CD24, a signal-transducing molecule expressed on human B cells, is a major surface antigen on small cell lung carcinomas. — MRC Weatherall Institute of Molecular Medicine Cookies on this website We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings. 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Cell lines derived from human small cell carcinoma of the lung express high levels of a surface polypeptide termed the cluster-w4 antigen, which was previously identified as a potential target for toxin-based immunotherapy of lung cancer. We have cloned a complementary DNA encoding the cluster-w4 antigen from COS-1 fibroblasts transfected with a SW2 small cell carcinoma library, by panning with a mixture of the cluster-w4-specific monoclonal antibodies SWA11, SWA21, and SWA22. The sequence of the cluster-w4 complementary DNA encodes an unusually short (80-amino acid) protein identical to that recently reported for the leukocyte activation molecule CD24 except for a single valine-alanine substitution due to a single-base polymorphism within the region of the gene coding for the extracellular domain. Biochemical analyses of the cloned cluster-w4 antigen confirmed both the presence of the phosphatidylinositol tail and the extensive glycosylation reported for the CD24 molecule. Furthermore, the cloned cluster-w4 antigen expressed on COS cells was shown to react with a comprehensive panel of CD24-specific monoclonal antibodies, as assessed by indirect immunofluorescence staining. Northern blot hybridization indicated the presence of several transcript sizes for the cluster-w4 antigen that were greatly overexpressed in small cell carcinoma cell lines, compared with normal hemopoietic cells and CD24-positive cell lines. Southern blot hybridization of restriction digests of genomic DNA identified a complex pattern of bands consistent with either a complex gene structure containing many exons or the presence of a family of closely related genes. Type Journal article Journal Cancer Res Publication Date 01/10/1992 Volume 52 Pages 5264 - 5270 Keywords Alanine, Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antigens, CD, Antigens, Neoplasm, Antigens, Surface, B-Lymphocytes, Base Sequence, CD24 Antigen, Carcinoma, Small Cell, Cloning, Molecular, DNA, Neoplasm, Humans, Lung Neoplasms, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Molecular Weight, Sequence Homology, Signal Transduction, Valine Freedom of Information Privacy Policy Copyright Statement Accessibility Statement Site Map Accessibility Cookies Contact us Log in About us About Us Our Impact Data Sharing Public Engagement Our History MRC WIMM Timeline - Accessible Version MRC WIMM Interactive Timeline Coronavirus Research Our COVID-19 research Coronavirus research in the MRC HIU Coronavirus research in the MRC MHU Life at the MRC WIMM Seminars Seminars Jordan Translational Medicine Lecture Seminars organized by the Women in Science Committee Weatherall Lectures Where to find us Where to find us Accessibility (Web) Accessibility A brief introduction to the MRC WIMM, from Professor KJ Patel Transcript: How planetary iron shaped life on Earth Diversity and Inclusion Diversity and Inclusion at the MRC WIMM Athena Swan MRC WIMM Diversity and Inclusion Committee Women in Science Leadership Programme Diversity in Science Seminar Series and Awards Ada Lovelace Day Staff Networks News Research Research Research Groups Blackford Group: DNA Damage and Disease Bodmer Group: Cancer and Immunogenetics Laboratory Fairfax Group: Genomics of Inflammation and Cancer Immunology McHugh Group: DNA Damage and Repair Goriely Group: Clinical genetics Wilkie Group: Clinical Genetics Ovarian Cancer Fulga Group: Genome Engineering and Synthetic Biology Sauka-Spengler Group: Gene Regulatory Networks in Development and Disease Higgs Group: Laboratory of Gene Regulation Chapman Group: Recombination mechanisms in immunity and cancer Patel Group: Two tier protection and metabolic genotoxicity during blood production Davies Group: Genomics and Clinical Genome Editing Developmental Immunology Research Group Davis Group: T-cell Biology Dong Group: Human T cell responses against Viruses & Cancer Drakesmith Group: Iron and Immunity Eggeling Group Ogg Group: Cutaneous Immunology Group Cornall Group: B cell development and immune regulation Jackson Group: Lymphatic Trafficking Research Group Bannard Group: B Cell Immunology Rehwinkel Group: Nucleic Acid Sensing Oxford Centre for Neuroinflammation Ho Group: Translational Lung Immunology Townsend Group: Molecular Immunology Gibbons Group: ATRX Group Fetal and Neonatal Haematology Group Porcher Group: Developmental Control of Blood Cell Fate Determination Milne Group: Epigenetic Control of Gene Expression in Leukaemia and Haematopoiesis Vyas Group: Biology and Treatment of Human Myeloid Cancers Nerlov Group: Hematopoietic Stem Cell Genetics Hughes Group: Genome Biology Jacobsen Group: Haematopoietic Stem Cell Biology Mead Group: Haematopoietic Stem Cell Biology Taylor Group: Analysis, Visualisation and Informatics Sims Group: Computational Genomics Childhood Leukaemia Research Group Chakraverty Group: Haematopoietic Transplantation and Immunotherapy Simmons Group: Intestinal Immunity in Health and Disease Psaila Group: Normal and Malignant Megakaryocyte and Platelet Biology de Bruijn Group: Developmental Haematopoiesis Sahakyan Group: Integrative Computational Biology and Machine Learning Wilkinson Group: Blood Stem Cell Fitness Mechanisms Koohy Group: Machine Learning and Integrative Approaches in Immunology Kusumbe Lab | Tissue and Tumor Microenvironments Facilities Wolfson Imaging Centre Flow Cytometry Facility Virus Screening Facility Mass Cytometry Facility Single Cell Facility Sequencing Facility Our Units and Centres MRC Human Immunology Unit MRC WIMM Centre for Computational Biology Our Units and Centres Oxford Single Cell Biology Consortium About Us Researchers Publications Platforms and Training News and Events Therapy Acceleration Laboratory (TAL) Therapy Acceleration Laboratory (TAL) Therapy Acceleration Laboratory (TAL) MRC Molecular Haematology Unit MRC Human Immunology Unit Centre for Computational Biology People Study With Us Study With Us Why join us How to Apply- 4 Year MRC WIMM Prize PhD Studentships Frequently Asked Questions Supervisors Antanaviciute Group - 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