The inhibitory function of icariin in cell model of benign prostatic hyperplasia by upregulation of miR-7.
In spite of a considerable breakthrough in antitumor effects of icariin (ICA), the studies regarding other functions of ICA still have not explored widely. This study aimed to report the function of ICA about the growth and migration of benign prostatic hyperplasia epithelial cell line (BPH-1) and human prostate epithelial cell line RWPE-1. Both BPH-1 and RWPE-1 cells were treated with ICA for 12 hr. The cells proliferation, migration, apoptosis were tested by CCK-8 assay, transwell assay, flow cytometry, and western blot. The miR-7 expression was silenced by transfection in both BPH-1 and RWPE-1 cells. The activation of PI3K/AKT and Raf1/ERK1/2 signaling pathways was detected by western blot. ICA inhibited both BPH-1 and RWPE-1 cells proliferation, migration, and promoted apoptosis. Meanwhile, ICA promoted miR-7 expression. Other than this, the changes of cells proliferation inhibition, migration inhibition and apoptosis promotion in both BPH-1 and RWPE-1 cells induced by ICA were reversed partially or completely by transfecting specific miR-7 inhibitor. Apart from this, the activation of PI3K/AKT and Raf1/ERK1/2 signaling pathways was inhibited by ICA through upregulating miR-7 expression. The study released that ICA inhibited both BPH-1 and RWPE-1 cells proliferation, migration and promoted apoptosis by upregulating the levels of miR-7 expression, which was related to PI3K/AKT and Raf1/ERK1/2 signaling pathways.
PubMed Abstracts
PubMed Abstracts