Cytoplasmic and mitochondrial threonylcarbamoyl-AMP synthase required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine ( ). Catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate . Participates in t(6)A37 formation in cytoplasmic and mitochondrial tRNAs . May regulate the activity of some transporters (By similarity).
Subcellular locations: Cytoplasm, Mitochondrion, Cell membrane
A large fraction localizes in the cytoplasm, whereas a smaller fraction is imported to mitochondria.
Ubiquitously expressed.