Trisomy 12 correlates with elevated expression of p21ras in a human adenosquamous carcinoma of the lung
Abstract Reported herein is the first case in which a specific chromosomal abnormality, namely trisomy 12, was observed in primary cultures of a human adenosquamous carcinoma of the lung. Because of the c-K-ras had been shown to localize on chromosome 12, the relationship between trisomy and oncogene expression was examined in this tumor by concurrently assaying for the levels of ras oncogene protein product, p21, in both the tumor and adjacent normal tissues. An approximately tenfold increase in the level of p21ras was observed in the tumor, compared with the adjacent normal tissue. The present finding suggests a possible relationship between chromosomal trisomy and oncogene activation.
The long-term prognosis of Takayasu's disease depends on an elective surgical treatment which requires full and accurate assessment of the lesions. We present here a retrospective study of 50 patients explored before surgery by intra-arterial digital angiography. In 92 percent of the patients the thoraco-abdominal aorta and its branches were well documented on 2 orthogonal projections performed in one single session. The high-quality intra-arterial opacification made it possible to detect incipient parietal lesions and to evaluate the lesions of the small caliber branches and the downstream bed in case of occlusion. These advantages, and the absence of morbidity in our experience, make digital panarteriography a method of choice to evaluate Takayasu's disease.