1. A method of biometric acquisition, comprising the steps of: projecting light, by an illuminator, of a predetermined wavelength onto an iris and a non-iris region; measuring a reflectance of the iris and a reflectance of the non-iris region based on light reflected off the iris and the non-iris region; comparing a ratio of the reflectance of the iris to the reflectance of the non-iris region, with a reflectance ratio pre-determined from a live eye using light of the same wavelength; and determining that the iris is from a live eye if the ratio of the measured reflectances is consistent with the pre-determined reflectance ratio.