ARTHUR C. CARANCI vs. CLIFTON H. COBB, WILLIAM J. DEVINE and PLYMOUTH-HOME NATIONAL BANK, TRUSTEE UNDER THE WILL OF WILLIAM H. COBB.
On May 12, 1965 Arthur C. Caranci filed a petition with this Court to foreclose the equity of redemption under a taking by the Town of Plymouth alleged to be dated June 13, 1962 and recorded on June 14, 1962 in Book 2940, Page 480. An amended substitute petition was filed on September 3, 1968 in which the date of the taking appears as May 4, 1962, its recording date as May 11, 1962 and the recording reference as Book 2932, Page 204. The amended petition also recites "That said premises were assigned to your petitioner by said Town of Plymouth by Instrument dated June 13, 1962 and recorded at Plymouth Registry of Deeds on June 14, 1962, Book 2940, Page 480."
After the original petition was filed, the matter was referred to a title examiner in accordance with the provisions of G. L. c. 60, § 66 to review the title in order to recommend the parties to whom notice should be sent. In the present case the examiner recommended notice to:	John K. Cobb Estate, Victorine A. Cobb Estate, William J. Thomas Estate, Frederick Thomas Estate, Estate of William H. Cobb, Estate of Jennie M. Cobb, Estate of George A. Cobb, Flora Cobb Finney, Clifton H. Cobb, married to Marion E. Cobb, Estate of Alice Howland, Estate of Sarah F. King, Carleton K. Woodard and Katie B. Wentzell. A citation was published on October 7, 14 and 21, 1965, and notice was attempted to be sent to the parties named therein.	Actual service was made only on Clifton H. Cobb. [Note 1] An answer seasonably was filed on behalf of Clifton H. Cobb and that of his wife, and he thereafter filed a further answer. Judge McPartlin allowed a motion to amend this respondent's answer which he ruled was to be considered specifications in accordance with the provisions of G. L. c. 60, § 70. After 1968 the case remained dormant until 1978-1979 when several motions to answer late and to remove defaults were filed and allowed.
A trial was held at the Land Court on October 16 and 17, 1979 at which a stenographer was appointed to record the testimony. All exhibits introduced in evidence are incorporated herein for the purpose of any appeal. All recording references are to the Plymouth County Registry of Deeds unless the context otherwise requires.
1. Prior to 1958 the property now shown on the Assessors' Maps as Lot 26 on plat 102 and Lot 15 on plat 104 was not assessed. In this time frame (see Exhibit No. 46) the local firm of Delano and Keith was engaged to prepare assessors' maps of the outlying sections of Plymouth which apparently had never been surveyed for tax purposes, Plymouth being one of the largest towns areawise in the Commonwealth.
2. As shown on plats 102 (Exhibit No. 1) and 104 (Exhibit No. 2) both lots abut the Kingston-Plymouth town line in the vicinity of Nicks Rock Road.
3. In 1958 said lots were assessed to John Kempton Cobb, heirs, c/o Arthur Caranci, and the taxes thereon were paid. The same procedure was followed in 1959 and 1960. (Exhibit No. 54C).
4. In 1961 the lots were assessed to John Kempton Cobb, William J. Thomas, Frederick Thomas, Sarah F. King, William H. Cobb, George A. Cobb, Alice Howland and Alice W. Woodward [sic]. Exhibit No. 53 gives the total 1961 taxes on the locus lots as $45.60; a portion of this, but not all, was paid in 1961.
5. From 1962 through fiscal 1979 the property continued to be assessed to John Kempton Cobb, et als, and the bills were sent to the petitioner. They have been paid in full other than those for fiscal 1979 which are outstanding in the amount of $2,897.88 plus interest and demand.	(Exhibit No. 45).
6. By instrument dated May 4, 1962 and recorded in Book 2932, Page 204, on May 11, 1962 the Collector of Taxes took for the Town of Plymouth the following described land, "Land, Plymouth and Kingston Line, being Lot 15 plat 104 and Lot 26 plat 102, containing about 154.97 acres."	The taking gave the assessed owners as John Kempton Cobb, William J. Thomas, Frederick Thomas, Sarah F. King, William H. Cobb, George A. Cobb, Alice Howland and Alice W. Woodward [sic]. It recited that the 1961 taxes remaining unpaid were in the amount of $30.40, interest to the date of taking was given as $.72 and incidental expenses and costs to the date of taking $17.00 so that the total sum for which the land was taken was $48.12. (Exhibit No. 4).
7. Thereafter the treasurer of said Town in consideration of $63.52 assigned and transferred to Arthur Caranci of 114 Westerly Road in Plymouth the tax title acquired by said Town under an instrument of taking dated May 10, 1962 and recorded in Book 2932, Page 204. The description in the assignment followed that in the taking. The assignment was dated June 13, 1962 and recorded in Book 2940, Page 480. (Exhibit No. 5).
8. Prior to the execution of the assignment the treasurer completed eight copies of the form entitled "Notice of Intention to Assign Tax Title" severally addressed to John Kempton Cobb, Sarah F. King, William J. Thomas, Frederick Thomas, Alice Howland, William H. Cobb, George A. Cobb and Alice W. Woodward [sic] (Exhibits Nos. 29D to 29K inclusive). The address of each addressee in the notice was given as "Plymouth, Mass." and the envelopes in which they were enclosed show mailing by registered mail. None of the notices was delivered, and the post office stamp on each indicates an insufficient address	(Exhibits Nos. 29A to 29C inclusive).
9. There are two deeds conveying property to John Kempton Cobb in the vicinity of Nicks Rock Road. In one the grantor was Lemuel Cobb, the deed was dated November 15, 1816 and recorded in Book 130, Page 64. This deed conveyed "a certain piece of woodland in Plymouth afores. westerly from Triangle Pond being part of my gore lot in the first great lot, containing 10 acres more or less." (Exhibit No. 8). This parcel is further described in said deed by metes and bounds.	There is a second deed from Lemuel Cobb to John Kempton Cobb dated November 28, 1818 and recorded in Book 148, Page 20. This conveyed "... forty acres of my Sixty Acre lot at Nicks Rock to be taken of at the Easterly End of my Sixty Acre lot and is in Plymouth aforesaid. Also all my Gore of land in the first great lot in said Plymouth adjoining said Sixty Acre lot and adjoining land I before sold said John K. Cobb." (Exhibit No. 10).
10. Chalk A gives the Cobb family tree with accuracy. In 1961 when the taxes were assessed for which the taking was made the members of the Cobb family to whom title had passed if we assume that John Kempton Cobb had acquired it by the deeds to which reference has been made were Alice B. Woodard, William H. Cobb, Frederick Thomas, William Thomas, George A. Cobb and Alice Howland.
11. As appears from the report of the Land Court examiner the parties entitled to notice in this case varied somewhat from those who were or should have been listed as the assessed owners as of January 1, 1961, the year for which the taking was made. A petition for probate of the estate of William H. Cobb was filed on May 19, 1961 and was not found by the examiner for the Court, perhaps because the name of the widow as listed therein varied from the name of the woman the examiner believed to be married to the decedent. As of the assessment date, however, William H. Cobb was correctly named as one of the assessed owners.
12. In order for the assessors to have learned that Alice Woodard was entitled to notice, they would have had to find the probate of her father, Edward King, since no probate for the estate of her mother, Sarah F. King, who appears in the Cobb family tree, was found. Mrs. King died after her husband, and there was no duty on the assessors to follow the devolution of her interest beyond a search for the probate records of her estate.	The absence, at least in Plymouth County, of probate records for many of the parties in the chain of title has led to the difficulties with which initially the assessors and now the Court are faced.
The petitioner claims that failure by the respondents to specify the defects in the tax taking on which they rely eliminate these from this Court's consideration other than the two raised in the amended Cobb answer and treated by Judge McPartlin as specifications. The short answer to this contention is that the case was not tried on this theory, and the respondents were allowed to introduce evidence which they claim established the invalidity of the tax title without objection by the petitioner and without limitation. In such circumstances I find and rule that it is open to this Court to consider the parties assessed, the adequacy of the description of the assessed premises and the validity of the addresses to which the notice of the intended assignment was given.
It is difficult to place the location of the premises described in the deeds in which John Kempton Cobb is named as grantee without a title examination of the surrounding parcels. Certainly no one could have quarreled with the assessors if they had elected to assess the property to John Doe or to persons unknown. See Hardy v. Jaeckle, Mass. (1976). [Note 1a] The assessors elected not to do this, however, and apparently acting on the advice of the surveyors who had prepared the new plans assessed the property to the heirs of John Kempton Cobb.	On this record it is impossible to fault the decision that locus is a part of the Cobb holdings. While it is clear that a tax assessed to one who is neither the owner nor occupant of the property is void, J. L. Hammett Co. v. Alfred Peats Co., 217 Mass. 520 , 522 (1914); Stone v. New England Box Co., 216 Mass. 8 ) 11 (1913); Desmond v. Babbitt, 117 Mass. 233 , 234 (1875), there is sufficient evidence on the record to sustain the action of the assessors in determining that the heirs of John Kempton Cobb were the owners. An expert title examiner testified at the trial on behalf of the petitioner that he was unable to establish on the records that locus once was the property of Mr. Cobb. If it were not, however, the petitioner's case would not be aided as his title rests on establishing the validity of the assessment.
The question then arises as to whether there were additional assessed owners other than those listed in the town's assessment records who should have been named as such and to whom notice should have been given. It has generally been held that if the assessment is joint, assessment to only one of the joint owners is sufficient, McManus v. Boston, 320 Mass. 585 (1947) so if one, but not all of the owners is assessed, the assessment survives attack on this ground.	Indeed G. L. c. 60, §56 authorizes the assessment, sale or taking in the name of one or more of the record owners at the date of assessment and generally provides that this is to be deemed to be in the name of the owner. In any event it is settled that the assessors need not go without the records of the Registry of Deeds and Probate in determining any parties entitled to notice. Hardy v. Jaeckle, supra. On the basis of the records as of January 1, 1961, the statutory assessment date, the determination made by the Plymouth assessors appears to have been correct. William H. Cobb died after the assessment date albeit in 1961. It also would have been possible for the assessors to find an address for Alice Woodard if they had realized Sarah F. King was the wife of Edward King and followed the title into his estate which was probated in Plymouth County, but they had no duty to follow the King clues to locate Alice Woodard and to obtain her address from the petition for probate. See Hardy v. Jaeckle, supra at 244; Fuller v. Fuller, 228 Mass. 441 , 444 (1917); Conners v. Lowell, 209 Mass. 111 , 119 (1911).
The assessors are charged with knowledge that John Kempton Cobb died testate, a resident of Plymouth, and by his will devised an undivided seven-tenths interest in the "Nick's Rock Wood Lot" to his grandchildren Lemuel Cobb and John K. Cobb and an undivided three-tenths interest therein to William J. Thomas and Frederick Thomas. The latter-named John K. Cobb was the father of William H. Cobb, and died without any probate of his estate.	Accordingly there was no reason why the Collector of Taxes should have been on notice that the William H. Cobb who died in 1961, a resident of Middleborough, was in the chain of title and thus should have been notified of the intention to assign the tax title and received a demand. It is clear from G. L. c. 60, §16, Franklin v. Metcalfe, 307 Mass. 386 (1940), that demand need be made on only one of the heirs, but it would be prudent for the Collector to select an assessed owner who may reasonably be reached. Since the Collector, no less than the assessors, is charged with notice of the records in the Registry of Probate, Conners v. Lowell, supra, it would seem that a search should have been made before mailing the notices returned by the post office for insufficient addresses.	However, even if a search were made, the Middleborough address may well have been instrumental in leading the Collector astray. [Note 3] As I have said, the burden of establishing that errors in the tax taking procedure were not substantial or misleading, the criterion of G. L. c. 60, §37, falls on the petitioner. Pass v. Seekonk, supra. A review of all the evidence does not reveal any records in the registries of which the Collector was charged with notice and which would have directed him to a proper address for at least one of the joint owners. The facts of the present case are distinguishable from Bartevian v. Cullen, 369 Mass. 819 (1976) where the deed to the record owners gave a Newton address but demand was sent only to Plymouth. In this instance I find and rule that the burden has been borne.
Land, Plymouth and Kingston Line, being Lot 15 Plat 104 and Lot 26 Plat 102, containing about 154.97 Acres.
(Exhibit No. 4). The test for the sufficiency of a description is whether it enables the owner of the property to determine that his property is the subject of the taking. Boston v. Boston Port Development Co., 308 Mass. 72 , 78 (1941). Taking into account the uncertainty of the location of the respondents' property, I think that the description in the taking was sufficient since there was no substantial or misleading error. Lowell v. Boland, 32 Mass. 300 , 302 (1951).	The reference to a plat is a customary way of drafting the shorthanded description. It obviously refers to assessors' plans; to anyone who owns undeveloped tracts of land, a description such as that used by the Plymouth assessors here would naturally have been interpreted as a reference to the Plymouth assessors' plans.
More worrisome, however, is the procedure by which the property was assessed to the Cobb heirs and the tax bills mailed to the petitioner. There has been no explanation as to why an apparent stranger was sent the bills nor any introduction of the instrument noted on the assessors' index card (Exhibit No. 54C) apparently as an indication of the reason for the assessment. So far as the record before this Court is concerned, the petitioner's interest did not materialize until the assignment of the tax title account, and even then the statute no longer permits assessment to a tax title holder. Lowell v. Marden & Murphy, 321 Mass. 597 (1947). This being so, neither should the bills have gone to him.
This Court has discretion as to redemption and may permit those having an interest to redeem upon such terms as justice and the circumstances warrant. G. L. c. 60, § 68. Johnson v. McMahon, 344 Mass. 348 (1962). At the trial it was agreed that if the Court should find the tax taking valid, evidence would be introduced as to the amounts which the parties contend should be the price of redemption. At such time evidence also is to be introduced as to the present owners of such undivided interests in said lots as were not established at the trial.
Upon all the evidence I therefore find and rule that the tax taking made by the Town of Plymouth by instrument dated May 4, 1962 and recorded in Book 2932, Page 204, is valid, that the assignment to the petitioner is not defective, and that the circumstances surrounding this case are such that the respondents are entitled to redeem.
The case is to be set down for a further hearing on the terms of redemption.
[Note 1] Gordon L. Howland also appeared but was defaulted for failure to file an answer on January 21, 1966. Subsequently the default was removed, and he was allowed to answer late. Chalk A indicates that Mr. Howland is now deceased.
[Note 1a] Mass. Adv. Sh. (1976) 2886.
[Note 2] Mass. App. Ct. Adv. Sh. (1976) 816, 819-20.
[Note 3] The experienced Land Court examiner did not locate the probate of William H. Cobb either.


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Our search exposed supplemental forms of cancer, except from your styles that we have studied in our meta evaluation that appears to be relevant with pair of genes. STRING database reviews binding interaction in between Inhibitors,Modulators,Libraries GSTP1 and GSTM1 genes, activating interaction be tween MMP2 and EGF genes, in between VEGFA and IL1B genes and involving MMP 9 and IL8 genes. The application of our machine mastering approach has substantial lighted that people pair of genes have similar association profiles and, hence, could be concerned while in the very same pathways. The genes that don’t appear within the associa tions usually do not probably correlate using the presence of a specified form of cancer. Initially, in our meta analyses, we observed the inter action between IL6 and TGFB1 genes was related to the following styles of cancer BC, CRC, GC, LC and Pc as proven in Table four.
buy TSA hdac inhibitor Though more literature search on people couldn’t validate our very steady re sults, we discovered that these interactions are associ ated to extra forms of cancer, this kind of as HNC, CRC, renal cancer, small cell lung cancer. malignant melanoma and OVCa. Moreover, regarding our even more research within the interaction concerning IL6 and TGFB1 genes on mouse designs, we now have confirmed our first success principally for BC and Computer and have observed associa tions with epithelial cancer, skin tumour, LC, OVCa and cervical cancer and HNSCC. 2nd, we found the interaction in between MMP two and EGF was linked with LC, BC and GC. Subsequently which has a even further literature search, we confirmed the association with BC osteolysis and also located new associations with EC, LC, RC and Computer.
Furthermore, in some cases, we now have observed the association of your aforementioned genes with OSCC. In this research, EGF induced MMP 1 expression that is definitely expected for form I collagen degradation. Also, MMP one can also be connected with human papillomavirus and BC. A different inhibitor TWS119 interesting interaction that was uncovered from our evaluation was concerning the VEGFA and IL1B genes that have been connected with BC and GC. Soon after proceed ing with a even more literature search, we’ve not found simi lar effects except from a single report but we have now recognized added associations with HNC, ALL, laryngeal carcinoma and MM. For MMP 9 and IL8 inter action, there was no review confirming our initial effects for BC, CRC and GC on neither people nor mouse designs.
We’ve got observed though that there was evidence for an association with nasopharyngeal carcinoma, LC and UBC. Similarly, we could not obtain any research that can assistance the interactions involving MMP 1 and MMP three and GSTP1 with GSTM1, while two research confirmed that GSTP1 and GSTM1 interac tions can be linked with BC. Indications from additional literature search on human models revealed associations for MMP 1 and MMP 3 with We’ve got then attempted to depict the several styles of cancers according towards the quantity of SNPs and genes and or gene clusters uncovered from our meta evaluation to be imply ingfully associated with particular cancer sorts. Our information in dicate that BC is correlated a lot more often than the other types of cancer the two with the amount of SNPs at the same time as together with the quantity of genes or gene clusters. This observation underlies the heterogeneity of BC, indicating that it is actually, almost certainly, not just one sickness but a spectrum of related sickness states.
Surgical elimination of the big, mixed, cystic mass during the left parieto occipital lobe resulted inside a fluid collection which measured 4. six x4. 9 cm in the operative website. There was a decrease while in the quantity of vasogenic edema and mass result as well as a lessen during the shift of your midline toward the right too being a lessen Inhibitors,Modulators,Libraries of the mass was noticed on the left lateral ventricle. Pathological examination determined higher grade glioma Frozen area diagnosis of the left occipital brain tumor was consistent with malignant glioma. Microscopically, the occipital tumor showed a substantial grade glial neoplasm. It was characterized by variably cellular, pat ternless sheets of polygonal and fusiform cells with mod erate to marked nuclear atypia, amphophilic cytoplasm, prominent nucleoli, and quite a few mitotic figures.
Irregular zones of necrosis had been surrounded by palisaded neoplastic cells. The tumor was vascular, with a lot of blood vessels lined by plump endothelial cells interspersed inside the glial element. The cellular locations in the neoplasm have been merged gradually with nearby cerebral recommended site cortex, and neuronal satellitosis was mentioned inside the transitional zone. A powerful, optimistic, glial fi brillary acidic protein stain was mentioned. Tumor grew back following surgical and adjuvant therapies as monitored by CT and MRI Two months after surgical treatment, MRI in the brain, with with out contrast, showed that, inside the area in the left posterior parietal lobe, there was a ring improving cystic spot measuring four. 5×3. 05 cm. There was vasogenic edema related to this ring improving cystic location.
There was extensive, abnormal, large signal intensity seen within the deep white matter and periventricular distributions bilat erally at the same time as within the best cerebral hemisphere. There was also elevated signal noticed inside of the thalamic region at the same time as inside selleckchem MLN9708 the inner capsule bilaterally. 4 months postsurgery, CT from the brain showed there was a prominent periventricular spot of decreased attenuation. Postoperative changes have been viewed in the left posterior parietal location. There was a fluid assortment noted. There have been focal areas of encephalomalacia while in the proper and left cerebellum. There was ex vacuo dilatation of the posterior horn of the left lateral ventricle. The prominence with the ventricles and sulci was consistent with cortical atrophy. The patient passed away shortly thereafter.
Cultured CD133 expressing cells behaved as cancer cells A fairly morphologically homogeneous tissue was obtained following the differential purification procedure, from which single cells have been obtained con taining 0. 2% CD133 favourable cells. The re latest tumor showed increased CD133 expression compared to the major tumor from your very same patient. Single cells were grown into neurospheres beneath stem cell culture strategy. The management was nor mal NIH3T3 mouse fibroblasts, grown in parallel, which ceased dividing whereas CD133 favourable cells continued to proliferate beneath the otherwise restrictive ailments of soft agar. Even though the CD133 constructive cells formed colonies in soft agar with equivalent efficiencies, the sizes of the colonies varied broadly, sug gesting they have been heterogeneous.
There was small colony formation with NIH3T3 cells. The CD133 beneficial neurospheres adhered to fibronectin in serum containing medium and spread out and extended neurite like processes. These cells expressed sure differentiation markers, such as GFAP and B Tubulin III. The cells preferred selected adhesion molecules. They grew from speedy to slow Matrigel Laminin Collagen IV Fibronectin. Cells grew more quickly with Matrigel than with any other single adhesion molecule presumably for the reason that Matrigel resembles the complex extracellular atmosphere uncovered in many tissues that incorporates several species of adhe sion molecules and development elements at the same time as other parts.
Our search exposed added sorts of cancer, except from the varieties that we have now studied in our meta examination that appears to be relevant with pair of genes. STRING database reviews binding interaction among Inhibitors,Modulators,Libraries GSTP1 and GSTM1 genes, activating interaction be tween MMP2 and EGF genes, involving VEGFA and IL1B genes and among MMP 9 and IL8 genes. The application of our machine discovering technique has large lighted that those pair of genes have equivalent association profiles and, as a result, could possibly be involved from the identical pathways. The genes that don’t seem from the associa tions never most likely correlate with all the presence of the selected variety of cancer. Very first, in our meta analyses, we observed that the inter action concerning IL6 and TGFB1 genes was linked for the following sorts of cancer BC, CRC, GC, LC and Pc as shown in Table four.
find out this here Despite the fact that more literature search on humans could not validate our hugely consistent re sults, we found that these interactions are associ ated to added styles of cancer, such as HNC, CRC, renal cancer, tiny cell lung cancer. malignant melanoma and OVCa. Moreover, relating to our more analysis over the interaction among IL6 and TGFB1 genes on mouse versions, we have now confirmed our first final results principally for BC and Computer and also have noticed associa tions with epithelial cancer, skin tumour, LC, OVCa and cervical cancer and HNSCC. 2nd, we uncovered the interaction amongst MMP 2 and EGF was related with LC, BC and GC. Subsequently that has a even further literature search, we confirmed the association with BC osteolysis and also observed new associations with EC, LC, RC and Pc.
Additionally, in some cases, we’ve got observed the association with the aforementioned genes with OSCC. Within this examine, EGF induced MMP 1 expression that is definitely expected for type I collagen degradation. Also, MMP 1 is also associated with human papillomavirus and BC. An additional selelck kinase inhibitor intriguing interaction that was uncovered from our analysis was between the VEGFA and IL1B genes that were connected with BC and GC. Right after proceed ing which has a additional literature search, we have now not uncovered simi lar outcomes except from 1 report but we’ve recognized more associations with HNC, ALL, laryngeal carcinoma and MM. For MMP 9 and IL8 inter action, there was no review confirming our original success for BC, CRC and GC on neither people nor mouse designs.
We’ve got observed though that there was proof for an association with nasopharyngeal carcinoma, LC and UBC. Similarly, we could not come across any review that can help the interactions between MMP one and MMP 3 and GSTP1 with GSTM1, although two scientific studies confirmed that GSTP1 and GSTM1 interac tions may very well be associated with BC. Indications from even further literature search on human versions unveiled associations for MMP 1 and MMP three with We have now then attempted to depict the a variety of varieties of cancers in accordance towards the variety of SNPs and genes and or gene clusters observed from our meta evaluation to get suggest ingfully connected with certain cancer styles. Our information in dicate that BC is correlated additional often than the other varieties of cancer the two using the quantity of SNPs also as with all the variety of genes or gene clusters. This observation underlies the heterogeneity of BC, indicating that it is actually, probably, not a single ailment but a spectrum of relevant ailment states.
Fibroblast like synoviocytes FLS have been cultured from your synovial tissues of RA sufferers undergoing arthroplastic surgery, as previously described, soon after getting informed consent beneath approval through the University of California, San Diego Institutional Assessment Board, and maintained in Dulbeccos Modified Eagle Medium supplemented with antibiotics, Inhibitors,Modulators,Libraries glutamine, and 10% fetal bovine serum. Pas sages 4 by eight had been utilized in experiments. Cells have been subjected to a two to three day diminished serum issue just before stimulation to mini mize baseline exercise. Secreted protein assays FLS supernatants at 24 hrs following stimulation have been assayed by ELISA for IL6, MMP1, and MMP3. Regular curves had been constructed by regression line fitting on log vs log.
Ranges of cytokines and chemokines additional info in super natants were determined by Luminex multiplex analysis from 4 parameter typical curve fits. Gene expression assays Messenger RNA for IL6, MIP1, and MMP3 have been quanti fied by true time TaqMan quantitative Polymerase Chain Reaction, making use of FLS cDNA, with GAPDH utilized being a housekeeper. Resulting threshold cycle information had been normalized to standard curves constructed from cDNA from IL1B stimulated FLS, yielding cell equiv alents. The ratio amongst the distinct cytokine and GAPDH cell equivalents is reported. Western blot FLS extracts have been ready in RIPA buffer with Full Protease Inhibitors, denatured in sample buffer and 0. one M dithiotreitol, and fractioned on Invitrogen NuPage 4 to 12% precast gels.
Following blotting to polyvinylidene fluoride membranes and blocking with 5% dry milk, blots were probed with antibodies selleck chemicals towards phospho or complete p38, JNK, Erk, or Akt, as well as with secondary anti rabbit IgG HRP. GAPDH was applied as a gel loading handle. Membranes were devel oped with Immun Star WesternC ECL substrate and imaged on the VersaDoc imaging system, employing QuantityOne software package for picture capture and densitometry. Statistical examination Information are reported as mean and typical error of your mean. Protein secretion and gene expression data in single time point experiments had been analyzed by one particular way ANOVA followed by Tukey Kramers post hoc test comparing all groups, or by Dunnetts submit hoc check com paring control to all other people, as ideal. Time program data had been analyzed by two way ANOVA followed by con trast testing. Students t check was applied to examine syner gistic results of development things and cytokines. True time qPCR information have been log transformed prior to evaluation. Benefits Result of PDGF BB and TGF B on FLS secretion of inflammatory mediators Due to the fact PDGF and TGF B are abundant from the rheumatoid synovium, their impact on cytokine induced inflammatory mediator secretion by FLS was examined.
In contrast, indicate urinary concentrations of total estrogens and estrogen metabolites measured in premenopausal Japanese American females through the luteal phase appear to become much like the Inhibitors,Modulators,Libraries imply urinary concentrations observed in Nurses Health Examine II participants who were in the luteal phase on the men strual cycle and had been largely Caucasian. Relative representation of different metabolic pathways also seem to differ across these research, with each pre and postmenopausal Japanese American gals from the present examine showing markedly decrease relative concentrations of two hydroxylated estrogen metabolites when compared to their respective counterparts. It really is not clear no matter if these differences are as a consequence of ethni city, diet or other cultural practices, or technical variables.
Numerous previous studies have investigated the asso ciations of green tea consumption with circulating or urinary estrogens. Consistent with our observation in post menopausal ladies, Wu and colleagues observed that in kinase inhibitor DMXAA postmenopausal Chinese women in Singapore, green tea consumption was linked with decrease serum es trone and estradiol. Having said that, Wu and colleagues re cently reported on the double blinded, randomized, placebo controlled intervention examine that was con ducted to investigate the result of a green tea extract containing epigallocatechin gallate on circulat ing hormones. Postmenopausal females had been randomized to acquire placebo, 400 mg EGCG, or 800 mg EGCG on a daily basis. Serum estrogens and androgens have been measured at baseline and immediately after months 1 and two in the intervention. Estrogen metabolites were not mea sured.
Investigators didn’t observe steady modifications in serum estradiol, estrone, or testosterone in any arm with the trial through the 2 month intervention but they did observe statistically major improvements in LDL choles terol and glucose connected parameters investigate this site in females provided green tea extract. Based upon these findings, the authors propose that green tea may modify cancer possibility by means of other pathways. It really should be regarded as that distinctions concerning the findings with the present review and also the randomized feeding trial could reflect distinctions within the research style or the publicity. While the present study design and style is extra susceptible than the feeding trial to confounding by unmeasured covariates, it really is also pos sible that a true effect of green tea drinking on estrogen ranges is mediated by a element not present during the EGCG isolate utilized in the trial.
In the previous cross sectional review of green tea consumption among 50 premenopausal Japanese ladies, Nagata and colleagues uncovered no association of tea with luteal plasma estradiol amounts, but did find an inverse association of green tea consumption with follicular plasma estradiol concentrations. This is steady using the null association observed concerning tea and luteal phase estrogens inside the premenopausal females studied here. The existing research is, to our knowledge, the 1st to measure a broad panel of urinary estrogen metabolites in association with intake of green tea. Findings in premenopausal ladies could suggest that green tea drinking lowers sixteen hydroxylation of estrogens. The in verse association with tea intake was observed for urinary concentrations of virtually all 16 pathway estrogen metabolites.
At the very least 200 cells were counted per culture. The outcomes had been evaluated by evaluation of variance and the Tukey check at P 0. 05, the experimental criterion currently being the significance of your response to HK treatment method in relation for the adverse manage, within the geno toxicity assay, and in relation on the favourable control once the antigenotoxicity of HK was determined as its capability Inhibitors,Modulators,Libraries to reduce the DNA harm induced by DXR. Ames test Mutagenic action was evaluated through the Salmonella microsome assay, employing the Salmonella typhimurium tester strains TA98, TA100, TA97a and TA102, kindly professional vided by Dr. B. N. Ames, with and with no metabolization, by the pre incubation system. The strains have been grown from frozen cultures overnight for 12 14 h in Oxoid Nutrient Broth No. 2.
The metabolic activation mixture, prepared from livers of Sprague Dawley rats handled together with the poly chlorinated biphenyl mixture Aroclor 1254, was purchased from Molecular Toxicology Inc. and freshly prepared prior to every single check. The metabolic activation system consisted notch inhibitor of 4% S9 fraction, 1% 0. 4 M MgCl2, 1% one. 65 M KCl, 0. 5% one M D glucose 6 phosphate disodium and 4% 0. 1 M NADP, 50% 0. 2 M phosphate buffer and 39. 5% sterile distilled water. For that determination with the mutagenic exercise, 5 unique concentrations of HK, diluted in DMSO, had been assayed. The concentrations of HK have been picked on the basis of the preliminary toxicity check. In all subsequent assays, the upper restrict with the dose selection examined was either the highest non toxic dose or even the lowest toxic dose determined in this preliminary assay.
Toxicity was detected both like a reduction in the amount of histidine revertants, or like a thinning of your auxo trophic background. The various concentrations of HK to get examined had been additional special info to 0. 5 mL of 0. 2 M phosphate buffer, or to 0. five mL of 4% S9 mixture, with 0. 1 mL of bacterial culture and after that incubated at 37 C for 20 30 min. Upcoming, 2 mL of major agar was extra plus the mixture poured on to a plate containing minimal agar. The plates have been incubated at 37 C for 48 h as well as the His revertant colonies were counted manually. All experiments were analyzed in triplicate. The results had been analyzed with all the statistical program bundle Salanal 1. 0, adopting the Bernstein et al. model. The data had been assessed by ana lysis of variance, followed by linear regression.
The mutagenic index was also calculated for every concentration examined, this becoming the typical number of revertants per plate with the check compound divided through the common number of revertants per plate with the negative control. A test alternative was viewed as mutagenic whenever a dose response connection was detected in addition to a two fold raise during the number of mutants was observed for at least one particular concentration. The normal mutagens made use of as constructive controls in experiments with out S9 mix have been NOPD for TA98 and TA97a, SA for TA100 and MMC for TA102. In experiments with S9 activation, 2 AA was utilized with TA98, TA97a and TA100 and 2 AF with TA102. DMSO served as the damaging control. The antimutagenicity assay was conducted by way of precisely the same procedure because the mutagenicity assay, except that HK was connected to known mutagens in exams with and without metabolic activation.
A minimum of 200 cells were counted per culture. The outcomes have been evaluated by examination of variance as well as Tukey check at P 0. 05, the experimental criterion staying the significance on the response to HK remedy in relation towards the damaging handle, from the geno toxicity assay, and in relation to your favourable management when the antigenotoxicity of HK was established as its capacity Inhibitors,Modulators,Libraries to reduce the DNA injury induced by DXR. Ames check Mutagenic activity was evaluated through the Salmonella microsome assay, applying the Salmonella typhimurium tester strains TA98, TA100, TA97a and TA102, kindly pro vided by Dr. B. N. Ames, with and with out metabolization, through the pre incubation process. The strains have been grown from frozen cultures overnight for 12 14 h in Oxoid Nutrient Broth No. two.
The metabolic activation mixture, prepared from livers of Sprague Dawley rats handled together with the poly chlorinated biphenyl mixture Aroclor 1254, was bought from Molecular Toxicology Inc. and freshly ready ahead of every single test. The metabolic activation process consisted selleckchem Vemurafenib of 4% S9 fraction, 1% 0. four M MgCl2, 1% one. 65 M KCl, 0. 5% 1 M D glucose 6 phosphate disodium and 4% 0. one M NADP, 50% 0. two M phosphate buffer and 39. 5% sterile distilled water. For your determination from the mutagenic activity, five unique concentrations of HK, diluted in DMSO, were assayed. The concentrations of HK had been selected within the basis of the preliminary toxicity check. In all subsequent assays, the upper limit in the dose array tested was either the highest non toxic dose or even the lowest toxic dose determined in this preliminary assay.
Toxicity was detected either as a reduction within the quantity of histidine revertants, or like a thinning of your auxo trophic background. The various concentrations of HK for being examined have been added you can look here to 0. 5 mL of 0. 2 M phosphate buffer, or to 0. 5 mL of 4% S9 mixture, with 0. one mL of bacterial culture then incubated at 37 C for twenty 30 min. Next, 2 mL of prime agar was added and the mixture poured on to a plate containing minimal agar. The plates had been incubated at 37 C for 48 h and the His revertant colonies were counted manually. All experiments have been analyzed in triplicate. The outcomes were analyzed using the statistical software package package Salanal one. 0, adopting the Bernstein et al. model. The data have been assessed by ana lysis of variance, followed by linear regression.
The mutagenic index was also calculated for each concentration examined, this being the average number of revertants per plate using the test compound divided from the typical quantity of revertants per plate with all the damaging management. A test remedy was regarded mutagenic whenever a dose response romantic relationship was detected along with a two fold increase while in the variety of mutants was observed for a minimum of 1 concentration. The typical mutagens made use of as beneficial controls in experiments without having S9 mix were NOPD for TA98 and TA97a, SA for TA100 and MMC for TA102. In experiments with S9 activation, 2 AA was employed with TA98, TA97a and TA100 and 2 AF with TA102. DMSO served as the unfavorable handle. The antimutagenicity assay was conducted by means of the identical procedure as the mutagenicity assay, except that HK was associated with acknowledged mutagens in tests with and devoid of metabolic activation.
Within a previ ous report without patient variety, treatment method with gefiti nib was not associated which has a substantial improvement in survival compared to placebo treatment. Having said that, erlo tinib has become shown to prolong survival in unselected and EGFR wild variety Inhibitors,Modulators,Libraries patients with NSCLC immediately after first line or 2nd line chemotherapy. For these good reasons, gefiti nib is administered to EGFR mutation good individuals and patients that has a larger charge of bad functionality status. In our research, gefitinib therapy showed different ad verse occasions to erlotinib therapy. In contrast on the unwanted effects of erlotinib, individuals treated with gefitinib had a drastically higher frequency of liver dysfunction. During the gefitinib group, the fee of liver dysfunction of all grades in our examine was 45. 3%, together with 19.
0% of grade one, ten. 5% of grade two, 14. 7% of grade 3, and one. 1% of grade four. Inside the erlotinib group, the rate of liver dysfunction in our review was 21. 3%, such as 8. 2% of grade one, 8. 2% of grade two, 4. 9% of grade 3, and 0% of grade four. With re spect to gefitinib kinase inhibitor canagliflozin” treatment, Maemondo et al. reported a charge of 55% of all grades of improved amounts of amino transferases, as well as price of grade three or 4 was 21. 5% inside a Japanese cohort. Mitsudomi et al. reported a price of 70. 1% of all grades as well as a charge of sixteen. 1% of grade 3 or 4. With respect to erlotinib therapy, an Asian phase III examine showed a charge of 37% for all grades of increased ranges of ALT, and also a rate of 4% of grade three or 4. Our outcomes have been just like those uncovered in previous gefitinib and erlotinib phase III clinical trials in Asian topics.
Liver dysfunctions induced by gefitinib selleck chemical had been reported in the couple of situations during which hepatotoxicity brought about by gefitinib declined when gefitinib was transformed to erlotinib. First, Kijima et al. advised the chance that CYP2D6 poly morphisms had been connected to gefitinib induced hepatotox icity. Their study described three scenarios with gefitinib associated hepatotoxicity whose genotypes have been, with phenotypes of EM, IM and EM, respectively. 2nd, Takeda et al. reported a case and recommended that liver dysfunction was attributable to a gefitinib allergy to the basis of a posi tive drug lymphocyte stimulation check. In our examine, the reduced perform of CYP2D6 was not related with an increased chance of liver dysfunction in the gefitinib cohort.
Even more examination from the unique metabolic profiles of CYP enzymes should be carried out to clarify the metabolisms of gefitinib. The DLST of gefi tinib might be regarded as of worth in some patients with gefitinib induced liver dysfunction. The in vitro metabolic process of gefitinib was investigated making use of human liver microsomes, and gefitinib metabo lized primarily by expressed CYP3A4 made a very similar range of metabolites as liver microsomes. When CYP3A4 perform was reduced or inhibited by other medication that inhibit CYP3A4, gefitinib metabolic process that will involve the formation of O desmethyl gefitinib and is deter mined from the CYP2D6 enzyme expressed in the liver was improved marginally. For that reason, the CYP2D6 enzyme is vital to the metabolism of gefitinib not just in patients with decreased CYP2D6 function, but in addition in sufferers with normal CYP2D6 perform who get other medicines associated to CYP3A4 inhibition. These patients handled with gefitinib may have extreme skin rash as a consequence of decreased metabolic process of gefitinib. We evaluated five mutated alleles, CYP2D6 1, CYP2D6 2, CYP2D6 ten, CYP2D6 14A and CYP2D6 14B, in 289 patients. The frequency of each allele was much like these reported in previous Japanese research.
Moreover, the MAPK Inhibitors,Modulators,Libraries activation inside of the host cells is associated with all the cytotoxic results exerted by V. parahaemolyticus and together with the induction of IL eight secretion by the bacterium. The various roles of MAPK signalling throughout infection with V. parahaemolyticus indicate the bacterium may use additional than one particular mechanism to sabotage regular cellular processes and disrupt host response to infection. Final results V. parahaemolyticus activates the MAPK signalling pathways in intestinal epithelial cells For a number of pathogenic bacteria modulation with the activ ity with the MAPK signalling pathway is often a important occasion within their capacity to colonise the host. The part of MAPK signalling during V. parahaemolyticus infection along with the capability on the bacteria to modulate host cell responses through this pathway hasn’t been elucidated to date.
The first aim of our examine was to examine responses of cell signalling MAPK hop over to these guys to V. parahaemolyticus. Caco 2 cells have been co incubated with WT RIMD2210633 bac teria for 15, 60 and 120 min at an MOI of ten. Anisomy cin was utilised as a beneficial handle to induce phosphorylation of every of the MAPK. Heat killed WT bacteria have been incorporated to investigate the impact of bacterial cell surface moieties on MAPK activation, within the absence of lively protein synthesis and growth. The extracted proteins had been subjected to immu noblotting examination with anti phospho JNK, phospho p38 and phospho ERK1 2 antibodies. The stripped membranes have been re probed with anti total JNK, p38, ERK1 two antibody to detect the total amount of every single MAPK protein current during the samples and also to manage for loading quantities.
JNK and p38 have been phosphorylated in cells co incubated with the WT bacteria, in comparison to samples obtained from untreated Caco 2 cells which showed no MAPK activation. Powerful activa tion of JNK and p38 was observed inhibitor b-AP15 on the two h time level, but not at earlier time points. In contrast, small or no phosphorylation of JNK and p38 was detected in cells incubated for 2 h with all the heat killed WT bacteria, indi cating the induction of activation of these two MAPK is definitely an lively procedure of V. parahaemolyticus requiring viable bacteria. The patterns of ERK activation in response to V. parahaemolyticus had been equivalent with lower phosphorylation signals detected. These scientific studies indicate that V. parahaemolyticus induces activation of the JNK, p38 and ERK MAPK signalling pathways via a mechanism requiring metabolically active bacteria.
TTSS1 of V. parahaemolyticus is responsible for activation of JNK, p38 and ERK in epithelial cells TTSS effectors of various pathogenic bacteria are proven to modify MAPK activation ranges in eukaryotic cells. As V. parahaemolyticus was capable of induce phosphorylation of p38, JNK and ERK MAPK by an energetic system, we next investigated the involvement of the TTSS of V. parahaemolyticus during the activation of these MAPK. Bacteria lacking a practical TTSS1 or even a functional TTSS2 have been constructed by deleting the cor responding vscN gene for every secretion method. Based mostly on homology to other TTSS the vscN genes are pre sumed to encode the ATPases that electrical power the secretion procedure.
The detergent inso luble cellular materials Inhibitors,Modulators,Libraries was removed by centrifugation at 14000 rpm for 15 min, along with the protein concentration of the supernatant was determined working with BioRad Dye Reagent Focus. Proteins have been resolved on four 12% steady gradient Bis Tris HCl buffered polya crylamide gels, transferred to a PVDF membrane, and blocked with 5% non extra fat milk. Membranes were probed with all the principal antibodies at dilutions indicated inside the figure legends. Certain immunoreactive bands have been detected using enhanced chemiluminescence Western Blotting Detection Reagent and Kodak X Omat film. All protein isolations and Western blots have been repeated a minimum of 3 times. PGE2 assay Cells had been seeded in twelve very well plates for 24 h. Immediately after culturing for twenty h in serum cost-free media, the cells have been taken care of with BBS for your time period of 4, 8, 10, 16, and 24 h.
Arachidonic acid was inhibitor EGFR Inhibitor extra thirty min prior to the collection of media. Culture media from each and every very well had been analyzed for PGE2 by using Biotrak Enzyme immunoassay program. All assays wherever repeat at least three times. Luciferase reporter gene assay Cells had been seeded into 12 well plates and co transfected with 250 ng of plasmid DNA containing the human COX two promoter coupled to a luciferase repor ter gene and thirty ng of plasmid containing b galactosidase applying LipofectAMINE Reagent. Cells were taken care of as described while in the figure legends. Luciferase and b galactosidase exercise were assayed applying Enhanced Luciferase Assay Kit and Galacto Light Plus Techniques, respectively. The transfections and luciferase assays have been repeated 3 times.
Immunofluorescence microscopy Computer three cells were cultured on glass coverslips. In advance of immunostaining for NF B p65 subunit, the cells had been treated either with motor vehicle, curcumin, BBS, TNF a, or perhaps a combination over at this website of curcumin and BBS or TNF a for thirty min at 37 C, fixed with 4% paraformaldehyde, permeabilized with 0. 3% Triton X 100, and incubated in blocking solu tion. Right after incubating the cells with anti NF B antiserum for 1 h at room tem perature, the cells have been washed 3 times with PBS and incubated with a goat anti rabbit IgG antibody labeled with Alexa 488. The immunostaining process was repeated no less than 3 times. Distinct immunostain ing was visualized which has a Nikon Eclipse fluorescence microscope. Electrophoretic mobility shift assay Nuclear extracts have been ready as previously described.
Oligonucleotides of which the sequence corresponding towards the AP 1 binding web page consensus sequence have been end labeled with ATP and T4 polynucleotide kinase respectively. Electrophoretic mobility shift assay reaction mixtures contained 50,000 cpm of 32P finish labeled oligonucleotide, 20 ug of nuclear protein extract, and gel shift binding buffer inside a last volume of twenty ul. Response mixtures have been resolved on 4% nondenaturing polyacrylamide gel electrophoresis at 200 V for 2 h. Gels had been dried and visualized by autoradiography. Planning of nuclear extracts and EMSA was carried out a minimal of 3 times. Intracellular Ca2 measurements Cells, grown on 25 mm glass coverslips, had been washed that has a physiological medium containing NaCl, KCl, KH2PO4, MgSO4, CaCl2, glucose, HEPES, and loaded with 2 uM Fura 2 AM for 50 min at 25 C.

matches: V. V. V. V. V. V. V. V. V. V. V.


Agreeing with State v. Gerondale, 2009AP1237-CR and 2009AP1238-CR (Wis. Ct. App Nov. 3, 2009) (unpublished), and State v. Ash, No. 2012AP381-CR (Wis. Ct. App. Aug. 15, 2012) (unpublished), that there is a conflict in § 973.01 which affects the structure of enhanced misdemeanor sentences, a court of appeals judge holds it is illegal to impose a sentence of one year of confinement followed by one year of extended supervision (ES) for a misdemeanor enhanced under § 939.62(1)(a). But while the court follows Gerondale in finding a problem in the statute, it comes up with a different approach for resolving the problem.
Griffin was convicted of a Class A misdemeanor and a Class B misdemeanor as a repeater. (¶¶1, 19). That means the usual term of imprisonment for those crimes–9 months and 90 days, respectively–is increased to two years, § 939.62(1)(a). But § 973.01(2)(c)1. says the repeater enhancer can be used to increase confinement only, not extended supervision, State v. Volk, 2002 WI App 274, ¶¶35-36, 258 Wis. 2d 584, 654 N.W.2d 24. Gerondale held that this rule seems impossible to apply to enhanced misdemeanors because, unlike classified felonies, misdemeanors are not ordinarily bifurcated, and in order to enhance a basic misdemeanor sentence to a bifurcated sentence some of the enhanced time must necessarily be used to create a supervision period. Thus, it held a misdemeanor prison sentence based on a penalty enhancer may be bifurcated no more and no less than necessary to comply with the minimum 25% extended supervision requirement of the statute.
Relying on § 973.01(2)(c)1., which provides that if the maximum term of confinement specified in § 973.01(2)(b) is increased, the total length of the bifurcated sentence is increased by the same amount, the court in this case concludes that the permissible period of ES for each crime is equal to the amount of time needed to reach the one year of confinement time imposed by the trial court, which is also the minimum required by § 973.01(2)(b). Under this formula, ES for the Class A misdemeanor can be three months (12 months minus 9 months, the ordinary maximum), and the total sentence is 15 months (12 months of confinement plus three months of ES). For the Class B misdemeanor, ES can be 275 days (365 days minus 90 days, the ordinary maximum maximum), and the total sentence can be 640 days (just over 21 months). (¶21). The court acknowledges the anomaly that the less serious Class B misdemeanor carries 275 days of possible ES time while the more serious Class A misdemeanor carries only 90 days, but it suggests it is simply following the language of the statute, which the legislature can “fine-tune” if it sees fit. (¶21 n.3). Because the circuit court’s sentences exceeded these permissible periods of ES, Griffin is entitled to resentencing. (¶22).
The result here, as in Gerondale and Ash, means a sentencing court follows some of the requirements of the statute, but not others–in particular, the rule that the term of confinement not exceed 75% of the total sentence, § 973.01(2)(b)10. At least this decision better explains its reasoning, saying the 75% rule “gives way” if it conflicts with the increase allowed by the enhancer because § 973.01(2)(b)(intro.) makes that rule subject to the increase allowed in § 973.01(2)(c)1. (¶21). Still, it results in the anomaly already noted, that the lesser crime carries the longer sentence. (NB: The footnote discussing that anomaly has what can only be a typographical error, as it suggests the circuit court could have imposed two years of confinement plus ES; but that would clearly be illegal, as it exceeds the two year maximum total sentence allowed under § 939.62(1)(a).) And, like Gerondale, it’s hard to know how to apply this opinion to other sentences. For instance, what if Griffin had been given more than 12 months of confinement. Is the maximum ES still 90 days for the Class A and 275 days for the Class B? Or, as the opinion might be suggesting by referring to the judge’s sentences in this case (¶21), is ES determined by the difference between the ordinary maximum and whatever confinement the judge imposed? For instance, if the confinement portion is 15 months, does the maximum ES go up to 180 days for the Class A and 365 days for the Class B (not all of which could be imposed, of course, because then the total sentence would exceed two years)?
Readers should also note this decision is contrary to the very recent decision in State v. Shawn Robinson, 2012AP2498-CR (Wis. Ct. App. July 23, 2013) (unpublished). The judge in that case rejected Gerondale and Ash, holding there is no conflict in § 973.01 and that a bifurcated enhanced misdemeanor sentence simply needs to comply with § 973.01’s rules: The confinement portion of the sentence be at least one year long, but not more than 75% of the total length of the sentence, and that the term of extended supervision equal at least 25% of the length of the term of confinement. The disparate results of the cases addressing enhanced misdemeanor sentences means we haven’t heard the last word on the matter. If you are grappling with this issue in the meantime, remember that imposing a jail sentence instead of a bifurcated sentence will avoid the difficulty of applying § 973.01. And, as we suggested in a previous post, there’s may be an argument that the lack of clarity in the statute means a bifurcated sentence simply can’t be imposed until the rules are clarified by the legislature.
¶15 Griffin contends that the trial court’s decision to not grant him an adjournment in order to get a lawyer was wrong because, he argues, it was contrary to its assurance that each side got one adjournment. Of course, the trial court said, as we have seen, that it was its usual policy to give each side one adjournment “for good cause.” That was not and could not be a chit that could be used at any point, even if only for delay. Given the circumstances that we have set out at length, the trial court’s refusal to derail the trial to accommodate Griffin’s last-minute request for “a public defender” was not by any stretch of the imagination an erroneous exercise of discretion. See [State v. Rhodes], 2011 WI App 145, ¶31, 337 Wis. 2d at 608, 807 N.W.2d at 8 (“‘[T]he timing of the motion is part and parcel with the consideration of whether disruption would result if the motion was granted.’”) (quoted source omitted).
Re assignment of a harsher penalty for the less serious offense: does Griffin have an equal protection argument under State v. Asfoor, 75 Wis.2d 411, 439-41, 249 N.W.2d 529 (1977) (irrational to penalize as a felony causing injury by negligent use of weapon when causing death by negligent use of weapon is penalized as misdemeanor)? Here, a Class B misdemeanor is clearly less serious than a Class A misdemeanor (whose penalty is three times as harsh), yet when the sentence is enhanced to a 1-year confinement, the formula devised by the court yields an inverted relationship (90 days ES for the Class A, 275 days ES for the Class B). Maybe that result would squeeze past Asfoor-type = pro challenge, but it does seem arbitrary nonetheless. Granted, this is a mess that calls for legislative fix — but until that bright day, some lenity-based resolution might be the way to go. Such as: make the enhanced max one year, all confinement, served in the county jail (sentences to prison must be bifurcated; barring exception, misdemeanors must be served in jail, see sec. 973.02). That outcome would retain the integrity of the underlying sentence structure, which admits of no bifurcation. And, it observes Volk’s command that enhancement be allocated solely to confinement. Yes, it’s a jerry-rigged solution, but that’s unavoidable anyway, plus it appears to do the least damage to incompatible, therefore unenforceable, statutory edicts.
The Asfoor angle in intiguing, as it does seem irrational to treat the enhanced Class B misdemeanor more harshly than the enhanced Class A misdemeanor. As for the one year in jail suggested by our post and Wm. Tyroler’s comment, that avoids the intractable bifurcation problem posited by Gerondale, Ash, and, now, Griffin. To be clear, however, that cannot mean the defendant serves a full 12 months. Jail inmates are entitled to “good time” of 25% under sec. 302.43, so any outcome purporting to commit a misdemeanor repeater to jail for the entire 12 months also creates a statutory conflict.


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Is there an association between PAWP/WBP2NL sequence, expression, and distribution in sperm cells and fertilization failures in ICSI cycles?
Mol Reprod Dev. 2018 Feb;85(2):163-170. doi: 10.1002/mrd.22950. Epub 2018 Jan 22.
Is there a relation between the time to ICSI and the reproductive outcomes?
Hum Reprod. 2018 May 1;33(5):797-806. doi: 10.1093/humrep/dey067.
Pregnancy Hypertens. 2018 Jul;13:133-137. doi: 10.1016/j.preghy.2018.06.010. Epub 2018 Jun 11.
Fuente: Andrologia. 2018 Mar;50(2). doi: 10.1111/and.12840. Epub 2017 Jul 11.
Lower endometrial receptivity in HIV-infected women receiving oocyte donation: a comorbidity of HIV infection?
Pablo Barcena, Carmen López-Fernández, Carlos García-Ochoa, Albert Obradors, Valérie Vernaeve, Jaime Gosálvez & Rita Vassena.
Amelia Rodriguez, MD, Juan José Guillén, MD, Maria Jesus López, MD, Rita Vassena, DVM, PhD, Oriol Coll, MD, PhD, Valerie Vernaeve, MD, PhD.
J Assist Reprod Genet DOI 10.1007/s10815-015-0496-0.
Vernaeve V, López MJ, Poisot F.
Syst Biol Reprod Med, Early Online: 1–9 © 2015 Taylor & Francis. DOI: 10.3109/19396368.2015.1063739.
Stem cells in reproductive medicine: ready for the patient?
Human Reproduction, Vol.30, No.9 pp. 2014–2021, 2015.
Blazquez A, Guillén JJ, Colomé C, Coll O, Vassena R, Vernaeve V.
Hum Reprod. 2014 Aug 1. pii: deu203.
Paternal age and assisted reproductive outcomes in ICSI donor oocytes: is there an effect of older fathers?
Beguería R, García D, Obradors A, Poisot F, Vassena R, Vernaeve V.
Hum Reprod. 2014 Jul 28. pii: deu189.
Vassena R, Vidal R, Coll O, Vernaeve V.
Eur J Obstet Gynecol Reprod Biol. 2014 Apr 12. pii: S0301-2115(14)00177-8. doi: 10.1016/j.ejogrb.2014.03.027.
Rodriguez A, Guillén JJ, López MJ, Vassena R, Coll O, Vernaeve V.
J Ultrasound Med. 2014 Apr;33(4):649-55. doi: 10.7863/ultra.33.4.649.
López MJ, García D, Rodríguez A, Colodrón M, Vassena R, Vernaeve V.
García D, Bautista O, Venereo L, Coll O, Vassena R, Vernaeve V.
Bodri D, Colodrón M, García D, Obradors A, Vernaeve V, Coll O.
Kawachiya S, Bodri D, Shimada N, Kato K, Takehara Y, Kato O.
Bodri D, Sunkara SK, Coomarasamy A.
Bodri D, Guillén JJ, López M, Vernaeve V, Coll O.
Bodri D, Guillén JJ, Trullenque M, Schwenn K, Esteve C, Coll O.
Galindo A, Bodri D, Guillén JJ, Colodrón M, Vernaeve V, Coll O.
Bodri D, Guillén JJ, Schwenn K, Casadesus S, Vidal R, Coll O.
Fertil Steril. 2009 Apr;91(4 Suppl):1489-92.
Bodri D, Guillén JJ, Galindo A, Mataró D, Pujol A, Coll O.
López S, Coll O, Durban M, Hernàndez S, Vidal R, Suy A, Morén C, Casademont J, Cardellach F, Mataró D, Miró O, Garrabou G.
Bodri D, Guillén JJ, Polo A, Trullenque M, Esteve C, Coll O.
Reprod Biomed Online. 2008 Aug;17(2):237-43.
Coll O, Lopez M, Hernandez S.
Curr Opin HIV AIDS. 2008 Mar;3(2):186-92.
Polo A, Vidal R, Bodri D, Coll O.
Bodri D, Colodron M, Vidal R, Galindo A, Durban M, Coll O.
Vernaeve V, Bodri D, Colodrón M, Vidal R, Durbán M, Coll O.
Coll O, López M, Vidal R, Figueras F, Suy A, Hernández S, Loncà M, Palacio M, Martínez E, Vernaeve V.
Reprod Biomed Online. 2007 Apr;14(4):488-94.
Bodri D, Vernaeve V, Guillén JJ, Vidal R, Figueras F, Coll O.
How successful is repeat testicular sperm extraction in patients with azoospermia?
Vernaeve V, Verheyen G, Goossens A, Van Steirteghem A, Devroey P, Tournaye H.
Bodri D, Vernaeve V, Figueras F, Vidal R, Guillén JJ, Coll O.
Coll O, Suy A, Figueras F, Vernaeve V, Martínez E, Mataró D, Durban M, Lonca M, Vidal R, Gatell JM.

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Derek Boogaard was a professional hockey player with the Minnesota Wild. Team doctors repeatedly prescribed Derek pain pills for injuries. He became addicted. In 2009 the NHL placed Derek into its Substance Abuse and Behavioral Health Program. Derek was checked into a rehabilitation facility and was later subject to a mandatory “Aftercare Program,” which required him to refrain from using opioids and Ambien and to submit to random drug testing. Derek joined the New York Rangers in 2010 and began asking trainers for Ambien. Derek relapsed. NHL doctors made Derek’s situation worse by violating multiple conditions of the Aftercare Program. Eventually, Derek overdosed and died. Derek’s estate sued, alleging that the NHL had failed to prevent the over-prescription of addictive medications, had breached its voluntarily undertaken duty to monitor Derek’s drug addiction, was negligent in monitoring Derek for brain trauma, and negligently permitted team doctors to inject Derek with an intramuscular analgesic. The court found some of the claims, founded on the parties’ collective bargaining agreement, were preempted by the Labor Management Relations Act and granted the NHL summary judgment. A second amended complaint was dismissed on grounds that Minnesota law applied and required a wrongful-death action to be brought by a court-appointed trustee. The Seventh Circuit affirmed, holding that the Boogaards had forfeited their claims by failing to respond to the NHL’s argument that the complaint failed to state a claim under the law of any state.
In the United States Court of Appeals For the Seventh Circuit ____________________ No. 17-2355 LEN BOOGAARD and JOANNE BOOGAARD, Personal Representatives of the Estate of DEREK BOOGAARD, Deceased, Plaintiffs-Appellants, v. NATIONAL HOCKEY LEAGUE, et al., Defendants-Appellees. ____________________ Appeal from the United States District Court for the Northern District of Illinois, Eastern Division. No. 1:13-cv-04846 — Gary Feinerman, Judge. ____________________ ARGUED JANUARY 11, 2018 — DECIDED MAY 25, 2018 ____________________ Before EASTERBROOK and BARRETT, Circuit Judges, and STADTMUELLER, District Judge. * BARRETT, Circuit Judge. Len and Joanne Boogaard appeal the dismissal of the wrongful-death action they brought as the personal representatives of the estate of their son, Derek * Of the Eastern District of Wisconsin, sitting by designation. 2 No. 17-2355 Boogaard. They devote their appeal almost entirely to arguments that would spark excitement—or fear—in the heart of a civil procedure student. There is a Hanna v. Plumer problem—whether Federal Rule of Civil Procedure 17(b)(3) controls the Boogaards’ ability to bring this suit. 380 U.S. 460 (1965). There is an Erie Railroad Co. v. Tompkins question— whether federal or state law applies if Rule 17(b)(3) does not control. 304 U.S. 64 (1938). There is a choice-of-law problem—whether Illinois, Minnesota, or New York law applies if this is a matter of state law. And there is even a relationback issue—whether, if Minnesota law applies, Federal Rule of Civil Procedure 17(a)(3)’s relation-back provision can save the Boogaards from an error that it is otherwise too late to correct. At the end of the day, however, it is an argument to which the Boogaards give short shrift that disposes of their case: forfeiture. For the reasons that follow, we agree with the district court that by failing to respond to the National Hockey League’s argument that their complaint fails to state a claim, the Boogaards forfeited any argument that it does. Their suit thus fails regardless of whether they can run the procedural gantlet of showing that they are the proper parties to bring it. I. Because we are reviewing a dismissal under Rule 12(b)(6), we treat the allegations contained in the Boogaards’ complaint as true. That does not mean, however, that we vouch for their accuracy. It means only that at this stage of the case, the Boogaards are entitled to have every factual inference drawn in their favor. In what follows, then, we re- No. 17-2355 3 count the facts as the Boogaards tell them in the complaint they filed against the National Hockey League. Derek Boogaard (“Derek”) was a professional hockey player with the National Hockey League (“NHL”). 1 He joined the NHL in 2005 as a member of the Minnesota Wild, where he remained until the summer of 2010. During his time with the Wild, team doctors repeatedly prescribed Derek with pain pills relating to various injuries and procedures. He became addicted to those pills by 2009. In September of that year, the NHL placed Derek into its Substance Abuse and Behavioral Health Program. The Program is the product of a 1996 agreement (which we’ll call the “substance abuse agreement”) between the NHL and its players’ union to create a comprehensive system for addressing substance abuse among NHL players. When a player enters the Program, he is initially permitted to receive his full NHL salary without penalty so long as he complies with the Program. If the player violates the Program’s rules, however, he receives penalties of increasing severity. Pursuant to the Program, Derek was checked into a California rehabilitation facility for in-patient treatment of his opioid and sleeping-pill addictions. Upon leaving that facility, he was subject to the NHL’s mandatory “Aftercare Program,” which required him to refrain from using opioids and Ambien and to submit to random drug testing. The NHL told Derek that his failure to follow the Aftercare Program conditions could result in his permanent suspension. 1 We call him “Derek” to distinguish him from his parents, Len and Joanne Boogaard, who represent Derek’s estate in this appeal. 4 No. 17-2355 Derek signed a contract with the New York Rangers in the summer of 2010. Before long, he began asking trainers for Ambien, leading an NHL doctor to remind him that he could not use Ambien or opioids. But Derek still relapsed. And over the following months, NHL doctors made Derek’s situation worse by violating various conditions of the Aftercare Program. They prescribed him Ambien and pain medication. They failed to impose penalties when Derek reported that he had purchased pain medications off the street over Christmas break. They again failed to impose penalties when Derek failed urine tests in January and March. And when Derek was admitted to a recovery center in California to treat opioid dependence, they allowed him to leave the facility without a chaperone. While on one such trip, Derek purchased thousands of dollars of opioids off the street; on another, he overdosed on pills and died. This litigation began two years later. Its procedural history is complicated, so we will keep it to the highlights. Derek’s estate sued the NHL and the other defendants in Illinois state court. The original complaint asserted eight claims, four of which it characterized as arising under Illinois’s Wrongful Death Act and another four under Illinois’s Survival Act. The complaint alleged that the NHL had failed to prevent the over-prescription of addictive medications to Derek, had breached its voluntarily undertaken duty to monitor and curb Derek’s drug addiction in the Program, had been negligent in monitoring Derek for brain trauma during his career, and had negligently permitted team doctors to inject Derek with an intramuscular analgesic called Toradol. No. 17-2355 5 The NHL removed the case to federal court. It argued that federal jurisdiction existed under the doctrine of complete preemption, which applies when the scope of a federal law is so broad that it essentially replaces state-law claims. The district court agreed and denied the estate’s motion to remand. It held that at least two of the claims were founded directly on rights created under the parties’ collective bargaining agreement—the claims that the NHL had breached its duties under the Program to care for Derek and address his drug addiction—and were therefore preempted by the Labor Management Relations Act. It had supplemental jurisdiction over any remaining state claims. The NHL then moved to dismiss the whole complaint for preemption and failure to state a claim. At that point, Len and Joanne Boogaard filed a first amended complaint naming themselves as the successor personal representatives of Derek’s estate. (Someone else had initially represented it.) The amended complaint invoked Minnesota’s wrongfuldeath and survival statute, although it also kept its references to Illinois law, choosing to characterize the claims as arising under both states’ statutes. The district court deemed the NHL’s still-pending motion to dismiss to be directed at the Boogaards’ first amended complaint, and the court ordered the NHL to file a supplemental memorandum in support of the motion. The NHL added a new argument for dismissal: Wrongful-death and survival actions can only be brought by a court-appointed trustee under Minnesota law, and the Boogaards were not court-appointed trustees. 2 And since the time during which 2 Minnesota law has a special statutory regime for appointing a trustee to prosecute wrongful-death and survival actions on behalf of the 6 No. 17-2355 a Minnesota court could appoint a trustee for Derek’s estate had run, this was not a problem that the Boogaards could fix. In response, the Boogaards argued that the law of Illinois, not Minnesota, determined who is entitled to bring this wrongful-death and survival action. The district court did not reach this choice-of-law problem. Instead, it granted summary judgment to the NHL on the ground that all of the Boogaards’ claims were preempted. After summary judgment, the Boogaards moved to file a second amended complaint, which added claims—still under Minnesota and Illinois wrongful-death and survival laws—that the Boogaards said were not preempted. The NHL disputed that contention, but the district court concluded that two of the new counts put forward a “theory of tort—that the NHL unreasonably harmed Boogaard—[that] is viable … and not preempted by the [Labor Management Relations Act]” and the other two “contain the seed of a viable, non-preempted claim … that the NHL actively and unreasonably harmed Boogaard by implicitly communicating that head trauma is not dangerous.” It allowed the Boogaards to file the new complaint and told the NHL that it decedent’s living spouse and next of kin. Being appointed as such a trustee is different from being appointed personal representative of the decedent or estate. Steinlage ex rel. Smith v. Mayo Clinic Rochester, 435 F.3d 913, 915–17 (8th Cir. 2006); Ortiz v. Gavenda, 590 N.W.2d 119, 124 (Minn. 1999) (stating that the appointment of a wrongful-death trustee is an exercise of the principle that “those entitled to recovery as a result of the wrongful death shall be represented by the trustee without compromise”). The Boogaards were appointed as the personal representatives of Derek’s estate, but they were never appointed as trustees for wrongfuldeath and survival actions. No. 17-2355 7 could still move to dismiss the complaint—so long as it did so on grounds other than preemption. The NHL took the district court’s suggestion. It renewed its argument, which the district court had not yet addressed, that the Boogaards’ claims could only be brought by a trustee appointed pursuant to Minnesota law. In the alternative, it argued that the new complaint did not state a claim no matter which state’s law applied. The Boogaards focused on the NHL’s argument about the Minnesota trustee requirement. 3 In addition to the choice-of-law points they had made before, they contended that Federal Rule of Civil Procedure 17(b), which governs the choice-of-law analysis in determining a party’s capacity to sue, required the court to apply Illinois law regarding who can bring a wrongful-death or survival action. The Boogaards said nothing in response to the NHL’s alternative argument that their allegations, even if true, would not entitle them to relief. The district court granted the motion to dismiss on both grounds pressed by the NHL. It held that Minnesota law applied to the action and thus required a wrongful-death or survival action to be brought by a court-appointed trustee. In the alternative, it held that the Boogaards had forfeited their claims by failing to respond to the NHL’s argument that the complaint failed to state a claim under the law of any state. This appeal followed. 3 They also responded to the NHL’s contention that the First Amendment protected it from the Boogaards’ claim that the NHL promoted violence. The parties’ arguments on that issue are not relevant to this appeal. 8 No. 17-2355 II. Before we reach the merits, we have some housekeeping to do. Every brief filed by an appellant in our court must contain a “jurisdictional statement” explaining why we have authority to decide the appeal. The Boogaards hedge in theirs. Their jurisdictional statement consists of the observation that the NHL removed the case to federal court on a theory of complete preemption. In other words, rather than assuring us that jurisdiction exists, the Boogaards essentially say “the NHL says that jurisdiction exists.” The statement does not endorse (or indeed, even acknowledge) the district court’s jurisdictional ruling, presumably because the Boogaards continue to disagree with it. Despite the Boogaards’ evident belief that jurisdiction is lacking, their brief goes on to ask us to review the merits of the district court’s decision. This is insufficient. If a party believes that we lack jurisdiction, it has an obligation to say so. We thus ordered the parties to file supplemental briefs on the jurisdictional issue so that we could discharge our obligation to determine whether we have the authority to decide this appeal. The Boogaards come clean in their supplemental brief. They argue that the Labor Management Relations Act does not completely preempt their state-law claims and that there is thus no basis for federal jurisdiction. 4 The NHL, on the other hand, maintains that the district court got the jurisdictional issue right. 4 Diversity jurisdiction does not exist, because the parties cannot satisfy the complete diversity of citizenship requirement. No. 17-2355 9 The district court did get it right. The doctrine of complete preemption “confers exclusive federal jurisdiction in certain instances where Congress intended the scope of a federal law to be so broad as to entirely replace any state-law claim.” Franciscan Skemp Healthcare, Inc. v. Central States Joint Bd. Health & Welfare Tr. Fund, 538 F.3d 594, 596 (7th Cir. 2008). In this case, § 301(a) of the Labor Management Relations Act “displace[s] entirely any state cause of action” for violation of a collective bargaining agreement. Franchise Tax Bd. of Cal. v. Constr. Laborers Vacation Tr., 463 U.S. 1, 23 (1983). It does not matter that the lawsuit styles itself as something other than a breach-of-contract action. If the suit’s claims are “founded directly on rights created by collective-bargaining agreements” or are “substantially dependent on analysis of a collective-bargaining agreement,” then § 301 governs those claims. Caterpillar Inc. v. Williams, 482 U.S. 386, 394 (1987) (citation omitted). The Boogaards’ complaint makes plain that at least two of their initial claims were based on a duty allegedly contained within the substance abuse agreement. It alleges that by administering the Program established by the substance abuse agreement, the NHL assumed the duty to curb, cure, and monitor Derek’s drug addiction. And it contends that the NHL breached that duty when it violated the procedures it had agreed to use in administering the Program. For example, the Boogaards allege that NHL doctors provided Derek with prescriptions for pain medication even though the rules of the Program forbade Derek to take any opioids and that the NHL allegedly failed to penalize Derek in accordance with the Program rules when urine tests came back positive for prohibited drugs. The complaint frequently couches its accusations of duty and breach in terms of obli- 10 No. 17-2355 gations and violations of the Program, leading unavoidably to the conclusion that the Boogaards’ claims rely on applying and interpreting the substance abuse agreement, which dictated the Program’s terms. Now, this only matters for purposes of § 301 of the Labor Management Relations Act if the substance abuse agreement is part of the collective bargaining agreement between the NHL and the NHL Players’ Association. The Boogaards say it’s not. But the collective bargaining agreement had an integration clause stating that this agreement “and any existing letter agreements between the parties that are not inconsistent with this Agreement” were the “entire understanding between the parties.” The substance abuse agreement was an existing letter agreement between the parties, and the Boogaards have pointed to no inconsistency between it and the collective bargaining agreement. Moreover, the collective bargaining agreement states that the Program will still handle certain categories of substance abuse. This reference would not make sense if the parties intended the collective bargaining agreement to supersede the substance abuse agreement. In sum, the district court correctly concluded that it had subject-matter jurisdiction over the claims asserting that the NHL breached its obligations under the substance abuse agreement. And even if that created federal question jurisdiction over only some of the claims in the complaint, the district court had supplemental jurisdiction over the rest. See 28 U.S.C. § 1367(a) (“[T]he district courts shall have supplemental jurisdiction over all other claims that are so related to claims in the action within such original jurisdiction that they form part of the same case or controversy under Article No. 17-2355 11 III … L.”). The Boogaards’ late-coming jurisdictional challenge therefore fails. III. This appeal presents a curious situation. The Boogaards devote almost their entire brief to attacking the district court’s ruling that the Minnesota trustee requirement bars their suit. But that was not the only ground on which the district court dismissed the case—it held in the alternative that the Boogaards had forfeited their claims by failing to respond to the NHL’s argument that they failed to state a claim under the law of either Minnesota or Illinois. Thus, even if the Boogaards are right about the trustee requirement, they still lose if the district court’s alternative holding stands. It is hard to fault the Boogaards for lodging a weak challenge to the district court’s forfeiture holding, because there are no strong arguments available against it. Their opener is hardly a knockout: they assert that alternative holdings should be disregarded as “mere dictum.” That contention is meritless. As an initial matter, it is well settled that we will affirm a district court’s judgment if any of several alternative holdings supports it. See, e.g., Maher v. City of Chicago, 547 F.3d 817, 821 (7th Cir. 2008). More fundamentally, the Boogaards are wrong to characterize alternative holdings as “dictum.” The rule is the exact opposite: “It is blackletter law that ‘where a decision rests on two or more grounds, none can be relegated to the category of obiter dictum.’” BRYAN A. GARNER ET AL., THE LAW OF JUDICIAL PRECEDENT 122 (2016) (citations omitted). If alternative holdings have precedential force as a matter of stare decisis, one can hardly argue that they are not independently sufficient grounds on which to affirm the judgment they support. 12 No. 17-2355 The Boogaards’ second argument is not much better. They do not—and cannot—deny that a district court may hold a claim forfeited if a plaintiff fails to respond to the substance of the defendant’s motion to dismiss. See, e.g., Kirksey v. R.J. Reynolds Tobacco Co., 168 F.3d 1039, 1043 (7th Cir. 1999); Stransky v. Cummins Engine Co., 51 F.3d 1329, 1335 (7th Cir. 1995). They argue, however, that the district court was wrong to do so in this instance. According to the Boogaards, the district court implicitly rejected a Rule 12(b)(6) argument when it permitted them to file an amended complaint adding the new claims. They insist that they were thus justified in believing that the NHL was simply regurgitating an argument that it had already lost. The record belies that contention. The district court entered summary judgment on the Boogaards’ first amended complaint solely on preemption grounds. When the NHL opposed the Boogaards’ request to file a second amended complaint, it insisted that amendment would be futile because the proposed second amended complaint likewise contained only preempted claims. That preemption point was the NHL’s only futility argument against amendment; it did not argue that the new claims also failed to state a claim under state law. The district court held that § 301 did not preempt the new claims in the proposed complaint and granted the Boogaards’ motion for leave to amend. It then invited the NHL to either answer the complaint or move to dismiss it, but the court cautioned that if the defendants chose to move to dismiss any surviving claims, “they should not do so on preemption grounds.” The Boogaards place great emphasis on the district court’s use of the word “viable” to describe the new counts No. 17-2355 13 in the second amended complaint. But in context, it is plain that the court was merely communicating its view that these counts could survive the only challenge then lodged against them: the NHL’s argument that they were preempted. The question whether the Boogaards’ allegations, if true, would entitle them to relief under state law was not before the court. And lest there be any doubt about the breadth of the court’s ruling, its express instruction that the NHL could move to dismiss on non-preemption grounds makes it even clearer that the court was not purporting to anticipatorily resolve other grounds for dismissal. If the Boogaards misunderstood the district court, the NHL’s motion to dismiss under Rule 12(b)(6) should have been a wake-up call. When the NHL moved to dismiss on grounds the Boogaards claim to believe were impliedly foreclosed, the prudent course was to clarify matters with the district court or respond to those arguments anyway. By remaining silent, the Boogaards took the risk that the district court would hold their claims forfeited. The court acted well within its authority when it did. We will not entertain the Boogaards’ alternative request that we remand to allow them to file an amended complaint. Their complaint was dismissed in the alternative for forfeiture, and amending the underlying complaint does not cure their forfeiture. Furthermore, the Boogaards have not explained in any detail what amendments they would make, which is itself reason to deny the request. Gonzalez-Koeneke v. West, 791 F.3d 801, 808–09 (7th Cir. 2015). The judgment of the district court is AFFIRMED.
Seventh Circuit affirms the dismissal of a lawsuit against the NHL by the estate of a hockey player who died of an overdose after becoming addicted to pain pills.