Real-time RT-PCR techniques were used to analyze mRNA expression using the Strategen MX3000, as previously reported (3, 4, 7).
Sample text:```Our results further confirm previous findings on the differential regulatory mechanisms for Hoxa3 and Hoxb3 expression in r5 and r6 by kreisler (Manzanares et al., 1997, 1999a,b, 2001).```
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Sample text:```To further characterize isolates, random amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was performed using an adaptation of methods described elsewhere.(10)```
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Sample text:```3, top) was constructed from the previously described 0.85-kb promoter construct (construct 6, Drews et al. 2005) by digestion with AscI and MluI followed by religation.```
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Sample text:```Results from a RCT assessing in-person post-test genomic counseling on 199 OSU-CMPC participants were published previously [18].```
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Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
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Sample text:```Our results confi rm previous studies that reached the same conclusion (Boushey et al 1994; Vermeulen et al 2000; Till et al 2005; Marcucci et al 2005).```
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Sample text:```Amplicon preparation, sequencing and data analysis were conducted for all 44 samples as described previously (Hospodsky et al., 2012).```
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Sample text:```This process is controlled not only by the protein-coding segments of the genome but also by non-coding regions, including loci that produce small RNAs (Grivna et al., 2006; He et al., 2009; Thomson and Lin, 2009).```
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Sample text:```3, top) was constructed from the previously described 0.85-kb promoter construct (construct 6, Drews et al. 2005) by digestion with AscI and MluI followed by religation.```
Sample intent: method



Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
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Sample text:```Our results confi rm previous studies that reached the same conclusion (Boushey et al 1994; Vermeulen et al 2000; Till et al 2005; Marcucci et al 2005).```
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Sample text:```Results from a RCT assessing in-person post-test genomic counseling on 199 OSU-CMPC participants were published previously [18].```
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Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
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Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
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Sample text:```Our results show that after 4 h of thiotepa treatment, the level of expression of anti-apoptotic Bcl-2 was not changed; this confirms the results of a previous study [25].```
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Sample text:```These results are in line with morphological abnormalities in a rabbit model of pneumococcal meningitis [8, 47], in neonatal and adult rat models [7, 51] and in human autopsy cases [20].```
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