Metallocarboxypeptidase that mediates protein deglutamylation of tubulin and non-tubulin target proteins ( ). Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of alpha- and beta-tubulin ( ). Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate . Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of alpha-tubulin as well as non-tubulin proteins such as MYLK . Involved in KLF4 deglutamylation which promotes KLF4 proteasome-mediated degradation, thereby negatively regulating cell pluripotency maintenance and embryogenesis .
Subcellular locations: Cytoplasm, Cytoplasm, Cytosol, Nucleus, Mitochondrion
Localizes in both the cytoplasm and nuclei of interphase and dividing cells.