Pseudouridylate synthase that catalyzes pseudouridylation of tRNAs and mRNAs (, ). Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA . Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (, ). Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions . Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing . Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 mRNA .
Subcellular locations: Mitochondrion
Subcellular locations: Nucleus, Cytoplasm
Widely expressed . High levels of expression found in brain and skeletal muscle .