1. A method of generating a fly-through review for a digitally imaged tissue sample, said method comprising: determining a fly-through path based on a user-selected fly-through starting point and a computed segmentation of a tissue boundary in said sample image to generate one or more fly-through curves; applying, using a processor, two-dimensional image manipulations to said sample image in accordance with said one or more fly-through curves to generate a sequence of frame images to be displayed in an automatic fly-through review; and displaying the automatic fly-through review to the user.