M Medical School.
"Now that we are using microfluidics for fertilization, what you are starting to see is the whole IVF process happening on a chip," says Smith, senior author of a study in Human Reproduction and director of the Assisted Reproductive Technologies Laboratory and of the Gamete Cryopreservation Laboratory at the Comprehensive Cancer Center.
IVF is a process in which eggs are removed from a woman's body and fertilized with sperm outside the body. Fertilized eggs are then placed in the woman's uterus, where they can develop as in a normal pregnancy.
The study, published online in the journal Human Reproduction, suggests that among other uses, microfluidic channels could be used in some – but not all – instances when a common form of insemination, known as ICSI, otherwise would be employed. ICSI, which stands for intracytoplasmic sperm injection, involves a single sperm being injected directly into an egg, or oocyte.
Smith says ICSI still will be used in many situations, particularly when other types of fertilization have failed in the past, or when the man has an extremely low sperm count or motility. Smith does not think the use of microfluidics will replace ICSI, but he says it could offer another option to many couples whose situations do not require ICSI, a process that can cost an extra $1,500 to $2,500 in addition to standard IVF costs.
"While ICSI bypasses all natural selection, the use of microfluidic channels more closely resembles in vivo insemination. The microfluidic environment also may possess conditions more suitable for efficient sperm-oocyte interaction than the culture dish," he says.
During the early stages of the study, researchers found that, contrary to their initial hypothesis, a much lower fertilization rate was achieved with the microfluidic device (12 percent) than in culture dishes (43 percent). They then hypothesized that as sperm concentration is decreased, fertilization rates would improve in microchannels. At these lower concentrations, the combined fertilization rate wa