Factors affecting use of fluorescent agents in identification of resin-based polymers
Paulo H.P. D'Alpino, José Carlos Pereira, Nádia R. Svizero, Frederick Rueggeberg, David Henry Pashley
Purpose: The present study is a compilation of methodologies developed in order to evaluate the effects of addition of a fluorescent agent, rhodamine B, to resin-based materials. The intent of the work was to develop a systematic methodology that accounts for variables not shown to be of concern in past testing, but may significantly affect interpretation of the resulting images and material properties. Materials and Methods: Different methodologies were specifically developed to evaluate factors affecting the use of the fluorescent agent rhodamine B in the identification of resin-based materials. The influence of dye concentration was evaluated with respect to adhesive polymerization reaction kinetics and bond strength of the resin into which the dye is added. The pH of the dye-solvent solution was also considered. Additionally, the mass and percentage of rhodamine B leached into different media over time was determined. Results: It was demonstrated that a specific dye must be used with caution, and the concentration of a fluorescent agent is important with respect to both the conversion and bond strength of the resin to which the dye is added. An alcohol-based dye solution also produced an extremely high amount of dye leaching from polymerized specimens. The adhesive pH was not altered. Conclusion: The standardized methodology developed here to analyze restoration interfaces using a fluorescent dye can lessen the impact of variables when interpreting results.
Published - Nov 2 2006
Dental adhesives
Fingerprint Dive into the research topics of 'Factors affecting use of fluorescent agents in identification of resin-based polymers'. Together they form a unique fingerprint.
Fluorescent Dyes Medicine & Life Sciences
Polymers Medicine & Life Sciences
Coloring Agents Medicine & Life Sciences
rhodamine B Medicine & Life Sciences
Adhesives Medicine & Life Sciences
Alcohols Medicine & Life Sciences
Kinetics Medicine & Life Sciences
D'Alpino, P. H. P., Pereira, J. C., Svizero, N. R., Rueggeberg, F., & Pashley, D. H. (2006). Factors affecting use of fluorescent agents in identification of resin-based polymers. Journal of Adhesive Dentistry, 8(5), 285-292.
Factors affecting use of fluorescent agents in identification of resin-based polymers. / D'Alpino, Paulo H.P.; Pereira, José Carlos; Svizero, Nádia R.; Rueggeberg, Frederick; Pashley, David Henry.
In: Journal of Adhesive Dentistry, Vol. 8, No. 5, 02.11.2006, p. 285-292.
D'Alpino, PHP, Pereira, JC, Svizero, NR, Rueggeberg, F & Pashley, DH 2006, 'Factors affecting use of fluorescent agents in identification of resin-based polymers', Journal of Adhesive Dentistry, vol. 8, no. 5, pp. 285-292.
D'Alpino PHP, Pereira JC, Svizero NR, Rueggeberg F, Pashley DH. Factors affecting use of fluorescent agents in identification of resin-based polymers. Journal of Adhesive Dentistry. 2006 Nov 2;8(5):285-292.
D'Alpino, Paulo H.P. ; Pereira, José Carlos ; Svizero, Nádia R. ; Rueggeberg, Frederick ; Pashley, David Henry. / Factors affecting use of fluorescent agents in identification of resin-based polymers. In: Journal of Adhesive Dentistry. 2006 ; Vol. 8, No. 5. pp. 285-292.
@article{741d7a86f71741528af1284099b15d40,
title = "Factors affecting use of fluorescent agents in identification of resin-based polymers",
abstract = "Purpose: The present study is a compilation of methodologies developed in order to evaluate the effects of addition of a fluorescent agent, rhodamine B, to resin-based materials. The intent of the work was to develop a systematic methodology that accounts for variables not shown to be of concern in past testing, but may significantly affect interpretation of the resulting images and material properties. Materials and Methods: Different methodologies were specifically developed to evaluate factors affecting the use of the fluorescent agent rhodamine B in the identification of resin-based materials. The influence of dye concentration was evaluated with respect to adhesive polymerization reaction kinetics and bond strength of the resin into which the dye is added. The pH of the dye-solvent solution was also considered. Additionally, the mass and percentage of rhodamine B leached into different media over time was determined. Results: It was demonstrated that a specific dye must be used with caution, and the concentration of a fluorescent agent is important with respect to both the conversion and bond strength of the resin to which the dye is added. An alcohol-based dye solution also produced an extremely high amount of dye leaching from polymerized specimens. The adhesive pH was not altered. Conclusion: The standardized methodology developed here to analyze restoration interfaces using a fluorescent dye can lessen the impact of variables when interpreting results.",
keywords = "Dental adhesives, Fluorescence microscopy, Fluorescent dyes",
author = "D'Alpino, {Paulo H.P.} and Pereira, {Jos{\'e} Carlos} and Svizero, {N{\'a}dia R.} and Frederick Rueggeberg and Pashley, {David Henry}",
journal = "The journal of adhesive dentistry",
publisher = "Quintessence Publishing Company",
T1 - Factors affecting use of fluorescent agents in identification of resin-based polymers
AU - D'Alpino, Paulo H.P.
AU - Pereira, José Carlos
AU - Svizero, Nádia R.
AU - Rueggeberg, Frederick
AU - Pashley, David Henry
N2 - Purpose: The present study is a compilation of methodologies developed in order to evaluate the effects of addition of a fluorescent agent, rhodamine B, to resin-based materials. The intent of the work was to develop a systematic methodology that accounts for variables not shown to be of concern in past testing, but may significantly affect interpretation of the resulting images and material properties. Materials and Methods: Different methodologies were specifically developed to evaluate factors affecting the use of the fluorescent agent rhodamine B in the identification of resin-based materials. The influence of dye concentration was evaluated with respect to adhesive polymerization reaction kinetics and bond strength of the resin into which the dye is added. The pH of the dye-solvent solution was also considered. Additionally, the mass and percentage of rhodamine B leached into different media over time was determined. Results: It was demonstrated that a specific dye must be used with caution, and the concentration of a fluorescent agent is important with respect to both the conversion and bond strength of the resin to which the dye is added. An alcohol-based dye solution also produced an extremely high amount of dye leaching from polymerized specimens. The adhesive pH was not altered. Conclusion: The standardized methodology developed here to analyze restoration interfaces using a fluorescent dye can lessen the impact of variables when interpreting results.
AB - Purpose: The present study is a compilation of methodologies developed in order to evaluate the effects of addition of a fluorescent agent, rhodamine B, to resin-based materials. The intent of the work was to develop a systematic methodology that accounts for variables not shown to be of concern in past testing, but may significantly affect interpretation of the resulting images and material properties. Materials and Methods: Different methodologies were specifically developed to evaluate factors affecting the use of the fluorescent agent rhodamine B in the identification of resin-based materials. The influence of dye concentration was evaluated with respect to adhesive polymerization reaction kinetics and bond strength of the resin into which the dye is added. The pH of the dye-solvent solution was also considered. Additionally, the mass and percentage of rhodamine B leached into different media over time was determined. Results: It was demonstrated that a specific dye must be used with caution, and the concentration of a fluorescent agent is important with respect to both the conversion and bond strength of the resin to which the dye is added. An alcohol-based dye solution also produced an extremely high amount of dye leaching from polymerized specimens. The adhesive pH was not altered. Conclusion: The standardized methodology developed here to analyze restoration interfaces using a fluorescent dye can lessen the impact of variables when interpreting results.
KW - Dental adhesives
KW - Fluorescence microscopy
JO - The journal of adhesive dentistry
JF - The journal of adhesive dentistry