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FASTQ format is a text-based format for storing both a biological sequence -LRB- usually nucleotide sequence -RRB- and its corresponding quality scores .. format. File format. nucleotide sequence. nucleotide sequence. Both the sequence letter and quality score are each encoded with a single ASCII character for brevity .. ASCII. ASCII. It was originally developed at the Wellcome Trust Sanger Institute to bundle a FASTA sequence and its quality data , but has recently become the de facto standard for storing the output of high-throughput sequencing instruments such as the Illumina Genome Analyzer .. Wellcome Trust Sanger Institute. Wellcome Trust Sanger Institute. FASTA. FASTA format. Illumina. Illumina ( company )