Contributions to silica gel application in residue analysis: V. Simple micro method for liquid chromatographic separation of chlorinated hydrocarbon pesticides and polychlorinated Biphenyls (PCB's)
A simple micromethod for the determination of organochloride pesticides and polychlorinated biphenyls (PCB's) is discussed. Using 2 g, 3 g and 4 g silica gel columns, the pesticides are quantitatively eluated with 20 ml, 25 ml and 30 ml of solvent, retaining 90 mg, 140 mg and 190 mg of fat. Recoveries of pesticides ranged from 90–100%.
The establishment of reliable serological methods for cysticercosis in pigs is important for the surveillance, control and prevention of taeniosis/cysticercosis in humans as well as in pigs to prevent economic loss. Both ELISA and immunoblot using glycoproteins (GPs) purified by a single step of preparative iso-electric focusing, which are highly useful for human cysticercosis, have been applied for a serological study in pigs naturally infected with Taenia solium . All sera from pigs showed similar responses to those in human cysticercosis. Therefore, it is expected that both ELISA and immunoblots using GPs would be useful in differentiating infected pigs from uninfected ones.