Media for freshwater, terrestrial, hot spring and salt water algae 
BG–11 
NaNO3  150 mg 
K2HPO4 · 3H2O  4 mg 
MgSO4 · 7H2O  7.5 mg 
CaCl2 · 2H2O  3.6 mg 
Citric acid  0.6 mg 
Ferric ammonium citrate  0.6 mg 
Na2EDTA - Mg  0.1 mg 
Na2CO3  2 mg 
Trace metal mix A5 + Co  0.1 mL 
Distilled water  99.9 mL 
pH 7.4  
 Add 1.5 g agar to 100 mL of medium to give a solid medium.  Reference Waterbury, J. B., Stanier, R. Y.  1981  Isolation and growth of cyanobacteria from marine and hypersaline environments.  The Prokaryotes, 7, 221-223. 
Trace metal mix A5 + Co 
H3BO3  286 mg 
MnCl2 · 4H2O  181 mg  
ZnSO4 · 7H2O  22.2 mg 
Na2MoO4 · 2H2O1)  3.9 mg 
CuSO4 · 5H2O  7.9 mg 
Co(NO3)2·6H2O  4.9 mg 
Distilled water  100 mL 
 1) In the NIES-Collection, Na2MoO4 · 2H2O is increased from 3.9 mg to 39 mg. 
 Reference Waterbury, J. B., Stanier, R. Y.  1981  Isolation and growth of cyanobacteria from marine and hypersaline environments.  The Prokaryotes, 7, 221-223. 
MICROBIAL CULTURE COLLECTION National Institute for Environmental Studies 