1. A method for determining a relative concentration of a specific fluorophore associated with an object, comprising the steps of: (a) focusing light from an object along a collection path, the object comprising at least two different fluorophores; (b) dispersing the light that is traveling along the collection path into a plurality of light beams, such that each light beam corresponds to a different wavelength; (c) focusing each of the light beams to produce respective images for the light beams; (d) providing a detector disposed to receive the respective images and in response, generating an electrical signal; (e) processing the electrical signal to separate the electrical signal into portions corresponding to each one of the at least two different fluorophores; and (f) deriving an amplitude for each portion of the electrical signal that corresponds to one of the at least two different fluorophores, the amplitude for each fluorophore corresponding to a relative concentration for that fluorophore.