On the Possible Direct Conversion of RNA to DNA
In the present study we have investigated the possibility that an RNA molecule can be directly converted in DNA by sequential action of ribonucleotide reductase- and thymidylate synthetase-like activities. The reasons for advancing such an unorthodox hypothesis arose from some considerations on the nuclear RNA (hnRNA) from eukaryotic cells.
Three molecular assays (FTD® Viral GE from Fast-track diagnostics, RIDA®GENE VSP1 from R-Biopharm, and Xpert Norovirus from Cepheid) were compared for virus detection in acute diarrhea samples. RIDA®GENE and FTD® Viral GE showed perfect/almost perfect agreement for Rotavirus, Sapovirus and Norovirus, substantial agreement for Adenovirus, and moderate agreement for Astrovirus.