A Practical Synthesis of The Antibiotic Toyocamycin
Abstract The nucleoside antibiotic toyocamycin was synthesized by condensation of the silylated 4-amino-6-bromo-5-cyanopyrrolo[2,3-d]pyrimidine with 1-O-acetyl-2,3,5-tri-O-benzoyl-D-ribofuranose, followed by debromination and deblocking.
Objective To study the best extracting technology of Compound Taizishen Granule prescription.Methods The total polysaccharides and total saponins were determined by UV spectrophotometer.With the content of total polysaccharides and total saponins as comprehensive indexes,orthogonal test was applied to investigation of the water addition,extraction time,and extraction times for optimizing the best extracting technology.Results The best extracting technology was using 10 times the amount of water,extracting 3 times and 1.5 h per time.Conclusion The extraction technology is safe and effective,which can be used as extraction for Compound Taizishen Granule prescription.