1. A method, comprising: introducing an assay into a static imaging optical analysis system, wherein the assay comprises (i) one or more analytes of interest, and (ii) at least two discrete particle sets distinguishable at least by their distinct concentrations of particles within the assay and the discrete ranges of magnetic material coupled to the particles of the respective particle sets; routing the assay in proximity to an, imaging plane within the static imaging optical analysis system; generating a magnetic field in proximity to the imaging plane that is sufficient to attract and immobilize a ratio of particles from the at least two discrete particle sets that is different from the ratio of particles comprising the at least two discrete particle sets in the assay as the assay is introduced into the static imaging optical analysis system; imaging the particles immobilized on the imaging plane; and analyzing one or more images produced from the step of imaging to determine the presence, absence and/or concentrations of the one or more analytes of interest within the assay.