B16 cells were treated with TGFb1 (0, 20, 100, 200, 400 or 1000 pM) or activin A (0, 0.04, 0.2, 1, 2,
4 nM) for 24 h. Gene expression of E-cadherin was quantified by quantitative real-time RT-PCR (b).
Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
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Sample text:```Illustra PuReTaq ReadyTo-Go PCR beads (0.5 ml tubes, GE healthcare) were used to amplify DTL, SLFN5, TRIM47, WRAP53 and CHN1 transcripts (denaturing at 94uC for 30 s, annealing at 58uC for 1 min and elongation at 72uC for 2 min, 25‚Äì35 cycles).```
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Sample text:```Isolation of cardiomyocytes by standard enzymatic techniques and patch-clamp recordings were performed as described previously [42].```
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Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
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Sample text:```Basal ATP concentrations in the bulk medium of unstimulated CFs are low, but the concentrations of released nucleotides near the cell surface may be sufficient to activate adjacent P2Y receptors and contribute to basal signaling (48, 49).```
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Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
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Sample text:```This study supports the theory of a tumor origin from multipotential progenitor cells.(14) Reports of the Ki67 labeling index have demonstrated great variety.```
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Sample text:```Our results further confirm previous findings on the differential regulatory mechanisms for Hoxa3 and Hoxb3 expression in r5 and r6 by kreisler (Manzanares et al., 1997, 1999a,b, 2001).```
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Sample text:```We screened for potential TF binding sites using STAMP [59], with the motifs’ position weight matrices (PWMs) retrieved from the TRANSFAC database [60].```
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Sample text:```ly), which have been originally identified as molecules activated by growth factor-stimulation of fibroblusts, are key kinases in the intracellular signal transduction pathways (1-6).```
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Sample text:```b-catenin normally associates with E-cadherin and with a-catenin and thereby participates in the regulation of cell-to-cell adhesion (Vleminckx and Kemler 1999; Miyoshi and Takai 2005).```
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Sample text:```Two days after transfection, cells were harvested and D2 activity was measured in lysates using HPLC, as described previously (20).```
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Sample text:```Our results show that after 4 h of thiotepa treatment, the level of expression of anti-apoptotic Bcl-2 was not changed; this confirms the results of a previous study [25].```
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Sample text:```…proteins initially has been demonstrated in tissue culture model systems of adipogenesis and hematopoiesis (Lin and Lane 1992; Freytag et al. 1994; Hu et al. 1995; Müller et al. 1995; Nerlov et al. 1998) and has now been firmly established through analysis of the respective knockout mice…```
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Sample text:```Isolation of cardiomyocytes by standard enzymatic techniques and patch-clamp recordings were performed as described previously [42].```
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Sample text:```Because previous studies indicated that hexapeptides bind the junction core (29, 32), we tested two junctions, one with an AT-rich core and the other with a GC-rich core (Fig.```
Sample intent: result