Covid-19 detection in food is an effective solution to ensure the accurate detection rate of Covid-19. The difficulties and detection methods of food virus safety detection and the feasibility of digital PCR detection technology are analyzed. The main parameters and characteristics of dPCR technology and other PCR technologies are compared. The application of dPCR technology in the detection of food viruses and pathogenic bacteria, the application of dPCR technology in the preparation and purity verification of Covid-19 RNA reference material, and the steps and methods of dPCR technology in food testing Covid-19 were expounded. Compared with traditional detection methods, digital PCR technology has great advantages in virus detection limit and stability. dPCR will develop towards high flux and automation, and achieve the absolute quantification of multiple target sequences at low cost. It will help to play a crucial role in the detection of covid-19 in food. © 2021 The Authors, published by EDP Sciences.
Application of digital PCR (dPCR) in the detection of Covid-19 in food