1. A method for analyzing a digital pathology image, the method comprising: selecting at least one analysis region in the digital pathology image; separating the at least one analysis region into a tissue region and a background region using a predetermined separation threshold; selecting nuclei within the tissue region; determining an estimated membrane perimeter; designating a membrane region according to the tissue region and the selected nuclei; calculating an actual membrane perimeter of the membrane region; and calculating a staining extent by comparing the estimated membrane perimeter and the actual membrane perimeter.