Fig. 2 .
2Kinetic measurement of caspase-3 activity in cells treated with ADM. Cells were treated with 5 µM ADM for 24 h, after which cell lysates were assayed with or without the caspase-3 inhibitor Ac-DMQD-CHO. Cell lysates were incubated at 37°C for 1 h, and the amount of AMC released was measured by cleavage of the fluorogenic substrate Ac-DEVD-MCA (100 µM). The vertical axis represents relative values compared to untreated cells. The **data** represent the mean±SD of three independent experiments. Statistical significance was determined by Student's t test, as compared to the value in PANC-1 cells without treatment, * P<0.05, * * P<0.02. Ac-DMQD-CHO, acetyl-Laspartyl-L-methionyl-L-glutaminyl-L-aspartyl-aldehyde; ADM, adriamycin; AMC, amino-4-methylcoumarin; Ac-DEVD-MCA, acetyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid-α (4-methylcoumaryl-7-amide).
