We have demonstrated that hetero-regulators in the same signaling pathway tend to have a high HeRS score, and HeR modules map well to known pathways. Conversely, a high HeRS score can indicate a co-pathway relationship of the corresponding hetero-regulators. Here, we take TF Sok2 as an example to illustrate how to predict gene functions based on HeR modules. Sok2 forms a HeR module with known HOG TFs and HOG kinase ( Figure 4A, Table 1), and it binds to many genes in the HOG pathway ( Figure 4B). These **data** predicts Sok2 as a potential TF in the HOG pathway. Although no previous study has reported Sok2's function in HOG pathway, there is indirect evidence to support this claim.