Def i ni ng raf t  domai ns i n t he pl asma membrane
Aut horAki hi ro Kusumi ,  Takahi ro K.  Fuj i wara,  Taka A.Tsunoyama,  Ri nshi  S.  Kasai ,  An‐ An Li u,Koi chi ro M.  Hi rosawa,  Masanao Ki noshi t a,Nobuaki  Mat sumori ,  Naoko Komura,  Hi romuneAndo,  Keni chi  G.  N.  Suzuki
j ournal  orpubl i cat i on t i t l e
Traf f i c
vol ume21
number1
page range106- 137
year2019- 12- 19
Publ i sherJ ohn Wi l ey & Sons
Ri ght s( C)  2019 J ohn Wi l ey & Sons A/S.Thi s i s t he peer revi ewed versi on of  t hef ol l owi ng art i cl e:  Kusumi ,  A,  Fuj i wara,  TK,Tsunoyama,  TA,  Kasai ,  RS,  Li u,  AA,  Hi rosawa,KM,  Ki noshi t a,  M,  Mat sumori ,  N,  Komura,  N,Ando,  H,  Suzuki ,  K.  GN,  Def i ni ng raf t  domai nsi n t he pl asma membrane.  Traf f i c.  2020;  21:  106 137. ,  whi ch has been publ i shed i n f i nal  f ormat  ht t ps: //doi . org/10. 1111/t ra. 12718.  Thi sart i cl e may be used f or non- commerci alpurposes i n accordance wi t h Wi l ey Terms andCondi t i ons f or Use of  Sel f - Archi ved Versi ons.
Aut hor' s f l agaut hor
URLht t p: //i d. ni i . ac. j p/1394/00001319/
doi: info:doi/10.1111/tra.12718
BodipyFL-PC594neg-SM
594neg-DOPC488neg-SM
Fig. 1  Kusumi et al.
ATTO488ATTO594
ATTO594
ATTO594
488neg-SM594neg-SM
594neg-DSPC
594neg-DOPC
ATTO594
594-DOPE
ATTO594
Fig. 2  Kusumi et al.
488neg-SM594neg-DOPC
594neg-SMBodipyFl-PC
Fig. 3  Kusumi et al.
(A) Fluorescent GM3 analogs
(B) Fluorescent GM1 analogs
(C) Fluorescent GM2 analogs
(D) Fluorescent GD1b analog
OOAcHN
OC13H27
C17H35
C17H35
C13H27
OHOHHO
C13H27
C17H35
C13H27
OOC13H27
HNC17H35
HOHOOH
R2(S9)
R1(G6)
R2(S9)
R1(termG6)
R2(S9)
R1(GN6)
R(termG6)
Fig. 4  Kusumi et al.
  1 (TMR-G6-GM3) R1 = NHC(=O)-TMR, R2 = OH   2 (594-G6-GM3) R1 = NHC(=O)-ATTO594, R2 = OH   3 (TMR-S9-GM3) R1 = OH, R2 = NHC(=O)-TMR   4 (FI-S9-GM3) R1 = OH, R2 = NHC(=O)-FI   5 (594-S9-GM3) R1 = OH, R2 = NHC(=O)-ATTO594  6 (647N-S9-GM3) R1 = OH, R2 = NHC(=O)-ATTO647N  7 (488-S9-GM3) R1 = OH, R2 = NHC(=O)-ATTO488
  8 (TMR-S9-GM1) R1 = OH, R2 = NHC(=O)-TMR   9 (594-S9-GM1) R1 = OH, R2 = NHC(=O)-ATTO59410 (488-S9-GM1) R1 = OH, R2 = NHC(=O)-ATTO48811 (594-termG6-GM1) R1 = NHC(=O)-ATTO594, R2 = OH 
12 (BodipyFL-C5-GM1) R = BodipyFL-C5 
13 (594-S9-GM2) R1 = OH, R2 = NHC(=O)-ATTO59414 (594-GN6-GM2) R1 = NHC(=O)-ATTO594, R2 = OH 
15 (594-termG6-GD1b) R = NHC(=O)-ATTO594 