1. A method for three dimensional reconstruction of objects of interest comprising the steps of: (a) injecting objects of interest into a laminar flow stream such that objects are centered in the laminar flow stream and moving at a constant velocity; (b) sampling the objects of interest with at least one optical point source located around the laminar flow stream, in cooperation with at least one opposing time delay and integration (TDI) image sensor located opposite the at least one optical point source at a distance from the laminar flow stream such that there is no focal plane within the objects of interest during sampling, and where multiple projection angles through objects of interest are sampled as they flow between the at least one optical point source and at least one opposing optical sensor; and (c) generating at least one projection image with the at least one TDI image sensor, the line transfer rate of which is synchronized to the flow rate of the objects of interest.