Inhibition effect against tyrosinase of condensed tannins from Korean green tea.
For the utilizing of tannins in the functional foods and natural inhibitor against browning reaction by tyrosinase in foods, inhibition effect against tyrosinase of tannins from Korean green tea was determined. Acetone extract from Korean green tea showed inhibition effect against tyrosinase. The gallocatechin compounds showed higher inhibition effect than the catechin compounds. In terms of stereo isomers, (-)-epicatechin compounds had higher inhibition effect than the (+)-catechin compounds. The monomer had higher inhibition effect than the dimer.
Objective To study the best extracting technology of Compound Taizishen Granule prescription.Methods The total polysaccharides and total saponins were determined by UV spectrophotometer.With the content of total polysaccharides and total saponins as comprehensive indexes,orthogonal test was applied to investigation of the water addition,extraction time,and extraction times for optimizing the best extracting technology.Results The best extracting technology was using 10 times the amount of water,extracting 3 times and 1.5 h per time.Conclusion The extraction technology is safe and effective,which can be used as extraction for Compound Taizishen Granule prescription.