 long oval-shaped cells. Looked similar to paramecium.
Conclusions The first part of this lab helped to characterize whether the bacterial growth on the tetracycline plates were antibiotic-resistant or not. Observing the cells more closely on a wet mount allowed for further analysis of the type of cell. Gram-positive and negative analysis were done on the samples from both a nutrient agar plate and from a tetracycline plate. The bacteria found on the nutrient agar plate were found to be gram negative, showing a pink color to the stain. The bacteria found on another sample of nutrient agar was found to be gram-positive, however. The bacteria found on the tetracycline plate were found to be gram-negative, and looked similar to sperillium cells. The final portion of this lab saw the beginning of DNA sequencing of the bacteria found on the plates through PCR.
Objectives 1. To understand the characteristics and diversity of plants 2. To appreciate the function and importance of fungi
The first objective will be addressed by collecting plant samples from our transects and observing/characterizing them into a major plant group. Vascularization, types of leaves, and seeds were described in order to properly characterize the type of plant. The second objective will be addressed through observing the fungi from agar plates that were preserved from the microbiology lab.
Collecting Plant Samples 1. Fill one bag with leaf litter from transect 2. Collect five different plant samples (from already dead plants)
Plant Vascularization 1. Observe the cross-section of a moss and lily slide 2. Note the vascularization of those plants 3. Note the vascularization of the plants collected from transect
Plant Specialization 1. Examine leaves of moss 2. Observe and describe the shape, size, and cluster arrangement of the leaves collected from transect
Plant Reproduction 1. Study the bryophyte reproductive cycle and identify male and female gametophytes and sporophyte 2. Dissect a lily flower sporophyte and identify the parts 3. Dissect any seeds collected at transect and identify the parts, identify as either monocot or dicot
Observing Fungi 1. Observe fungi on agar plates from microbiology lab
Berlese Funnel Set-Up 1. Pour 25 ml 50:50 ethanol/water solution into a flask 2. Screening material placed at the bottom of the funnel 3. Funnel is placed in neck of flask 4. Leaf litter goes inside the funnel 5. Funnel/flask is placed underneath a light fixture
The five leaves that we collected were identified as two types of clover leaves, a smooth toothed heart-shaped leaf, a sage leaf, and a possible western cedar leaf. Our leaves showed that the plants were vascular. Other details were unable to be determined by the leaves from the transect as there was not much else growth to collect and observe.
This lab helped us learn how to characterize plants based on certain characteristics such as shape, size and vascularization. Although we were unable to find many leaves in our transect, the leaves we did identify showed us that much of our transect was not natural as most of the plants had been planted. This lab also taught us a new method in identifying invertebrates in our transects. The Berlese Funnel will hopefully show the various invertebrates that live in the transect.
Objectives 1. To understand the importance of invertebrates 2. To learn how simple systems (including specialized cells and overall body plan) evolved into more complex systems
The first objective will be addressed through observing acoelomates, pseudoacoelomates and coelomates and how they move. The second objective will be addressed through analyzing the invertebrates collected from the Berlese Funnel that was set up in the previous week.
Observing Acoelomates, Pseudocoelomates, and Coelomates 1. Observe Acoelomates and its cross-section 2. Observe nematodes and pseudocoelomates 3. Observe Annelida, the coelomate
Analyzing Invertebrates 1. Contents of Berlese Funnel are transferred to a petri dish 2. Observe contents under the dissecting microscope and identify any invertebrates
The c