 The long history of agricultural production , coupled with modern intensive agricultural methods such as pesticide and fertiliser use and runoff from contaminants into streams , rivers and lakes , impact the natural fresh @-@ water ecosystems and have placed pressure on biodiversity in Ireland . 
 The long history of agricultural production , coupled with modern intensive agricultural methods such as pesticide and fertiliser use and runoff from contaminants into streams , rivers and lakes , impact the natural fresh @-@ water ecosystems and <a href='been_done' title='1'><mark>have</mark></a> placed pressure on biodiversity in Ireland . 
The long history of agricultural production , coupled with modern intensive agricultural methods such as pesticide and fertiliser use and runoff from contaminants into streams , rivers and lakes , impact the natural fresh @-@ water ecosystems and done placed pressure on biodiversity in Ireland .