Lecture 1: DNA as genetic material
Lecture 2: Structure of DNA and RNA
Lecture 3: Bacterial Restriction/Modification System
Lecture 4: DNA Modifying Enzymes
Lecture 5: Intro to Prokaryotic DNA Replication
Lecture 6: Prokaryotic DNA Replication, cont.
Lecture 7: DNA Supercoiling
Lecture 8: DNA Supercoiling cont., Topoisomerases
Lecture 9: Introduction to Bacteria
Lecture 10: Introduction to Bacteria, cont.
Lecture 11: Bacterial Reproduction and Growth
Lecture 12: Bacterial Growth, cont.
Lecture 13: Extrachromosomal Elements, Plasmids, Selectable Markers
Lecture 14: Central Dogma; Genetic Code
Lecture 15: Gene and Operon
Lecture 16: The lac Operon, CAP Site
Lecture 17: lac Operon, cont., DNA Footprinting
Lecture 18: Transcriptional Regulation: Transcription Termination, the trp Operon
Lecture 19: mRNA Translation
Lecture 20: Gel Electrophoresis
Lecture 21: DNA Sequence Analysis
Lecture 22: Polymerase Chain Reaction (PCR)
Lecture 23: PCR, cont.
Lecture 24: Cloning PCR Products
Lecture 25: Prokaryotic Expression Vectors
Lecture 26: Protein Sequencing, Peptide Mapping, Synthetic Genes
Lecture 27: cDNA Libraries
Lecture 28: Genomic Libraries
Lecture 29: Protein Purification: Assays, Initial Steps, Resins
Lecture 30: Protein Purification: Ion exchange elutions, dialysis, concentration
Lecture 31: Protein Purification: Types of Resins, cont. Plumbing
Lecture 32: Protein Purification, Running the Experiment, Resolving Peaks
Lecture 33: Bacteriophage M13, M13mp Vectors
Lecture 34: M13 Phage Display, bacterial display, DNA binding protein display Libraries
Lecture 35: SELEX System
Lecture 36: Protein-Protein Recognition Probed Using a Yeast Transcriptional Activator System
Lecture 37: Molecular Imprinting
Lecture 38: T.B.A.
1998 Dr. Michael Blaber