1. A method of determining a sub-pixel point position of a marker point in an image, the method executed by an imaging system including a scanner, a computer with an electronic processing unit, and a memory module storing a marker point sub-pixel localization module executable by the electronic processing unit, the method comprising: obtaining, at the computer, an image based on image data generated by the scanner, the image including a marker point; deriving, with the electronic processing unit, a background corrected marker point profile; deriving, with the electronic processing unit, a background and baseline corrected marker point profile; defining, with the electronic processing unit, a sub-pixel position of the marker point as a center of a peak of the background corrected marker point profile.