Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering.
Denver C Surrao, Stuart Skabo, Yu-Qian Chau, Jason Ionnis Limnios, Kinnari J. Shelat, Qin Liu
Clem Jones Centre for Regenerative Medicine
Research output: Contribution to conference › Abstract › Research › peer-review
Introduction: Age related macular degeneration (AMD) is the leading cause of irreversible vision loss in the developed world[1],[2] and manifests as either dry or wet AMD[1],[2]. In dry AMD, the retinal pigmented epithelium cells (RPEs) and Bruch's membrane (BM) are damaged, leading to photoreceptor dysfunction and death, and subsequent vision reduction and/or complete loss[2],[3]. Aim: The aim of this project was to develop a biomimetic scaffold for the treatment of dry AMD. Here, we focused on developing electrospun polymeric scaffolds, namely: poly(ε-caprolactone) (PCL), poly(L-lactide-co-glycolide) (PLGA), poly(D,L-lactide-co-L-lactide) (PDLLA) and poly(L-lactide) (PLLA) that mimicked the microenvironment of native BM. Additionally, we investigated the in vitro efficacy of these scaffolds to support the proliferation and functionality of human fetal RPE cells (hfRPEs). Materials and Methods: Electrospun membranes were fabricated from the above-mentioned polymers via electrospinning, as described previously[2],[4]. SEM was used to determine fibre diameter, scaffold thickness, and porosity of electrospun membranes. Surface roughness and surface stiffness were measured using AFM at 1 µm/s and a 5 nN load. In in vitro studies, hfRPEs were seeded (10,000/cm2) on electrospun membranes, which were then assessed at various time points via transepithelial electrical resistance (TER), SEM, q-PCR, and immunohistochemistry. Results and Discussion: Electrospun polymeric scaffolds with average fibre diameters of 70 nm, the first of its kind, and thicknesses < 1 μm, coupled with porosities > 40%, were fabricated via electrospinning, thereby mimicking the inner collagenous layer of native BM (thickness 1.4 μm and collagen fibre diameter 60-70 nm)[5] (Fig 1).
https://doi.org/10.3389/conf.FBIOE.2016.01.01616
Published - 30 Mar 2016
The 10th World Biomaterials Congress - Montreal, Canada
Conference number: 10th
http://www.wbc2016.org/
The 10th World Biomaterials Congress
Bruch Membrane
Polyglactin 910
Biomimetics
poly(lactide)
Surrao, D. C., Skabo, S., Chau, Y-Q., Limnios, J. I., Shelat, K. J., & Liu, Q. (2016). Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering.. Abstract from The 10th World Biomaterials Congress, Montreal, Canada. https://doi.org/10.3389/conf.FBIOE.2016.01.01616
Surrao, Denver C ; Skabo, Stuart ; Chau, Yu-Qian ; Limnios, Jason Ionnis ; Shelat, Kinnari J. ; Liu, Qin. / Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering. Abstract from The 10th World Biomaterials Congress, Montreal, Canada.
@conference{e8bb888194994a6f8de4884605b3729f,
title = "Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering.",
abstract = "Introduction: Age related macular degeneration (AMD) is the leading cause of irreversible vision loss in the developed world[1],[2] and manifests as either dry or wet AMD[1],[2]. In dry AMD, the retinal pigmented epithelium cells (RPEs) and Bruch's membrane (BM) are damaged, leading to photoreceptor dysfunction and death, and subsequent vision reduction and/or complete loss[2],[3]. Aim: The aim of this project was to develop a biomimetic scaffold for the treatment of dry AMD. Here, we focused on developing electrospun polymeric scaffolds, namely: poly(ε-caprolactone) (PCL), poly(L-lactide-co-glycolide) (PLGA), poly(D,L-lactide-co-L-lactide) (PDLLA) and poly(L-lactide) (PLLA) that mimicked the microenvironment of native BM. Additionally, we investigated the in vitro efficacy of these scaffolds to support the proliferation and functionality of human fetal RPE cells (hfRPEs). Materials and Methods: Electrospun membranes were fabricated from the above-mentioned polymers via electrospinning, as described previously[2],[4]. SEM was used to determine fibre diameter, scaffold thickness, and porosity of electrospun membranes. Surface roughness and surface stiffness were measured using AFM at 1 µm/s and a 5 nN load. In in vitro studies, hfRPEs were seeded (10,000/cm2) on electrospun membranes, which were then assessed at various time points via transepithelial electrical resistance (TER), SEM, q-PCR, and immunohistochemistry. Results and Discussion: Electrospun polymeric scaffolds with average fibre diameters of 70 nm, the first of its kind, and thicknesses < 1 μm, coupled with porosities > 40{\%}, were fabricated via electrospinning, thereby mimicking the inner collagenous layer of native BM (thickness 1.4 μm and collagen fibre diameter 60-70 nm)[5] (Fig 1).",
author = "Surrao, {Denver C} and Stuart Skabo and Yu-Qian Chau and Limnios, {Jason Ionnis} and Shelat, {Kinnari J.} and Qin Liu",
doi = "10.3389/conf.FBIOE.2016.01.01616",
note = "The 10th World Biomaterials Congress, WBC 2016 ; Conference date: 17-05-2016 Through 22-05-2016",
url = "http://www.wbc2016.org/",
Surrao, DC, Skabo, S, Chau, Y-Q, Limnios, JI, Shelat, KJ & Liu, Q 2016, 'Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering.' The 10th World Biomaterials Congress, Montreal, Canada, 17/05/16 - 22/05/16, . https://doi.org/10.3389/conf.FBIOE.2016.01.01616
Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering. / Surrao, Denver C; Skabo, Stuart; Chau, Yu-Qian; Limnios, Jason Ionnis; Shelat, Kinnari J.; Liu, Qin.
2016. Abstract from The 10th World Biomaterials Congress, Montreal, Canada.
T1 - Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering.
AU - Surrao, Denver C
AU - Skabo, Stuart
AU - Chau, Yu-Qian
AU - Limnios, Jason Ionnis
AU - Shelat, Kinnari J.
AU - Liu, Qin
N2 - Introduction: Age related macular degeneration (AMD) is the leading cause of irreversible vision loss in the developed world[1],[2] and manifests as either dry or wet AMD[1],[2]. In dry AMD, the retinal pigmented epithelium cells (RPEs) and Bruch's membrane (BM) are damaged, leading to photoreceptor dysfunction and death, and subsequent vision reduction and/or complete loss[2],[3]. Aim: The aim of this project was to develop a biomimetic scaffold for the treatment of dry AMD. Here, we focused on developing electrospun polymeric scaffolds, namely: poly(ε-caprolactone) (PCL), poly(L-lactide-co-glycolide) (PLGA), poly(D,L-lactide-co-L-lactide) (PDLLA) and poly(L-lactide) (PLLA) that mimicked the microenvironment of native BM. Additionally, we investigated the in vitro efficacy of these scaffolds to support the proliferation and functionality of human fetal RPE cells (hfRPEs). Materials and Methods: Electrospun membranes were fabricated from the above-mentioned polymers via electrospinning, as described previously[2],[4]. SEM was used to determine fibre diameter, scaffold thickness, and porosity of electrospun membranes. Surface roughness and surface stiffness were measured using AFM at 1 µm/s and a 5 nN load. In in vitro studies, hfRPEs were seeded (10,000/cm2) on electrospun membranes, which were then assessed at various time points via transepithelial electrical resistance (TER), SEM, q-PCR, and immunohistochemistry. Results and Discussion: Electrospun polymeric scaffolds with average fibre diameters of 70 nm, the first of its kind, and thicknesses < 1 μm, coupled with porosities > 40%, were fabricated via electrospinning, thereby mimicking the inner collagenous layer of native BM (thickness 1.4 μm and collagen fibre diameter 60-70 nm)[5] (Fig 1).
AB - Introduction: Age related macular degeneration (AMD) is the leading cause of irreversible vision loss in the developed world[1],[2] and manifests as either dry or wet AMD[1],[2]. In dry AMD, the retinal pigmented epithelium cells (RPEs) and Bruch's membrane (BM) are damaged, leading to photoreceptor dysfunction and death, and subsequent vision reduction and/or complete loss[2],[3]. Aim: The aim of this project was to develop a biomimetic scaffold for the treatment of dry AMD. Here, we focused on developing electrospun polymeric scaffolds, namely: poly(ε-caprolactone) (PCL), poly(L-lactide-co-glycolide) (PLGA), poly(D,L-lactide-co-L-lactide) (PDLLA) and poly(L-lactide) (PLLA) that mimicked the microenvironment of native BM. Additionally, we investigated the in vitro efficacy of these scaffolds to support the proliferation and functionality of human fetal RPE cells (hfRPEs). Materials and Methods: Electrospun membranes were fabricated from the above-mentioned polymers via electrospinning, as described previously[2],[4]. SEM was used to determine fibre diameter, scaffold thickness, and porosity of electrospun membranes. Surface roughness and surface stiffness were measured using AFM at 1 µm/s and a 5 nN load. In in vitro studies, hfRPEs were seeded (10,000/cm2) on electrospun membranes, which were then assessed at various time points via transepithelial electrical resistance (TER), SEM, q-PCR, and immunohistochemistry. Results and Discussion: Electrospun polymeric scaffolds with average fibre diameters of 70 nm, the first of its kind, and thicknesses < 1 μm, coupled with porosities > 40%, were fabricated via electrospinning, thereby mimicking the inner collagenous layer of native BM (thickness 1.4 μm and collagen fibre diameter 60-70 nm)[5] (Fig 1).
UR - https://www.frontiersin.org/10.3389/conf.FBIOE.2016.01.01616/event_abstract
UR - http://tech4pco.com/wbc2016/flipbook/WBC2016.pdf
UR - https://www.frontiersin.org/events/10th_World_Biomaterials_Congress/2893
U2 - 10.3389/conf.FBIOE.2016.01.01616
DO - 10.3389/conf.FBIOE.2016.01.01616
M3 - Abstract
Surrao DC, Skabo S, Chau Y-Q, Limnios JI, Shelat KJ, Liu Q. Design, development and in vitro evaluation of synthetic scaffolds for retinal tissue engineering.. 2016. Abstract from The 10th World Biomaterials Congress, Montreal, Canada. https://doi.org/10.3389/conf.FBIOE.2016.01.01616
10.3389/conf.FBIOE.2016.01.01616
Abstract published in 'Frontiers'
10th World Biometerials Congress - 'Frontiers'