1. A method of processing data associated with fluorescent emissions from a microfluidic device, the method comprising: performing an auto-focus process associated with a first image of the microfluidic device; performing an auto-exposure process associated with the first image of the microfluidic device; capturing a plurality of images of the microfluidic device, wherein the plurality of images are associated with a plurality of thermal cycles; performing image analysis of the plurality of captured images to determine a series of optical intensities; and performing data analysis of the series of optical intensities to provide a series of changes in threshold values.