Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm (, ). In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs ( ). tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs . Does not show tRNA pseudouridylate synthase activity in the nucleus . In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity . Binds pri-miRNAs . Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage (, ). Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death .
Subcellular locations: Nucleus, Cytoplasm, Mitochondrion
Localizes mainly in the nucleus (Probable) . tRNA pseudouridylate synthase activity is restricted to the cytoplasm . Translocates from nucleus to mitochondria during TRAIL-induced apoptosis .