1. A cartridge comprising: a bottom layer, wherein, at least a portion of the bottom layer is non-porous; a sample distribution layer; and at least two test sites having at least two test site read zones; wherein the test sites are adjacent to or embedded within the sample distribution layer and configured to detect at least two analytes using two different techniques, and wherein the locations of at least one of the at least two test zones are determined by performing the detection or quantification at the at least one of the at least two test read zones.