COMPOUND FOR ENHANCING THE COUPLING DEGREE OF COMPLEX TRPV4-KCA2.3 ^AND ANTI-HYPERTENSION APPLICATIONS THEREOF
FIG1 is a schematic diagram of the three-dimensional structures and functional zones of the protein TRPV4 and the protein KCa2 3
Experimental method: Possible binding sites (as shown in FIG1) are selected according to the three-dimensional structures and functional characteristics of the protein TRPV4 and the protein KCa2.3. The selected structural domains are mainly used to adjust the protein-protein relation, and are platforms where the proteins interact with each other. The selected binding sites are mutated such that the binding sites are lost. The used primers can be seen in Table 1.Table 1Gene namePrimer sequenceTRPV4ΔAR15'-gccaccccccatcctcaaaacggggaaga-3'5'-tcttccccgttttgaggatggggggtggc-3'TRPV4ΔAR25'-cgggagccgtcccgaggccagaca-3'5'-tgtctggcctcgggacggctcccg-3'TRPV4ΔAR35'-cagagacatctactactttggggagctgccct-3'5'-agggcagctccccaaagtagtagatgtctctg-3'TRPV4ΔAR45'-gggaggctacttctacagggggaacacggtg-3'5'-caccgtgttccccctgtagaagtagcctccc-3'TRPV4ΔAR55'-ggcgacaggactcggatggcctttcgcc-3'5'-ggcgaaaggccatccgagtcctgtcgcc-3'TRPV4ΔAR65'-acctggagacagttctcaacaatgatgaggacaccc-3'5'-gggtgtcctcatcattgttgagaactgtctccaggt-3'TRPV4ΔCaMBD5'-ctaccagtactatggcttcgagctgaacaagaactcaa-3'5'-ttgagttcttgttcagctcgaagccatagtactggtag-3'KCa2.3Δ17c5'-tggtgagctgagtgtcaaccacagctaccacaa-3'5'-catgtgcacaacttcatgatgctaaagaagattgaccatgcc-3'KCa2.3Δα15'-ggcatggtcaatcttctttagcatcatgaagttgtgcacatg-3'5'-gctccgtgattaagtcatacatgtcaatcttctttagcagcttt-3'Kca2.3Δloop5'-gtctataaacatacaaagctgcatgccaaagtcaggaaacac-3'5'-gtgtttcctgactttggcatgcagctttgtatgtttatagac-3'KCa2.3Δα25'-aaagctgctaaagaagattgacatgtatgacttaatcacggagc-3'5'-gctccgtgattaagtcatacatgtcaatcttctttagcagcttt-3'KCa2.3ΔCaMBD5'-ctccgtgattaagtcatacatcatcatgaagttgtgcacatg-3'5'-catgtgcacaacttcatgatgatgtatgacttaatcacggag-3'
schematic diagram
3
compound
13
diphenylacetyl chloride
['2. A preparation method of a compound for enhancing the space coupling degree of an endothelial cell ion channel complex TRPV4- KCa2.3 according to Claim 1,  characterized by  comprising the following steps: (1) providing propane diamine with t-butyloxycarboryl to protect a single amino first: (2) another exposed amino reacting with diphenylacetyl chloride to generate an amide: (3) removing a t-butyloxycarboryl protection from the amide under an acidic condition: (4) a compound obtained after removing the t-butyloxycarboryl protection performing an amino-ester exchange with 4-quinazolone-2-carboxylic acid ethyl ester to obtain a target compound:']