een means' shows just that: The difference between the mean of group A and the mean of group B. Prism calls that value "Std. When To Use Standard Deviation Vs Standard Error
Got a question you need answered quickly? The midpoint that Prism computes is the logEC50, which will have a distribution closer to a Gaussian distribution than will the EC50. CLICK HERE > On-site training LEARN MORE > ©2016 GraphPad Software, Inc. this page One suggestion: Convert all your values to base-10 logs.
Here is a more conventional approach. Standard Deviation Error Bars If you go down one SE, you are at -7 (0.1 uM). The 95% confidence interval for the mean of the logEC50 values ranges from -7.649 to -7.439.
URL of this page: http://www.graphpad.com/support?stat_standard_deviation_and_standar.htm © 1995-2016 GraphPad Software, Inc. That's not true for the geometric mean, which is the antilog of the mean of the logs. All rights reserved. Standard Deviation Divided By Mean The SD tells you about the variability or scatter of the values.
Ifyou have a XY or Grouped table:Choose "Row means/totals". The SE of logEC50 can be used to compute a confidence interval, and the two confidence limits can then be transformed to antilogs. All rights reserved. http://askmetips.com/standard-deviation/standard-error-mean-graphpad-prism.php That is used to compute the confidence interval for the difference between the two means, shown just below.
What about quantifying the scatter? All rights reserved. All rights reserved. If you compute the antilog of 1, which is 10, you certainly cannot say that the SE of the EC50 is 10 Molar!
The 95% confidence interval contains zero (the null hypothesis, no difference between means), which is consistent with a P value greater than 0.05. Some people prefer to report SE values than confidence intervals, so Prism reports both. This range is centered on the mean logEC50 (-7.544). If, as is typical, your doses are equally spaced on a LOG scale, then the uncertainty of the IC50 (or EC50) is symmetrical on a LOG scale.
All rights reserved. Error" or SE, the most conventional label.