Removing all but the high quality G:C to A:T SNPs removed 51% of the remaining SNPs. The count of likely EMS-induced changes (high quality G:C to A:T) ranged from 9,668 changes in 10-2 to only 2,635 in the low coverage sequence **data** from 12-2 (Table  2). An average of 6,914 high-quality G:C to A:T SNPs were identified per sample.