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[Figure ID: F1]
Experimental design and processing workflows. (A) Each cultivar (92B12, 92M10, 92M72, and 93B15) was grown in each location (NE, IA, PA, ONT, KS, and GA) in triplicate using a randomized complete block design for the plots. Different states/provinces in the map are colored to distinguish the three major climate zones represented within the chosen growth regions. Stars within each state/province indicate the location where the soybean cultivation occurred. (B) Three hundred grams of soybean was harvested from each block (each replicate). The replicate material was pooled and ground, with 10 seeds worth of meal portioned for analysis. Protein was extracted in quadruplicate from each, and analyzed using multiple reaction monitoring with AQUA™ peptide internal standards. Absolute quantitative values for soybean allergens and anti-nutritional factors were calculated and compared for variance between cultivars and environments (locations).
[Figure ID: F2]
Environmental and varietal variation. Percent coefficients of variation (%CV, standard deviation(−mean × 100) for allergen quantities are plotted for each allergen with respect to environment and cultivar. The variation was normalized with respect to the mean. This variation was also analyzed using ANOVA, presented in Table