Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate ( ). Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling .
Sequesters MAD2L1 in the cytoplasm preventing its function as an activator of the mitotic spindle assembly checkpoint (SAC) resulting in SAC impairment and chromosomal instability in hepatocellular carcinomas.
Subcellular locations: Nucleus, Chromosome, Centromere, Kinetochore, Nucleus envelope, Cytoplasm, Cytoskeleton, Microtubule organizing center, Centrosome, Cytoplasm, Cytoskeleton, Spindle, Cytoplasm, Cytoskeleton, Spindle pole
Co-localizes with TPR at the nucleus envelope during interphase and throughout the cell cycle (, ). From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody . Localizes to kinetochores during prometaphase (, ). Does not localize to kinetochores during metaphase . Colocalizes with NEK2 at the kinetochore . Colocalizes with IK at spindle poles during metaphase and anaphase .
Subcellular locations: Cytoplasm
Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level).
Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level).