Double labelling to obtain S phase subpopulations: application to determine cell kinetics of diploid cells in an aneuploid tumour
R. A. White, A. Pollack, N. H.A. Terry, M. L. Meistrich, S. Cao
We studied the cell kinetics of the murine mammary carcinoma MCa-K using iododeoxyuridine (IdUrd) and chlorodeoxyuridine (CldUrd) given at different times as independently detectable labels of S phase cells. The presence of IdUrd and CldUrd, and the amount of DNA were measured by three-colour flow cytometry making it possible to define three subpopulations within S phase and to measure the progression through the cell cycle during the time following labelling. In DNA histograms of these subpopulations, the diploid and aneuploid cells (which had a DNA index of 1.7) are essentially completely separated. From appropriate combinations of cells labelled with IdUrd only, CldUrd only, or both, it was possible to construct separate DNA distributions for the labelled diploid and aneuploid cells at the times of administration of each label. The kinetics of the diploid and aneuploid cells could be calculated for individual tumours from these two time points without having to make corrections for the presence of the second population. The diploid and aneuploid populations had indistinguishable S and G2 + M phase durations, T(S) and T(G2+M), of about 9 and 2 h; however, the potential doubling time values for the aneuploid and diploid populations were 30.2 and 101.2 h respectively.
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Diploidy Medicine & Life Sciences
Aneuploidy Medicine & Life Sciences
S Phase Medicine & Life Sciences
Idoxuridine Medicine & Life Sciences
Kinetics Medicine & Life Sciences
White, R. A., Pollack, A., Terry, N. H. A., Meistrich, M. L., & Cao, S. (1994). Double labelling to obtain S phase subpopulations: application to determine cell kinetics of diploid cells in an aneuploid tumour. Cell Proliferation, 27(3), 123-137. https://doi.org/10.1111/j.1365-2184.1994.tb01411.x
Double labelling to obtain S phase subpopulations : application to determine cell kinetics of diploid cells in an aneuploid tumour. / White, R. A.; Pollack, A.; Terry, N. H.A.; Meistrich, M. L.; Cao, S.
In: Cell Proliferation, Vol. 27, No. 3, 03.1994, p. 123-137.
White, RA, Pollack, A, Terry, NHA, Meistrich, ML & Cao, S 1994, 'Double labelling to obtain S phase subpopulations: application to determine cell kinetics of diploid cells in an aneuploid tumour', Cell Proliferation, vol. 27, no. 3, pp. 123-137. https://doi.org/10.1111/j.1365-2184.1994.tb01411.x
White RA, Pollack A, Terry NHA, Meistrich ML, Cao S. Double labelling to obtain S phase subpopulations: application to determine cell kinetics of diploid cells in an aneuploid tumour. Cell Proliferation. 1994 Mar;27(3):123-137. https://doi.org/10.1111/j.1365-2184.1994.tb01411.x
White, R. A. ; Pollack, A. ; Terry, N. H.A. ; Meistrich, M. L. ; Cao, S. / Double labelling to obtain S phase subpopulations : application to determine cell kinetics of diploid cells in an aneuploid tumour. In: Cell Proliferation. 1994 ; Vol. 27, No. 3. pp. 123-137.
@article{fdb349131f1d46a28ee3d329f5cd151a,
title = "Double labelling to obtain S phase subpopulations: application to determine cell kinetics of diploid cells in an aneuploid tumour",
abstract = "We studied the cell kinetics of the murine mammary carcinoma MCa-K using iododeoxyuridine (IdUrd) and chlorodeoxyuridine (CldUrd) given at different times as independently detectable labels of S phase cells. The presence of IdUrd and CldUrd, and the amount of DNA were measured by three-colour flow cytometry making it possible to define three subpopulations within S phase and to measure the progression through the cell cycle during the time following labelling. In DNA histograms of these subpopulations, the diploid and aneuploid cells (which had a DNA index of 1.7) are essentially completely separated. From appropriate combinations of cells labelled with IdUrd only, CldUrd only, or both, it was possible to construct separate DNA distributions for the labelled diploid and aneuploid cells at the times of administration of each label. The kinetics of the diploid and aneuploid cells could be calculated for individual tumours from these two time points without having to make corrections for the presence of the second population. The diploid and aneuploid populations had indistinguishable S and G2 + M phase durations, T(S) and T(G2+M), of about 9 and 2 h; however, the potential doubling time values for the aneuploid and diploid populations were 30.2 and 101.2 h respectively.",
author = "White, {R. A.} and A. Pollack and Terry, {N. H.A.} and Meistrich, {M. L.} and S. Cao",
journal = "Cell Proliferation",
T1 - Double labelling to obtain S phase subpopulations
T2 - application to determine cell kinetics of diploid cells in an aneuploid tumour
AU - White, R. A.
AU - Pollack, A.
AU - Terry, N. H.A.
AU - Meistrich, M. L.
AU - Cao, S.
N2 - We studied the cell kinetics of the murine mammary carcinoma MCa-K using iododeoxyuridine (IdUrd) and chlorodeoxyuridine (CldUrd) given at different times as independently detectable labels of S phase cells. The presence of IdUrd and CldUrd, and the amount of DNA were measured by three-colour flow cytometry making it possible to define three subpopulations within S phase and to measure the progression through the cell cycle during the time following labelling. In DNA histograms of these subpopulations, the diploid and aneuploid cells (which had a DNA index of 1.7) are essentially completely separated. From appropriate combinations of cells labelled with IdUrd only, CldUrd only, or both, it was possible to construct separate DNA distributions for the labelled diploid and aneuploid cells at the times of administration of each label. The kinetics of the diploid and aneuploid cells could be calculated for individual tumours from these two time points without having to make corrections for the presence of the second population. The diploid and aneuploid populations had indistinguishable S and G2 + M phase durations, T(S) and T(G2+M), of about 9 and 2 h; however, the potential doubling time values for the aneuploid and diploid populations were 30.2 and 101.2 h respectively.
AB - We studied the cell kinetics of the murine mammary carcinoma MCa-K using iododeoxyuridine (IdUrd) and chlorodeoxyuridine (CldUrd) given at different times as independently detectable labels of S phase cells. The presence of IdUrd and CldUrd, and the amount of DNA were measured by three-colour flow cytometry making it possible to define three subpopulations within S phase and to measure the progression through the cell cycle during the time following labelling. In DNA histograms of these subpopulations, the diploid and aneuploid cells (which had a DNA index of 1.7) are essentially completely separated. From appropriate combinations of cells labelled with IdUrd only, CldUrd only, or both, it was possible to construct separate DNA distributions for the labelled diploid and aneuploid cells at the times of administration of each label. The kinetics of the diploid and aneuploid cells could be calculated for individual tumours from these two time points without having to make corrections for the presence of the second population. The diploid and aneuploid populations had indistinguishable S and G2 + M phase durations, T(S) and T(G2+M), of about 9 and 2 h; however, the potential doubling time values for the aneuploid and diploid populations were 30.2 and 101.2 h respectively.
JO - Cell Proliferation
JF - Cell Proliferation