Once attached, it is believed that retraction of the pilus moves one or more F6 particles in close proximity to the bacterial cell membrane, allowing membrane fusion [12] and ultimately phage entry into the cell via the viral lytic enzyme P5 [10,13].
Sample text:```Intriguingly, using MPRs-deficient MEF cells, mouse and human CLN5 have been shown to reach lysosomes in an MPRindependent manner [12].```
Sample intent: background
Sample text:```Mutation sites in the receptor-binding domain of the spike protein The spike protein of CoVs is responsible for cell receptor attachment and membrane fusion to mediate virus entry (Li, 2015).```
Sample intent: method



Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background



Sample text:```MtrF has been proposed either to shuttle antimicrobials to the pump for export or to help stabilize one or more components of the efflux pump (56, 57).```
Sample intent: method



Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background



Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result
Sample text:```Intriguingly, using MPRs-deficient MEF cells, mouse and human CLN5 have been shown to reach lysosomes in an MPRindependent manner [12].```
Sample intent: background



Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
Sample intent: background



Sample text:```…is bifunctional: it contributes to FS engulfment and it forms a feeding channel that allows nutrients (and possibly regulatory signals) to be transferred from the MC to the FS during spore assembly (Broder and Pogliano, 2006; Camp and Losick, 2009; Doan et al., 2009; Higgins and Dworkin, 2012).```
Sample intent: method



Sample text:```Our genealogy, based on many alleles but only the first exon of bindin, is not entirely consistent with that of McCartney and Lessios (2004), based on fewer alleles but incorporating the entire molecule.```
Sample intent: result



Sample text:```It is clear from these results and those from previous studies Bagwell et al. 1998; 2001; Bergholz et al. 2001 that numerous diazotrophs inhabiting the rhizoplanes of salt marsh plants are strongly influenced by specific properties of the plant host and somewhat less influenced by the abiotic…```
Sample intent: result



Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background



Sample text:```This discovery, along with the fact that both intrinsic and Rho-dependent terminators have associated pausing, suggested that antipausing can underlie antitermination, which is at least partly true (126).```
Sample intent: method



Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result



Sample text:```Previous studies have shown that sialome genes were subject to strong ancient selective pressures that have resulted in species-specific adaptations (Varki 2001; Angata et al. 2004; Altheide et al. 2006; Varki & Gagneux 2009), suggesting that they likely evolved in response to ancient pathogens The function of sialome genes, i.```
Sample intent: result



Sample text:```Methods for extraction, amplification and sequencing of the nuclear 5.8S rRNA gene and the two flanking, ITS regions were as previously published (Ganley et al., 2004; Shipunov et al., 2008).```
Sample intent: background