1. A method executable by an automated microscopy platform for measuring cell activity represented in an image of cells treated with an agent, the image obtained with the automated microscopy platform, comprising: segmenting cellular components in the image; separating segmented components of overlapping cells in the image; and determining, in the segmented, separated image, translocation of cellular material between first cellular compartments and second cellular compartments caused by the agent, by: measuring a first intensity of the cellular material in a first cellular compartment a of a cell; measuring a second intensity of the cellular material in a second cellular compartment b of the cell; and, determining fractional localized intensity F a of the cellular material in the first cellular compartment according to a ratio of the first intensity to a sum of at least the first and second intensities, according to: F a = ∑ ( x , y ) ∈ a ⁢ I ⁡ ( x , y ) ∑ ( x , y ) ∈ a , b ⁢ I ⁡ ( x , y ) where I(x, y), the emission intensity at a pixel location (x, y) in a cellular compartment, is determined according to: I ⁡ ( x , y ) = ∫ 0 z ⁢ I 0 ⁢ Q ⁢ ⁢ ɛ ⁢ ⁢ u ⁢ ⁢ ⅆ z in which I 0 is an incident excitation intensity, Q is a quantum yield, ε is an extinction coefficient, u is a local fluorophore concentration at pixel location (x, y), and z is the thickness of a column of cellular material at pixel location (x, y).