Improvement of ethanol yield from glycerol via conversion of pyruvate to ethanol in metabolically engineered Saccharomyces cerevisiae
Kyung Ok Yu, Ju Jung, Ahmad Bazli Ramzi, Seung Wook Kim, Chulhwan Park, Sung Ok Han
Department of Chemical and Biological Engineering
The conversion of low-priced glycerol to higher value products has been proposed as a way to improve the economic viability of the biofuels industry. In a previous study, the conversion of glycerol to ethanol in a metabolically engineered strain of Saccharomyces cerevisiae was accomplished by minimizing the synthesis of glycerol, the main by-product in ethanol fermentation processing. To further improve ethanol production, overexpression of the native genes involved in conversion of pyruvate to ethanol in S. cerevisiae was successfully accomplished. The overexpression of an alcohol dehydrogenase (adh1) and a pyruvate decarboxylase (pdc1) caused an increase in growth rate and glycerol consumption under fermentative conditions, which led to a slight increase of the final ethanol yield. The overall expression of the adh1 and pdc1 genes in the modified strains, combined with the lack of the fps1 and gpd2 genes, resulted in a 1.4-fold increase (about 5.4 g/L ethanol produced) in fps1 Δgpd2Δ(pGcyaDak, pGupCas) (about 4.0 g/L ethanol produced). In summary, it is possible to improve the ethanol yield by overexpression of the genes involved in the conversion of pyruvate to ethanol in engineered S. cerevisiae using glycerol as substrate.
Applied Biochemistry and Biotechnology
Published - 2012 Feb
Alcohol dehydrogenase
Pyruvate decarboxylase
Fingerprint Dive into the research topics of 'Improvement of ethanol yield from glycerol via conversion of pyruvate to ethanol in metabolically engineered Saccharomyces cerevisiae'. Together they form a unique fingerprint.
Glycerol Engineering & Materials Science
Yeast Chemical Compounds
Pyruvic Acid Chemical Compounds
Ethanol Engineering & Materials Science
Saccharomyces cerevisiae Medicine & Life Sciences
Pyruvate Decarboxylase Chemical Compounds
Oxidoreductases Engineering & Materials Science
Yu, K. O., Jung, J., Ramzi, A. B., Kim, S. W., Park, C., & Han, S. O. (2012). Improvement of ethanol yield from glycerol via conversion of pyruvate to ethanol in metabolically engineered Saccharomyces cerevisiae. Applied Biochemistry and Biotechnology, 166(4), 856-865. https://doi.org/10.1007/s12010-011-9475-9
Improvement of ethanol yield from glycerol via conversion of pyruvate to ethanol in metabolically engineered Saccharomyces cerevisiae. / Yu, Kyung Ok; Jung, Ju; Ramzi, Ahmad Bazli; Kim, Seung Wook; Park, Chulhwan; Han, Sung Ok.
In: Applied Biochemistry and Biotechnology, Vol. 166, No. 4, 02.2012, p. 856-865.
Yu, KO, Jung, J, Ramzi, AB, Kim, SW, Park, C & Han, SO 2012, 'Improvement of ethanol yield from glycerol via conversion of pyruvate to ethanol in metabolically engineered Saccharomyces cerevisiae', Applied Biochemistry and Biotechnology, vol. 166, no. 4, pp. 856-865. https://doi.org/10.1007/s12010-011-9475-9
Yu KO, Jung J, Ramzi AB, Kim SW, Park C, Han SO. Improvement of ethanol yield from glycerol via conversion of pyruvate to ethanol in metabolically engineered Saccharomyces cerevisiae. Applied Biochemistry and Biotechnology. 2012 Feb;166(4):856-865. https://doi.org/10.1007/s12010-011-9475-9
Yu, Kyung Ok ; Jung, Ju ; Ramzi, Ahmad Bazli ; Kim, Seung Wook ; Park, Chulhwan ; Han, Sung Ok. / Improvement of ethanol yield from glycerol via conversion of pyruvate to ethanol in metabolically engineered Saccharomyces cerevisiae. In: Applied Biochemistry and Biotechnology. 2012 ; Vol. 166, No. 4. pp. 856-865.
@article{f141a519123b4f5d88dbdb6a796ccb91,
title = "Improvement of ethanol yield from glycerol via conversion of pyruvate to ethanol in metabolically engineered Saccharomyces cerevisiae",
abstract = "The conversion of low-priced glycerol to higher value products has been proposed as a way to improve the economic viability of the biofuels industry. In a previous study, the conversion of glycerol to ethanol in a metabolically engineered strain of Saccharomyces cerevisiae was accomplished by minimizing the synthesis of glycerol, the main by-product in ethanol fermentation processing. To further improve ethanol production, overexpression of the native genes involved in conversion of pyruvate to ethanol in S. cerevisiae was successfully accomplished. The overexpression of an alcohol dehydrogenase (adh1) and a pyruvate decarboxylase (pdc1) caused an increase in growth rate and glycerol consumption under fermentative conditions, which led to a slight increase of the final ethanol yield. The overall expression of the adh1 and pdc1 genes in the modified strains, combined with the lack of the fps1 and gpd2 genes, resulted in a 1.4-fold increase (about 5.4 g/L ethanol produced) in fps1 Δgpd2Δ(pGcyaDak, pGupCas) (about 4.0 g/L ethanol produced). In summary, it is possible to improve the ethanol yield by overexpression of the genes involved in the conversion of pyruvate to ethanol in engineered S. cerevisiae using glycerol as substrate.",
keywords = "Alcohol dehydrogenase, Ethanol production, Glycerol, Pyruvate decarboxylase, Saccharomyces cerevisiae",
author = "Yu, {Kyung Ok} and Ju Jung and Ramzi, {Ahmad Bazli} and Kim, {Seung Wook} and Chulhwan Park and Han, {Sung Ok}",
journal = "Applied Biochemistry and Biotechnology",
publisher = "Humana Press",
T1 - Improvement of ethanol yield from glycerol via conversion of pyruvate to ethanol in metabolically engineered Saccharomyces cerevisiae
AU - Yu, Kyung Ok
AU - Jung, Ju
AU - Ramzi, Ahmad Bazli
AU - Kim, Seung Wook
AU - Park, Chulhwan
AU - Han, Sung Ok
N2 - The conversion of low-priced glycerol to higher value products has been proposed as a way to improve the economic viability of the biofuels industry. In a previous study, the conversion of glycerol to ethanol in a metabolically engineered strain of Saccharomyces cerevisiae was accomplished by minimizing the synthesis of glycerol, the main by-product in ethanol fermentation processing. To further improve ethanol production, overexpression of the native genes involved in conversion of pyruvate to ethanol in S. cerevisiae was successfully accomplished. The overexpression of an alcohol dehydrogenase (adh1) and a pyruvate decarboxylase (pdc1) caused an increase in growth rate and glycerol consumption under fermentative conditions, which led to a slight increase of the final ethanol yield. The overall expression of the adh1 and pdc1 genes in the modified strains, combined with the lack of the fps1 and gpd2 genes, resulted in a 1.4-fold increase (about 5.4 g/L ethanol produced) in fps1 Δgpd2Δ(pGcyaDak, pGupCas) (about 4.0 g/L ethanol produced). In summary, it is possible to improve the ethanol yield by overexpression of the genes involved in the conversion of pyruvate to ethanol in engineered S. cerevisiae using glycerol as substrate.
AB - The conversion of low-priced glycerol to higher value products has been proposed as a way to improve the economic viability of the biofuels industry. In a previous study, the conversion of glycerol to ethanol in a metabolically engineered strain of Saccharomyces cerevisiae was accomplished by minimizing the synthesis of glycerol, the main by-product in ethanol fermentation processing. To further improve ethanol production, overexpression of the native genes involved in conversion of pyruvate to ethanol in S. cerevisiae was successfully accomplished. The overexpression of an alcohol dehydrogenase (adh1) and a pyruvate decarboxylase (pdc1) caused an increase in growth rate and glycerol consumption under fermentative conditions, which led to a slight increase of the final ethanol yield. The overall expression of the adh1 and pdc1 genes in the modified strains, combined with the lack of the fps1 and gpd2 genes, resulted in a 1.4-fold increase (about 5.4 g/L ethanol produced) in fps1 Δgpd2Δ(pGcyaDak, pGupCas) (about 4.0 g/L ethanol produced). In summary, it is possible to improve the ethanol yield by overexpression of the genes involved in the conversion of pyruvate to ethanol in engineered S. cerevisiae using glycerol as substrate.
KW - Alcohol dehydrogenase
KW - Ethanol production
KW - Glycerol
KW - Pyruvate decarboxylase
KW - Saccharomyces cerevisiae
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology