1. A computer-implemented method of creating an image of a specimen, the method comprising: receiving, by a processor, a first image of a first section of a specimen, the first image created using a first wavelength of invisible light; receiving, by the processor, a second image of a second section of the specimen, the second section being adjacent to the first section and the second image created using the first wavelength of invisible light; co-registering, by the processor, the first image and the second image; creating, by the processor, a single-plane image of the first section using a next-image process; de-blurring, by the processor, the first image; and determining at least one tissue type from a visible light image and wherein the de-blurring is performed based on the at least one tissue type, wherein the specimen is contacted with at least one first fluorophore having an emission spectrum in the range of approximately 200 nm to 1000 nm.