1. A method of quantitative analysis of cell nuclei, comprising: capturing an image of cell nuclei; segmenting the image to identify individual nuclei; fitting three intensity peaks of the pixels of the image of individual nuclei to the histogram of number of counts against image intensity, the three intensity peaks being low, middle and high intensity peaks and being represented by fitting parameters; determining characteristic data, wherein some of the characteristic data is determined from the fitting parameters'; and separating the image of the individual nuclei by intensity of pixels to create a first image of a first object made up of low intensity pixels representing the area of dark chromatin and a second image of a second object of higher intensity pixels representing the area of light chromatin.