The free energy of unfolding increased 5.6 kcal/mol in the presence of sodium citrate, which is similar to the increase in DG of unfolding of Ribonuclease A in the presence of 200 mM sodium sulfate (Ramos and Baldwin, 2002).
Sample text:```In line with previous results (3, 19, 20, 30), we found that during muscle contraction ERK1/2 phosphorylation was increased and was not affected by CaMKII inhibition.```
Sample intent: result
Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result



Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background



Sample text:```germination stimulatory activity by germination bioassays with Striga hermonthica as previously described by Jamil et al. (2010).```
Sample intent: background



Sample text:```U-box domain does not depend on zinc binding to keep its stability, but have evolved instead networks of hydrogen bonds and salt bridges formed by the corresponding residues at metal ligand positions (Hatakeyama et al., 2001; Nordquist et al., 2010; Wiborg et al., 2008).```
Sample intent: method



Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background
Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background



Sample text:```This means that accumulation of the CMG helicase with hyperphosphorylated Psf1 is a sensitive marker for the aberrant firing of late-origins in cells treated with hydroxyurea (42), reflecting a specific failure to activate the S-phase checkpoint response at some point downstream of the Mec1 kinase.```
Sample intent: method



Sample text:```In the subsequent studies, Yoshida et al. (2004b) and Ishii et al. (2004) confirmed the reversibility of 2,6-dihydroxybenzoate decarboxylase of Rhizobium sp. strain MTP-10005 and R.radiobacter WU-0108, respectively.```
Sample intent: result



Sample text:```Our results confi rm previous studies that reached the same conclusion (Boushey et al 1994; Vermeulen et al 2000; Till et al 2005; Marcucci et al 2005).```
Sample intent: result



Sample text:```In our previous study, we found that RAG-mediated nicking and cleavage of oligonucleotide substrates containing cRSSs identified from lymphoid malignancies were either unchanged or slightly reduced in the presence of HMGB1 for most cRSSs tested (including LMO2, TAL1, Hox11, SIL, SCL), except Ttg-1 [24].```
Sample intent: method



Sample text:```Methods for extraction, amplification and sequencing of the nuclear 5.8S rRNA gene and the two flanking, ITS regions were as previously published (Ganley et al., 2004; Shipunov et al., 2008).```
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Sample text:```Discrepancies analogous to those between our results and those of simulations have, indeed, recently been observed in the case of the binary perceptron [18], the pspin interaction spin glass [19], and in the case of fluctuating manifolds in random media [20], where dynamically frozen phases were observed in regions of parameter space in which static approaches yielded ergodic phases.```
Sample intent: result



Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
Sample intent: background



Sample text:```U-box domain does not depend on zinc binding to keep its stability, but have evolved instead networks of hydrogen bonds and salt bridges formed by the corresponding residues at metal ligand positions (Hatakeyama et al., 2001; Nordquist et al., 2010; Wiborg et al., 2008).```
Sample intent: method



Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background