ion against disease. Iran also is very famous in producing essential oil and water extraction from national plants spatially Rosa damacena and menthe spp. All essential oil Samples were analysed by GC and GC/MS. The main constituents of oil extracted traditionally, samples were extracted by two hydro distillation method which designed by authors in Research Institute of Forests and Rangelands. were analayz and find it citronellol, cis-p-menth -2-en-1-ol and geraniol in Rosa damecena. These compounds and others are very popular in producing drug and effects as anti-cancer, anti-bacterial out of that and we should take care of over doses or toxicology. Iran, on herbal played a key role in connecting various cultures and civilizations. Ethno-herbal and phyto- chemical dates back to a long time ago and a number of writings regarding this issue are left by great physicians e.g. Avicenna and Rhazes. The focus of this paper is on the analytical methodologies, which include the extraction, isolation and characterization of active ingredients in herbal preparations.