purification of e coli poly a polymerase
Received July 13, 1990. DNA polymerase from Thermus aquaticus has be-. come a common reagent in molecular biology because of. its utility in DNA amplification and DNA sequencing. protocols. A simplified method is described here for iso-. lating the recombinant Taq enzyme after overproduc-. tion in Escherichia coli. Purification requires 8 to 10 h. and entails heat treating and clearing the E. coli lysate, followed by precipitation of the enzyme with polyethy-.
Removal of Brilliant Green from wastewater using conventional and ultrasonically prepared poly(acrylic acid) hydrogel loaded with kaolin clay: A comparative study. Shirsath SR, Patil AP, Patil R, et al.Ultrason.Sonochem. synthesizing poly(N-isopropylacrylamid e)-block-PAA copolymer which responds to both temperature and pH stimuli 2. â¢ in preparing block copolymer of oligo (methyl methacrylate)/PAA for micellar delivery of hydrophobic drugs 3. â¢ as thickening agent for adhesives.