Nevertheless, immunoglobulin light chains must exit cells in order to circulate freely: in this context, it is of great interest to consider the role of CD138 involved in exosome biogenesis, as these cargo containing vesicles seem to promote cancer development as well as other pathological conditions [49].
Sample text:```Intriguingly, using MPRs-deficient MEF cells, mouse and human CLN5 have been shown to reach lysosomes in an MPRindependent manner [12].```
Sample intent: background
Sample text:```Previous studies have shown that sialome genes were subject to strong ancient selective pressures that have resulted in species-specific adaptations (Varki 2001; Angata et al. 2004; Altheide et al. 2006; Varki & Gagneux 2009), suggesting that they likely evolved in response to ancient pathogens The function of sialome genes, i.```
Sample intent: result



Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
Sample intent: background



Sample text:```For experiments measuring the effect of Sch-28080 and ouabain on pHi recovery in ICs, only basolateral Cl removal was used to differentiate IC types (15, 17).```
Sample intent: background



Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result



Sample text:```…et al., 1981; Kopp et al., 1992; Tiruppathi et al., 1996; Blazejewski et al., 1997), a glycoprotein that has been shown to be involved in protein binding (especially of albumin) in other epithelia (Kopp et al., 1992; Blazejewski et al., 1997), could perform this function at the blood–CSF interface.```
Sample intent: method
Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
Sample intent: background



Sample text:```Previous studies have shown that sialome genes were subject to strong ancient selective pressures that have resulted in species-specific adaptations (Varki 2001; Angata et al. 2004; Altheide et al. 2006; Varki & Gagneux 2009), suggesting that they likely evolved in response to ancient pathogens The function of sialome genes, i.```
Sample intent: result



Sample text:```In our previous study, we found that RAG-mediated nicking and cleavage of oligonucleotide substrates containing cRSSs identified from lymphoid malignancies were either unchanged or slightly reduced in the presence of HMGB1 for most cRSSs tested (including LMO2, TAL1, Hox11, SIL, SCL), except Ttg-1 [24].```
Sample intent: method



Sample text:```Our genealogy, based on many alleles but only the first exon of bindin, is not entirely consistent with that of McCartney and Lessios (2004), based on fewer alleles but incorporating the entire molecule.```
Sample intent: result



Sample text:```Isolation of cardiomyocytes by standard enzymatic techniques and patch-clamp recordings were performed as described previously [42].```
Sample intent: background



Sample text:```The extracellular region of SIRPα interacts with its ligand, CD47, which is also a member of the Ig superfamily,(19,20,24) with such interaction promoting the tyrosine phosphorylation of SIRPα.(20,25) In contrast to the relatively restricted distribution of SIRPα, CD47 is expressed in most cell types including a variety of hematopoietic cells.```
Sample intent: method



Sample text:```, 2009), are most abundant on endosomes undergoing the early-to-late transition (Rojas et al., 2008; Cullen and Korswagen, 2012; van Weering et al., 2012).```
Sample intent: method



Sample text:```For experiments measuring the effect of Sch-28080 and ouabain on pHi recovery in ICs, only basolateral Cl removal was used to differentiate IC types (15, 17).```
Sample intent: background



Sample text:```Intriguingly, using MPRs-deficient MEF cells, mouse and human CLN5 have been shown to reach lysosomes in an MPRindependent manner [12].```
Sample intent: background



Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result