Molecular and functional characterization of the Gulf toadfish serotonin transporter SLC6A4
Molly H.B. Amador, Danielle M Mcdonald
Marine Biology and Ecology
The serotonin transporter (SERT) functions in the uptake of the neurotransmitter serotonin (5-HT) from the extracellular milieu and is the molecular target of the selective serotonin re-uptake inhibitors (SSRIs), a common group of anti-depressants. The current study comprehensively assesses the sequence, tissue distribution, transport kinetics and physiological function of a teleost SERT. The 2022 bp toadfish SERT sequence encodes a protein of 673 amino acids, which shows 83% similarity to zebrafish SERT and groups with SERT of other teleosts in phylogenetic analysis. SERT mRNA is ubiquitous in tissues and is expressed at high levels in the heart and, within the brain, in the cerebellum. SERT cRNA expressed in Xenopus laevis oocytes demonstrates a Km value of 2.08± 0.45 μmol l-1, similar to previously reported Km values for zebrafish and human SERT. Acute systemic blockade of SERT by intraperitoneal administration of the SSRI fluoxetine (FLX) produces a dose-dependent increase in plasma 5-HT, indicating effective inhibition of 5-HT uptake from the circulation. As teleosts lack platelets, which are important 5-HT sequestration sites in mammals, the FLX-induced increase in plasma 5-HT suggests that toadfish tissues may normally be responsible for maintaining low 5-HT concentrations in the bloodstream.
jeb170928
https://doi.org/10.1242/jeb.170928
5-HT
5-HTT
5-Hydroxytryptamine
MRNA expression
Opsanus beta
Transport kinetics
10.1242/jeb.170928
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Batrachoidiformes Medicine & Life Sciences
Opsanus beta Agriculture & Biology
Serotonin Plasma Membrane Transport Proteins Medicine & Life Sciences
teleost Earth & Environmental Sciences
serotonin Agriculture & Biology
transporters Agriculture & Biology
gulf Earth & Environmental Sciences
tissue Earth & Environmental Sciences
Amador, M. H. B., & Mcdonald, D. M. (2018). Molecular and functional characterization of the Gulf toadfish serotonin transporter SLC6A4. Journal of Experimental Biology, 221(7), [jeb170928]. https://doi.org/10.1242/jeb.170928
Molecular and functional characterization of the Gulf toadfish serotonin transporter SLC6A4. / Amador, Molly H.B.; Mcdonald, Danielle M.
In: Journal of Experimental Biology, Vol. 221, No. 7, jeb170928, 01.04.2018.
Amador, MHB & Mcdonald, DM 2018, 'Molecular and functional characterization of the Gulf toadfish serotonin transporter SLC6A4', Journal of Experimental Biology, vol. 221, no. 7, jeb170928. https://doi.org/10.1242/jeb.170928
Amador MHB, Mcdonald DM. Molecular and functional characterization of the Gulf toadfish serotonin transporter SLC6A4. Journal of Experimental Biology. 2018 Apr 1;221(7). jeb170928. https://doi.org/10.1242/jeb.170928
Amador, Molly H.B. ; Mcdonald, Danielle M. / Molecular and functional characterization of the Gulf toadfish serotonin transporter SLC6A4. In: Journal of Experimental Biology. 2018 ; Vol. 221, No. 7.
@article{528e7034733742959890f0318c7fbb29,
title = "Molecular and functional characterization of the Gulf toadfish serotonin transporter SLC6A4",
abstract = "The serotonin transporter (SERT) functions in the uptake of the neurotransmitter serotonin (5-HT) from the extracellular milieu and is the molecular target of the selective serotonin re-uptake inhibitors (SSRIs), a common group of anti-depressants. The current study comprehensively assesses the sequence, tissue distribution, transport kinetics and physiological function of a teleost SERT. The 2022 bp toadfish SERT sequence encodes a protein of 673 amino acids, which shows 83% similarity to zebrafish SERT and groups with SERT of other teleosts in phylogenetic analysis. SERT mRNA is ubiquitous in tissues and is expressed at high levels in the heart and, within the brain, in the cerebellum. SERT cRNA expressed in Xenopus laevis oocytes demonstrates a Km value of 2.08± 0.45 μmol l-1, similar to previously reported Km values for zebrafish and human SERT. Acute systemic blockade of SERT by intraperitoneal administration of the SSRI fluoxetine (FLX) produces a dose-dependent increase in plasma 5-HT, indicating effective inhibition of 5-HT uptake from the circulation. As teleosts lack platelets, which are important 5-HT sequestration sites in mammals, the FLX-induced increase in plasma 5-HT suggests that toadfish tissues may normally be responsible for maintaining low 5-HT concentrations in the bloodstream.",
keywords = "5-HT, 5-HTT, 5-Hydroxytryptamine, MRNA expression, Opsanus beta, Transport kinetics",
author = "Amador, {Molly H.B.} and Mcdonald, {Danielle M}",
doi = "10.1242/jeb.170928",
journal = "Journal of Experimental Biology",
publisher = "Company of Biologists Ltd",
T1 - Molecular and functional characterization of the Gulf toadfish serotonin transporter SLC6A4
AU - Amador, Molly H.B.
AU - Mcdonald, Danielle M
N2 - The serotonin transporter (SERT) functions in the uptake of the neurotransmitter serotonin (5-HT) from the extracellular milieu and is the molecular target of the selective serotonin re-uptake inhibitors (SSRIs), a common group of anti-depressants. The current study comprehensively assesses the sequence, tissue distribution, transport kinetics and physiological function of a teleost SERT. The 2022 bp toadfish SERT sequence encodes a protein of 673 amino acids, which shows 83% similarity to zebrafish SERT and groups with SERT of other teleosts in phylogenetic analysis. SERT mRNA is ubiquitous in tissues and is expressed at high levels in the heart and, within the brain, in the cerebellum. SERT cRNA expressed in Xenopus laevis oocytes demonstrates a Km value of 2.08± 0.45 μmol l-1, similar to previously reported Km values for zebrafish and human SERT. Acute systemic blockade of SERT by intraperitoneal administration of the SSRI fluoxetine (FLX) produces a dose-dependent increase in plasma 5-HT, indicating effective inhibition of 5-HT uptake from the circulation. As teleosts lack platelets, which are important 5-HT sequestration sites in mammals, the FLX-induced increase in plasma 5-HT suggests that toadfish tissues may normally be responsible for maintaining low 5-HT concentrations in the bloodstream.
AB - The serotonin transporter (SERT) functions in the uptake of the neurotransmitter serotonin (5-HT) from the extracellular milieu and is the molecular target of the selective serotonin re-uptake inhibitors (SSRIs), a common group of anti-depressants. The current study comprehensively assesses the sequence, tissue distribution, transport kinetics and physiological function of a teleost SERT. The 2022 bp toadfish SERT sequence encodes a protein of 673 amino acids, which shows 83% similarity to zebrafish SERT and groups with SERT of other teleosts in phylogenetic analysis. SERT mRNA is ubiquitous in tissues and is expressed at high levels in the heart and, within the brain, in the cerebellum. SERT cRNA expressed in Xenopus laevis oocytes demonstrates a Km value of 2.08± 0.45 μmol l-1, similar to previously reported Km values for zebrafish and human SERT. Acute systemic blockade of SERT by intraperitoneal administration of the SSRI fluoxetine (FLX) produces a dose-dependent increase in plasma 5-HT, indicating effective inhibition of 5-HT uptake from the circulation. As teleosts lack platelets, which are important 5-HT sequestration sites in mammals, the FLX-induced increase in plasma 5-HT suggests that toadfish tissues may normally be responsible for maintaining low 5-HT concentrations in the bloodstream.
KW - 5-HT
KW - 5-HTT
KW - 5-Hydroxytryptamine
KW - MRNA expression
KW - Opsanus beta
KW - Transport kinetics
U2 - 10.1242/jeb.170928
DO - 10.1242/jeb.170928
JO - Journal of Experimental Biology
JF - Journal of Experimental Biology
M1 - jeb170928