A modified rabbit ear chamber and an example of its application for intravital-microscopic study on acute effects of topical thermal stimulation.
Abstract A pre-existing plastic rabbit ear chamber was modified to be applicable for intravital-microscopic observations in pharmacological as well as pathophysiological studies on inflammation. The chamber consists of a base disk, a mica (or glass) cover-slip and a holder ring. The base disk has a round-table with a central protrusion in which a heat conductor of platinum wire is so implanted near the protrusion that thermal stimulation can be given to the regenerated tissue between the table and the coverslip.
Aiming at Shenyang Metro in construction,analysis of influence of advance small duct grouting on metro tunnel excavation is conducted.By different layout in ring of small duct,based on surrounding rock stress and displacement of each monitored point of tunnel,the rational layout is given.which has important reference meaning to similar tunnel engineering.