Glu Asn Leu Leu Ala Asn Val Tyr His Gln Ile Asn His 85 90 95 Leu Lys Thr Val Leu Glu Glu Lys Leu Glu Lys Glu Asp Phe Thr Arg 100 105 110 Gly Lys Leu Met Ser Ser Leu His Leu Lys Arg Tyr Tyr Gly Arg Ile 115 120 125 Leu His Tyr Leu Lys Ala Lys Glu Tyr Ser His Cys Ala Trp Thr Ile 130 135 140 Val Arg Val Glu Ile Leu Arg Asn Phe Tyr Phe Ile Asn Arg Leu Thr 145 150 155 160 Gly Tyr Leu Arg Asn 165 < 210 > 47 < 211 > 178 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for H 6 TEV - INF - beta 1 b < 400 > 47 Met His His His His His His Glu Asn Leu Tyr Phe Gln Ser Tyr Asn 1 5 10 15 Leu Leu Gly Phe Leu Gln Arg Ser Ser Asn Phe Gln Ser Gln Lys Leu 20 25 30 Leu Trp Gln Leu Asn Gly Arg Leu Glu Tyr Cys Leu Lys Asp Arg Met 35 40 45 Asn Phe Asp Ile Pro Glu Glu Ile Lys Gln Leu Gln Gln Phe Gln Lys 50 55 60 Glu Asp Ala Ala Leu Thr Ile Tyr Glu Met Leu Gln Asn Ile Phe Ala 65 70 75 80 Ile Phe Arg Gln Asp Ser Ser Ser Thr Gly Trp Asn Glu Thr Ile Val 85 90 95 Glu Asn Leu Leu Ala Asn Val Tyr His Gln Ile Asn His Leu Lys Thr 100 105 110 Val Leu Glu Glu Lys Leu Glu Lys Glu Asp Phe Thr Arg Gly Lys Leu 115 120 125 Met Ser Ser Leu His Leu Lys Arg Tyr Tyr Gly Arg Ile Leu His Tyr 130 135 140 Leu Lys Ala Lys Glu Tyr Ser His Cys Ala Trp Thr Ile Val Arg Val 145 150 155 160 Glu Ile Leu Arg Asn Phe Tyr Phe Ile Asn Arg Leu Thr Gly Tyr Leu 165 170 175 Arg Asn < 210 > 48 < 211 > 274 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 97 - H 6 TEV - INF - beta 1 b < 400 > 48 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu 85 90 95 Ala His His His His His His Glu Asn Leu Tyr Phe Gln Ser Tyr Asn 100 105 110 Leu Leu Gly Phe Leu Gln Arg Ser Ser Asn Phe Gln Ser Gln Lys Leu 115 120 125 Leu Trp Gln Leu Asn Gly Arg Leu Glu Tyr Cys Leu Lys Asp Arg Met 130 135 140 Asn Phe Asp Ile Pro Glu Glu Ile Lys Gln Leu Gln Gln Phe Gln Lys 145 150 155 160 Glu Asp Ala Ala Leu Thr Ile Tyr Glu Met Leu Gln Asn Ile Phe Ala 165 170 175 Ile Phe Arg Gln Asp Ser Ser Ser Thr Gly Trp Asn Glu Thr Ile Val 180 185 190 Glu Asn Leu Leu Ala Asn Val Tyr His Gln Ile Asn His Leu Lys Thr 195 200 205 Val Leu Glu Glu Lys Leu Glu Lys Glu Asp Phe Thr Arg Gly Lys Leu 210 215 220 Met Ser Ser Leu His Leu Lys Arg Tyr Tyr Gly Arg Ile Leu His Tyr 225 230 235 240 Leu Lys Ala Lys Glu Tyr Ser His Cys Ala Trp Thr Ile Val Arg Val 245 250 255 Glu Ile Leu Arg Asn Phe Tyr Phe Ile Asn Arg Leu Thr Gly Tyr Leu 260 265 270 Arg Asn < 210 > 49 < 211 > 275 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for SUMO - H 6 TEV - INF - beta 1 b < 400 > 49 Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro 1 5 10 15 Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 20 25 30 Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 35 40 45 Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 50 55 60 Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp 65 70 75 80 Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile 85 90 95 Gly Gly His His His His His His Glu Asn Leu Tyr Phe Gln Ser Tyr 100 105 110 Asn Leu Leu Gly Phe Leu Gln Arg Ser Ser Asn Phe Gln Ser Gln Lys 115 120 125 Leu Leu Trp Gln Leu Asn Gly Arg Leu Glu Tyr Cys Leu Lys Asp Arg 130 135 140 Met Asn Phe Asp Ile Pro Glu Glu Ile Lys Gln Leu Gln Gln Phe Gln 145 150 155 160 Lys Glu Asp Ala Ala Leu Thr Ile Tyr Glu Met Leu Gln Asn Ile Phe 165 170 175 Ala Ile Phe Arg Gln Asp Ser Ser Ser Thr Gly Trp Asn Glu Thr Ile 180 185 190 Val Glu Asn Leu Leu Ala Asn Val Tyr His Gln Ile Asn His Leu Lys 195 200 205 Thr Val Leu Glu Glu Lys Leu Glu Lys Glu Asp Phe Thr Arg Gly Lys 210 215 220 Leu Met Ser Ser Leu His Leu Lys Arg Tyr Tyr Gly Arg Ile Leu His 225 230 235 240 Tyr Leu Lys Ala Lys Glu Tyr Ser His Cys Ala Trp Thr Ile Val Arg 245 250 255 Val Glu Ile Leu Arg Asn Phe Tyr Phe Ile Asn Arg Leu Thr Gly Tyr 260 265 270 Leu Arg Asn 275 < 210 > 50 < 211 > 191 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for Human growth hormone ( hGH ) < 400 > 50 Phe Pro Thr Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg 1 5 10 15 Ala His Arg Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu 20 25 30 Glu Ala Tyr Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro 35 40 45 Gln Thr Ser Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg 50 55 60 Glu Glu Thr Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu 65 70 75 80 Leu Leu Ile Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val 85 90 95 Phe Ala Asn Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Tyr Asp 100 105 110 Leu Leu Lys Asp Leu Glu Glu Gly Ile Gln Thr Leu Met Gly Arg Leu 115 120 125 Glu Asp Gly Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Thr Tyr Ser 130 135 140 Lys Phe Asp Thr Asn Ser His Asn Asp Asp Ala Leu Leu Lys Asn Tyr 145 150 155 160 Gly Leu Leu Tyr Cys Phe Arg Lys Asp Met Asp Lys Val Glu Thr Phe 165 170 175 Leu Arg Ile Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe 180 185 190 < 210 > 51 < 211 > 204 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for H 6 TEV - hGH < 400 > 51 Met His His His His His His Glu Asn Leu Tyr Phe Gln Phe Pro Thr 1 5 10 15 Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg Ala His Arg 20 25 30 Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu Glu Ala Tyr 35 40 45 Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro Gln Thr Ser 50 55 60 Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg Glu Glu Thr 65 70 75 80 Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu Leu Leu Ile 85 90 95 Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val Phe Ala Asn 100 105 110 Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Tyr Asp Leu Leu Lys 115 120 125 Asp Leu Glu Glu Gly Ile Gln Thr Leu Met Gly Arg Leu Glu Asp Gly 130 135 140 Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Thr Tyr Ser Lys Phe Asp 145 150 155 160 Thr Asn Ser His Asn Asp Asp Ala Leu Leu Lys Asn Tyr Gly Leu Leu 165 170 175 Tyr Cys Phe Arg Lys Asp Met Asp Lys Val Glu Thr Phe Leu Arg Ile 180 185 190 Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe 195 200 < 210 > 52 < 211 > 300 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 97 - H 6 TEV - hGH < 400 > 52 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu 85 90 95 Ala His His His His His His Glu Asn Leu Tyr Phe Gln Phe Pro Thr 100 105 110 Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg Ala His Arg 115 120 125 Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu Glu Ala Tyr 130 135 140 Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro Gln Thr Ser 145 150 155 160 Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg Glu Glu Thr 165 170 175 Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu Leu Leu Ile 180 185 190 Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val Phe Ala Asn 195 200 205 Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Tyr Asp Leu Leu Lys 210 215 220 Asp Leu Glu Glu Gly Ile Gln Thr Leu Met Gly Arg Leu Glu Asp Gly 225 230 235 240 Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Thr Tyr Ser Lys Phe Asp 245 250 255 Thr Asn Ser His Asn Asp Asp Ala Leu Leu Lys Asn Tyr Gly Leu Leu 260 265 270 Tyr Cys Phe Arg Lys Asp Met Asp Lys Val Glu Thr Phe Leu Arg Ile 275 280 285 Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe 290 295 300 < 210 > 53 < 211 > 301 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for SUMO - H 6 TEV - hGH < 400 > 53 Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro 1 5 10 15 Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 20 25 30 Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 35 40 45 Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 50 55 60 Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp 65 70 75 80 Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile 85 90 95 Gly Gly His His His His His His Glu Asn Leu Tyr Phe Gln Phe Pro 100 105 110 Thr Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg Ala His 115 120 125 Arg Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu Glu Ala 130 135 140 Tyr Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro Gln Thr 145 150 155 160 Ser Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg Glu Glu 165 170 175 Thr Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu Leu Leu 180 185 190 Ile Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val Phe Ala 195 200 205 Asn Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Tyr Asp Leu Leu 210 215 220 Lys Asp Leu Glu Glu Gly Ile Gln Thr Leu Met Gly Arg Leu Glu Asp 225 230 235 240 Gly Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Thr Tyr Ser Lys Phe 245 250 255 Asp Thr Asn Ser His Asn Asp Asp Ala Leu Leu Lys Asn Tyr Gly Leu 260 265 270 Leu Tyr Cys Phe Arg Lys Asp Met Asp Lys Val Glu Thr Phe Leu Arg 275 280 285 Ile Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe 290 295 300 < 210 > 54 < 211 > 86 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for Insulin glargine precursor ( INSG ) < 400 > 54 Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 1 5 10 15 Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg 20 25 30 Glu Ala Glu Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly Gly Pro 35 40 45 Gly Ala Gly Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu Gln Lys 50 55 60 Arg Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 65 70 75 80 Leu Glu Asn Tyr Cys Gly 85 < 210 > 55 < 211 > 99 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for H 6 TEV - INSG < 400 > 55 Met His His His His His His Glu Asn Leu Tyr Phe Gln Phe Val Asn 1 5 10 15 Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys 20 25 30 Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg Glu Ala Glu 35 40 45 Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly Gly Pro Gly Ala Gly 50 55 60 Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu Gln Lys Arg Gly Ile 65 70 75 80 Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn 85 90 95 Tyr Cys Gly < 210 > 56 < 211 > 195 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for PG 97 - H 6 TEV - INSG < 400 > 56 Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 1 5 10 15 Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 20 25 30 Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 35 40 45 Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 50 55 60 Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 65 70 75 80 Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu 85 90 95 Ala His His His His His His Glu Asn Leu Tyr Phe Gln Phe Val Asn 100 105 110 Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys 115 120 125 Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg Glu Ala Glu 130 135 140 Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly Gly Pro Gly Ala Gly 145 150 155 160 Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu Gln Lys Arg Gly Ile 165 170 175 Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn 180 185 190 Tyr Cys Gly 195 < 210 > 57 < 211 > 196 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > amino acid sequence for SUMO - H 6 TEV - INSG < 400 > 57 Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro 1 5 10 15 Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 20 25 30 Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 35 40 45 Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 50 55 60 Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp 65 70 75 80 Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile 85 90 95 Gly Gly His His His His His His Glu Asn Leu Tyr Phe Gln Phe Val 100 105 110 Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val 115 120 125 Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg Glu Ala 130 135 140 Glu Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly Gly Pro Gly Ala 145 150 155 160 Gly Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu Gln Lys Arg Gly 165 170 175 Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu 180 185 190 Asn Tyr Cys Gly 195 < 210 > 58 < 211 > 135 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for H 6 TEV - GLP - 1 K 28 R < 400 > 58 atgcatcatc atcaccacca cgaaaacctg tatttccagc atgcggaagg cacctttacc 60 agcgatgtga gcagctatct ggaaggccag gcggcgaaag aatttattgc gtggctggtg 120 cgtggccgcg gctaa 135 < 210 > 59 < 211 > 267 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 43 - H 6 TEV - GLP - 1 K 28 R < 400 > 59 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa 60 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg 120 ctggctgtcc catggcatca tcatcaccac cacgaaaacc tgtatttcca gcatgcggaa 180 ggcaccttta ccagcgatgt gagcagctat ctggaaggcc aggcggcgaa agaatttatt 240 gcgtggctgg tgcgtggccg cggctaa 267 < 210 > 60 < 211 > 309 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 57 - H 6 TEV - GLP - 1 K 28 R < 400 > 60 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa 60 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg 120 ctggctgtcg gcaatggccg tatccttgaa aatggcgaag tgaagccgct gccatggcat 180 catcatcacc accacgaaaa cctgtatttc cagcatgcgg aaggcacctt taccagcgat 240 gtgagcagct atctggaagg ccaggcggcg aaagaattta ttgcgtggct ggtgcgtggc 300 cgcggctaa 309 < 210 > 61 < 211 > 342 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 68 - H 6 TEV - GLP - 1 K 28 R < 400 > 61 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa 60 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg 120 ctggctgtcg gcaatggccg tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa 180 gttggcgaca tcgttatttt caacccatgg catcatcatc accaccacga aaacctgtat 240 ttccagcatg cggaaggcac ctttaccagc gatgtgagca gctatctgga aggccaggcg 300 gcgaaagaat ttattgcgtg gctggtgcgt ggccgcggct aa 342 < 210 > 62 < 211 > 375 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 79 - H 6 TEV - GLP - 1 K 28 R < 400 > 62 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa 60 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg 120 ctggctgtcg gcaatggccg tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa 180 gttggcgaca tcgttatttt caacgatggc tacggtgtga aatctgagaa gatcgaccca 240 tggcatcatc atcaccacca cgaaaacctg tatttccagc atgcggaagg cacctttacc 300 agcgatgtga gcagctatct ggaaggccag gcggcgaaag aatttattgc gtggctggtg 360 cgtggccgcg gctaa 375 < 210 > 63 < 211 > 402 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 88 - H 6 TEV - GLP - 1 K 28 R < 400 > 63 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa 60 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg 120 ctggctgtcg gcaatggccg tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa 180 gttggcgaca tcgttatttt caacgatggc tacggtgtga aatctgagaa gatcgacaat 240 gaagaagtgt tgatcatgtc cgaaccatgg catcatcatc accaccacga aaacctgtat 300 ttccagcatg cggaaggcac ctttaccagc gatgtgagca gctatctgga aggccaggcg 360 gcgaaagaat ttattgcgtg gctggtgcgt ggccgcggct aa 402 < 210 > 64 < 211 > 423 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 97 - H 6 TEV - GLP - 1 K 28 R < 400 > 64 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa 60 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg 120 ctggctgtcg gcaatggccg tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa 180 gttggcgaca tcgttatttt caacgatggc tacggtgtga aatctgagaa gatcgacaat 240 gaagaagtgt tgatcatgtc cgaaagcgac attctggcaa ttgttgaagc gcatcatcat 300 caccaccacg aaaacctgta tttccagcat gcggaaggca cctttaccag cgatgtgagc 360 agctatctgg aaggccaggc ggcgaaagaa tttattgcgt ggctggtgcg tggccgcggc 420 taa 423 < 210 > 65 < 211 > 426 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for SUMO - H 6 TEV - GLP - 1 K 28 R < 400 > 65 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 60 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 120 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg 180 gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat 240 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggtcatcat 300 catcaccacc acgaaaacct gtatttccag catgcggaag gcacctttac cagcgatgtg 360 agcagctatc tggaaggcca ggcggcgaaa gaatttattg cgtggctggt gcgtggccgc 420 ggctaa 426 < 210 > 66 < 211 > 144 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for H 6 TEV - hPTH 1 - 34 < 400 > 66 atgcatcatc atcaccacca cgaaaacctg tatttccagt ctgtgagtga aatacagctt 60 atgcataacc tgggaaaaca tctgaactcg atggagagag tagaatggct gcgtaagaag 120 ctgcaggatg tgcacaattt ttaa 144 < 210 > 67 < 211 > 531 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 97 - H 6 TEV - hPTH 1 - 34 < 400 > 67 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 60 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 120 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg 180 gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat 240 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggtcatcat 300 catcaccacc acgaaaacct gtatttccag catgcggaag gcacctttac cagcgatgtg 360 agcagctatc tggaaggcca ggcggcgaaa gaatttattg cgtggctggt gcgtggccgc 420 ggctaaagcg tgagcgaaat tcagctgatg cataacctgg gcaaacatct gaacagcatg 480 gaacgcgtgg aatggctgcg caaaaaactg caggatgtgc ataactttta a 531 < 210 > 68 < 211 > 435 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for SUMO - H 6 TEV - hPTH 1 - 34 < 400 > 68 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 60 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 120 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg 180 gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat 240 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggtcatcat 300 catcaccacc acgaaaacct gtatttccag agcgtgagcg aaattcagct gatgcataac 360 ctgggcaaac atctgaacag catggaacgc gtggaatggc tgcgcaaaaa actgcaggat 420 gtgcataact tttaa 435 < 210 > 69 < 211 > 141 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for H 6 TEV - GLP - 2 A 2 G < 400 > 69 atgcatcatc atcaccacca cgaaaacctg tatttccagc atggcgatgg cagctttagc 60 gatgaaatga acaccattct ggataacctg gcggcgcgcg attttattaa ctggctgatt 120 cagaccaaaa ttaccgatta a 141 < 210 > 70 < 211 > 429 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 97 - H 6 TEV - GLP - 2 A 2 G < 400 > 70 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa 60 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg 120 ctggctgtcg gcaatggccg tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa 180 gttggcgaca tcgttatttt caacgatggc tacggtgtga aatctgagaa gatcgacaat 240 gaagaagtgt tgatcatgtc cgaaagcgac attctggcaa ttgttgaagc gcatcatcat 300 caccaccacg aaaacctgta tttccagcat ggcgatggca gctttagcga tgaaatgaac 360 accattctgg ataacctggc ggcgcgcgat tttattaact ggctgattca gaccaaaatt 420 accgattaa 429 < 210 > 71 < 211 > 432 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for SUMO - H 6 TEV - GLP - 2 A 2 G < 400 > 71 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 60 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 120 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg 180 gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat 240 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggtcatcat 300 catcaccacc acgaaaacct gtatttccag catggcgatg gcagctttag cgatgaaatg 360 aacaccattc tggataacct ggcggcgcgc gattttatta actggctgat tcagaccaaa 420 attaccgatt aa 432 < 210 > 72 < 211 > 222 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for H 6 TEV - Ecallantide < 400 > 72 atgcatcatc atcaccacca cgaaaacctg tatttccagg aagcgatgca tagcttttgc 60 gcgtttaaag cggatgatgg cccgtgccgc gcggcgcatc cgcgctggtt ttttaacatt 120 tttacccgcc agtgcgaaga atttatttat ggcggctgcg aaggcaacca gaaccgcttt 180 gaaagcctgg aagaatgcaa aaaaatgtgc acccgcgatt aa 222 < 210 > 73 < 211 > 510 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 97 - H 6 TEV - Ecallantide < 400 > 73 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa 60 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg 120 ctggctgtcg gcaatggccg tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa 180 gttggcgaca tcgttatttt caacgatggc tacggtgtga aatctgagaa gatcgacaat 240 gaagaagtgt tgatcatgtc cgaaagcgac attctggcaa ttgttgaagc gcatcatcat 300 caccaccacg aaaacctgta tttccaggaa gcgatgcata gcttttgcgc gtttaaagcg 360 gatgatggcc cgtgccgcgc ggcgcatccg cgctggtttt ttaacatttt tacccgccag 420 tgcgaagaat ttatttatgg cggctgcgaa ggcaaccaga accgctttga aagcctggaa 480 gaatgcaaaa aaatgtgcac ccgcgattaa 510 < 210 > 74 < 211 > 513 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for SUMO - H 6 TEV - Ecallantide < 400 > 74 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 60 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 120 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg 180 gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat 240 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggtcatcat 300 catcaccacc acgaaaacct gtatttccag gaagcgatgc atagcttttg cgcgtttaaa 360 gcggatgatg gcccgtgccg cgcggcgcat ccgcgctggt tttttaacat ttttacccgc 420 cagtgcgaag aatttattta tggcggctgc gaaggcaacc agaaccgctt tgaaagcctg 480 gaagaatgca aaaaaatgtg cacccgcgat taa 513 < 210 > 75 < 211 > 138 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for H 6 TEV - Nesiritide < 400 > 75 atgcatcatc atcaccacca cgaaaacctg tatttccaga gcccgaaaat ggtgcagggc 60 agcggctgct ttggccgcaa aatggatcgc attagcagca gcagcggcct gggctgcaaa 120 gtgctgcgcc gccattaa 138 < 210 > 76 < 211 > 426 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 97 - H 6 TEV - Nesiritide < 400 > 76 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa 60 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg 120 ctggctgtcg gcaatggccg tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa 180 gttggcgaca tcgttatttt caacgatggc tacggtgtga aatctgagaa gatcgacaat 240 gaagaagtgt tgatcatgtc cgaaagcgac attctggcaa ttgttgaagc gcatcatcat 300 caccaccacg aaaacctgta tttccagagc ccgaaaatgg tgcagggcag cggctgcttt 360 ggccgcaaaa tggatcgcat tagcagcagc agcggcctgg gctgcaaagt gctgcgccgc 420 cattaa 426 < 210 > 77 < 211 > 429 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for SUMO - H 6 TEV - Nesiritide < 400 > 77 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 60 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 120 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg 180 gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat 240 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggtcatcat 300 catcaccacc acgaaaacct gtatttccag agcccgaaaa tggtgcaggg cagcggctgc 360 tttggccgca aaatggatcg cattagcagc agcagcggcc tgggctgcaa agtgctgcgc 420 cgccattaa 429 < 210 > 78 < 211 > 201 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for H 6 TEV - hEGF < 400 > 78 atgcatcatc atcaccacca cgaaaacctg tatttccaga actccgattc tgagtgccca 60 ctttcccacg atggatactg ccttcatgat ggcgtgtgca tgtacatcga ggctctcgat 120 aagtacgcct gcaactgcgt tgtgggttac attggagaga gatgccagta cagggatctc 180 aagtggtggg agcttaggta a 201 < 210 > 79 < 211 > 489 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 97 - H 6 TEV - hEGF < 400 > 79 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa 60 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg 120 ctggctgtcg gcaatggccg tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa 180 gttggcgaca tcgttatttt caacgatggc tacggtgtga aatctgagaa gatcgacaat 240 gaagaagtgt tgatcatgtc cgaaagcgac attctggcaa ttgttgaagc gcatcatcat 300 caccaccacg aaaacctgta tttccagaac tccgattctg agtgcccact ttcccacgat 360 ggatactgcc ttcatgatgg cgtgtgcatg tacatcgagg ctctcgataa gtacgcctgc 420 aactgcgttg tgggttacat tggagagaga tgccagtaca gggatctcaa gtggtgggag 480 cttaggtaa 489 < 210 > 80 < 211 > 492 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for SUMO - H 6 TEV - hEGF < 400 > 80 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 60 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 120 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg 180 gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat 240 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggtcatcat 300 catcaccacc acgaaaacct gtatttccag aactccgatt ctgagtgccc actttcccac 360 gatggatact gccttcatga tggcgtgtgc atgtacatcg aggctctcga taagtacgcc 420 tgcaactgcg ttgtgggtta cattggagag agatgccagt acagggatct caagtggtgg 480 gagcttaggt aa 492 < 210 > 81 < 211 > 537 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for H 6 TEV - INF - beta 1 b < 400 > 81 atgcatcatc atcaccacca cgaaaacctg tatttccaga gctataacct gctgggcttt 60 ctgcagcgca gcagcaactt tcagagccag aaactgctgt ggcagctgaa cggccgcctg 120 gaatattgcc tgaaagatcg catgaacttt gatattccgg aagaaattaa acagctgcag 180 cagtttcaga aagaagatgc ggcgctgacc atttatgaaa tgctgcagaa catttttgcg 240 atttttcgcc aggatagcag cagcaccggc tggaacgaaa ccattgtgga aaacctgctg 300 gcgaacgtgt atcatcagat taaccatctg aaaaccgtgc tggaagaaaa actggaaaaa 360 gaagatttta cccgcggcaa actgatgagc agcctgcatc tgaaacgcta ttatggccgc 420 attctgcatt atctgaaagc gaaagaatat agccattgcg cgtggaccat tgtgcgcgtg 480 gaaattctgc gcaactttta ttttattaac cgcctgaccg gctatctgcg caactaa 537 < 210 > 82 < 211 > 825 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 97 - H 6 TEV - INF - beta 1 b < 400 > 82 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa 60 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg 120 ctggctgtcg gcaatggccg tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa 180 gttggcgaca tcgttatttt caacgatggc tacggtgtga aatctgagaa gatcgacaat 240 gaagaagtgt tgatcatgtc cgaaagcgac attctggcaa ttgttgaagc gcatcatcat 300 caccaccacg aaaacctgta tttccagagc tataacctgc tgggctttct gcagcgcagc 360 agcaactttc agagccagaa actgctgtgg cagctgaacg gccgcctgga atattgcctg 420 aaagatcgca tgaactttga tattccggaa gaaattaaac agctgcagca gtttcagaaa 480 gaagatgcgg cgctgaccat ttatgaaatg ctgcagaaca tttttgcgat ttttcgccag 540 gatagcagca gcaccggctg gaacgaaacc attgtggaaa acctgctggc gaacgtgtat 600 catcagatta accatctgaa aaccgtgctg gaagaaaaac tggaaaaaga agattttacc 660 cgcggcaaac tgatgagcag cctgcatctg aaacgctatt atggccgcat tctgcattat 720 ctgaaagcga aagaatatag ccattgcgcg tggaccattg tgcgcgtgga aattctgcgc 780 aacttttatt ttattaaccg cctgaccggc tatctgcgca actaa 825 < 210 > 83 < 211 > 828 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for SUMO - H 6 TEV - INF - beta 1 b < 400 > 83 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 60 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 120 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg 180 gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat 240 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggtcatcat 300 catcaccacc acgaaaacct gtatttccag agctataacc tgctgggctt tctgcagcgc 360 agcagcaact ttcagagcca gaaactgctg tggcagctga acggccgcct ggaatattgc 420 ctgaaagatc gcatgaactt tgatattccg gaagaaatta aacagctgca gcagtttcag 480 aaagaagatg cggcgctgac catttatgaa atgctgcaga acatttttgc gatttttcgc 540 caggatagca gcagcaccgg ctggaacgaa accattgtgg aaaacctgct ggcgaacgtg 600 tatcatcaga ttaaccatct gaaaaccgtg ctggaagaaa aactggaaaa agaagatttt 660 acccgcggca aactgatgag cagcctgcat ctgaaacgct attatggccg cattctgcat 720 tatctgaaag cgaaagaata tagccattgc gcgtggacca ttgtgcgcgt ggaaattctg 780 cgcaactttt attttattaa ccgcctgacc ggctatctgc gcaactaa 828 < 210 > 84 < 211 > 612 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for H 6 TEV - hGH < 400 > 84 catcatcatc accaccacga aaacctgtat ttccagtttc caaccattcc actgtctcgc 60 ctgtttgata atgcaatgct gcgtgcacat cgtctgcatc agctggcatt tgatacctat 120 caggaatttg aagaagcata tattccaaaa gaacagaaat attctttcct gcaaaatcca 180 cagacctctc tgtgtttttc tgaatctatt ccaaccccat ccaatcgtga agagacccaa 240 cagaaatcta atctggaact gctgcgtatc tctctgttac tgattcagtc ttggctggaa 300 ccagtgcagt tcctgcgttc tgtgttcgca aattctctgg tttatggtgc atctgattct 360 aatgtttatg atctgctgaa agatttagag gaaggaattc agaccctgat gggtcgtctg 420 gaagatggtt ctccacgtac cggtcagatt tttaaacaga cctattctaa atttgatacc 480 aattctcata atgacgatgc actgctgaaa aactatggtc tgctgtactg ctttcgtaaa 540 gatatggata aagttgaaac ctttctgcgt attgttcagt gtcgttctgt tgaaggttct 600 tgtggttttt aa 612 < 210 > 85 < 211 > 903 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 97 - H 6 TEV - hGH < 400 > 85 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa 60 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg 120 ctggctgtcg gcaatggccg tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa 180 gttggcgaca tcgttatttt caacgatggc tacggtgtga aatctgagaa gatcgacaat 240 gaagaagtgt tgatcatgtc cgaaagcgac attctggcaa ttgttgaagc gcatcatcat 300 caccaccacg aaaacctgta tttccagttt ccaaccattc cactgtctcg cctgtttgat 360 aatgcaatgc tgcgtgcaca tcgtctgcat cagctggcat ttgataccta tcaggaattt 420 gaagaagcat atattccaaa agaacagaaa tattctttcc tgcaaaatcc acagacctct 480 ctgtgttttt ctgaatctat tccaacccca tccaatcgtg aagagaccca acagaaatct 540 aatctggaac tgctgcgtat ctctctgtta ctgattcagt cttggctgga accagtgcag 600 ttcctgcgtt ctgtgttcgc aaattctctg gtttatggtg catctgattc taatgtttat 660 gatctgctga aagatttaga ggaaggaatt cagaccctga tgggtcgtct ggaagatggt 720 tctccacgta ccggtcagat ttttaaacag acctattcta aatttgatac caattctcat 780 aatgacgatg cactgctgaa aaactatggt ctgctgtact gctttcgtaa agatatggat 840 aaagttgaaa cctttctgcg tattgttcag tgtcgttctg ttgaaggttc ttgtggtttt 900 taa 903 < 210 > 86 < 211 > 906 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for SUMO - H 6 TEV - hGH < 400 > 86 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 60 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 120 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg 180 gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat 240 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggtcatcat 300 catcaccacc acgaaaacct gtatttccag tttccaacca ttccactgtc tcgcctgttt 360 gataatgcaa tgctgcgtgc acatcgtctg catcagctgg catttgatac ctatcaggaa 420 tttgaagaag catatattcc aaaagaacag aaatattctt tcctgcaaaa tccacagacc 480 tctctgtgtt tttctgaatc tattccaacc ccatccaatc gtgaagagac ccaacagaaa 540 tctaatctgg aactgctgcg tatctctctg ttactgattc agtcttggct ggaaccagtg 600 cagttcctgc gttctgtgtt cgcaaattct ctggtttatg gtgcatctga ttctaatgtt 660 tatgatctgc tgaaagattt agaggaagga attcagaccc tgatgggtcg tctggaagat 720 ggttctccac gtaccggtca gatttttaaa cagacctatt ctaaatttga taccaattct 780 cataatgacg atgcactgct gaaaaactat ggtctgctgt actgctttcg taaagatatg 840 gataaagttg aaacctttct gcgtattgtt cagtgtcgtt ctgttgaagg ttcttgtggt 900 ttttaa 906 < 210 > 87 < 211 > 300 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for H 6 TEV - INSG < 400 > 87 atgcatcatc atcaccacca cgaaaacctg tatttccagt ttgtgaacca gcatctgtgc 60 ggcagccatc tggtggaagc gctgtatctg gtgtgcggcg aacgcggctt tttttatacc 120 ccgaaaaccc gccgcgaagc ggaagatctg caggtgggcc aggtggaact gggcggcggc 180 ccgggcgcgg gcagcctgca gccgctggcg ctggaaggca gcctgcagaa acgcggcatt 240 gtggaacagt gctgcaccag catttgcagc ctgtatcagc tggaaaacta ttgcggctaa 300 300 < 210 > 88 < 211 > 588 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for PG 97 - H 6 TEV - INSG < 400 > 88 atgaatattc gtccattgca tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa 60 tctgctggcg gcatcgttct gaccggctct gcagcggcta aatccacccg cggcgaagtg 120 ctggctgtcg gcaatggccg tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa 180 gttggcgaca tcgttatttt caacgatggc tacggtgtga aatctgagaa gatcgacaat 240 gaagaagtgt tgatcatgtc cgaaagcgac attctggcaa ttgttgaagc gcatcatcat 300 caccaccacg aaaacctgta tttccagttt gtgaaccagc atctgtgcgg cagccatctg 360 gtggaagcgc tgtatctggt gtgcggcgaa cgcggctttt tttatacccc gaaaacccgc 420 cgcgaagcgg aagatctgca ggtgggccag gtggaactgg gcggcggccc gggcgcgggc 480 agcctgcagc cgctggcgct ggaaggcagc ctgcagaaac gcggcattgt ggaacagtgc 540 tgcaccagca tttgcagcct gtatcagctg gaaaactatt gcggctaa 588 < 210 > 89 < 211 > 591 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > nucleotide sequence for SUMO - H 6 TEV - INSG < 400 > 89 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 60 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 120 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg 180 gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat 240 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggtcatcat 300 catcaccacc acgaaaacct gtatttccag tttgtgaacc agcatctgtg cggcagccat 360 ctggtggaag cgctgtatct ggtgtgcggc gaacgcggct ttttttatac cccgaaaacc 420 cgccgcgaag cggaagatct gcaggtgggc caggtggaac tgggcggcgg cccgggcgcg 480 ggcagcctgc agccgctggc gctggaaggc agcctgcaga aacgcggcat tgtggaacag 540 tgctgcacca gcatttgcag cctgtatcag ctggaaaact attgcggcta a 591