1. A system for imaging a biological sample comprising: a light source that emits excitation light that is optically coupled onto a region of interest of the biological sample; a scanning device optically coupled to the light source such that light is scanned onto the region of interest, the scanning device being operated in response to light scan parameters; a controlled stage to support the biological sample to be sectioned into a plurality of layers from the biological sample after imaging of each layer in sequence, the controlled stage being operated in response to selected stage scan parameters; a sectioning device such that relative movement of the controlled stage and the section device in response to control signals is operative to section the biological sample into the plurality of layers; an image sensor that receives light from the biological sample to detect an image of the region of interest within each of the plurality of layers to generate a plurality of images; and a data processor that processes the plurality of images to produce stored electronic image data of the region of interest.