Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism . Converts citramalyl-CoA into acetyl-CoA and pyruvate in the C5-dicarboxylate catabolism pathway . The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite . Also acts as a malate synthase in vitro, converting glyoxylate and acetyl-CoA to malate (, ). Also displays malyl-CoA thioesterase activity . Also acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate (, ). Also has very weak citramalate synthase activity in vitro (, ).
Subcellular locations: Mitochondrion