1. A calibration method of a stereo camera, wherein a calibration system applied to the calibration method comprises a host and a display, and the stereo camera comprises a left eye image capture unit and a right eye pattern capture unit, the calibration method comprising: the host optionally setting a plurality of camera calibration parameters and a plurality of image rectification parameters of the stereo camera; the left eye image capture unit and the right eye pattern capture unit executing an image capture step on at least one left eye pattern and at least one right eye pattern generated by the host corresponding to each two-dimensional pattern of at least one two-dimensional pattern, respectively, wherein the image capture step comprises: the display displaying the at least one left eye pattern, wherein each left eye pattern of the at least one left eye pattern corresponds to a predetermined angle and a predetermined distance; the left eye image capture unit capturing a first image corresponding to the each left eye pattern; the display displaying the at least one right eye pattern, wherein each right eye pattern of the at least one right eye pattern corresponds to the predetermined angle and the predetermined distance; and the right eye pattern capture unit capturing a second image corresponding to the each right eye pattern; the host generating a plurality of new camera calibration parameters according to a plurality of first images corresponding to all left eye patterns of the at least one two-dimensional pattern and a plurality of second images corresponding to all right eye patterns of the at least one two-dimensional pattern after the image capture step is executed on the at least one left eye pattern and the at least one right eye pattern corresponding to the each two-dimensional pattern; and the host generating a plurality of new image rectification parameters according to the plurality of new camera calibration parameters.