Coronavirus has been determined to be the cause of the recent outbreak of severe acute respiratory syndrome (SARS) Human coronavirus 229E had been studied well and its receptor-binding domain was restricted to aa417–547 of S protein However, this region has no homology with the newly separated SARS-associated virus (Hong Kong isolate CUHK-W1) Then we analyzed the phylogenesis of S1 subunit of the coronavirus spike protein (SARS-associated virus, Hong Kong isolate CUHK-W1) Interestingly, the highest homology between murine hepatitis virus (MHV) and SARS-associated coronavirus was found And the important sites (aa62–65 and aa214–216) on the spike protein of MHV with receptor-binding capacity were highly conservative in comparison with the newly separated SARS-associated virus (the corresponding sites are aa51–54 and aal95–197) These results from bioinformatics analysis might help us to study the receptor-binding sites of SARS-associated virus and the mechanism of the virus entry into the target cell, and design antiviral drugs and potent vaccines FAU - Lu, Yun
Spike protein homology between the SARS-associated virus and murine hepatitis virus implies existence of a putative receptor-binding region