The predicted secondary structure, obtained by using the program Mfold (Zuker, 2003), of this region indicated that it is composed of hairpin loop structures containing more than four hairpin loops (data not shown), whereas human parechovirus type 1 (Auvinen & Hyypiä, 1990) and HRV-2 (Pöyry et…
Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result
Sample text:```3, top) was constructed from the previously described 0.85-kb promoter construct (construct 6, Drews et al. 2005) by digestion with AscI and MluI followed by religation.```
Sample intent: method



Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background



Sample text:```This is interesting in relation to the result from our previous CGH study (comparing the number of significantly hybridizing probes on the microarray) that showed high sequence divergence on the Z chromosome between the common whitethroat and the zebra finch [62]; and such patterns are also found in other species [48,51,69].```
Sample intent: result



Sample text:```Mutation sites in the receptor-binding domain of the spike protein The spike protein of CoVs is responsible for cell receptor attachment and membrane fusion to mediate virus entry (Li, 2015).```
Sample intent: method



Sample text:```Discrepancies analogous to those between our results and those of simulations have, indeed, recently been observed in the case of the binary perceptron [18], the pspin interaction spin glass [19], and in the case of fluctuating manifolds in random media [20], where dynamically frozen phases were observed in regions of parameter space in which static approaches yielded ergodic phases.```
Sample intent: result
Sample text:```Discrepancies analogous to those between our results and those of simulations have, indeed, recently been observed in the case of the binary perceptron [18], the pspin interaction spin glass [19], and in the case of fluctuating manifolds in random media [20], where dynamically frozen phases were observed in regions of parameter space in which static approaches yielded ergodic phases.```
Sample intent: result



Sample text:```These results are in line with morphological abnormalities in a rabbit model of pneumococcal meningitis [8, 47], in neonatal and adult rat models [7, 51] and in human autopsy cases [20].```
Sample intent: result



Sample text:```This process is controlled not only by the protein-coding segments of the genome but also by non-coding regions, including loci that produce small RNAs (Grivna et al., 2006; He et al., 2009; Thomson and Lin, 2009).```
Sample intent: method



Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background



Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
Sample intent: background



Sample text:```Mutation sites in the receptor-binding domain of the spike protein The spike protein of CoVs is responsible for cell receptor attachment and membrane fusion to mediate virus entry (Li, 2015).```
Sample intent: method



Sample text:```Methods for extraction, amplification and sequencing of the nuclear 5.8S rRNA gene and the two flanking, ITS regions were as previously published (Ganley et al., 2004; Shipunov et al., 2008).```
Sample intent: background



Sample text:```The sequence of intermediates in the hR photocycle and their interconversions have been the subject of numerous studies (Va´ró et al., 1995a, b; Hazemoto et al., 1984; Zimányi and Lanyi, 1989a, b; Zima´nyi et al., 1989; Spencer and Dewey, 1990; Ames et al., 1992; Dioumaev and Braiman, 1997a).```
Sample intent: method



Sample text:```We screened for potential TF binding sites using STAMP [59], with the motifs’ position weight matrices (PWMs) retrieved from the TRANSFAC database [60].```
Sample intent: background



Sample text:```3, top) was constructed from the previously described 0.85-kb promoter construct (construct 6, Drews et al. 2005) by digestion with AscI and MluI followed by religation.```
Sample intent: method