Detection of anti-eye muscle membrane (EyeM) and cytosol (EyeC) antibodies: Antibodies against eye muscle membrane (EyeM) and cytosol (EyeC) fractions were measured by enzyme-linked immunosorbent assay (ELISA) detailed in our previous paper [14].
Sample text:```Intriguingly, using MPRs-deficient MEF cells, mouse and human CLN5 have been shown to reach lysosomes in an MPRindependent manner [12].```
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Sample text:```, 2009), are most abundant on endosomes undergoing the early-to-late transition (Rojas et al., 2008; Cullen and Korswagen, 2012; van Weering et al., 2012).```
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Sample text:```Endothelial cell cultures and capillary tubeswere generated as previously described (5, 11).```
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Sample text:```In line with previous results (3, 19, 20, 30), we found that during muscle contraction ERK1/2 phosphorylation was increased and was not affected by CaMKII inhibition.```
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Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
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Sample text:```Intriguingly, using MPRs-deficient MEF cells, mouse and human CLN5 have been shown to reach lysosomes in an MPRindependent manner [12].```
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Sample text:```Disruption of oculomotor motoneuron maturation alters the molecular profile of EOM, including suppression of Myh13 (5, 7, 11).```
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Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
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Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
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Sample text:```In line with previous results (3, 19, 20, 30), we found that during muscle contraction ERK1/2 phosphorylation was increased and was not affected by CaMKII inhibition.```
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Sample text:```Previous studies revealed that acylcarnitines were elevated early after APAP treatment and that their elevation was indicative of mitochondrial damage and dysfunction.(15,19) In the present study these observations were confirmed, as palmitoylcarnitine was elevated by toxic doses of APAP and maintained at normal levels (compared with untreated controls) by Wy-14,643 pretreatment.```
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Sample text:```Our results confi rm previous studies that reached the same conclusion (Boushey et al 1994; Vermeulen et al 2000; Till et al 2005; Marcucci et al 2005).```
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Sample text:```Endothelial cell cultures and capillary tubeswere generated as previously described (5, 11).```
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Sample text:```Isolation of cardiomyocytes by standard enzymatic techniques and patch-clamp recordings were performed as described previously [42].```
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Sample text:```Other studies suggest that altering affinity to FcRn might alter the bioavailability of mAbs (Deng et al., 2010; Deng et al., 2012), and SC bioavailability appears lower in FcRn-deficient mice as compared to wild type animals (Wang et al., 2008).```
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Sample text:```Our genealogy, based on many alleles but only the first exon of bindin, is not entirely consistent with that of McCartney and Lessios (2004), based on fewer alleles but incorporating the entire molecule.```
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