1. A navigation system, comprising: a patient holding device configured to hold a patient fixed in a substantially single holding position relative to an imaging device during acquisition of image data of the patient; a navigation reference frame removably fixed to the patient holding device to remain in a substantially single reference frame position relative to the patient during the image data acquisition and a procedure on the patient; a plurality of navigation reference frame imageable portions included with the navigation reference frame and viewable in acquired image data of the patient when the plurality of navigation reference frame imageable portions are in a field of view of the imaging device; a tracking device included with the navigation reference frame operable to be tracked and at a known location relative to the plurality of navigation reference frame imageable portions; and a navigation processor system configurable to execute instructions to determine a location of the plurality of navigation reference frame imageable portions in the acquired image data, determine a subject space location of the tracking device, and register the acquired image data to the subject space based at least on the acquired image data of the plurality of navigation reference frame imageable portions.