It was reported that ESCs may be short or long term cultivated on synthetic substrates in com mercial chemically defined media (Brafman et al., 2010; Mahlstedt et al., 2010; Higuchi et al., 2011; Lee et al., 2011; Nandivada et al., 2011; Valamehr et al., 2011; Ueda et al., 2012).