To evaluate the phenotypic relationships among accessions, the minimum distances between all pairs of individual accessions were constructed by plotting on a dendrogram ( Figure 2). To gain a better understanding of the overall diversity of nightshade accessions, the **data** were analyzed using cluster analysis, which revealed the similarities between the genotypes. SRetrflx, SABGA and Nshad9 had the lowest dissimilarity index (0.00) ( Table 5)