1. A method of obtaining three dimensional (3D) optical polarization tractography (OPT) images of 3D fiber orientation inside a target tissue, the method comprising: Making an initial OPT image of fiber orientation of a target tissue sample using an incident light source thereby producing a first image volume; Making at least one additional OPT image of the fiber orientation of the target tissue using one or more projection planes different from the initial fiber orientation thereby providing dual-angle imaging data and producing at least a second image volume; Determining an intersection line of the at least two different projection planes for points inside the sample, whereby the 3D image properties of the target tissue become known; and Transmitting the 3D image properties of the target tissue to an OPT processor to produce a 3D image of the target tissue.