For identification of macromolecular complexes in cryoelectron tomo-   grams covering cytoplasmic regions, we used a combination of  subtomogram  averaging and classification. In a first step, the cross  correlation function  between two times binned tomograms and a 25 nm  spherical template  was computed. From the corresponding correlation  peaks subvolumes  (24  24  24 voxels) were extracted and classified  into 12 classes via a missing-wedge corrected multi reference  classification procedure, using the    initial template with different  representations of Gaussian noise added as    starting references.
What does missing-wedge corrected mean?
image processing