In this study, we applied FTIR microspectroscopy to define plant tissue within cassava leaf sections and used this information to detect variations between control and those treated cassava stakes by B. subtilis. In particular, we demonstrate the use of FTIR microspectroscopy technique combined with multivariate **data** analysis to discriminate major tissue types in the cassava leaf, and demonstrate how this information enables us to track biochemical changes in cassava leaf tissue related to enhancement ofgrowth and development of cassava.