Furthermore, GFP-ATG16L1△(1–320) precipitated mCherryTMEM74 and endogenous TMEM74 (Figure 2e), conversely, GFP-ATG16(1-320) failed to bindmCherry-TMEM74 (Figure 2f), suggesting that the WD repeats domain of ATG16L1 was required for the interaction with TMEM74.