Mixed methods were used for **data** collection. The qualitative **data** collection was carried out first, making use of semi-structured interviews and focus group discussions (FGDs). A total of 26 semi-structured interviews were conducted with eight livestock owners, four shepherds, and a further five with livestock owners' wives, and five with shepherd wives. A One Health approach was applied by jointly discussing animal and public health workers and their respective roles in knowledge production and transfer to pastoral communities. Four semi-structured interviews were conducted with health workers, one with a male nurse, another with a physician and two with veterinarians. Eight FGD were conducted with six people per group. Three focus groups were organised with sedentary livestock owners, whilst two discussion groups involved transhumant pastoralists, and participants of the three other FGD comprised respectively herdsmen, livestock owners' wives and herdsmen' wives. Printed pictures of hygromas and orchitis in ruminants were used during those interviews to structure the discussion on symptoms of brucellosis in animals. All interviews were recorded using a digital voice recorder. For the quantitative **data** collection, 320 questionnaires were completed. Interviews and questionnaires were prepared and conducted in Malinké (the local language commonly called Dioula), by a trained research assistant under a close supervision of the main researcher to ensure the accuracy of the translation of each discussion to French. The answers were then analysed and translated from French to English in this manuscript. Names and expressions from pastoral communities in local languages used to identify brucellosis symptoms were called Popular Nosological Entities (PNE). During the various interviews, brucellosis in animals was presented by the interviewer through its clinical symptoms such as hygromas, orchitis, drop in milk production and spontaneous abortions. Participants were also asked if they were familiar with the term brucellosis to describe this disease during the quantitative survey.