Media for Charales 
SWCN–1 
Put bottom mud from a paddy field into a glass vessel up to one-quarter to one-fifth from the bottom. Dampen the mud with deionized water (or distilled water). Cover the glass vessel or jar with a plastic cap or aluminum foil, and autoclave it twice with overnight rest in between (121ºC, 20 min). After cooling the mixture to room temperature, pour sterilized deionized water (or sterilized distilled water) into it carefully (so as not to disturb the soil). 
MICROBIAL CULTURE COLLECTION 
National Institute for Environmental Studies 