Human eccrine sweat contains numerous biomarkers which can provide information on health,performance, and aging. Non-invasive collection and measurement of biomarkers has becomeespecially important in recent times given viral outbreaks like SARS-CoV-2. In the current studywe describe a method of sweat collection from palmar surfaces in participants via surface captureusing glass beads and the resulting analysis of biomarkers from very low volumes of sweat usingliquid chromatography mass spectrometry with selected ion monitoring. Study participantsunderwent a cognitive and physical stress task with easy and hard conditions with sweat beingcollected after each task. Resulting analysis found a signal for 22 steroid biomarkers and wereport detailed information on selected biomarkers, given their applicability to timely real-worldexemplars, including cortisol, dehydroepiandrosterone, allopregnanolone, estrone, aldosterone,and 20a/b-dihydrocortisone.
LCMS Measurement of Steroid Biomarkers Collected from Palmar Sweat.