CYCLIC NUCLEIC ACID MOLECULE HAVING GENE EXPRESSION CONTROL FUNCTION
FIG2 is an RP-HPLC chart of PA-0001-DSO
160 µmol/L PA-0001 (250 µL), 10 mmol/L DSO-ONSu/DMSO solution (160 µL), isopropanol (255 µL), 1% triethylamine aqueous solution (35 µL) were mixed and stirred at 40°C for 4 hr. The reaction mixture was purified by HPLC (column: Develosil C8-UG-5, 5 µm, 10×50 mm; flow rate: 4.7 mL/min; detection: UV260 nm; column oven: 40°C; Buffer A: 50 mmol/L TEAA (pH 7.0), 5% CH3CN; Buffer B: CH3CN) and the peak of the desired product was collected. The collected fraction was subjected to ethanol precipitation and the resulting precipitate was dissolved in distilled water for injection (Otsuka Pharmaceutical, Co., Ltd.). PA-0001-DSO with purity 97.39% was obtained. Mass spectrometry: 16364.20 (Calculated: 16364.35). The HPLC chart after purification is shown in FIG2.
flowchart
16
injection
19
about
2
preferably
1
Document is as Example
["1. A cyclic nucleic acid molecule represented by the following formula (A): wherein R 1 , R 2 , R 1'  and R 2'  are each independently a hydrogen atom, an alkyl group, an alkoxy group or hydroxy; R 3 , R 4 , R 3'  and R 4'  are each independently a hydrogen atom, an alkyl group, an alkoxy group or hydroxy; X 1  and X 2  are each independently an optionally modified ribonucleotide residue or deoxyribonucleotide residue; Y 1  and Y 2  are each independently an optionally modified ribonucleotide residue or deoxyribonucleotide residue; Z 1  - Z 4  are each independently an optionally modified ribonucleotide residue or deoxyribonucleotide residue; W 1  - W 6  are each independently an optionally modified ribonucleotide residue or deoxyribonucleotide residue; Q and T are each a guide strand or a passenger strand (when one is a guide strand, the other is a passenger strand); L is a linker; m and m' are each independently an integer of 1 - 12; n and n' are each independently an integer of 1 - 10; x1 and x2 are each independently 0 or 1; y1 and y2 are each independently 0 or 1; z1 - z4 are each independently 0, 1 or 2; and w1 - w6 are each independently 0, 1 or"]