All except the promoter region were determined to be significant by MACS.22 These 3 significant RUNX1-binding regions are in agreement with recently published RUNX1 ChIP-seq data using murine HSPC lines.31 The binding of RUNX1 to these regions was confirmed using ChIP-qPCR (Figure 2H).
Sample text:```In our previous study, we found that RAG-mediated nicking and cleavage of oligonucleotide substrates containing cRSSs identified from lymphoid malignancies were either unchanged or slightly reduced in the presence of HMGB1 for most cRSSs tested (including LMO2, TAL1, Hox11, SIL, SCL), except Ttg-1 [24].```
Sample intent: method
Sample text:```Our genealogy, based on many alleles but only the first exon of bindin, is not entirely consistent with that of McCartney and Lessios (2004), based on fewer alleles but incorporating the entire molecule.```
Sample intent: result



Sample text:```This study supports the theory of a tumor origin from multipotential progenitor cells.(14) Reports of the Ki67 labeling index have demonstrated great variety.```
Sample intent: result



Sample text:```In our previous study, we found that RAG-mediated nicking and cleavage of oligonucleotide substrates containing cRSSs identified from lymphoid malignancies were either unchanged or slightly reduced in the presence of HMGB1 for most cRSSs tested (including LMO2, TAL1, Hox11, SIL, SCL), except Ttg-1 [24].```
Sample intent: method



Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
Sample intent: background



Sample text:```This means that accumulation of the CMG helicase with hyperphosphorylated Psf1 is a sensitive marker for the aberrant firing of late-origins in cells treated with hydroxyurea (42), reflecting a specific failure to activate the S-phase checkpoint response at some point downstream of the Mec1 kinase.```
Sample intent: method
Sample text:```Our results confi rm previous studies that reached the same conclusion (Boushey et al 1994; Vermeulen et al 2000; Till et al 2005; Marcucci et al 2005).```
Sample intent: result



Sample text:```3, top) was constructed from the previously described 0.85-kb promoter construct (construct 6, Drews et al. 2005) by digestion with AscI and MluI followed by religation.```
Sample intent: method



Sample text:```This means that accumulation of the CMG helicase with hyperphosphorylated Psf1 is a sensitive marker for the aberrant firing of late-origins in cells treated with hydroxyurea (42), reflecting a specific failure to activate the S-phase checkpoint response at some point downstream of the Mec1 kinase.```
Sample intent: method



Sample text:```This process is controlled not only by the protein-coding segments of the genome but also by non-coding regions, including loci that produce small RNAs (Grivna et al., 2006; He et al., 2009; Thomson and Lin, 2009).```
Sample intent: method



Sample text:```The PCR product was digested with AgeI/NotI and ligated between the G and the L gene (similar to Roberts et al., 1999) into the vectors pVSVCT-1 and pVSVCT-9 (Schnell et al., 1998), which had been digested with XmaI/NotI.```
Sample intent: background



Sample text:```In our previous study, we found that RAG-mediated nicking and cleavage of oligonucleotide substrates containing cRSSs identified from lymphoid malignancies were either unchanged or slightly reduced in the presence of HMGB1 for most cRSSs tested (including LMO2, TAL1, Hox11, SIL, SCL), except Ttg-1 [24].```
Sample intent: method



Sample text:```Instead of using the single fuzzless locus mutant developed mapping populations (Rong et al. 2005, 2007; Zhang et al. 2005; Guo et al. 2006; Wan et al. 2007), the common female parent line of the two F2 populations we developed combined at least two fuzzless loci (N1 and n2, and probably n3) (Turley and Kloth 2002).```
Sample intent: background



Sample text:```Bork et al. (2010) reported that highly significant differences in methylation patterns were observed at specific CpG sites, such as those near homeobox genes between early and late cultures.```
Sample intent: method



Sample text:```We screened for potential TF binding sites using STAMP [59], with the motifs’ position weight matrices (PWMs) retrieved from the TRANSFAC database [60].```
Sample intent: background



Sample text:```Unlabeled DNA substrates containing consensus 12-RSS, 23-RSS, Hox11 or non-specific DNA sequences (DAR81/82) were prepared from oligonucleotides described previously [11,24].```
Sample intent: background