text:
Commencement and citation
1
This Order may be cited as the A3 Trunk Road (Merton) Red Route Traffic Order 1996, and shall come into force on 16th September 1996.
Interpretation
2
In this Order —
a
a reference to an article or a schedule followed by a number is a reference to the article of, or the schedule to, this Order so numbered;
b
“carriageway” has the same meaning as in section 329(1) of the Highways Act 1980 ;
c
causing includes permitting;
d
“disabled person”, “disabled person’s badge” and “disabled person’s vehicle” have the same meanings as in regulation 2(1) of the Local Authorities' Traffic Orders (Exemptions for Disabled Persons) (England and Wales) Regulations 1986 , and “relevant position” in relation to a disabled person’s badge has the same meaning as in regulation 2A of those regulations;
e
“the trunk road red route” means the lengths of road specified in column 2 of the table in schedule 1;
f
“vehicle” includes part of a vehicle; and
g
an entry in column (3), (4) or (5) of an item in a schedule to this order applies to that column in subsequent items in that schedule.
General prohibition of stopping
3
1
Subject to the provisions of paragraphs (2) and (3) of this article, and of articles 4, 5, 6, 7, 8 and 9, no person shall cause any vehicle to stop in the trunk road red route during the restricted hours.
2
Subject to the provisions of paragraph (3) of this article, and of articles 5(1), 8 and 9, no person shall cause any vehicle to stop at any time in any lengths of the trunk road red route specified in schedule 4.
3
The controls specified in paragraphs (1) and (2) of this article do not apply in respect of any area of the trunk road red route specified in schedule 2A.
4
In this article “the restricted hours” means, in relation to a length of the trunk road red route specified in an item in column (2) of the table in schedule 1, the hours specified in that item in column (3) of that table.
Exemptions for parking
4
The controls specified in article 3(1) do not apply in respect of a person causing a vehicle to be parked at the edge of the carriageway on a length of road specified in an item in column (2) of the table in schedule 2B during the hours specified in column (3) in that item for a period not exceeding the period specified in column (4) in that item, provided that not less than the period specified in column (5) in that item has elapsed since the vehicle was last parked on any part of the same length of road.
Miscellaneous exemptions for parking
5
1
Licensed taxi cabs: The controls specified in article 3(1) and (2) do not apply in respect of a person causing a vehicle being used under a licence under section 6 of the Metropolitan Public Carriage Act 1869 to be parked at the edge of the carriageway on a length of road specified in schedule 2D.
2
Coaches: The controls specified in article 3(1) do not apply in respect of a person causing a motor vehicle constructed or adapted to carry more than 8 passengers (exclusive of the driver) or a local bus (as defined in article 8(4)) not so constructed or adapted, to be parked at the edge of the carriageway on a length of road specified in an item in column (2) of the table in schedule 2E during the hours specified in column (3) in that item for a period not exceeding the period specified in column (4) in that item, provided that not less than the period specified in column (5) in that item has elapsed since the vehicle was last parked on any part of the same length of road.
3
Doctors' vehicles: The controls specified in article 3(1) do not apply in respect of a person causing a vehicle to be parked at the edge of the carriageway on a length of road specified in an item in column (2) of the table in schedule 2F during the hours specified in column (3) in that item, provided that —
a
there is displayed in the windscreen of the vehicle a badge issued by, or with the authority of, the Traffic Director for London to a medical practitioner, and which specifies —
i
the name of the person to whom it was issued, and
ii
the premises in relation to which it applies; and
b
the vehicle was parked by, or with the permission of, the person whose name is specified on the badge and for so long only as the person who parked the vehicle is engaged in work at the premises specified on the badge.
4
Solo motor bicycles. The controls specified in article 3(1) do not apply in respect of a person causing a motor bicycle without a side-car to be parked at the edge of the carriageway on a length of road specified in an item in column (2) of the table in schedule 2G during the hours specified in column (3) in that item.
Exemptions for loading and unloading
6
The controls specified in article 3(1) do not apply in respect of a person causing a vehicle which is being loaded or unloaded to stop at the edge of the carriageway on a length of road specified in an item in column (2) of the tables in schedules 3A and 3B during the hours specified in column (3) in that item for as long as is needed to load or unload the vehicle or 20 minutes, whichever is the less, provided that not less than 20 minutes has elapsed since the vehicle was last parked on any part of the same length of road.
Exemptions for disabled persons
7
1
The controls specified in article 3(1) do not apply in respect of a person causing a vehicle which displays a disabled person’s badge in the relevant position to park at the edge of the carriageway on a length of road specified in an item in column (2) of the table in schedule 2B during the hours specified in column (3) in that item.
2
The controls specified in article 3(1) do not apply in respect of a person causing a vehicle which displays a disabled person’s badge in the relevant position to park at the edge of the carriageway on a length of road specified in an item in column (2) of the table in schedule 2C during the hours specified in column (3) in that item.
3
The controls specified in article 3(1) do not apply in respect of a person causing a vehicle which displays a disabled person’s badge in the relevant position, and a parking disc on which is shown the time at which the period of parking began, to park at the edge of the carriageway on a length of road specified in an item in column (2) of the table in schedule 3A during the hours specified in column (3) in that item, for a period of up to 3 hours not being a period separated by an interval of less than 1 hour from a previous period when that vehicle was parked in that length of road.
Exemptions at bus stops and stands
8
1
The controls specified in article 3(1) and (2) do not apply in respect of a person causing a vehicle to which this article applies to stop at a bus stop (being an area of road bounded by a traffic sign which includes the words “bus stop”) for so long only as may be required to enable passengers to get on or off the vehicle or, in the case of a vehicle being used to provide a local service, for so long only as may be required for a change of crew.
2
The controls specified in article 3(1) and (2) do not apply in respect of a person causing a vehicle being used to provide a local service to stop at a bus stand (being an area of road bounded by a traffic sign which includes the words “bus stand”) for so long only as may be required for timetabling reasons.
3
This article applies —
a
before 1 January 1997 to
i
a public service vehicle used for the provision of a local service or a scheduled express service,
ii
a school bus, and
iii
a works bus; and
b
after 31 December 1996 to
i
a motor vehicle constructed or adapted to carry more than 8 passengers (exclusive of the driver), and
ii
a local bus not so constructed or adapted.
4
In this article “local bus”, “local service”, “scheduled express service”, “school bus” and “works bus” have the meanings given to those expressions in regulation 4 of the Traffic Signs Regulations and General Directions 1994 .
Other exemptions
9
1
The controls specified in article 3(1) and (2) do not apply in respect of a person causing a vehicle to stop if that person is —
a
required by law to cause the vehicle to stop or not to proceed;
b
obliged to stop the vehicle so as to avoid an accident;
c
prevented from proceeding in the vehicle by circumstances beyond the person’s control;
d
getting or giving help in consequence of an accident or an emergency, or otherwise taking action for public safety;
e
opening or closing a gate or other barrier at the entrance to premises to which the vehicle is being driven or from which it has emerged if it is not reasonably practicable to cause the vehicle to stop elsewhere for the purpose;
f
using the vehicle for fire brigade, ambulance or police purposes;
g
using the vehicle in connection with the removal of any obstruction to traffic;
h
using the vehicle in connection with the maintenance, improvement or reconstruction of any length of road specified in schedule 1;
i
using the vehicle under a licence under section 6 of the Metropolitan Public Carriage Act 1869 to stop for so long only as may be required to enable a passenger to get on or off the vehicle; or
j
using a disabled person’s vehicle which displays a disabled person’s badge in the relevant position to stop for so long as may be required to enable a disabled person to get on or off the vehicle.
2
The controls specified in article 3(1) and (2) do not apply in respect of a vehicle which bears the Royal Mail livery which is waiting at the edge of a carriageway for as long as may be necessary for the purpose of collecting or delivering letters in pursuance of any statutory duty of the Post Office to collect or deliver letters.
3
The controls specified in article 3(1) and (2) do not apply to anything done with the permission or at the direction of a police constable in uniform or a traffic warden.
Revocation of existing Orders
10
1
The Greater London Council 1977 No 107 The Merton (Waiting and Loading) Order 1977 and the A3 Trunk Road (Merton) Red Route Experimental Traffic Order 1995 are hereby revoked, in so far as their provisions relate to any part of the trunk road red route.
Signed in accordance with a direction from the Secretary of State for Transport
Derek Turner
Traffic Director for London
9th September 1996


type: legislation

jurisdiction: UK

text:
Title and commencement
1
These Regulations may be cited as the Medicines (Animal Feeding Stuffs) (Enforcement) (Amendment) Regulations 1996 and shall come into force on 1st June 1996.
Amendment of the Medicines (Animal Feeding Stuffs) (Enforcement) Regulations 1985
2
1
The Medicines (Animal Feeding Stuffs) (Enforcement) Regulations 1985 shall be amended in accordance with the following paragraphs of this regulation.
2
In regulation 4 (Methods of analysis) —
a
in paragraph (1), after “Paragraphs (2)” there shall be inserted “(2A)”;
b
for paragraph (2) the following paragraphs shall be substituted —
2
For the purpose of determining what quantity or proportion (if any) of a substance of a description or class listed in column 1 of Table A in division 3, or in divisions 4 and 5, of Schedule 3 to these Regulations has been incorporated in a sample of an animal feeding stuff to be analysed pursuant to sections 112, 113 or 115 of the Act, the provisions of divisions 1 and 2 of the said Schedule 3 shall apply, and —
a
in relation to a substance of a description or class listed in column 1 of Table A in division 3, the method of analysis set out in the Community provision specified in the corresponding entry of column 2 of that Table shall be used; and —
b
in relation to substances respectively specified in divisions 4 and 5 of the said Schedule, the methods of analysis respectively set out in those divisions shall be used.
2A
Where a reference to a Directive is made in column 2 of Table A of division 3 of Schedule 3 to these Regulations for which there is an entry in column 1 of Table B of that division, the reference shall be construed as being a reference to the Commission Directive establishing or relating to Community methods of analysis for the official control of feeding stuffs described in the corresponding entries in columns 2 and 3 of Table B.; and
c
in paragraph (3), after “paragraph (2)”, there shall be inserted “or (2A)”.
3
In Schedule 3 (Methods of analysis) —
a
for items 3 to 28 inclusive of the list of main divisions the following items shall be substituted —
3
Community methods of analysis
4
Determination of meticlorpindol
5
Determination of nifursol
; and
b
for divisions 3 to 28 inclusive the following divisions shall be substituted —
3.
COMMUNITY METHODS OF ANALYSIS
Table A–
Methods of Analysis
Column (l) Column (2) Substance Community provision
Antibiotics of the tetracycline group Part 1 of Annex II to Directive 72/199/ EEC Amprolium Part 1 of Annex II to Directive 74/203/EEC Avoparcin Part 1 of the Annex to Directive 81/715/EEC Buquinolate Part 1 of the Annex to Directive 75/84/EEC Chlortetracycline oxytetracycline and tetracycline Part 2 of Annex II to Directive 72/199/EEC Copper Part 3 of the Annex to Directive 78/633/EEC Dinitolmide (DOT) Part 3 of Annex II to Directive 74/203/EEC Ethopabate Part 2 of Annex II to Directive 74/203/EEC Flavophospholipol Part 2 of the Annex to Directive 78/633/EEC Furazolidone Part 3 of the Annex to Directive 75/84/EEC Halofuginone Annex to Directive 93/70/EEC Methyl benzoquate Part 2 of the Annex to Directive 93/117/ EC Monensin sodium Part 2 of the Annex to Directive 81/715/EEC Nicarbazin Part 4 of Annex II to Directive 74/203/EEC Oleandomycin Part 3 of Annex II to Directive 72/199/EEC Robenidine Part 1 of the Annex to Directive 93/117/EC Spiramycin Annex to Directive 84/425/EEC Sulphaquinoxaline Part 2 of the Annex to Directive 75/84/EEC Tylosin Part 4 of Annex II to Directive 72/199/EEC Virginiamycin Part 5 of Annex II of Directive 72/199/EEC as replaced by Article 2 of,, and Annex II to,, Directive 84/4/EEC Zinc bacitracin Part 1 of the Annex to Directive 78/633/EEC as replaced by Article 3 of,, and Annex III to,, Directive 84/4/EEC
Table B–
Interpretation
Column (1) Column (2) Column (3) Directive reference Commission Directive Reference
Directive 72/199/EEC Third Commission Directive 72/199/EEC OJ No. L 123,, 29.5.72,, p.6 (OJ/SE 1966-72 p.74) Directive 74/203/EEC Fifth Commission Directive 74/203/EEC OJ No. L 108,, 22.4.74,, p.7 Directive 75/84/EEC Sixth Commission Directive 75/84/EEC OJ No. L 32,, 5.2.75,, p.26 Directive 78/633/EEC Eight Commission Directive 78/633/EEC OJ No. L 206,, 29.7.78,, p.43 Directive 81/715/EEC Ninth Commission Directive 81/715/EEC OJ No. L 257,, 10.9.81,, p.38 Directive 84/4/EEC Commission Directive 84/4/EEC OJ No. L 15,, 18.1.84,, p.28 Directive 84/425/EEC Tenth Commission Directive 84/425/EEC OJ No. L 238,, 6.9.84,, p.34 Directive 93/70/EEC Eleventh Commission Directive 93/70/EEC OJ No. L 234,, 17.9.93,, p.l7 Directive 93/117/EC Twelfth Commission Directive 93/117/EC OJ No. L 329,, 30.12.93,, p.54
4.
DETERMINATION OF METICLORPINDOL
(3,5-dichloro-2,6-dimethylpyridine-4-ol)
SCOPE AND FIELD OF APPLICATION
1
The method is for the determination of the quantity of meticlorpindol in complete feeding stuffs, protein concentrates and feed supplements. The lower limit of the determination is 60mg/kg.
PRINCIPLE
2
Meticlorpindol is extracted from the feed with methanolic ammonia solution, and a portion of the extract is passed through a column of aluminium oxide onto a column of ion-exchange resin. The meticlorpindol is retained on the resin and interfering substances are removed by washing with 80% methanol. The meticlorpindol is eluted from the resin with 40% acetic acid and the absorbance is measured at 267nm.
REAGENTS
3
Note: The suitability of a batch of aluminium oxide and of the other reagents should be tested before use by analysing a blank feed to which a known amount of meticlorpindol has been added.
3.1
Aluminium oxide for column chromatography, 100 to 250 mesh, alkaline, Brockman activity 1.
3.2
Ammonia (density 0.88g per ml).
3.3
Anion exchange resin, AG1-X8 or Dowex 1-X8, 100 to 200 mesh — To convert Dowex resin in the chloride form to the acetate form add 1 litre of 6M hydrochloric acid to 350g of resin in a 3 litre beaker, and heat the mixture on a steam bath for 2 to 3 hours. Pour the slurry into a glass Buchner funnel, and wash the resin with water until the washings are free from chloride (about 6 litres of water are required). Transfer the resin to a 50mm diameter glass column having a coarse sintered-glass disc at the bottom end, and wash with sodium acetate solution (5g sodium acetate, anhydrous, dissolved in water and diluted to 100ml) until the column effluent gives only a cloudy solution on addition of silver nitrate solution. Return the resin to the glass Buchner funnel, and wash with water. Transfer the resin to a 3 litre beaker, add 1 litre of 40% v/v acetic acid solution (3.4) and heat on a steam bath for 3 hours or longer. Filter, and wash the resin again with water until the washings are free from chloride. Store the resin in water.
3.4
Acetic acid solution 40% v/v.
3.5
Methanol.
3.6
Methanol solution 80% v/v.
3.7
Ammoniacal methanol solution: dilute 1 volume of ammonia (3.2) with 19 volumes of methanol (3.5).
3.8
Meticlorpindol standard solution: weigh, to the nearest 0.lmg, 125mg of meticlorpindol into a beaker, add 25ml of sodium hydroxide solution (2g sodium hydroxide dissolved in water and diluted to 100ml) to dissolve the meticlorpindol, transfer the solution to a 500ml graduated flask, and dilute to the mark with water. This solution contains 250mg per ml meticlorpindol.
APPARATUS
4
4.1
Aluminium oxide column: constructed as indicated in the diagram overleaf.
4.2
Ion exchange column: constructed as indicated in the diagram overleaf.
4.3
Spectrophotometer, recording, with 10mm silica cells.
PROCEDURE
5
Extraction of meticlorpindol
5.1
Weigh, to the nearest 0.001g, approximately 50g of the finely divided and mixed sample, or a suitable amount expected to contain about 12mg of meticlorpindol, transfer to a 500ml graduated flask, and add 400ml of ammoniacal methanol solution (3.7). Place a magnetic stirring bar in the flask and stir the mixture on a magnetic stirrer for 20 minutes. Remove the stirring bar from the flask, dilute to the mark with ammoniacal methanol solution (3.7), mix the contents well, and set aside for 20 to 30 minutes.
Purification
5.2
5.2.1
Aluminium oxide column: For each column required weigh approximately 25g of aluminium oxide (3.1) into an aluminium foil dish and place in an oven at 105°C for 1 hour. Remove the dish from the oven and cool to room temperature in a desiccator. Make a slurry of the aluminium oxide with 25ml of ammoniacal methanol solution (3.7) and filter on a Buchner funnel. Wash the aluminium oxide with methanol (3.5) until the washings are neutral. Form a slurry of the aluminium oxide with 50ml of methanol (3.5) and pour the slurry into the column (4.1). Allow the methanol to drip through the column. Place a plug of glass wool lightly on top of the aluminium oxide and then wash with 25ml of methanol (3.5). Do not allow the liquid in the column to fall below the top of the aluminium oxide. Discard the eluate.
5.2.2
Anion exchange column: Form a slurry in acetic acid (3.4) of sufficient resin (3.3) to fill the columns required. Filter on a Buchner funnel, wash the resin with twice its own volume of acetic acid (3.4) and then with aqueous methanol (3.6) until the washings are neutral. Form a slurry of the resin with aqueous methanol (3.6) and add sufficient to a column (4.2) to give a resin bed 20 to 30 mm deep after settling. Place a small plug of glass wool on top of the resin and wash the column with two 13ml portions of aqueous methanol (3.6). Do not allow the liquid level in the column to fall below the top of the resin. Discard the eluate.
5.2.3
Chromatographic procedure: By pipette transfer 10.0ml of the extract of the feed sample (5.1) directly onto an aluminium oxide column and at the same time transfer the same volume of ammoniacal methanol solution (3.7) directly onto a second aluminium oxide column (reagent blank). Allow the solutions to drain to the top of the aluminium oxide and then wash each column with three 12ml portions of aqueous methanol (3.6), allowing the liquid to drain to the top of the aluminium oxide each time. Let all the eluate from each column drain directly into separate ion-exchange columns, and then remove the aluminium oxide columns. Allow the liquid to drain to the top of the ion-exchange resin and then wash each column with four 13ml portions of aqueous methanol (3.6). Discard the eluates.
Elute each column with two 10ml and then one 4ml portions of acetic acid (3.4). Collect the eluates from each column in separate 25ml graduated flasks and dilute the contents of each to the mark with acetic acid (3.4).
Determination
5.3
Record the absorption spectrum of the sample extract between 350 and 245nm in 10mm silica cells with the reagent blank solution (5.2.3) as reference. Measure the absorbance of the sample extract at 267nm above a baseline obtained by drawing a line through the absorbance at 327 and 297nm and extending it through 267nm.
(Note: Background absorption due to the feed approaches a linear function that can be described by the points on the curve at 296 and 327nm. Occasionally this is not the case, as can be detected by absorption peaks in the region between 350 and 297nm).
Determine the concentration of meticlorpindol in the sample by reference to the calibration curve (5.4).
Calibration curve
5.4
By pipette transfer 1, 5, 7.5, 10, 12.5 and 15ml portions of meticlorpindol standard solution (3.8) to separate 250ml graduated flasks. Dilute the contents of each flask to the mark with acetic acid (3.4). Record the absorption spectra of these solutions in 10 mm silica cells between 350 and 245nm with acetic acid (3.4) as reference. Construct a calibration curve using the absorbances at 267nm as ordinates and the corresponding concentrations of meticlorpindol in mg per ml as abscissae.
CALCULATION OF RESULTS
6
The meticlorpindol content in mg/ kg of sample is given by the formula:
23.23 × C × 50 W
in which:
C
concentration of meticlorpindol, in mg per ml, read from the calibration curve equivalent to the absorbance of the test solution;
23.23
a factor that makes allowance for the volume of the feed sample in the flask; and
W
weight of test portion in g.
Absorbance at 327 and 297nm should not differ by more than 0.05 units and both points should be below 0.2. Results should be satisfactory as long as these criteria are kept in mind along with any obvious distortion in the appearance of the curve. No maximum other than that of meticlorpindol should be present.
5.
DETERMINATION OF NIFURSOL
[3,5-dinitro-2-(5-nitrofurfurylidene)salicylohydrazide]
SCOPE AND FIELD OF APPLICATION
1
The method is for the determination of the quantity of nifursol in complete feeding stuffs, protein concentrates and feed supplements. Other substances that will provide a nitro group under the conditions of the method, eg nitrofurazone and furazolidone, will interfere. The lower limit of the determination is 20mg/kg.
PRINCIPLE
2
The sample is extracted with dimethylformamide and the extract is purified on a column of aluminium oxide. A portion of the purified extract containing the nifursol is treated with phenylhydrazine hydrochloride and the resulting phenylhydrazone extracted into toluene. The addition of methylbenzethonium hydroxide to the toluene solution produces a blue colour, the absorbance of which is measured as 515nm.
REAGENTS
3
3.1
Toluene.
3.2
Aluminium oxide for column chromatography, 80 to 200 mesh, alkaline, Brockman activity 1. To 100 parts of the aluminium oxide add 6 parts of powdered magnesium hydroxide. Shake in a screw-cap bottle to mix, add 8 parts of water, and mix until free from lumps.
3.3
Sand; acid washed.
3.4
Dimethylformamide solution, 95% v/v.
3.5
Dimethylformamide solution, 50% v/v.
3.6
Phenylhydrazine, hydrochloride solution: shake 0.25 0.005g of phenylhydrazine hydrochloride in 25ml of water, add 25ml of concentrated hydrochloric acid, and shake to dissolve the solid, filtering if necessary. Prepare this reagent immediately before use.
3.7
Methylbenzethonium hydroxide solution: about 10% in methanol.
3.8
Nifursol standard solution: weigh, to the nearest 0.1 mg, 25mg of pure nifursol into a 100ml graduated flask, add 5ml of 95% v/v dimethylformamide solution (3.4), and mix until all the solid has dissolved. Dilute to the mark with methanol. Prepare this solution freshly each day.
APPARATUS
4
4.1
Chromatographic column — A glass column, internal diameter: 20 to 25mm; length: 100 to 150mm plugged at the lower end with glass wool.
4.2
Spectrophotometer, with 10mm cells.
PROCEDURE
5
Extraction
5.1
Weigh to the nearest 0.001g, approximately 5g of the finely divided and mixed sample, or a suitable amount expected to contain about 350 mg of nifursol and transfer to a 125ml conical flask. Add 50.0ml of 95% v/v dimethylformamide solution (3.4), insert a stopper loosely, and place the flask in a water-bath at 60°C for 30 minutes. Swirl the contents of the flask occasionally during this period. Shake the flask on a mechanical shaker for 30 minutes and then filter the contents through a rapid filter-paper, preferably under reduced pressure on a Buchner funnel. Transfer 40.0ml of the filtrate to a beaker, add 40.0ml of water, and stir. Set the beaker aside, protected from light, for 30 minutes.
Purification
5.2
Pack the chromatographic column (4.1) to a depth of 70mm with the prepared aluminium oxide (3.2) and on top of the aluminium oxide add a layer of sand (3.3) 15mm deep. Wash the column with 50ml of 50% v/v dimethylformamide solution (3.5) and then pass the dimethylformamide extract of the test sample through the column; reject the first 45ml of eluate and collect the next 17ml.
Determination
5.3
Pipette 5.0ml of the eluate to a 20ml centrifuge tube, add 5ml of phenylhydrazine hydrochloride solution (3.6), mix, and place the tube in a water-bath at 40°C for 20 minutes. Remove the tube from the water-bath and cool it in running water for 5 minutes. Add 5.0ml of toluene (3.1) to the contents of the tube, insert a glass or plastic stopper (a rubber stopper must not be used), and shake vigorously 40 times. Centrifuge for 5 minutes to clear the toluene layer, and transfer 3.0ml of the toluene layer to a 10mm spectrophotometer cell. Add 0.2ml of methylbenzethonium hydroxide solution (3.7), mix immediately, and measure the absorbance of the solution within one minute at 515nm with toluene as reference. Determine the quantity of nifursol by reference to the calibration curve (5.4).
Calibration curve
5.4
Pipette 5.0ml of nifursol standard solution (3.8) to a 200ml graduated flask, add 100ml of 95% v/v dimethylformamide solution (3.4), dilute to the mark with water and mix. Into separate 20ml centrifuge tubes transfer by pipette 1, 2, 3, 4 and 5 ml portions of this solution and dilute the contents of each tube to 5ml with 50% v/v dimethylformamide solution (3.5).
Treat the contents of each tube as described under “Determination” (5.3) beginning at “…. add 5ml of phenylhydrazine hydrochloride solution (3.6)…”. Plot the calibration curve using the absorbances as the ordinates and the corresponding quantities of nifursol in mg as abscissae.
CALCULATION OF RESULTS
6
The nifursol content in mg/kg of sample is given by the formula:
20 × A W
in which:
A
μg of nifursol read from the calibration curve; and
W
weight of the test portion in g.
Angela Browning
Parliamentary Secretary, Ministry of Agriculture, Fisheries and Food
1st May 1996
Lindsay
Parliamentary Under Secretary of State, Scottish Office
30th April 1996
Signed by authority of the Secretary of State for Wales
Gwilym Jones
Parliamentary Under Secretary of State, Welsh Office
1st May 1996
Sealed with the Official Seal of the Department of Agriculture for Northern Ireland this
J. Murray
Permanent Secretary
1st day of May 1996