M-CSF, c-Fms, and Signaling in Osteoclasts and their Precursors
Prevention of conditions, such as osteoporosis, requires an understanding of the molecular mechanisms of bone resorption. The understanding that cells of the myeloid lineage are osteoclast precursors suggests that macrophage colony-stimulating factor (M-CSF) plays an important role in osteoclast biology. Signals generated by the binding of M-CSF to the cell-surface receptor c-Fms appear to trigger events leading to osteoclast differentiation. We have created a chimeric variant of the c-Fms receptor, which has allowed study of downstream events activated by M-CSF in a model more relevant to normal physiology than prior studies, which have relied on myeloid tissues. Our studies suggest novel regulatory signaling pathways initiated via the c-Fms receptor.
Minimum flowrate targeting methods for resource conservation networks (RCNs) have been developed over the last decades. The existing methodologies still have certain drawbacks. Their design insights could be deepened and some steps should be more convenient for the users. A targeting tool called the material surplus composite curve (MSCC), which is an improvement of the surplus diagram for water and hydrogen networks is introduced. The approach is illustrated on several cases selected from the literature. Using this technique, it is possible to determine rigorous flowrate targets for different variants of the RCN problem.