Evolutionary trajectory of turnip mosaic virus populations adapting to a new host
Kazusato Ohshima1, Sadayuki Akaishi1, Hiromi Kajiyama1, Ryoko Koga1, Adrian J. Gibbs2
Affiliations: 1 1Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan 2 27 Hutt Street, Yarralumla, ACT 2600, Australia
CorrespondenceKazusato Ohshima
[email protected]
Published: 01 March 2010 https://doi.org/10.1099/vir.0.016055-0
Supplementary Material (2)
Little is known about how some plant viruses establish successful cross-species transmission whilst others do not; the genetic basis for adaptation is largely unknown. This study investigated the genetic changes that occurred using the progeny of an infectious clone, p35Tunos, derived from the turnip mosaic virus (TuMV) UK 1 isolate, which has a Brassica host type, but rarely infects Raphanus systemically and then only asymptomatically. The genetic trajectory leading to viral adaptation was studied in a TuMV isolate passaged in Nicotiana benthamiana (parental), Brassica rapa, the old (susceptible) host and Raphanus sativus, the new (almost insusceptible) host. Almost-complete consensus genomic sequences were obtained by RT-PCR of viral populations passaged up to 35 times together with 59 full sequences of 578 200 nt. There were significant differences in the nucleotide and encoded amino acid changes in the consensus genomes from the old and new hosts. Furthermore, a 3264 nt region corresponding to nt 3222–6485 of the UK 1 genome was cloned, and 269 clones from 23 populations were sequenced; this region covered 33 % of the genome and represented a total of 878 016 nt. The results showed that the nucleotide diversity and the non-synonymous/synonymous ratio of the populations from the new host were higher than those from the old host. An analysis of molecular variance showed significant differences among the populations from the old and new hosts. As far as is known, this is the first report comparing the evolutionary trajectory dynamics of plant virus populations in old and new hosts.
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J. Gen. Virol. 91, 788 (2010); https://doi.org/10.1099/vir.0.016055-0
Supplementary Table S1. Shared, fixed and parallel evolution sites in the consensus and quasispecies sequences [ PDF] (178 KB; includes supplementary references)
Supplementary Fig. S1. Sequence changes found between p35Tunos and the variants of TuMV during passage in the old host, B. rapa , and the new host, R. sativus [ PDF] (88 KB)
10.1099/vir.0.016055-0