For experiments studying FMT (44), fibroblasts were cultured in DMEM/F12 10% FBS at low density for 24 hours, then at 40%–50% confluency, media was changed to DMEM/F12 1% FBS for 4 hours prior to treatments with SMOC2 (Preprotech) and/or TGFβ1(Preprotech).
Sample text:```ly), which have been originally identified as molecules activated by growth factor-stimulation of fibroblusts, are key kinases in the intracellular signal transduction pathways (1-6).```
Sample intent: method
Sample text:```ly), which have been originally identified as molecules activated by growth factor-stimulation of fibroblusts, are key kinases in the intracellular signal transduction pathways (1-6).```
Sample intent: method



Sample text:```Our results show that after 4 h of thiotepa treatment, the level of expression of anti-apoptotic Bcl-2 was not changed; this confirms the results of a previous study [25].```
Sample intent: result



Sample text:```Isolation of cardiomyocytes by standard enzymatic techniques and patch-clamp recordings were performed as described previously [42].```
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Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
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Sample text:```In line with our results, several previous studies demonstrated the anti-proliferative effect of PPARs agonists in several cell types [11-15].```
Sample intent: result
Sample text:```Isolation of cardiomyocytes by standard enzymatic techniques and patch-clamp recordings were performed as described previously [42].```
Sample intent: background



Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
Sample intent: background



Sample text:```Endothelial cell cultures and capillary tubeswere generated as previously described (5, 11).```
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Sample text:```Other studies suggest that altering affinity to FcRn might alter the bioavailability of mAbs (Deng et al., 2010; Deng et al., 2012), and SC bioavailability appears lower in FcRn-deficient mice as compared to wild type animals (Wang et al., 2008).```
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Sample text:```In line with our results, several previous studies demonstrated the anti-proliferative effect of PPARs agonists in several cell types [11-15].```
Sample intent: result



Sample text:```Our results show that after 4 h of thiotepa treatment, the level of expression of anti-apoptotic Bcl-2 was not changed; this confirms the results of a previous study [25].```
Sample intent: result



Sample text:```Basal ATP concentrations in the bulk medium of unstimulated CFs are low, but the concentrations of released nucleotides near the cell surface may be sufficient to activate adjacent P2Y receptors and contribute to basal signaling (48, 49).```
Sample intent: method



Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
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Sample text:```Group 3: mice were anesthetized in a Plexiglas chamber with 3% isoflurane in air for 25 min (after the desired concentration was reached) twice daily, as described previously (12); 0.```
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Sample text:```This study supports the theory of a tumor origin from multipotential progenitor cells.(14) Reports of the Ki67 labeling index have demonstrated great variety.```
Sample intent: result