Clinical application of genetic polymorphism in neurofibromatosis type 1.
The authors report the study of DNA polymorphic sequences, 5 intragenic and 5 flanking the NF1 gene, in 87 Italian NF1 families for a total of 142 affected individuals and 204 non-affected relatives. All PCR-based analyses are easy and simple to perform, and require small amounts of DNA. The non radioactive method used is sensitive, rapid, and has low background. All subjects were informative for at least 2 markers. The use of linkage study to familial cases allowed us to exclude the diagnosis prenatally in two fetuses, and to confirm or exclude diagnosis in those relatives with clinical signs, but not fulfilling the international diagnostic criteria. Furthermore indirect analysis permitted the detection of large gene deletions by loss of heterozygosity of one or more DNA markers in three out of 47 sporadic cases.
This study concentrated on the production of molecularly imprinted polymers (MIPs) as highly selective sorbents for felodipine (FLD), a representive dihydropyridine calcium antagonists.Demonstrated chromatographically through a selection factor, these MIPs showed high selectivity for the template molecule among a group of structurally similar compounds. The recognition was found to correlate with structural similarity to the template compound.