Together with methyltransferase FTSJ1, methylates the 2'-O-ribose of nucleotides at position 34 of the tRNA anticodon loop of substrate tRNAs (, ). Required for the correct positioning of the substrate tRNA for methylation . Required to suppress amino acid starvation-induced autophagy . Enhances the STK11/LKB1-induced cell growth suppression activity .
Subcellular locations: Cytoplasm
Colocalizes in the cytoplasm with STK11/LKB1.
Ubiquitous.