The collagen morphology of hypertrophic scars was analyzed by Masson's trichrome staining. The initial hypertrophic scar showed dense, disordered collagen fibers and large numbers of fibroblasts. At week 8, hypertrophic scars treated with Shikonin or Silicone gel showed loose, parallel bundles of collagen fibers and decreased numbers of fibroblasts in the dermis, compared with the scars treated with saline (Figures 5A-D). Taken together, these **data** indicated that Shikonin reduced the scar area, decreased the epidermal and dermal thickness and improved the final outcome of the hypertrophic scars.