This review gives an overview on the molecular diagnostic methods to detect the n-CoV disease with their limitations. The related articles are taken from different search engines like PUBMED, google scholar, MeSH Core, Cochrane, BioRxiv, Semantic scholar and so on that were published during the time period of 2000 to till date. Using the five step process of the selection such as identification of clear objectives, identification of relevant articles, selection of data extraction and charting, analysis and the report was generated. The inclusion criteria for this article is related to molecular diagnostic methods to detect n-CoV and the relevant publications are also included. Articles citing the general information of CoV’s were excluded. Among many methods suggested, RT-PCR is considered as the best method to detect the covid positivity. Detection of virus by RT-PCR in clinical specimens offers the best option of diagnosis in the early stages of the disease and its application also includes genotyping and mutation detection. Limitation of the RT-PCR method is its low sensitivity during the first few days of disease with much better sensitivity in later stages of the illness. This review highlights the suitable molecular detection methods for the n-CoV disease by analyzing their sensitivity and specificity together with the advantages and its limitations.
Diagnostic methods for covid detection-a review