A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, potentially modulating endocannabinoid signaling. Has no hydroxylase activity toward various fatty acids, steroids and prostaglandins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
Subcellular locations: Endoplasmic reticulum membrane, Microsome membrane
Expressed in brain, heart, kidney and skin and, at lower levels, in skeletal muscle and liver (, ). In the brain, high levels are detected in amygdala and lower levels in globus pallidus and cerebellum . In the heart, very high levels in aorta, but very low levels in other heart regions (, ). Also expressed in breast, prostate and colon .