Over-expression and Detection Analysis of Myostatin in Chicken Embryos Mediated by Retrovirus
Chick embryos have become a great experimental model system for developmental biology,immunology and cancer,etc.The chicken embryo and myostatin are used as model system and target gene respectively.The process of transferring gene into chicken embryo and detection are researched using retrovirus-mediated gene transfer and in situ hybridization.The experimental results show that target gene myostatin can be integrated into the embryonic genome,and has transcriptional activity;Whole-mount in situ hybridization can accurately reflect the virus integration sites and target gene expression levels.The study not only provides a base for further study myostatin functions in embryonic development,but also is a methodology attempt.
SUMMARY Ostrich production is highly management intensive. Losses to producers commonly arise from infertile eggs, poor egg handling, and incorrect storage and incubator settings (temperature, relative humidity, and air flow). Early chick mortality is also a significant factor influencing successful ostrich management. Microbial infection of ostrich eggs, caused by contaminated nests, inadequate egg cleaning, and poor incubator and hatcher sanitation, results in low hatchability. Adequate breeder nutrition is vital for ensuring fertility, increasing the number of eggs laid, and ensuring good survival rates of hatched chicks. The producer must work closely with veterinary extension officers, health laboratories, ostrich producer associations, researchers, and other farmers so that ostrich egg production is molded into a process of excellence.