1. An image acquisition device comprising: optics configured to magnify a specimen on a microscope slide to a diagnostic resolution; a stage configured to support the microscope slide; at least one processor communicatively coupled with at least one computer readable storage medium, wherein the processor is programmed to create three dimensional digital slides by: receiving a set of three dimensional scan parameters comprising a length, width and depth; scanning a three dimensional Z-stack in accordance with said length, width and depth, the Z-stack comprising a plurality of Z-plane images; and storing the three dimensional Z-stack as at least a portion of a three dimensional digital slide image.