Anti-AMA1 immunoglobulin G ( IgG ) were detected by enzyme-linked immunosorbent assay ( ELISA ) , and the immune sera of volunteers that received 20 microg or 80 microg of AMA1-C1 / Alhydrogel + CPG 7909 had up to 14 fold significant increases in anti-AMA1 antibody concentration compared to 80 microg of AMA1-C1 / Alhydrogel alone .
