1. A three-dimensional video content generation method, for generating a three-dimensional video signal, comprising: (A) receiving a two-dimensional image and generating a region of interest distribution map from the 2D image; (B) executing a color feature capture process, for forming a plural of color feature regions; (C) executing an image segmentation process basing on the texture feature of the plural of color feature regions, for forming an image region distribution map; (D) executing a depth map generation process, for generating a depth map basing on the region of interest distribution map and the image region distribution map; (E) executing a three-dimensional image generation process, for forming the three-dimensional image basing on the image region distribution map and the depth map; and (F) chaining a plurality of the three-dimensional images to form the three-dimensional video basing on a frame rate.