1. A method for processing an image of a microfluidic device, the method comprising: receiving a first image of a microfluidic device, the first image corresponding to a first state; receiving a second image of the microfluidic device, the second image corresponding to a second state; transforming the first image into a third coordinate space, using a processor, the transforming using at least a first fiducial on the first image; transforming the second image into the third coordinate space, using the processor, the transforming using at least a second fiducial on the second image; obtaining a third image based on at least information associated with the transformed first image and the transformed second image; processing the third image to obtain information associated with the first state and the second state using the processor.