Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation ( ). Preferentially uses palmitoleate, oleate and linoleate . Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).
Subcellular locations: Mitochondrion outer membrane, Peroxisome membrane, Microsome membrane, Endoplasmic reticulum membrane
Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.