1. Data generation method for disease determination for generating data for disease determination using a biological sample obtained from the subject, comprising; pretreatment process to add an acidic or alkaline solution to the sample and to heat the sample, excitation light irradiation process to irradiate the sample with the excitation light and to continuously or intermittently change the wavelength of the excitation light, emission light measurement process to measure the wavelength and intensity of the emission light emitted from the sample in response to the excitation light, and analyzing and sorting process to detect a specific point in the three-dimensional optical spectrum composed of the excitation light wavelength, emission light wavelength and emission light intensity and to sort the sample based on an attribute of the specific point, wherein the specific point is a relative maximum peak or a relative minimum peak of a fluorescence intensity and is utilized for disease determination.