A rapid modified gas chromatographic assay for esterase activity.
A rapid modified procedure for the gas chromatogrpahic determination of esterase activity was studied. Aliphatic esters such as ethyl n-butyrate, n-propul n-butyrate, n-butyl n-butyrate and n-amyl n-butyrate were used as substrates and acetone was chosen as the most suitable solvent for dissolving the substrates in order to avoid alcoholysis. The enzyme reaction was started in a mixture of 0.03 M phosphate buffer, pH 7.90, containing an adequals, an aliquot of the reaction solutionwas injected directly on to a gas chromatograph and the alcohols produced were separated.
PubMed Abstracts
PubMed Abstracts