AQP2 Interaction with 14-3-3 Is Regulated by AVP-To examine if the AQP2 and 14-3-3 interaction was regulated by AVP, mpkCCD 14 cells were cultured in dDAVP for 3 days (to increase AQP2 levels), followed by acute stimulation with dDAVP (see "Experimental Procedures"). Following 20 min dDAVP stimulation, the interaction between AQP2 and 14-3-3 was enhanced, with a greater quantity of 14-3-3 immunoisolated using an AQP2 antibody, or conversely, a greater quantity of AQP2 immunoisolated using a 14-3-3 antibody (Fig. 5A). Quantification **data** from 3 individual immunoprecipitations is shown in Fig. 5, B and C.