Regional differences in serotonergic input to canine parasternal intercostal motoneurons
Wen Zhi Zhan, Carlos B. Mantilla, Phillip Zhan, Asaf Bitton, Y. S. Prakash, Andre De Troyer, Gary C. Sieck
Physiology & Biomedical Engineering
There is a mediolateral gradient in activation of the parasternal intercostal (PI) muscle during inspiration. In the present study, we tested the hypotheses that serotonergic [5-hydroxytryptamine (5-HT)] input from descending central drive and/or intrinsic size-related properties of PI motoneurons leads to the differential activation of PI muscles. In dogs, PI motoneurons innervating the medial and lateral regions of the PI muscles at the T3-T5 interspaces were retrogradely labeled by intramuscular injection of cholera toxin B subunit. After a 10-day survival period, PI motoneurons and 5-HT terminals were visualized by using immunohistochemistry and confocal imaging. There were no differences in motoneuron morphology among motoneurons innervating the medial and lateral regions of the PI muscle. However, the number of 5-HT terminals and the 5-HT terminal density (normalized for surface area) were greater in motoneurons innervating the medial region of the PI muscle compared with the lateral region. These results suggest that differences in distribution of 5-HT input may contribute to regional differences in PI muscle activation during inspiration and that differences in PI motoneuron recruitment do not relate to size.
Journal of applied physiology
https://doi.org/10.1152/jappl.2000.88.5.1581
Respiratory muscles
10.1152/jappl.2000.88.5.1581
Dive into the research topics of 'Regional differences in serotonergic input to canine parasternal intercostal motoneurons'. Together they form a unique fingerprint.
Intercostal Muscles Medicine & Life Sciences 100%
Canidae Medicine & Life Sciences 69%
Serotonin Medicine & Life Sciences 49%
Cholera Toxin Medicine & Life Sciences 13%
Intramuscular Injections Medicine & Life Sciences 12%
Dogs Medicine & Life Sciences 8%
Immunohistochemistry Medicine & Life Sciences 7%
Zhan, W. Z., Mantilla, C. B., Zhan, P., Bitton, A., Prakash, Y. S., De Troyer, A., & Sieck, G. C. (2000). Regional differences in serotonergic input to canine parasternal intercostal motoneurons. Journal of applied physiology, 88(5), 1581-1589. https://doi.org/10.1152/jappl.2000.88.5.1581
Regional differences in serotonergic input to canine parasternal intercostal motoneurons. / Zhan, Wen Zhi; Mantilla, Carlos B.; Zhan, Phillip et al.
In: Journal of applied physiology, Vol. 88, No. 5, 05.2000, p. 1581-1589.
Zhan, WZ, Mantilla, CB, Zhan, P, Bitton, A, Prakash, YS, De Troyer, A & Sieck, GC 2000, 'Regional differences in serotonergic input to canine parasternal intercostal motoneurons', Journal of applied physiology, vol. 88, no. 5, pp. 1581-1589. https://doi.org/10.1152/jappl.2000.88.5.1581
Zhan WZ, Mantilla CB, Zhan P, Bitton A, Prakash YS, De Troyer A et al. Regional differences in serotonergic input to canine parasternal intercostal motoneurons. Journal of applied physiology. 2000 May;88(5):1581-1589. doi: 10.1152/jappl.2000.88.5.1581
Zhan, Wen Zhi ; Mantilla, Carlos B. ; Zhan, Phillip et al. / Regional differences in serotonergic input to canine parasternal intercostal motoneurons. In: Journal of applied physiology. 2000 ; Vol. 88, No. 5. pp. 1581-1589.
@article{fcb726293f8f40d399d41d5094095621,
title = "Regional differences in serotonergic input to canine parasternal intercostal motoneurons",
abstract = "There is a mediolateral gradient in activation of the parasternal intercostal (PI) muscle during inspiration. In the present study, we tested the hypotheses that serotonergic [5-hydroxytryptamine (5-HT)] input from descending central drive and/or intrinsic size-related properties of PI motoneurons leads to the differential activation of PI muscles. In dogs, PI motoneurons innervating the medial and lateral regions of the PI muscles at the T3-T5 interspaces were retrogradely labeled by intramuscular injection of cholera toxin B subunit. After a 10-day survival period, PI motoneurons and 5-HT terminals were visualized by using immunohistochemistry and confocal imaging. There were no differences in motoneuron morphology among motoneurons innervating the medial and lateral regions of the PI muscle. However, the number of 5-HT terminals and the 5-HT terminal density (normalized for surface area) were greater in motoneurons innervating the medial region of the PI muscle compared with the lateral region. These results suggest that differences in distribution of 5-HT input may contribute to regional differences in PI muscle activation during inspiration and that differences in PI motoneuron recruitment do not relate to size.",
keywords = "Biogenic amines, Confocal microscopy, Immunohistochemistry, Neuromodulation, Respiratory muscles, Spinal cord",
author = "Zhan, {Wen Zhi} and Mantilla, {Carlos B.} and Phillip Zhan and Asaf Bitton and Prakash, {Y. S.} and {De Troyer}, Andre and Sieck, {Gary C.}",
doi = "10.1152/jappl.2000.88.5.1581",
journal = "Journal of Applied Physiology",
T1 - Regional differences in serotonergic input to canine parasternal intercostal motoneurons
AU - Zhan, Wen Zhi
AU - Mantilla, Carlos B.
AU - Zhan, Phillip
AU - Bitton, Asaf
AU - Prakash, Y. S.
AU - De Troyer, Andre
AU - Sieck, Gary C.
N2 - There is a mediolateral gradient in activation of the parasternal intercostal (PI) muscle during inspiration. In the present study, we tested the hypotheses that serotonergic [5-hydroxytryptamine (5-HT)] input from descending central drive and/or intrinsic size-related properties of PI motoneurons leads to the differential activation of PI muscles. In dogs, PI motoneurons innervating the medial and lateral regions of the PI muscles at the T3-T5 interspaces were retrogradely labeled by intramuscular injection of cholera toxin B subunit. After a 10-day survival period, PI motoneurons and 5-HT terminals were visualized by using immunohistochemistry and confocal imaging. There were no differences in motoneuron morphology among motoneurons innervating the medial and lateral regions of the PI muscle. However, the number of 5-HT terminals and the 5-HT terminal density (normalized for surface area) were greater in motoneurons innervating the medial region of the PI muscle compared with the lateral region. These results suggest that differences in distribution of 5-HT input may contribute to regional differences in PI muscle activation during inspiration and that differences in PI motoneuron recruitment do not relate to size.
AB - There is a mediolateral gradient in activation of the parasternal intercostal (PI) muscle during inspiration. In the present study, we tested the hypotheses that serotonergic [5-hydroxytryptamine (5-HT)] input from descending central drive and/or intrinsic size-related properties of PI motoneurons leads to the differential activation of PI muscles. In dogs, PI motoneurons innervating the medial and lateral regions of the PI muscles at the T3-T5 interspaces were retrogradely labeled by intramuscular injection of cholera toxin B subunit. After a 10-day survival period, PI motoneurons and 5-HT terminals were visualized by using immunohistochemistry and confocal imaging. There were no differences in motoneuron morphology among motoneurons innervating the medial and lateral regions of the PI muscle. However, the number of 5-HT terminals and the 5-HT terminal density (normalized for surface area) were greater in motoneurons innervating the medial region of the PI muscle compared with the lateral region. These results suggest that differences in distribution of 5-HT input may contribute to regional differences in PI muscle activation during inspiration and that differences in PI motoneuron recruitment do not relate to size.
KW - Biogenic amines
KW - Confocal microscopy
KW - Immunohistochemistry
KW - Respiratory muscles
U2 - 10.1152/jappl.2000.88.5.1581
DO - 10.1152/jappl.2000.88.5.1581
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology