Effect of salt stress on photosystem Ⅱ heterogeneity in wheat leaves
The regulation and catalytic mechanism of the NADP-malic enzyme from tobacco leaves
Disease Development Caused by Ascochyta rabiei on Chickpea Detached-Leaves in Petri Dishes
Foliar virulence of isolates of Phytophthora infestans sensu lato on detached leaves of two Solanum betaceum cultivars
Extraction of Flavonoids and Quantification of Rutin from waste Tobacco Leaves
Analysis of cellulose content in stalks and leaves of large leaf tobacco
Radioactivity of Tobacco Leaves and Radiation Dose Induced from Smoking
SYNTHESIS OF UBIQUINONE (Co-Q10) FROM NATURAL TOBACCO LEAVES
Effects of Different Rates of Indole-3-Acitic Acid on Root Formation of Detached Leaves of Cowpea (Vigna unguiculata L. Walp)
Proteomic characterisation of endoplasmic reticulum-derived protein bodies in tobacco leaves
American Journal of Plant Sciences 2015
Different Effects of Malate on the Activities of Photosystem II in Detached Leaves of Maize and Tobacco
DOI: 10.4236/ajps.2015.611173, PP. 1734-1741
Zhenhai Cui, Ao Zhang, Ziling Hu, Lijun Zhang, Jinjuan Fan, Yanshu Zhu, Kai Hu, Yanye Ruan, Yixin Guan
Keywords: Malate, Photosystem II, Chlorophyll Fluorescence, Maize, Tobacco
Malate is the first stable product after CO2 is fixed in NADP-dependent malic enzyme (NADP-ME) type of C4 plants, which transfers CO2 and the reducing equivalent from mesophyll cell (MC) to vascular bundle sheath cell (BSC) chloroplasts and affects the redox state of BSC. The aim of this experiment is to investigate the effect of exogenous malate on the activity of photosystem II (PS II) in C4 and C3 plants. The leaf discs from the 5th fully expanded leaves of maize (NADP-ME type C4 plants) and the 10th fully expanded leaves of tobacco (C3 plants) were treated with malate of 50, 100 μM and the chlorophyll fluorescence parameters were measured. Malate treatments decreased the photochemical reaction efficiency (FV/FM) in maize leaves, as a result of rising in initial fluorescence (FO) and decreasing in maximal fluorescence (FM). The number of active PS II reaction center (RC) per excited cross section (RC/CS) declined in malate-treated maize, suggesting that malate inactivated PS II RC. Malate treatments also increased Wk, representing the severity of oxygen-evolving complex (OEC) damage, and decreased the rate of photosynthetic oxygen evolution. We conclude that exogenous malate regulates the activity and structure of PS II in C4 plant maize. No significant changes in the activity of PS II were observed in malate-treated C3 plant tobacco. It is suggested that the short term malate treatment will inhibit PS II of leaves which have C4 anatomy and C4 enzymes.
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