cacodylate buffer-NaOH, pH 7.4, for 2.5 h at 4°C and rinsed three times (20 min per rinse) with 130 mM sucrose, 10 mM 2-mercaptoethanol in 100 mM sodium cacodylate buffer-NaOH, pH 7.4. They were then post-fixed with 1% (w/v) osmium tetroxide in 100 mM sodium cacodylate buffer-NaOH, pH 7.4, rinsed three times (5 min per rinse) with ultrapure (Milli-Q) water and dehydrated through a graded series of acetone (20%, 50%, 70%, 90%, 100% v/v). After infiltration through a graded acetone/Epon/Spurr’s epoxy resin series, samples were embedded in 100% w/v Spurr’s epoxy resin and polymerized at 60°C for 24 h. Ultrathin sections were prepared using a Diatome diamond knife on an 8800 Ultratome III (LKB Instruments) and stained with uranyl acetate and lead citrate. The stained sections (about 20 per treatment) were examined under a JEM-1200EX transmission electron microscope (JEOL), and images were recorded on 4489 film (Eastman-Kodak).
RNA-seq: RNA-sequencing; MGDG: Monogalactosyldiacylglycerol; TCA: Tricarboxylic cycle; TAG: Triacylglycerol; PUFA: Polyunsaturated fatty acid; SFA: Saturated fatty acid; KEGG: Kyoto encyclopedia of genes and genomes; LHC: Light harvesting complex; PSII: Photosystem II; ROS: Reactive oxygen species; PEPC: Phosphoenolpyruvate carboxylase; OAA: Oxaloacetate; CCM: Carbon concentrating mechanism; GS: Glutamine synthetase; PEP: Phosphoenolpyruvate; ME: Malic enzyme; ACCase: Adetyl-CoA carboxylase; FAS: Fatty acid synthase; FTIR: Fourier transform infrared spectrometer; FBP: Fructose-1,6-bisphosphatase; DGAT: Diacylglycerol acyltransferase; PDAT: Phospholipid:diacylglycerol acyltransferase; PC: Phosphatidylcholine; RPKM: Reads per kilobase per million mapped reads; FA: Fatty acid; DW: Dry weight.
The authors declare that there are no competing interests.
HL and YG designed the research; ZY, YN, YM, JX, MZ, WY, JL, YG and HL performed research; YG, ZY and HL contributed analytic and computational tools; ZY, YN, WY, JL, SL, YG and HL analyzed data; ZY, YG and HL wrote the paper. All authors read and approved the final manuscript.
This work was supported by the National Technologies Program (2011BAD14B03), Science and Technology Project of Guangdong (2010B030600005, 2009B020301002, 2009B050600005), and the Fundamental Research Funds for the Central Universities. Y.G. is supported by NIH 1R21NS082212-01 and EU-FP VII Systems Biology of Rare Disease.
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