Design of a unique PCR primer for detection of oral HPV infection.
Human papillomavirus (HPV) has been known as a pathogen of oral dysplasia. However, suitable PCR primers for the detection of oral HPV infection have not been reported. The aim of this study was to design unique consensus primers. The consensus primers were designed by homologues analyses between subtypes 2, 6, 11, 13, 16, 18, 30, 32 and 58, which frequently infect the oral membrane. PCR, with our designed primer, detected HPV DNA subtypes 2, 6, 11, 16, 18 and 58, and also showed PCR product from a clinical papilloma sample. These results indicate that our designed consensus primer can be used for the study of the relationships between oral disease and HPV infection.
This paper presents the low speed position estimation scheme for an IPM machine controlled by model predictive control with finite control set. The career signal injection is not viable as there is no PWM to superimpose it with PWM for this type of control. The pulse vector injection technique requires current derivative sensors which makes the overall scheme less attractive. This paper utilizes the inherent high frequency vector injection of the model predictive control to extract the position information. It is shown that the high frequency current response is amplitude modulated with respect to the position. A demodulation technique based on the reactive power estimation is proposed. The simulation results at various initial positions confirm the validity of the proposed position estimation scheme.