1. A method of generating an in-focus image of a measurement location of a sample holder in a microscopy imaging system, the method comprising: (a) positioning a plurality of imaging devices at a first distance from the sample holder, wherein an imaging device of the plurality of imaging devices corresponds to a respective measurement location; (b) acquiring a first set of images at respective measurement locations using the plurality of imaging devices; (c) developing a set of candidate output images associated with the plurality of imaging devices in accordance with the first set of images; (d) moving the plurality of imaging devices by a predetermined distance relative to the sample holder; (e) acquiring a second set of images at the respective measurement locations using the plurality of imaging devices; (f) updating at least a portion of the respective ones of the set of candidate output images with at least a portion of the respective second set of images in accordance with a selection criterion; and (g) transmitting the set of updated candidate output images, wherein the steps of moving the plurality of imaging devices, acquiring the second set of images and updating respective ones of the set of candidate output images are undertaken sequentially a plurality of times before the set of updated candidate output images is transmitted.