Cross-Talk in the Innate Immune System: Neutrophils Instruct Recruitment and Activation of Dendritic Cells during Microbial Infection
Soumaya Bennouna, Susan K. Bliss, Tyler J. Curiel and Eric Y. Denkers
J Immunol December 1, 2003, 171 (11) 6052-6058; DOI: https://doi.org/10.4049/jimmunol.171.11.6052
Soumaya Bennouna
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853; and
Susan K. Bliss
Tyler J. Curiel
Tulane Medical School, Tulane University, New Orleans, LA 70112
Eric Y. Denkers
Type I inflammatory cytokines are essential for immunity to many microbial pathogens, including Toxoplasma gondii. Dendritic cells (DC) are key to initiating type 1 immunity, but neutrophils are also a source of chemokines and cytokines involved in Th1 response ignition. We found that T. gondii triggered neutrophil synthesis of CC chemokine ligand (CCL)3, CCL4, CCL5, and CCL20, chemokines that were strongly chemotactic for immature DC. Moreover, supernatants obtained from parasite-stimulated polymorphonuclear leukocytes induced DC IL-12(p40) and TNF-α production. Parasite-triggered neutrophils also released factors that induced DC CD40 and CD86 up-regulation, and this response was dependent upon parasite-triggered neutrophil TNF-α production. In vivo evidence that polymorphonuclear leukocytes exert an important influence on DC activation was obtained by examining splenic DC cytokine production following infection of neutrophil-depleted mice. These animals displayed severely curtailed splenic DC IL-12 and TNF-α production, as revealed by ex vivo flow cytometric analysis and in vitro culture assay. Our results reveal a previously unrecognized regulatory role for neutrophils in DC function during microbial infection, and suggest that cross-talk between these cell populations is an important component of the innate immune response to infection.
Received June 6, 2003.
Vol. 171, Issue 11
You are going to email the following Cross-Talk in the Innate Immune System: Neutrophils Instruct Recruitment and Activation of Dendritic Cells during Microbial Infection
Soumaya Bennouna, Susan K. Bliss, Tyler J. Curiel, Eric Y. Denkers
The Journal of Immunology December 1, 2003, 171 (11) 6052-6058; DOI: 10.4049/jimmunol.171.11.6052