is lysozyme a monomer
The protein with a molecular weight of 28.6 kDa in lysozyme solution, which has been recognized as a lysozyme dimer, was purified and its association was observed using time-resolved static light scattering and dynamic light scattering under the same buffer condition as that used in lysozyme monomer association.easurements of TR-SLS and dynamic light scattering for a lysozyme dimer solution showed that the association behavior is affected by the purity of the dimer solution. Chromatography and SDS–PAGE analysis showed two types of dimers, strong-and weak-bonded molecules, in a dimer unit.
A double emulsion solvent evaporation process was used to produce the microspheres used in this study but any method of production is suitable for this approach. The protein lysozyme was used as a model protein therapeutic for release from the PLGA microspheres.