Determination of psoralens by gas chromatography
A gas chromatography capillary column determination of a mixture of psoralens is proposed. The validation of quantitative analysis is detailed and used for the evaluation of psoralens extraction from pharmaceuticals. Moreover stability in acidic or basic media is studied by LC/MS as well as the proposed gas chromatographic methods. A gas chromatography capillary column determination of a mixture of psoralens is proposed.
The study of rotifer phylogenies and the analysis of population-level processes historically have been disjunct. This is despite a growing recognition that there are many ways in which rotifer population biologists and ecologists might profit from the availability of a comprehensive phylogeny of the group. New molecular methods which can be applied to a wide range of genetic systems and systematic grades will shortly eliminate the methodological (and perhaps conceptual) distinction between these fields. Of particular importance is the development of the Polymerase Chain Reaction (PCR), a technique of synthetic DNA amplification which produces concentrated preparations of selected genes from complex mixtures of nuclear and mitochondrial genomes. Analysis of PCR products can provide hierarchal genetic comparisons from the level of local rotifer populations through broad evolutionary (at least molecular) phylogenies.