Use of a highly sensitive assay to analyze the excision repair of dimer and nondimer DNA damages induced in human skin fibroblasts by 254-nm and solar ultraviolet radiation
B. S. Rosenstein, J. T. Murphy, Jonathan M Ducore
The excision repair of nondimer DNA damages induced in normal human skin fibroblasts exposed to the Mylar-filtered UV produced by a fluorescent sunlamp was investigated. This work was accomplished through the development of a modification of the bromodeoxyuridine photolysis assay that greatly increases the sensitivity of this assay. This enhancement in sensitivity was achieved through use of alkaline elution to measure the DNA strand breakage produced by the photolysis of bromodeoxyuridine incorporated into the DNA through excision repair. Using this modified bromodeoxyuridine photolysis, it was found that the solar UV-induced nondimer DNA damages appear to have been repaired by a short patch repair mechanism in which a small number of nucleotides (two to four) were inserted into the repaired site. This is in contrast to the long patch repair process involved in the excision of cyclobutane pyrimidine dimers in which approximately 40 nucleotides were inserted into each repaired region.
Fingerprint Dive into the research topics of 'Use of a highly sensitive assay to analyze the excision repair of dimer and nondimer DNA damages induced in human skin fibroblasts by 254-nm and solar ultraviolet radiation'. Together they form a unique fingerprint.
Photolysis Medicine & Life Sciences
Bromodeoxyuridine Medicine & Life Sciences
Ultraviolet Rays Medicine & Life Sciences
DNA Repair Medicine & Life Sciences
DNA Damage Medicine & Life Sciences
Fibroblasts Medicine & Life Sciences
Nucleotides Medicine & Life Sciences
Rosenstein, B. S., Murphy, J. T., & Ducore, J. M. (1985). Use of a highly sensitive assay to analyze the excision repair of dimer and nondimer DNA damages induced in human skin fibroblasts by 254-nm and solar ultraviolet radiation. Cancer Research, 45(11 I), 5526-5531.
Use of a highly sensitive assay to analyze the excision repair of dimer and nondimer DNA damages induced in human skin fibroblasts by 254-nm and solar ultraviolet radiation. / Rosenstein, B. S.; Murphy, J. T.; Ducore, Jonathan M.
In: Cancer Research, Vol. 45, No. 11 I, 1985, p. 5526-5531.
Rosenstein, BS, Murphy, JT & Ducore, JM 1985, 'Use of a highly sensitive assay to analyze the excision repair of dimer and nondimer DNA damages induced in human skin fibroblasts by 254-nm and solar ultraviolet radiation', Cancer Research, vol. 45, no. 11 I, pp. 5526-5531.
Rosenstein BS, Murphy JT, Ducore JM. Use of a highly sensitive assay to analyze the excision repair of dimer and nondimer DNA damages induced in human skin fibroblasts by 254-nm and solar ultraviolet radiation. Cancer Research. 1985;45(11 I):5526-5531.
Rosenstein, B. S. ; Murphy, J. T. ; Ducore, Jonathan M. / Use of a highly sensitive assay to analyze the excision repair of dimer and nondimer DNA damages induced in human skin fibroblasts by 254-nm and solar ultraviolet radiation. In: Cancer Research. 1985 ; Vol. 45, No. 11 I. pp. 5526-5531.
@article{ffbc99aa744f498aaa342538a662d71c,
title = "Use of a highly sensitive assay to analyze the excision repair of dimer and nondimer DNA damages induced in human skin fibroblasts by 254-nm and solar ultraviolet radiation",
abstract = "The excision repair of nondimer DNA damages induced in normal human skin fibroblasts exposed to the Mylar-filtered UV produced by a fluorescent sunlamp was investigated. This work was accomplished through the development of a modification of the bromodeoxyuridine photolysis assay that greatly increases the sensitivity of this assay. This enhancement in sensitivity was achieved through use of alkaline elution to measure the DNA strand breakage produced by the photolysis of bromodeoxyuridine incorporated into the DNA through excision repair. Using this modified bromodeoxyuridine photolysis, it was found that the solar UV-induced nondimer DNA damages appear to have been repaired by a short patch repair mechanism in which a small number of nucleotides (two to four) were inserted into the repaired site. This is in contrast to the long patch repair process involved in the excision of cyclobutane pyrimidine dimers in which approximately 40 nucleotides were inserted into each repaired region.",
author = "Rosenstein, {B. S.} and Murphy, {J. T.} and Ducore, {Jonathan M}",
journal = "Journal of Cancer Research",
publisher = "American Association for Cancer Research Inc.",
number = "11 I",
T1 - Use of a highly sensitive assay to analyze the excision repair of dimer and nondimer DNA damages induced in human skin fibroblasts by 254-nm and solar ultraviolet radiation
AU - Rosenstein, B. S.
AU - Murphy, J. T.
AU - Ducore, Jonathan M
N2 - The excision repair of nondimer DNA damages induced in normal human skin fibroblasts exposed to the Mylar-filtered UV produced by a fluorescent sunlamp was investigated. This work was accomplished through the development of a modification of the bromodeoxyuridine photolysis assay that greatly increases the sensitivity of this assay. This enhancement in sensitivity was achieved through use of alkaline elution to measure the DNA strand breakage produced by the photolysis of bromodeoxyuridine incorporated into the DNA through excision repair. Using this modified bromodeoxyuridine photolysis, it was found that the solar UV-induced nondimer DNA damages appear to have been repaired by a short patch repair mechanism in which a small number of nucleotides (two to four) were inserted into the repaired site. This is in contrast to the long patch repair process involved in the excision of cyclobutane pyrimidine dimers in which approximately 40 nucleotides were inserted into each repaired region.
AB - The excision repair of nondimer DNA damages induced in normal human skin fibroblasts exposed to the Mylar-filtered UV produced by a fluorescent sunlamp was investigated. This work was accomplished through the development of a modification of the bromodeoxyuridine photolysis assay that greatly increases the sensitivity of this assay. This enhancement in sensitivity was achieved through use of alkaline elution to measure the DNA strand breakage produced by the photolysis of bromodeoxyuridine incorporated into the DNA through excision repair. Using this modified bromodeoxyuridine photolysis, it was found that the solar UV-induced nondimer DNA damages appear to have been repaired by a short patch repair mechanism in which a small number of nucleotides (two to four) were inserted into the repaired site. This is in contrast to the long patch repair process involved in the excision of cyclobutane pyrimidine dimers in which approximately 40 nucleotides were inserted into each repaired region.
JO - Journal of Cancer Research
JF - Journal of Cancer Research
IS - 11 I