Dehydrogenase-silica as a stationary phase for the separation of alcohols and ketones
Abstract Horse liver alcohol dehydrogenase was immobilized on tresyl-activated silica particles and slurry packed into a PTFE-coated stainless-steel column. The stationary phase enzyme column was used for the high-performance chromatographic separation of alcohols and ketones. Baseline resolution of the secondary alcohols terbutaline and bambuterol and their respective ketones was obtained. In addition, discrimination of closely related isomers, including the positional isomers of ethylphenethyl alcohol and the optical isomers of 1-phenyl-1-butanol, was achieved.
The establishment of reliable serological methods for cysticercosis in pigs is important for the surveillance, control and prevention of taeniosis/cysticercosis in humans as well as in pigs to prevent economic loss. Both ELISA and immunoblot using glycoproteins (GPs) purified by a single step of preparative iso-electric focusing, which are highly useful for human cysticercosis, have been applied for a serological study in pigs naturally infected with Taenia solium . All sera from pigs showed similar responses to those in human cysticercosis. Therefore, it is expected that both ELISA and immunoblots using GPs would be useful in differentiating infected pigs from uninfected ones.