Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. Required to maintain the integrity of the Golgi apparatus (, ). Required for microtubule nucleation at the cis-side of the Golgi apparatus (, ). Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase . In complex with PDE4DIP isoform 13/MMG8/SMYLE, recruits CAMSAP2 to the Golgi apparatus and tethers non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (, ). In complex with PDE4DIP isoform 13/MMG8/SMYLE, EB1/MAPRE1 and CDK5RAP2, contributes to microtubules nucleation and extension also from the centrosome to the cell periphery .
Associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations.
Subcellular locations: Golgi apparatus, Cytoplasm, Cytoplasm, Cytoskeleton, Microtubule organizing center, Centrosome
Cytoplasmic in parietal cells . Recruited to the Golgi apparatus by GM130/GOLGA2 . Localization at the centrosome versus Golgi apparatus may be cell line-dependent. In SKBr3 and HEK293F cells, exclusively located at the centrosome . In HeLa, MDA-MB231 and RPE-1 cells, detected at the Golgi apparatus (, ). In SK-BR-3 cells, recruited to the centrosome in the presence of CDK5RAP2 .
Widely expressed . Isoform 4: Highly expressed in skeletal muscle and in pancreas .