Dectin-1/CLEC7A
Dectin-1/CLEC7A Antibodies
Mouse Dectin-1/CLEC7A Antibody (AF1756)
Mouse Dectin-1/CLEC7A Antibody
Catalog # AF1756
AF1756-SP
Species Reactivity: Mouse
Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Dectin-1/CLEC7A
Phe69-Leu244
Accession # Q6QLQ4
Applications: Block/Neutralize, CyTOF-ready, Flow Cytometry, Western Blot
Label: Unconjugated
Detects mouse Dectin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human Dectin-1 and recombinant mouse Dectin-2 alpha is observed.
View Full Technical Details for Mouse Dectin-1/CLEC7A Antibody
Applications for Mouse Dectin-1/CLEC7A Antibody
Western Blot 0.1 µg/mL Recombinant Mouse Dectin‑1/CLEC7A (Catalog # 1756-DC)
Flow Cytometry 2.5 µg/106 cells RAW 264.7 mouse monocyte/macrophage cell line
Blockade of Receptor-ligand Interaction In a functional ELISA, 0.1-0.4 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Mouse Dectin-1/CLEC7A (Catalog # 1756-DC) coated at 250 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Endotoxin level <0.10 EU per 1 μg of the antibody by the LAL method.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Background: Dectin-1/CLEC7A
Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin-1, respectively. It shares 25-34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8-10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11-13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).
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Entrez Gene IDs 64581 (Human); 56644 (Mouse)
Alternate Names BGR, Beta-glucan receptor, C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12, C-type lectin domain family 7 member A, C-type lectin domain family 7, member A, C-type lectin superfamily member 12, CD369, CLEC7A, CLECSF12, CLECSF12DC-associated C-type lectin 1, DECTIN1CANDF4, Dectin-1, Dectin1, Dendritic cell-associated C-type lectin 1, dendritic cell-associated C-type lectin-1, hDectin-1, lectin-like receptor 1
Citations for Mouse Dectin-1/CLEC7A Antibody (4)
All Applications ICC Neutralization Western Blot
All Sample Types Cell Lysates Whole Cells
Citations are publications that use Bio-Techne products. Selected citations for Mouse Dectin-1/CLEC7A Antibody include:
JK Götzl et al. (2019), Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism EMBO Mol Med, 0(0).
Yamasaki T et al. (2014), The dectin 1 agonist curdlan regulates osteoclastogenesis by inhibiting nuclear factor of activated T cells cytoplasmic 1 (NFATc1) through Syk kinase. J Biol Chem, 289(27):19191-203.
Sample Types: Whole Cells
Applications: ICC
Rappleye CA et al. (2007), Histoplasma capsulatum alpha-(1,3)-glucan blocks innate immune recognition by the beta-glucan receptor. Proc. Natl. Acad. Sci. U.S.A., 104(4):1366-70.
Applications: Neutralization
Fuller GL et al. (2007), The C-type lectin receptors CLEC-2 and Dectin-1, but not DC-SIGN, signal via a novel YXXL-dependent signaling cascade. J. Biol. Chem., 282(17):12397-409.
Product Documents for Mouse Dectin-1/CLEC7A Antibody