본 개시 내용 의 실시 는 달리 표시 되 지 않 는 한 , 관련 기술 분야 의 기술 수준 내 에 있 는 분자 생물학 ( 재조합 기술 포함 ) , 미생물학 , 세포 생물학 , 생화학 및 면역학 의 통상 적 인 기술 을 이용 할 것 이 다 . 이러 한 기술 은 문헌 , 예컨대 하 기 문헌 에 완전히 설명 되 어 있 다 [ Molecular Cloning : A Laboratory Manual , second edition ( Sambrook , et al . , 1989 ) Cold Spring Harbor Press ; Oligonucleotide Synthesis ( M . J . Gait , ed . 1984 ) ; Methods in Molecular Biology , Humana Press ; Cell Biology : A Laboratory Notebook ( J . E . Cellis , ed . , 1989 ) Academic Press ; Animal Cell Culture ( R . I . Freshney , ed . 1987 ) ; Introuction to Cell and Tissue Culture ( J . P . Mather and P . E . Roberts , 1998 ) Plenum Press ; Cell and Tissue Culture : Laboratory Procedures ( A . Doyle , J . B . Griffiths , and D . G . Newell , eds . 1993 - 8 ) J . Wiley and Sons ; Methods in Enzymology ( Academic Press , Inc . ); Handbook of Experimental Immunology ( D . M . Weir and C . C . Blackwell , eds . ) : Gene Transfer Vectors for Mammalian Cells ( J . M . Miller and M . P . Calos , eds . , 1987 ) ; Current Protocols in Molecular Biology ( F . M . Ausubel , et al . eds . 1987 ) ; PCR : The Polymerase Chain Reaction , ( Mullis , et al . , eds . 1994 ) ; Current Protocols in Immunology ( J . E . Coligan et al . , eds . , 1991 ) ; Short Protocols in Molecular Biology ( Wiley and Sons , 1999 ) ; Immunobiology ( C . A . Janeway and P . Travers , 1997 ) ; Antibodies ( P . Finch , 1997 ) ; Antibodies : a practice approach ( D . Catty . , ed . , IRL Press , 1988 - 1989 ) ; Monoclonal antibodies : a practical approach ( P . Shepherd and C . Dean , eds . , Oxford University Press , 2000 ) ; Using antibodies : a laboratory manual ( E . Harlow and D . Lane ( Cold Spring Harbor Laboratory Press , 1999 ) ; The Antibodies ( M . Zanetti and J . D . Capra , eds . Harwood Academic Publishers , 1995 ) ; DNA Cloning : A practical Approach , Volumes I and II ( D . N . Glover ed . 1985 ) ; Nucleic Acid Hybridization ( B . D . Hames & S . J . Higgins eds . ( 1985 ≫; Transcription and Translation ( B . D . Hames & S . J . Higgins , eds . ( 1984 ≫; Animal Cell Culture ( R . I . Freshney , ed . ( 1986 ≫; Immobilized Cells and Enzymes ( lRL Press , ( 1986 ≫; 및 B . Perbal , A practical Guide To Molecular Cloning ( 1984 ) ; F . M . Ausubel et al . ( eds . ) ] .