1. A system for three dimensional imaging of motile objects contained within a sample comprising: an image sensor; a sample holder configured to hold the sample, the sample holder disposed adjacent to the image sensor; a first illumination source having a first wavelength and positioned relative to the sample holder at a first location to illuminate the sample; a second illumination source having a second wavelength, different from the first wavelength, and positioned relative to the sample holder at a second location, different from the first location, to illuminate the sample; and wherein the first illumination source and the second illumination source are configured to simultaneously illuminate the sample contained within the sample holder.