The list of significantly enriched TFs included developmental factors such as Hairy, which plays a critical role in the segmentation of the early embryo (Nusslein-Volhard and Wieschaus 1980), Fruitless, which promotes male-specific neural development and behavior (Manoli et al. 2005), as well as Homothorax, a Hox protein co-factor (Ryoo et al.