From Mar. 22, 1990 to May 23, 1990
comparable to guideline study with acceptable restrictions
EU Method C.1 (Acute Toxicity for Fish)
No analytical monitoring. Tween 20 has been used as emulsifer
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Calculated amounts of the test substance and 60 mg Tween 20 per tank to produce the desired test concentrations were added to the water in each tank and were homogeniously distributed.
The test substance seemed to be homogeneously distributed in the test vessels for all concentrations and at the start and the end of exposure.
- Concentrations (nominal): 10, 18, 32 58 and 100 mg/L
- Controls: Blank (dechlorinated tap water) Vehicle (4 mg Tween 20 / L)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 20
Danio rerio (previous name: Brachydanio rerio)
- Common name: Zebra-Fish
- Strain: Brachydanio rerio
- Source: West Aquarium / D-3422 Bad Lauterberg
- Length at study initiation (length definition, mean, range and SD): 29 mm (27 - 30 mm)
- Weight at study initiation (mean and range, SD): 0.23 g (0.21 - 0.26 g)
- Feeding during test: No
- Lighting: Fluorescent light, 16 hours daily
- Aeration: none
- Tempreature: 30 to 24°C within +/-1°C
192 mg CaCO3 / L
Salinity:
Nominal: 10, 18, 32, 58 and 100 mg/L
- Test vessel: glass aquaria of 20 liters filled with 15 liters. (36/22/25 cm)
- No. of organisms per vessel: 10
- No. of vessels per vehicle control (replicates): 1
- Source/preparation of dilution water: Dechlorinated tap water
- Intervals of water quality measurement: oxygen content, pH and temperature were measured at 0, 24, 48, 72 and 96 hours.
- Adjustment of pH: no
- Photoperiod: 16 hours light, 8 hours dark
- Light intensity: Fluorescent light
- Temperature: 20 to 24°C +/- 1°C
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortalities were recorded at 24, 48, 72 and 96 hours. Symptons of toxicity were observed daily.
mortality (fish)
other: 95% CL 20 - 28 mg/L; calculated
- Behavioural abnormalities: At 18 mg/L swimming behaviour and loss of equilibrium were recorded as moderate symptoms.
- Mortality of control: 0% mortality in blank or vehicle controls.
The LC50 values were calculated according to BERKSON, JASA 48 (1953), 569-599.
The LC-values were graphically determined on gausso-logarithmic probability paper.
Sublethal observations / clinical signs:
Effect concentration [mg/L]
LC50 (graphically determined) 24
The LC50 (96h) of the test substance is 24 mg/L (nominal concentration).
In this guideline (EU C.1) study, the 96 hour LC50 to fish for the test material (EC 213-426-9) was determined to be 24 mg/l. The test was conducted in Danio rerio (previous name: Brachydanio rerio) under static conditions (freshwater), with test concentrations ranging from 10 to 100 mg/l. The study result does not trigger classification as hazardous for the aquatic environment under the the conditions of the Classification, Labelling, and Packaging (CLP) regulation (1272/2008), as the test material is readily biodegradable and has a BCF <500.
Study conducted to recognised testing guideline.
Effect concentration:
In a toxicity study on fish according to the Official Journal of the European Communities L251, vol.27, C-01, 19/09/1984 (CIBA-GEIGY Ltd., 1990), the toxicity of the test item was determined. Zebra fish were used as test organisms and the LC50 (96 h) determined in the test was 24 mg/L.