Toll-Like Receptor 9 in Plasmacytoid Dendritic Cells Fails To Detect Parvoviruses
Toll-like receptor 9 (TLR9) recognizes genomes of double-stranded DNA (dsDNA) viruses in the endosome to stimulate plasmacytoid dendritic cells (pDCs). However, how and if viruses with single-stranded DNA (ssDNA) genomes are detected by pDCs remain unclear. Here we have shown that despite the ability of purified genomic DNA to stimulate TLR9 and despite the ability to enter TLR9 endosomes, ssDNA viruses of the Parvoviridae family failed to elicit an interferon (IFN) response in pDCs.
A healthy tissue tolerance study1,2 with Beagle dogs was carried out at the Finnish BNCT facility FiR 1 during February-March 1998. Three dose groups of dogs without boron carrier, each comprising five Beagles, and one dose group with BPA-F infusion were irradiated. Individual dose plans1 were done for each dog. The absorbed doses, which were actually delivered to healthy tissues, were monitored in vivo by placing the dosimeters on the shaved skin of the dogs or in the accessible cavities. In this study, absorbed gamma doses were monitored with thermoluminescent (TL) dosimeters, and neutron fluences were determined with activation foils. The obtained results were compared to the calculated values.