1. A plurality of microdroplets, each comprising a single nucleic acid template and a plurality of species of primer pairs, each species of primer pairs being specific for a different target site, wherein each of the single nucleic acid templates in the microdroplets comprise one of the different target sites that is recognized by only a single species of primer pairs, wherein the single species of the primer pairs binds to the target site of the single nucleic acid template in each microdroplet.