In conclusion, the **data** presented in the present study could contribute to a better understanding of the biological processes involved in a possible loss of infectivity of L. amazonensis when submitted to in vitro cultures over a long period of time, as described for other Leishmania species. Furthermore, the identified proteins presenting a significant decrease in their protein content during cultivation, including the hypothetical, should be evaluated in future studies, including vaccine candidates and/or immunotherapeutic targets against leishmaniasis. Additional studies are warranted in an attempt to address the major concern that identified proteins are indeed involved in the possible loss of virulence in the parasites cultured over long periods of time.  
