After a decade of experimental applications, it is the objective of this review to make a point on combinatorial peptide ligand libraries dedicated to low-abundance proteins from animal to plant and to microorganism proteomics. It is thus at the light of the recent technical developments and applications that we will examine the state of the art, its usage within the scientific community and its openness to unexplored fields. The improvements of the methodology and its implementation in connection with analytical determinations of CPLL-treated samples are extensively reviewed and commented upon. Relevant examples covering few critical aspects describe the performance of the technology. Finally a reflection on the technological future is attempted in particular by involving new concepts adapted to the limited availability of certain biological samples. This article is protected by copyright. All rights reserved.
Combinatorial peptides: A library that continuously probes low-abundance proteins.