1. A transformant of a fungus belonging to Aspergillus , comprising, a first transformation marker gene fragment with a 3′ terminal deficiency integrated in a reverse direction into the 5′ end of a target region capable of duplicated translocation wherein the target region is located in a long arm of a first chromosome, or said marker gene fragment is integrated in a forward direction into the 3′ end of a target region capable of duplicated translocation wherein the target region is located in a short arm of the first chromosome; and a second transformation marker gene fragment, which is the same as the first transformation marker gene fragment, except that it has a 5′ terminal deficiency, integrated in a reverse direction into the 5′ end of a region to be replaced with one of said target regions wherein the region to be replaced is located in a long arm of a second chromosome, or said marker gene fragment is integrated in a forward direction into the 3′ end of a region to be replaced with one of said target regions wherein the region to be replaced is located in a short arm of the second chromosome; wherein a homologous sequence region is present in common in a middle portion of a coding region of both of the transformation marker gene fragments.