Development of Monoclonal Antibodies Reacting with Anisakis simplex Larvae
Anisakiasis is a common disease in Japan, since people eat raw fish as sushi and sashimi. Sushi is also becoming very popular in the United States and in some European countries with anisakiasis found in these places also, although the frequency is much lower when compared to Japan. The diagnosis of ansakiasis can be made from the history of eating raw fish, in addtion to physical findings, though such a final clinical diagnosis is not easy even with the development of gastrofiberscopical examination of patients.
Author contributions ::: ::: GH helped design the study, establish the animal model, collect and analyze samples, and write the manuscript. YY and DH helped establish the animal model, performed Western blotting, immunohistochemistry analyses. YY and DH also helped analyse data and revise the manuscript. QYW and JK contributed to study planning, analyse and interpret data, review and revise the manuscript. All authors have approved the final version of this article to be published and agreed to be accountable for all aspects of the work in ensuring the questions related to the accuracy or integrity of any part of the work. ::: ::: ::: ::: Disclosure ::: ::: The authors report no conflicts of interest in this work.