To investigate whether AA regulate cell growth and the cell cycle via SESN2, CMEC were treated with AA−, EAA+, AA+, AA−/si-SESN2, EAA+/SESN2 GO and AA+/SESN2 GO; and cell growth and cell cycle were Cell proliferation of CMEC treated with EAA or AA was analyzed by MTT assay, the **data** of "AA− group" was defined as "1"; (B) Cell cycle of CMEC treated with EAA or AA was analyzed by FC; (C) Expression of casein in CMEC treated with EAA or AA was analyzed by WB, the ratio value of "AA− group" was defined as "1"; (D) Expression of proteins related mTORC1 pathway, SESN2 and GCN2, and the phosphorylation ratio of mTOR and S6K1 in CMEC treated with EAA or AA was analyzed by WB, the ratio value of "AA− group" was defined as "1"; (E) Subcellular localization of mTOR in CMEC treated with EAA or AA for 6 h was analyzed by IF. Each bar represents the LSM ± SEM. In the bar charts, different superscript lowercase letters indicate significant difference (p < 0.05). Uncropped images for all Western blots can be found in Supplementary Fig. 4.