Global Symmetry: Dihedral - D3 (3D View)
Global Stoichiometry: Homo 6-mer - A6
6P2T
BshB from Bacillus subtilis complexed with citrate
DOI: 10.2210/pdb6P2T/pdb
Classification: HYDROLASE
Organism(s): Bacillus subtilis (strain 168)
Deposition Author(s): Cook, P.D., Meloche, C.E.
Resolution: 1.853 Å
X-ray crystallographic structure of BshB, the zinc-dependent deacetylase involved in bacillithiol biosynthesis.
Woodward, R.L., Castleman, M.M., Meloche, C.E., Karpen, M.E., Carlson, C.G., Yobi, W.H., Jepsen, J.C., Lewis, B.W., Zarnosky, B.N., Cook, P.D.
(2019) Protein Sci. --: --
DOI: 10.1002/pro.3808
6ULL
Many gram-positive bacteria produce bacillithiol to aid in the maintenance of redox homeostasis and degradation of toxic compounds, including the antibiotic fosfomycin. Bacillithiol is produced via a three-enzyme pathway that includes the action of t ...
Many gram-positive bacteria produce bacillithiol to aid in the maintenance of redox homeostasis and degradation of toxic compounds, including the antibiotic fosfomycin. Bacillithiol is produced via a three-enzyme pathway that includes the action of the zinc-dependent deacetylase BshB. Previous studies identified conserved aspartate and histidine residues within the active site that are involved in metal binding and catalysis, but the enzymatic mechanism is not fully understood. Here we report two X-ray crystallographic structures of BshB from Bacillus subtilis that provide insight into the BshB catalytic mechanism.
Department of Chemistry and Biochemistry, University of Mount Union, Alliance, Ohio.,Department of Chemistry, Grand Valley State University, Allendale, Michigan.
N-acetyl-alpha-D-glucosaminyl L-malate deacetylase 1
256 Bacillus subtilis (strain 168) Mutation(s): 0
Gene Names: bshB1 (jojG, ypjG)
EC: 3.5.1.-
Find proteins for P42981 (Bacillus subtilis (strain 168))
Query on ZN
ZINC ION
PTFCDOFLOPIGGS-UHFFFAOYSA-N
Query on NA
SODIUM ION
FKNQFGJONOIPTF-UHFFFAOYSA-N
Query on CIT
C6 H8 O7
KRKNYBCHXYNGOX-UHFFFAOYSA-N
Space Group: H 3 2
MOSFLM data reduction
Aimless data scaling
PHASER phasing
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) United States --