E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 ( , ). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (By similarity). The initial ubiquitin is then elongated (By similarity). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the ECS complex . Mediates 'Lys-6', 'Lys-48'- and 'Lys-63'-linked polyubiquitination ( ). May play a role in myelopoiesis .
Subcellular locations: Nucleus, Cytoplasm
Widely expressed with higher expression in granulocytes.