only and activated T cells post - washed with fresh media after pre - treated with chrysophanol for 30 min or 1 h. As shown in Figure 3E , pre - treatment with chrysophanol for 30 min and 1 h of T cells exerted significant decrease of mRNA level of il2 but removal of chrysphanol after pre - treatment did not show any attenuation in mRNA level of il2 . Interestingly , decrease of mRNA level of il2 is dependent on the time of pre - treatment with chrysophanol , meaning that pre - treatment with chrysophanol for 1 h exhibited more inhibitory effect on T cell activation than pre - treatment for 30 min . These data imply that T cell activity is irreversibly restrained by pretreatment with chrysophanol in Jurkat T cells stimulated by antibodies against CD3 / CD28 and SEE - pulsed Raji B cells .