ELECTROPHORETIC SEPARATION DEVICES AND METHODS FOR USING THE SAME
FIG17 shows a graph of the effect of antibody fold-dilution on β-tubulin fluorescence readout signal for rat NSCs, according to embodiments of the present disclosure
Analytical performance of the scWestern blot - Antibody dilution factors of 1:20 were used to provide an acceptable balance between scWestern blot fluorescence signal and reagent consumption for EGFP and β-tubulin (FIG17). Under these probing conditions, consumption of 32 µg of each antibody was estimated per slide comprised of 6,720 scWestern blots or 4.8 ng per blot (compared to ∼0.5-2 µg of each primary antibody per lane of a conventional slab-gel western blot).
graph
17
Clause
30
were typically
18
filed May
100
including
400
from
2
±
20
with such as at
300
from
500
example
10
from
3
±
200
mm x from
['9. A method comprising: contacting a sample with a polymeric separation medium of any of the preceding claims; and applying an electric field to the polymeric separation medium in a manner sufficient to move at least some components of the sample from the microwell into the polymeric separation medium to produce separated sample components in the polymeric separation medium.']