We optimized a fluorescent quantitative polymerase chain reaction (qPCR) assay system for rapid and real time detection of SARS-CoV-2 RNA The results show that the lowest dilution of RNA samples used for the detection of SARS-CoV-2 RNA could reach 1/10 000 (the initial value is set as 10 ng/muL) Moreover, the cycle threshold (Ct) for samples of clinically diagnosed COVID-19 was lower than 35 or 40 The sensitivity of this method was satisfactory The results were consistent with those of the COVID-19 detection kit on the market under the same conditions, but the number of cycles required was shortened by about 2 Therefore, the optimized assay developed in this study can be used in screening and early clinical diagnosis Our work provides a tool to facilitate rapid clinical diagnosis of COVID-19
[Optimization of a fluorescent qPCR detection for RNA of SARS-CoV-2]