The QIAamp MinElute Virus Spin Kit simplifies purification of viral DNA and RNA with fast spin-column procedures. The QIAamp MinElute Virus Spin Kit uses starting sample volumes of up to 0.2 ml and combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 μl. The QIAamp MinElute Virus Spin process can be fully automated on the QIAcube Connect.
The QIAamp MinElute Virus Spin Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
QIAamp MinElute Virus Spin procedure.
The percentage of positive samples at low virus titers is shown. Nucleic acids from an RNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subject to RT-PCR. For the spin procedure, 95% probit values for 32 samples were 22.84 IU/ml and for the vacuum procedure, they were 9.46 IU/ml.
Purified viral nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications such as PCR and RT-PCR (see figures "High sensitivity in PCR" and "High sensitivity in RT-PCR").
The QIAamp MinElute Virus Spin Kit simplifies isolation of viral RNA and DNA from plasma, serum, and cell-free body fluids with a fast spin-column procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp MinElute technology yields viral RNA and DNA from plasma, serum, and cell-free body fluids ready to use in PCR and blotting procedures.
The QIAamp MinElute Spin Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. The procedure is suitable for use with plasma, serum, and other cell-free body fluids. QIAamp sample preparation technology is fully licensed.
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp MinElute membrane (see flowchart "QIAamp MinElute Virus Spin procedure"). Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and viral nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases, and other impurities.
What is the difference between the QIAamp MinElute Virus Spin and the MinElute Virus Vacuum Kit?
Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?
Is QIAGEN carrier RNA sold separately?