chromosomes are not packed randomly in the nucleus the rabl orientation is an example of the non random arrangement of chromosomes centromeres are grouped in a limited area near the nuclear periphery and telomeres are located apart from centromeres this orientation is established during mitosis and maintained through subsequent interphase in a range of species we report that a rabl like configuration can be formed de novo without a preceding mitosis during the transition from the sexual phase to the vegetative phase of the life cycle in fission yeast in this process each of the dispersed centromeres is often associated with a novel sad1 containing body that is contacting a cytoplasmic microtubule laterally sad1 is a component of the spindle pole body spb the sad1 containing body was colocalized with other known spb components kms1 and spo15 but not with cut12 indicating that it represents a novel spb related complex the existence of the triplex structure centromere microtubule sad1 body suggests that the clustering of centromeres is controlled by a cytoplasmic microtubular system accordingly when microtubules are destabilized clustering is markedly reduced.