구매 논문 장바구니 고객 센터 
 한국 미생물 생 명공 학회 ( 구 한국 산업 미생물 학회 ) 
 19 권 1 호 
 - 주제 : 자연 과학 분야 > 생물 
 - 간기 : 월간 
 - 해외 등재 : SCI / SCOPUS 
 - ISSN : 1017 - 7825 
 수록 범위 : 19 권 1 호 ( 2009 ) 
 Inhella inkyongensis gen . nov . , sp . nov . , a New Freshwater Bacterium in the Order Burkholderiales 
 ( Jae Ho Song ) , ( Hyun Myung Oh ) , ( Jung Sook Lee ) , ( Seung Buhm Woo ) , ( Jang Cheon Cho ) 
 한국 미생물 생 명공 학회 ( 구 한국 산업 미생물 학회 ) | Journal of Microbiology and Biotechnology 19 권 1 호 , 2009 pp . 5 - 10 ( 총 6 pages ) 
 Inhella inkyongensis Sphaerotilus - Leptothrix group freshwater novel genus 16 S rRNA gene 
 Functional Analysis of MCNA , a Gene Encoding a Catalytic Subunit of Calcineurin , in the Rice Blast Fungus Magnaporthe oryzae 
 ( Jin Hee Choi ) , ( Yang Seon Kim ) , ( Yong Hwan Lee ) 
 한국 미생물 생 명공 학회 ( 구 한국 산업 미생물 학회 ) | Journal of Microbiology and Biotechnology 19 권 1 호 , 2009 pp . 11 - 16 ( 총 6 pages ) 
 Calcineurin Magnaporthe oryzae calmodulin protein phosphatase sense / antisense appressorium 
 Magnaporthe oryzae , the causal agent of rice blast , forms a specialized infection structure , called an appressorium , which is crucial for penetration and infection of the host plant . Pharmacological data suggest that calcium / calmodulin - dependent signaling is involved in appressorium formation in this fungus . To understand the role of the calcium / calmodulin - activated protein phosphatase on appressorium formation at the molecular level , MCNA , a gene encoding the catalytic subunit of calcineurin , was functionally characterized in M . oryzae . Transformants expressing sense / antisense RNA of MCNA exhibited significant reductions in mycelial growth , conidiation , appressorium formation , and pathogenicity . cDNA of MCNA functionally complemented a calcineurin disruptant strain ( cmp 1 : : LEU 2 cmp 2 : : HIS 3 ) of Saccharomyces cerevisiae . These data suggest that calcineurin A plays important roles in signal transduction pathways involved in the infection - related morphogenesis and pathogenicity of M . oryzae . 
 Thiosulfate Oxidation and Mixotrophic Growth of Methylobacterium goesingense and Methylobacterium fujisawaense 
 ( R . Anandham ) , ( P . Indiragandhi ) , ( M . Madhaiyan ) , ( Jong Bae Chung ) , ( Kyoung Yul Ryu ) , ( Hyeong Jin Jee ) , ( Tong Min Sa ) 
 한국 미생물 생 명공 학회 ( 구 한국 산업 미생물 학회 ) | Journal of Microbiology and Biotechnology 19 권 1 호 , 2009 pp . 17 - 22 ( 총 6 pages ) 
 Thiosulfate oxidation mixotrophic growth rhodanese sulfite oxidase soxB S 4 I PSO 
 The mixotrophic growth with methanol plus thiosulfate was examined in nutrient - limited mixotrophic condition for Methylobacterium goesingense CBMB 5 and Methylobacterium fujisawaense CBMB 37 . Thiosulfate oxidation increased the growth and protein yield in mixotrophic medium that contained 150 mM methanol and 20 mM sodium thiosulfate , at 144 h . Respirometric study revealed that thiosulfate was the most preferable reduced inorganic sulfur source , followed by sulfite and sulfur . M . goesingense CBMB 5 and M . fujisawaense CBMB 37 oxidized thiosulfate directly to sulfate , and intermediate products of thiosulfate oxidation such as polythionates , sulfite , and sulfur were not detected in spent medium and they did not yield positive amplification for tested soxB primers . Enzymes of thiosulfate oxidation such as rhodanese and sulfite oxidase activities were detected in cell - free extracts of M . goesingense CBMB 5 , and M . fujisawaense CBMB 37 , and thiosulfate oxidase ( tetrathionate synthase ) activity was not observed . It indicated that both the organisms use the " non - S 4 intermediate " sulfur oxidation pathway for thiosulfate oxidation . It is concluded from this study that M . goesingense CBMB 5 , and M . fujisawaense CBMB 37 exhibited mixotrophic metabolism in medium containing methanol plus thiosulfate and that thiosulfate oxidation and the presence of a " Paracoccus sulfur oxidation " ( PSO ) pathway in methylotrophic bacteria are species dependant . 
 Investigation of the Central Carbon Metabolism of Sorangium cellulosum : Metabolic Network Reconstruction and Quantification of Pathway Fluxes 
 ( Christoph J . Bolten ) , ( Elmar Heinzle ) , ( Rolf Muller ) , ( Christoph Wittmann ) 
 한국 미생물 생 명공 학회 ( 구 한국 산업 미생물 학회 ) | Journal of Microbiology and Biotechnology 19 권 1 호 , 2009 pp . 23 - 36 ( 총 14 pages ) 
 Myxobacteria primary metabolism flux NADPH maintenance 
 In the present work , the metabolic network of primary metabolism of the slow - growing myxobacterium Sorangium cellulosum was reconstructed from the annotated genome sequence of the type strain So ce 56 . During growth on glucose as the carbon source and asparagine as the nitrogen source , So ce 56 showed a very low growth rate of 0 . 23 d - 1 , equivalent to a doubling time of 3 days . Based on a complete stoichiometric and isotopomer model of the central metabolism , 13 C metabolic flux analysis was carried out for growth with glucose as carbon and asparagine as nitrogen sources . Normalized to the uptake flux for glucose ( 100 %), cells recruited glycolysis ( 51 %) and the pentose phosphate pathway ( 48 %) as major catabolic pathways . The Entner - Doudoroff pathway and glyoxylate shunt were not active . A high flux through the TCA cycle ( 118 %) enabled a strong formation of ATP , but cells revealed a rather low yield for biomass . Inspection of fluxes linked to energy metabolism revealed that S . cellulosum utilized only 10 % of the ATP formed for growth , whereas 90 % is required for maintenance . This explains the apparent discrepancy between the relatively low biomass yield and the high flux through the energy - delivering TCA cycle . The total flux of NADPH supply ( 216 %) was higher than the demand for anabolism ( 156 %), indicating additional reactions for balancing of NADPH . The cells further exhibited a highly active metabolic cycle , interconverting C 3 and C 4 metabolites of glycolysis and the TCA cycle . The present work provides the first insight into fluxes of the primary metabolism of myxobacteria , especially for future investigation on the supply of cofactors , building blocks , and energy in myxobacteria , producing natural compounds of biotechnological interest . 
 Energy Generation Coupled to Azoreduction by Membranous Vesicles from Shewanella decolorationis S 12 
 ( Yi Guo Hong ) , ( Jun Guo ) , ( Guo Ping Sun ) 
 한국 미생물 생 명공 학회 ( 구 한국 산업 미생물 학회 ) | Journal of Microbiology and Biotechnology 19 권 1 호 , 2009 pp . 37 - 41 ( 총 5 pages ) 
 Membranous vesicles azoreduction energy conservation Shewanella decolorationis S 12 
 Previous studies have demonstrated that Shewanella decolorationis S 12 can grow on the azo compound amaranth as the sole electron acceptor . Thus , to explore the mechanism of energy generation in this metabolism , membranous vesicles ( MVs ) were prepared and the mechanism of energy generation was investigated . The membrane , which was fragmentized during preparation , automatically formed vesicles ranging from 37 . 5 - 112 . 5 nm in diameter under electron micrograph observation . Energy was conserved when coupling the azoreduction by the MVs of an azo compound or Fe ( III ) as the sole electron acceptor with H 2 , formate , or lactate as the electron donor . The amaranth reduction by the vesicles was found to be inhibited by specific respiratory inhibitors , including Cu 2 + ions , dicumarol , stigmatellin , and metyrapone , indicating that the azoreduction was indeed a respiration reaction . This finding was further confirmed by the fact that the ATP synthesis was repressed by the ATPase inhibitor N , N `- dicyclohexylcarbodiimide ( DCCD ) . Therefore , this study offers solid evidence of a mechanism of microbial dissimilatory azoreduction on a subcell level . 
 Diverse Antibacterial Activity of Pectobacterium carotovorum subsp . carotovorum Isolated in Korea 
 ( Eun Jung Roh ) , ( Seung Don Lee ) , ( Yong Hoon Lee ) , ( Dong Su Ra ) , ( Jae Hyuk Choi ) , ( Eun Pyo Moon ) , ( Sung Gi Heu ) 
 한국 미생물 생 명공 학회 ( 구 한국 산업 미생물 학회 ) | Journal of Microbiology and Biotechnology 19 권 1 호 , 2009 pp . 42 - 50 ( 총 9 pages ) 
 Soft rot bacteriocin antibacterial substance carotovoricin carocin S 1 
 Fifty - four Pectobacterium carotovorum subsp . Carotovorum strains isolated in Korea were characterized by a spectrum of antibacterial activities against 7 indicator strains chosen to represent various regions and host plants . All P . carotovorum subsp . carotovorum isolates tested could be grouped into 4 classes depending on the pattern of antibacterial substance production . All tested strains had DNA fragment ( s ) homologous to the genes encoding carotovoricin and 21 of them had genes homologous to DNA invertase . Sixteen strains had genes homologous to the genes encoding carocin S 1 . Several isolates produced antibacterial substances active against strains in Brenneria , Pantoea , and Pectobacterium genera that belonged formerly to the genus Erwinia . Strains in Pseudomonas or Xanthomonas sp . were not sensitive to the antibacterial substances produced by P . carotovorum subsp . carotovorum , except for X . albilineans that was sensitive to antibacterial substances produced by most strains in P . carotovorum subsp . carotovorum and P . betavasculorum KACC 10056 . These results demonstrated the diverse patterns of antibacterial substance production and the possibility of the existence of new antibacterial substance ( s ) produced by P . carotovorum subsp . carotovorum isolated in Korea . 
 LC - MS / MS Profiling - Based Secondary Metabolite Screening of Myxococcus xanthus 
 ( Ji Young Kim ) , ( Jung Nam Choi ) , ( Pil Kim ) , ( Dai Eun Sok ) , ( Soo Wan Nam ) , ( Choong Hwan Lee ) 
 한국 미생물 생 명공 학회 ( 구 한국 산업 미생물 학회 ) | Journal of Microbiology and Biotechnology 19 권 1 호 , 2009 pp . 51 - 54 ( 총 4 pages ) 
 Metabolite profiling LC - MS / MS Myxococcus xanthus myxalamid 
 Myxobacteria , Gram - negative soil bacteria , are a well - known producer of bioactive secondary metabolites . Therefore , this study presents a methodological approach for the high - throughput screening of secondary metabolites from 4 wild - type Myxococcus xanthus strains . First , electrospray ionization mass spectrometry ( ESI - MS ) was performed using extracellular crude extracts . As a result , 22 metabolite peaks were detected , and the metabolite profiling was then conducted using the m / z value , retention time , and MS / MS fragmentation pattern analyses . Among the peaks , one unknown compound peak was identified as analogous to the myxalamid A , B , and C series . An analysis of the tandem mass spectrometric fragmentation patterns and HR - MS identified myxalamid K as a new compound derived from M . xanthus . In conclusion , LC - MS / MS - based chemical screening of diverse secondary metabolites would appear to be an effective approach for discovering unknown microbial secondary metabolites . 
 Characteristics of B - Cell - Specific Growth Substance Produced by Bacillus Licheniformis E 1 
 ( Joo Young Kim ) , ( Kun Sub Chung ) , ( Jeon Han Park ) , ( Yi Sub Kwak ) , ( Bong Ki Lee ) 
 한국 미생물 생 명공 학회 ( 구 한국 산업 미생물 학회 ) | Journal of Microbiology and Biotechnology 19 권 1 호 , 2009 pp . 55 - 64 ( 총 10 pages ) 
 Adjuvant Bacillus Licheniformis E 1 B cell - specific growth substance BGS immunomodulator 
 A B cell - specific growth substance ( BGS ) was isolated from the slime layer of Bacillus licheniformis E 1 . Unlike LPS , the BGS was not affected by polymixin B , an inhibitor of LPS , or by TLR 4 , and resulted in the growth of B cells . When BALB / c mice were treated with the BGS , the B cell population was found to increase in both the bone marrow and the spleen , with a marked increase after 24 h in the bone marrow and after 48 h in the spleen . When using antibodies to B cell lineage - restricted surface molecules to analyze the B cell population changes resulting from treatment with the BGS , an increase in immature B cells ( IgM + and AA 4 . 1 +) and mature B cells ( IgM + and IgD +) was found in the bone marrow 24 h after treatment with the BGS , whereas a decrease in mature B cells and increase in IgG + B cells were found in the spleen . When the BGS and OVA antigen were injected into the peritoneal cavity of BALB / c mice , this resulted in a high OVA - specific antibody titer in the sera , similar to that induced by aluminum hydroxide . Therefore , it is anticipated that the mass production of the BGS by B . licheniformis E 1 could be used for studies of B cells in immunology , and contribute to the development of a new adjuvant for vaccine manufacture . 
 A New Strategy to Improve the Efficiency and Sustainability of Candida parapsilosis Catalyzing Deracemization of ( R , S ) - 1 - Phenyl - 1 , 2 - Ethanediol Under Non - Growing Conditions : Increase of NADPH Availability 
 ( Yao Nie ) , ( Yan Xu ) , ( Qing Sen Hu ) , ( Rong Xiao ) 
 한국 미생물 생 명공 학회 ( 구 한국 산업 미생물 학회 ) | Journal of Microbiology and Biotechnology 19 권 1 호 , 2009 pp . 65 - 71 ( 총 7 pages ) 
 Candida parapsilosis deracemization NADPH availability pentose sustainability 
 Microbial oxidoreductive systems have been widely used in asymmetric syntheses of optically active alcohols . However , when reused in multi - batch reaction , the catalytic efficiency and sustainability of non - growing cells usually decreased because of continuous consumption of required cofactors during the reaction process . A novel method for NADPH regeneration in cells was proposed by using pentose metabolism in microorganisms . Addition of D - xylose , L - arabinose , or D - ribose to the reaction significantly improved the conversion efficiency of deracemization of racemic 1 - phenyl - 1 , 2 - ethanediol to ( S ) - isomer by Candida parapsilosis cells already used once , which afforded the product with high optical purity over 97 % e . e . in high yield over 85 % under an increased substrate concentration of 15 g / l . Compared with reactions without xylose , xylose added to multi - batch reactions had no influence on the activity of the enzyme catalyzing the key step in deracemization , but performed a promoting effect on the recovery of the metabolic activity of the non - growing cells with its consumption in each batch . The detection of activities of xylose reductase and xylitol dehydrogenase from cell - free extract of C . parapsilosis made xylose metabolism feasible in cells , and the depression of the pentose phosphate pathway inhibitor to this reaction further indicated that xylose facilitated the NADPH - required deracemization through the pentose phosphate pathway in C . parapsilosis . moreover , by investigating the cofactor pool , the xylose addition in reaction batches giving more NADPH , compared with those without xylose , suggested that the higher catalytic efficiency and sustainability of C . parapsilosis non - growing cells had resulted from xylose metabolism recycling NADPH for the deracemization . 
 Improving the Productivity of Recombinant Protein in Escherichia coli Under Thermal Stress by Coexpressing GroELS Chaperone System 
 ( So Yeon Kim ) , ( Niraikulam Ayyadurai ) , ( Mi Ae Heo ) , ( Sung Hoon Park ) , ( Yong Joo Jeong ) , ( Sun Gu Lee ) 
 한국 미생물 생 명공 학회 ( 구 한국 산업 미생물 학회 ) | Journal of Microbiology and Biotechnology 19 권 1 호 , 2009 pp . 72 - 77 ( 총 6 pages ) 
 GroELS thermotolerance recombinant protein Escherichia coli 
 Here , we demonstrate that the overexpression of the GroELS chaperone system , which assists the folding of intracellular proteins and prevents aggregation of its biological targets , can enhance the thermotolerance of Escherichia coli strains and facilitate the production of recombinant protein under thermal stress . The overexpression of GroELS led to an about 2 - fold higher growth rate of E . coli XL - 1 blue than control at 45 ℃ and induced the growth of the strain even at 50 ℃, although the growth was not sustained in the second - round culture . The effect of GroELS overexpression was also effective on other E . coli strains such as JM 109 , DH 5 α , and BL 21 . Finally , we have shown that coexpression of GroELS allows us to produce recombinant protein even at 50 ℃, a temperature at which the protein production based on E . coli is not efficient . This study indicates that the employment of the GroELS overexpression system can expand the range of environmental conditions for E . coli .