High Specificity Binding of Lectins to Carbohydrate-Functionalized Fiber Bragg Gratings: A New Model for Biosensing Applications
The functionalization of an etched fiber Bragg grating was realized using a carbohydrate-siloxane conjugate. No fluorescent probes were used. Concanavalin A bound with high specificity to the glucose biosensor, but not to the lactose functionalized fiber. Conversely, peanut agglutinin bound to the lactose sensor with high specificity over its glucose counterpart. Quasi-monolayer selective binding of the lectins to the fiber sensor was inferred based on a theoretical analysis of the observed changes in the refractive index. Our results open the way to the use of unlabeled carbohydrate-based sensors for the study of the human glycome.
Code generation with the Graham Glanville scheme can be improved, using backtracking parsers for attribute grammars. This eliminates semantic and syntactic blocking during code selection and guarantees the cheapest code possible, if desired. Multipass translation allows the use of nontrivial register allocation strategies. The code generator generator system BECC is a LALR(1) based backtracking parser generator for a derivate of S-attributed grammars which provides several interpreters that differ in their abilities and performance.