Catalytically inactive when either DNA or nucleosomes are the substrate and does not possess chromatin-remodeling activity (, ). Acts as a negative regulator of chromatin remodelers by generating inactive complexes .
Helicase that possesses intrinsic ATP-dependent chromatin-remodeling activity ( , ). ATPase activity is substrate-dependent, and is increased when nucleosomes are the substrate, but is also catalytically active when DNA alone is the substrate ( ). Catalytic subunit of ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair ( , ). Within the ISWI chromatin-remodeling complexes, slides edge- and center-positioned histone octamers away from their original location on the DNA template . Catalytic activity and histone octamer sliding propensity is regulated and determined by components of the ISWI chromatin-remodeling complexes . The BAZ1A-, BAZ1B-, BAZ2A- and BAZ2B-containing ISWI chromatin-remodeling complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template . The CECR2- and RSF1-containing ISWI chromatin-remodeling complexes do not have the ability to slide mononucleosomes to the center of a DNA template . Within the NURF-1 and CERF-1 ISWI chromatin remodeling complexes, nucleosomes are the preferred substrate for its ATPase activity (, ). Within the NURF-1 ISWI chromatin-remodeling complex, binds to the promoters of En1 and En2 to positively regulate their expression and promote brain development . May promote neurite outgrowth . May be involved in the development of luteal cells .
Subcellular locations: Nucleus
Mainly expressed in non-neuronal tissues such as lung, breast, kidney, and ovary.
Expressed in lung, breast, kidney, ovary, skeletal muscle and brain.