For GP60 analysis, PCR products were purified using the QIAquick PCR purification kit (Qiagen, USA) and sequenced (GATC Biotech AG, Germany).
Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result
Sample text:```This is interesting in relation to the result from our previous CGH study (comparing the number of significantly hybridizing probes on the microarray) that showed high sequence divergence on the Z chromosome between the common whitethroat and the zebra finch [62]; and such patterns are also found in other species [48,51,69].```
Sample intent: result



Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background



Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result



Sample text:```Similar results were obtained for several other plant species in previous studies (peanut, common bean, chestnut, black gram) (Raina et al. 2001; Marotti et al. 2007; Goulao et al. 2001, Souframanien and Gopalakrishna 2004).```
Sample intent: result



Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
Sample intent: background
Sample text:```This process is controlled not only by the protein-coding segments of the genome but also by non-coding regions, including loci that produce small RNAs (Grivna et al., 2006; He et al., 2009; Thomson and Lin, 2009).```
Sample intent: method



Sample text:```Our genealogy, based on many alleles but only the first exon of bindin, is not entirely consistent with that of McCartney and Lessios (2004), based on fewer alleles but incorporating the entire molecule.```
Sample intent: result



Sample text:```Similar results were obtained for several other plant species in previous studies (peanut, common bean, chestnut, black gram) (Raina et al. 2001; Marotti et al. 2007; Goulao et al. 2001, Souframanien and Gopalakrishna 2004).```
Sample intent: result



Sample text:```To further characterize isolates, random amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was performed using an adaptation of methods described elsewhere.(10)```
Sample intent: background



Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result



Sample text:```A linear relationship between erythrocyte GSH-Px and whole blood Se concentration was already described in camel [3, 8].```
Sample intent: method



Sample text:```In our previous study, we found that RAG-mediated nicking and cleavage of oligonucleotide substrates containing cRSSs identified from lymphoid malignancies were either unchanged or slightly reduced in the presence of HMGB1 for most cRSSs tested (including LMO2, TAL1, Hox11, SIL, SCL), except Ttg-1 [24].```
Sample intent: method



Sample text:```Our results confi rm previous studies that reached the same conclusion (Boushey et al 1994; Vermeulen et al 2000; Till et al 2005; Marcucci et al 2005).```
Sample intent: result



Sample text:```Methods for extraction, amplification and sequencing of the nuclear 5.8S rRNA gene and the two flanking, ITS regions were as previously published (Ganley et al., 2004; Shipunov et al., 2008).```
Sample intent: background



Sample text:```Results from a RCT assessing in-person post-test genomic counseling on 199 OSU-CMPC participants were published previously [18].```
Sample intent: method