Increase in cytosolic Ca2+ concentration during t-butyl hydroperoxide metabolism by isolated hepatocytes involves NADPH oxidation and mobilization of intracellular Ca2+ stores.
Activation of phosphorylase a in hepatocytes incubated with t-butyl hydroperoxide indicates that hydroperoxide metabolism is associated with an increase in cytosolic free Ca2+ concentration which appears to be mediated by NADPH oxidation and to involve mobilization of intracellular Ca2+ stores.