Therefore, all results indicate that Roy (1) is the most promising phytochemical to be further explored and as a lead compound to further develop new therapeutic strategies against GB.  (1), which had the highest antiproliferative/cytotoxic effect against GB cells, was conjugated with a fluorescent probe to assess its biodistribution in the cells. Using fluorescence and confocal microscopy and acquired **data** and image analysis, we observed a pronounced fluorescence in the cells treated with both concentrations of Roy-BODIPY (12), 6.25 µg/mL ( Figure 5B,D) and 25 µg/mL ( Figure 5A,C). Moreover, Roy-BODIPY (12) accumulated in the cytoplasm of cells instead of the nucleus (pointed out with N in each image) ( Figure 5). Using confocal microscopy, a distinctive accumulation in cytoplasmic vesicles was visible (marked with an arrow) ( Figure 5C,D). Therefore, the increased uptake of Roy (1) in cytoplasmic vesicles supported the finding of the higher antiproliferative/cytotoxic activity of this phytochemical ( Figure 5). Furthermore, a possible explanation for these results could be the fact that this compound may target the mitochondria and induce the mitochondriadependent intrinsic pathway of apoptosis. Further studies should be performed to unveil the exact mechanism of action of Roy (1).