Increase in pulmonary vascular permeability caused by increased adhesiveness of polymorphonuclear leukocytes and superoxide
T. Tanita, C. Song, S. Ueda, Y. Hoshikawa, Y. Ashino, M. Noda, T. Tabata, S. Suzuki, S. Ono, S. Fujimura
We report that mechanical stimulation of human neutrophils results in their accumulation in isolated rat lungs and in an increase in pulmonary vascular permeability. To determine whether reactive oxygen species were involved in this increase and, if so, whether it is mediate by xanthine oxidase metabolites, we assessed the effect of stimulated and unstimulated neutrophils, and of a superoxide scavenger, superoxide dismutase (SOD), and a xanthine oxidase inhibitor, allopurinol (ALLO) on pulmonary vascular permeability in isolated perfused lungs from Sprague-Dawley rats. Pulmonary vascular permeability in isolated rat lungs was assessed using a filtration coefficient determined by gravimetry. To quantify neutrophil accumulation in the lung, we measured myeloperoxydase (MPO). Neutrophils were stimulated by gentle agitation in a glass container for 10 s and Mac-1 was subsequently upregulated on the surface of the neutrophils. In lungs that received stimulated neutrophils, the pulmonary vascular filtration coefficient was about 5 times higher than in lungs that received unstimulated neutrophils. An increase in filtration coefficient was almost completely blocked by pretreatment with SOD or ALLO. However, the accumulation of stimulated neutrophils was not, or only partly, blocked by SOD or ALLO, respectively. We conclude that the increase in pulmonary vascular permeability caused by mechanically stimulated neutrophils was partly mediated by reactive oxygen species generated via the xanthine oxidase system.
Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society
Published - 02-1998
Dive into the research topics of 'Increase in pulmonary vascular permeability caused by increased adhesiveness of polymorphonuclear leukocytes and superoxide'. Together they form a unique fingerprint.
Adhesiveness Medicine & Life Sciences 100%
Capillary Permeability Medicine & Life Sciences 82%
Superoxides Medicine & Life Sciences 75%
Neutrophils Medicine & Life Sciences 58%
Allopurinol Medicine & Life Sciences 25%
Xanthine Oxidase Medicine & Life Sciences 24%
Superoxide Dismutase Medicine & Life Sciences 18%
Tanita, T., Song, C., Ueda, S., Hoshikawa, Y., Ashino, Y., Noda, M., Tabata, T., Suzuki, S., Ono, S., & Fujimura, S. (1998). Increase in pulmonary vascular permeability caused by increased adhesiveness of polymorphonuclear leukocytes and superoxide. Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society, 36(2), 144-149.
Tanita, T. ; Song, C. ; Ueda, S. et al. / Increase in pulmonary vascular permeability caused by increased adhesiveness of polymorphonuclear leukocytes and superoxide. In: Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society. 1998 ; Vol. 36, No. 2. pp. 144-149.
@article{0bb2ae33c61c476393f3525fd547bbc6,
title = "Increase in pulmonary vascular permeability caused by increased adhesiveness of polymorphonuclear leukocytes and superoxide",
abstract = "We report that mechanical stimulation of human neutrophils results in their accumulation in isolated rat lungs and in an increase in pulmonary vascular permeability. To determine whether reactive oxygen species were involved in this increase and, if so, whether it is mediate by xanthine oxidase metabolites, we assessed the effect of stimulated and unstimulated neutrophils, and of a superoxide scavenger, superoxide dismutase (SOD), and a xanthine oxidase inhibitor, allopurinol (ALLO) on pulmonary vascular permeability in isolated perfused lungs from Sprague-Dawley rats. Pulmonary vascular permeability in isolated rat lungs was assessed using a filtration coefficient determined by gravimetry. To quantify neutrophil accumulation in the lung, we measured myeloperoxydase (MPO). Neutrophils were stimulated by gentle agitation in a glass container for 10 s and Mac-1 was subsequently upregulated on the surface of the neutrophils. In lungs that received stimulated neutrophils, the pulmonary vascular filtration coefficient was about 5 times higher than in lungs that received unstimulated neutrophils. An increase in filtration coefficient was almost completely blocked by pretreatment with SOD or ALLO. However, the accumulation of stimulated neutrophils was not, or only partly, blocked by SOD or ALLO, respectively. We conclude that the increase in pulmonary vascular permeability caused by mechanically stimulated neutrophils was partly mediated by reactive oxygen species generated via the xanthine oxidase system.",
author = "T. Tanita and C. Song and S. Ueda and Y. Hoshikawa and Y. Ashino and M. Noda and T. Tabata and S. Suzuki and S. Ono and S. Fujimura",
journal = "Respiratory Investigation",
Tanita, T, Song, C, Ueda, S, Hoshikawa, Y, Ashino, Y, Noda, M, Tabata, T, Suzuki, S, Ono, S & Fujimura, S 1998, 'Increase in pulmonary vascular permeability caused by increased adhesiveness of polymorphonuclear leukocytes and superoxide', Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society, vol. 36, no. 2, pp. 144-149.
Increase in pulmonary vascular permeability caused by increased adhesiveness of polymorphonuclear leukocytes and superoxide. / Tanita, T.; Song, C.; Ueda, S. et al.
In: Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society, Vol. 36, No. 2, 02.1998, p. 144-149.
T1 - Increase in pulmonary vascular permeability caused by increased adhesiveness of polymorphonuclear leukocytes and superoxide
AU - Tanita, T.
AU - Song, C.
AU - Ueda, S.
AU - Hoshikawa, Y.
AU - Ashino, Y.
AU - Noda, M.
AU - Tabata, T.
AU - Suzuki, S.
AU - Ono, S.
AU - Fujimura, S.
N2 - We report that mechanical stimulation of human neutrophils results in their accumulation in isolated rat lungs and in an increase in pulmonary vascular permeability. To determine whether reactive oxygen species were involved in this increase and, if so, whether it is mediate by xanthine oxidase metabolites, we assessed the effect of stimulated and unstimulated neutrophils, and of a superoxide scavenger, superoxide dismutase (SOD), and a xanthine oxidase inhibitor, allopurinol (ALLO) on pulmonary vascular permeability in isolated perfused lungs from Sprague-Dawley rats. Pulmonary vascular permeability in isolated rat lungs was assessed using a filtration coefficient determined by gravimetry. To quantify neutrophil accumulation in the lung, we measured myeloperoxydase (MPO). Neutrophils were stimulated by gentle agitation in a glass container for 10 s and Mac-1 was subsequently upregulated on the surface of the neutrophils. In lungs that received stimulated neutrophils, the pulmonary vascular filtration coefficient was about 5 times higher than in lungs that received unstimulated neutrophils. An increase in filtration coefficient was almost completely blocked by pretreatment with SOD or ALLO. However, the accumulation of stimulated neutrophils was not, or only partly, blocked by SOD or ALLO, respectively. We conclude that the increase in pulmonary vascular permeability caused by mechanically stimulated neutrophils was partly mediated by reactive oxygen species generated via the xanthine oxidase system.
AB - We report that mechanical stimulation of human neutrophils results in their accumulation in isolated rat lungs and in an increase in pulmonary vascular permeability. To determine whether reactive oxygen species were involved in this increase and, if so, whether it is mediate by xanthine oxidase metabolites, we assessed the effect of stimulated and unstimulated neutrophils, and of a superoxide scavenger, superoxide dismutase (SOD), and a xanthine oxidase inhibitor, allopurinol (ALLO) on pulmonary vascular permeability in isolated perfused lungs from Sprague-Dawley rats. Pulmonary vascular permeability in isolated rat lungs was assessed using a filtration coefficient determined by gravimetry. To quantify neutrophil accumulation in the lung, we measured myeloperoxydase (MPO). Neutrophils were stimulated by gentle agitation in a glass container for 10 s and Mac-1 was subsequently upregulated on the surface of the neutrophils. In lungs that received stimulated neutrophils, the pulmonary vascular filtration coefficient was about 5 times higher than in lungs that received unstimulated neutrophils. An increase in filtration coefficient was almost completely blocked by pretreatment with SOD or ALLO. However, the accumulation of stimulated neutrophils was not, or only partly, blocked by SOD or ALLO, respectively. We conclude that the increase in pulmonary vascular permeability caused by mechanically stimulated neutrophils was partly mediated by reactive oxygen species generated via the xanthine oxidase system.
JO - Respiratory Investigation
JF - Respiratory Investigation
Tanita T, Song C, Ueda S, Hoshikawa Y, Ashino Y, Noda M et al. Increase in pulmonary vascular permeability caused by increased adhesiveness of polymorphonuclear leukocytes and superoxide. Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society. 1998 Feb;36(2):144-149.