Real-Time Quantitative PCR and Fast QPCR Have Similar Sensitivity and Accuracy with HIV cDNA Late Reverse Transcripts and 2-LTR Circles
Real-time fluorescent quantitative PCR (universal QPCR) methods are used routinely in both academic and clinical research to measure HIV cDNA. Fast QPCR allows for faster ramping times between cycles and smaller reaction volumes, but may lose sensitivity and accuracy. We demonstrate that primer sets for HIV late reverse transcripts and 2-LTR circles have similar sensitivity and accuracy with either universal or fast QPCR methods. However, both cost and time are reduced with fast QPCR.
With the development of internet and storage devices, online document servers abound with enormous quantities of documents, so that finding the right and useful information becomes a very difficult task. The end user, generally overloaded by information, can’t efficiently perceive such information. It became urgent to propose new information retrieval systems able to apprehend efficiently these enormous quantities of documents. In this paper we present PQIR an information retrieval model based on user profile. The originality of our approach is a choice of indexing terms depending on the user request but also on his profile. An empirical study confirms the relevance of our approach.