1. A non-transitory computer-readable storage medium storing one or more computer programs adapted to cause a processor based system to execute steps comprising: converting at least a part of image information of a tissue sample to an optical domain image by separating the at least a part of image information into positive stain image information and negative stain image information; identifying a number of positive stained nuclei based on the positive stain image information and a number of negative stained nuclei based on the negative stain image information; calculating a positive stained nuclei percentage based on a ratio of positive stained nuclei identified based on the positive stain image information to total nuclei included in the at least a part of image information, wherein a percentage score is calculated based on the positive stained nuclei percentage; and displaying an indication of a cancer diagnosis score derived at least in part from the percentage score.