A fourth and final C-terminal helix, extending from residue 184 to the C-terminus, forms a long domainswapped helix that is significantly kinked at residues 211– 213 and is packed against its symmetry mate, forming an amphipathic two-helix bundle structure and inter-subunit contacts that closely resemble an antiparallel coiled-coil peptide fold (23) (Figure 2B).
Sample text:```U-box domain does not depend on zinc binding to keep its stability, but have evolved instead networks of hydrogen bonds and salt bridges formed by the corresponding residues at metal ligand positions (Hatakeyama et al., 2001; Nordquist et al., 2010; Wiborg et al., 2008).```
Sample intent: method
Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background



Sample text:```Our genealogy, based on many alleles but only the first exon of bindin, is not entirely consistent with that of McCartney and Lessios (2004), based on fewer alleles but incorporating the entire molecule.```
Sample intent: result



Sample text:```Extension of protein annotation We used BacProt (publication in progress, see [33] for details) to complement the present annotation of protein-coding genes for each Aquificales genome.```
Sample intent: background



Sample text:```We screened for potential TF binding sites using STAMP [59], with the motifs’ position weight matrices (PWMs) retrieved from the TRANSFAC database [60].```
Sample intent: background



Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
Sample intent: background
Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background



Sample text:```7C) that may correspond to the centrosomal katanin previously observed after nocodazole-mediated disruption of the spindle pole (McNally and Thomas, 1998).```
Sample intent: method



Sample text:```U-box domain does not depend on zinc binding to keep its stability, but have evolved instead networks of hydrogen bonds and salt bridges formed by the corresponding residues at metal ligand positions (Hatakeyama et al., 2001; Nordquist et al., 2010; Wiborg et al., 2008).```
Sample intent: method



Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
Sample intent: background



Sample text:```We screened for potential TF binding sites using STAMP [59], with the motifs’ position weight matrices (PWMs) retrieved from the TRANSFAC database [60].```
Sample intent: background



Sample text:```To fulfill these functions, Hsp90 interacts with its cofactors and cochaperones including Hsp70, immunophilins, and p23, to form the Hsp90based chaperone complex.(1,2) Natural compounds such as geldanamycin and radicicol bind the ATP-binding pocket of Hsp90, and disrupt its chaperone function.```
Sample intent: method



Sample text:```Extension of protein annotation We used BacProt (publication in progress, see [33] for details) to complement the present annotation of protein-coding genes for each Aquificales genome.```
Sample intent: background



Sample text:```Our results show that after 4 h of thiotepa treatment, the level of expression of anti-apoptotic Bcl-2 was not changed; this confirms the results of a previous study [25].```
Sample intent: result



Sample text:```This discovery, along with the fact that both intrinsic and Rho-dependent terminators have associated pausing, suggested that antipausing can underlie antitermination, which is at least partly true (126).```
Sample intent: method



Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background