To further characterize the biological processes that are activated in response to Ti treatment, we manually analyzed DEGs induced at three days of Ti treatment and performed a Gene Ontology (GO) enrichment analysis of DEGs induced 10 days after the treatment with Ti. In the case three days after the treatment, we found that two of the four upregulated genes in roots of Ti-treated plants (CA1 and RBCS2B) are targets of OXIDATIVE STRESS 2 (OXS2). OXS2 is a tandem zinc finger transcription factor previously identified to play a crucial role in salt tolerance in Arabidopsis . The third gene, AT3G48200, encodes a transmembrane protein which knockout mutant is tolerant to heat stress and insensitive to abscisic acid (ABA), a phytohormone playing an important role in osmotic stress responses (Luhua et al., 2013). The fourth Ti-responsive gene at three days of Ti treatment, AT5G51585, is still uncharacterized. These **data** suggest that Ti treatment might initially induce only genes involved in tolerance to osmotic stress.