NI data acquisition cards offer a possibility of synchronising data acquisition with the pulses of a 80 MHz Ti:Sapphire laser. This supposedly helps improve the signal to noise ratio, since the true fluorescence signals should be time-locked to the laser pulses, while electrical noise in the PMT amplifier circuits and noise from the PMT itself are not.Has anyone done a head-to-head comparison of SNR on a two-photon microscope with and without triggered acquisition?
Is there a tangible benefit of synchronising the sample clock to the laser pulses in a resonant scanning two-photon microscope?
neurobiology;neuroimaging