Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.
Subcellular locations: Cytoplasm, Mitochondrion, Cell projection, Axon, Secreted, Secreted, Extracellular exosome
Secreted by motor neuron, possibly through the exosome pathway (By similarity).