A fast and simple method to determine the clonal relationship among human T-cell lymphocytes
Abstract For the analysis of mutation in human T-cell lymphocytes, it is crucial to determine the clonal relationship between isolated mutants, particularly if they harbour identical mutations. Here we report an efficient method to determine the clonal relationships between these cells. The method is based on the analysis of restriction fragment length polymorphisms of a polymerase chain reaction amplified, rearranged T-cell receptor γ-gene. As few as 600 cells are sufficient, regular agarose gels can be used for the separation of the restriction fragments, no radioactive label is required, and results can be obtained in 2 days.
Author contributions ::: ::: GH helped design the study, establish the animal model, collect and analyze samples, and write the manuscript. YY and DH helped establish the animal model, performed Western blotting, immunohistochemistry analyses. YY and DH also helped analyse data and revise the manuscript. QYW and JK contributed to study planning, analyse and interpret data, review and revise the manuscript. All authors have approved the final version of this article to be published and agreed to be accountable for all aspects of the work in ensuring the questions related to the accuracy or integrity of any part of the work. ::: ::: ::: ::: Disclosure ::: ::: The authors report no conflicts of interest in this work.