Vaccinia virus assembly and motility Study at UCC | Current Students | Research | Staff | Teaching & Learning | Visitors | Alumni | About UCC UCC | UCC Library | IRIS Vaccinia virus assembly and motility CORA Home → UCC Theses → Doctoral Theses → View Item JavaScript is disabled for your browser. Some features of this site may not work without it. Files in this item Name: CudmoreS_PhDThesi ... Size: 39.94Mb Format: PDF Description: Full Text E-thesis View/Open Vaccinia virus assembly and motility Cudmore, Sally Date: 1996 Copyright: © 1996, Sally Cudmore. Copyright information: http://creativecommons.org/licenses/by-nc-nd/3.0/ Related: http://library.ucc.ie/record=b1255996~S0 Full text restriction information: No embargo required Citation: Cudmore, S. 1996. Vaccinia virus assembly and motility. PhD Thesis, University College Cork. Supervisor(s): Cotter, Thomas G.; Sheehan, David; Griffiths, Gareth Abstract: Vaccinia virus, the prototype member of the orthopoxviruses, is the largest and the most complex virus known. After replication of its genome and expression of the viral proteins, vaccinia undergoes a complicated assembly process which produces two distinct infectious forms. The first of these, the intracellular mature virus (IMV), develops from the immature virion (IV) after packaging of the genome and cleavage of the core proteins. During the transition of the IV to the IMV, a new core structure develops in the centre of the virion, concomitantly with the appearance of spike-like structures which extend between this core and the surrounding membranes of the IMV. I describe the characterization of p39 (gene A4L) which is hypothesized to be one component of these spikes. p39 is a core protein, but has strong associations with the membranes surrounding the IMV, possibly due to an interaction with p21 (A17L). Due to its location between the core and the membranes of the IMV, p39 is ideally situated to act as a matrix-like linker protein and may play a role in the formation of the core during the transition of the IV to the IMV. The IMV is subsequently wrapped by a membrane cisterna derived from the trans Golgi network, to form the intracellular enveloped virus (IEV). I show that the IEV can co-opt the actin cytoskeleton of the host cell in order to induce the formation of actin tails which extend from one side of the virion. These actin tails propel the virus particle, both intra- and intercellularly, at speeds of up to 2.8µm/min. On reaching the plasma membrane, the virus particles project out from the cell surface at the tip of virally induced microvilli. The outer membrane of the IEV is thought to fuse with the plasma membrane at the tip of these projections, thus exposing the second infectious form of vaccinia. This is thought to be the means by which the cell-associated enveloped virus is presented to neighbouring cells, thereby facilitating the direct cell-to-cell spread of virus particles. Show full item record This item appears in the following Collection(s) Doctoral Theses [1622] College of Science, Engineering and Food Science - Doctoral Theses [1000] Biochemistry and Cell Biology - Doctoral Theses [84] Except where otherwise noted, this item's license is described as © 1996, Sally Cudmore. Search CORA Search CORA This Collection Browse All of CORA Communities A-Z Authors Titles Keyword / Subject Date UCC Theses This Collection Authors Titles Keyword / Subject Date My Account Login CORA information About CORA How to submit your research How to submit your UCC thesis Benefits of archiving in CORA Copyright Statistics Most Popular Items Statistics by Country Most Popular Authors For information or help contact the IR Manager at UCC Library, email:cora@ucc.ie or Tel. +353 21 420 5109. Feedback Password Login Theme by Dspace Software Copyright © 2002-2008 The DSpace Foundation This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement