Global Stoichiometry: Hetero 3-mer - ABC
Unique nucleic acid chains: 6
3EQ3
Model of tRNA(Trp)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM
DOI: 10.2210/pdb3EQ3/pdb
Classification: RIBOSOMAL PROTEIN/RNA
Organism(s): Escherichia coli K-12, Escherichia coli (strain K12)
Deposition Author(s): Frank, J., Li, W., Agirrezabala, X.
Resolution: 9 Å
Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM.
Li, W., Agirrezabala, X., Lei, J., Bouakaz, L., Brunelle, J.L., Ortiz-Meoz, R.F., Green, R., Sanyal, S., Ehrenberg, M., Frank, J.
(2008) Embo J. 27: 3322-3331
DOI: 10.1038/emboj.2008.243
3EQ4, 3EP2
The accuracy of ribosomal translation is achieved by an initial selection and a proofreading step, mediated by EF-Tu, which forms a ternary complex with aminoacyl(aa)-tRNA. To study the binding modes of different aa-tRNAs, we compared cryo-EM maps of ...
The accuracy of ribosomal translation is achieved by an initial selection and a proofreading step, mediated by EF-Tu, which forms a ternary complex with aminoacyl(aa)-tRNA. To study the binding modes of different aa-tRNAs, we compared cryo-EM maps of the kirromycin-stalled ribosome bound with ternary complexes containing Phe-tRNA(Phe), Trp-tRNA(Trp), or Leu-tRNA(LeuI). The three maps suggest a common binding manner of cognate aa-tRNAs in their specific binding with both the ribosome and EF-Tu. All three aa-tRNAs have the same 'loaded spring' conformation with a kink and twist between the D-stem and anticodon stem. The three complexes are similarly integrated in an interaction network, extending from the anticodon loop through h44 and protein S12 to the EF-Tu-binding CCA end of aa-tRNA, proposed to signal cognate codon-anticodon interaction to the GTPase centre and tune the accuracy of aa-tRNA selection.
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Proteins 3
Nucleic Acids / Hybrid 6
Elongation factor Tu
393 Escherichia coli (strain K12) Mutation(s): 0
Gene Names: tufB
Find proteins for P0CE48 (Escherichia coli (strain K12))
Go to UniProtKB: P0CE48
Full Protein Feature View for P0CE48
30S ribosomal protein S12
Gene Names: rpsL (strA)
Find proteins for P0A7S3 (Escherichia coli (strain K12))
Go to UniProtKB: P0A7S3
Full Protein Feature View for P0A7S3
50S ribosomal protein L11
Gene Names: rplK (relC)
Find proteins for P0A7J7 (Escherichia coli (strain K12))
Go to UniProtKB: P0A7J7
Full Protein Feature View for P0A7J7
tRNA Y 74 Escherichia coli K-12
Fragment h18 of the 16S rRNA A 9 Escherichia coli K-12
Fragment h44 of the 16S rRNA C 11 Escherichia coli K-12
Fragment H43-44 of the 23S rRNA B 48 Escherichia coli K-12
Fragment H95 of the 23S rRNA D 28 Escherichia coli K-12
Fragment H69 of the 23S rRNA E 17 Escherichia coli K-12
Type: Data collection