An Improved Method of Elimination of DNA from PCR Reagents
Objective: The presence of exogenous DNA in commercially available polymerase chain reaction (PCR) reagent preparations is a serious problem when amplifying conserved regions of bacteria. The preferred and currently in-use method of decontamination using 8-methoxypsoralen (8-MOP) and UVA requires re-standardization of decontamination with increasing concentrations of 8-MOP and UVA irradiation timings, if the DNA load of reagents is high due to lot-to-lot differences. The objective of this study was to develop a decontamination method, which would (i) work at the minimum reported concentration of 8-MOP and UVA irridation timings; and (ii) take care of inter-batch DNA-load variability of reagents.
Visibility in the rain is poor. Here, we develop a rain removal system for dynamic scene in diminished reality. Our system provides a clear and comfortable view. We apply and extend chromatic prior and explore chromatic pair, dark tail removal and spatial chromatic prior. We design an optimized intuitive alignment to solve moving camera problem. We apply anti-flicker to completely remove drizzle and defocused rain. Benefit from parallel pixel-based chromatic prior, our system has high scalability and extensibility. Our experiments reference numerous videos from prior related works, involving realistic and diverse property of rain, motion of the scene and motion of the camera. The experimental results show a satisfactory rain removal quality, especially for drizzle and defocused rain.