EBL cells after M. bovis treatment with 50 MOI were collected for total RNA extraction and transcriptome analysis. In brief, cellular total RNAs were isolated using the Trizol reagent (Takara, Dalian, China). Qualified total RNA was further purified by an RNeasy mini kit (Qiagen, GmBH, Hilden, Germany). The mRNAs were enriched using the Oligo(dT) beads and then were fragmented and reverse transcribed into cDNA. The cDNA Library quality was checked using a Bioanalyzer2100 (Agilent, Santa Clara, CA, USA). The cDNAs were purified with QiaQuick PCR extraction kit (Qiagen, GmBH, Germany), end repaired, poly(A) added and ligated to Illumina sequencing adapters. The ligation products were size selected by agarose gel electrophoresis, PCR amplified and sequenced using Illumina HiSeq 2500 by Gene Denovo Biotechnology (Guangzhou, China). The random reads were mapped to nucleotide database Bos taurus (GCF_002263795.1_ARS-UCD1.2_genomic). The gene expression level was calculated by using the RPKM method (Reads Per kb per Million reads). FDR (False-Discovery Rate) is a method to determine the threshold of p-value. All **data** analyses were supported by Denovo Biotechnology (Guangzhou, China). Data were submitted to the gene expression omnibus (GEO) archive. The accession number is SUB12077437.