Qingfei Paidu Decoction is a Chinese medicine formula which has been proved effective in the treatment of Coronavirus Disease 2019. However, the comprehensive separation and characterization of Qingfei Paidu Decoction is of great challenge due to the diversity of chemical components in a wide range of polarity. In this study, a triplex off-line two-dimensional liquid chromatography (LC) coupled with quadrupole time-of-flight mass spectrometry (MS) is developed for the analysis of Qingfei Paidu Decoction. One reversed phase LC×hydrophilic interaction LC system and two reversed phase LC×reversed phase LC systems were constructed to separate polar components and weak-polar components in Qingfei Paidu Decoction, respectively. Benefiting from the good orthogonality of two-dimensional LC and high sensitivity of quadrupole time-of-flight MS, chemical components with different polarities and content were discovered. A total of 749 peaks were detected in positive and negative ionization mode, and presented as a four-dimensional data plot. Meanwhile, 498 compounds belonging to 14 categories were tentatively identified. These results provide good supplementary to elucidate the material basis of Qingfei Paidu Decoction. The triplex off-line two-dimensional LC-quadrupole time-of-flight MS strategy can be a powerful and efficient tool for the separation and characterization of chemical substances in traditional Chinese medicine formulas. This article is protected by copyright. All rights reserved.
Chemical profiling of Qingfei Paidu Decoction by triplex off-line two-dimensional liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.