1. A method for compensating an image for waveguiding effects, the method comprising: (a) detecting excitation light emanating from at least part of a heterogeneous diffuse object at one or more wavelengths, thereby acquiring an intrinsic image; (b) detecting emission light emanating from at least part of said heterogeneous diffuse object at one or more wavelengths, thereby acquiring a detected emission image, said emission light emitted by an imaging probe located within or on said heterogeneous diffuse object as a result of excitation by said excitation light; (c) creating an estimated emission image using at least said intrinsic image; and (d) altering said detected emission image using at least said estimated emission image, thereby compensating said detected emission image for waveguiding effects.