Plays an important role in the control of DNA replication and the maintenance of replication fork stability ( ). Important for cell survival after DNA damage or replication stress . May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light . Forms a complex with TIMELESS and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress (  ).
Subcellular locations: Cytoplasm, Nucleus