The Presto™ Plasmid 96 Well Binding Plate provides high-throughput purification of plasmid DNA from 1-5 ml bacterial cultures. The glass fiber membrane is optimized for binding plasmid DNA. The red O-ring stabilizes the white membrane during high-speed centrifugation and the column tip ensures uninhibited flow-through while eliminating the risk of cross contamination of adjacent wells. With plasmid DNA yields of up to 35 µg from a broad plasmid size range (1-15 kb), Presto™ Plasmid 96 Well Binding Plates are the high performance and cost effective solution for large scale plasmid DNA purification.
The quality of the Presto™ Plasmid 96 Well Binding Plate is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 ml overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 3.7 U/ml). Following the purification process, a yield of more than 20 μg per well is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.