Activation of Nippostrongylus brasiliensis infective larvae is regulated by a pathway distinct from the hookworm Ancylostoma caninum
Stanley Ching Cheng Huang, Denice Tsz Yau Chan, Danielle J. Smyth, Glyn Ball, Kleoniki Gounaris, Murray E. Selkirk
Cell Signalling and Immunology
Developmentally arrested infective larvae of strongylid nematodes are activated to resume growth by host-derived cues encountered during invasion of the mammalian host. Exposure of Nippostrongylus brasiliensis infective larvae to elevated temperature (37°C) is sufficient to activate signalling pathways which result in resumption of feeding and protein secretion. This occurs independently of exposure to serum or glutathione, in contrast to the hookworm Ancylostoma caninum, and is not initiated by chemical exsheathment. No qualitative differences in protein secretion were induced by host serum as visualised by two-dimensional SDS-PAGE, although exposure of larvae to an aqueous extract of rat skin did stimulate secretion of a small pre-synthesised bolus of proteins. Infective larvae began feeding after a lag period of 3-4. h at 37°C, reaching a maximum of 90% of the population feeding by 48. h. Neither a membrane permeant analogue of cyclic GMP nor muscarinic acetylcholine receptor agonists stimulated feeding at 20°C, and high concentrations of both compounds inhibited temperature-induced activation. LY294002, an inhibitor of phosphatidylinositol 3-kinase, Akt inhibitor IV, an inhibitor of Akt protein kinase, and ketoconazole, an inhibitor of cytochrome P450, all blocked resumption of feeding and protein secretion at 37°C. Serotonin increased the rate of feeding assessed by uptake of radiolabelled BSA, but could not initiate feeding independently of elevated temperature. Collectively, the data suggest that the early signalling events for larval activation in N. brasiliensis differ substantially from A. caninum, but that they may converge at pathways downstream of phosphatidylinositol 3-kinase involving steroid hormone synthesis.
International Journal for Parasitology
https://doi.org/10.1016/j.ijpara.2010.06.004
Nematode
Nippostrongylus brasiliensis
Transition to parasitism
10.1016/j.ijpara.2010.06.004
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Nippostrongylus Medicine & Life Sciences 100%
Ancylostoma Medicine & Life Sciences 99%
Nippostrongylus brasiliensis Agriculture & Biology 97%
Ancylostoma caninum Agriculture & Biology 96%
Ancylostomatoidea Medicine & Life Sciences 89%
hookworms Agriculture & Biology 88%
Larva Medicine & Life Sciences 68%
protein secretion Agriculture & Biology 57%
Huang, S. C. C., Chan, D. T. Y., Smyth, D. J., Ball, G., Gounaris, K., & Selkirk, M. E. (2010). Activation of Nippostrongylus brasiliensis infective larvae is regulated by a pathway distinct from the hookworm Ancylostoma caninum. International Journal for Parasitology, 40(14), 1619-1628. https://doi.org/10.1016/j.ijpara.2010.06.004
Huang, Stanley Ching Cheng ; Chan, Denice Tsz Yau ; Smyth, Danielle J. et al. / Activation of Nippostrongylus brasiliensis infective larvae is regulated by a pathway distinct from the hookworm Ancylostoma caninum. In: International Journal for Parasitology. 2010 ; Vol. 40, No. 14. pp. 1619-1628.
@article{90e5ed63ceab4224ab376d83f009f15b,
title = "Activation of Nippostrongylus brasiliensis infective larvae is regulated by a pathway distinct from the hookworm Ancylostoma caninum",
abstract = "Developmentally arrested infective larvae of strongylid nematodes are activated to resume growth by host-derived cues encountered during invasion of the mammalian host. Exposure of Nippostrongylus brasiliensis infective larvae to elevated temperature (37°C) is sufficient to activate signalling pathways which result in resumption of feeding and protein secretion. This occurs independently of exposure to serum or glutathione, in contrast to the hookworm Ancylostoma caninum, and is not initiated by chemical exsheathment. No qualitative differences in protein secretion were induced by host serum as visualised by two-dimensional SDS-PAGE, although exposure of larvae to an aqueous extract of rat skin did stimulate secretion of a small pre-synthesised bolus of proteins. Infective larvae began feeding after a lag period of 3-4. h at 37°C, reaching a maximum of 90% of the population feeding by 48. h. Neither a membrane permeant analogue of cyclic GMP nor muscarinic acetylcholine receptor agonists stimulated feeding at 20°C, and high concentrations of both compounds inhibited temperature-induced activation. LY294002, an inhibitor of phosphatidylinositol 3-kinase, Akt inhibitor IV, an inhibitor of Akt protein kinase, and ketoconazole, an inhibitor of cytochrome P450, all blocked resumption of feeding and protein secretion at 37°C. Serotonin increased the rate of feeding assessed by uptake of radiolabelled BSA, but could not initiate feeding independently of elevated temperature. Collectively, the data suggest that the early signalling events for larval activation in N. brasiliensis differ substantially from A. caninum, but that they may converge at pathways downstream of phosphatidylinositol 3-kinase involving steroid hormone synthesis.",
keywords = "Activation, Nematode, Nippostrongylus brasiliensis, Transition to parasitism",
author = "Huang, {Stanley Ching Cheng} and Chan, {Denice Tsz Yau} and Smyth, {Danielle J.} and Glyn Ball and Kleoniki Gounaris and Selkirk, {Murray E.}",
doi = "10.1016/j.ijpara.2010.06.004",
journal = "International Journal for Parasitology",
Huang, SCC, Chan, DTY, Smyth, DJ, Ball, G, Gounaris, K & Selkirk, ME 2010, 'Activation of Nippostrongylus brasiliensis infective larvae is regulated by a pathway distinct from the hookworm Ancylostoma caninum', International Journal for Parasitology, vol. 40, no. 14, pp. 1619-1628. https://doi.org/10.1016/j.ijpara.2010.06.004
Activation of Nippostrongylus brasiliensis infective larvae is regulated by a pathway distinct from the hookworm Ancylostoma caninum. / Huang, Stanley Ching Cheng; Chan, Denice Tsz Yau; Smyth, Danielle J. et al.
In: International Journal for Parasitology, Vol. 40, No. 14, 01.12.2010, p. 1619-1628.
T1 - Activation of Nippostrongylus brasiliensis infective larvae is regulated by a pathway distinct from the hookworm Ancylostoma caninum
AU - Huang, Stanley Ching Cheng
AU - Chan, Denice Tsz Yau
AU - Smyth, Danielle J.
AU - Ball, Glyn
AU - Gounaris, Kleoniki
AU - Selkirk, Murray E.
N2 - Developmentally arrested infective larvae of strongylid nematodes are activated to resume growth by host-derived cues encountered during invasion of the mammalian host. Exposure of Nippostrongylus brasiliensis infective larvae to elevated temperature (37°C) is sufficient to activate signalling pathways which result in resumption of feeding and protein secretion. This occurs independently of exposure to serum or glutathione, in contrast to the hookworm Ancylostoma caninum, and is not initiated by chemical exsheathment. No qualitative differences in protein secretion were induced by host serum as visualised by two-dimensional SDS-PAGE, although exposure of larvae to an aqueous extract of rat skin did stimulate secretion of a small pre-synthesised bolus of proteins. Infective larvae began feeding after a lag period of 3-4. h at 37°C, reaching a maximum of 90% of the population feeding by 48. h. Neither a membrane permeant analogue of cyclic GMP nor muscarinic acetylcholine receptor agonists stimulated feeding at 20°C, and high concentrations of both compounds inhibited temperature-induced activation. LY294002, an inhibitor of phosphatidylinositol 3-kinase, Akt inhibitor IV, an inhibitor of Akt protein kinase, and ketoconazole, an inhibitor of cytochrome P450, all blocked resumption of feeding and protein secretion at 37°C. Serotonin increased the rate of feeding assessed by uptake of radiolabelled BSA, but could not initiate feeding independently of elevated temperature. Collectively, the data suggest that the early signalling events for larval activation in N. brasiliensis differ substantially from A. caninum, but that they may converge at pathways downstream of phosphatidylinositol 3-kinase involving steroid hormone synthesis.
AB - Developmentally arrested infective larvae of strongylid nematodes are activated to resume growth by host-derived cues encountered during invasion of the mammalian host. Exposure of Nippostrongylus brasiliensis infective larvae to elevated temperature (37°C) is sufficient to activate signalling pathways which result in resumption of feeding and protein secretion. This occurs independently of exposure to serum or glutathione, in contrast to the hookworm Ancylostoma caninum, and is not initiated by chemical exsheathment. No qualitative differences in protein secretion were induced by host serum as visualised by two-dimensional SDS-PAGE, although exposure of larvae to an aqueous extract of rat skin did stimulate secretion of a small pre-synthesised bolus of proteins. Infective larvae began feeding after a lag period of 3-4. h at 37°C, reaching a maximum of 90% of the population feeding by 48. h. Neither a membrane permeant analogue of cyclic GMP nor muscarinic acetylcholine receptor agonists stimulated feeding at 20°C, and high concentrations of both compounds inhibited temperature-induced activation. LY294002, an inhibitor of phosphatidylinositol 3-kinase, Akt inhibitor IV, an inhibitor of Akt protein kinase, and ketoconazole, an inhibitor of cytochrome P450, all blocked resumption of feeding and protein secretion at 37°C. Serotonin increased the rate of feeding assessed by uptake of radiolabelled BSA, but could not initiate feeding independently of elevated temperature. Collectively, the data suggest that the early signalling events for larval activation in N. brasiliensis differ substantially from A. caninum, but that they may converge at pathways downstream of phosphatidylinositol 3-kinase involving steroid hormone synthesis.
KW - Activation
KW - Nematode
KW - Nippostrongylus brasiliensis
KW - Transition to parasitism
U2 - 10.1016/j.ijpara.2010.06.004
DO - 10.1016/j.ijpara.2010.06.004
JO - International Journal for Parasitology
JF - International Journal for Parasitology
Huang SCC, Chan DTY, Smyth DJ, Ball G, Gounaris K, Selkirk ME. Activation of Nippostrongylus brasiliensis infective larvae is regulated by a pathway distinct from the hookworm Ancylostoma caninum. International Journal for Parasitology. 2010 Dec 1;40(14):1619-1628. doi: 10.1016/j.ijpara.2010.06.004