 several contiguous probes.
(8.2 MB PDF)
Figure S11. Plots of Neighbor Dissimilarity (log2) against IP:WCE Hybridization Ratios for Each Probe of Chromosome I using Data from Both Haploid and Diploid Experiments (Strains GP6013 and GP6203)
(A) 0 h, haploid.
(B) Mean 5 h, haploid.
(C) 0 h, diploid.
(D) 5 h, diploid.
Note that, in comparison to Figure S10, spikes have largely been removed but genuine Rec12-enrichment peaks remain.
(7.8 MB PDF)
Table S1. rec12-FLAG and rec12-HA Are Recombination Proficient
(26 KB DOC)
Table S2. Correlation of DSB Hotspots and GO Classes
(31 KB DOC)
Table S3. S. pombe Strains
(41 KB DOC)
Table S4. Primers for Plasmid and Allele Construction and PCR Analysis
(34 KB DOC)
Text S1. Construction of pJF32 (rec12-201::6His-2FLAG) and pJF41 (rec12-202::6His-2HA)
(23 KB DOC)
We are grateful to Yukang Lin for rec12 plasmids; Michael Lichten, Kim Lindstrom, Toshi Tsukiyama, and Iestyn Whitehouse for advice on immunoprecipations and their analysis by PCR; Chad Ellermeier for unpublished data; Jeff Delrow, Katherine Guthrie, and Harlan Robins for help with data analysis; and Jürg Kohli and Katja Ludin for exchange of data before publication. We thank Sue Amundsen, Luther Davis, and Walt Steiner for helpful comments on the manuscript.
GAC, RWH, SISG, and GRS conceived the experiments. RWH, HPC, and JAF performed the experiments. GAC and GRS analyzed the data and wrote the paper.
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