Un metodo di screening ELISA per la ricerca di 17α -etinilestradiolo in muscolo animale
The present paper describes an ELISA screening method for ethinylestradiol detection in animal muscle. The analyte is extracted from homogenized sample with organic solvent; the extract is defatted with petroleum ether, then purified on SPE C 18 column using an automatic system (ASPEC). Purified extract is used for ELISA test reading the optical density at λ=450 nm. Performance characteristics (specificity, CCβ and ruggedness) result satisfactory. Described procedure fit for purposes regarding the criteria defined by Decision 2002/657/EC for qualitative screening methods.
Author contributions ::: ::: GH helped design the study, establish the animal model, collect and analyze samples, and write the manuscript. YY and DH helped establish the animal model, performed Western blotting, immunohistochemistry analyses. YY and DH also helped analyse data and revise the manuscript. QYW and JK contributed to study planning, analyse and interpret data, review and revise the manuscript. All authors have approved the final version of this article to be published and agreed to be accountable for all aspects of the work in ensuring the questions related to the accuracy or integrity of any part of the work. ::: ::: ::: ::: Disclosure ::: ::: The authors report no conflicts of interest in this work.