Little quantitative evidence exists regarding how effective protected areas are for preserving species. We compared dung beetle assemblages (Coleoptera : Scarabaeidae : Scarabaeinae) inside and outside of the Kruger National Park, which protects indeigenous flora and fauna over a large area of savannah in the northeast lowlands of South Africa. Although it is contiguous with other reserves in South Africa, Zimbabwe and Mozambique, parts of its border abut onto farmland. Some effects of differing land usage either side of this border were studied at the South African Wildlife College (24.541° S 31.335° E) and the nearby farming village of Welverdiend using dung beetle assemblage structure (Coleoptera: Scarabaeidae: Scarabaeinae) as indicators. Samples were taken from gabbro-derived and granite-derived soils in open woody vegetation, both within the reserve and on adjoining farmland, using composite pig, elephant and cattle dung baits in the early rainy season (November 2009) and separate pig and elephant dung baits in the late rainy season (March 2010). Despite much higher large mammal density around Welverdiend, significantly greater species richness, abundance, and biomass of dung beetles were recorded in the reserve where mammal species diversity is greater and elephants produce much larger droppings than any mammal in the farmland. Assemblage structure also differed strongly between dung types, weather conditions on sample days, and season, but weakly between sampled soil types. These differences in assemblage structure were recorded over short distances as the sites in the reserve were only 3-4 km from those in farmland at Welverdiend.