PEDF is localized in human testicular biopsy tissue, particularly the extracellular matrix and the cells of the tubular wall [48]. PEDF has been reported to be responsible for the development and maintenance of the avascular nature of seminiferous tubules, which are important for orderly spermatogenesis [48]. Our **data** show that PAL increased CYP-induced downregulation of testicular PEDF, which indicates that PAL contributes to the avascular nature of seminiferous tubules and spermatogenesis.