Smoking cessation increases the resistance of low-density lipoprotein to oxidation
Abstract The effects of smoking cessation on the susceptibility to oxidation of low-density lipoprotein (LDL) was investigated in 14 men who quit smoking for 3 months. LDL was isolated and susceptibility of LDL to V-70 (4-methoxy-2,4-dimethylvalerinitrile)-mediated oxidation was assessed by measuring conjugated diene production at 234 nm, the lag phase being a measure of the resistance of LDL to oxidation. The mean duration of the lag phase became 1.9-fold longer after 3 months ( P
Abstract To identify changes in proteins accompanying transformation of normal lung epithelial cells to cancer cells, we performed a comparative proteomic study using two cell lines representing matching normal and cancer cells. Strikingly, a good number of detected actin cytoskeletal proteins were preferentially downregulated in cancer cells, while similar numbers of proteins in other organelles were up or downregulated. We also found that the formation of stress fibers and focal adhesions were substantially decreased in cancer cells. Protein network analysis showed that the altered proteins are highly connected. These results provide novel insights into the molecular mechanism promoting lung cancer progression.