The development of the cerebellum involves a set of coordinated cell movements and two separate proliferation zones: the ventricular zone and the external granule cell layer (EGL), a rhombic-lip-derived progenitor pool . The EGL appears segregated during early cerebellum formation and produces only granule cells. Cerebellar granule cells (CGC) are the most abundant neurons of the brain . Their axons run as parallel fibres along the coronal axis, and the onedimensional spread of excitation that results from this arrangement is a key assumption in theories of cerebellar function. CGC receive inhibitory synaptic input from Golgi cells, which are mediated by gamma-aminobutyric acid (GABA). During both in vivo and in vitro development, CGC depend on the activity of the NMDA glutamate receptor subtype for survival and full differentiation . Cultured CGC are widely used as a model system for studying neuronal apoptosis.
It is recommended to use Neuronal Medium (NM, Cat. No. SC1521) for culturing MCGC in vitro.
Mouse Cerebellar Granule Cells are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
 Hatten ME. (1999) “Central nervous system neuronal migration.” Annu. Rev. Neurosci. 22, pp. 511-39.  Andersen BB, Korbo L, Pakkenberg B. (1992) “A quantitative study of the human cerebellum with unbiased stereological techniques.” J Comp Neurol. 326: 549-60.  Monti B, Marri L, Contestabile A. (2002) “NMDA receptor-dependent CREB activation in survival of cerebellar granule cells during in vivo and in vitro development.” Eur J Neurosci. 16: 1490-8.