Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity
Yanlong Jiang, Qingke Kong, Kenneth L. Roland, Roy Curtiss
Immunotherapy, Vaccines and Virotherapy, Center for (CIVV)
Vesicle shedding from bacteria is a universal process in most Gram-negative bacteria and a few Gram-positive bacteria. In this report, we isolate extracellular membrane vesicles (MVs) from the supernatants of Gram-positive pathogen Clostridium perfringens (C. perfringens). We demonstrated vesicle production in a variety of virulent and nonvirulent type A strains. MVs did not contain alpha-toxin and NetB toxin demonstrated by negative reaction to specific antibody and absence of specific proteins identified by LC-MS/MS. C. perfringens MVs contained DNA components such as 16S ribosomal RNA gene (16S rRNA), alpha-toxin gene (plc) and the perfringolysin O gene (pfoA) demonstrated by PCR. We also identified a total of 431 proteins in vesicles by 1-D gel separation and LC-MS/MS analysis. In vitro studies demonstrated that vesicles could be internalized into murine macrophage RAW264.7 cells without direct cytotoxicity effects, causing release of inflammation cytokines including granulocyte colony stimulating factor (G-CSF), tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1), which could also be detected in mice injected with MVs through intraperitoneal (i.p.) route. Mice immunized with C. perfringens MVs produced high titer IgG, especially IgG1, antibodies against C. perfringens membrane proteins. However, this kind of antibody could not provide protection in mice following challenge, though it could slightly postpone the time of death. Our results indicate that release of MVs from C. perfringens could provide a previously unknown mechanism to induce release of inflammatory cytokines, especially TNF-α, these findings may contribute to a better understanding of the pathogenesis of C. perfringens infection.
International Journal of Medical Microbiology
https://doi.org/10.1016/j.ijmm.2014.02.006
C. perfringens
Membrane vesicles
10.1016/j.ijmm.2014.02.006
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Clostridium perfringens Medicine & Life Sciences 100%
Adaptive Immunity Medicine & Life Sciences 75%
Innate Immunity Medicine & Life Sciences 68%
Membranes Medicine & Life Sciences 44%
Clostridium perfringens theta-toxin Medicine & Life Sciences 22%
Immunoglobulin G Medicine & Life Sciences 18%
Tumor Necrosis Factor-alpha Medicine & Life Sciences 17%
Jiang, Y., Kong, Q., Roland, K. L., & Curtiss, R. (2014). Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity. International Journal of Medical Microbiology, 304(3-4), 431-443. https://doi.org/10.1016/j.ijmm.2014.02.006
Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity. / Jiang, Yanlong; Kong, Qingke; Roland, Kenneth L. et al.
In: International Journal of Medical Microbiology, Vol. 304, No. 3-4, 05.2014, p. 431-443.
Jiang, Y, Kong, Q, Roland, KL & Curtiss, R 2014, 'Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity', International Journal of Medical Microbiology, vol. 304, no. 3-4, pp. 431-443. https://doi.org/10.1016/j.ijmm.2014.02.006
Jiang Y, Kong Q, Roland KL, Curtiss R. Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity. International Journal of Medical Microbiology. 2014 May;304(3-4):431-443. doi: 10.1016/j.ijmm.2014.02.006
Jiang, Yanlong ; Kong, Qingke ; Roland, Kenneth L. et al. / Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity. In: International Journal of Medical Microbiology. 2014 ; Vol. 304, No. 3-4. pp. 431-443.
@article{8b2d9ea10a80494d9e058d852eb1552c,
title = "Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity",
abstract = "Vesicle shedding from bacteria is a universal process in most Gram-negative bacteria and a few Gram-positive bacteria. In this report, we isolate extracellular membrane vesicles (MVs) from the supernatants of Gram-positive pathogen Clostridium perfringens (C. perfringens). We demonstrated vesicle production in a variety of virulent and nonvirulent type A strains. MVs did not contain alpha-toxin and NetB toxin demonstrated by negative reaction to specific antibody and absence of specific proteins identified by LC-MS/MS. C. perfringens MVs contained DNA components such as 16S ribosomal RNA gene (16S rRNA), alpha-toxin gene (plc) and the perfringolysin O gene (pfoA) demonstrated by PCR. We also identified a total of 431 proteins in vesicles by 1-D gel separation and LC-MS/MS analysis. In vitro studies demonstrated that vesicles could be internalized into murine macrophage RAW264.7 cells without direct cytotoxicity effects, causing release of inflammation cytokines including granulocyte colony stimulating factor (G-CSF), tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1), which could also be detected in mice injected with MVs through intraperitoneal (i.p.) route. Mice immunized with C. perfringens MVs produced high titer IgG, especially IgG1, antibodies against C. perfringens membrane proteins. However, this kind of antibody could not provide protection in mice following challenge, though it could slightly postpone the time of death. Our results indicate that release of MVs from C. perfringens could provide a previously unknown mechanism to induce release of inflammatory cytokines, especially TNF-α, these findings may contribute to a better understanding of the pathogenesis of C. perfringens infection.",
keywords = "C. perfringens, Immunity, Membrane vesicles",
author = "Yanlong Jiang and Qingke Kong and Roland, {Kenneth L.} and Roy Curtiss",
note = "Funding Information: The work was supported by grant R01 AI60557 from the National Institutes of Health. We thank Dr. Gregory R. Siragusa (Danisco USA Inc, WI, USA) , Dr. John F. Prescott (University of Guelph, Ontario, Canada) and Dr. Glenn Songer (Iowa State University, Ames, Iowa) for providing C. perfringens strains. We also thank Jacquelyn Kilbourne for assistance with antibody production. Mass spectrometry and proteomics data were acquired by the Arizona Proteomics Consortium supported by NIEHS grant ES06694 to the SWEHSC, NIH/NCI grant CA023074 to the AZCC and by the BIO5 Institute of the University of Arizona . The Thermo Fisher LTQ Orbitrap Velos mass spectrometer was provided by grant 1S10 RR028868-01 from NIH/NCRR . ",
doi = "10.1016/j.ijmm.2014.02.006",
journal = "International Journal of Medical Microbiology",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
T1 - Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity
AU - Jiang, Yanlong
AU - Kong, Qingke
AU - Roland, Kenneth L.
AU - Curtiss, Roy
N1 - Funding Information: The work was supported by grant R01 AI60557 from the National Institutes of Health. We thank Dr. Gregory R. Siragusa (Danisco USA Inc, WI, USA) , Dr. John F. Prescott (University of Guelph, Ontario, Canada) and Dr. Glenn Songer (Iowa State University, Ames, Iowa) for providing C. perfringens strains. We also thank Jacquelyn Kilbourne for assistance with antibody production. Mass spectrometry and proteomics data were acquired by the Arizona Proteomics Consortium supported by NIEHS grant ES06694 to the SWEHSC, NIH/NCI grant CA023074 to the AZCC and by the BIO5 Institute of the University of Arizona . The Thermo Fisher LTQ Orbitrap Velos mass spectrometer was provided by grant 1S10 RR028868-01 from NIH/NCRR .
N2 - Vesicle shedding from bacteria is a universal process in most Gram-negative bacteria and a few Gram-positive bacteria. In this report, we isolate extracellular membrane vesicles (MVs) from the supernatants of Gram-positive pathogen Clostridium perfringens (C. perfringens). We demonstrated vesicle production in a variety of virulent and nonvirulent type A strains. MVs did not contain alpha-toxin and NetB toxin demonstrated by negative reaction to specific antibody and absence of specific proteins identified by LC-MS/MS. C. perfringens MVs contained DNA components such as 16S ribosomal RNA gene (16S rRNA), alpha-toxin gene (plc) and the perfringolysin O gene (pfoA) demonstrated by PCR. We also identified a total of 431 proteins in vesicles by 1-D gel separation and LC-MS/MS analysis. In vitro studies demonstrated that vesicles could be internalized into murine macrophage RAW264.7 cells without direct cytotoxicity effects, causing release of inflammation cytokines including granulocyte colony stimulating factor (G-CSF), tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1), which could also be detected in mice injected with MVs through intraperitoneal (i.p.) route. Mice immunized with C. perfringens MVs produced high titer IgG, especially IgG1, antibodies against C. perfringens membrane proteins. However, this kind of antibody could not provide protection in mice following challenge, though it could slightly postpone the time of death. Our results indicate that release of MVs from C. perfringens could provide a previously unknown mechanism to induce release of inflammatory cytokines, especially TNF-α, these findings may contribute to a better understanding of the pathogenesis of C. perfringens infection.
AB - Vesicle shedding from bacteria is a universal process in most Gram-negative bacteria and a few Gram-positive bacteria. In this report, we isolate extracellular membrane vesicles (MVs) from the supernatants of Gram-positive pathogen Clostridium perfringens (C. perfringens). We demonstrated vesicle production in a variety of virulent and nonvirulent type A strains. MVs did not contain alpha-toxin and NetB toxin demonstrated by negative reaction to specific antibody and absence of specific proteins identified by LC-MS/MS. C. perfringens MVs contained DNA components such as 16S ribosomal RNA gene (16S rRNA), alpha-toxin gene (plc) and the perfringolysin O gene (pfoA) demonstrated by PCR. We also identified a total of 431 proteins in vesicles by 1-D gel separation and LC-MS/MS analysis. In vitro studies demonstrated that vesicles could be internalized into murine macrophage RAW264.7 cells without direct cytotoxicity effects, causing release of inflammation cytokines including granulocyte colony stimulating factor (G-CSF), tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1), which could also be detected in mice injected with MVs through intraperitoneal (i.p.) route. Mice immunized with C. perfringens MVs produced high titer IgG, especially IgG1, antibodies against C. perfringens membrane proteins. However, this kind of antibody could not provide protection in mice following challenge, though it could slightly postpone the time of death. Our results indicate that release of MVs from C. perfringens could provide a previously unknown mechanism to induce release of inflammatory cytokines, especially TNF-α, these findings may contribute to a better understanding of the pathogenesis of C. perfringens infection.
KW - C. perfringens
KW - Immunity
KW - Membrane vesicles
U2 - 10.1016/j.ijmm.2014.02.006
DO - 10.1016/j.ijmm.2014.02.006
JO - International Journal of Medical Microbiology
JF - International Journal of Medical Microbiology