piggyBac-mediated generation of stable transfectants with surface human leukocyte antigen expression from a small number of cells.
Techniques useful for isolating a series of stable transfectants from a small number of cells are important for high-throughput functional analyses of genes of interest (GOIs). This study employed piggyBac transposon vectors carrying an IRES-eGFP cassette to isolate stable transfectants. The 14 transfections performed produced stable transfectants; a small number of cells (5.7×10(4)) was sufficient even when liposomes were used to transfect non-adherent cells. Of note, there was a close relationship between eGFP fluorescence intensity and GOI (human leukocyte antigen) expression level. Therefore, the current system would be useful for the high-throughput acquisition of clones showing high GOI expression.
By promoting the production method of obtaining a gene function in a medium containing acetic acid from an acetic acid bacterium chromosomal DNA library, part of the practice of the genus Gluconobacter acetate acetic acid bacterium having cloned on a practical level acetic acid fermentation to improve the function of new genes. The gene was introduced into an acetic acid bacterium to obtain a transformant, culturing the transformant that when in the presence of ethanol, it is possible to significantly shorten the growth induction period, and significantly improve the rate of acetic acid fermentation.