1. A substrate inspection device, comprising: a device main body, on a surface of which is movably disposed with a shifting unit and above the surface of which is suspended a lifting unit, wherein a light emission element is disposed on a surface of the shifting unit; a bearing module, which is suspended above the surface of the shifting unit and has an opening, wherein a plurality of clamping units are movably disposed on a surface on an opposite side from a surface opposing to the shifting unit and can movably clamp a substrate; an illuminating and image capturing module, which is disposed on the lifting unit; a control module, which is electrically connected to the shifting unit, the lifting unit, and the illuminating and image capturing module, wherein the control module receives a first inspection signal and accordingly drives the lifting unit to move in a first direction, such that the illuminating and image capturing module moves closer to the substrate, and the control module controls the shifting unit to drive the light emission element to project a first spot-light through the opening on the substrate and controls the shifting unit to move by a step manner in a second direction or in a third direction so as to carry the bearing module to move, and the control module controls the illuminating and image capturing module to capture images of a plurality of first regions of the substrate and to generate a plurality of first images; and an illuminating module, which is movably disposed on the device main body and is electrically connected to the control module, and a condenser unit is disposed on the illuminating module, wherein the control module receives a third inspection signal and accordingly controls the illuminating module to move towards the bearing module, such that the condenser unit moves to a position between the shifting unit and the bearing module and corresponding to the illuminating and image capturing module, and the control module controls the shifting unit to move by the step manner in the second direction or the third direction so as to carry the bearing module to sequentially move to a plurality of preset locations and controls the condenser unit to sequentially project focused light through the opening on one of a plurality of apertures of the substrate, and the control module controls the illuminating and image capturing module to sequentially capture images of the plurality of apertures and to generate a plurality of aperture images.