Antibody response of mice to chemically induced tumors ( murine leukemia virus / tumor-specific transplantation antigen / methylcholanthrene-induced sarcoma )
Abstract ::: BALB/c mice immunized with syngeneic methylcholanthrene-induced fibrosarcomas did not have antibodies against the unique tumor-specific transplantation antigens, even though they were capable of rejecting a lethal dose of tumor cells. Endogenous murine leukemia virus antigens expressed by certain of the tumors did, however, elicit high titers of antibodies, accounting for serological crossreactions that occurred between those tumors.
Objective To investigate the effect of the expression of laminin on the expression of LM receptor and focal adhesion kinase stimulated by transforming growth factorβ. Methods The laminin binding receptors was identified by  125 I-laminin saturation binding assays. Western blot technique was used to study the expression of FAK at protein level with ECL reagents. Results The expression of LM receptor stimulated by TGFβ was down regulated by the expression of antisence laminin RNA. No change was found in the expression of FAK when stimulated by TGFβ, but its expression was down regulated with the help of laminin antisense RNA. Conclusion The expression of LM receptors and FAK could be regulated when stimulated by TGFβ and LM takes part in the regulation of FAK.