1. A method of obtaining at least one obscured or partially obscured population analysis from at least a portion of a sample having at least a first cell population including at least one population subset of interest and a second cell population, comprising: electronically sensing and counting a first population including at least the first cell population and subsets thereof to form a first count; shifting the cell population subset of interest out of said first cell population and at least partially into a second cell population by binding microspheres having a reactant bonded thereto specific to said cell population subset of interest to said cell population subset; electronically sensing and counting the remaining first population including at least the first cell population without the shifted subset and with remaining subsets thereof to form a second count; and comparing said first and second counts to obtain the percentage contribution of the cell population subset of interest.