Catalyzes the transfer of a sulfate group to position 3 of non-reducing beta-galactosyl residues in glycerolipids and sphingolipids, therefore participates in the biosynthesis of sulfoglycolipids (, ). Catalyzes the synthesis of galactosylceramide sulfate (sulfatide), a major lipid component of the myelin sheath and of monogalactosylalkylacylglycerol sulfate (seminolipid), present in spermatocytes . Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety . Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro) .
Subcellular locations: Golgi apparatus membrane
Expressed in kidney proximal tubule, gastric mucosa and adenocarcinoma (, ). Highly expressed in renal cell carcinoma cell lines (, ).