…transgenic lines harbouring 4 9 SARE after 24 h p.i. with Pto, and OFP fluorescence decreased in transgenic lines containing 4 9 PR1 after 24 h p.i. with Pto (Liu et al., 2013a); field evaluations yielded significantly induced OFP fluorescence only at or after 48 h postphytobacterial treatments.
Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
Sample intent: background
Sample text:```Our results show that after 4 h of thiotepa treatment, the level of expression of anti-apoptotic Bcl-2 was not changed; this confirms the results of a previous study [25].```
Sample intent: result



Sample text:```Similar results were obtained for several other plant species in previous studies (peanut, common bean, chestnut, black gram) (Raina et al. 2001; Marotti et al. 2007; Goulao et al. 2001, Souframanien and Gopalakrishna 2004).```
Sample intent: result



Sample text:```Instead of using the single fuzzless locus mutant developed mapping populations (Rong et al. 2005, 2007; Zhang et al. 2005; Guo et al. 2006; Wan et al. 2007), the common female parent line of the two F2 populations we developed combined at least two fuzzless loci (N1 and n2, and probably n3) (Turley and Kloth 2002).```
Sample intent: background



Sample text:```The effect of O2 appears to be mediated at least in part by prolyl 4-hydroxylation of Skp1, because elevated O2 levels are required by phyA – and Skp1-overexpression strains, and lower O2 is required by PhyA overexpression and Skp1B cells [10,24].```
Sample intent: method



Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
Sample intent: background
Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
Sample intent: background



Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background



Sample text:```Our results show that after 4 h of thiotepa treatment, the level of expression of anti-apoptotic Bcl-2 was not changed; this confirms the results of a previous study [25].```
Sample intent: result



Sample text:```MtrF has been proposed either to shuttle antimicrobials to the pump for export or to help stabilize one or more components of the efflux pump (56, 57).```
Sample intent: method



Sample text:```In the subsequent studies, Yoshida et al. (2004b) and Ishii et al. (2004) confirmed the reversibility of 2,6-dihydroxybenzoate decarboxylase of Rhizobium sp. strain MTP-10005 and R.radiobacter WU-0108, respectively.```
Sample intent: result



Sample text:```The effect of O2 appears to be mediated at least in part by prolyl 4-hydroxylation of Skp1, because elevated O2 levels are required by phyA – and Skp1-overexpression strains, and lower O2 is required by PhyA overexpression and Skp1B cells [10,24].```
Sample intent: method



Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background



Sample text:```The sgl gene has been shown to be expressed differentially during development (Binari et al. 1997; Haerry et al. 1997).```
Sample intent: method



Sample text:```MtrR also directly represses the mtrCDE efflux pump and rpoH (sigma 32) (57) (not shown in Fig.```
Sample intent: method



Sample text:```Similar results were obtained for several other plant species in previous studies (peanut, common bean, chestnut, black gram) (Raina et al. 2001; Marotti et al. 2007; Goulao et al. 2001, Souframanien and Gopalakrishna 2004).```
Sample intent: result