기기 는 centrifuge ( Sigma Co . , U . S . A . ) , deep - freezer ( Sanyo Co . , Japan ) , vortex mixer ( Vision scientific Co . , Korea ) , plate shaker ( Lab - Line Co . , U . S . A . ) , ELISA reader ( Molecular Devices Co . , U . S . A . ) , optical microscope ( Carl ZEISS Co . , Germany ) , Light Microscope ( Carl Zeiss , Co . , Germany ) , Mini - Protean tetra Cell ( BIO - RAD Co . , Japan ) , Nanodrop ( Thermo fisher , U . S . A . ) , Alpha Cycler 1 PCRmax ( PCRmax , U . K . ) real time PCR ( Qiagen , Germany ) 등 을 사용 하 였 다 .