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Determination of sildenafil and vardenafil by capillary zone electrophoresis using capacitively coupled contactless conductivity detection
Denis Tadeu Rajh Vidal,a Marcio Antonio Augellib and Claudimir Lucio do Lago*a
* Corresponding authors
a Departamento de Química Fundamental, Univerisidade de São Paulo, Av. Prof. Lineu Prestes, 748, São Paulo – SP, Brazil
E-mail: claudemi@iq.usp.br
Fax: +55 113091 3781
b Universidade Estadual Paulista - UNESP, São Paulo – SP, Brazil
A method based on capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) for determination of two important phosphodiesterase type-5 inhibitors (sildenafil and vardenafil) is introduced. The background electrolyte (BGE) consisted of an aqueous solution of 500 mmol L−1 acetic acid, and the capillary was previously treated with polybrene solution to prevent cationic analytes from adsorbing onto the inner surface. Although the analytes migrate in the counter flow, the total time is short. An instrument with two C4D detectors allowed a seamless transition from a fast method (less than one minute) but of low-efficiency using the first detector to a more efficient method using the second detector. The analysis of commercial tablets showed no significant difference between CE-C4D and HPLC methods. Conductivity detection is a well-known low selectivity detection scheme, which in conjunction with the high mobility of the co-ion in the BGE (hydroxonium) allows one to predict that other cationic analogues of sildenafil can also be detected. This is an interesting feature given the increasing number of compounds in this class.
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The article was received on 29 Nov 2012, accepted on 14 Feb 2013 and first published on 14 Feb 2013
Article type: Paper
DOI: 10.1039/C3AY26470J
Download Citation: Anal. Methods, 2013,5, 2041-2045
D. T. Rajh Vidal, M. A. Augelli and C. Lucio do Lago, Anal. Methods, 2013, 5, 2041
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Denis Tadeu Rajh Vidal
Marcio Antonio Augelli
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