Constitutive Nitric Oxide Synthase Is Expressed and Nitric Oxide-Mediated Relaxation Is Preserved in Retrieved Human Aortocoronary Vein Grafts
Mark G. Davies, Dan E. Berkowitz, Per Otto Hagen
Although little is known about the endothelial cell function of human saphenous vein coronary artery bypass grafts, there is evidence to suggest that receptor-activated, endothelial-dependent relaxation mediated by nitric oxide is impaired. This study examines the expression and function of endothelial cell constitutive nitric oxide synthase (cNOS) of aortocoronary vein bypass grafts and human saphenous veins obtained from 10 patients undergoing repeat coronary artery bypass grafting for recurrent ischemic symptoms. Following precontraction with norepinephrine (10-5 M), responses to acetylcholine (receptor-mediated, endothelium-dependent), calcium ionophore (A23187; receptor-independent, endothelium-dependent), and sodium nitroprusside (endothelium-independent) were assessed. Following total RNA extraction using phenol/guanidinium isothiocyanate from specimens of human saphenous vein and vein graft, a quantitative RNase Protection Assay (RPA) was performed using a cRNA riboprobe corresponding to a fragment of the human endothelial cell cNOS gone. Histologically, the vein grafts showed both intimal hyperplasia development and focal atherosclerosis formation compared to the saphenous veins. Scanning electron microscopy of the saphenous veins and the vein grafts showed an intact endothelium. Precontracted vein grafts did not relax in response to acetylcholine; in contrast, the saphenous vein relaxed in a dose-dependent manner to reach a maximal relaxation of 19 ± 4% precontracted tension. Saphenous veins and vein grafts relaxed in response to A23187 with maximal relaxation of 92 ± 5 and 73 ± 13%, respectively. Both vessels relaxed in a dose dependent manner to sodium nitroprusside. RPA normalized to β-actin showed similar levels of expression of endothelial cell cNOS equivalent to 1 pg of sense RNA in both the saphenous vein and vein graft. These data demonstrate that the endothelium of coronary artery vein grafts shows both expression and function of endothelial cell cNOS. However, the impaired NO-mediated relaxation of aortocoronary vein grafts in response to acetylcholine appears to be due to altered receptor activation of the endothelial cNOS system.
Journal of Surgical Research
https://doi.org/10.1006/jsre.1995.1116
10.1006/jsre.1995.1116
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Nitric Oxide Synthase Medicine & Life Sciences 100%
Saphenous Vein Medicine & Life Sciences 92%
Veins Medicine & Life Sciences 86%
Nitric Oxide Medicine & Life Sciences 83%
Nitric Oxide Synthase Type III Medicine & Life Sciences 58%
Endothelium Medicine & Life Sciences 45%
Endothelial Cells Medicine & Life Sciences 38%
Davies, M. G., Berkowitz, D. E., & Hagen, P. O. (1995). Constitutive Nitric Oxide Synthase Is Expressed and Nitric Oxide-Mediated Relaxation Is Preserved in Retrieved Human Aortocoronary Vein Grafts. Journal of Surgical Research, 58(6), 732-738. https://doi.org/10.1006/jsre.1995.1116
Constitutive Nitric Oxide Synthase Is Expressed and Nitric Oxide-Mediated Relaxation Is Preserved in Retrieved Human Aortocoronary Vein Grafts. / Davies, Mark G.; Berkowitz, Dan E.; Hagen, Per Otto.
In: Journal of Surgical Research, Vol. 58, No. 6, 06.1995, p. 732-738.
Davies, MG, Berkowitz, DE & Hagen, PO 1995, 'Constitutive Nitric Oxide Synthase Is Expressed and Nitric Oxide-Mediated Relaxation Is Preserved in Retrieved Human Aortocoronary Vein Grafts', Journal of Surgical Research, vol. 58, no. 6, pp. 732-738. https://doi.org/10.1006/jsre.1995.1116
Davies MG, Berkowitz DE, Hagen PO. Constitutive Nitric Oxide Synthase Is Expressed and Nitric Oxide-Mediated Relaxation Is Preserved in Retrieved Human Aortocoronary Vein Grafts. Journal of Surgical Research. 1995 Jun;58(6):732-738. https://doi.org/10.1006/jsre.1995.1116
Davies, Mark G. ; Berkowitz, Dan E. ; Hagen, Per Otto. / Constitutive Nitric Oxide Synthase Is Expressed and Nitric Oxide-Mediated Relaxation Is Preserved in Retrieved Human Aortocoronary Vein Grafts. In: Journal of Surgical Research. 1995 ; Vol. 58, No. 6. pp. 732-738.
@article{65e07bb08ca143b6859169082c87ed30,
title = "Constitutive Nitric Oxide Synthase Is Expressed and Nitric Oxide-Mediated Relaxation Is Preserved in Retrieved Human Aortocoronary Vein Grafts",
abstract = "Although little is known about the endothelial cell function of human saphenous vein coronary artery bypass grafts, there is evidence to suggest that receptor-activated, endothelial-dependent relaxation mediated by nitric oxide is impaired. This study examines the expression and function of endothelial cell constitutive nitric oxide synthase (cNOS) of aortocoronary vein bypass grafts and human saphenous veins obtained from 10 patients undergoing repeat coronary artery bypass grafting for recurrent ischemic symptoms. Following precontraction with norepinephrine (10-5 M), responses to acetylcholine (receptor-mediated, endothelium-dependent), calcium ionophore (A23187; receptor-independent, endothelium-dependent), and sodium nitroprusside (endothelium-independent) were assessed. Following total RNA extraction using phenol/guanidinium isothiocyanate from specimens of human saphenous vein and vein graft, a quantitative RNase Protection Assay (RPA) was performed using a cRNA riboprobe corresponding to a fragment of the human endothelial cell cNOS gone. Histologically, the vein grafts showed both intimal hyperplasia development and focal atherosclerosis formation compared to the saphenous veins. Scanning electron microscopy of the saphenous veins and the vein grafts showed an intact endothelium. Precontracted vein grafts did not relax in response to acetylcholine; in contrast, the saphenous vein relaxed in a dose-dependent manner to reach a maximal relaxation of 19 ± 4% precontracted tension. Saphenous veins and vein grafts relaxed in response to A23187 with maximal relaxation of 92 ± 5 and 73 ± 13%, respectively. Both vessels relaxed in a dose dependent manner to sodium nitroprusside. RPA normalized to β-actin showed similar levels of expression of endothelial cell cNOS equivalent to 1 pg of sense RNA in both the saphenous vein and vein graft. These data demonstrate that the endothelium of coronary artery vein grafts shows both expression and function of endothelial cell cNOS. However, the impaired NO-mediated relaxation of aortocoronary vein grafts in response to acetylcholine appears to be due to altered receptor activation of the endothelial cNOS system.",
author = "Davies, {Mark G.} and Berkowitz, {Dan E.} and Hagen, {Per Otto}",
doi = "10.1006/jsre.1995.1116",
journal = "Journal of Surgical Research",
publisher = "Academic Press",
T1 - Constitutive Nitric Oxide Synthase Is Expressed and Nitric Oxide-Mediated Relaxation Is Preserved in Retrieved Human Aortocoronary Vein Grafts
AU - Davies, Mark G.
AU - Berkowitz, Dan E.
AU - Hagen, Per Otto
N2 - Although little is known about the endothelial cell function of human saphenous vein coronary artery bypass grafts, there is evidence to suggest that receptor-activated, endothelial-dependent relaxation mediated by nitric oxide is impaired. This study examines the expression and function of endothelial cell constitutive nitric oxide synthase (cNOS) of aortocoronary vein bypass grafts and human saphenous veins obtained from 10 patients undergoing repeat coronary artery bypass grafting for recurrent ischemic symptoms. Following precontraction with norepinephrine (10-5 M), responses to acetylcholine (receptor-mediated, endothelium-dependent), calcium ionophore (A23187; receptor-independent, endothelium-dependent), and sodium nitroprusside (endothelium-independent) were assessed. Following total RNA extraction using phenol/guanidinium isothiocyanate from specimens of human saphenous vein and vein graft, a quantitative RNase Protection Assay (RPA) was performed using a cRNA riboprobe corresponding to a fragment of the human endothelial cell cNOS gone. Histologically, the vein grafts showed both intimal hyperplasia development and focal atherosclerosis formation compared to the saphenous veins. Scanning electron microscopy of the saphenous veins and the vein grafts showed an intact endothelium. Precontracted vein grafts did not relax in response to acetylcholine; in contrast, the saphenous vein relaxed in a dose-dependent manner to reach a maximal relaxation of 19 ± 4% precontracted tension. Saphenous veins and vein grafts relaxed in response to A23187 with maximal relaxation of 92 ± 5 and 73 ± 13%, respectively. Both vessels relaxed in a dose dependent manner to sodium nitroprusside. RPA normalized to β-actin showed similar levels of expression of endothelial cell cNOS equivalent to 1 pg of sense RNA in both the saphenous vein and vein graft. These data demonstrate that the endothelium of coronary artery vein grafts shows both expression and function of endothelial cell cNOS. However, the impaired NO-mediated relaxation of aortocoronary vein grafts in response to acetylcholine appears to be due to altered receptor activation of the endothelial cNOS system.
AB - Although little is known about the endothelial cell function of human saphenous vein coronary artery bypass grafts, there is evidence to suggest that receptor-activated, endothelial-dependent relaxation mediated by nitric oxide is impaired. This study examines the expression and function of endothelial cell constitutive nitric oxide synthase (cNOS) of aortocoronary vein bypass grafts and human saphenous veins obtained from 10 patients undergoing repeat coronary artery bypass grafting for recurrent ischemic symptoms. Following precontraction with norepinephrine (10-5 M), responses to acetylcholine (receptor-mediated, endothelium-dependent), calcium ionophore (A23187; receptor-independent, endothelium-dependent), and sodium nitroprusside (endothelium-independent) were assessed. Following total RNA extraction using phenol/guanidinium isothiocyanate from specimens of human saphenous vein and vein graft, a quantitative RNase Protection Assay (RPA) was performed using a cRNA riboprobe corresponding to a fragment of the human endothelial cell cNOS gone. Histologically, the vein grafts showed both intimal hyperplasia development and focal atherosclerosis formation compared to the saphenous veins. Scanning electron microscopy of the saphenous veins and the vein grafts showed an intact endothelium. Precontracted vein grafts did not relax in response to acetylcholine; in contrast, the saphenous vein relaxed in a dose-dependent manner to reach a maximal relaxation of 19 ± 4% precontracted tension. Saphenous veins and vein grafts relaxed in response to A23187 with maximal relaxation of 92 ± 5 and 73 ± 13%, respectively. Both vessels relaxed in a dose dependent manner to sodium nitroprusside. RPA normalized to β-actin showed similar levels of expression of endothelial cell cNOS equivalent to 1 pg of sense RNA in both the saphenous vein and vein graft. These data demonstrate that the endothelium of coronary artery vein grafts shows both expression and function of endothelial cell cNOS. However, the impaired NO-mediated relaxation of aortocoronary vein grafts in response to acetylcholine appears to be due to altered receptor activation of the endothelial cNOS system.
U2 - 10.1006/jsre.1995.1116
DO - 10.1006/jsre.1995.1116
JO - Journal of Surgical Research
JF - Journal of Surgical Research