1. A method for mapping anatomical structures and marking them on a substrate for a surgical procedure, comprising: inserting an imaging device into a surgical site; obtaining an image of a defect located in the surgical site from the imaging device; identifying at least one anatomical feature of the surgical site and marking the at least one anatomical feature on the image; setting a minimum margin to be maintained between an edge of the defect and an edge of the substrate; measuring the defect; transmitting the image to a printer; printing the image on the substrate; inserting the substrate into the surgical site, wherein the substrate includes the image and the at least one anatomical feature is printed on the substrate as a corresponding landmark; and positioning the substrate over the defect such that the corresponding landmark aligns with the at least one anatomical feature.