Specificity of Detection Is the Key Attribute of Selected Reaction Monitoring
Selected reaction monitoring uses tandem mass spectrometry to create instrumental conditions where only specific peptides can be detected. The two stages of the mass spectrometer are synchronized so that a signal is seen only when a molecular ion with a specific m/z is formed and that molecular ion fragments to a product ion with a specific m/z. The specificity of the analysis is increased by the unique amino acid sequence that defines a protein’s identity and the effects of that unique amino acid sequence on the other parts of the overall assay, including the protein digestion and liquid chromatography. The result is the ability to selectively measure, with confidence and accuracy, any protein in a complex mixture.
Abstract Wind is one of the fastest-growing renewable sources of electricity. As with most renewables, the economic viability relies on public subsidies. Even among renewables, the economic evaluation of wind projects is particularly challenging due to the unpredictability of the energy source, wind, and the high sensitivity of the project profitability to changes in a number of parameters. This makes the uncertainty analysis an important topic of research in the wind power sector. This study presents a method of combining two uncertainty analysis methods, sensitivity study and the Monte Carlo method, together with a technical and economic model of a wind farm, in an effort to improve the understanding of the practical effects of the uncertainties, and how they affect the financial risks of wind projects.