Increase in cocaine- and amphetamine-regulated transcript (CART) in specific areas of the mouse brain by acute caffeine administration
Jin Hee Cho, Yun Ha Cho, Hyo Young Kim, Seung Ha Cha, Hyun Ryu, Wooyoung Jang, Kyung-Ho Shin
Caffeine produces a variety of behavioral effects including increased alertness, reduced food intake, anxiogenic effects, and dependence upon repeated exposure. Although many of the effects of caffeine are mediated by its ability to block adenosine receptors, it is possible that other neural substrates, such as cocaine- and amphetamine-regulated transcript (CART), may be involved in the effects of caffeine. Indeed, a recent study demonstrated that repeated caffeine administration increases CART in the mouse striatum. However, it is not clear whether acute caffeine administration alters CART in other areas of the brain. To explore this possibility, we investigated the dose- and time-dependent changes in CART immunoreactivity (CART-IR) after a single dose of caffeine in mice. We found that a high dose of caffeine (100 mg/kg) significantly increased CART-IR 2 h after administration in the nucleus accumbens shell (AcbSh), dorsal bed nucleus of the stria terminalis (dBNST), central nucleus of the amygdala (CeA), paraventricular hypothalamic nucleus (PVN), arcuate hypothalamic nucleus (Arc), and locus coeruleus (LC), and returned to control levels after 8 h. But this increase was not observed in other brain areas. In addition, caffeine administration at doses of 25 and 50 mg/kg appears to produce dose-dependent increases in CART-IR in these brain areas; however, the magnitude of increase in CART-IR observed at a dose of 50 mg/kg was similar or greater than that observed at a dose of 100 mg/kg. This result suggests that CART-IR in AcbSh, dBNST, CeA, PVN, Arc, and LC is selectively affected by caffeine administration.
Neuropeptides
https://doi.org/10.1016/j.npep.2015.03.004
Published - 2014 Oct 22
Septal Nuclei
Paraventricular Hypothalamic Nucleus
Arcuate Nucleus of Hypothalamus
Purinergic P1 Receptors
Nucleus Accumbens
Arcuate hypothalamic nucleus
Bed nucleus of the stria terminalis
Central nucleus of the amygdala
Cocaine- and amphetamine-regulated transcript
Cho, J. H., Cho, Y. H., Kim, H. Y., Cha, S. H., Ryu, H., Jang, W., & Shin, K-H. (2014). Increase in cocaine- and amphetamine-regulated transcript (CART) in specific areas of the mouse brain by acute caffeine administration. Neuropeptides, 50, 1-7. https://doi.org/10.1016/j.npep.2015.03.004
Increase in cocaine- and amphetamine-regulated transcript (CART) in specific areas of the mouse brain by acute caffeine administration. / Cho, Jin Hee; Cho, Yun Ha; Kim, Hyo Young; Cha, Seung Ha; Ryu, Hyun; Jang, Wooyoung; Shin, Kyung-Ho.
In: Neuropeptides, Vol. 50, 22.10.2014, p. 1-7.
Cho, JH, Cho, YH, Kim, HY, Cha, SH, Ryu, H, Jang, W & Shin, K-H 2014, 'Increase in cocaine- and amphetamine-regulated transcript (CART) in specific areas of the mouse brain by acute caffeine administration', Neuropeptides, vol. 50, pp. 1-7. https://doi.org/10.1016/j.npep.2015.03.004
Cho JH, Cho YH, Kim HY, Cha SH, Ryu H, Jang W et al. Increase in cocaine- and amphetamine-regulated transcript (CART) in specific areas of the mouse brain by acute caffeine administration. Neuropeptides. 2014 Oct 22;50:1-7. https://doi.org/10.1016/j.npep.2015.03.004
Cho, Jin Hee ; Cho, Yun Ha ; Kim, Hyo Young ; Cha, Seung Ha ; Ryu, Hyun ; Jang, Wooyoung ; Shin, Kyung-Ho. / Increase in cocaine- and amphetamine-regulated transcript (CART) in specific areas of the mouse brain by acute caffeine administration. In: Neuropeptides. 2014 ; Vol. 50. pp. 1-7.
@article{eb7cf372b145413c847cf73765c85226,
title = "Increase in cocaine- and amphetamine-regulated transcript (CART) in specific areas of the mouse brain by acute caffeine administration",
abstract = "Caffeine produces a variety of behavioral effects including increased alertness, reduced food intake, anxiogenic effects, and dependence upon repeated exposure. Although many of the effects of caffeine are mediated by its ability to block adenosine receptors, it is possible that other neural substrates, such as cocaine- and amphetamine-regulated transcript (CART), may be involved in the effects of caffeine. Indeed, a recent study demonstrated that repeated caffeine administration increases CART in the mouse striatum. However, it is not clear whether acute caffeine administration alters CART in other areas of the brain. To explore this possibility, we investigated the dose- and time-dependent changes in CART immunoreactivity (CART-IR) after a single dose of caffeine in mice. We found that a high dose of caffeine (100 mg/kg) significantly increased CART-IR 2 h after administration in the nucleus accumbens shell (AcbSh), dorsal bed nucleus of the stria terminalis (dBNST), central nucleus of the amygdala (CeA), paraventricular hypothalamic nucleus (PVN), arcuate hypothalamic nucleus (Arc), and locus coeruleus (LC), and returned to control levels after 8 h. But this increase was not observed in other brain areas. In addition, caffeine administration at doses of 25 and 50 mg/kg appears to produce dose-dependent increases in CART-IR in these brain areas; however, the magnitude of increase in CART-IR observed at a dose of 50 mg/kg was similar or greater than that observed at a dose of 100 mg/kg. This result suggests that CART-IR in AcbSh, dBNST, CeA, PVN, Arc, and LC is selectively affected by caffeine administration.",
keywords = "Arcuate hypothalamic nucleus, Bed nucleus of the stria terminalis, Caffeine, Central nucleus of the amygdala, Cocaine- and amphetamine-regulated transcript, Locus coeruleus, Nucleus accumbens, Paraventricular hypothalamic nucleus",
author = "Cho, {Jin Hee} and Cho, {Yun Ha} and Kim, {Hyo Young} and Cha, {Seung Ha} and Hyun Ryu and Wooyoung Jang and Kyung-Ho Shin",
doi = "10.1016/j.npep.2015.03.004",
journal = "Neuropeptides",
publisher = "Churchill Livingstone",
T1 - Increase in cocaine- and amphetamine-regulated transcript (CART) in specific areas of the mouse brain by acute caffeine administration
AU - Cho, Jin Hee
AU - Cho, Yun Ha
AU - Kim, Hyo Young
AU - Cha, Seung Ha
AU - Ryu, Hyun
AU - Jang, Wooyoung
AU - Shin, Kyung-Ho
N2 - Caffeine produces a variety of behavioral effects including increased alertness, reduced food intake, anxiogenic effects, and dependence upon repeated exposure. Although many of the effects of caffeine are mediated by its ability to block adenosine receptors, it is possible that other neural substrates, such as cocaine- and amphetamine-regulated transcript (CART), may be involved in the effects of caffeine. Indeed, a recent study demonstrated that repeated caffeine administration increases CART in the mouse striatum. However, it is not clear whether acute caffeine administration alters CART in other areas of the brain. To explore this possibility, we investigated the dose- and time-dependent changes in CART immunoreactivity (CART-IR) after a single dose of caffeine in mice. We found that a high dose of caffeine (100 mg/kg) significantly increased CART-IR 2 h after administration in the nucleus accumbens shell (AcbSh), dorsal bed nucleus of the stria terminalis (dBNST), central nucleus of the amygdala (CeA), paraventricular hypothalamic nucleus (PVN), arcuate hypothalamic nucleus (Arc), and locus coeruleus (LC), and returned to control levels after 8 h. But this increase was not observed in other brain areas. In addition, caffeine administration at doses of 25 and 50 mg/kg appears to produce dose-dependent increases in CART-IR in these brain areas; however, the magnitude of increase in CART-IR observed at a dose of 50 mg/kg was similar or greater than that observed at a dose of 100 mg/kg. This result suggests that CART-IR in AcbSh, dBNST, CeA, PVN, Arc, and LC is selectively affected by caffeine administration.
AB - Caffeine produces a variety of behavioral effects including increased alertness, reduced food intake, anxiogenic effects, and dependence upon repeated exposure. Although many of the effects of caffeine are mediated by its ability to block adenosine receptors, it is possible that other neural substrates, such as cocaine- and amphetamine-regulated transcript (CART), may be involved in the effects of caffeine. Indeed, a recent study demonstrated that repeated caffeine administration increases CART in the mouse striatum. However, it is not clear whether acute caffeine administration alters CART in other areas of the brain. To explore this possibility, we investigated the dose- and time-dependent changes in CART immunoreactivity (CART-IR) after a single dose of caffeine in mice. We found that a high dose of caffeine (100 mg/kg) significantly increased CART-IR 2 h after administration in the nucleus accumbens shell (AcbSh), dorsal bed nucleus of the stria terminalis (dBNST), central nucleus of the amygdala (CeA), paraventricular hypothalamic nucleus (PVN), arcuate hypothalamic nucleus (Arc), and locus coeruleus (LC), and returned to control levels after 8 h. But this increase was not observed in other brain areas. In addition, caffeine administration at doses of 25 and 50 mg/kg appears to produce dose-dependent increases in CART-IR in these brain areas; however, the magnitude of increase in CART-IR observed at a dose of 50 mg/kg was similar or greater than that observed at a dose of 100 mg/kg. This result suggests that CART-IR in AcbSh, dBNST, CeA, PVN, Arc, and LC is selectively affected by caffeine administration.
KW - Arcuate hypothalamic nucleus
KW - Bed nucleus of the stria terminalis
KW - Caffeine
KW - Central nucleus of the amygdala
KW - Cocaine- and amphetamine-regulated transcript
KW - Locus coeruleus
KW - Nucleus accumbens
KW - Paraventricular hypothalamic nucleus
U2 - 10.1016/j.npep.2015.03.004
DO - 10.1016/j.npep.2015.03.004
JO - Neuropeptides
JF - Neuropeptides
10.1016/j.npep.2015.03.004