Identification and expression of the GTcyc cDNA clone from genetic tumors formed on a Nicotiana glauca-N. langsdorffii hybrid.
Cell division is one of the fundamental processes in all eukaryotes and plays a crucial role in growth and development of multicellular organisms. A 192-bp predicted DNA product was amplified from the genetic tumors of Nicotiana glauca-Nicotiana langsdorffii hybrid using degenerate primers specific for cyclin genes. A full-length cyclin cDNA clone was subsequently identified from the genetic tumors using a cDNA library screening strategy. In situ hybridization analysis showed that the GTcyc was detected in numerous, small spots in the whole range of genetic tumor.
Genetic simulated annealing algorithm coupled with niche is applied to search rock slope's critical slip surface in this study.Based on the slope structural plane simulation,the genetic simulated annealing algorithm coupled with niche can search the critical slip composed of the joints and rock-bridges.Compared with other intelligent algorithms,it has the merit of faster convergence rates and can find all extremes in a short time without strict requests for parameters.