A Selective Medium for Bacillus anthracis
Summary: A medium containing propamidine is described which has high selective activity for the species Bacillus anthracis. The spore form of the organism is essential as inoculum for the medium.
Despite the prevalence of microfluidic-based heterogeneous immunoassays, there is incomplete understanding of analyte capture parameters. This study presents computational results and corresponding experimental binding assays of analyte capture. Our results identify: 1) a “reagent-limited” regime, under constraints of finite sample volume and assay time; 2) a critical flow rate; 3) an increase in signal by using a short concentrated plug rather than a long dilute plug; 4) a requirement to eventually reach a reaction-limited operating regime to maximize the capture of analytes [1].