They comprised 136 strains isolated from clinical environments; 42 strains isolated from intertidal sand contaminated by Prestige‚Äôs crude oil [23]; 50 strains from our laboratory collection, which have been previously studied by multilocus sequence analysis, MLSA [6, 20, 22]; and strain PE, which was isolated from a bioreactor seeded with the commercial compound Putidoil¬Æ.