( D ) WT and ( E ) p23KD HeLa cells were treated with 40 µg / mL of cycloheximide ( CHX ) for 6 h in the presence or absence of 40 µM CQ for 12 h ( 6 h pre - treatment and then co - treated with CHX for another 6 h ) . The degradation of AHR in both cell lines was inhibited by CQ . For ( D , E ) , the below images are representative of the replicate data ( means ± SD , n = 3 ) . Conditions with no addition as no treatment ( NT ) were arbitrarily set as one ( with no error bar ) for data normalization . Multiple t - tests corrected with the Holm - Sidak method for multiple comparisons were performed to determine statistical significance .