The **data** from the histochemical analysis (Alcian Blue and Picrosirius Red staining) was plotted as a graph of means of **data** points per sample/type/day for two experimental trials (n = 3 samples per trial) and the samples were independent of each other. Inferential statistics, such as ANOVA, could not be used because of the limited sample size; hence, a simple descriptive statistic was used for the interpretation of the results. The error bars were calculated as the percent error.