Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis ( , ). Required for efficient progress through mitosis and cytokinesis . Required for normal formation of the cleavage furrow at the end of mitosis . Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis . Plays a role in macropinocytosis . Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation . Stimulates the GTPase activity of DNM2 . Promotes DNM2 location at the plasma membrane . Together with DNM2, involved in autophagosome assembly by regulating trafficking from recycling endosomes of phospholipid scramblase ATG9A .
Subcellular locations: Endomembrane system, Endosome membrane, Recycling endosome membrane, Cell membrane, Cytoplasmic vesicle membrane
Localized at sites of endocytosis at the cell membrane . Detected on newly formed macropinosomes . Partially colocalized with clathrin and dynamin at the cell membrane . Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation .