Removal of cholera toxin from aqueous solution by probiotic bacteria
Jari E. Heikkilä, Sonja M.K. Nybom, Seppo J. Salminen, Jussi A.O. Meriluoto
Åbo Akademi University
Laboratoryservices
Cholera remains a serious health problem, especially in developing countries where basic hygiene standards are not met. The symptoms of cholera are caused by cholera toxin, an enterotoxin, which is produced by the bacterium Vibrio cholerae. We have recently shown that human probiotic bacteria are capable of removing cyanobacterial toxins from aqueous solutions. In the present study we investigate the ability of the human probiotic bacteria, Lactobacillus rhamnosus strain GG (ATCC 53103) and Bifidobacterium longum 46 (DSM 14583), to remove cholera toxin from solution in vitro. Lactobacillus rhamnosus strain GG and Bifidobacterium longum 46 were able to remove 68% and 59% of cholera toxin from aqueous solutions during 18 h of incubation at 37 °C, respectively. The effect was dependent on bacterial concentration and L. rhamnosus GG was more effective at lower bacterial concentrations. No significant effect on cholera toxin concentration was observed when nonviable bacteria or bacterial supernatant was used.
https://doi.org/10.3390/ph5060665
Probiotic bacteria
10.3390/ph5060665
Fingerprint Dive into the research topics of 'Removal of cholera toxin from aqueous solution by probiotic bacteria'. Together they form a unique fingerprint.
Lactobacillus rhamnosus Medicine & Life Sciences
Bifidobacterium longum Medicine & Life Sciences
Bacteria Medicine & Life Sciences
Cholera Medicine & Life Sciences
cyanobacterial toxin Medicine & Life Sciences
Vibrio cholerae stN protein Medicine & Life Sciences
Heikkilä, J. E., Nybom, S. M. K., Salminen, S. J., & Meriluoto, J. A. O. (2012). Removal of cholera toxin from aqueous solution by probiotic bacteria. Pharmaceuticals, 5(6), 665-673. https://doi.org/10.3390/ph5060665
Heikkilä, Jari E. ; Nybom, Sonja M.K. ; Salminen, Seppo J. ; Meriluoto, Jussi A.O. / Removal of cholera toxin from aqueous solution by probiotic bacteria. In: Pharmaceuticals. 2012 ; Vol. 5, No. 6. pp. 665-673.
@article{a8786928ae2840debed26b52963a96da,
title = "Removal of cholera toxin from aqueous solution by probiotic bacteria",
abstract = "Cholera remains a serious health problem, especially in developing countries where basic hygiene standards are not met. The symptoms of cholera are caused by cholera toxin, an enterotoxin, which is produced by the bacterium Vibrio cholerae. We have recently shown that human probiotic bacteria are capable of removing cyanobacterial toxins from aqueous solutions. In the present study we investigate the ability of the human probiotic bacteria, Lactobacillus rhamnosus strain GG (ATCC 53103) and Bifidobacterium longum 46 (DSM 14583), to remove cholera toxin from solution in vitro. Lactobacillus rhamnosus strain GG and Bifidobacterium longum 46 were able to remove 68% and 59% of cholera toxin from aqueous solutions during 18 h of incubation at 37 °C, respectively. The effect was dependent on bacterial concentration and L. rhamnosus GG was more effective at lower bacterial concentrations. No significant effect on cholera toxin concentration was observed when nonviable bacteria or bacterial supernatant was used.",
keywords = "Bifidobacterium, Cholera toxin, Lactobacillus, Probiotic bacteria",
author = "Heikkil{\"a}, {Jari E.} and Nybom, {Sonja M.K.} and Salminen, {Seppo J.} and Meriluoto, {Jussi A.O.}",
doi = "10.3390/ph5060665",
journal = "Pharmaceuticals",
publisher = "MDPI",
Heikkilä, JE, Nybom, SMK, Salminen, SJ & Meriluoto, JAO 2012, 'Removal of cholera toxin from aqueous solution by probiotic bacteria', Pharmaceuticals, vol. 5, no. 6, pp. 665-673. https://doi.org/10.3390/ph5060665
Removal of cholera toxin from aqueous solution by probiotic bacteria. / Heikkilä, Jari E.; Nybom, Sonja M.K.; Salminen, Seppo J.; Meriluoto, Jussi A.O.
In: Pharmaceuticals, Vol. 5, No. 6, 15.06.2012, p. 665-673.
T1 - Removal of cholera toxin from aqueous solution by probiotic bacteria
AU - Heikkilä, Jari E.
AU - Nybom, Sonja M.K.
AU - Salminen, Seppo J.
AU - Meriluoto, Jussi A.O.
N2 - Cholera remains a serious health problem, especially in developing countries where basic hygiene standards are not met. The symptoms of cholera are caused by cholera toxin, an enterotoxin, which is produced by the bacterium Vibrio cholerae. We have recently shown that human probiotic bacteria are capable of removing cyanobacterial toxins from aqueous solutions. In the present study we investigate the ability of the human probiotic bacteria, Lactobacillus rhamnosus strain GG (ATCC 53103) and Bifidobacterium longum 46 (DSM 14583), to remove cholera toxin from solution in vitro. Lactobacillus rhamnosus strain GG and Bifidobacterium longum 46 were able to remove 68% and 59% of cholera toxin from aqueous solutions during 18 h of incubation at 37 °C, respectively. The effect was dependent on bacterial concentration and L. rhamnosus GG was more effective at lower bacterial concentrations. No significant effect on cholera toxin concentration was observed when nonviable bacteria or bacterial supernatant was used.
AB - Cholera remains a serious health problem, especially in developing countries where basic hygiene standards are not met. The symptoms of cholera are caused by cholera toxin, an enterotoxin, which is produced by the bacterium Vibrio cholerae. We have recently shown that human probiotic bacteria are capable of removing cyanobacterial toxins from aqueous solutions. In the present study we investigate the ability of the human probiotic bacteria, Lactobacillus rhamnosus strain GG (ATCC 53103) and Bifidobacterium longum 46 (DSM 14583), to remove cholera toxin from solution in vitro. Lactobacillus rhamnosus strain GG and Bifidobacterium longum 46 were able to remove 68% and 59% of cholera toxin from aqueous solutions during 18 h of incubation at 37 °C, respectively. The effect was dependent on bacterial concentration and L. rhamnosus GG was more effective at lower bacterial concentrations. No significant effect on cholera toxin concentration was observed when nonviable bacteria or bacterial supernatant was used.
KW - Bifidobacterium
KW - Cholera toxin
KW - Probiotic bacteria
U2 - 10.3390/ph5060665
DO - 10.3390/ph5060665
JO - Pharmaceuticals
JF - Pharmaceuticals
Heikkilä JE, Nybom SMK, Salminen SJ, Meriluoto JAO. Removal of cholera toxin from aqueous solution by probiotic bacteria. Pharmaceuticals. 2012 Jun 15;5(6):665-673. https://doi.org/10.3390/ph5060665
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