Effects of Egg Woman (Phyllanthus amarus) Extract on Complement C3 Expression in Nile Tilapia (Oreochromis niloticus) by Real Time RT-PCR Technique
Effects of the crude extract from Egg woman (P . amarus ) on Complement C3 gene expression in liver and spleen of Nile tilapia (O. niloticus ) by Real time Reverse Transcriptase Polymerase Chain Reaction (Real time RT-PCR). Experimental fish 15-20 g initial weight were fed for 3 week on diet containing P. amarus crude extract at 0 (control), 1, 5, and 10 g/kg feed. The analysis of data by ANOVA showed non-significant in every groups (p>0.05). The results indicated that 5 g/kg Feed of Egg woman extract tend to stimulate the Complement C3 gene expression higher than other dose. The result from this study showed the benefit of Egg woman extract that can be used to improve the immune system of Nile tilapia.
OBJECTIVE:To establish the quality control standard of Xuebao Capsules. METHODS:Applying the technique of TLC to identify the main ingredients Fructus Psoraleae , Radix Polygoni multiflori prepared, Pericarpium Citri reticulatae, Radix Ginseng, Radix Astragali by using chemical reference substance or reference crude drug.RESULTS: Fructus Psoraleae, Radix Polygoni multiflori prepared, Pericarpium Citri reticulatae , Radix Ginseng , Radix Astragali could be detected by TLC.CONCLUSION:The methods were practical, special and reproducible.