1. A method for the analysis of an image of a biological sample comprising one or more candidate objects of interest), comprising the steps of: obtaining an image of the biological sample; separating the image of the biological sample into a First Image and a Second Image; assimilating the morphological features and/or morphological pattern of the one or more candidate objects of interest in the First Image; applying to the Second Image the morphological features and/or morphological pattern assimilated from the First Image; eliminating from the Second Image objects with non-matching morphological features and/or non-matching morphological patterns with the one or more candidate objects of interest in the First Image; and quantitating the level of expression of the one or more candidate objects of interest in the First Image using the matching morphological features and/or morphological patterns of the one or more candidate objects in the First Image and the Second Image.