NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery (  ). Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of CIAPIN1, another key component of the CIA machinery ( ). In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins (, ). It can also reduce the [2Fe-2S] cluster of CISD1 and activate this protein implicated in Fe/S cluster repair . In vitro can fully activate methionine synthase/MTR in the presence of soluble cytochrome b5/CYB5A .
Subcellular locations: Cytoplasm, Perinuclear region
Concentrated in perinuclear structure.
Low expression in brain, heart, kidney, pancreas, prostate and skeletal muscle. Highest levels in the placenta. Expressed in cancer cell lines including promyelocytic leukemia, HeLaS3, chronic myelagenous leukemia, lymphoblastic leukemia, Burkitt's lymphoma, colorectal adenocarcinoma, lung carcinoma, and melanoma G-361.