Interaction study of Glutamic Acid with double-stranded calf thymus DNA by using Cyclic Voltammetric, Ultraviolet-visible and Fluorescence spectroscopy
Interaction of glutamic acid (Glu) was studied with double-stranded (ds) calf thymus DNA (CT-DNA) by cyclic voltammetry (CV) then obtained results were further correlated by ultraviolet-visible (UV-Vis) and fluorescence spectroscopies. Calculated binding constant from all techniques was very close to each other and it is around 3.54× 10 3 M L -1 . Further binding sites were also calculated and it is near to 1, which indicates appropriate binding of Glu with CT-DNA. The result shows Glu binds in groove modes of DNA. Further binding free energy ( ∆ G) of the complex was also calculated and it is -4.76 kCal M -1 . In our work a correlative intractability pattern for Glu with DNA has been identified.
Author contributions ::: ::: GH helped design the study, establish the animal model, collect and analyze samples, and write the manuscript. YY and DH helped establish the animal model, performed Western blotting, immunohistochemistry analyses. YY and DH also helped analyse data and revise the manuscript. QYW and JK contributed to study planning, analyse and interpret data, review and revise the manuscript. All authors have approved the final version of this article to be published and agreed to be accountable for all aspects of the work in ensuring the questions related to the accuracy or integrity of any part of the work. ::: ::: ::: ::: Disclosure ::: ::: The authors report no conflicts of interest in this work.