To ( 1 ) evaluate the use of adenosine triphosphate ( ATP ) - driven bioluminescence for quantification of total plaque bacteria in orthodontic patients , ( 2 ) compare plaque bacteria amounts at the bracket-tooth interface with use of elastomeric-ligated and self-ligating brackets after 1 year of orthodontic treatment , and ( 3 ) analyze formation of white spot lesions by photographic evaluation and laser-light fluorescence ( DIAGNOdent ) .
