Either you are a troll or so thick headed none can argue with you . Either way , hope I never see you in person . <| sep |> I regularly clone libraries of 384 genes . Everything is pooled and manipulated in parallel . I suppose for your application you have to be able to physically separate them out at the end ? You could barcode each variant and do a dCas 9 targeted pulldown . How are you making the genes PCR or synthesis ? <| sep |> Well yeah , high platinum is like the top 80 %, of course good players will be masters . <| sep |> Nobody is forced to read the Daily Mail , but some are addicted to perusing it in order to find something that offends them . <| sep |> Ahhh , I knew they weren ' t copying Jake and Amir but I wasn ' t sure what it was actually from . Being British I ' ve never seen Seinfeld haha