All insertions consist of direct repeats of the DNA sequence, which are removed precisely, presumably by recombination or ‘slippage’ during DNA replication or repair (Farabaugh et al., 1978; Albertini et al., 1982).
Sample text:```Because most off-target nicks are not adjacent to each other and do not form DSBs, these nicks are repaired precisely and rarely lead to indels (Ran et al. 2013a; Shen et al. 2014).```
Sample intent: method
Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result



Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
Sample intent: background



Sample text:```3, top) was constructed from the previously described 0.85-kb promoter construct (construct 6, Drews et al. 2005) by digestion with AscI and MluI followed by religation.```
Sample intent: method



Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background



Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background
Sample text:```This discovery, along with the fact that both intrinsic and Rho-dependent terminators have associated pausing, suggested that antipausing can underlie antitermination, which is at least partly true (126).```
Sample intent: method



Sample text:```This is interesting in relation to the result from our previous CGH study (comparing the number of significantly hybridizing probes on the microarray) that showed high sequence divergence on the Z chromosome between the common whitethroat and the zebra finch [62]; and such patterns are also found in other species [48,51,69].```
Sample intent: result



Sample text:```Previous studies have shown that sialome genes were subject to strong ancient selective pressures that have resulted in species-specific adaptations (Varki 2001; Angata et al. 2004; Altheide et al. 2006; Varki & Gagneux 2009), suggesting that they likely evolved in response to ancient pathogens The function of sialome genes, i.```
Sample intent: result



Sample text:```Because most off-target nicks are not adjacent to each other and do not form DSBs, these nicks are repaired precisely and rarely lead to indels (Ran et al. 2013a; Shen et al. 2014).```
Sample intent: method



Sample text:```Therefore, one manifestation of the BRCA1–TONSL genetic interaction may well operate at sites of UV-induced damage, where both proteins are normally engaged in complex DNA damage responses (Duro et al. 2010; O’Connell et al. 2010; O’Donnell et al. 2010; Piwko et al. 2010; Pathania et al. 2011).```
Sample intent: method



Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background



Sample text:```Our genealogy, based on many alleles but only the first exon of bindin, is not entirely consistent with that of McCartney and Lessios (2004), based on fewer alleles but incorporating the entire molecule.```
Sample intent: result



Sample text:```To further characterize isolates, random amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was performed using an adaptation of methods described elsewhere.(10)```
Sample intent: background



Sample text:```This process is controlled not only by the protein-coding segments of the genome but also by non-coding regions, including loci that produce small RNAs (Grivna et al., 2006; He et al., 2009; Thomson and Lin, 2009).```
Sample intent: method



Sample text:```Amplicon preparation, sequencing and data analysis were conducted for all 44 samples as described previously (Hospodsky et al., 2012).```
Sample intent: background