Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation. Responsible for the sulfation of cholesterol (, ). Catalyzes sulfation of the 3beta-hydroxyl groups of steroids, such as, pregnenolone and dehydroepiandrosterone (DHEA) ( , ). Preferentially sulfonates cholesterol, while it has also significant activity with pregnenolone and DHEA (, ). Plays a role in epidermal cholesterol metabolism and in the regulation of epidermal proliferation and differentiation .
Sulfonates pregnenolone but not cholesterol.
Subcellular locations: Cytoplasm, Cytosol, Microsome, Nucleus
Phosphorylation of Ser-348 is required for translocation to the nucleus.
Expressed in the stratum granulosum-stratum corneum junction in the skin (at protein level) . Expressed highly in placenta, prostate and trachea and lower expression in the small intestine and lung .