1. A method for imaging tissue, comprising the steps of: mounting the tissue on a computer controlled stage of a microscope; determining volumetric imaging parameters; directing at least two photons onto a region of interest; scanning the region of interest across a portion of the tissue; imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest; sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volume of the tissue in the region of interest; detecting an image of the tissue due to said excitation light; and processing three-dimensional data that is imaged to create a three-dimensional image of the region of interest.