1. A method for generating a graphical user interface on a display of a computer system for performing a profile analysis, comprising: displaying an electronic microscope image in the graphical user interface on the display; providing controls in the graphical user interface on the display for adjusting the electronic microscope image; providing a graphical measurement control in the graphical user interface on the display; and providing controls in the graphical user interface on the display for calibrating the graphical measurement control, wherein the controls for calibrating the graphical measurement control include a first calibration slider and a second calibration slider, and a calibration distribution select field to enable user assignment of a distance value to a displayed distance between the first and second calibration sliders, wherein the first and second calibration sliders are displayed in the graphical user interface along an edge of the displayed electronic microscope image, and wherein the first and second calibration sliders are independently movable in a linearly constrained manner along the edge of the displayed electronic microscope image.