1. A method for determining profile data for an edge feature in a machine vision inspection system, the machine vision inspection system comprising: an edge feature video tool comprising a region of interest defining portion, the edge feature video tool configured to determine profile data for an edge feature in the region of interest based on a plurality of differently focused images of the region of interest; the method comprising: placing a workpiece in a field of view of the machine vision inspection system; operating the edge feature video tool to define a video tool region of interest including an edge feature in an acquired image of the workpiece; and operating the edge feature video tool to analyze image data of at least one workpiece image in the region of interest in order to provide an edge-referenced alignment compensation related to substantially minimizing a respective offset amount of the edge feature at respective locations along a directional filtering direction used for directionally filtering the plurality of differently focused images prior to determining the profile data for the edge feature.