1. A computer-implemented method for quantifying an object in a larger structure using a reconstructed image obtained from an imaging apparatus, said method comprising: identifying an object boundary in a region of interest of the image and a region of exclusion; determining a mean value of intensity in a neighborhood of the object to estimate a background value, excluding the region of exclusion; determining an interior region, a transition region, and a background region of the object using the object boundary and a transfer function of the imaging apparatus; refining the object boundary using a system resolution of the imaging apparatus, the system resolution having a predefined number of voxels; determining a measure of object quantification utilizing intensities of at least one of the determined interior region or the transition region, wherein said determining a measure of object quantification further comprises determining a volume of interest as a connected component list (CC) plus a number of surrounding voxels that are a multiple of the predefined number of voxels of the system resolution; and displaying the measure of object quantification to a user.