I have a question regarding the RNA-Seq analysis.We have a time course data regarding a mouse model wt and mutant treated with a drug (10uM) and the taken down at different time points: 8 time points and did RNA-seq on these samples currently we have tpm values and raw gene counts from rna seq. Also we have only one sample for each time point. we would like to see for changes in genes across different time point but not sure statistical method should we use to determine this, should we consider for example a single time point wt vs mt, or see trends across wt and mt individually I know that there are some bioc packages for NGS analysis (edgeR,DESeq,etc). Is any package or method good for one-sample-per-time-pointdata?
ngs time course experiment
rna seq;software recommendation;statistics