[The adenine riboswitch: a new gene regulation mechanism].
It has long been known that gene regulation is mostly achieved via protein-nucleic acid interactions. However, the role of RNA factors in gene control has been recently growing given the implication of new RNA-based gene regulation mechanisms such as microRNAs and related short-interfering RNAs gene expression inactivation mechanisms. Recent studies have demonstrated that the involvement of RNA in fundamental gene-control processes is even more extensive. Prokaryotic messenger RNAs carry highly structured domains known as riboswitches within their 5'-untranslated regions. Each riboswitch is able to bind with high specificity their cellular target metabolite, without the involvement of a protein cofactor. Upon metabolite binding, the messenger RNA undergoes structural change that will ultimately lead to the modulation of its genetic expression. Riboswitches can alter gene expression at the level of transcription attenuation or translation initiation, and can up- or down-regulate gene expression by harnessing appropriate changes in the mRNA structure. Here, we provide an overview of the adenine riboswitch, one of the smallest riboswitch and one of the few that activates gene expression upon ligand binding. Several crystal structures have been obtained for the ligand-binding domain of this riboswitch providing us with an unprecedented glimpse about how riboswitches use their ligand to regulate gene expression. Moreover, mechanistic studies have recently shed light on the transcriptional regulation mechanisms of the adenine riboswitch suggesting that riboswitches may rely on the kinetics of ligand binding and the speed of RNA transcription, rather than simple ligand affinity. Riboswitches are particularly interesting because RNA-ligand interactions are potentially very important in the elaboration of antimicrobial agents.
PubMed Abstracts
PubMed Abstracts