Isobutyryl-CoA dehydrogenase which catalyzes the conversion of 2-methylpropanoyl-CoA to (2E)-2-methylpropenoyl-CoA in the valine catabolic pathway ( ). To a lesser extent, also able to catalyze the oxidation of (2S)-2-methylbutanoyl-CoA (, ).
Subcellular locations: Mitochondrion
Detected at comparable levels in heart, lung, brain, skeletal muscle, pancreas and placenta. Weakly expressed in liver and kidney.