Expression profiles comparing relative mRNA and protein abundance Figure 5 Expression profiles comparing relative mRNA and protein abundance. The panels summarize mRNA and protein abundance **data** for individual P. falciparum genes, with PlasmoDB accession numbers indicated below the gene names. Both the relative mRNA and protein abundance levels determined in this study for four schizont-stage time points (TPs) are based on the same parasite samples from a single large-scale in vitro culture and are indicated by gray and colored lines (see panel legends), respectively. In addition, the previously published transcript dynamics across the whole intra-erythrocytic life cycle [2] are included as black lines, with the black dots representing the corresponding raw data. All abundance profiles were mean-centered around zero based on the **data** points between 34 and 46 hours post-invasion. Asterisks in the panel legends denote cases for which analysis of variance (ANOVA) P < 0.01 and maximum fold change >1.4×. The insert in the eukaryotic initiation factor (eIF)5A panel depicts actual two-dimensional gel images to provide an approximate impression of absolute protein abundance levels between the three eIF5A isoforms.   (Figure 6d). This pattern mirrors the DIGE expression profiles of spots 2 and 3 ( Figure 5), whereas the differentially expressed spot 1 -because of its low absolute expression level (see inset in Figure 5) -presumably does not contribute significantly to the overall changes in protein abundance.