Anomerization of glucose 6-phosphate: pH dependence and solvent isotope effects☆
Abstract The anomerization of α- d -glucose 6-phosphate has been examined using a spectrophotometric coupled enzyme assay. The pH-rate profile for spontaneous d -glucose 6-phosphate anomerization reveals that the d -glucose 6-phosphate dianion is the species giving rise to the much higher rate of d -glucose 6-phosphate anomerization over that of d -glucose. A deuterium solvent isotope effect of k H 2 O k D 2 O = 1.7 is consistent with the postulated intramolecular general-base catalysis by the phosphate.
Background and Objective Chemotherapeutic drug treatment outcomes are genetically determined. Polymorphisms in genes encoding phase II drug metabolizing enzyme glutathione-S-transferase (GST) can possibly predict treatment outcomes, and can be of prognostic significance in breast cancer patients. The aim of this study was to determine the role of genetic variations in GST in predicting response to, and toxicity of, anthracycline-based chemotherapy in breast cancer patients. Method Two hundred and seven patients treated with anthracycline-based chemotherapy were genotyped for GSTM1 and GSTT1 deletion polymorphisms, and GSTP1 Ile 105 Val (rs1695), by polymerase chain reaction (PCR)/