Secondary metabolites (essential oils) from sand-dune plants induce cytotoxic effects in cancer cells Skip to main content Home Research Outputs People Faculties, Schools & Groups Research Projects Research Areas Global Challenges Research Fund QR funding Global Development Research The Ideas Fund Research Centres/Groups Advanced Material and Acoustics Analytical Chemistry Measurement Andrew Marvell Centre for Medieval and Early Modern Studies Aura Better Policing, Safer Communties Biomedical Engineering Research Group Biomedical Sciences Research Group Bioscience Education Blaydes Maritime Centre Catastrophic Flows Centre for Applied Research and Evaluation Centre for Applied Research in the Arts Centre for British Politics Centre for City and Regional Studies Centre for Criminology and Criminal Justice Centre for European Union Studies Centre for Gender Studies Centre for Government Centre for Health and Clinical Neuroscience Centre for Idealism and the New Liberalism Centre for Legislative Studies Centre for Mathematical Sciences Centre for Performance, Technology and Aesthetics Centre for Research into Embodied Subjectivity Centre for Research into the Older Workforce Centre for Security Studies Centre for Spirituality Studies Centre for Sustainable Energy Techonologies Centre for Systems Studies Centre for Textual and Cultural Analysis Centre for War Studies Chemistry for Health Chemistry for Smart Materials Chemistry for Sustainability Cognition and Development Cognitive and Clinical Neuroscience Collection: COVID-19 Collection: City of Culture Cultures of Incarcertion Centre Dementia Hull Dependable Intelligent Systems Digital Centre E.A. 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Home Outputs Authors Ellie Beeby Mariana Magalhães Juliana Poças Thomas Collins Marco Lemos Lillian Barros Isabel Ferreira Célia Cabral Dr Isabel Monteiro dos Santos Pires I.Pires@hull.ac.uk Senior Lecturer in Biomedical Science/ Head of the Hypoxia and Tumour Microenvironment group Abstract Ethnopharmacological relevance: Despite advances in modern therapeutic strategies, cancer remains the second leading cause of death worldwide. Therefore, there is a constant need to develop more efficient anticancer targeting strategies. The anticancer therapeutic proprieties of medicinal plants and their bioactive compounds have been reported for several years, making natural extracts and/or compounds derived from these a promising source of novel anticancer agents. Sand dune plants are subjected to severe environmental stresses, leading to the development of adaptations, including the production of secondary metabolites with a wide range of bioactivities, such as: anti-inflammatory, analgesic, antiseptic, hypoglycaemic, hypotensive, antinociceptive, antioxidant and anticancer. Aim of the study: The anticancer potential of sand dune plants remains under-investigated, so this research describes the characterisation of the composition of bioactive EOs from sand-dune plants of Peniche (Portugal), and assessment of their activity in vitro and potential mechanism of action. Materials and Methods: EOs were extracted from six sand-dune species of plants from Peniche sand dunes: Crithmum maritimum L., Seseli tortuosum L., Artemisia campestris subsp. maritima (DC.) Arcang., Juniperus phoenicea var. turbinata (Guss.) Parl., Otanthus maritimus (L.) Hoffmanns. & Link, and Eryngium maritimum L.. EOs composition was fully characterised chemically using Gas Chromatography-Mass Spectrometry (GC-MS). The assessment of anticancer activity and mechanism of action was performed in vitro using breast and colorectal cancer 2D and 3D spheroid cell line models, through cell proliferation assay, western blotting analysis, and cell cycle analysis. Results: EOs from the majority of the species tested (S. tortuosum, A. campestris subsp. maritima, O. maritimus, and E. maritimum) were mainly composed by hydrocarbon compounds (sequisterpenes and monoterpenes), showing antiproliferative activity in both 2D and 3D models. EO extracted from S. tortuosum and O. maritimus were identified as having the lowest IC50 values for both cell lines when compared with the other species tested. Furthermore, this antiproliferative activity was associated with increased p21 expression and induction of apoptosis. Conclusions: The present study suggests that EOs extracted from S. tortuosum and O. maritimus present promising cytotoxic properties. Further evaluation of the extracts and their key components as potential anticancer agents should therefore be explored. Citation Beeby, E., Magalhães, M., Poças, J., Collins, T., Lemos, M., Barros, L., …Pires, I. M. (2020). Secondary metabolites (essential oils) from sand-dune plants induce cytotoxic effects in cancer cells. Journal of Ethnopharmacology, 258, Article 112803. https://doi.org/10.1016/j.jep.2020.112803 Journal Article Type Article Acceptance Date Mar 25, 2020 Online Publication Date Apr 3, 2020 Publication Date Aug 10, 2020 Deposit Date Mar 30, 2020 Publicly Available Date Apr 4, 2021 Journal Journal of Ethnopharmacology Print ISSN 0378-8741 Electronic ISSN 1872-7573 Publisher Elsevier Peer Reviewed Peer Reviewed Volume 258 Article Number 112803 DOI https://doi.org/10.1016/j.jep.2020.112803 Keywords Sand-dune plants; Essential oils; Natural products; Antitumoral properties Public URL https://hull-repository.worktribe.com/output/3486071 Publisher URL https://www.sciencedirect.com/science/article/abs/pii/S0378874119349633 Files Article (9.8 Mb) PDF Download Preview Publisher Licence URL https://creativecommons.org/licenses/by-nc-nd/4.0/ Copyright Statement ©2020. 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