Value of the ethnomedical information for the discovery of plants with antifungal properties. A survey among seven Latin American countries
Laura Svetaz, Federico Zuljan, Marcos Derita, Elisa Petenatti, Giselle Tamayo, Armando Cáceres, Valdir Cechinel Filho, Alberto José Gimenez Turba, Roberto Pinzón, Susana A. Zacchino, Mahabir Gupta
Facultad de Ciencias Farmacéuticas y Bioquímicas
Instituto de Investigaciones Fármaco Bioquímicas
Ethnopharmacological relevance: This study reports the antifungal evaluation of 327 plant species (92 families and 251 genera) from seven Latin American countries which were selected on the basis of their reported ethnomedical uses and compared them with plants selected at random. Aim of the study: (a) The main aim of this study was to investigate whether the probability of detecting antifungal plants is higher when plants have reports of ethnopharmacological uses related to fungal infections (PAU group) than when they are selected at random (PNAU group). (b) The second objective was to determine, within the PAU group, whether the probability of obtaining a positive result will be higher when the plants are tested against dermatophytes, than against yeasts or Aspergillus spp. (c) The third goal was to investigate, within all MICs ≤ 1000 μg/mL, if the MICs displayed by the PAU group are comparatively lower than MIC values of the PNAU group; that is to say, if they can be expected more potent antifungal plants within the group of plants that have a history of traditional use related to fungal infections than when they do not have one. Materials and methods: A five-stage process of documentation, evaluation and analysis of results was conducted: (1) selection of words that could describe the ethnopharmacological use related to fungal infections; (2) a survey of specialized literature in each country; (3) collection and preparation of an extract of each plant; (4) antifungal evaluation of the selected plants and (5) statistical analysis of the results. For the antifungal evaluation, the microbroth dilution assay recommended by the Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS) was used against a panel of eleven human opportunistic and pathogenic fungi. For the statistical analysis the Pearson's Chi Square test and the Score's test were used. Results: (a) A significantly higher probability of detecting plants with antifungal activity against at least one fungus was found within the PAU (40.3%) than the PNAU group (21.3%) (p < 0.01). (b) A similar higher probability than in (a) (39.6% vs. 20.8%) was found when plants were tested against dermatophytes (p < 0.01) but not against yeasts or Aspergillus spp. (p > 0.05). (c) Within the detected antifungal plants from both groups, plants of the PAU group displayed higher activities (lower MICs) than those of PNAU group against dermatophytes (p < 0.05) but not against yeasts or Aspergillus spp. Conclusions: Considering that dermatophytes are the cause of superficial fungal infections, which can be easily detected and followed by traditional healers, our findings suggest that the ethnopharmacological approach is useful in guiding the detection of antifungal plants in Latin America mainly for infections in which the pathological expression is obvious and, therefore, the cure can be clearly observed.
https://doi.org/10.1016/j.jep.2009.09.034
Authors acknowledge support of the Organization of American States (OAS) for a grant to CIFLORPAN; National Agency for Science and Technology of Argentina (ANPCyT); Iberoamerican Program of Science and Technology for Development (CYTED-RIBIOFAR) and Conselho Nacional de Pesquisas e Desenvolvimento Tecnologico of Brasil (CNPq). LS acknowledges to ANPCyT, MGD thanks to Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and FZ thanks to Fundación del Banco de Santa Fe, for respective fellowships.
10.1016/j.jep.2009.09.034
Profundice en los temas de investigación de 'Value of the ethnomedical information for the discovery of plants with antifungal properties. A survey among seven Latin American countries'. En conjunto forman una huella única.
Antifungal Química 100%
Dilution Química 82%
Mycoses Medicina y ciencias biológicas 34%
Arthrodermataceae Medicina y ciencias biológicas 33%
Aspergillus Medicina y ciencias biológicas 19%
Fungi Medicina y ciencias biológicas 15%
Yeasts Medicina y ciencias biológicas 13%
Latin America Medicina y ciencias biológicas 10%
Svetaz, L., Zuljan, F., Derita, M., Petenatti, E., Tamayo, G., Cáceres, A., Cechinel Filho, V., Gimenez Turba, A. J., Pinzón, R., Zacchino, S. A., & Gupta, M. (2010). Value of the ethnomedical information for the discovery of plants with antifungal properties. A survey among seven Latin American countries. Journal of Ethnopharmacology, 127(1), 137-158. https://doi.org/10.1016/j.jep.2009.09.034
Svetaz, Laura ; Zuljan, Federico ; Derita, Marcos et al. / Value of the ethnomedical information for the discovery of plants with antifungal properties. A survey among seven Latin American countries. En: Journal of Ethnopharmacology. 2010 ; Vol. 127, N.º 1. pp. 137-158.
@article{e50fdcca96a2452ea5afdb2512c144f5,
title = "Value of the ethnomedical information for the discovery of plants with antifungal properties. A survey among seven Latin American countries",
abstract = "Ethnopharmacological relevance: This study reports the antifungal evaluation of 327 plant species (92 families and 251 genera) from seven Latin American countries which were selected on the basis of their reported ethnomedical uses and compared them with plants selected at random. Aim of the study: (a) The main aim of this study was to investigate whether the probability of detecting antifungal plants is higher when plants have reports of ethnopharmacological uses related to fungal infections (PAU group) than when they are selected at random (PNAU group). (b) The second objective was to determine, within the PAU group, whether the probability of obtaining a positive result will be higher when the plants are tested against dermatophytes, than against yeasts or Aspergillus spp. (c) The third goal was to investigate, within all MICs ≤ 1000 μg/mL, if the MICs displayed by the PAU group are comparatively lower than MIC values of the PNAU group; that is to say, if they can be expected more potent antifungal plants within the group of plants that have a history of traditional use related to fungal infections than when they do not have one. Materials and methods: A five-stage process of documentation, evaluation and analysis of results was conducted: (1) selection of words that could describe the ethnopharmacological use related to fungal infections; (2) a survey of specialized literature in each country; (3) collection and preparation of an extract of each plant; (4) antifungal evaluation of the selected plants and (5) statistical analysis of the results. For the antifungal evaluation, the microbroth dilution assay recommended by the Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS) was used against a panel of eleven human opportunistic and pathogenic fungi. For the statistical analysis the Pearson's Chi Square test and the Score's test were used. Results: (a) A significantly higher probability of detecting plants with antifungal activity against at least one fungus was found within the PAU (40.3%) than the PNAU group (21.3%) (p < 0.01). (b) A similar higher probability than in (a) (39.6% vs. 20.8%) was found when plants were tested against dermatophytes (p < 0.01) but not against yeasts or Aspergillus spp. (p > 0.05). (c) Within the detected antifungal plants from both groups, plants of the PAU group displayed higher activities (lower MICs) than those of PNAU group against dermatophytes (p < 0.05) but not against yeasts or Aspergillus spp. Conclusions: Considering that dermatophytes are the cause of superficial fungal infections, which can be easily detected and followed by traditional healers, our findings suggest that the ethnopharmacological approach is useful in guiding the detection of antifungal plants in Latin America mainly for infections in which the pathological expression is obvious and, therefore, the cure can be clearly observed.",
keywords = "Antifungal activity, Ethnomedical information, Latin American plants",
author = "Laura Svetaz and Federico Zuljan and Marcos Derita and Elisa Petenatti and Giselle Tamayo and Armando C{\'a}ceres and {Cechinel Filho}, Valdir and {Gimenez Turba}, {Alberto Jos{\'e}} and Roberto Pinz{\'o}n and Zacchino, {Susana A.} and Mahabir Gupta",
note = "Funding Information: Authors acknowledge support of the Organization of American States (OAS) for a grant to CIFLORPAN; National Agency for Science and Technology of Argentina (ANPCyT); Iberoamerican Program of Science and Technology for Development (CYTED-RIBIOFAR) and Conselho Nacional de Pesquisas e Desenvolvimento Tecnologico of Brasil (CNPq). LS acknowledges to ANPCyT, MGD thanks to Consejo Nacional de Investigaciones Cient{\'i}ficas y T{\'e}cnicas (CONICET) and FZ thanks to Fundaci{\'o}n del Banco de Santa Fe, for respective fellowships.",
doi = "10.1016/j.jep.2009.09.034",
Svetaz, L, Zuljan, F, Derita, M, Petenatti, E, Tamayo, G, Cáceres, A, Cechinel Filho, V, Gimenez Turba, AJ, Pinzón, R, Zacchino, SA & Gupta, M 2010, 'Value of the ethnomedical information for the discovery of plants with antifungal properties. A survey among seven Latin American countries', Journal of Ethnopharmacology, vol. 127, n.º 1, pp. 137-158. https://doi.org/10.1016/j.jep.2009.09.034
Value of the ethnomedical information for the discovery of plants with antifungal properties. A survey among seven Latin American countries. / Svetaz, Laura; Zuljan, Federico; Derita, Marcos et al.
En: Journal of Ethnopharmacology, Vol. 127, N.º 1, 08.01.2010, p. 137-158.
T1 - Value of the ethnomedical information for the discovery of plants with antifungal properties. A survey among seven Latin American countries
AU - Svetaz, Laura
AU - Zuljan, Federico
AU - Derita, Marcos
AU - Petenatti, Elisa
AU - Tamayo, Giselle
AU - Cáceres, Armando
AU - Cechinel Filho, Valdir
AU - Gimenez Turba, Alberto José
AU - Pinzón, Roberto
AU - Zacchino, Susana A.
AU - Gupta, Mahabir
N1 - Funding Information: Authors acknowledge support of the Organization of American States (OAS) for a grant to CIFLORPAN; National Agency for Science and Technology of Argentina (ANPCyT); Iberoamerican Program of Science and Technology for Development (CYTED-RIBIOFAR) and Conselho Nacional de Pesquisas e Desenvolvimento Tecnologico of Brasil (CNPq). LS acknowledges to ANPCyT, MGD thanks to Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and FZ thanks to Fundación del Banco de Santa Fe, for respective fellowships.
N2 - Ethnopharmacological relevance: This study reports the antifungal evaluation of 327 plant species (92 families and 251 genera) from seven Latin American countries which were selected on the basis of their reported ethnomedical uses and compared them with plants selected at random. Aim of the study: (a) The main aim of this study was to investigate whether the probability of detecting antifungal plants is higher when plants have reports of ethnopharmacological uses related to fungal infections (PAU group) than when they are selected at random (PNAU group). (b) The second objective was to determine, within the PAU group, whether the probability of obtaining a positive result will be higher when the plants are tested against dermatophytes, than against yeasts or Aspergillus spp. (c) The third goal was to investigate, within all MICs ≤ 1000 μg/mL, if the MICs displayed by the PAU group are comparatively lower than MIC values of the PNAU group; that is to say, if they can be expected more potent antifungal plants within the group of plants that have a history of traditional use related to fungal infections than when they do not have one. Materials and methods: A five-stage process of documentation, evaluation and analysis of results was conducted: (1) selection of words that could describe the ethnopharmacological use related to fungal infections; (2) a survey of specialized literature in each country; (3) collection and preparation of an extract of each plant; (4) antifungal evaluation of the selected plants and (5) statistical analysis of the results. For the antifungal evaluation, the microbroth dilution assay recommended by the Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS) was used against a panel of eleven human opportunistic and pathogenic fungi. For the statistical analysis the Pearson's Chi Square test and the Score's test were used. Results: (a) A significantly higher probability of detecting plants with antifungal activity against at least one fungus was found within the PAU (40.3%) than the PNAU group (21.3%) (p < 0.01). (b) A similar higher probability than in (a) (39.6% vs. 20.8%) was found when plants were tested against dermatophytes (p < 0.01) but not against yeasts or Aspergillus spp. (p > 0.05). (c) Within the detected antifungal plants from both groups, plants of the PAU group displayed higher activities (lower MICs) than those of PNAU group against dermatophytes (p < 0.05) but not against yeasts or Aspergillus spp. Conclusions: Considering that dermatophytes are the cause of superficial fungal infections, which can be easily detected and followed by traditional healers, our findings suggest that the ethnopharmacological approach is useful in guiding the detection of antifungal plants in Latin America mainly for infections in which the pathological expression is obvious and, therefore, the cure can be clearly observed.
AB - Ethnopharmacological relevance: This study reports the antifungal evaluation of 327 plant species (92 families and 251 genera) from seven Latin American countries which were selected on the basis of their reported ethnomedical uses and compared them with plants selected at random. Aim of the study: (a) The main aim of this study was to investigate whether the probability of detecting antifungal plants is higher when plants have reports of ethnopharmacological uses related to fungal infections (PAU group) than when they are selected at random (PNAU group). (b) The second objective was to determine, within the PAU group, whether the probability of obtaining a positive result will be higher when the plants are tested against dermatophytes, than against yeasts or Aspergillus spp. (c) The third goal was to investigate, within all MICs ≤ 1000 μg/mL, if the MICs displayed by the PAU group are comparatively lower than MIC values of the PNAU group; that is to say, if they can be expected more potent antifungal plants within the group of plants that have a history of traditional use related to fungal infections than when they do not have one. Materials and methods: A five-stage process of documentation, evaluation and analysis of results was conducted: (1) selection of words that could describe the ethnopharmacological use related to fungal infections; (2) a survey of specialized literature in each country; (3) collection and preparation of an extract of each plant; (4) antifungal evaluation of the selected plants and (5) statistical analysis of the results. For the antifungal evaluation, the microbroth dilution assay recommended by the Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS) was used against a panel of eleven human opportunistic and pathogenic fungi. For the statistical analysis the Pearson's Chi Square test and the Score's test were used. Results: (a) A significantly higher probability of detecting plants with antifungal activity against at least one fungus was found within the PAU (40.3%) than the PNAU group (21.3%) (p < 0.01). (b) A similar higher probability than in (a) (39.6% vs. 20.8%) was found when plants were tested against dermatophytes (p < 0.01) but not against yeasts or Aspergillus spp. (p > 0.05). (c) Within the detected antifungal plants from both groups, plants of the PAU group displayed higher activities (lower MICs) than those of PNAU group against dermatophytes (p < 0.05) but not against yeasts or Aspergillus spp. Conclusions: Considering that dermatophytes are the cause of superficial fungal infections, which can be easily detected and followed by traditional healers, our findings suggest that the ethnopharmacological approach is useful in guiding the detection of antifungal plants in Latin America mainly for infections in which the pathological expression is obvious and, therefore, the cure can be clearly observed.
KW - Antifungal activity
KW - Ethnomedical information
KW - Latin American plants
U2 - 10.1016/j.jep.2009.09.034
DO - 10.1016/j.jep.2009.09.034
Svetaz L, Zuljan F, Derita M, Petenatti E, Tamayo G, Cáceres A et al. Value of the ethnomedical information for the discovery of plants with antifungal properties. A survey among seven Latin American countries. Journal of Ethnopharmacology. 2010 ene. 8;127(1):137-158. doi: 10.1016/j.jep.2009.09.034