Our study confirmed that M. bovis infection significantly inhibits EBL growth in a dose-dependent manner. The transcription profiling **data** uncovered that M. bovis infection repressed a series of gene expressions in EBL cells, which are mainly related to metabolic process and immune response. Intriguingly, many marker genes in the PI3K-Akt-mTOR pathway showed down-regulation after M. bovis infection, suggesting that M. bovis inhibits mTOR signaling in EBL cells. Further evidence showed that M. bovis infection inhibits expression of mTOR signaling pathway marker genes in EBL cells, which are time dependent. Consistently, a previous study reported that M. bovis inhibits autophagy via a PI3K-Akt-mTOR-dependent pathway [17], which also showed that mTOR signal was stimulated at 6 h after M. bovis infection, but gradually decreased after 12 h. Data are presented as the mean ± SEM of three triplicates. ns, no significant difference. * p < 0.05, ** p < 0.01 (Student's t-test).