Analysis of the RNA-seq **data** of Ti-treated plants revealed enriched GO categories related to defense responses to bacteria, fungus, and viruses, as well as programmed cell death (Figure 4). Therefore, we decided to explore in more detail the effect of Ti treatment on the transcript level of genes involved in SA biosynthesis and signaling routes. Our **data** showed that several SA-related molecular components were responsive to Ti treatment in both shoot and roots. We found that several NBS-LRR receptors are upregulated in Ti-treated roots but only a few in shoots ( Figure 8A). PAD4, which is part of the SA regulatory module is upregulated in roots and lightly in shoots. The positive regulators of SA biosynthesis WRKY54, WRKY70, and CBP60g, are upregulated in roots, and WRKY54 only in shoots. The negative regulators of SA biosynthesis CAMTA2, CAMTA3, and CAMTA5 are also upregulated in roots with only CAMTA3 faintly upregulated in shoots. Interestingly no SA biosynthesis genes were found upregulated either in roots or shoots, suggesting that Ti might preactivate positive regulators of SA biosynthesis but at the same time negative regulators to prevent an undesirable high level of SA that could affect plant growth. NPR1 and NPR3, which regulate the SA response pathway are upregulated in roots but only NPR3 is upregulated in shoots. Downstream of SA perception by NPR1, pathogen-related genes, and WRKYs transcription factors are activated directly by TGA2, TGA5, and TGA6, which ultimately activate the global plant immune response known as SAR. TGA2 and TGA6 are upregulated in shoots while PR1 and WES1 are upregulated in the shoot. The cell death positive regulator SBB1 is upregulated in both roots and shoots whereas the mRNA exporter DRH1 which is needed to initiate cell death, is not. We also found the receptors BAK1 and BKK1, negative regulators of SAdependent cell death, are down-regulated in roots but not in shoots ( Figure 8B). These results altogether suggest that Ti might induce a molecular priming-like response against pathogens.