RNase Inhibitor, Human Placenta, specifically inhibits RNases A, B and C.
RNase Inhibitor, Human Placenta is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C (1). It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor, Human Placenta is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).
The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014 (2).
An E. coli strain that carries the Ribonuclease Inhibitor gene from human placenta.
One unit is defined as the amount of RNase Inhibitor, Human Placenta required to inhibit the activity of 5 ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2, 3'-cyclic monophosphate by RNase A.
Blackburn, P. and Moore, S. (1982). Pancreatic Ribonucleases . The Enzymes. XV, Part B, Academic Press, NY.
Blackburn, P., Wilson, G. and Moore, S. (1977). J. Biol. Chem. 252,5904.