Exploring the potency of integrating semi-batch operation into lipid yield performance of Chlamydomonas sp. Tai-03
Chung Hong Tan, Pau Loke Show, Tau Chuan Ling, Dillirani Nagarajan, Duu Jong Lee, Wei-Hsin Chen, Jo-Shu Chang
Department of Aeronautics and Astronautics
International Bachelor Degree Program on Energy
Research Center for Energy Technology and Strategy
Third generation biofuels, also known as microalgal biofuels, are promising alternatives to fossil fuels. One attractive option is microalgal biodiesel as a replacement for diesel fuel. Chlamydomonas sp. Tai-03 was previously optimized for maximal lipid production for biodiesel generation, achieving biomass growth and productivity of 3.48 ± 0.04 g/L and 0.43 ± 0.01 g/L/d, with lipid content and productivity of 28.6 ± 1.41% and 124.1 ± 7.57 mg/L/d. In this study, further optimization using 5% CO2 concentration and semi-batch operation with 25% medium replacement ratio, enhanced the biomass growth and productivity to 4.15 ± 0.12 g/L and 1.23 ± 0.02 g/L/d, with lipid content and productivity of 19.4 ± 2.0% and 239.6 ± 24.8 mg/L/d. The major fatty acid methyl esters (FAMEs) were palmitic acid (C16:0), oleic acid (C18:1), and linoleic acid (C18:2). These short-chain FAMEs combined with high growth make Chlamydomonas sp. Tai-03 a suitable candidate for biodiesel synthesis.
Bioresource technology
https://doi.org/10.1016/j.biortech.2019.121331
fatty acid
Linoleic acid
Tan, C. H., Show, P. L., Ling, T. C., Nagarajan, D., Lee, D. J., Chen, W-H., & Chang, J-S. (2019). Exploring the potency of integrating semi-batch operation into lipid yield performance of Chlamydomonas sp. Tai-03. Bioresource technology, 285, [121331]. https://doi.org/10.1016/j.biortech.2019.121331
Tan, Chung Hong ; Show, Pau Loke ; Ling, Tau Chuan ; Nagarajan, Dillirani ; Lee, Duu Jong ; Chen, Wei-Hsin ; Chang, Jo-Shu. / Exploring the potency of integrating semi-batch operation into lipid yield performance of Chlamydomonas sp. Tai-03. In: Bioresource technology. 2019 ; Vol. 285.
@article{b29f5b72f8934edbb8a767881d3d9a34,
title = "Exploring the potency of integrating semi-batch operation into lipid yield performance of Chlamydomonas sp. Tai-03",
abstract = "Third generation biofuels, also known as microalgal biofuels, are promising alternatives to fossil fuels. One attractive option is microalgal biodiesel as a replacement for diesel fuel. Chlamydomonas sp. Tai-03 was previously optimized for maximal lipid production for biodiesel generation, achieving biomass growth and productivity of 3.48 ± 0.04 g/L and 0.43 ± 0.01 g/L/d, with lipid content and productivity of 28.6 ± 1.41{\%} and 124.1 ± 7.57 mg/L/d. In this study, further optimization using 5{\%} CO2 concentration and semi-batch operation with 25{\%} medium replacement ratio, enhanced the biomass growth and productivity to 4.15 ± 0.12 g/L and 1.23 ± 0.02 g/L/d, with lipid content and productivity of 19.4 ± 2.0{\%} and 239.6 ± 24.8 mg/L/d. The major fatty acid methyl esters (FAMEs) were palmitic acid (C16:0), oleic acid (C18:1), and linoleic acid (C18:2). These short-chain FAMEs combined with high growth make Chlamydomonas sp. Tai-03 a suitable candidate for biodiesel synthesis.",
author = "Tan, {Chung Hong} and Show, {Pau Loke} and Ling, {Tau Chuan} and Dillirani Nagarajan and Lee, {Duu Jong} and Wei-Hsin Chen and Jo-Shu Chang",
doi = "10.1016/j.biortech.2019.121331",
journal = "Bioresource Technology",
Tan, CH, Show, PL, Ling, TC, Nagarajan, D, Lee, DJ, Chen, W-H & Chang, J-S 2019, 'Exploring the potency of integrating semi-batch operation into lipid yield performance of Chlamydomonas sp. Tai-03', Bioresource technology, vol. 285, 121331. https://doi.org/10.1016/j.biortech.2019.121331
Exploring the potency of integrating semi-batch operation into lipid yield performance of Chlamydomonas sp. Tai-03. / Tan, Chung Hong; Show, Pau Loke; Ling, Tau Chuan; Nagarajan, Dillirani; Lee, Duu Jong; Chen, Wei-Hsin; Chang, Jo-Shu.
In: Bioresource technology, Vol. 285, 121331, 01.08.2019.
T1 - Exploring the potency of integrating semi-batch operation into lipid yield performance of Chlamydomonas sp. Tai-03
AU - Tan, Chung Hong
AU - Show, Pau Loke
AU - Ling, Tau Chuan
AU - Nagarajan, Dillirani
AU - Lee, Duu Jong
AU - Chen, Wei-Hsin
AU - Chang, Jo-Shu
N2 - Third generation biofuels, also known as microalgal biofuels, are promising alternatives to fossil fuels. One attractive option is microalgal biodiesel as a replacement for diesel fuel. Chlamydomonas sp. Tai-03 was previously optimized for maximal lipid production for biodiesel generation, achieving biomass growth and productivity of 3.48 ± 0.04 g/L and 0.43 ± 0.01 g/L/d, with lipid content and productivity of 28.6 ± 1.41% and 124.1 ± 7.57 mg/L/d. In this study, further optimization using 5% CO2 concentration and semi-batch operation with 25% medium replacement ratio, enhanced the biomass growth and productivity to 4.15 ± 0.12 g/L and 1.23 ± 0.02 g/L/d, with lipid content and productivity of 19.4 ± 2.0% and 239.6 ± 24.8 mg/L/d. The major fatty acid methyl esters (FAMEs) were palmitic acid (C16:0), oleic acid (C18:1), and linoleic acid (C18:2). These short-chain FAMEs combined with high growth make Chlamydomonas sp. Tai-03 a suitable candidate for biodiesel synthesis.
AB - Third generation biofuels, also known as microalgal biofuels, are promising alternatives to fossil fuels. One attractive option is microalgal biodiesel as a replacement for diesel fuel. Chlamydomonas sp. Tai-03 was previously optimized for maximal lipid production for biodiesel generation, achieving biomass growth and productivity of 3.48 ± 0.04 g/L and 0.43 ± 0.01 g/L/d, with lipid content and productivity of 28.6 ± 1.41% and 124.1 ± 7.57 mg/L/d. In this study, further optimization using 5% CO2 concentration and semi-batch operation with 25% medium replacement ratio, enhanced the biomass growth and productivity to 4.15 ± 0.12 g/L and 1.23 ± 0.02 g/L/d, with lipid content and productivity of 19.4 ± 2.0% and 239.6 ± 24.8 mg/L/d. The major fatty acid methyl esters (FAMEs) were palmitic acid (C16:0), oleic acid (C18:1), and linoleic acid (C18:2). These short-chain FAMEs combined with high growth make Chlamydomonas sp. Tai-03 a suitable candidate for biodiesel synthesis.
U2 - 10.1016/j.biortech.2019.121331
DO - 10.1016/j.biortech.2019.121331
JO - Bioresource Technology
JF - Bioresource Technology
Tan CH, Show PL, Ling TC, Nagarajan D, Lee DJ, Chen W-H et al. Exploring the potency of integrating semi-batch operation into lipid yield performance of Chlamydomonas sp. Tai-03. Bioresource technology. 2019 Aug 1;285. 121331. https://doi.org/10.1016/j.biortech.2019.121331
10.1016/j.biortech.2019.121331