limitations of bridging elisa assays
Practical aspects of Ab assays Each Ab assay has its own characteristic advantages and disadvantages based on technical difficulty, intra- and interassay variability (i.e., precision), sensitivity for measuring Abs, specificity for the target protein or ligand, susceptibility to false-positive or false-negative results, and cost.
It also helps other researchers reconstruct a study or advance future research on the same topic. Delimitations provide the scope within which researchers conclude findings and determine a study’s reliability or external validity.Limitations are elements of a study that are not under the control of the researcher. Examples of limitations include funding and time constraints that affect methodology.