For the modeling, **data** analysis and experimental design, the software (Design Expert trail version 8.0.6) was applied. Response surface analysis Box-Behnken Design (BBD) with four independent variables hydrolysis time (X 1 ), pH (X 2 ), temperature (X 3 ), and an enzyme dosage (X 4 ) by setting the maximum response value Y (ACE inhibitory activity %) as the primary goal. Center points for RSM design were obtained from previously studied single-factor experiments. The center point value was set according to results of the single-factor experiments studied previously expressed in the (S.2). The proposed model was expressed in the form of 2D and 3D counter plots. Furthermore, the model was validated by performing an additional experiment (n = 3) was conducted to check the model validity and significance, respectively.