Repair of the breaks via the synthesisdependent strand annealing between sister chromatids could result in repeat instability (Richard et al., 2000, 2003).
Sample text:```Because most off-target nicks are not adjacent to each other and do not form DSBs, these nicks are repaired precisely and rarely lead to indels (Ran et al. 2013a; Shen et al. 2014).```
Sample intent: method
Sample text:```Because most off-target nicks are not adjacent to each other and do not form DSBs, these nicks are repaired precisely and rarely lead to indels (Ran et al. 2013a; Shen et al. 2014).```
Sample intent: method



Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
Sample intent: background



Sample text:```7C) that may correspond to the centrosomal katanin previously observed after nocodazole-mediated disruption of the spindle pole (McNally and Thomas, 1998).```
Sample intent: method



Sample text:```Therefore, one manifestation of the BRCA1–TONSL genetic interaction may well operate at sites of UV-induced damage, where both proteins are normally engaged in complex DNA damage responses (Duro et al. 2010; O’Connell et al. 2010; O’Donnell et al. 2010; Piwko et al. 2010; Pathania et al. 2011).```
Sample intent: method



Sample text:```This study supports the theory of a tumor origin from multipotential progenitor cells.(14) Reports of the Ki67 labeling index have demonstrated great variety.```
Sample intent: result
Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result



Sample text:```Methods for extraction, amplification and sequencing of the nuclear 5.8S rRNA gene and the two flanking, ITS regions were as previously published (Ganley et al., 2004; Shipunov et al., 2008).```
Sample intent: background



Sample text:```Our results confi rm previous studies that reached the same conclusion (Boushey et al 1994; Vermeulen et al 2000; Till et al 2005; Marcucci et al 2005).```
Sample intent: result



Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
Sample intent: background



Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background



Sample text:```Therefore, one manifestation of the BRCA1–TONSL genetic interaction may well operate at sites of UV-induced damage, where both proteins are normally engaged in complex DNA damage responses (Duro et al. 2010; O’Connell et al. 2010; O’Donnell et al. 2010; Piwko et al. 2010; Pathania et al. 2011).```
Sample intent: method



Sample text:```To further characterize isolates, random amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was performed using an adaptation of methods described elsewhere.(10)```
Sample intent: background



Sample text:```This study supports the theory of a tumor origin from multipotential progenitor cells.(14) Reports of the Ki67 labeling index have demonstrated great variety.```
Sample intent: result



Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background



Sample text:```7C) that may correspond to the centrosomal katanin previously observed after nocodazole-mediated disruption of the spindle pole (McNally and Thomas, 1998).```
Sample intent: method