1. A method for inspecting a material applied in at least one predefined pattern on part of a substrate to determine an extent to which the material overlays regions of interest on the substrate, the method comprising: providing pre-application image data representative of the regions of interest and positions of the regions of interest on the substrate before application of the material; acquiring a post-application image of the part of the substrate after application of the material; aligning substantially the post-application image with the pre-application image data to provide an aligned post-application set of characteristics; processing the aligned post-application set of characteristics to generate a post-application material image representative of the material applied in the at least one predefined pattern on the part of the substrate; segregating the regions of interest of the post-application material image using the pre-application image data to provide on-region information representative of a position of the material on the regions of interest; and analyzing the on-region information to determine the extent to which the material overlays each of the regions of interest.