PCR amplification and DNA sequencing Eight loci including the partial rDNA-ITS (ITS), ac t in (ACT ) , ch i t in syn thase 1 (CHS-1 ) , glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-tubulin (TUB2), Histone (CYLH3), glutamine synthetase (GS) and ApMat were amplified and sequenced using the primer pairs ITS-1 F (Gardes and Bruns 1993) /ITS-4 (White et al.
Sample text:```This process is controlled not only by the protein-coding segments of the genome but also by non-coding regions, including loci that produce small RNAs (Grivna et al., 2006; He et al., 2009; Thomson and Lin, 2009).```
Sample intent: method
Sample text:```Previous studies have shown that sialome genes were subject to strong ancient selective pressures that have resulted in species-specific adaptations (Varki 2001; Angata et al. 2004; Altheide et al. 2006; Varki & Gagneux 2009), suggesting that they likely evolved in response to ancient pathogens The function of sialome genes, i.```
Sample intent: result



Sample text:```In the subsequent studies, Yoshida et al. (2004b) and Ishii et al. (2004) confirmed the reversibility of 2,6-dihydroxybenzoate decarboxylase of Rhizobium sp. strain MTP-10005 and R.radiobacter WU-0108, respectively.```
Sample intent: result



Sample text:```The vast majority of mutations in FUS/TLS-related familial ALS cases were identified in C-terminal NLS, which result in the retention and the inclusion of FUS/TLS in the cytoplasm [2,3,5,8,14], and FUS/TLS as well as TDP-43 is recruited to SGs under stress conditions such as arsenite, heat shock and hypoxia [28,29,30].```
Sample intent: method



Sample text:```3, top) was constructed from the previously described 0.85-kb promoter construct (construct 6, Drews et al. 2005) by digestion with AscI and MluI followed by religation.```
Sample intent: method



Sample text:```Amplicon preparation, sequencing and data analysis were conducted for all 44 samples as described previously (Hospodsky et al., 2012).```
Sample intent: background
Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background



Sample text:```A number of studies have been published recently, which analyze the location, orientation, and identity ofmacromolecular complexes, based on the outcome of template-matching (Brandt et al., 2009; Ortiz et al., 2010).```
Sample intent: method



Sample text:```This is interesting in relation to the result from our previous CGH study (comparing the number of significantly hybridizing probes on the microarray) that showed high sequence divergence on the Z chromosome between the common whitethroat and the zebra finch [62]; and such patterns are also found in other species [48,51,69].```
Sample intent: result



Sample text:```To further characterize isolates, random amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was performed using an adaptation of methods described elsewhere.(10)```
Sample intent: background



Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result



Sample text:```The vast majority of mutations in FUS/TLS-related familial ALS cases were identified in C-terminal NLS, which result in the retention and the inclusion of FUS/TLS in the cytoplasm [2,3,5,8,14], and FUS/TLS as well as TDP-43 is recruited to SGs under stress conditions such as arsenite, heat shock and hypoxia [28,29,30].```
Sample intent: method



Sample text:```Methods for extraction, amplification and sequencing of the nuclear 5.8S rRNA gene and the two flanking, ITS regions were as previously published (Ganley et al., 2004; Shipunov et al., 2008).```
Sample intent: background



Sample text:```In the subsequent studies, Yoshida et al. (2004b) and Ishii et al. (2004) confirmed the reversibility of 2,6-dihydroxybenzoate decarboxylase of Rhizobium sp. strain MTP-10005 and R.radiobacter WU-0108, respectively.```
Sample intent: result



Sample text:```Our genealogy, based on many alleles but only the first exon of bindin, is not entirely consistent with that of McCartney and Lessios (2004), based on fewer alleles but incorporating the entire molecule.```
Sample intent: result



Sample text:```This process is controlled not only by the protein-coding segments of the genome but also by non-coding regions, including loci that produce small RNAs (Grivna et al., 2006; He et al., 2009; Thomson and Lin, 2009).```
Sample intent: method