Figure 3 .
3The uORFs can affect the expression of mouse mu opioid receptor. (A) Structures of fused constructs. Each construct contains mouse MOR promoter. The coding regions were fused in-frame to the GFP gene except in the case of the negative control vector (pGL Basic). (B) Flow cytometry analysis of mouse MOR fusion constructs in transiently transfected mammalian cells. EGFP mean value (fluorescence intensity) in HEK293 cells was measured by flow cytometry. Mutation of uAUGs greatly increased the level of GFP expression. The **data** represent an average of three independent experiments. (C) Radioligand binding assay.
