[Karyovolumetric studies on the pre-pubertal differentiation of the nucleus infundibularis in female rats].
Cell nuclear volumes of neurons located in the hypothalamic arcuate nucleus were measured in immature female rats autopsied at 21, 26, 32 or 36 days of age, or on the day of the first vaginal estrus. As opposed to limbic cell nuclei measured at the same ages in a former study (DOCKE aND SMOLLICH 1978), significant age-dependent changes of nuclear volumes could not be revealed with the exception of a continuous slight increase during the period of investigation. Neonatal ovariectomy whereas this treatment induced distinct activation of limbic neurons as recorded in a previous experiment (SMOLLICH et al. 1978). It is suggested that the puberty-controlling functions of the arcuate nucleus and of limbic structures depend on different mechanisms.
Background ::: A lack of consistent methods to evaluate Ki67 expression is problematic in terms of accurately predicting prognosis in breast cancer. Accordingly, this study aimed to identify the causes of discrepancies in Ki67 labeling index measurements by different observers under different conditions using breast cancer samples.