Additive manufacturing technology, referred as 3D printing technology, is a growing research field with broad applications from nanosensors fabrication to 3D printing of buildings. Nowadays, the world is dealing with a pandemic and requires the use of simple sensing systems. Here, the strengths of fast screening by a lab‐on‐a‐chip device through electrochemical detection using 3D printing technology for SARS‐CoV‐2 sensing are combined. This system comprises a PDMS microfluidic channel integrated with an electrochemical cell fully 3D‐printed by a 3D printing pen (3D‐PP). The 3D‐PP genosensor is modified with an ssDNA probe that targeted the N gene sequence of SARS‐CoV‐2. The sensing mechanism relies on the electro‐oxidation of adenines present in ssDNA when in contact with SARS‐CoV‐2 RNA. The hybridization between ssDNA and target RNA takes a place and ssDNA is desorbed from the genosensor surface, causing a decrease of the sensor signal. The developed SARS‐CoV‐2/3D‐PP genosensor shows high sensitivity and fast response.
3D‐Printed SARS‐CoV‐2 RNA Genosensing Microfluidic System