The serum samples were harvested on post burn day ( PBD ) 3 , 7 , 14 for determination of the levels of gastrin ( GAS ) by radioimmunoassay , motilin ( MTL ) by enzyme-linked immunosorbent assay , diamine oxidase ( DAO ) by enzyme spectrophotometry , and endotoxin ( ET ) by kinetic turbidimetric assay with TAL .
