The novel human coronavirus (SARS-CoV-2) causes the coronavirus disease 2019 (COVID-19) pandemic worldwide Control of COVID-19 pandemic is vital for public health and is the prerequisite to maintain social stability However, the origin and transmission route of SARS-CoV-2 is unclear, bringing huge difficult to virus control Monitoring viral variation and screening functional mutation sites are crucial to prevention and control of infectious diseases In this study, we developed a user-friendly software, named BioAider, for quick sequence annotation and mutation analysis on large-scale genome-sequencing data Herein, we detected 14 substitution hotspots within 3,240 SARS-CoV-2 genome sequences, including 3 groups of potentially linked substitution NSP13-Y541C was crucial substitution which might affect the unwinding activity of helicase In particular, we discovered a SR-rich region of SARS-CoV-2 distinct from SARS-CoV, indicating more complex replication mechanism and unique N-M interaction of SARS-CoV-2 Interestingly, the quantity of SRXX repeat fragments in SARS-CoV-2 provided further evidence of its animal origin Overall, we developed an efficient tool for rapid identification of viral genome mutations and could facilitate the viral genomic study Using this tool, we have found critical clues for the transmission route of SARS-CoV-2 which would provide theoretical support for the epidemic control of pathogenic coronaviruses
BioAider: an efficient tool for viral genome analysis and its application in tracing SARS-CoV-2 transmission