Donor tissue preparation for Descemet membrane endothelial keratoplasty.
We are pleased to note that Dr Zarei-Ghanavati and his team have replicated our technique (originally presented at the American Academy of Ophthalmology meeting in Atlanta 2008 and subsequently published)1 and agree that it is a viable, quicker and easier alternative to conventional donor preparation for Descemet membrane endothelial keratoplasty (DMEK) surgery.2 The process of pneumatic dissection described is essentially similar to ours; however, they have overlooked some key adaptations which further maximise the potential of this novel technique. ::: ::: We performed a superficial keratectomy using a 300 μm microkeratome head prior to performing the pneumatic dissection. In the …
Background ::: Spontaneously metastatic xenograft models of cancer are infrequent and the few that exist are resource intensive. In xenografts, caliper measurements can be used to determine primary tumor burden and response to therapy but in metastatic disease models determination of the presence of metastatic disease, metastatic burden, and response to therapy are difficult, often requiring serial necropsy. In this study we characterized the development of visceral metastases in a patient derived xenograft model (PDXM) using in vivo imaging.