 on average, 8.86% when considering coefficients of variance for triplicate injections of all peptides at all dilutions.
ANOVA analysis of allergen levels.
|Beta conglycinin alpha||Site||5||15||15.38||<0.0001|
|Beta conglycinin alpha||Cultivar||3||15||0.92||0.4558|
|Gly m Bd 28k||Site||5||15||19.07||<0.0001|
|Gly m Bd 28k||Cultivar||3||15||4.47||0.0197|
For a given variable, genotypic variation, and environmental variation were quantified relative to residual variation using Analysis of Variance (ANOVA)-based F-test.
Allergen peptide analyte information.
|ID no.||Peptide name||Protein represented||Peptide sequence||Calculated (Da)||Observed (Th)||Glyma no.||Protein MW (g/mol)monoisotopic (M+H)1+|
|Gly m 6 (GLYCININ)|
|SA2||GlyG1-2||G1 subunit (Bx)||VLIVPQNFVVAAR||1425.857604||713.4||Glyma03g32030.1||55671.6|
|SA4||GlyG2-2||G2 subunit (A2)||NLQGENEEEDSGAIVTVK||1931.919212||966.5||Glyma03g32020.1, Glyma03g32020.2||54356.887636, 41045.114403|
|SA6||GlyG3-2||G3 subunit (A)||FYLAGNQEQEFLQYQPQK||2231.076708||1116.0||Glyma19g34780.1||54207.863911|
|SA7||GlyG4||G4 subunit (A5)||VESEGGLIQTWNSQHPELK||2152.066872||718.0||Glyma10g04280.1||63758.458975|
|Gly m 5 (BETA CONGLYCININ)|
|SA10||Bcon α||Alpha subunit||LITLAIPVNKPGR||1391.873253||464.6||Glyma20g28650.1, Glyma20g28650.2, Glyma20g28660.1||70263.437646, 63248.763394, 70263.437646|
|Gly mTI (KUNITZ TRYPSIN INHIBITOR)|
|SA11||KTI 3-1||KTI 3||FIAEGHPLSLK||1211.678251||404.6||Glyma08g45530.1||14517.404983|
|SA13||KTI 1||KTI 1||DTVDGWFNIER||1351.627676||676.3||Glyma01g10900.1||22432.404593|
|Gly m BD (P34)|
Soy peptide data – Peptide sequences, masses, and observed masses for all peptides used in absolute quantification experiments are presented. In addition, the name of the annotated Swiss-Prot entry (“Protein represented” column) that corresponds to the soybean genome sequence (“Glyma no.”, Schmutz et al., 2010; www.phytozome.org) used to design the peptide, is presented. In the case where multiple splice variants were present, the underlined information was used. The uncharged monoisotopic mass of the protein sequences represented by the Glyma numbers which were used in molar quantity calculations, are presented.
Allergen peptide MRM information.
|Precursor (Th)||Precursor charge||Product (Th)||Product charge||Ion type|
Soy allergen MRM – Peptide sequence, precursor, and product masses and charge states along with product ion types for the multiplexed MRM allergen assay, are presented.
Keywords: soybean, GMO, allergens, MRM, LC-MS/MS, glycinin.
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