sformed into new competent cells. Cells were grown on LB agar containing 20 μg/mL ampicillin to select for transformants that contained a recombined BPP plasmid, and 40 μg/mL IPTG to induce pLac-driven expression of mRFP. mRFP expression was detected under ultraviolet light and photographed using a BioRad imager.
Availability and requirements
Vector BBa_J64100 was generously provided by J. Tabor. KAH, WLH, JOD and EZ were supported by HHMI grant 52005120. SR was supported by a grant from iGEM at the Massachusetts Institute of Technology. ADB, MLB, TLB, ELJ KJM, BJO, JLP, LHH, and TTE were supported by the Missouri Western State University Summer Research Institute. LJH and AMC worked pro bono. KAH, AMC, LJH, and TTE are members of the Genome Consortium for Active Teaching . We thank the reviewers for their help with improving the final manuscript.
- Adleman LM: Molecular computation of solutions to combinatorial problems. Science 1994, 266: 1021-1024. 10.1126/science.7973651View Article
- Benenson Y, Paz-Elizur T, Adar R, Keinan E, Livneh Z, Shapiro E: Programmable and autonomous computing machine made of biomolecules. Nature 2001, 414: 430-434. 10.1038/35106533View Article
- Soreni M, Yogev S, Kossoy E, Shoham Y, Keinan E: Parallel biomolecular computation on surfaces with advanced finite automata. J Am Chem Soc 2005, 127: 3935-3943. 10.1021/ja047168vView Article
- Kossoy E, Lavid N, Soreni-Harari M, Shoham Y, Keinan E: A programmable biomolecular computing machine with bacterial phenotype output. Chembiochem 2007, 8: 1255-1260. 10.1002/cbic.200700180View Article
- Gates WH, Papadimitriou CH: Bounds for sorting by prefix reversal. Discrete Math 1979, 27: 47-57. 10.1016/0012-365X(79)90068-2View ArticleMathSciNet
- Bafna V, Pevzner P: Sorting by reversals: Genome rearrangements in plant organelles and evolutionary history of X chromosome. Molecular Biology and Evolution 1995, 12: 239-246.
- Hannenhalli S, Pevzner PA: Transforming Cabbage into Turnip: polynomial algorithm for sorting signed permutations by reversals. Journal of the ACM 1995, 46: 1-27. 10.1145/300515.300516View ArticleMathSciNet
- Bourque G, Pevzner PA, Tesler G: Reconstructing the genomic architecture of ancestral mammals: lessons from human, mouse, and rat genomes. Genome Res 2004, 14: 507-516. 10.1101/gr.1975204View Article
- Zieg J, Silverman M, Hilmen M, Simon M: Recombinational switch for gene expression. Science 1977, 196: 170-172. 10.1126/science.322276View Article
- Zieg J, Simon M: Analysis of the nucleotide sequence of an invertible controlling element. Proc Natl Acad Sci USA 1980, 77: 4196-4200. 10.1073/pnas.77.7.4196View Article
- Johnson RC, Bruist MF: Intermediates in Hin-mediated DNA inversion: a role for Fis and the recombinational enhancer in the strand exchange reaction. EMBO J 1989, 8: 1581-1590.
- Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S: New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl Environ Microbiol 1998, 64: 2240-2246.
- Lim HM, Hughes KT, Simon MI: The effects of symmetrical recombination site hixC on Hin recombinase function. J Biol Chem 1992, 267: 11183-11190.
- Moskowitz IP, Heichman KA, Johnson RC: Alignment of recombination sites in Hin-mediated site-specific DNA recombination. Genes Dev 1991, 5: 1635-1645. 10.1101/gad.5.9.1635View Article
- Johnson RC, Bruist MB, Glaccum MB, Simon MI: In vitro analysis of Hin-mediated site-specific recombination. Cold Spring Harb Symp Quant Biol 1984, 49: 751-760.View Article
- Lim HM, Simon MI: The role of negative supercoiling in Hin-mediated site-specific recombination. J Biol Chem 1992, 267: 11176-11182.
- Merickel SK, Johnson RC: Topological analysis of Hin-catalysed DNA recombination in vivo and in vitro. Mol Microbiol 2004, 51: 1143-1154. 10.1046/j.1365-2958.2003.03890.xView Article
- Glascock CB, Weickert MJ: Using chromosomal lacIQ1 to control expression of genes on high-copy-number plasmids in Escherichia coli. Gene 1998, 223: 221-231. 10.1016/S0378-1119(98)00240-6View Article
- Eckert B, Beck C