All **data** was analyzed using GraphPad Prism v7.0 (San Diego, www.graphpad.com, RRID:SCR_002798). Normal distribution was evaluated using the Shapiro-Wilk test. For comparisons between two groups, Mann-Whitney U test was used for non-Gaussian distribution samples. For multiple groups, Kruskal-Wallis test was used for non-Gaussian samples, followed by Dunn's multiple comparison post hoc test to compare between groups. Dose-response was analyzed using a nonlinear regression with a 95% confidence interval. p-Values were calculated with a 95% confidence internal, when nothing is mentioned there is no statistical significance, otherwise: *p < 0.05, **p < 0.005. Data presented was shown as mean ± SD.