SEQUENCE LISTING < 110 > Eligo Bioscience , Institut Pasteur < 120 > OPTIMIZED VECTOR FOR DELIVERY IN MICROBIAL POPULATIONS < 130 > B 2437 PC < 160 > 233 < 170 > PatentIn version 3 . 5 < 210 > 1 < 211 > 14 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 1 cacnnnnnnn ctgg 14 < 210 > 2 < 211 > 13 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 2 aacnnnnnng tgc 13 < 210 > 3 < 211 > 11 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 7 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 11 ) . .( 11 ) < 400 > 3 cacnnnngta y 11 < 210 > 4 < 211 > 11 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restricition site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 7 ) < 223 > n is a , c , g , or t < 400 > 4 aacnnnnctt t 11 < 210 > 5 < 211 > 14 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restricition site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 5 ccannnnnnn cttc 14 < 210 > 6 < 211 > 15 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 11 ) . .( 11 ) < 223 > r is A or G < 220 > < 221 > misc _ feature < 222 > ( 13 ) . .( 13 ) < 223 > r is A or G < 400 > 6 tacnnnnnnn rtrtc 15 < 210 > 7 < 211 > 14 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 7 gagnnnnnnn gtca 14 < 210 > 8 < 211 > 15 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 8 tgannnnnnn ntgct 15 < 210 > 9 < 211 > 13 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 9 agcannnnnn tga 13 < 210 > 10 < 211 > 13 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 10 tgannnnnnc ttc 13 < 210 > 11 < 211 > 12 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 11 ) . .( 11 ) < 223 > y is c or t < 400 > 11 gagnnnnngt ty 12 < 210 > 12 < 211 > 13 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 12 gatgnnnnnn tac 13 < 210 > 13 < 211 > 13 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is a or g < 400 > 13 gaannnnnnr tcg 13 < 210 > 14 < 211 > 13 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1 ) < 223 > r is a or g < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 14 rtcannnnnn ctc 13 < 210 > 15 < 211 > 13 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 8 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 9 ) . .( 9 ) < 223 > r is a or g < 220 > < 221 > misc _ feature < 222 > ( 11 ) . .( 11 ) < 223 > d is a , g or t < 400 > 15 gnagnnnnrt dca 13 < 210 > 16 < 211 > 14 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 11 ) . .( 11 ) < 223 > r is a or g < 400 > 16 gaannnnnnn rtcg 14 < 210 > 17 < 211 > 15 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 17 ggannnnnnn natgc 15 < 210 > 18 < 211 > 11 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 18 gagnnnnntc c 11 < 210 > 19 < 211 > 14 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 19 cacnnnnnnn gttg 14 < 210 > 20 < 211 > 14 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1 ) < 223 > y is c or t < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 14 ) . .( 14 ) < 223 > y is c or t < 400 > 20 ytcannnnnn gtty 14 < 210 > 21 < 211 > 12 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 21 gatgnnnnnc tg 12 < 210 > 22 < 211 > 13 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is c or t < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 13 ) . .( 13 ) < 223 > y is c or t < 400 > 22 ccaynnnnng tty 13 < 210 > 23 < 211 > 16 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1 ) < 223 > r is a or g < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 12 ) < 223 > n is a , c , g , or t < 400 > 23 rtcannnnnn nngtgg 16 < 210 > 24 < 211 > 14 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 24 gaannnnnnn taaa 14 < 210 > 25 < 211 > 14 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 11 ) . .( 11 ) < 223 > r is a or g < 400 > 25 tcannnnnnn rttc 14 < 210 > 26 < 211 > 12 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 26 gacnnnnnng tc 12 < 210 > 27 < 211 > 16 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 12 ) < 223 > n is a , c , g , or t < 400 > 27 ttcannnnnn nnctgg 16 < 210 > 28 < 211 > 14 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 14 ) . .( 14 ) < 223 > y is c or t < 400 > 28 ttannnnnnn gtcy 14 < 210 > 29 < 211 > 14 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Resztriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 11 ) . .( 11 ) < 223 > r is a or g < 400 > 29 ccannnnnnn rtgc 14 < 210 > 30 < 211 > 15 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 30 ccannnnnnn ntgaa 15 < 210 > 31 < 211 > 14 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 31 gagnnnnnnn atgc 14 < 210 > 32 < 211 > 12 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 32 cagnnnnnnc gt 12 < 210 > 33 < 211 > 12 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 33 gatgnnnnng gc 12 < 210 > 34 < 211 > 7 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 5 ) < 223 > b is c , g or t < 400 > 34 gaaabcc 7 < 210 > 35 < 211 > 5 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > w is a or t < 400 > 35 ccwgg 5 < 210 > 36 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 36 ggtctc 6 < 210 > 37 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 37 ctgcag 6 < 210 > 38 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 38 gccggc 6 < 210 > 39 < 211 > 7 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1 ) < 223 > r is a or g < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 7 ) . .( 7 ) < 223 > y is c or t < 400 > 39 rggnccy 7 < 210 > 40 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 40 gtcgac 6 < 210 > 41 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 41 gcgcgc 6 < 210 > 42 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1 ) < 223 > r is a or g < 220 > < 221 > misc _ feature < 222 > ( 6 ) . .( 6 ) < 223 > y is c or T < 400 > 42 rccggy 6 < 210 > 43 < 211 > 5 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > n is a , c , g , or t < 400 > 43 ccngg 5 < 210 > 44 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 44 aagctt 6 < 210 > 45 < 211 > 7 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > n is a , c , g , or t < 400 > 45 cancatc 7 < 210 > 46 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > r is a or g < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 5 ) < 223 > y is c or t < 400 > 46 grcgyc 6 < 210 > 47 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > y is c or t < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 5 ) < 223 > r is a or g < 400 > 47 cycgrg 6 < 210 > 48 < 211 > 5 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > n is a , c , g , or t < 400 > 48 gcngc 5 < 210 > 49 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1 ) < 223 > y is c or t < 220 > < 221 > misc _ feature < 222 > ( 6 ) . .( 6 ) < 223 > r is a or g < 400 > 49 yggccr 6 < 210 > 50 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction Site < 400 > 50 ccgcgg 6 < 210 > 51 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > r is a or g < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 5 ) < 223 > y is c or t < 400 > 51 grgcyc 6 < 210 > 52 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 52 ctgaag 6 < 210 > 53 < 211 > 5 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > w is a or t < 400 > 53 ggwcc 5 < 210 > 54 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 54 tggcca 6 < 210 > 55 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 4 ) < 223 > w is a or t < 400 > 55 ccwwgg 6 < 210 > 56 < 211 > 5 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > n is a , c , g , or t < 400 > 56 ggncc 5 < 210 > 57 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 57 gagctc 6 < 210 > 58 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 58 ggtacc 6 < 210 > 59 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 59 ggcgcc 6 < 210 > 60 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is c or t < 400 > 60 accyac 6 < 210 > 61 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 61 gaattc 6 < 210 > 62 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 62 gatatc 6 < 210 > 63 < 211 > 7 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > n is a , c , g , or t < 400 > 63 cctnagg 7 < 210 > 64 < 211 > 7 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > n is a , c , g , or t < 400 > 64 ggtnacc 7 < 210 > 65 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 65 atgcat 6 < 210 > 66 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > y is c or t < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > r is a or g < 400 > 66 ggyrcc 6 < 210 > 67 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 67 aggcct 6 < 210 > 68 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 68 ctcaat 6 < 210 > 69 < 211 > 5 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > w is a or t < 400 > 69 gcwgc 5 < 210 > 70 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 70 tcgcga 6 < 210 > 71 < 211 > 11 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 71 cctnnnnnag g 11 < 210 > 72 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 72 accacc 6 < 210 > 73 < 211 > 5 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 73 cacag 5 < 210 > 74 < 211 > 5 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 74 gaacc 5 < 210 > 75 < 211 > 5 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 75 gagac 5 < 210 > 76 < 211 > 6 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 76 cagcag 6 < 210 > 77 < 211 > 5 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction enzyme < 400 > 77 agacc 5 < 210 > 78 < 211 > 5 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Restriction site < 400 > 78 ccgag 5 < 210 > 79 < 211 > 6 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > Restriction site < 400 > 79 gatcag 6 < 210 > 80 < 211 > 15 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 80 ggannnnnnn natgc 15 < 210 > 81 < 211 > 11 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 81 gagnnnnnta c 11 < 210 > 82 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 82 gcannnnnnn ntgc 14 < 210 > 83 < 211 > 11 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 83 gacnnnnntg a 11 < 210 > 84 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 84 ggtannnnnn tcg 13 < 210 > 85 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is g or a < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is g or a < 220 > < 221 > misc _ feature < 222 > ( 13 ) . .( 13 ) < 223 > y is t or c < 400 > 85 gagnnnnnnr tayg 14 < 210 > 86 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 9 ) . .( 9 ) < 223 > r is g or a < 400 > 86 cccnnnnnrt ag 12 < 210 > 87 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 87 ggyannnnnn tcg 13 < 210 > 88 < 211 > 15 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 88 ccannnnnnn ntgag 15 < 210 > 89 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 89 gcannnnnnt taa 13 < 210 > 90 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > r is g or a < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 90 craannnnnn ntgc 14 < 210 > 91 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 11 ) . .( 11 ) < 223 > r is g or a < 400 > 91 craannnnnn ntgc 14 < 210 > 92 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 92 aagnnnnnnc atc 13 < 210 > 93 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 93 cagnnnnnnc gt 12 < 210 > 94 < 211 > 15 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 94 gaaynnnnnn nctgg 15 < 210 > 95 < 211 > 15 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 95 cgannnnnnn ntgcc 15 < 210 > 96 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 96 acgnnnnngt tg 12 < 210 > 97 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 3 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 97 cyyannnnnn cttc 14 < 210 > 98 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 11 ) . .( 11 ) < 223 > r is g or a < 400 > 98 cacnnnnnnn rtgt 14 < 210 > 99 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 99 cccannnnnn tcg 13 < 210 > 100 < 211 > 11 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 100 aggnnnnnga t 11 < 210 > 101 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 101 ccaynnnnnn tgt 13 < 210 > 102 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 102 ccaynnnnnn gta 13 < 210 > 103 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 103 aagnnnnnnc atc 13 < 210 > 104 < 211 > 11 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 6 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 7 ) . .( 7 ) < 223 > r is g or a < 400 > 104 gatnnnnrta c 11 < 210 > 105 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 9 ) . .( 9 ) < 223 > r is g or a < 400 > 105 gcannnnnrt ta 12 < 210 > 106 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 13 ) < 223 > r is g or a < 400 > 106 raacnnnnnn rtta 14 < 210 > 107 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 107 agtnnnnnnr ttg 13 < 210 > 108 < 211 > 10 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 108 gagnnnnngt 10 < 210 > 109 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 109 gaaynnnnng tc 12 < 210 > 110 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 110 gannnnnntg cg 12 < 210 > 111 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 111 ttagnnnnnn ttc 13 < 210 > 112 < 211 > 11 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 112 gatgnnnntg t 11 < 210 > 113 < 211 > 11 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 9 ) . .( 9 ) < 223 > r is g or a < 400 > 113 gagnnnnnrt g 11 < 210 > 114 < 211 > 11 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 114 cacnnnnnta c 11 < 210 > 115 < 211 > 15 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > r is g or a < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 12 ) < 223 > n is a , c , g , or t < 400 > 115 craannnnnn nnctt 15 < 210 > 116 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 116 tgannnnnnn tatc 14 < 210 > 117 < 211 > 16 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > r is g or a < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 13 ) < 223 > n is a , c , g , or t < 400 > 117 craannnnnn nnnttc 16 < 210 > 118 < 211 > 15 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > r is g or a < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 12 ) < 223 > n is a , c , g , or t < 400 > 118 craannnnnn nnctg 15 < 210 > 119 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 119 cacnnnnnnn ctg 13 < 210 > 120 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 120 cacnnnnnnn nttc 14 < 210 > 121 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 121 cacnnnnnnn ctt 13 < 210 > 122 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 122 tttannnnnn ngtt 14 < 210 > 123 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 12 ) . .( 12 ) < 223 > y is t or c < 400 > 123 ctannnnngt ty 12 < 210 > 124 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 124 gaynnnnnng tt 12 < 210 > 125 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 125 cccannnnnc tg 12 < 210 > 126 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 126 tccannnnnn cgt 13 < 210 > 127 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 9 ) . .( 9 ) < 223 > r is g or a < 400 > 127 acgnnnnnrt gt 12 < 210 > 128 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 128 atgnnnnnnc ctc 13 < 210 > 129 < 211 > 15 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > b is g or c or t < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 129 tcabnnnnnn ntcca 15 < 210 > 130 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 130 gacnnnnnng atc 13 < 210 > 131 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 131 aggnnnnntt ca 12 < 210 > 132 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 132 gaagnnnnnt ac 12 < 210 > 133 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 133 ccaynnnnnt taa 13 < 210 > 134 < 211 > 15 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 11 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 12 ) . .( 12 ) < 223 > r is g or a < 400 > 134 cncannnnnn nrtgt 15 < 210 > 135 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is g or a < 220 > < 221 > misc _ feature < 222 > ( 13 ) . .( 13 ) < 223 > n is a , c , g , or t < 400 > 135 ccannnnnnr ttnc 14 < 210 > 136 < 211 > 15 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 136 acaynnnnnn nttgg 15 < 210 > 137 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 137 gatannnnnn ntgc 14 < 210 > 138 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 138 gccnnnnngt tg 12 < 210 > 139 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 139 catcnnnnnn tcc 13 < 210 > 140 < 211 > 17 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1 ) < 223 > r is g or a < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 13 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 15 ) . .( 15 ) < 223 > r is g or a < 400 > 140 rtcannnnnn nnntrgg 17 < 210 > 141 < 211 > 11 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 141 attcnnnnct g 11 < 210 > 142 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 142 taagnnnnnn ntgg 14 < 210 > 143 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is g or a < 400 > 143 gagnnnnnnr tgc 13 < 210 > 144 < 211 > 11 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 144 cccnnnnnct c 11 < 210 > 145 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 145 agcnnnnnnt aca 13 < 210 > 146 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 146 ccannnnnnn ctta 14 < 210 > 147 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 147 gcaynnnnnn ctc 13 < 210 > 148 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 148 cannnnnnnt aaag 14 < 210 > 149 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 9 ) . .( 9 ) < 223 > r is g or a < 400 > 149 tagnnnnnrt gaa 13 < 210 > 150 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 150 attynnnnnc ttc 13 < 210 > 151 < 211 > 12 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 151 aygnnnnnnc tg 12 < 210 > 152 < 211 > 13 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is g or a < 400 > 152 gaannnnnnr tgc 13 < 210 > 153 < 211 > 14 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > b is g , c or t < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 13 ) . .( 13 ) < 223 > n is a , c , g , or t < 400 > 153 tacbnnnnnn gtng 14 < 210 > 154 < 211 > 11 < 212 > DNA < 213 > artificial sequence < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 154 gatgnnnntg t 11 < 210 > 155 < 211 > 11 < 212 > DNA < 213 > artificial sequenec < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 155 gacnnnnngg t 11 < 210 > 156 < 211 > 15 < 212 > DNA < 213 > artificial sequenec < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 11 ) . .( 11 ) < 223 > r is g or a < 400 > 156 ggannnnnnn rtggc 15 < 210 > 157 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 157 ctannnnnnn ntgt 14 < 210 > 158 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 158 acannnnnnn ntag 14 < 210 > 159 < 211 > 11 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 159 ccannnnnnt c 11 < 210 > 160 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 13 ) . .( 13 ) < 223 > y is t or c < 400 > 160 gannnnnnnt ayg 13 < 210 > 161 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1 ) < 223 > r is g or a < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 13 ) . .( 13 ) < 223 > y is t or c < 400 > 161 rtaynnnnnr tay 13 < 210 > 162 < 211 > 11 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 162 aagnnnnnct t 11 < 210 > 163 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 163 aagnnnnnnt aaag 14 < 210 > 164 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restrictioon site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 164 ccannnnnnt tt 12 < 210 > 165 < 211 > 11 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 165 cagnnnnnga t 11 < 210 > 166 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 166 aagnnnnnct cc 12 < 210 > 167 < 211 > 11 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 7 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 8 ) . .( 8 ) < 223 > r is g or a < 400 > 167 gagnnnnrta a 11 < 210 > 168 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 168 ggannnnnnc ttt 13 < 210 > 169 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 14 ) . .( 14 ) < 223 > y is t or c < 400 > 169 ttannnnnnn gtcy 14 < 210 > 170 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 170 gaagnnnnnn ntcc 14 < 210 > 171 < 211 > 10 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 7 ) < 223 > n is a , c , g , or t < 400 > 171 cagnnnnctg 10 < 210 > 172 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is g or a < 400 > 172 acannnnnnr tgg 13 < 210 > 173 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 173 gcaynnnnnn catc 14 < 210 > 174 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 13 ) . .( 13 ) < 223 > y is t or c < 400 > 174 ataynnnnnc tay 13 < 210 > 175 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restrction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is g or a < 400 > 175 gaaynnnnnr tac 13 < 210 > 176 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 176 ccannnnnnt tga 13 < 210 > 177 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is a or g < 400 > 177 ggannnnnnr taa 13 < 210 > 178 < 211 > 11 < 212 > DNA < 213 > artificial < 220 > < 223 > restrction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 9 ) . .( 9 ) < 223 > r is g or a < 400 > 178 gaynnnnnrt c 11 < 210 > 179 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 179 cggannnnnn ttc 13 < 210 > 180 < 211 > 11 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 180 acannnnngt g 11 < 210 > 181 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 181 gtannnnnnn ctc 13 < 210 > 182 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 182 gcannnnnnt taa 13 < 210 > 183 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 183 acgnnnnngt tg 12 < 210 > 184 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 184 aagnnnnngt tc 12 < 210 > 185 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 185 caynnnnnnt ca 12 < 210 > 186 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > h is a or c or t < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 186 caahnnnnnn cttc 14 < 210 > 187 < 211 > 15 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 187 gcannnnnnn ngtgg 15 < 210 > 188 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 188 tcagnnnnnn tgc 13 < 210 > 189 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 189 caacnnnnnc tt 12 < 210 > 190 < 211 > 16 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 11 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 12 ) . .( 12 ) < 223 > r is g ro a < 220 > < 221 > misc _ feature < 222 > ( 14 ) . .( 14 ) < 223 > d is a or g or t < 400 > 190 gaaynnnnnn nrtdcc 16 < 210 > 191 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 191 cgaannnnnt ga 12 < 210 > 192 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 192 gacnnnnnnc tgg 13 < 210 > 193 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 193 tgannnnnnn tggg 14 < 210 > 194 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 194 gagnnnnnnt ta 12 < 210 > 195 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 195 gagnnnnnnt gg 12 < 210 > 196 < 211 > 16 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 3 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 6 ) . .( 12 ) < 223 > n is a , c , g , or t < 400 > 196 gnngannnnn nntggg 16 < 210 > 197 < 211 > 11 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > k is g or t < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 197 taaknnnngt c 11 < 210 > 198 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 198 cgcannnnnc tg 12 < 210 > 199 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 199 ccannnnnnn tggg 14 < 210 > 200 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 200 agynnnnnnc ttc 13 < 210 > 201 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 201 ccannnnnnn ctc 13 < 210 > 202 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > Y IS T OR C < 220 > < 221 > misc _ feature < 222 > ( 13 ) . .( 13 ) < 223 > n is a , c , g , or t < 400 > 202 ccannnnnnr ttnc 14 < 210 > 203 < 211 > 16 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1 ) < 223 > r is g or a < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 12 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 13 ) . .( 13 ) < 223 > r is g or a < 400 > 203 rgaannnnnn nnrtga 16 < 210 > 204 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is g or a < 400 > 204 agtnnnnnnr tgc 13 < 210 > 205 < 211 > 15 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > h is a or c or t < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 11 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 14 ) . .( 14 ) < 223 > d is a or g or t < 400 > 205 ghtannnnnn ntadc 15 < 210 > 206 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 206 gyaynnnnnc ttg 13 < 210 > 207 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 14 ) . .( 14 ) < 223 > y is t or c < 400 > 207 tagnnnnnnc ttgy 14 < 210 > 208 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 208 ccaynnnnng ct 12 < 210 > 209 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is g or a < 400 > 209 ctannnnnnr tgaa 14 < 210 > 210 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 5 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 6 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 210 tttaynnnnn gtg 13 < 210 > 211 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 11 ) . .( 11 ) < 223 > r is g or a < 400 > 211 taaynnnnnn rttg 14 < 210 > 212 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 212 ccannnnnnt tga 13 < 210 > 213 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is g or a < 400 > 213 ggannnnnnr taa 13 < 210 > 214 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 12 ) . .( 12 ) < 223 > y is t or c < 400 > 214 gannnnnnnt ay 12 < 210 > 215 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is g or a < 220 > < 221 > misc _ feature < 222 > ( 13 ) . .( 13 ) < 223 > y is t or c < 400 > 215 gagnnnnnnr tayg 14 < 210 > 216 < 211 > 15 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 216 gaannnnnnn ntcgc 15 < 210 > 217 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 217 ggtannnnnn tcg 13 < 210 > 218 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 218 gccnnnnnnt cg 12 < 210 > 219 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 400 > 219 ggcannnnnc tc 12 < 210 > 220 < 211 > 15 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 15 ) . .( 15 ) < 223 > y is t or c < 400 > 220 gtannnnnnn ngtcy 15 < 210 > 221 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 221 ggcannnnnn tta 13 < 210 > 222 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > r is g or a < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 222 craannnnnn ctc 13 < 210 > 223 < 211 > 15 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 223 taaynnnnnn ntctt 15 < 210 > 224 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 224 gagnnnnnnn tcc 13 < 210 > 225 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 11 ) < 223 > n is a , c , g , or t < 400 > 225 ccannnnnnn ntgg 14 < 210 > 226 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 8 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is a or g < 400 > 226 gyaynnnngr tg 12 < 210 > 227 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 10 ) < 223 > n is a , c , g , or t < 400 > 227 ttcannnnnn tcc 13 < 210 > 228 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 400 > 228 yacnnnnngt ag 12 < 210 > 229 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is g or a < 400 > 229 tagnnnnnnr tgg 13 < 210 > 230 < 211 > 14 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 3 ) . .( 3 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 10 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 13 ) . .( 13 ) < 223 > y is t or c < 400 > 230 gaynnnnnnn tcyc 14 < 210 > 231 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 8 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 12 ) . .( 12 ) < 223 > y is t or c < 400 > 231 taagnnnnct ay 12 < 210 > 232 < 211 > 12 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 2 ) . .( 2 ) < 223 > y is t or c < 220 > < 221 > misc _ feature < 222 > ( 5 ) . .( 9 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 10 ) . .( 10 ) < 223 > r is g or a < 400 > 232 cytannnnnr tc 12 < 210 > 233 < 211 > 13 < 212 > DNA < 213 > artificial < 220 > < 223 > restriction site < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 8 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 9 ) . .( 9 ) < 223 > r is g or a < 220 > < 221 > misc _ feature < 222 > ( 13 ) . .( 13 ) < 223 > y is t or c < 400 > 233 cccnnnnnrt tgy 13