Expecting that, like HCV infection, hepacivirus infection in animals might persist (30), we developed two broadly reactive PCR assays targeting highly conserved sequence motifs in CHV 5= UTR and helicase to detect the genetically related hepacivirus genomic RNA in serum samples of horses and cows.
Sample text:```Mutation sites in the receptor-binding domain of the spike protein The spike protein of CoVs is responsible for cell receptor attachment and membrane fusion to mediate virus entry (Li, 2015).```
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Sample text:```Our results further confirm previous findings on the differential regulatory mechanisms for Hoxa3 and Hoxb3 expression in r5 and r6 by kreisler (Manzanares et al., 1997, 1999a,b, 2001).```
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Sample text:```Cell-tracing experiments in chicken (Jaffredo et al., 2000; Jaffredo et al., 1998) and mouse (Chen et al., 2009; de Bruijn et al., 2002; Sugiyama et al., 2003; Zovein et al., 2008) species indicate that IAHCs derive from hemogenic ECs through an Endothelial-to-Hematopoietic Transition (EHT).```
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Sample text:```Methods for extraction, amplification and sequencing of the nuclear 5.8S rRNA gene and the two flanking, ITS regions were as previously published (Ganley et al., 2004; Shipunov et al., 2008).```
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Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
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Sample text:```Amplicon preparation, sequencing and data analysis were conducted for all 44 samples as described previously (Hospodsky et al., 2012).```
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Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
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Sample text:```In our previous study, we found that RAG-mediated nicking and cleavage of oligonucleotide substrates containing cRSSs identified from lymphoid malignancies were either unchanged or slightly reduced in the presence of HMGB1 for most cRSSs tested (including LMO2, TAL1, Hox11, SIL, SCL), except Ttg-1 [24].```
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Sample text:```This means that accumulation of the CMG helicase with hyperphosphorylated Psf1 is a sensitive marker for the aberrant firing of late-origins in cells treated with hydroxyurea (42), reflecting a specific failure to activate the S-phase checkpoint response at some point downstream of the Mec1 kinase.```
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Sample text:```Cell-tracing experiments in chicken (Jaffredo et al., 2000; Jaffredo et al., 1998) and mouse (Chen et al., 2009; de Bruijn et al., 2002; Sugiyama et al., 2003; Zovein et al., 2008) species indicate that IAHCs derive from hemogenic ECs through an Endothelial-to-Hematopoietic Transition (EHT).```
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Sample text:```Our results confi rm previous studies that reached the same conclusion (Boushey et al 1994; Vermeulen et al 2000; Till et al 2005; Marcucci et al 2005).```
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Sample text:```Amplicon preparation, sequencing and data analysis were conducted for all 44 samples as described previously (Hospodsky et al., 2012).```
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Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
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Sample text:```This is interesting in relation to the result from our previous CGH study (comparing the number of significantly hybridizing probes on the microarray) that showed high sequence divergence on the Z chromosome between the common whitethroat and the zebra finch [62]; and such patterns are also found in other species [48,51,69].```
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Sample text:```Our results further confirm previous findings on the differential regulatory mechanisms for Hoxa3 and Hoxb3 expression in r5 and r6 by kreisler (Manzanares et al., 1997, 1999a,b, 2001).```
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Sample text:```To fulfill these functions, Hsp90 interacts with its cofactors and cochaperones including Hsp70, immunophilins, and p23, to form the Hsp90based chaperone complex.(1,2) Natural compounds such as geldanamycin and radicicol bind the ATP-binding pocket of Hsp90, and disrupt its chaperone function.```
Sample intent: method