A simple procedure for the purification of yeast phenylalanine transfer RNA
Abstract For the past several years we have been attempting to study the secondary and tertiary structure of tRNAs + by means of chemical probes. In the course of this work it became necessary to obtain a highly purified species of tRNA. For two reasons we chose to purify yeast Phenylalanine tRNA. The nucleotide sequence of this molecule has been completely worked out ( RajBhandary and Chang [1968] ). Secondly, a simple and highly effective partial purification of this species has been obtained by Gillam et al. (1967) on benzoylated DEAE cellulose. In this communication we report the use of BD-cellulose to complete the purification of tRNA Phe .
Introduction A successful fi rst structure refi nement of lysozyme [1] already proved the value of standard XRPD laboratory instruments for protein structure research. In this contribution we will present new measurements and crystallographic analysis results of insulin and insulin derivatives performed on data from a PANalytical X’Pert PRO diffractometer used in different confi gurations. We additionally demonstrate that even fast measurements on a 96 well plate as used for polymorph screening purposes, result in high quality data, which is suitable for automatic crystallographic analysis like indexing [2] and LeBail [3] fi tting.