[Effects of diazoxide on the mitochondrial membrane potential and ROS generation in rat uterus cells].
In the present study we demonstrate partial depolarization of the mitochondrial inner membrane from the rat uterus cells upon activation of mitochondrial ATP-sensitive K(+)-channel (mitoK(ATP)) with diazoxide. The estimated affinity constant of diazoxide to mitoK(ATP) from rat uterus cells is (5.01 +/- 1.47) 10(-6) M. We also observed an enhanced generation of reactive oxygen species after addition of diazoxide. Both effects were effectively eliminated by glybenclamide, blocker of the ATP-sensitive K+ channel. Our results indicate that activation of mitoK(ATP) in rat uterus cells leads to a partial depolarization of mitochondrial membrane and an increase in ROS concentration.
Abstract In order to study the size distribution of a radioactivity due to radon daughters in the environmental atmosphere, an experimental procedure using filters, ion tubes, diffusion batteries and cascade impactors (CASELLA MK2 and ANDERSEN) is presented. A comparison between the experimental results and a theoretical curve, deduced of the attachment theory of the small radioactive ions on atmospheric particles, is carried out.