1. A method for processing a first digital image, representing a sample in a region, and which image has been acquired from the sample by means of a microscopic imaging system and is stored in a multi-resolution image data structure, comprising the steps of: retrieving a sub-region of the first digital image at a first resolution, executing a transform function on the retrieved sub-region, the transform function modifying a content of the sub-region according to at least one metric derived from a second resolution representation of the first digital image.