Component of the MMS22L-TONSL complex, a complex that promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks (   ). The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication ( , ). It mediates the assembly of RAD51 filaments on single-stranded DNA (ssDNA): the MMS22L-TONSL complex is recruited to DSBs following histone replacement by histone chaperones and eviction of the replication protein A complex (RPA/RP-A) from DSBs ( , ). Following recruitment to DSBs, the TONSL-MMS22L complex promotes recruitment of RAD51 filaments and subsequent homologous recombination (, ). Within the complex, MMS22L acts by binding ssDNA .
Subcellular locations: Nucleus, Chromosome
Localizes to DNA damage sites, accumulates at stressed replication forks ( ). Recruited to stalled or collapsed replication forks; directly binds replication protein A complex (RPA/RP-A)-coated single-stranded DNA (ssDNA) .