E3 ubiquitin-protein ligase involved in DNA damage response by promoting DNA resection and homologous recombination (, ). Recruited to sites of double-strand breaks following DNA damage and specifically promotes double-strand break repair via homologous recombination (, ). Two different, non-exclusive, mechanisms have been proposed. According to a report, regulates the choice of double-strand break repair by favoring homologous recombination over non-homologous end joining (NHEJ): acts by mediating ubiquitination of XRCC5/Ku80, leading to remove the Ku complex from DNA breaks, thereby promoting homologous recombination . According to another report, cooperates with UBE2Ds E2 ubiquitin ligases (UBE2D1, UBE2D2, UBE2D3 or UBE2D4) to promote homologous recombination by mediating ubiquitination of RBBP8/CtIP . Together with NLK, involved in the ubiquitination and degradation of TCF/LEF . Also exhibits auto-ubiquitination activity in combination with UBE2K . May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway .
Subcellular locations: Chromosome
Recruited at DNA damage sites . Localizes to sites of double-strand break: localization to double-strand break sites is mediated by the zinc fingers (, ).