1. A particle sorting system, comprising: laser light directed into a first laser interrogation region in first portion of a microchannel formed in a substrate; at least one particle manipulation stage formed in the substrate; and laser light directed into at least one additional laser interrogation region formed in a second portion of a microchannel formed in the substrate, wherein the first portion of the microchannel is disposed upstream of the particle manipulation stage, and the second portion of the microchannel is disposed downstream of the particle manipulation stage, and the particle manipulation stage comprises a movable structure microfabricated on the substrate which includes the microfluidic channels.