1. A method for determining a measure of the concentration of analyte molecules or particles in a fluid sample, comprising: providing a plurality of a first type of capture objects that each are associated with either at least one analyte molecule or particle or are free of any analyte molecules or particles; providing a plurality of a second type of capture objects that are free of any analyte molecules or particles, wherein the second type of capture objects are distinguishable from the first type of capture objects; addressing at least a portion of the first type of capture objects and determining the percentage associated with at least one analyte molecule or particle; addressing at least a portion of the second type of capture objects and determining at least one signal; and based upon the percentage, either determining a measure of the concentration of analyte molecules or particles in the fluid sample based at least in part on the percentage or particle or determining a measure of the concentration of analyte molecules or particles in the fluid sample based at least in part on the at least one signal and a measured intensity of a signal that is indicative of the presence of a plurality of analyte molecules or particles.