RT-qPCR **data** ( Figures 7C-H) showed that Ti-treated plants have an enhanced expression of AAO3 and ABI5 when exposed to NaCl, which are critical genes in the ABA biosynthesis and ABA signaling respectively (Barrero et al., 2006;Fujita et al., 2011). This enhanced expression is clear one day after transfer to media containing NaCl and is apparently lost after 7 days of the transfer. These results indicate that a higher level of expression of genes involved in the responses to osmotic stress is critical during the early stages of the response to osmotic stress. The finding that expression of PYL4, ABI5, and AAO3 is significantly higher in seedlings grown in media containing NaCl and Ti than in those grown in media containing NaCl without Ti, confirms that Ti activates the expression of genes involved in osmotic stress, but also that to maintain a higher level of expression requires the continuous presence of Ti.