Fermented soybean liquid alleviated peptic ulcer through the destruction of acidic proton pump rather than suppression of urease of helicobacter pylori: A kinetic analysis
Hui Er Wang, Chi Huang Chang, Yaw Bee Ker, Chiung Chi Peng, Kuan Chou Chen, Robert Y. Peng
泌尿學科
衛生福利部雙和醫院
臨床醫學研究所
臺北醫學大學泌尿腎臟研究中心
Fermented soybean liquid (FSL) has been well cited for its broad spectrum of biological effects, yet its documented gastropeptic ulcer (GPU) ameliorating effect is still lacking. It was hypothesized that to avoid the injury exerted by gastric fluid, HP has to be sheltered in chyme emulsions immediately on infection. The HP urease (HPU) and the acidic proton pump (PP) may act as the two-point pH modulator to maintain an optimum pH between 6 and 7, and FSL is able to destroy such a modulating mechanism. FSL exhibited higher contents of isoflavonoids (2.5-17.3-fold) and essential amino acids (1.5-4.0-fold) than the nonfermented. FSL administered at 1 g/20 mL tid for 3 months eradicated Helicobacter pylori (HP) by 82% in 37 volunteers having GPU (p <0.20); simultaneously, the plasma conjugated diene and TBARs levels were significantly resumed (p <0.05). Kinetic analysis based on the conventional urease theory revealed that a cluster of 2.0 × 109 of HP cells is required for a single attack in the gastric lumen at pH 1.0-2.5. To verify the hypothesis, chyme-shelter testing was conducted in artificial gastric fluid (pH 2.4 ± 0.20). Results showed the HP cell viability was time- and size-dependent. At 20 min of contact time, the viability was 100, 4.2, 31.4, 43.3, 57.2, and 82.6%, respectively, in intact, dispersed, and particulate chymes (mesh sizes 80, 60, 40, and 20). The corresponding data became 96.2, 0.0, 14.5, 18.5, 21.3, and 28.6%, respectively, at a contact time of 40 min. Conclusively, the kinetic analysis and the chyme-shelter testing revealed that direct infection by bare HP cells is unlikely in real status. FSL is beneficial to GPU most probably due to its ability to raise blood alkalinity levels, destroying the PP and its ROS suppressing effect.
https://doi.org/10.1021/jf200770n
已發佈 - 6月 22 2011
化學 (全部)
農業與生物科學 (全部)
10.1021/jf200770n
深入研究「Fermented soybean liquid alleviated peptic ulcer through the destruction of acidic proton pump rather than suppression of urease of helicobacter pylori: A kinetic analysis」主題。共同形成了獨特的指紋。
peptic ulcers Agriculture & Biology 100%
Proton Pumps Medicine & Life Sciences 94%
proton pump Agriculture & Biology 86%
Urease Medicine & Life Sciences 84%
Soybeans Medicine & Life Sciences 83%
Helicobacter pylori Agriculture & Biology 81%
urease Agriculture & Biology 74%
Peptic Ulcer Medicine & Life Sciences 72%
Wang, H. E., Chang, C. H., Ker, Y. B., Peng, C. C., Chen, K. C., & Peng, R. Y. (2011). Fermented soybean liquid alleviated peptic ulcer through the destruction of acidic proton pump rather than suppression of urease of helicobacter pylori: A kinetic analysis. Journal of Agricultural and Food Chemistry, 59(12), 6730-6739. https://doi.org/10.1021/jf200770n
Fermented soybean liquid alleviated peptic ulcer through the destruction of acidic proton pump rather than suppression of urease of helicobacter pylori : A kinetic analysis. / Wang, Hui Er; Chang, Chi Huang; Ker, Yaw Bee 等.
於: Journal of Agricultural and Food Chemistry, 卷 59, 編號 12, 22.06.2011, p. 6730-6739.
Wang, HE, Chang, CH, Ker, YB, Peng, CC, Chen, KC & Peng, RY 2011, 'Fermented soybean liquid alleviated peptic ulcer through the destruction of acidic proton pump rather than suppression of urease of helicobacter pylori: A kinetic analysis', Journal of Agricultural and Food Chemistry, 卷 59, 編號 12, 頁 6730-6739. https://doi.org/10.1021/jf200770n
Wang HE, Chang CH, Ker YB, Peng CC, Chen KC, Peng RY. Fermented soybean liquid alleviated peptic ulcer through the destruction of acidic proton pump rather than suppression of urease of helicobacter pylori: A kinetic analysis. Journal of Agricultural and Food Chemistry. 2011 6月 22;59(12):6730-6739. doi: 10.1021/jf200770n
Wang, Hui Er ; Chang, Chi Huang ; Ker, Yaw Bee 等. / Fermented soybean liquid alleviated peptic ulcer through the destruction of acidic proton pump rather than suppression of urease of helicobacter pylori : A kinetic analysis. 於: Journal of Agricultural and Food Chemistry. 2011 ; 卷 59, 編號 12. 頁 6730-6739.
@article{52257244d4cb434ca06254061a4a9d20,
title = "Fermented soybean liquid alleviated peptic ulcer through the destruction of acidic proton pump rather than suppression of urease of helicobacter pylori: A kinetic analysis",
abstract = "Fermented soybean liquid (FSL) has been well cited for its broad spectrum of biological effects, yet its documented gastropeptic ulcer (GPU) ameliorating effect is still lacking. It was hypothesized that to avoid the injury exerted by gastric fluid, HP has to be sheltered in chyme emulsions immediately on infection. The HP urease (HPU) and the acidic proton pump (PP) may act as the two-point pH modulator to maintain an optimum pH between 6 and 7, and FSL is able to destroy such a modulating mechanism. FSL exhibited higher contents of isoflavonoids (2.5-17.3-fold) and essential amino acids (1.5-4.0-fold) than the nonfermented. FSL administered at 1 g/20 mL tid for 3 months eradicated Helicobacter pylori (HP) by 82% in 37 volunteers having GPU (p <0.20); simultaneously, the plasma conjugated diene and TBARs levels were significantly resumed (p <0.05). Kinetic analysis based on the conventional urease theory revealed that a cluster of 2.0 × 109 of HP cells is required for a single attack in the gastric lumen at pH 1.0-2.5. To verify the hypothesis, chyme-shelter testing was conducted in artificial gastric fluid (pH 2.4 ± 0.20). Results showed the HP cell viability was time- and size-dependent. At 20 min of contact time, the viability was 100, 4.2, 31.4, 43.3, 57.2, and 82.6%, respectively, in intact, dispersed, and particulate chymes (mesh sizes 80, 60, 40, and 20). The corresponding data became 96.2, 0.0, 14.5, 18.5, 21.3, and 28.6%, respectively, at a contact time of 40 min. Conclusively, the kinetic analysis and the chyme-shelter testing revealed that direct infection by bare HP cells is unlikely in real status. FSL is beneficial to GPU most probably due to its ability to raise blood alkalinity levels, destroying the PP and its ROS suppressing effect.",
keywords = "H. pylori urease, fermented soybean liquid, isoflavonoids, peptic ulcer, proton pump",
author = "Wang, {Hui Er} and Chang, {Chi Huang} and Ker, {Yaw Bee} and Peng, {Chiung Chi} and Chen, {Kuan Chou} and Peng, {Robert Y.}",
doi = "10.1021/jf200770n",
journal = "Journal of Agricultural and Food Chemistry",
T1 - Fermented soybean liquid alleviated peptic ulcer through the destruction of acidic proton pump rather than suppression of urease of helicobacter pylori
T2 - A kinetic analysis
AU - Wang, Hui Er
AU - Chang, Chi Huang
AU - Ker, Yaw Bee
AU - Peng, Chiung Chi
AU - Chen, Kuan Chou
AU - Peng, Robert Y.
N2 - Fermented soybean liquid (FSL) has been well cited for its broad spectrum of biological effects, yet its documented gastropeptic ulcer (GPU) ameliorating effect is still lacking. It was hypothesized that to avoid the injury exerted by gastric fluid, HP has to be sheltered in chyme emulsions immediately on infection. The HP urease (HPU) and the acidic proton pump (PP) may act as the two-point pH modulator to maintain an optimum pH between 6 and 7, and FSL is able to destroy such a modulating mechanism. FSL exhibited higher contents of isoflavonoids (2.5-17.3-fold) and essential amino acids (1.5-4.0-fold) than the nonfermented. FSL administered at 1 g/20 mL tid for 3 months eradicated Helicobacter pylori (HP) by 82% in 37 volunteers having GPU (p <0.20); simultaneously, the plasma conjugated diene and TBARs levels were significantly resumed (p <0.05). Kinetic analysis based on the conventional urease theory revealed that a cluster of 2.0 × 109 of HP cells is required for a single attack in the gastric lumen at pH 1.0-2.5. To verify the hypothesis, chyme-shelter testing was conducted in artificial gastric fluid (pH 2.4 ± 0.20). Results showed the HP cell viability was time- and size-dependent. At 20 min of contact time, the viability was 100, 4.2, 31.4, 43.3, 57.2, and 82.6%, respectively, in intact, dispersed, and particulate chymes (mesh sizes 80, 60, 40, and 20). The corresponding data became 96.2, 0.0, 14.5, 18.5, 21.3, and 28.6%, respectively, at a contact time of 40 min. Conclusively, the kinetic analysis and the chyme-shelter testing revealed that direct infection by bare HP cells is unlikely in real status. FSL is beneficial to GPU most probably due to its ability to raise blood alkalinity levels, destroying the PP and its ROS suppressing effect.
AB - Fermented soybean liquid (FSL) has been well cited for its broad spectrum of biological effects, yet its documented gastropeptic ulcer (GPU) ameliorating effect is still lacking. It was hypothesized that to avoid the injury exerted by gastric fluid, HP has to be sheltered in chyme emulsions immediately on infection. The HP urease (HPU) and the acidic proton pump (PP) may act as the two-point pH modulator to maintain an optimum pH between 6 and 7, and FSL is able to destroy such a modulating mechanism. FSL exhibited higher contents of isoflavonoids (2.5-17.3-fold) and essential amino acids (1.5-4.0-fold) than the nonfermented. FSL administered at 1 g/20 mL tid for 3 months eradicated Helicobacter pylori (HP) by 82% in 37 volunteers having GPU (p <0.20); simultaneously, the plasma conjugated diene and TBARs levels were significantly resumed (p <0.05). Kinetic analysis based on the conventional urease theory revealed that a cluster of 2.0 × 109 of HP cells is required for a single attack in the gastric lumen at pH 1.0-2.5. To verify the hypothesis, chyme-shelter testing was conducted in artificial gastric fluid (pH 2.4 ± 0.20). Results showed the HP cell viability was time- and size-dependent. At 20 min of contact time, the viability was 100, 4.2, 31.4, 43.3, 57.2, and 82.6%, respectively, in intact, dispersed, and particulate chymes (mesh sizes 80, 60, 40, and 20). The corresponding data became 96.2, 0.0, 14.5, 18.5, 21.3, and 28.6%, respectively, at a contact time of 40 min. Conclusively, the kinetic analysis and the chyme-shelter testing revealed that direct infection by bare HP cells is unlikely in real status. FSL is beneficial to GPU most probably due to its ability to raise blood alkalinity levels, destroying the PP and its ROS suppressing effect.
KW - H. pylori urease
KW - fermented soybean liquid
KW - isoflavonoids
KW - peptic ulcer
KW - proton pump
U2 - 10.1021/jf200770n
DO - 10.1021/jf200770n
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry