Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway . Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities . Can also act independently of HSPA8/Hsc70: together with DNAJB12, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers . While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70 . When overexpressed, forms membranous structures together with DNAJB12 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear .
(Microbial infection) In case of infection by polyomavirus, involved in the virus endoplasmic reticulum membrane penetration and infection (, ).
Subcellular locations: Endoplasmic reticulum membrane, Nucleus membrane
Localizes to the endoplasmic reticulum membrane ( ). When overexpressed, forms membranous structures in the nucleus .
(Microbial infection) Upon SV40 infection, colocalizes with BCAP31, DNAJB12 and DNAJC18 in punctate structures within the endoplasmic reticulum membrane.