Analysis of phospholipids and fatty acids composition of rat hepatoma cells
Objective: To study the membrane phospholipid composition of rat hepatoma cells. Method: The mole fraction of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in total phospholipids of CBRH-7919 hepatoma cells was compared to that of normal liver cells by high performance thin layer chromatography (HPTLC) and phosphorus assay. The composition and mole fraction of fatty acids of PC in hepatoma cells were also compared to those of normal ones with gas chromatographic analysis (GC analysis). Results and Conclusion: The PC content of membrane phospholipids in CBRH-7919 tumor cells, particularly in long chain and unsaturated fatty acids was significantly lower than that of normal cells.
FIELD: chemistry. SUBSTANCE: invention concerns process of production of diisopropyl {[1-(hydroxymethyl)-cyclopropyl]oxy}methylphosphonate represented by the formula , which is the key intermediate compound in synthesis of antiviral nucleoside analogue. The invention also concerns new intermediate compounds of formulae and , and their production of compound (2) obtained under this invention, which is an antiviral nucleoside analogue (especially against hepatitis B virus) represented by the formula . EFFECT: high purity grade and high output. 4 ex