Reduced expression of urokinase plasminogen activator in brown adipose tissue of obese mouse models
Chung Ze Wu, Li Chien Chang, Chao Wen Cheng, Te Chao Fang, Yuh Feng Lin, Dee Pei, Jin Shuen Chen
衛生福利部雙和醫院
內分泌暨新陳代謝科
國際醫學研究碩博士學位學程
臨床醫學研究所
臺北醫學大學泌尿腎臟研究中心
In recent decades, the obesity epidemic has resulted in morbidity and mortality rates increasing globally. In this study, using obese mouse models, we investigated the relationship among urokinase plasminogen activator (uPA), metabolic disorders, glomerular filtration rate, and adipose tissues. Two groups, each comprised of C57BL/6J and BALB/c male mice, were fed a chow diet (CD) and a high fat diet (HFD), respectively. Within the two HFD groups, half of each group were euthanized at 8 weeks (W8) or 16 weeks (W16). Blood, urine and adipose tissues were collected and harvested for evaluation of the effects of obesity. In both mouse models, triglyceride with insulin resistance and body weight increased with duration when fed a HFD in comparison to those in the groups on a CD. In both C57BL/6J and BALB/c HFD mice, levels of serum uPA initially increased significantly in the W8 group, and then the increment decreased in the W16 group. The glomerular filtration rate declined in both HFD groups. The expression of uPA significantly decreased in brown adipose tissue (BAT), but not in white adipose tissue, when compared with that in the CD group. The results suggest a decline in the expression of uPA in BAT in obese m models as the serum uPA increases. There is possibly an association with BAT fibrosis and dysfunction, which may need further study.
文章編號
International journal of molecular sciences
https://doi.org/10.3390/ijms22073407
已發佈 - 四月 1 2021
催化
分子生物學
光譜
電腦科學應用
物理與理論化學
有機化學
無機化學
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diets Physics & Astronomy 100%
adipose tissues Physics & Astronomy 94%
Nutrition Engineering & Materials Science 79%
Obese Mice Medicine & Life Sciences 77%
Brown Adipose Tissue Medicine & Life Sciences 77%
Plasminogen Activators Medicine & Life Sciences 74%
Urokinase-Type Plasminogen Activator Medicine & Life Sciences 71%
mice Physics & Astronomy 65%
Wu, C. Z., Chang, L. C., Cheng, C. W., Fang, T. C., Lin, Y. F., Pei, D., & Chen, J. S. (2021). Reduced expression of urokinase plasminogen activator in brown adipose tissue of obese mouse models. International journal of molecular sciences, 22(7), [3407]. https://doi.org/10.3390/ijms22073407
Reduced expression of urokinase plasminogen activator in brown adipose tissue of obese mouse models. / Wu, Chung Ze; Chang, Li Chien; Cheng, Chao Wen; Fang, Te Chao; Lin, Yuh Feng; Pei, Dee; Chen, Jin Shuen.
於: International journal of molecular sciences, 卷 22, 編號 7, 3407, 01.04.2021.
Wu, CZ, Chang, LC, Cheng, CW, Fang, TC, Lin, YF, Pei, D & Chen, JS 2021, 'Reduced expression of urokinase plasminogen activator in brown adipose tissue of obese mouse models', International journal of molecular sciences, 卷 22, 編號 7, 3407. https://doi.org/10.3390/ijms22073407
Wu CZ, Chang LC, Cheng CW, Fang TC, Lin YF, Pei D 等. Reduced expression of urokinase plasminogen activator in brown adipose tissue of obese mouse models. International journal of molecular sciences. 2021 4月 1;22(7). 3407. https://doi.org/10.3390/ijms22073407
Wu, Chung Ze ; Chang, Li Chien ; Cheng, Chao Wen ; Fang, Te Chao ; Lin, Yuh Feng ; Pei, Dee ; Chen, Jin Shuen. / Reduced expression of urokinase plasminogen activator in brown adipose tissue of obese mouse models. 於: International journal of molecular sciences. 2021 ; 卷 22, 編號 7.
@article{c83d75a9effc4bfb85806d132db94bf5,
title = "Reduced expression of urokinase plasminogen activator in brown adipose tissue of obese mouse models",
abstract = "In recent decades, the obesity epidemic has resulted in morbidity and mortality rates increasing globally. In this study, using obese mouse models, we investigated the relationship among urokinase plasminogen activator (uPA), metabolic disorders, glomerular filtration rate, and adipose tissues. Two groups, each comprised of C57BL/6J and BALB/c male mice, were fed a chow diet (CD) and a high fat diet (HFD), respectively. Within the two HFD groups, half of each group were euthanized at 8 weeks (W8) or 16 weeks (W16). Blood, urine and adipose tissues were collected and harvested for evaluation of the effects of obesity. In both mouse models, triglyceride with insulin resistance and body weight increased with duration when fed a HFD in comparison to those in the groups on a CD. In both C57BL/6J and BALB/c HFD mice, levels of serum uPA initially increased significantly in the W8 group, and then the increment decreased in the W16 group. The glomerular filtration rate declined in both HFD groups. The expression of uPA significantly decreased in brown adipose tissue (BAT), but not in white adipose tissue, when compared with that in the CD group. The results suggest a decline in the expression of uPA in BAT in obese m models as the serum uPA increases. There is possibly an association with BAT fibrosis and dysfunction, which may need further study.",
keywords = "Brown adipose tissue, Obesity, Urokinase plasminogen activator",
author = "Wu, {Chung Ze} and Chang, {Li Chien} and Cheng, {Chao Wen} and Fang, {Te Chao} and Lin, {Yuh Feng} and Dee Pei and Chen, {Jin Shuen}",
note = "Funding Information: This work was supported by National Defense Medical Center, Tri-Service General Hospital, Taiwan under Grant TSGH-C106-093, TSGH-C107-097, MAB-106-091 and Taipei Medical University, Taiwan under Grant TMU105-AE1-B28. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
doi = "10.3390/ijms22073407",
journal = "International Journal of Molecular Sciences",
publisher = "MDPI AG",
T1 - Reduced expression of urokinase plasminogen activator in brown adipose tissue of obese mouse models
AU - Wu, Chung Ze
AU - Chang, Li Chien
AU - Cheng, Chao Wen
AU - Fang, Te Chao
AU - Lin, Yuh Feng
AU - Pei, Dee
AU - Chen, Jin Shuen
N1 - Funding Information: This work was supported by National Defense Medical Center, Tri-Service General Hospital, Taiwan under Grant TSGH-C106-093, TSGH-C107-097, MAB-106-091 and Taipei Medical University, Taiwan under Grant TMU105-AE1-B28. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
N2 - In recent decades, the obesity epidemic has resulted in morbidity and mortality rates increasing globally. In this study, using obese mouse models, we investigated the relationship among urokinase plasminogen activator (uPA), metabolic disorders, glomerular filtration rate, and adipose tissues. Two groups, each comprised of C57BL/6J and BALB/c male mice, were fed a chow diet (CD) and a high fat diet (HFD), respectively. Within the two HFD groups, half of each group were euthanized at 8 weeks (W8) or 16 weeks (W16). Blood, urine and adipose tissues were collected and harvested for evaluation of the effects of obesity. In both mouse models, triglyceride with insulin resistance and body weight increased with duration when fed a HFD in comparison to those in the groups on a CD. In both C57BL/6J and BALB/c HFD mice, levels of serum uPA initially increased significantly in the W8 group, and then the increment decreased in the W16 group. The glomerular filtration rate declined in both HFD groups. The expression of uPA significantly decreased in brown adipose tissue (BAT), but not in white adipose tissue, when compared with that in the CD group. The results suggest a decline in the expression of uPA in BAT in obese m models as the serum uPA increases. There is possibly an association with BAT fibrosis and dysfunction, which may need further study.
AB - In recent decades, the obesity epidemic has resulted in morbidity and mortality rates increasing globally. In this study, using obese mouse models, we investigated the relationship among urokinase plasminogen activator (uPA), metabolic disorders, glomerular filtration rate, and adipose tissues. Two groups, each comprised of C57BL/6J and BALB/c male mice, were fed a chow diet (CD) and a high fat diet (HFD), respectively. Within the two HFD groups, half of each group were euthanized at 8 weeks (W8) or 16 weeks (W16). Blood, urine and adipose tissues were collected and harvested for evaluation of the effects of obesity. In both mouse models, triglyceride with insulin resistance and body weight increased with duration when fed a HFD in comparison to those in the groups on a CD. In both C57BL/6J and BALB/c HFD mice, levels of serum uPA initially increased significantly in the W8 group, and then the increment decreased in the W16 group. The glomerular filtration rate declined in both HFD groups. The expression of uPA significantly decreased in brown adipose tissue (BAT), but not in white adipose tissue, when compared with that in the CD group. The results suggest a decline in the expression of uPA in BAT in obese m models as the serum uPA increases. There is possibly an association with BAT fibrosis and dysfunction, which may need further study.
KW - Brown adipose tissue
KW - Obesity
KW - Urokinase plasminogen activator
U2 - 10.3390/ijms22073407
DO - 10.3390/ijms22073407
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
M1 - 3407