1. A calibration assembly for use with an endoscopic imaging system having a field of view, the assembly comprising: an interface configured for constraining engagement with the endoscopic imaging system; a target coupled with the interface so as to be within the field of view and reconfigurable from a first relative spatial arrangement between the imaging system and the target to a second relative spatial arrangement between the imaging system and the target, wherein the target includes a plurality of markers having calibration features that include identification features; and a processor coupled with the imaging system and configured to identify, from first and second images obtained at the first and second relative spatial arrangements, respectively, at least some of the markers from the identification features, the processor generating calibration data by using the identified markers and calibration feature positions within the images.