Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3′ end of pyrimidine residues, forming oligoribonucleotides having 3′-terminal pyrimidine-3′-phosphates. Pyrimidine-3′-monophosphates are also released by RNase A cleavage of adjacent pyrimidine nucleotides. Modified RNA containing pyrimidine-2′-fluoro-dNMPs, such as DuraScript® RNA made by in vitro transcription using the DuraScribe® T7 & SP6 Transcription Kits is completely resistant to cleavage by RNase A.
Removal of RNA from DNA preparations. Removal of unhybridized regions of RNA from DNA-RNA or RNA-RNA hybrids.
50% glycerol containing 25 mM sodium acetate (pH 4.6).