F 2 ,
236 = 1.760, p = 0.1865). C,D: n = 5-9 trials of 40 flies. (E,F) Spontaneous locomotor activity presented as violin plots of the mean number of beam crossings. (E) Age and genotype affect locomotor activity in males (Kruskal-Wallis: p = 0.0004), and male nlg3 Def1 had increased locomotor activity compared to Cs at 7-10 days old but not at 3-4 days old (Dunn's post hoc: p = 0.0032). (F) In females a similar trend was observed (Kruskal-Wallis: p = 0.0367), where nlg3 Def1 7-10 days old had higher activity than Cs (Dunn's post hoc: p = 0.0806). E,F: n = 15 individual flies. Dotted horizontal lines represents 25-75% quartiles, solid horizontal line displays the median. Those are not visible when 75% of the **data** = 0. Lighter colors represent 3-4 day-old flies. Darker colors represent 7-10 day-old flies.
