what is the role of restriction enzymes in studying the human genome?
Restriction enzyme. A restriction enzyme or restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites.
EcoRI digestion produces sticky ends, whereas SmaI restriction enzyme cleavage produces blunt ends : Recognition sequences in DNA differ for each restriction enzyme, producing differences in the length, sequence and strand orientation (5' end or the 3' end) of a sticky-end overhang of an enzyme restriction. Different restriction enzymes that recognize the same sequence are known as neoschizomers.