Picrosirius Red staining **data** showed that the CPC/alginate films had accumulated the less amount of collagen on day 3 but increased by day 7 with a decrease noted on day 14. The CPC/alginate films also had produced a lesser amount of collagen when compared with the other two coatings (Fig. 8) In contrast, CPC/alginate/chitosan films produced an initial higher amount of collagen which was maintained through day 14. CPC/alginate/chitosan/HNT films produced the most collagen by day 3 when compared against the other scaffolds. The levels of collagen remained relatively similar on days 3 and 7 and decreased in amount by day 14. The cumulative supports the observation that cells on all substrates produced a base organic extracellular matrix.