1. A system, comprising: an imaging subsystem configured to image, at different wavelength bands, particles disposed within the imaging subsystem, wherein the imaging comprises: selecting a first set of one or more optical filters corresponding to a first wavelength band; illuminating the particles through the first set of optical filters; selecting a second set of one or more optical filters corresponding to a second wavelength band; and illuminating the particles through the second set of optical filters; and a data processing subsystem configured to: store data acquired for multiple images of the particles, and wherein particular images of the multiple images include spots corresponding to the particles, wherein a first image of the particular images corresponds to the first set of optical filters, wherein a second image of the particular images corresponds to the second set of optical filters; create a first composite image of the multiple images, wherein the first composite image includes first composite spots corresponding to the particles, the first composite spots having a first amount of misalignment from the spots in the particular images; and modify coordinates of at least one of the multiple images such that a second composite image based on the modified coordinates includes second composite spots having a second, smaller amount of misalignment from the spots in the multiple images.