The expected value of magnitude of winning and losing when playing a game Suppose we play a game at a casino. There is a \$5 stake and three possible outcomes: with probability $1/3$ you lose your stake, with with probability $1/3$ the bank returns your stake plus \$5, and with probability $1/3$ the bank simply returns your stake.
Let $X$ denote your winning in one play. And you play 1000 times. What would you expect the magnitude of your win or loss to be approximately?

Is this question asking the same as to find the $Var(S)$ where $S=X_1+\cdots X_{1000}$, so the final answer should be $1000\cdot Var(X)$?

If I understand correctly, you are interested in the expected value of the magnitude of the win (or) loss (and not the magnitude of the expected value of the win (or) loss). Hence, you are interested in computing $\mathbb{E}(S_n)$ of the underlying random variable where $$S_n = \left| X_1 + X_2 + \cdots + X_n \right|.$$
Note that $S_n$ can take values in the set $\{0,5,10,\ldots,5n\}$.
Now let us find out the probability that $S_n = 5m$ for some $m \in \{0,1,2,\ldots,n\}$.
This means we are interested in the event $X_1 + X_2 + \cdots + X_n = \pm 5m$.
Let us first evaluate the probability of the event $X_1 + X_2 + \cdots + X_n = 5m$.
For this event to occur, if you lose $k$ times, you need to win $m+k$ times and get back your stake the remaining $n-m-2k$ times where $k \in \left \{0,1,2,\ldots, \left \lfloor \frac{n-m}{2} \right \rfloor \right\}$.
Hence, the desired probability of the event $X_1 + X_2 + \cdots + X_n = 5m$ is given by $$\sum_{k=0}^{\left \lfloor \frac{n-m}{2} \right \rfloor} \frac{n!}{k!(m+k)!(n-m-2k)!} \frac1{3^n}$$
Hence, the desired probability of the event $S_n = 5m$ is given by $$P_n(m) = \sum_{k=0}^{\left \lfloor \frac{n-m}{2} \right \rfloor} \frac{n!}{k!(m+k)!(n-m-2k)!} \frac2{3^n}$$
Hence, the expected value of $S_n$ is given by $$\sum_{m=0}^{n} 5m P_n(m) = \sum_{m=0}^{n} \sum_{k=0}^{\left \lfloor \frac{n-m}{2} \right \rfloor} \frac{n!}{k!(m+k)!(n-m-2k)!} \frac{10m}{3^n}$$
