35 structures 326 species 1 interaction 439 sequences 37 architectures
Family: Glyco_hydro_48 (PF02011)
Summary: Glycosyl hydrolase family 48
Wikipedia: Glycoside hydrolase family 48
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Glycoside hydrolase family 48 Edit Wikipedia article
Glyco_hydro_48
x-tal structure of the mutant e44q of the cellulase cel48f in complex with a thiooligosaccharide
1fce
CAZy
In molecular biology, glycoside hydrolase family 48 is a family of glycoside hydrolases.
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy web site,[4][5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6][7]
Glycoside hydrolase family 48 CAZY GH_48 comprises enzymes with several known activities; endoglucanase (EC 3.2.1.4); cellobiohydrolase (EC 3.2.1.91).
An example of an enzyme containing a domain belonging to this family is one of the cellulases (celA) from the genome of the thermophilic anaerobic bacterium Caldocellum saccharolyticum. The celA gene product is a polypeptide of 1751 amino acids; this has a multidomain structure comprising two catalytic domains and two cellulose-binding domains, linked by Pro-Thr-rich regions. The N-terminal domain encodes an endoglucanase activity on carboxymethylcellulose, consistent with its similarity to several endo-1, 4-beta-D-glucanase sequences, and is a member of the glycoside hydrolase family 9. The C-terminal domain belongs to this family shows similarity to a cellulase from Clostridium thermocellum (CelS), which acts synergistically with a second component to hydrolyse crystalline cellulose.[8]
^ Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G (July 1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America. 92 (15): 7090–4. doi:10.1073/pnas.92.15.7090. PMC 41477  . PMID 7624375.
^ Davies G, Henrissat B (September 1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–9. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
^ Henrissat B, Bairoch A (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal. 316 ( Pt 2) (Pt 2): 695–6. PMC 1217404  . PMID 8687420.
^ "Home". CAZy.org. Retrieved 2018-03-06.
^ Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B (January 2014). "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research. 42 (Database issue): D490–5. doi:10.1093/nar/gkt1178. PMID 24270786.
^ "Glycoside Hydrolase Family 48". CAZypedia.org. Retrieved 2018-03-06.
^ "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes". Glycobiology. 28 (1): 3–8. December 2018. doi:10.1093/glycob/cwx089. PMID 29040563.
^ Te'o VS, Saul DJ, Bergquist PL (1995). "celA, another gene coding for a multidomain cellulase from the extreme thermophile Caldocellum saccharolyticum". Applied Microbiology and Biotechnology. 43 (2): 291–6. doi:10.1007/BF00172827. PMID 7612247.
Hydrolase: sugar hydrolases (EC 3.2)
3.2.1: Glycoside hydrolases
Disaccharidase
Sucrase/Sucrase-isomaltase/Invertase
Maltase
Trehalase
Lactase
Glucosidases
Cellulase
Alpha-glucosidase
Neutral AB
Neutral C
Beta-glucosidase
cytosolic
Debranching enzyme
Amylase
Alpha-amylase
Chitinase
Neuraminidase
NEU1
Bacterial neuraminidase
Viral neuraminidase
Galactosidases
alpha-Mannosidase
Glucuronidase
Pullulanase
Glucosylceramidase
lysosomal
non-lysosomal
Galactosylceramidase
Alpha-N-acetylgalactosaminidase
Alpha-N-acetylglucosaminidase
Fucosidase
Hexosaminidase
HEXB
Iduronidase
Maltase-glucoamylase
Heparanase
HPSE2
3.2.2: Hydrolysing
N-Glycosyl compounds
DNA glycosylases: Oxoguanine glycosylase
Glycosyl hydrolase family 48 Provide feedback
Members of this family are endoglucanase EC:3.2.1.4 and exoglucanase EC:3.2.1.91 enzymes that cleave cellulose or related substrate.
CAZY: GH48
PRINTS: PR00844
SCOP: 1fce
O-Glycosyl hydrolases (EC) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [PUBMED:7624375, PUBMED:8535779]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site.
Glycoside hydrolase family 48 CAZY comprises enzymes with several known activities; endoglucanase (EC); cellobiohydrolase (EC).
The largest cellulase gene sequenced to date is one of the cellulases (celA) from the genome of the thermophilic anaerobic bacterium Caldocellum saccharolyticum. The celA gene product is a polypeptide of 1751 amino acids; this has a multidomain structure comprising two catalytic domains and two cellulose-binding domains, linked by Pro-Thr-rich regions. The N-terminal domain encodes an endoglucanase activity on carboxymethylcellulose, consistent with its similarity to several endo-1, 4-beta-D-glucanase sequences. The C-terminal domain shows similarity to a cellulase from Clostridium thermocellum (CelS), which acts synergistically with a second component to hydrolyse crystalline cellulose [PUBMED:7612247].
Molecular function hydrolase activity, hydrolyzing O-glycosyl compounds (GO:0004553)
cellulase activity (GO:0008810)
Biological process cellulose catabolic process (GO:0030245)
This family is a member of clan 6_Hairpin (CL0059), which has the following description:
This Clan includes CAZy clans GH-L, GH-M and GH-G. The members of this clan share a common structure composed of 6 helical hairpins. Most members of this superfamily are glycosyl hydrolase enzymes.
Bac_rhamnosid6H C5-epim_C Cobalamin_bind DUF608 GDE_C GlcNAc_2-epim Glyco_hydro81C Glyco_hydro_100 Glyco_hydro_125 Glyco_hydro_127 Glyco_hydro_15 Glyco_hydro_36 Glyco_hydro_47 Glyco_hydro_48 Glyco_hydro_63 Glyco_hydro_65m Glyco_hydro_76 Glyco_hydro_8 Glyco_hydro_88 Glyco_hydro_9 Glycoamylase LANC_like Ldi Pec_lyase Prenyltrans SQHop_cyclase_C SQHop_cyclase_N TED_complement Trehalase
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Seed source: SwissProt
Number in full: 439
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Glyco_hydro_48 domain has been found. There are 35 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.