Statistical analyses were performed using IBM SPSS Statistics version 22 ( IBM , Armonk , NY , USA ) . The significant differences in expression levels of SfVg and SfVgR in different developmental stages and tissues were analyzed with a one - way ANOVA analysis followed by Tukey 's test ( α = 0.05 ) . The significant differences in interference efficiency and reproductive parameters in the RNAi experiment were assessed by Student 's t - test ( * p < 0.05 , * * p < 0.01 , * * * p < 0.001 ) . All data were shown as means ± standard errors ( SE ) .  