< 110 > Korea University Industry and Academy Cooperation Foundation < 120 > USE OF INHIBITORS OF LEUKOTRIENE B 4 RECEPTOR BLT 2 FOR TREATING HUMAN CANCERS < 160 > 12 < 170 > KopatentIn 1 . 71 < 210 > 1 < 211 > 2798 < 212 > DNA < 213 > homo sapiens < 400 > 1 aaactggccc tggccctgaa ccaaatacct tgaaccctcg taaactccat accctgaccc 60 ccttgttttg gatataccca ggtagaacaa ctctctctca ctgtctgttg tgaggatacg 120 ctgtagccca ctcattaagt acattctcct aataaatgct ttggactgat caccctgcca 180 gtcttttgtc ttgggcaatc tatacttttc tcagaggttc ccaaggccta ctgaagggac 240 ttaacatact cttaatggct ttcctctctc ttgttttacc ttatgccctc acttcctgag 300 ttaacctccc aaatacagga tcacctgtac ccaagccctt agctcaagaa tacaggatca 360 cctgtaccca agcccttagc tcaagctctg ctttggaaga acccaaacta agacagtgct 420 cctggtgccc tccccaagca acctcaagtt ctggctgtta cttgagcaga ggcctttctt 480 ttcccttccc ccagctctat ccatctgcca ggcccccctc aaatctcttc atttccaagt 540 tttgcttgac ttttccaaga ggagagggct gcttcttagt atgtccctac tcatcctttc 600 ctttcttgtc ttgtatcctg gtgcagcctg gtaatggggc ctcttcatgg ttgtgtgtca 660 tgactcccta accattatgc ctccatgcat cccctgttcc tcctggaacc tagcaccatg 720 ccttacatgg aaaagctgtc attgacagcc cggtgagagc cctgagggtg gagtgactgg 780 ggcagggcct gaggcaagag gtgggaggag gtaggaggcc aggggctcag ccggaccagg 840 agactggaaa caggcaagga taaggcaggt gggggactga gttgtttggg tcacctctgc 900 aggccagaga gaccaggcaa catacacact gcagaaggtg ggctgggagg attggggcca 960 gagctggggg agggatgaga acagaagcag gaccaggatt cagcagagtc ctcctatttc 1020 cttccaccac cagggaatct tactgcccca cttcagcttg tgctgtttcc tggcaaggca 1080 ggctctcaca tgcctggacg cctgggtgcg ttggtgatgg gaaggagcag ggtgagggag 1140 gggccccagg agaggcccag gatgagcctc atcttgtccc tccccattct tgtcttaccc 1200 tctgcaaatg tgataggcac aggacaggag taggcacctc gcctactgct gcttaacctt 1260 tcagcttctc caggccccca atcctgcttg ctcccagctt ggtaagtaga tctgtgcacg 1320 tccctttaca ccccaccatc cagttttgcc cagatgtgct agaatggggc tggacaaaga 1380 aggaggggcc agactagagg agtggtggta gagatagtga cagcctgggg tgaggacttt 1440 atgcctgttt accactgagc tctgggaagg aggccaggag tggggcaggt caactgactg 1500 ggagcagggg atctgggttc caagaaggag ttgtgtttga ggtggggtct gggtcctcgt 1560 ggaagtcagg actcccaggc agaaaagagg caggctgcag ggaagtaagg aggaggcatg 1620 gcaccttctc atcgggcatc acaggtgggg ttttgcccca cccctgaacg ccctctgtgg 1680 cgccttccac ccacctgtag gcccagaagg atgtcggtct gctaccgtcc cccagggaac 1740 gagacactgc tgagctggaa gacttcgcgg gccacaggca cagccttcct gctgctggcg 1800 gcgctgctgg ggctgcctgg caacggcttc gtggtgtgga gcttggcggg ctggcggcct 1860 gcacgggggc gaccgctggc ggccacgctt gtgctgcacc tggcgctggc cgacggcgcg 1920 gtgctgctgc tcacgccgct ctttgtggcc ttcctgaccc ggcaggcctg gccgctgggc 1980 caggcgggct gcaaggcggt gtactacgtg tgcgcgctca gcatgtacgc cagcgtgctg 2040 ctcaccggcc tgctcagcct gcagcgctgc ctcgcagtca cccgcccctt cctggcgcct 2100 cggctgcgca gcccggccct ggcccgccgc ctgctgctgg cggtctggct ggccgccctg 2160 ttgctcgccg tcccggccgc cgtctaccgc cacctgtgga gggaccgcgt atgccagctg 2220 tgccacccgt cgccggtcca cgccgccgcc cacctgagcc tggagactct gaccgctttc 2280 gtgcttcctt tcgggctgat gctcggctgc tacagcgtga cgctggcacg gctgcggggc 2340 gcccgctggg gctccgggcg gcacggggcg cgggtgggcc ggctggtgag cgccatcgtg 2400 cttgccttcg gcttgctctg ggccccctac cacgcagtca accttctgca ggcggtcgca 2460 gcgctggctc caccggaagg ggccttggcg aagctgggcg gagccggcca ggcggcgcga 2520 gcgggaacta cggccttggc cttcttcagt tctagcgtca acccggtgct ctacgtcttc 2580 accgctggag atctgctgcc ccgggcaggt ccccgtttcc tcacgcggct cttcgaaggc 2640 tctggggagg cccgaggggg cggccgctct agggaaggga ccatggagct ccgaactacc 2700 cctcagctga aagtggtggg gcagggccgc ggcaatggag acccgggggg tgggatggag 2760 aaggacggtc cggaatggga cctttgacag cagaccct 2798 < 210 > 2 < 211 > 1170 < 212 > DNA < 213 > homo sapiens < 220 > < 221 > CDS < 222 > ( 1 ) . .( 1167 ) < 400 > 2 atg gca cct tct cat cgg gca tca cag gtg ggg ttt tgc ccc acc cct 48 Met Ala Pro Ser His Arg Ala Ser Gln Val Gly Phe Cys Pro Thr Pro 1 5 10 15 gaa cgc cct ctg tgg cgc ctt cca ccc acc tgt agg ccc aga agg atg 96 Glu Arg Pro Leu Trp Arg Leu Pro Pro Thr Cys Arg Pro Arg Arg Met 20 25 30 tcg gtc tgc tac cgt ccc cca ggg aac gag aca ctg ctg agc tgg aag 144 Ser Val Cys Tyr Arg Pro Pro Gly Asn Glu Thr Leu Leu Ser Trp Lys 35 40 45 act tcg cgg gcc aca ggc aca gcc ttc ctg ctg ctg gcg gcg ctg ctg 192 Thr Ser Arg Ala Thr Gly Thr Ala Phe Leu Leu Leu Ala Ala Leu Leu 50 55 60 ggg ctg cct ggc aac ggc ttc gtg gtg tgg agc ttg gcg ggc tgg cgg 240 Gly Leu Pro Gly Asn Gly Phe Val Val Trp Ser Leu Ala Gly Trp Arg 65 70 75 80 cct gca cgg ggg cga ccg ctg gcg gcc acg ctt gtg ctg cac ctg gcg 288 Pro Ala Arg Gly Arg Pro Leu Ala Ala Thr Leu Val Leu His Leu Ala 85 90 95 ctg gcc gac ggc gcg gtg ctg ctg ctc acg ccg ctc ttt gtg gcc ttc 336 Leu Ala Asp Gly Ala Val Leu Leu Leu Thr Pro Leu Phe Val Ala Phe 100 105 110 ctg acc cgg cag gcc tgg ccg ctg ggc cag gcg ggc tgc aag gcg gtg 384 Leu Thr Arg Gln Ala Trp Pro Leu Gly Gln Ala Gly Cys Lys Ala Val 115 120 125 tac tac gtg tgc gcg ctc agc atg tac gcc agc gtg ctg ctc acc ggc 432 Tyr Tyr Val Cys Ala Leu Ser Met Tyr Ala Ser Val Leu Leu Thr Gly 130 135 140 ctg ctc agc ctg cag cgc tgc ctc gca gtc acc cgc ccc ttc ctg gcg 480 Leu Leu Ser Leu Gln Arg Cys Leu Ala Val Thr Arg Pro Phe Leu Ala 145 150 155 160 cct cgg ctg cgc agc ccg gcc ctg gcc cgc cgc ctg ctg ctg gcg gtc 528 Pro Arg Leu Arg Ser Pro Ala Leu Ala Arg Arg Leu Leu Leu Ala Val 165 170 175 tgg ctg gcc gcc ctg ttg ctc gcc gtc ccg gcc gcc gtc tac cgc cac 576 Trp Leu Ala Ala Leu Leu Leu Ala Val Pro Ala Ala Val Tyr Arg His 180 185 190 ctg tgg agg gac cgc gta tgc cag ctg tgc cac ccg tcg ccg gtc cac 624 Leu Trp Arg Asp Arg Val Cys Gln Leu Cys His Pro Ser Pro Val His 195 200 205 gcc gcc gcc cac ctg agc ctg gag act ctg acc gct ttc gtg ctt cct 672 Ala Ala Ala His Leu Ser Leu Glu Thr Leu Thr Ala Phe Val Leu Pro 210 215 220 ttc ggg ctg atg ctc ggc tgc tac agc gtg acg ctg gca cgg ctg cgg 720 Phe Gly Leu Met Leu Gly Cys Tyr Ser Val Thr Leu Ala Arg Leu Arg 225 230 235 240 ggc gcc cgc tgg ggc tcc ggg cgg cac ggg gcg cgg gtg ggc cgg ctg 768 Gly Ala Arg Trp Gly Ser Gly Arg His Gly Ala Arg Val Gly Arg Leu 245 250 255 gtg agc gcc atc gtg ctt gcc ttc ggc ttg ctc tgg gcc ccc tac cac 816 Val Ser Ala Ile Val Leu Ala Phe Gly Leu Leu Trp Ala Pro Tyr His 260 265 270 gca gtc aac ctt ctg cag gcg gtc gca gcg ctg gct cca ccg gaa ggg 864 Ala Val Asn Leu Leu Gln Ala Val Ala Ala Leu Ala Pro Pro Glu Gly 275 280 285 gcc ttg gcg aag ctg ggc gga gcc ggc cag gcg gcg cga gcg gga act 912 Ala Leu Ala Lys Leu Gly Gly Ala Gly Gln Ala Ala Arg Ala Gly Thr 290 295 300 acg gcc ttg gcc ttc ttc agt tct agc gtc aac ccg gtg ctc tac gtc 960 Thr Ala Leu Ala Phe Phe Ser Ser Ser Val Asn Pro Val Leu Tyr Val 305 310 315 320 ttc acc gct gga gat ctg ctg ccc cgg gca ggt ccc cgt ttc ctc acg 1008 Phe Thr Ala Gly Asp Leu Leu Pro Arg Ala Gly Pro Arg Phe Leu Thr 325 330 335 cgg ctc ttc gaa ggc tct ggg gag gcc cga ggg ggc ggc cgc tct agg 1056 Arg Leu Phe Glu Gly Ser Gly Glu Ala Arg Gly Gly Gly Arg Ser Arg 340 345 350 gaa ggg acc atg gag ctc cga act acc cct cag ctg aaa gtg gtg ggg 1104 Glu Gly Thr Met Glu Leu Arg Thr Thr Pro Gln Leu Lys Val Val Gly 355 360 365 cag ggc cgc ggc aat gga gac ccg ggg ggt ggg atg gag aag gac ggt 1152 Gln Gly Arg Gly Asn Gly Asp Pro Gly Gly Gly Met Glu Lys Asp Gly 370 375 380 ccg gaa tgg gac ctt tga 1170 Pro Glu Trp Asp Leu 385 < 210 > 3 < 211 > 389 < 212 > PRT < 213 > homo sapiens < 400 > 3 Met Ala Pro Ser His Arg Ala Ser Gln Val Gly Phe Cys Pro Thr Pro 1 5 10 15 Glu Arg Pro Leu Trp Arg Leu Pro Pro Thr Cys Arg Pro Arg Arg Met 20 25 30 Ser Val Cys Tyr Arg Pro Pro Gly Asn Glu Thr Leu Leu Ser Trp Lys 35 40 45 Thr Ser Arg Ala Thr Gly Thr Ala Phe Leu Leu Leu Ala Ala Leu Leu 50 55 60 Gly Leu Pro Gly Asn Gly Phe Val Val Trp Ser Leu Ala Gly Trp Arg 65 70 75 80 Pro Ala Arg Gly Arg Pro Leu Ala Ala Thr Leu Val Leu His Leu Ala 85 90 95 Leu Ala Asp Gly Ala Val Leu Leu Leu Thr Pro Leu Phe Val Ala Phe 100 105 110 Leu Thr Arg Gln Ala Trp Pro Leu Gly Gln Ala Gly Cys Lys Ala Val 115 120 125 Tyr Tyr Val Cys Ala Leu Ser Met Tyr Ala Ser Val Leu Leu Thr Gly 130 135 140 Leu Leu Ser Leu Gln Arg Cys Leu Ala Val Thr Arg Pro Phe Leu Ala 145 150 155 160 Pro Arg Leu Arg Ser Pro Ala Leu Ala Arg Arg Leu Leu Leu Ala Val 165 170 175 Trp Leu Ala Ala Leu Leu Leu Ala Val Pro Ala Ala Val Tyr Arg His 180 185 190 Leu Trp Arg Asp Arg Val Cys Gln Leu Cys His Pro Ser Pro Val His 195 200 205 Ala Ala Ala His Leu Ser Leu Glu Thr Leu Thr Ala Phe Val Leu Pro 210 215 220 Phe Gly Leu Met Leu Gly Cys Tyr Ser Val Thr Leu Ala Arg Leu Arg 225 230 235 240 Gly Ala Arg Trp Gly Ser Gly Arg His Gly Ala Arg Val Gly Arg Leu 245 250 255 Val Ser Ala Ile Val Leu Ala Phe Gly Leu Leu Trp Ala Pro Tyr His 260 265 270 Ala Val Asn Leu Leu Gln Ala Val Ala Ala Leu Ala Pro Pro Glu Gly 275 280 285 Ala Leu Ala Lys Leu Gly Gly Ala Gly Gln Ala Ala Arg Ala Gly Thr 290 295 300 Thr Ala Leu Ala Phe Phe Ser Ser Ser Val Asn Pro Val Leu Tyr Val 305 310 315 320 Phe Thr Ala Gly Asp Leu Leu Pro Arg Ala Gly Pro Arg Phe Leu Thr 325 330 335 Arg Leu Phe Glu Gly Ser Gly Glu Ala Arg Gly Gly Gly Arg Ser Arg 340 345 350 Glu Gly Thr Met Glu Leu Arg Thr Thr Pro Gln Leu Lys Val Val Gly 355 360 365 Gln Gly Arg Gly Asn Gly Asp Pro Gly Gly Gly Met Glu Lys Asp Gly 370 375 380 Pro Glu Trp Asp Leu 385 < 210 > 4 < 211 > 1077 < 212 > DNA < 213 > homo sapiens < 220 > < 221 > CDS < 222 > ( 1 ) . .( 1074 ) < 400 > 4 atg tcg gtc tgc tac cgt ccc cca ggg aac gag aca ctg ctg agc tgg 48 Met Ser Val Cys Tyr Arg Pro Pro Gly Asn Glu Thr Leu Leu Ser Trp 1 5 10 15 aag act tcg cgg gcc aca ggc aca gcc ttc ctg ctg ctg gcg gcg ctg 96 Lys Thr Ser Arg Ala Thr Gly Thr Ala Phe Leu Leu Leu Ala Ala Leu 20 25 30 ctg ggg ctg cct ggc aac ggc ttc gtg gtg tgg agc ttg gcg ggc tgg 144 Leu Gly Leu Pro Gly Asn Gly Phe Val Val Trp Ser Leu Ala Gly Trp 35 40 45 cgg cct gca cgg ggg cga ccg ctg gcg gcc acg ctt gtg ctg cac ctg 192 Arg Pro Ala Arg Gly Arg Pro Leu Ala Ala Thr Leu Val Leu His Leu 50 55 60 gcg ctg gcc gac ggc gcg gtg ctg ctg ctc acg ccg ctc ttt gtg gcc 240 Ala Leu Ala Asp Gly Ala Val Leu Leu Leu Thr Pro Leu Phe Val Ala 65 70 75 80 ttc ctg acc cgg cag gcc tgg ccg ctg ggc cag gcg ggc tgc aag gcg 288 Phe Leu Thr Arg Gln Ala Trp Pro Leu Gly Gln Ala Gly Cys Lys Ala 85 90 95 gtg tac tac gtg tgc gcg ctc agc atg tac gcc agc gtg ctg ctc acc 336 Val Tyr Tyr Val Cys Ala Leu Ser Met Tyr Ala Ser Val Leu Leu Thr 100 105 110 ggc ctg ctc agc ctg cag cgc tgc ctc gca gtc acc cgc ccc ttc ctg 384 Gly Leu Leu Ser Leu Gln Arg Cys Leu Ala Val Thr Arg Pro Phe Leu 115 120 125 gcg cct cgg ctg cgc agc ccg gcc ctg gcc cgc cgc ctg ctg ctg gcg 432 Ala Pro Arg Leu Arg Ser Pro Ala Leu Ala Arg Arg Leu Leu Leu Ala 130 135 140 gtc tgg ctg gcc gcc ctg ttg ctc gcc gtc ccg gcc gcc gtc tac cgc 480 Val Trp Leu Ala Ala Leu Leu Leu Ala Val Pro Ala Ala Val Tyr Arg 145 150 155 160 cac ctg tgg agg gac cgc gta tgc cag ctg tgc cac ccg tcg ccg gtc 528 His Leu Trp Arg Asp Arg Val Cys Gln Leu Cys His Pro Ser Pro Val 165 170 175 cac gcc gcc gcc cac ctg agc ctg gag act ctg acc gct ttc gtg ctt 576 His Ala Ala Ala His Leu Ser Leu Glu Thr Leu Thr Ala Phe Val Leu 180 185 190 cct ttc ggg ctg atg ctc ggc tgc tac agc gtg acg ctg gca cgg ctg 624 Pro Phe Gly Leu Met Leu Gly Cys Tyr Ser Val Thr Leu Ala Arg Leu 195 200 205 cgg ggc gcc cgc tgg ggc tcc ggg cgg cac ggg gcg cgg gtg ggc cgg 672 Arg Gly Ala Arg Trp Gly Ser Gly Arg His Gly Ala Arg Val Gly Arg 210 215 220 ctg gtg agc gcc atc gtg ctt gcc ttc ggc ttg ctc tgg gcc ccc tac 720 Leu Val Ser Ala Ile Val Leu Ala Phe Gly Leu Leu Trp Ala Pro Tyr 225 230 235 240 cac gca gtc aac ctt ctg cag gcg gtc gca gcg ctg gct cca ccg gaa 768 His Ala Val Asn Leu Leu Gln Ala Val Ala Ala Leu Ala Pro Pro Glu 245 250 255 ggg gcc ttg gcg aag ctg ggc gga gcc ggc cag gcg gcg cga gcg gga 816 Gly Ala Leu Ala Lys Leu Gly Gly Ala Gly Gln Ala Ala Arg Ala Gly 260 265 270 act acg gcc ttg gcc ttc ttc agt tct agc gtc aac ccg gtg ctc tac 864 Thr Thr Ala Leu Ala Phe Phe Ser Ser Ser Val Asn Pro Val Leu Tyr 275 280 285 gtc ttc acc gct gga gat ctg ctg ccc cgg gca ggt ccc cgt ttc ctc 912 Val Phe Thr Ala Gly Asp Leu Leu Pro Arg Ala Gly Pro Arg Phe Leu 290 295 300 acg cgg ctc ttc gaa ggc tct ggg gag gcc cga ggg ggc ggc cgc tct 960 Thr Arg Leu Phe Glu Gly Ser Gly Glu Ala Arg Gly Gly Gly Arg Ser 305 310 315 320 agg gaa ggg acc atg gag ctc cga act acc cct cag ctg aaa gtg gtg 1008 Arg Glu Gly Thr Met Glu Leu Arg Thr Thr Pro Gln Leu Lys Val Val 325 330 335 ggg cag ggc cgc ggc aat gga gac ccg ggg ggt ggg atg gag aag gac 1056 Gly Gln Gly Arg Gly Asn Gly Asp Pro Gly Gly Gly Met Glu Lys Asp 340 345 350 ggt ccg gaa tgg gac ctt tga 1077 Gly Pro Glu Trp Asp Leu 355 < 210 > 5 < 211 > 358 < 212 > PRT < 213 > homo sapiens < 400 > 5 Met Ser Val Cys Tyr Arg Pro Pro Gly Asn Glu Thr Leu Leu Ser Trp 1 5 10 15 Lys Thr Ser Arg Ala Thr Gly Thr Ala Phe Leu Leu Leu Ala Ala Leu 20 25 30 Leu Gly Leu Pro Gly Asn Gly Phe Val Val Trp Ser Leu Ala Gly Trp 35 40 45 Arg Pro Ala Arg Gly Arg Pro Leu Ala Ala Thr Leu Val Leu His Leu 50 55 60 Ala Leu Ala Asp Gly Ala Val Leu Leu Leu Thr Pro Leu Phe Val Ala 65 70 75 80 Phe Leu Thr Arg Gln Ala Trp Pro Leu Gly Gln Ala Gly Cys Lys Ala 85 90 95 Val Tyr Tyr Val Cys Ala Leu Ser Met Tyr Ala Ser Val Leu Leu Thr 100 105 110 Gly Leu Leu Ser Leu Gln Arg Cys Leu Ala Val Thr Arg Pro Phe Leu 115 120 125 Ala Pro Arg Leu Arg Ser Pro Ala Leu Ala Arg Arg Leu Leu Leu Ala 130 135 140 Val Trp Leu Ala Ala Leu Leu Leu Ala Val Pro Ala Ala Val Tyr Arg 145 150 155 160 His Leu Trp Arg Asp Arg Val Cys Gln Leu Cys His Pro Ser Pro Val 165 170 175 His Ala Ala Ala His Leu Ser Leu Glu Thr Leu Thr Ala Phe Val Leu 180 185 190 Pro Phe Gly Leu Met Leu Gly Cys Tyr Ser Val Thr Leu Ala Arg Leu 195 200 205 Arg Gly Ala Arg Trp Gly Ser Gly Arg His Gly Ala Arg Val Gly Arg 210 215 220 Leu Val Ser Ala Ile Val Leu Ala Phe Gly Leu Leu Trp Ala Pro Tyr 225 230 235 240 His Ala Val Asn Leu Leu Gln Ala Val Ala Ala Leu Ala Pro Pro Glu 245 250 255 Gly Ala Leu Ala Lys Leu Gly Gly Ala Gly Gln Ala Ala Arg Ala Gly 260 265 270 Thr Thr Ala Leu Ala Phe Phe Ser Ser Ser Val Asn Pro Val Leu Tyr 275 280 285 Val Phe Thr Ala Gly Asp Leu Leu Pro Arg Ala Gly Pro Arg Phe Leu 290 295 300 Thr Arg Leu Phe Glu Gly Ser Gly Glu Ala Arg Gly Gly Gly Arg Ser 305 310 315 320 Arg Glu Gly Thr Met Glu Leu Arg Thr Thr Pro Gln Leu Lys Val Val 325 330 335 Gly Gln Gly Arg Gly Asn Gly Asp Pro Gly Gly Gly Met Glu Lys Asp 340 345 350 Gly Pro Glu Trp Asp Leu 355 < 210 > 6 < 211 > 15 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > hBLT 2 anti - sense sequence < 400 > 6 cagcagtgtc tcgtt 15 < 210 > 7 < 211 > 21 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > hBLT 2 siRNA sense sequence < 400 > 7 gaaggauguc ggucugcuau u 21 < 210 > 8 < 211 > 21 < 212 > RNA < 213 > Artificial Sequence < 220 > < 223 > hBLT 2 siRNA antisense sequence < 400 > 8 uagcagaccg acauccuucu u 21 < 210 > 9 < 211 > 25 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > forward primer for hBLT 2 < 400 > 9 agcctggaga ctctgaccgc tttcg 25 < 210 > 10 < 211 > 25 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > reverse primer for hBLT 2 < 400 > 10 gacgtagagc accgggttga cgcta 25 < 210 > 11 < 211 > 20 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > forward primer for re - hBLT 2 < 400 > 11 tctcatcggg catcacaggt 20 < 210 > 12 < 211 > 20 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > reverse primer for re - hBLT 2 < 400 > 12 ccaagctcca caccacgaag 20