There is a drama called Exit the King.
PAR-CLIP -LRB- photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation -RRB- is a biochemical method for identifying the binding sites of cellular RNA-binding proteins -LRB- RBPs -RRB- and microRNA-containing ribonucleoprotein complexes -LRB- miRNPs -RRB- .. biochemical. biochemistry. microRNA. microRNA. ribonucleoside. ribonucleoside. crosslinking. cross-link. The method relies on the incorporation of ribonucleoside analogs that are photoreactive , such as 4-thiouridine -LRB- 4-SU -RRB- and 6-thioguanosine -LRB- 6-SG -RRB- , into nascent RNA transcripts by living cells .. ribonucleoside. ribonucleoside. photoreactive. photosensitivity. Irradiation of the cells by ultraviolet light of 365 nm wavelength induces efficient crosslinking of photoreactive nucleoside -- labeled cellular RNAs to interacting RBPs .. photoreactive. photosensitivity. ultraviolet. ultraviolet. wavelength. wavelength. crosslinking. cross-link. labeled. flow tracer. Immunoprecipitation of the RBP of interest is followed by isolation of the crosslinked and coimmunoprecipitated RNA .. Immunoprecipitation. Immunoprecipitation. The isolated RNA is converted into a cDNA library and is deep sequenced using next-generation sequencing technology .. cDNA. complementary DNA. deep sequenced. deep sequencing. next-generation sequencing. next-generation sequencing. Recently , PAR-CLIP have been applied to determine the transcriptome-wide binding sites of several known RBPs and microRNA-containing ribonucleoprotein complexes at high resolution .. microRNA. microRNA