To study the development of the human mammary ductal system in vitro, we adapted procedures used for the culture of primary mouse mammary organoids (Hirai et al. 1998).
Sample text:```Endothelial cell cultures and capillary tubeswere generated as previously described (5, 11).```
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Sample text:```…proteins initially has been demonstrated in tissue culture model systems of adipogenesis and hematopoiesis (Lin and Lane 1992; Freytag et al. 1994; Hu et al. 1995; Müller et al. 1995; Nerlov et al. 1998) and has now been firmly established through analysis of the respective knockout mice…```
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Sample text:```This study supports the theory of a tumor origin from multipotential progenitor cells.(14) Reports of the Ki67 labeling index have demonstrated great variety.```
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Sample text:```These results are in line with morphological abnormalities in a rabbit model of pneumococcal meningitis [8, 47], in neonatal and adult rat models [7, 51] and in human autopsy cases [20].```
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Sample text:```Isolation of cardiomyocytes by standard enzymatic techniques and patch-clamp recordings were performed as described previously [42].```
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Sample text:```Interestingly, MHC I and ERAP1 were coordinately expressed in normal and, to a lesser extent, neoplastic cells and tumor lesions (13, 14).```
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Sample text:```The sgl gene has been shown to be expressed differentially during development (Binari et al. 1997; Haerry et al. 1997).```
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Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
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Sample text:```Isolation of cardiomyocytes by standard enzymatic techniques and patch-clamp recordings were performed as described previously [42].```
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Sample text:```Previous studies described that ectopic miR-99a expression slowed keratinocyte growth and liver cancer cell growth by blocking the cell cycle at the G1/S transition [10,29].```
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Sample text:```Our heritability result is generally in agreement with the studies of Kien et al. (2008) in Eucalyptus urophylla and Zhao et al. (2014a) in poplar.```
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Sample text:```Interestingly, MHC I and ERAP1 were coordinately expressed in normal and, to a lesser extent, neoplastic cells and tumor lesions (13, 14).```
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Sample text:```These results are in line with morphological abnormalities in a rabbit model of pneumococcal meningitis [8, 47], in neonatal and adult rat models [7, 51] and in human autopsy cases [20].```
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Sample text:```Our results further confirm previous findings on the differential regulatory mechanisms for Hoxa3 and Hoxb3 expression in r5 and r6 by kreisler (Manzanares et al., 1997, 1999a,b, 2001).```
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Sample text:```Intriguingly, using MPRs-deficient MEF cells, mouse and human CLN5 have been shown to reach lysosomes in an MPRindependent manner [12].```
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Sample text:```In the first pass, we display the diffuse lobe of the BRDF through spherical harmonic environment map rendering [Ramamoorthi and Hanrahan 2001].```
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