- Input titer : 2 . 32 ㎕ of library stock left from step 6 in 500 ㎕ of SB → 2 . 32 ㎕ in 500 ㎕ of SB → 2 . 32 ㎕ in 500 ㎕ of SB → 1 ㎕ in 100 ㎕ of E . coli cell → 100 ㎕ spreading → 다음 날 colony 수 counting ( n ) → Input = n * 10 - 10