Light scattering measurements on gangliosides: Dependence of micellar properties on molecular structure and temperature
Laura Cantù, Mario Corti, Sandro Sonnino, Guido Tettamanti
IRCCS Policlinico San Donato
Static and dynamic laser light scattering measurements on micellar aqueous solutions of gangliosides GM2, GM1, GD1a are reported. The aggregation number, the hydrodynamic radius and the micellar shape depend on the type of ganglioside and the unsaturation degree of the hydrocarbon chains. At a temperature of 25°C the molecular weights of GM2, GM1 and GD1a are 740 000, 470 000 and 418 000 DA respectively. A significant decrease of micellar size with temperature has been found for saturated GM1 in the region 25° - 40°C. The strong sensitivity of the micellar parameters to the ganglioside structure is explained by making reference to some simple model which takes into account geometrical packing considerations. By measuring the scattered light intensity at low ionic strength of the solution (0.1-30 mE) it was possible to evaluate the ganglioside micellar charge, 100 electronic units for GM2, 48 for GM1 and 60 for GD1a.
Chemistry and Physics of Lipids
gangliosides
light scattering
micellar charge
micellar shape
Dive into the research topics of 'Light scattering measurements on gangliosides: Dependence of micellar properties on molecular structure and temperature'. Together they form a unique fingerprint.
Ganglioside Chemical Compounds 100%
Gangliosides Medicine & Life Sciences 97%
Molecular Structure Medicine & Life Sciences 95%
Light Scattering Chemical Compounds 72%
Temperature Medicine & Life Sciences 56%
Ganglioside GM2 Chemical Compounds 46%
G(M2) Ganglioside Medicine & Life Sciences 41%
Cantù, L., Corti, M., Sonnino, S., & Tettamanti, G. (1986). Light scattering measurements on gangliosides: Dependence of micellar properties on molecular structure and temperature. Chemistry and Physics of Lipids, 41(3-4), 315-328. https://doi.org/10.1016/0009-3084(86)90029-0
Light scattering measurements on gangliosides : Dependence of micellar properties on molecular structure and temperature. / Cantù, Laura; Corti, Mario; Sonnino, Sandro; Tettamanti, Guido.
In: Chemistry and Physics of Lipids, Vol. 41, No. 3-4, 1986, p. 315-328.
Cantù, L, Corti, M, Sonnino, S & Tettamanti, G 1986, 'Light scattering measurements on gangliosides: Dependence of micellar properties on molecular structure and temperature', Chemistry and Physics of Lipids, vol. 41, no. 3-4, pp. 315-328. https://doi.org/10.1016/0009-3084(86)90029-0
Cantù L, Corti M, Sonnino S, Tettamanti G. Light scattering measurements on gangliosides: Dependence of micellar properties on molecular structure and temperature. Chemistry and Physics of Lipids. 1986;41(3-4):315-328. https://doi.org/10.1016/0009-3084(86)90029-0
Cantù, Laura ; Corti, Mario ; Sonnino, Sandro ; Tettamanti, Guido. / Light scattering measurements on gangliosides : Dependence of micellar properties on molecular structure and temperature. In: Chemistry and Physics of Lipids. 1986 ; Vol. 41, No. 3-4. pp. 315-328.
@article{2db8b20a647c44c7a55085be08fb2852,
title = "Light scattering measurements on gangliosides: Dependence of micellar properties on molecular structure and temperature",
abstract = "Static and dynamic laser light scattering measurements on micellar aqueous solutions of gangliosides GM2, GM1, GD1a are reported. The aggregation number, the hydrodynamic radius and the micellar shape depend on the type of ganglioside and the unsaturation degree of the hydrocarbon chains. At a temperature of 25°C the molecular weights of GM2, GM1 and GD1a are 740 000, 470 000 and 418 000 DA respectively. A significant decrease of micellar size with temperature has been found for saturated GM1 in the region 25° - 40°C. The strong sensitivity of the micellar parameters to the ganglioside structure is explained by making reference to some simple model which takes into account geometrical packing considerations. By measuring the scattered light intensity at low ionic strength of the solution (0.1-30 mE) it was possible to evaluate the ganglioside micellar charge, 100 electronic units for GM2, 48 for GM1 and 60 for GD1a.",
keywords = "gangliosides, light scattering, micellar charge, micellar shape",
author = "Laura Cant{\`u} and Mario Corti and Sandro Sonnino and Guido Tettamanti",
journal = "Chemistry and Physics of Lipids",
T1 - Light scattering measurements on gangliosides
T2 - Dependence of micellar properties on molecular structure and temperature
AU - Cantù, Laura
AU - Corti, Mario
AU - Sonnino, Sandro
AU - Tettamanti, Guido
N2 - Static and dynamic laser light scattering measurements on micellar aqueous solutions of gangliosides GM2, GM1, GD1a are reported. The aggregation number, the hydrodynamic radius and the micellar shape depend on the type of ganglioside and the unsaturation degree of the hydrocarbon chains. At a temperature of 25°C the molecular weights of GM2, GM1 and GD1a are 740 000, 470 000 and 418 000 DA respectively. A significant decrease of micellar size with temperature has been found for saturated GM1 in the region 25° - 40°C. The strong sensitivity of the micellar parameters to the ganglioside structure is explained by making reference to some simple model which takes into account geometrical packing considerations. By measuring the scattered light intensity at low ionic strength of the solution (0.1-30 mE) it was possible to evaluate the ganglioside micellar charge, 100 electronic units for GM2, 48 for GM1 and 60 for GD1a.
AB - Static and dynamic laser light scattering measurements on micellar aqueous solutions of gangliosides GM2, GM1, GD1a are reported. The aggregation number, the hydrodynamic radius and the micellar shape depend on the type of ganglioside and the unsaturation degree of the hydrocarbon chains. At a temperature of 25°C the molecular weights of GM2, GM1 and GD1a are 740 000, 470 000 and 418 000 DA respectively. A significant decrease of micellar size with temperature has been found for saturated GM1 in the region 25° - 40°C. The strong sensitivity of the micellar parameters to the ganglioside structure is explained by making reference to some simple model which takes into account geometrical packing considerations. By measuring the scattered light intensity at low ionic strength of the solution (0.1-30 mE) it was possible to evaluate the ganglioside micellar charge, 100 electronic units for GM2, 48 for GM1 and 60 for GD1a.
KW - gangliosides
KW - light scattering
KW - micellar charge
KW - micellar shape
JO - Chemistry and Physics of Lipids
JF - Chemistry and Physics of Lipids