BER Structural Biology and Imaging Resources
Synchrotron, Neutron, and Cryo-EM
U.S. Department of Energy | Office of Science | Office of Biological and Environmental Research
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Techniques Overview
Cryogenic Electron Microscopy and Tomography
Neutron Macromolecular Crystallography
Small-Angle Neutron Scattering
Solution X-ray Scattering (SAXS)
X-Ray Macromolecular Crystallography
Cell and Tissue Structure
Neutron Imaging
Soft X-ray Tomography
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Synchrotron Infrared Hyperspectral Imaging
X-Ray Absorption and Emission Spectroscopy
X-Ray Fluorescence Imaging
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Advanced Light Source
Environmental Molecular Sciences Laboratory
National Synchrotron Light Source II
Spallation Neutron Source and High Flux Isotope Reactor
Stanford Synchrotron Radiation Lightsource
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First X-ray Nanofluorescence Tomography of Single Bacterial Cell
The elemental distribution and nanostructure of single E. coli cell were co-localized simultaneously using X-ray fluorescence (XRF) nanotomography and ptychography using a sub-15 nm beam. This multimodal approach presents new possibilities in understanding subcellular biochemistry in individual organelles, which are usually analyzed at the population level.
T. W. Victor, L. M. Easthon, M. Ge, K. H. O'Toole, R. J. Smith, X. Huang, H. Yan, K. N. Allen, Y. S. Chu, L. M. Miller. "X-ray Fluorescence Nanotomography of Single Bacteria with a Sub-15 nm Beam." Scientific Reports. 8: 13415 (2018). [DOI: 10.1038/s41598-018-31461-y]
Structural Biology and Imaging Resources are a collection of enabling capabilities sponsored by the U.S. Department of Energy's (DOE) Office of Biological and Environmental Research. These x-ray, neutron, and electron resources and beamlines are located at scientific user facilities supported by DOE's Office of Basic Energy Sciences.