Then you really don ' t want to hear The Valley Road song <| sep |> In the htslib versions of samtools 1 . 0 the command is now samtools fastq . Proper usage is thissamtools fastq 0 unpaired . fq 1 fwd . fq 2 rev . fq $ bamYou should expect that the 0 file is empty for paired reads , but having it around is a good validation . However , as mentioned elsewhere , sometimes this tool fails to maintain proper pairing . This should not be the case for proper BAMs produced by tools like BWA . If this does happen , you may need to resort by name to fix the problem . <| sep |> Probably during the daily deals that are supposed to start on the 25 th . <| sep |> That blows my mind . An ambulance ride here in America will almost always be more than $ 1 , 000 if you ' re uninsured . To me the anxiety of medical bills is usually worse than the reason for the hospital visit .