First, in our analysis, Msn2, Sok2, and HOG TFs (Hot1 and Sko1) form a TF cluster ( Figure 4A), and the HeRS scores between Sok2 and HOG kinase are greater than that of the Msn2 and HOG kinase. Since Msn2 is the substrate of Hog1 [22], the above **data** indicates close relationship between Sok2 and HOG pathway. Plus, in meiosis and mitosis, Sok2 associates with Msn2/4, and they are co-regulated in the cAMP-dependent protein kinase signal transduction pathway [23]. These observations suggest that Sok2 and Msn2/4 may also be co-regulated in HOG pathway. Second, a recent comprehensive phenotypic analysis has found that the deletion of Sok2 caused a decrease in the hyperosmotic stress resistance of cells [24]. This provides direct evidence that Sok2 is involved in HOG pathway.