Nitrosoguanidine mutagenesis and isolation of mutants
The mutagenesis process was carried out as described previously (Eslava et al. 1975).
Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background
Sample text:```The sgl gene has been shown to be expressed differentially during development (Binari et al. 1997; Haerry et al. 1997).```
Sample intent: method



Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background



Sample text:```In our previous study, we found that RAG-mediated nicking and cleavage of oligonucleotide substrates containing cRSSs identified from lymphoid malignancies were either unchanged or slightly reduced in the presence of HMGB1 for most cRSSs tested (including LMO2, TAL1, Hox11, SIL, SCL), except Ttg-1 [24].```
Sample intent: method



Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result



Sample text:```Our results further confirm previous findings on the differential regulatory mechanisms for Hoxa3 and Hoxb3 expression in r5 and r6 by kreisler (Manzanares et al., 1997, 1999a,b, 2001).```
Sample intent: result
Sample text:```Our results further confirm previous findings on the differential regulatory mechanisms for Hoxa3 and Hoxb3 expression in r5 and r6 by kreisler (Manzanares et al., 1997, 1999a,b, 2001).```
Sample intent: result



Sample text:```In our previous study, we found that RAG-mediated nicking and cleavage of oligonucleotide substrates containing cRSSs identified from lymphoid malignancies were either unchanged or slightly reduced in the presence of HMGB1 for most cRSSs tested (including LMO2, TAL1, Hox11, SIL, SCL), except Ttg-1 [24].```
Sample intent: method



Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
Sample intent: background



Sample text:```Methods for extraction, amplification and sequencing of the nuclear 5.8S rRNA gene and the two flanking, ITS regions were as previously published (Ganley et al., 2004; Shipunov et al., 2008).```
Sample intent: background



Sample text:```A linear relationship between erythrocyte GSH-Px and whole blood Se concentration was already described in camel [3, 8].```
Sample intent: method



Sample text:```3, top) was constructed from the previously described 0.85-kb promoter construct (construct 6, Drews et al. 2005) by digestion with AscI and MluI followed by religation.```
Sample intent: method



Sample text:```germination stimulatory activity by germination bioassays with Striga hermonthica as previously described by Jamil et al. (2010).```
Sample intent: background



Sample text:```When MgTx was applied to the rare cells subsequently shown to express only TTX-sensitive Na current (A-type neurons) (Stansfeld and Wallis 1985; Schild and Kunze 1997; Li and Schild 2007b), there was no change in the duration of the action potential measured at 0 mV (0.74 0.09 vs. 0.76 0.1 ms, n 3,…```
Sample intent: method



Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result



Sample text:```This study supports the theory of a tumor origin from multipotential progenitor cells.(14) Reports of the Ki67 labeling index have demonstrated great variety.```
Sample intent: result