We have developed a novel technique for cell sheet formation and continuous cell culture without the need for frequent subculture that make use of open-mesh scaffold suspended in a culture medium. Epithelial cells seeded on the scaffold underwent elongation and proliferation normally, expanding dynamically to form a monolayer cell sheet over the meshed scaffold. Cytoskeletal labeling revealed extensively interconnected meshwork of the actin cytoskeleton, suggesting that the mechanical strength of the cell sheets was due to cell-cell adhesion. Thus the method provides a simple approach to cell sheet generation without the use of expensive smart polymers.
We have developed a novel method for fabricating monolayer cell sheets by culturing cells on microstructured mesh sheets suspended in a culture medium as substrates for cell attachment and growth. In this study, we evaluated the relationship between cell orientation and mesh shape with the aim of generating cell sheets with defined cell orientation. When cultured on mesh sheets with a diamond shape, fibroblasts reorganized to form monolayer cell sheets with cells aligned primarily in the direction of the major axis. Thus, by modulating mesh shape, it is possible to fabricate mesh sheets with controlled cell orientation for use in tissues/organs such as the skeleton and heart muscles where cell alignment is particularly critical to the realization of proper tissue/organ function.
MLL1 regulates circadian promoters by depositing H3K4 trimethyl marks, whose levels are also modulated by the NAD+-dependent deacetylase SIRT1. SIRT1 is now shown to promote circadian deacetylation of MLL1, thus affecting MLL1's methyltransferase activity.