Optimization of the Rapid Purity Identification System of Hybrid Rice Seeds by Using SSR Markers
To overcome the limiting factors in the commercialization of the existing rapid purity identification system of hybrid rice seeds by using SSR markers,the studies were conducted on the improvement of the technology system in the aspects of DNA extraction,PCR amplification procedure and gel electrophoresis.After optimization,the time for DNA extraction,PCR amplification and gel electrophoresis was decreased from 300,200 and 180~210 minutes to 2.5,65 and 60 minutes,respectively.Meanwhile,the cost was greatly reduced and the pollution to the environment was abated by cutting down the dosage of dNTP and Taq DNA polymerase,and reusing the gelose gel.Therefore,an optimized accurate,simple,rapid and low costing technical system of fast purity identification of hybrid rice seeds was established.
The presented study is an eye tracking experiment for high-resolution satellite (HRS) images. The reported experiment explores the Area Of Interest (AOI) based analysis of eye fixation data for complex HRS images. The study reflects the requisite of reference data for bottom-up saliency-based segmentation and the struggle of eye tracking data analysis for complex satellite images. The intended fixation data analysis aims towards the reference data creation for bottom-up saliency-based segmentation of high-resolution satellite images. The analytical outcome of this experimental study provides a solution for AOI-based analysis for fixation data in the complex environment of satellite images and recommendations for reference data construction which is already an ongoing effort.