The nuclear-targeted cameleon calcium sensor (Sieberer et al., 2009) was excitedwith the argon ion 458-nm laser and imaged using emission filters 476 to 486 nm for CFP and 529 to 540 nm for YFP.
Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result
Sample text:```In line with our results, several previous studies demonstrated the anti-proliferative effect of PPARs agonists in several cell types [11-15].```
Sample intent: result



Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result



Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background



Sample text:```The sequence of intermediates in the hR photocycle and their interconversions have been the subject of numerous studies (Va´ró et al., 1995a, b; Hazemoto et al., 1984; Zimányi and Lanyi, 1989a, b; Zima´nyi et al., 1989; Spencer and Dewey, 1990; Ames et al., 1992; Dioumaev and Braiman, 1997a).```
Sample intent: method



Sample text:```In this case, FLNA(16-23) showed more rapid association and slower dissociation rates to the β-arr2/ICL3 complex than to ICL3 alone (Figure 7D), suggesting that the FLNA fragment interacts with β-arr2 rather than with ICL3.```
Sample intent: method
Sample text:```Our results further confirm previous findings on the differential regulatory mechanisms for Hoxa3 and Hoxb3 expression in r5 and r6 by kreisler (Manzanares et al., 1997, 1999a,b, 2001).```
Sample intent: result



Sample text:```The sequence of intermediates in the hR photocycle and their interconversions have been the subject of numerous studies (Va´ró et al., 1995a, b; Hazemoto et al., 1984; Zimányi and Lanyi, 1989a, b; Zima´nyi et al., 1989; Spencer and Dewey, 1990; Ames et al., 1992; Dioumaev and Braiman, 1997a).```
Sample intent: method



Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background



Sample text:```The extracellular region of SIRPα interacts with its ligand, CD47, which is also a member of the Ig superfamily,(19,20,24) with such interaction promoting the tyrosine phosphorylation of SIRPα.(20,25) In contrast to the relatively restricted distribution of SIRPα, CD47 is expressed in most cell types including a variety of hematopoietic cells.```
Sample intent: method



Sample text:```Intriguingly, using MPRs-deficient MEF cells, mouse and human CLN5 have been shown to reach lysosomes in an MPRindependent manner [12].```
Sample intent: background



Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background



Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
Sample intent: background



Sample text:```This study supports the theory of a tumor origin from multipotential progenitor cells.(14) Reports of the Ki67 labeling index have demonstrated great variety.```
Sample intent: result



Sample text:```, 2009), are most abundant on endosomes undergoing the early-to-late transition (Rojas et al., 2008; Cullen and Korswagen, 2012; van Weering et al., 2012).```
Sample intent: method



Sample text:```This is interesting in relation to the result from our previous CGH study (comparing the number of significantly hybridizing probes on the microarray) that showed high sequence divergence on the Z chromosome between the common whitethroat and the zebra finch [62]; and such patterns are also found in other species [48,51,69].```
Sample intent: result