FIGURE 4 .
4Estimation of the KaiC-bound fraction of the probe using a two-component analytic model of FCS. a, typical autocorrelation curves obtained after 9.4 h of incubation with samples containing TAMRA-KaiB-His 6 and KaiB (Fig. 3a) or the standard Kai protein mixture (Fig. 3c) are indicated using open and filled circles, respectively. The autocorrelation function of TAMRA-KaiB-His 6 mixed with only KaiB was fit with a one-component analytic model, and the diffusion time was estimated to be 482.5 Ϯ 16.6 s (blue fitting curve). The autocorrelation function of TAMRA-KaiB-His 6 in the standard mixture was fit with a two-component analytic model by fixing the diffusion time of the free fraction at 482.5 s (red fitting curve). b, circadian oscillation of the ratios of KaiB-bound KaiC complex. Ratios of the complex were computed using the same **data** set in Fig. 3c.
