A theoretical approach to the prediction of the performance of batch operation of affinity cross-flow filtration (ACFF) for the separation of biomolecules is presented. The model applies general affinity theory to the ACFF system. Experiments were conducted using the affinity system of Cibacron Blue-agarose and human serum albumin (HSA). The predictions of the model agree well with the experimental results. The slow kinetics of release of the adsorbed protein during the washing stage can be utilized to minimize the loss of this protein.
Affinity ultrafiltration as a new filtration technology has both the advantages of affinity purification and membrane separation. In this study，the dextran T2000 (dextran with nominal molecular weight of 2 million) was employed，after epichlorohydrin activation and coupled with PAB（P-amino-benzamidine）as affinity ligand，we got affinity ultrafiltration carrier with the ligand concentration of 108.21µmol/g. The changes of infrared adsorption spectrum and ultraviolet adsorption spectrum in the processing of affinity carrier preparing were researched, and pilot study on trypsin purified by the affinity filtration was performed.
Berzelius failed to make use of Faraday's electrochemical laws in his laborious determination of equivalent weights.