1. A single construct comprising: a first sequence comprising a first promoter and a sequence encoding an shRNA molecule, operably linked to the first promoter, wherein the shRNA molecule comprises a guide strand; and a second sequence comprising a second promoter and a target sensor, operably linked to the second promoter, the target sensor comprising: a sequence encoding a reporter and a target sequence that is co-transcribed with the sequence encoding the reporter, and the target sequence comprises from about 8 to about 29 contiguous nucleotides complementary to at least a portion of the guide strand of the shRNA molecule, such that the functional shRNA molecule is specific for the target sequence and is capable of suppressing reporter expression.