Inhibition of nemin-induced trap formation in a predaceous fungus by 6-methyl purine.
Abstract The RNA synthesis inhibitor 6-methyl purine (5 × 10 −3M ) completely suppressed nemin-induced trap formation in the predaceous fungus Monacrosporium doedycoides . Lower concentrations of the inhibitor resulted in proportional inhibitions of trap formation. Traps were also inhibited by compounds affecting protein synthesis i.e. 5-fluorouracil and p-flurophenylalanine. The kinetics of 6-methyl purine inhibitions suggested that the nemin had an immediate effect on RNA synthesis in this predaceous fungus. Such initial data provide indirect evidence that the action of nemin in the induction of fungal traps may reside at the level of transcription.
OBJECTIVE To study the effect of solvents on the permeation of leflunomide in excised mouse skin. METHODS Modified Franz-diffusion cell adopted as apparatus for in vitro skin permeation, excised mouse skin as permeation barrier and permeability as index, the concentration of leflunomide in the samples was measured by HPLC. RESULTS Ethanol has enhancement effect on the permeability of leflunomide, propylene glycol and lower concentration DMI have little effect on the permeability of leflunomide. CONCLUSIONS These solvents can be used in the skin local preparations of leflunomide.