1. A method of enhancing 3D image information density, comprising: providing a fluorescent microscope and a rotational stage; collecting 3D image samples at different angles; performing a deconvolution process for said 3D image samples by a processing unit, wherein said performing a deconvolution process comprises performing a blind deconvolution to estimate a point spread function; performing a registration process of said deconvoluted 3D image samples by said processing unit, wherein said performing a registration process comprises generating SIFT (Scale Invariant Feature Transform) matched features of every image of said 3D image samples; and performing an interpolation process of said registered 3D image samples by said processing unit to output a 3D image in high resolution.