Synthesis of oligosaccharides and glycoconjugates
Synthesis and modification of carbohydrates using glycosidases and lipases.- Chemical transformations employing glycosyltransferases.- Enzymatic and chemical glycosylations of ergot alkaloids and biological aspects of new compounds.- Enzymatic synthesis of peptide conjugates - Tools for the study of biological signal transduction.- Tandem asymmetric C-C bond formations by enzyme catalysis.- The synthesis of novel sialic acids as biological probes.- Synthesis of oligosaccharides of bacterial origin containing heptoses, uronic acids and fructofuranoses as synthetic challenges.- Pyruvated saccharides - Novel strategies for oligosaccharide synthesis.
Objective To optimize the extraction technology of Yanqing Granule by orthogonal test.Methods Using the contents of chlorogenic acid and total extraction as indexes,choosing solvent volume,extracting duration and extracting times as influential factors,the optimal extraction condition was studied by L9(34) orthogonal test.Results The optimal extraction condition of Yanqing Granule was: 200 mL water for 1/3 amount of drugs,refluxing extraction for 3 times and 0.5 h each time.Conclusion The optimal extraction technology obtained in this study is simple and reproducible,and it has a higher application value in the production of Yanqing Granule.