1. A transformant of a fungus belonging to Aspergillus which is characterized by that a transformation marker gene with deficiency of a terminal part at the 5′ end of its coding region is integrated into an outside of the centromere side of a target region in a chromosome of the fungus subject to duplicated translocation wherein a 5′ terminal of the marker gene is located in the centromere side, and a transformation marker gene with deficiency of a terminal part at the 3′ end of its coding region is integrated into an outside of the centromere side of a region in another chromosome of the fungus to be replaced with the target region wherein a 5′ terminal of the marker gene is located in the centromere side.