Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors . Involved in mitochondrial motility. When O-glycosylated, abolishes mitochondrial motility. Crucial for recruiting OGT to the mitochondrial surface of neuronal processes . TRAK1 and RHOT form an essential protein complex that links KIF5 to mitochondria for light chain-independent, anterograde transport of mitochondria (By similarity).
Subcellular locations: Cytoplasm, Nucleus, Mitochondrion, Early endosome, Endosome, Mitochondrion membrane, Cytoplasm, Cell cortex
Predominantly associated with early endosome. The localization to early endosomes depends on its interaction with HGS/HRS . Colocalizes with MGARP at the mitochondria .
High expression in spinal cord and moderate expression in all other tissues and specific brain regions examined. Expressed in all cell lines examined.