RNA isolation from high-phenolic freeze-dried tea (Camellia sinensis) leaves
With minor modifications, we applied a previously reported RNA isolation protocol that used guanidine hydrochloride to leaves of lyophilized (freeze-dried) tea (Camellia sinensis). Plant tissue must be preserved in its collected state, especially when genome-wide expression profiles are studied. Fresh leaf tissues cannot feasibly be transferred at ultra-low temperatures from natural habitats to the laboratory. We explored the use of lyophilized tissue for RNA isolation from tea leaves. High yields of RNA (∼500 μg/g dry weight of leaf tissue) were obtained, and the RNA was suitable for all molecular biology methods tested, including Northern blotting, reverse transcription, and microarray analysis. We demonstrated that RNA obtained from freeze-dried leaf tissue was high quality, undegraded, and useful for all downstream applications.
Objective To study the best extracting technology of Compound Taizishen Granule prescription.Methods The total polysaccharides and total saponins were determined by UV spectrophotometer.With the content of total polysaccharides and total saponins as comprehensive indexes,orthogonal test was applied to investigation of the water addition,extraction time,and extraction times for optimizing the best extracting technology.Results The best extracting technology was using 10 times the amount of water,extracting 3 times and 1.5 h per time.Conclusion The extraction technology is safe and effective,which can be used as extraction for Compound Taizishen Granule prescription.