Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction
Wilson C. Fok, Carolina Livi, Alex Bokov, Zhen Yu, Yidong Chen, Arlan Richardson, Viviana I. Pérez
Epidemiology & Biostatistics
Barshop Institute
Cell Systems & Anatomy
Rapamycin, a drug that has been shown to increase lifespan in mice, inhibits the target of rapamycin (TOR) pathway, a major pathway that regulates cell growth and energy status. It has been hypothesized that rapamycin and dietary restriction (DR) extend lifespan through similar mechanisms/pathways. Using microarray analysis, we compared the transcriptome of white adipose tissue from mice fed rapamycin or DR-diet for 6 months. Multidimensional scaling and heatmap analyses showed that rapamycin had essentially no effect on the transcriptome as compared to DR. For example, only six transcripts were significantly altered by rapamycin while mice fed DR showed a significant change in over 1000 transcripts. Using ingenuity pathway analysis, we found that stearate biosynthesis and circadian rhythm signaling were significantly changed by DR. Our findings showing that DR, but not rapamycin, has an effect on the transcriptome of the adipose tissue, suggesting that these two manipulations increase lifespan through different mechanisms/pathways.
Mechanisms of Ageing and Development
https://doi.org/10.1016/j.mad.2014.07.004
Adipose
10.1016/j.mad.2014.07.004
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White Adipose Tissue Medicine & Life Sciences 100%
Sirolimus Medicine & Life Sciences 87%
Transcriptome Medicine & Life Sciences 75%
Stearates Medicine & Life Sciences 18%
Circadian Rhythm Medicine & Life Sciences 12%
Microarray Analysis Medicine & Life Sciences 12%
Adipose Tissue Medicine & Life Sciences 9%
Fok, W. C., Livi, C., Bokov, A., Yu, Z., Chen, Y., Richardson, A., & Pérez, V. I. (2014). Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction. Mechanisms of Ageing and Development, 140(1), 23-29. https://doi.org/10.1016/j.mad.2014.07.004
Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction. / Fok, Wilson C.; Livi, Carolina; Bokov, Alex; Yu, Zhen; Chen, Yidong; Richardson, Arlan; Pérez, Viviana I.
In: Mechanisms of Ageing and Development, Vol. 140, No. 1, 09.2014, p. 23-29.
Fok, WC, Livi, C, Bokov, A, Yu, Z, Chen, Y, Richardson, A & Pérez, VI 2014, 'Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction', Mechanisms of Ageing and Development, vol. 140, no. 1, pp. 23-29. https://doi.org/10.1016/j.mad.2014.07.004
Fok WC, Livi C, Bokov A, Yu Z, Chen Y, Richardson A et al. Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction. Mechanisms of Ageing and Development. 2014 Sep;140(1):23-29. https://doi.org/10.1016/j.mad.2014.07.004
Fok, Wilson C. ; Livi, Carolina ; Bokov, Alex ; Yu, Zhen ; Chen, Yidong ; Richardson, Arlan ; Pérez, Viviana I. / Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction. In: Mechanisms of Ageing and Development. 2014 ; Vol. 140, No. 1. pp. 23-29.
@article{cd7c14bace2b4094a59dd2a7e5696373,
title = "Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction",
abstract = "Rapamycin, a drug that has been shown to increase lifespan in mice, inhibits the target of rapamycin (TOR) pathway, a major pathway that regulates cell growth and energy status. It has been hypothesized that rapamycin and dietary restriction (DR) extend lifespan through similar mechanisms/pathways. Using microarray analysis, we compared the transcriptome of white adipose tissue from mice fed rapamycin or DR-diet for 6 months. Multidimensional scaling and heatmap analyses showed that rapamycin had essentially no effect on the transcriptome as compared to DR. For example, only six transcripts were significantly altered by rapamycin while mice fed DR showed a significant change in over 1000 transcripts. Using ingenuity pathway analysis, we found that stearate biosynthesis and circadian rhythm signaling were significantly changed by DR. Our findings showing that DR, but not rapamycin, has an effect on the transcriptome of the adipose tissue, suggesting that these two manipulations increase lifespan through different mechanisms/pathways.",
keywords = "Adipose, Dietary restriction, Fat, Microarrays, Rapamycin, Transcriptome",
author = "Fok, {Wilson C.} and Carolina Livi and Alex Bokov and Zhen Yu and Yidong Chen and Arlan Richardson and P{\'e}rez, {Viviana I.}",
note = "Funding Information: We would like to thank UTHSCSA LAR for mouse husbandry and the Genomics Core Shared Resource, which is supported by UTHSCSA, NIH-NCI P30CA054174 (CTRC of UTHSCSA) for microarray hybridization and scanning, and Sherry Dodds for help in RNA isolation. Funding Information: Financial support was provided by The San Antonio Nathan Shock Aging Center ( 1p30-AG-13319 , AR), National Institutes of Health (NIH) RC2 Grand Opportunity grant ( AG036613 , AR), NIH T32 Training Grant ( AG021890 , WF), the Ellison Medical Foundation (VP), and the Intramural Research Program of the NIH, National Institute on Aging , and VA Merit grant ( 101BX000547 , AR) from the Department of Veteran Affairs . Computation Support was provided by the Computational System Biology Core funded by the National Institute on Minority Health and Health Disparities ( G12MD007591 ) from the NIH . ",
doi = "10.1016/j.mad.2014.07.004",
journal = "Mechanisms of Ageing and Development",
T1 - Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction
AU - Fok, Wilson C.
AU - Livi, Carolina
AU - Bokov, Alex
AU - Yu, Zhen
AU - Chen, Yidong
AU - Richardson, Arlan
AU - Pérez, Viviana I.
N1 - Funding Information: We would like to thank UTHSCSA LAR for mouse husbandry and the Genomics Core Shared Resource, which is supported by UTHSCSA, NIH-NCI P30CA054174 (CTRC of UTHSCSA) for microarray hybridization and scanning, and Sherry Dodds for help in RNA isolation. Funding Information: Financial support was provided by The San Antonio Nathan Shock Aging Center ( 1p30-AG-13319 , AR), National Institutes of Health (NIH) RC2 Grand Opportunity grant ( AG036613 , AR), NIH T32 Training Grant ( AG021890 , WF), the Ellison Medical Foundation (VP), and the Intramural Research Program of the NIH, National Institute on Aging , and VA Merit grant ( 101BX000547 , AR) from the Department of Veteran Affairs . Computation Support was provided by the Computational System Biology Core funded by the National Institute on Minority Health and Health Disparities ( G12MD007591 ) from the NIH .
N2 - Rapamycin, a drug that has been shown to increase lifespan in mice, inhibits the target of rapamycin (TOR) pathway, a major pathway that regulates cell growth and energy status. It has been hypothesized that rapamycin and dietary restriction (DR) extend lifespan through similar mechanisms/pathways. Using microarray analysis, we compared the transcriptome of white adipose tissue from mice fed rapamycin or DR-diet for 6 months. Multidimensional scaling and heatmap analyses showed that rapamycin had essentially no effect on the transcriptome as compared to DR. For example, only six transcripts were significantly altered by rapamycin while mice fed DR showed a significant change in over 1000 transcripts. Using ingenuity pathway analysis, we found that stearate biosynthesis and circadian rhythm signaling were significantly changed by DR. Our findings showing that DR, but not rapamycin, has an effect on the transcriptome of the adipose tissue, suggesting that these two manipulations increase lifespan through different mechanisms/pathways.
AB - Rapamycin, a drug that has been shown to increase lifespan in mice, inhibits the target of rapamycin (TOR) pathway, a major pathway that regulates cell growth and energy status. It has been hypothesized that rapamycin and dietary restriction (DR) extend lifespan through similar mechanisms/pathways. Using microarray analysis, we compared the transcriptome of white adipose tissue from mice fed rapamycin or DR-diet for 6 months. Multidimensional scaling and heatmap analyses showed that rapamycin had essentially no effect on the transcriptome as compared to DR. For example, only six transcripts were significantly altered by rapamycin while mice fed DR showed a significant change in over 1000 transcripts. Using ingenuity pathway analysis, we found that stearate biosynthesis and circadian rhythm signaling were significantly changed by DR. Our findings showing that DR, but not rapamycin, has an effect on the transcriptome of the adipose tissue, suggesting that these two manipulations increase lifespan through different mechanisms/pathways.
KW - Adipose
KW - Dietary restriction
KW - Fat
KW - Microarrays
KW - Rapamycin
KW - Transcriptome
U2 - 10.1016/j.mad.2014.07.004
DO - 10.1016/j.mad.2014.07.004
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development