Fifty-nine healthy adolescents , 12-14 years old , undergoing orthodontic treatment with fixed appliances and harbouring high levels of MS in saliva and preferably also in interdental plaque , were randomly distributed into four groups , using dentifrices with : 1 ) zinc lactate ( n = 16 ) , 2 ) amine fluoride-stannous fluoride ( n = 13 ) , 3 ) triclosan ( n = 15 ) , and 4 ) no antimicrobial agent ( control ; n = 15 ) .
