The nature of the breakdown of thyroglobulin
Thyroglobulin consists chiefly of a component of M.W. 660,000 and sedimentation coefficient 19 S. On complete reduction of the 101 disulphide bonds, subunits are obtained (TgM) with M.W. ca. 165,000 [l] . However, these slowly give rise to products of lower M.W. [2] even when stored at -20” [3]. In a previous paper from this laboratory [4] we described the slow breakdown of TgM at pH 8.7 in the presence of SDS, leading to the formation of two products, B and C, with M.W. 35,000 and 20,000 respectively. In the present communication we will describe further breakdown products, their composition and conditions which favour their formation.
ABSTRACTPurpose.The purpose of this study was to develop a novel, quantitative measurement of tear film breakup dynamics (TBUD) to study the phenomenon of tear breakup in dry eye and control subjects and its impact on dry eye symptoms.Methods.Ten control and 10 dry eye subjects completed the Dry Eye