1. A method of imaging a tissue sample comprising: illuminating a first region of tissue with light; detecting light from the tissue in response to the illuminating light to form an image of the first region of tissue; sectioning a portion of the imaged tissue to expose a second region of tissue; illuminating the second region of tissue; detecting light from the second region of tissue to form an image of the second region of tissue; sectioning a portion of the second region of tissue; processing the first section of tissue and the second region of tissue; imaging the processed first section of tissue and the processed second section of tissue to form processed tissue images; and analyzing the processed tissue images in combination with the images of the first region of tissue and the second region of tissue.