ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation ( ). Is required for efficient abscission during cytokinesis . Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells (, ). During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing . Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2 . Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST .
Subcellular locations: Cytoplasmic vesicle, Cytoplasm, Cytoskeleton, Microtubule organizing center, Centrosome, Midbody, Nucleus envelope
Localizes to centrosome and midbody of dividing cells ( ). Colocalized with SPART to the ends of Flemming bodies during cytokinesis . Localizes to the reforming nuclear envelope on chromatin disks during late anaphase.