In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production . In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration . Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro . However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo . Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (, ). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis .
Subcellular locations: Cell membrane, Membrane raft, Secreted, Cytoplasmic granule membrane, Cell projection, Lamellipodium
Cell surface expression on neutrophils is increased upon TNF-alpha, fMLP or CXCL8/IL8-mediated stimulation (, ). In neutrophils, stored predominantly in secondary and tertiary granules . Can also be shedded from the cell membrane (, ). Localizes to lamellar protrusions in spreading neutrophils .
Highly expressed in normal bone marrow and weakly expressed in fetal liver . During neutrophil differentiation, expression begins at the metamyelocyte stage and continues throughout the subsequent stages (at protein level) ( ). Expressed by a subset of mature neutrophils (at protein level) (     ). The percentage of neutrophils expressing CD177 varies across the population (, ). Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET) (, ).