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Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)†
Kuangwen Hsieh,‡a Peter L. Mage,‡b Andrew T. Csordas,c Michael Eisensteinc and H. Tom Soh*abc
* Corresponding authors
a Department of Mechanical Engineering, University of California, Santa Barbara, CA 93106, USA
E-mail: tsoh@engr.ucsb.edu
Tel: +1 805 8937985
b Materials Department, University of California, Santa Barbara, CA 93106, USA
c Institute for Collaborative Biotechnologies, University of California, Santa Barbara, CA 93106, USA
We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples.
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https://doi.org/10.1039/C4CC00540F
Chem. Commun., 2014,50, 3747-3749
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Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)
K. Hsieh, P. L. Mage, A. T. Csordas, M. Eisenstein and H. Tom Soh, Chem. Commun., 2014, 50, 3747 DOI: 10.1039/C4CC00540F
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Kuangwen Hsieh
Peter L. Mage
Andrew T. Csordas
Michael Eisenstein
H. Tom Soh
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