Our study is among the first to evaluate both PIK3CA mutations and PTEN protein expression status in trastuzumab-treated patients, and it confirms the results of previous investigators that these two markers are involved in the development of resistance to trastuzumab [5‚Äì12, 22, 23].
Sample text:```Intriguingly, using MPRs-deficient MEF cells, mouse and human CLN5 have been shown to reach lysosomes in an MPRindependent manner [12].```
Sample intent: background
Sample text:```Several studies have shown that a major function of PP2A is downregulation of the Ras/MAP kinase pathway (2, 3, 22, 30, 50, 51, 53).```
Sample intent: method



Sample text:```Interestingly, MHC I and ERAP1 were coordinately expressed in normal and, to a lesser extent, neoplastic cells and tumor lesions (13, 14).```
Sample intent: method



Sample text:```Our results show that after 4 h of thiotepa treatment, the level of expression of anti-apoptotic Bcl-2 was not changed; this confirms the results of a previous study [25].```
Sample intent: result



Sample text:```In addition, consistent with the result of our preclinical study, recent clinical study showed that alectinib was also highly active against ALK-positive NSCLC patients after treatment with crizotinib [21].```
Sample intent: result



Sample text:```In line with our results, several previous studies demonstrated the anti-proliferative effect of PPARs agonists in several cell types [11-15].```
Sample intent: result
Sample text:```Our results further confirm previous findings on the differential regulatory mechanisms for Hoxa3 and Hoxb3 expression in r5 and r6 by kreisler (Manzanares et al., 1997, 1999a,b, 2001).```
Sample intent: result



Sample text:```We used longitudinal sequence samples for 15 patients from two independent studies [13,22].```
Sample intent: background



Sample text:```We screened for potential TF binding sites using STAMP [59], with the motifs’ position weight matrices (PWMs) retrieved from the TRANSFAC database [60].```
Sample intent: background



Sample text:```This study supports the theory of a tumor origin from multipotential progenitor cells.(14) Reports of the Ki67 labeling index have demonstrated great variety.```
Sample intent: result



Sample text:```Interestingly, MHC I and ERAP1 were coordinately expressed in normal and, to a lesser extent, neoplastic cells and tumor lesions (13, 14).```
Sample intent: method



Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background



Sample text:```This means that accumulation of the CMG helicase with hyperphosphorylated Psf1 is a sensitive marker for the aberrant firing of late-origins in cells treated with hydroxyurea (42), reflecting a specific failure to activate the S-phase checkpoint response at some point downstream of the Mec1 kinase.```
Sample intent: method



Sample text:```They demonstrated by in vitro and ex vivo studies that ATP1A1 mutants showed loss of pump activity and strongly reduced affinity for potassium, causing inappropriate depolarization of the cells (Beuschlein et al. 2013).```
Sample intent: method



Sample text:```In addition, consistent with the result of our preclinical study, recent clinical study showed that alectinib was also highly active against ALK-positive NSCLC patients after treatment with crizotinib [21].```
Sample intent: result



Sample text:```Several studies have shown that a major function of PP2A is downregulation of the Ras/MAP kinase pathway (2, 3, 22, 30, 50, 51, 53).```
Sample intent: method