The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT . Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis . Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (By similarity). Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability (By similarity).
Subcellular locations: Cytoplasm, Nucleus, Chromosome, Centromere, Lateral cell membrane, Cell projection, Dendrite
Centromeric localization requires the presence of BUB1.