To investigate the interaction between FBXW7 and PEDV infection, the endogenous expression level of FBXW7was assessed after PEDV infection The results showed that PEDV infection inhibited the expression of FBXW7 in Vero E6 cells Then the effects of FBXW7 on PEDV replication was evaluated by western blot after transfection of pCAGGS-HA-FBXW7 to VeroE6 cells The results showed that PEDV N proteins were significantly suppressed by FBXW7 overexpression, indicating theinhibitory effect of FBXW7 on PEDV replication Further using fluorescent quantitative kit to detect the influence of overexpressingFBXW7 on interferon and interferon-stimulated genes under PEDV infection conditions, the results showed that compared with thepCAGGS-HA control group, The transcription level of IFN-beta, ISG15, ISG54 and ISG56 were significantly enhanced in host cellstransfected with pGAGGS-HA-FBXW7 (p0 05) Collectively, PEDV replication was facilitated by down regulating the expressionof FBXW7, which provides a novel strategy utilizing by PEDV to antagonize the host innate immune response
Porcine epidemic diarrhea virus antagonizes host antiviralresponse by downregulating the expression of FBXW7