STRUCTURAL PROPERTIES OF STAPHYLOCOCCAL NUCLEASE IN OLIGOMERIC A-FORMS
Association affects the structural properties of different partially folded conformations of staphylococcal nuclease induced by anions of different nature. It is shown that oligomerization induces new structural levels in non-native A-forms. A close structural similarity between the monomeric A2 and the dimeric (A1)2 forms as well as between the monomeric A3 and oligomeric [(A1)2]M and [A2]M forms is established. This suggests that association of a protein molecule in partially folded conformations can be an additional structure forming factor.
In this study, a doubled-haploid population with 115 individuals derived from a cross of Yolo wonder×Perennial were test-crossed by 77013A, a stable germ male sterile line, to construct a genetic map and locate QTLs of fertility restorer. Three methods were adopted to investigate the fertility of each line of the tested population. Among 128 AFLP primers pairs, 61 amplified polymorphisms between two parents were found. The map, as the results, included 8 linkage groups and 43 markers. Based on the investigation of fertility of the tested population, 4 main QTLs were located on linkage group 1,3,6,8, respectively.