1. A method for processing a first digital image, representing a sample in a region, which first digital image has been acquired from the sample by a microscopic imaging system and stored in a multi-resolution image data structure, the method comprising: computing a transform function according to at least one metric derived from a second digital image and a corresponding metric derived from the first image, the second digital image having a sub-region with a contour; retrieving a sub-region of the first digital image at a first resolution, the sub-region of the first digital image having a contour defined by the transformed contour of the sub-region of the second digital image; and executing the transform function on the retrieved sub-region of the first digital image, the transform function modifying a content of the selected sub-region of the first digital image according to at the least one metric derived from a second resolution representation of the first digital image.