Acrachne racemosa (B. Heyne. ex Roem. & Schult.) Ohwi of the subfamily Chloridoideae of the family Poaceae is an economically important grass species. Grasses are characterized by deposits of silica in the cells or tissues in the form of phytoliths which protect them from various types of biotic and abiotic stresses. Owing to variable shape and specificity of morphotypes, phytolith helps in taxonomical studies, reconstruction of paleoenvironments and prediction of climate changes. The present study focussed on developing a phytolith profile of the selected species. For isolation of phytolith, Dry Ashing Method was employed, and by epidermal peeling, in-situ location of phytoliths was deciphered. In the present study, silica percentage was studied from different parts of the plant and the maximum amount was found in the leaf. Frequency and morphometric data of phytolith morphotypes from different parts of the plants were also collected and analyzed. The strongest correlation was found between phytolith types of root and culm by Pearson's correlation coefficient supported by cluster analysis. The saddle type of phytoliths had the highest frequency in the leaf; other types of phytoliths in different parts of the plant were bilobate, blocky types, elongate types, trapezoids, triangular, cross, sinuate elongate, tabular types, globular types. Functional groups and amorphous polymorphic phases of silica were also analyzed by FTIR and XRD. It was concluded that phytolith types are controlled by parts of plant body and by anatomical and environmental factors.
Phytolith profile of Acrachne racemosa (B. Heyne ex Roem. & Schult.) Ohwi (Cynodonteae, Chloridoideae, Poaceae)