Midtrimester abortion with intramuscular injection of 15-methyl-prostaglandin E2
Abstract An intramuscular injection of 250 μ g of 15-methyl-PGF 2α was given to fifty patients every two hours until the fetus was expelled or for a maximum of twelve injections. Within 27 hours, 90% aborted with a mean injection-abortion time of 13.5 hours. All patients received prophylactic antiemetic and antidiarrheal medication. The side effects consisted of vomiting in 33 patients, diarrhea in 38 patients, flushing in 6 patients, and chills in 2 patients. Three patients had a blood loss ≥ 500 ml but did not require a blood transfusion. The intramuscular route offers the early midtrimester patient an effective and rapid means of abortion.
Chick embryos have become a great experimental model system for developmental biology,immunology and cancer,etc.The chicken embryo and myostatin are used as model system and target gene respectively.The process of transferring gene into chicken embryo and detection are researched using retrovirus-mediated gene transfer and in situ hybridization.The experimental results show that target gene myostatin can be integrated into the embryonic genome,and has transcriptional activity;Whole-mount in situ hybridization can accurately reflect the virus integration sites and target gene expression levels.The study not only provides a base for further study myostatin functions in embryonic development,but also is a methodology attempt.