1. A non-transitory computer-readable storage medium storing one or more computer programs adapted to cause a processor based system to execute steps comprising: converting at least a part of image information of a tissue sample to an optical domain image; separating the at least a part of image information into positive stain image information and negative stain image information; identifying a number of positive stained nuclei based on the positive stain image information and a number of negative stained nuclei based on the negative stain image information; calculating a number of total nuclei by summing the number of positive stained nuclei and the number of negative stained nuclei, wherein a percentage score is calculated based on the number of positive stained nuclei and the number of total nuclei; and displaying an indication of a cancer diagnosis score derived at least in part from the percentage score.