Gene expression in blood of subjects with Duchenne muscular dystrophy
Brenda Wong, Donald L. Gilbert, Wynn L. Walker, Isaac H. Liao, Lisa Lit, Boryana Stamova, Glen Jickling, Michelle L Apperson, Frank R Sharp
The objective of this study was to examine RNA expression in blood of subjects with Duchenne muscular dystrophy (DMD). Whole blood was collected into PAX gene tubes and RNA was isolated for 3- to 20-year-old males with DMD (n∈=∈34) and for age- and gender-matched normal healthy controls (n∈=∈21). DMD was confirmed by genetic testing in all subjects. RNA expression was measured on Affymetrix whole-genome human U133 Plus 2.0 GeneChips. Using a Benjamini-Hochberg false discovery rate of 0.05 to correct for multiple comparisons, an unpaired t test for DMD versus controls yielded 10,763 regulated probes with no fold change cutoff, 1,467 probes with >|1.5|-fold change, 191 probes with >|2.0|-fold change, and 59 probes with a >|2.5|-fold change. These genes (probes) separated DMD from controls using cluster analyses. Almost all of the genes regulated in peripheral blood were different from the genes reported to be regulated in diseased muscle of subjects with DMD. It is proposed that the genes regulated in blood of subjects with Duchenne muscular dystrophy are indicative, at least in part, of the immune response to the diseased DMD muscle. The regulated genes might be used to monitor therapy or provide novel targets for immune-directed therapy for DMD.
Fingerprint Dive into the research topics of 'Gene expression in blood of subjects with Duchenne muscular dystrophy'. Together they form a unique fingerprint.
Duchenne Muscular Dystrophy Medicine & Life Sciences
Genetic Testing Medicine & Life Sciences
Wong, B., Gilbert, D. L., Walker, W. L., Liao, I. H., Lit, L., Stamova, B., Jickling, G., Apperson, M. L., & Sharp, F. R. (2009). Gene expression in blood of subjects with Duchenne muscular dystrophy. Neurogenetics, 10(2), 117-125. https://doi.org/10.1007/s10048-008-0167-8
Gene expression in blood of subjects with Duchenne muscular dystrophy. / Wong, Brenda; Gilbert, Donald L.; Walker, Wynn L.; Liao, Isaac H.; Lit, Lisa; Stamova, Boryana; Jickling, Glen; Apperson, Michelle L; Sharp, Frank R.
In: Neurogenetics, Vol. 10, No. 2, 04.2009, p. 117-125.
Wong, B, Gilbert, DL, Walker, WL, Liao, IH, Lit, L, Stamova, B, Jickling, G, Apperson, ML & Sharp, FR 2009, 'Gene expression in blood of subjects with Duchenne muscular dystrophy', Neurogenetics, vol. 10, no. 2, pp. 117-125. https://doi.org/10.1007/s10048-008-0167-8
Wong B, Gilbert DL, Walker WL, Liao IH, Lit L, Stamova B et al. Gene expression in blood of subjects with Duchenne muscular dystrophy. Neurogenetics. 2009 Apr;10(2):117-125. https://doi.org/10.1007/s10048-008-0167-8
Wong, Brenda ; Gilbert, Donald L. ; Walker, Wynn L. ; Liao, Isaac H. ; Lit, Lisa ; Stamova, Boryana ; Jickling, Glen ; Apperson, Michelle L ; Sharp, Frank R. / Gene expression in blood of subjects with Duchenne muscular dystrophy. In: Neurogenetics. 2009 ; Vol. 10, No. 2. pp. 117-125.
@article{422407533e4c4a83bd02fdc9a7e52cf4,
title = "Gene expression in blood of subjects with Duchenne muscular dystrophy",
abstract = "The objective of this study was to examine RNA expression in blood of subjects with Duchenne muscular dystrophy (DMD). Whole blood was collected into PAX gene tubes and RNA was isolated for 3- to 20-year-old males with DMD (n∈=∈34) and for age- and gender-matched normal healthy controls (n∈=∈21). DMD was confirmed by genetic testing in all subjects. RNA expression was measured on Affymetrix whole-genome human U133 Plus 2.0 GeneChips. Using a Benjamini-Hochberg false discovery rate of 0.05 to correct for multiple comparisons, an unpaired t test for DMD versus controls yielded 10,763 regulated probes with no fold change cutoff, 1,467 probes with >|1.5|-fold change, 191 probes with >|2.0|-fold change, and 59 probes with a >|2.5|-fold change. These genes (probes) separated DMD from controls using cluster analyses. Almost all of the genes regulated in peripheral blood were different from the genes reported to be regulated in diseased muscle of subjects with DMD. It is proposed that the genes regulated in blood of subjects with Duchenne muscular dystrophy are indicative, at least in part, of the immune response to the diseased DMD muscle. The regulated genes might be used to monitor therapy or provide novel targets for immune-directed therapy for DMD.",
keywords = "Blood, Immune, Microarrays, Muscle, Muscular dystrophy",
author = "Brenda Wong and Gilbert, {Donald L.} and Walker, {Wynn L.} and Liao, {Isaac H.} and Lisa Lit and Boryana Stamova and Glen Jickling and Apperson, {Michelle L} and Sharp, {Frank R}",
journal = "Neurogenetics",
T1 - Gene expression in blood of subjects with Duchenne muscular dystrophy
AU - Wong, Brenda
AU - Gilbert, Donald L.
AU - Walker, Wynn L.
AU - Liao, Isaac H.
AU - Lit, Lisa
AU - Stamova, Boryana
AU - Jickling, Glen
AU - Apperson, Michelle L
AU - Sharp, Frank R
N2 - The objective of this study was to examine RNA expression in blood of subjects with Duchenne muscular dystrophy (DMD). Whole blood was collected into PAX gene tubes and RNA was isolated for 3- to 20-year-old males with DMD (n∈=∈34) and for age- and gender-matched normal healthy controls (n∈=∈21). DMD was confirmed by genetic testing in all subjects. RNA expression was measured on Affymetrix whole-genome human U133 Plus 2.0 GeneChips. Using a Benjamini-Hochberg false discovery rate of 0.05 to correct for multiple comparisons, an unpaired t test for DMD versus controls yielded 10,763 regulated probes with no fold change cutoff, 1,467 probes with >|1.5|-fold change, 191 probes with >|2.0|-fold change, and 59 probes with a >|2.5|-fold change. These genes (probes) separated DMD from controls using cluster analyses. Almost all of the genes regulated in peripheral blood were different from the genes reported to be regulated in diseased muscle of subjects with DMD. It is proposed that the genes regulated in blood of subjects with Duchenne muscular dystrophy are indicative, at least in part, of the immune response to the diseased DMD muscle. The regulated genes might be used to monitor therapy or provide novel targets for immune-directed therapy for DMD.
AB - The objective of this study was to examine RNA expression in blood of subjects with Duchenne muscular dystrophy (DMD). Whole blood was collected into PAX gene tubes and RNA was isolated for 3- to 20-year-old males with DMD (n∈=∈34) and for age- and gender-matched normal healthy controls (n∈=∈21). DMD was confirmed by genetic testing in all subjects. RNA expression was measured on Affymetrix whole-genome human U133 Plus 2.0 GeneChips. Using a Benjamini-Hochberg false discovery rate of 0.05 to correct for multiple comparisons, an unpaired t test for DMD versus controls yielded 10,763 regulated probes with no fold change cutoff, 1,467 probes with >|1.5|-fold change, 191 probes with >|2.0|-fold change, and 59 probes with a >|2.5|-fold change. These genes (probes) separated DMD from controls using cluster analyses. Almost all of the genes regulated in peripheral blood were different from the genes reported to be regulated in diseased muscle of subjects with DMD. It is proposed that the genes regulated in blood of subjects with Duchenne muscular dystrophy are indicative, at least in part, of the immune response to the diseased DMD muscle. The regulated genes might be used to monitor therapy or provide novel targets for immune-directed therapy for DMD.
KW - Blood
KW - Immune
KW - Microarrays
KW - Muscle
KW - Muscular dystrophy
JO - Neurogenetics
JF - Neurogenetics