300 seedlings were germinated in a hydroponic system using liquid MS medium as reported previously by Alatorre-Cobos et al. (2014) with slight modifications. In brief, plants were sown in a 0.75 mm by 0.75 mm opening size mesh touching liquid MS medium. Five days after germination, seedlings were transferred to MS medium agar plates supplemented with 2 mM Ti or only MS medium as the control reference and grown for three or ten more days in the conditions described above. Afterward, shoots and roots were collected separately and dried at 65°C for 5 days. Roots and shoots from a total of 1,500 plantlets per treatment were collected and pooled for each replicate. The elemental profile analysis was conducted for 19 elements in the standardized pipeline of the Baxter laboratory at the Donald Danforth Plant Science Center as described by Ziegler et al. (2013) The content of each sample was obtained as mg of the analyte/kg of the sample and used to calculate relative changes of each element; **data** is reported as a percentage change.