Keeping in view the economical significance of LPAI there is a dire need to enhance resistance against LPAI in broilers to overcome the adverse effects of this disease. As the previous literature suggests that the administration of some vitamins and unsaturated fatty acids may enhance resistance to certain diseases by increasing immunity (De-Rosa et al., 2015) therefore the present study was based upon the hypothesis that administration of vitamin E and omega 3 separately and in combination may improve resistance to avian influenza infection in broiler chicken. As there is some **data** available about the immunostimulatory effects of vitamin E and omega fatty acids against various poultry diseases therefore this study first time determined the effects of vitamin E and omega 3 against LPAI.