To verify the specificity of our mass spectrometry protein identification, interactions with the known effectors RILP and GDI2 and the novel interactors VapB, SPG21, PHB, and stomatin-like 2 were confirmed by coimmunoprecipitation followed by Western blotting (Supplementary Fig.
Sample text:```We screened for potential TF binding sites using STAMP [59], with the motifs’ position weight matrices (PWMs) retrieved from the TRANSFAC database [60].```
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Sample text:```We screened for potential TF binding sites using STAMP [59], with the motifs’ position weight matrices (PWMs) retrieved from the TRANSFAC database [60].```
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Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
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Sample text:```Our genealogy, based on many alleles but only the first exon of bindin, is not entirely consistent with that of McCartney and Lessios (2004), based on fewer alleles but incorporating the entire molecule.```
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Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
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Sample text:```In line with our results, several previous studies demonstrated the anti-proliferative effect of PPARs agonists in several cell types [11-15].```
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Sample text:```germination stimulatory activity by germination bioassays with Striga hermonthica as previously described by Jamil et al. (2010).```
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Sample text:```To fulfill these functions, Hsp90 interacts with its cofactors and cochaperones including Hsp70, immunophilins, and p23, to form the Hsp90based chaperone complex.(1,2) Natural compounds such as geldanamycin and radicicol bind the ATP-binding pocket of Hsp90, and disrupt its chaperone function.```
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Sample text:```In line with our results, several previous studies demonstrated the anti-proliferative effect of PPARs agonists in several cell types [11-15].```
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Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
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Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
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Sample text:```1996) and then to the effector enzyme, namely phospholipase C-b 2 (PLC-b 2) (Adler et al. 2000).```
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Sample text:```This means that accumulation of the CMG helicase with hyperphosphorylated Psf1 is a sensitive marker for the aberrant firing of late-origins in cells treated with hydroxyurea (42), reflecting a specific failure to activate the S-phase checkpoint response at some point downstream of the Mec1 kinase.```
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Sample text:```Our results further confirm previous findings on the differential regulatory mechanisms for Hoxa3 and Hoxb3 expression in r5 and r6 by kreisler (Manzanares et al., 1997, 1999a,b, 2001).```
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Sample text:```The extracellular region of SIRPα interacts with its ligand, CD47, which is also a member of the Ig superfamily,(19,20,24) with such interaction promoting the tyrosine phosphorylation of SIRPα.(20,25) In contrast to the relatively restricted distribution of SIRPα, CD47 is expressed in most cell types including a variety of hematopoietic cells.```
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Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
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