Extrachromosomal circular copies of the transposon Tc1
Anthony D. Radice, Scott W. Emmons
The 1.6 kb Tc1 transposable element of Caenorhabditis elegans undergoes excision and transposition in the germline. In somatic tissue it is excised at high frequency. Extrachromosomal linear and circular copies of Tc1 have been identified that are likely to be products of somatic and germline excision. In the present study, we have determined the sequences of the sites of circularization in circular extrachromosomal Tc1 molecules. DNA molecules containing these sites were cloned after PCR amplification with primers directed outward from within Tc1. Sequences were obtained with two complete Tc1 ends and one or more intervening copies of the TA dinucleotide, with one complete end and one deleted end, and with two deleted ends. The 24 clones had different structures, indicating the pool of molecules serving as PCR templates was heterogeneous. The predominant circular Junction had one or more nucleotides deleted from at least one transposon end. Such a molecule without two complete ends might not be expected to serve as a transposition intermediate. Hence, some extrachromosomal circular Tc1 molecules may result from a deadend excision pathway.
https://doi.org/10.1093/nar/21.11.2663
Published - Jun 11 1993
10.1093/nar/21.11.2663
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DNA Transposable Elements Medicine & Life Sciences
Polymerase Chain Reaction Medicine & Life Sciences
Caenorhabditis elegans Medicine & Life Sciences
Nucleotides Medicine & Life Sciences
Radice, A. D., & Emmons, S. W. (1993). Extrachromosomal circular copies of the transposon Tc1. Nucleic acids research, 21(11), 2663-2667. https://doi.org/10.1093/nar/21.11.2663
Extrachromosomal circular copies of the transposon Tc1. / Radice, Anthony D.; Emmons, Scott W.
In: Nucleic acids research, Vol. 21, No. 11, 11.06.1993, p. 2663-2667.
Radice, AD & Emmons, SW 1993, 'Extrachromosomal circular copies of the transposon Tc1', Nucleic acids research, vol. 21, no. 11, pp. 2663-2667. https://doi.org/10.1093/nar/21.11.2663
Radice AD, Emmons SW. Extrachromosomal circular copies of the transposon Tc1. Nucleic acids research. 1993 Jun 11;21(11):2663-2667. https://doi.org/10.1093/nar/21.11.2663
Radice, Anthony D. ; Emmons, Scott W. / Extrachromosomal circular copies of the transposon Tc1. In: Nucleic acids research. 1993 ; Vol. 21, No. 11. pp. 2663-2667.
@article{b6ab0d64abf34580be20c5e8c93d7fb1,
title = "Extrachromosomal circular copies of the transposon Tc1",
abstract = "The 1.6 kb Tc1 transposable element of Caenorhabditis elegans undergoes excision and transposition in the germline. In somatic tissue it is excised at high frequency. Extrachromosomal linear and circular copies of Tc1 have been identified that are likely to be products of somatic and germline excision. In the present study, we have determined the sequences of the sites of circularization in circular extrachromosomal Tc1 molecules. DNA molecules containing these sites were cloned after PCR amplification with primers directed outward from within Tc1. Sequences were obtained with two complete Tc1 ends and one or more intervening copies of the TA dinucleotide, with one complete end and one deleted end, and with two deleted ends. The 24 clones had different structures, indicating the pool of molecules serving as PCR templates was heterogeneous. The predominant circular Junction had one or more nucleotides deleted from at least one transposon end. Such a molecule without two complete ends might not be expected to serve as a transposition intermediate. Hence, some extrachromosomal circular Tc1 molecules may result from a deadend excision pathway.",
author = "Radice, {Anthony D.} and Emmons, {Scott W.}",
note = "Funding Information: We are grateful to the members of the Emmons lab for their advice and comments on the manuscript. We thank Dr Colin Campbell for numerous discussions and advise. This work was supported by NIH grant GM38174. ADR was supported by Training Grant T32CA09060. SWE was the recipient of the Harry Winston Established Fellowship for Research of the American Heart Association.",
doi = "10.1093/nar/21.11.2663",
journal = "Nucleic Acids Research",
T1 - Extrachromosomal circular copies of the transposon Tc1
AU - Radice, Anthony D.
AU - Emmons, Scott W.
N1 - Funding Information: We are grateful to the members of the Emmons lab for their advice and comments on the manuscript. We thank Dr Colin Campbell for numerous discussions and advise. This work was supported by NIH grant GM38174. ADR was supported by Training Grant T32CA09060. SWE was the recipient of the Harry Winston Established Fellowship for Research of the American Heart Association.
N2 - The 1.6 kb Tc1 transposable element of Caenorhabditis elegans undergoes excision and transposition in the germline. In somatic tissue it is excised at high frequency. Extrachromosomal linear and circular copies of Tc1 have been identified that are likely to be products of somatic and germline excision. In the present study, we have determined the sequences of the sites of circularization in circular extrachromosomal Tc1 molecules. DNA molecules containing these sites were cloned after PCR amplification with primers directed outward from within Tc1. Sequences were obtained with two complete Tc1 ends and one or more intervening copies of the TA dinucleotide, with one complete end and one deleted end, and with two deleted ends. The 24 clones had different structures, indicating the pool of molecules serving as PCR templates was heterogeneous. The predominant circular Junction had one or more nucleotides deleted from at least one transposon end. Such a molecule without two complete ends might not be expected to serve as a transposition intermediate. Hence, some extrachromosomal circular Tc1 molecules may result from a deadend excision pathway.
AB - The 1.6 kb Tc1 transposable element of Caenorhabditis elegans undergoes excision and transposition in the germline. In somatic tissue it is excised at high frequency. Extrachromosomal linear and circular copies of Tc1 have been identified that are likely to be products of somatic and germline excision. In the present study, we have determined the sequences of the sites of circularization in circular extrachromosomal Tc1 molecules. DNA molecules containing these sites were cloned after PCR amplification with primers directed outward from within Tc1. Sequences were obtained with two complete Tc1 ends and one or more intervening copies of the TA dinucleotide, with one complete end and one deleted end, and with two deleted ends. The 24 clones had different structures, indicating the pool of molecules serving as PCR templates was heterogeneous. The predominant circular Junction had one or more nucleotides deleted from at least one transposon end. Such a molecule without two complete ends might not be expected to serve as a transposition intermediate. Hence, some extrachromosomal circular Tc1 molecules may result from a deadend excision pathway.
U2 - 10.1093/nar/21.11.2663
DO - 10.1093/nar/21.11.2663
JO - Nucleic Acids Research
JF - Nucleic Acids Research