1α,25-Dihydroxyvitamin D3 regulates the expression of carbonic anhydrase II in nonerythroid avian bone marrow cells
Agnes Billecocq, Janet Rettig Emanuel, Robert Levenson, Roland Baron
Department of Neural and Behavioral Sciences
Penn State Neuroscience Institute
1α,25-Dihydroxy vitamin D3 [1,25(OH)2D3], the active metabolite of the steroid hormone vitamin D, is a potent regulator of macrophage and osteoclast differentiation. The mature osteoclast, unlike the circulating monocyte or the tissue macrophage, expresses high levels of carbonic anhydrase II (CAII). This enzyme generates protons and bicarbonate from water and carbon dioxide and is involved in bone resorption and acid-base regulation. To test whether 1,25(OH)2D3 could induce the differentiation of myelomonocytic precursors toward osteoclasts rather than macrophages, we analyzed its effects on the expression of CAII in bone marrow cultures containing precursors common to both cell types. The expression of CAII was markedly increased by 1,25(OH)2D3 in a dose-and time-dependent manner. In bone marrow, this increase occurred at the mRNA and protein levels and was detectable as early as 24 hr after stimulation. 1,25(OH)2D3 was also found to induce CAII expression in a transformed myelomonocytic avian cell line. These results suggest that 1,25(OH)2D3 regulates the level at which myelomonocytic precursors express CAII, an enzyme that is involved in the function of the mature osteoclast.
https://doi.org/10.1073/pnas.87.16.6470
10.1073/pnas.87.16.6470
Dive into the research topics of '1α,25-Dihydroxyvitamin D3 regulates the expression of carbonic anhydrase II in nonerythroid avian bone marrow cells'. Together they form a unique fingerprint.
Carbonic Anhydrase II Medicine & Life Sciences 100%
Calcitriol Medicine & Life Sciences 76%
Bone Marrow Cells Medicine & Life Sciences 62%
Osteoclasts Medicine & Life Sciences 54%
Macrophages Medicine & Life Sciences 27%
Bone Marrow Medicine & Life Sciences 19%
dihydroxy-vitamin D3 Medicine & Life Sciences 18%
Bicarbonates Medicine & Life Sciences 14%
Billecocq, A., Emanuel, J. R., Levenson, R., & Baron, R. (1990). 1α,25-Dihydroxyvitamin D3 regulates the expression of carbonic anhydrase II in nonerythroid avian bone marrow cells. Proceedings of the National Academy of Sciences of the United States of America, 87(16), 6470-6474. https://doi.org/10.1073/pnas.87.16.6470
Billecocq, Agnes ; Emanuel, Janet Rettig ; Levenson, Robert et al. / 1α,25-Dihydroxyvitamin D3 regulates the expression of carbonic anhydrase II in nonerythroid avian bone marrow cells. In: Proceedings of the National Academy of Sciences of the United States of America. 1990 ; Vol. 87, No. 16. pp. 6470-6474.
@article{42d14f05c14c457b99656c4840a0a307,
title = "1α,25-Dihydroxyvitamin D3 regulates the expression of carbonic anhydrase II in nonerythroid avian bone marrow cells",
abstract = "1α,25-Dihydroxy vitamin D3 [1,25(OH)2D3], the active metabolite of the steroid hormone vitamin D, is a potent regulator of macrophage and osteoclast differentiation. The mature osteoclast, unlike the circulating monocyte or the tissue macrophage, expresses high levels of carbonic anhydrase II (CAII). This enzyme generates protons and bicarbonate from water and carbon dioxide and is involved in bone resorption and acid-base regulation. To test whether 1,25(OH)2D3 could induce the differentiation of myelomonocytic precursors toward osteoclasts rather than macrophages, we analyzed its effects on the expression of CAII in bone marrow cultures containing precursors common to both cell types. The expression of CAII was markedly increased by 1,25(OH)2D3 in a dose-and time-dependent manner. In bone marrow, this increase occurred at the mRNA and protein levels and was detectable as early as 24 hr after stimulation. 1,25(OH)2D3 was also found to induce CAII expression in a transformed myelomonocytic avian cell line. These results suggest that 1,25(OH)2D3 regulates the level at which myelomonocytic precursors express CAII, an enzyme that is involved in the function of the mature osteoclast.",
author = "Agnes Billecocq and Emanuel, {Janet Rettig} and Robert Levenson and Roland Baron",
doi = "10.1073/pnas.87.16.6470",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
publisher = "National Academy of Sciences",
Billecocq, A, Emanuel, JR, Levenson, R & Baron, R 1990, '1α,25-Dihydroxyvitamin D3 regulates the expression of carbonic anhydrase II in nonerythroid avian bone marrow cells', Proceedings of the National Academy of Sciences of the United States of America, vol. 87, no. 16, pp. 6470-6474. https://doi.org/10.1073/pnas.87.16.6470
1α,25-Dihydroxyvitamin D3 regulates the expression of carbonic anhydrase II in nonerythroid avian bone marrow cells. / Billecocq, Agnes; Emanuel, Janet Rettig; Levenson, Robert et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 16, 08.1990, p. 6470-6474.
T1 - 1α,25-Dihydroxyvitamin D3 regulates the expression of carbonic anhydrase II in nonerythroid avian bone marrow cells
AU - Billecocq, Agnes
AU - Emanuel, Janet Rettig
AU - Levenson, Robert
AU - Baron, Roland
N2 - 1α,25-Dihydroxy vitamin D3 [1,25(OH)2D3], the active metabolite of the steroid hormone vitamin D, is a potent regulator of macrophage and osteoclast differentiation. The mature osteoclast, unlike the circulating monocyte or the tissue macrophage, expresses high levels of carbonic anhydrase II (CAII). This enzyme generates protons and bicarbonate from water and carbon dioxide and is involved in bone resorption and acid-base regulation. To test whether 1,25(OH)2D3 could induce the differentiation of myelomonocytic precursors toward osteoclasts rather than macrophages, we analyzed its effects on the expression of CAII in bone marrow cultures containing precursors common to both cell types. The expression of CAII was markedly increased by 1,25(OH)2D3 in a dose-and time-dependent manner. In bone marrow, this increase occurred at the mRNA and protein levels and was detectable as early as 24 hr after stimulation. 1,25(OH)2D3 was also found to induce CAII expression in a transformed myelomonocytic avian cell line. These results suggest that 1,25(OH)2D3 regulates the level at which myelomonocytic precursors express CAII, an enzyme that is involved in the function of the mature osteoclast.
AB - 1α,25-Dihydroxy vitamin D3 [1,25(OH)2D3], the active metabolite of the steroid hormone vitamin D, is a potent regulator of macrophage and osteoclast differentiation. The mature osteoclast, unlike the circulating monocyte or the tissue macrophage, expresses high levels of carbonic anhydrase II (CAII). This enzyme generates protons and bicarbonate from water and carbon dioxide and is involved in bone resorption and acid-base regulation. To test whether 1,25(OH)2D3 could induce the differentiation of myelomonocytic precursors toward osteoclasts rather than macrophages, we analyzed its effects on the expression of CAII in bone marrow cultures containing precursors common to both cell types. The expression of CAII was markedly increased by 1,25(OH)2D3 in a dose-and time-dependent manner. In bone marrow, this increase occurred at the mRNA and protein levels and was detectable as early as 24 hr after stimulation. 1,25(OH)2D3 was also found to induce CAII expression in a transformed myelomonocytic avian cell line. These results suggest that 1,25(OH)2D3 regulates the level at which myelomonocytic precursors express CAII, an enzyme that is involved in the function of the mature osteoclast.
U2 - 10.1073/pnas.87.16.6470
DO - 10.1073/pnas.87.16.6470
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
Billecocq A, Emanuel JR, Levenson R, Baron R. 1α,25-Dihydroxyvitamin D3 regulates the expression of carbonic anhydrase II in nonerythroid avian bone marrow cells. Proceedings of the National Academy of Sciences of the United States of America. 1990 Aug;87(16):6470-6474. doi: 10.1073/pnas.87.16.6470