Establishment and optimization of AFLP technology system used to mark characteristics of apple
The AFLP(Amplified Fragment Length Polymorphism) analysis system was established suited for marking agronomic characteristics of apple with the population of F_1 progenies of "Maypde"× "Muls".Common questions in the analyzing procedure including the preparation and purification of genomic DNA,restriction-ligation of DNA,pre-amplification and selective-amplification of the products of restriction-ligation,and electrophoresis on denaturing polyacrylamide gels,and their corresponding solutions were studied and summarized.The results indicated that clear genetic marker map could be drawn out with this AFLP analysis system,and the results were steady and credible,and could be easily repeated.
e18521Background: Acute Promyelocytic Leukemia (APL), a subtype of acute myeloid leukemia, has excellent outcomes, but continues to show high rates of early mortality. An epidemiologic study utiliz...